Rhonda Ann Patterson

Transcription

1 Rhonda Ann Patterson 08/2014 Curriculum Vitae Address Biology Department 1161 Beaver Dam Church Road Ogden College of Science & Engineering Brownsville, KY Western Kentucky University Nashville Road, Suite B Bowling Green, KY (phone) rhonda.patterson@wku.edu Born Carthage, Missouri Education B.S. Ag. (1987) in Animal Science from University of Missouri-Columbia; Columbia, MO Ph.D. (1998) in Immunology from University of Southern Mississippi; Hattiesburg, MS Fellowships Patricia Roberts Harris Fellowship Honors Who s Who Among America s Teachers (2004 and 2005) Professional Society Activities International Association of Aquatic Animal Medicine Past-President ( )- Responsible for serving on the Nominations and Awards committee (this year and the next two) as well as one final year on the Executive Board. Responsible for filling in for the President if for any reason he is not able to fulfill his duties. President ( ) - Responsible for overseeing all of the activities of the organization. Run all Executive Board meetings and the annual business meeting. Write all official correspondence for the organization requiring presidential seal. President-Elect ( ) - Responsible for planning scientific program and coordinating with host institution for 36 th Annual Conference of the International Association for Aquatic Animal Medicine, May, 2005, Seward, Alaska. Newsletter Editor and Executive Board member - starting fall, 2000.

2 Chair - Pre-Conference all day workshop on "Immunology for the Aquatic Animal Clinician" at the 34th Annual Conference of the International Association for Aquatic Animal Medicine, May, 2003, Waikoloa, Hawaii. Associate Editor - Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, September 2000, New Orleans, LA. Chairperson ( ) - Conservation and Governmental Affairs Committee of the International Association of Aquatic Animal Medicine. Co-chair - Pre-Conference Marine Mammal Immunology Workshop for Clinicians at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Member present Society for Marine Mammalogy (Member ) International Society of Developmental and Comparative Immunology (Member ) American Association for Women in Community Colleges (Member ) Association of College & University Biology Educators (Member 2007-present) National Association of Biology Teachers (Member 2007-present) The National Association for Developmental Education (Member 2007) National Science Teachers Association (Member present) Society Memberships Associated with Profession Defenders of Wildlife Environmental Defense Fund National Parks Conservation Association National Wildlife Federation Leaders Club Kentucky Goat and Sheep Association Professional Experience July 2014 present: Assistant Professor, Biology Department, Ogden College of Science & Engineering at Western Kentucky University, Bowling Green, KY August 2005 June 2014: Assistant Professor, Liberal Arts & Sciences Department, University College at Western Kentucky University, Bowling Green, KY August : President of Animal Immunodiagnostics, Reagents & Research, LLC Brownsville, KY May August 2006: Research Scientist I and part-time instructor, Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS

3 May May 2001: Postdoctoral Research Associate, Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS June - Aug and 1996: Instructor, Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS June, May 1998: Instructor, Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS Aug. 1995: Graduate assistant, The University of Southern Mississippi's Center for Molecular and Cellular Biosciences' Undergraduate Faculty Enhancement Workshop #4: Protein Chemistry and Immunochemistry. Funded by National Science Foundation's Undergraduate Faculty Enhancement Program : Teaching assistant, Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS Courses Taught Biology for non-biology majors - lecture and laboratory Introduction to Biology (regular and honors) - lecture and laboratory General Microbiology - lecture and laboratory Immunology and Serology - lecture and laboratory Immunochemistry - lecture and laboratory Additional Teaching not in formal course Research methods both in the laboratory and in the literature were taught to undergraduate students both at Western Kentucky University and University of Southern Mississippi. At USM there was a course that students could sign up for credit for this work. At WKU there was not such a course; however, eight separate students have performed this work without receiving academic credit (simply to have the experience). Western Kentucky University (UC) and College Committees (CC) or University Organizations and College Organizations (UO or CO) Advising Task Force Committee (CC) to 2011 Christian Faculty and Staff Fellowship (UO) to 2008 Community College 20 th Birthday Party Planning Committee - January to August 2006 Faculty Search Committee (CC) Geography position - fall 2005 and spring 2006 History position - spring 2007 Research & Creative Activities Program (UC) - college alt ( ), full member ( ) Science Awareness Society (CO) - co-chair 2005 to 2011 Student Travel Award Committee (CC) to 2011 Liberal Arts and Sciences Division Travel Award Committee (CC) to 2011

4 Student Engagement Expo (CC) - co-chair 2008 to 2010 Bowling Green Community College Planning Council (CC) - member 2007 to 2009 Cornerstone Committee (CC) - member 2012 Search Committee for Director, Student Support Programs (CC) - member July 2013 to present South Campus Academic Network Committee (CC) - member 2012 to present University College Sabbatical Leave Committee (CC) member October to November 2012 Continuance Committee for Dr. James Asare (CC) chairperson September 2013 University Southern Mississippi Committees Member of Institutional Animal Care and Use Committee Member of two ad hoc committees responsible for rewriting the "Standard Operating Procedures for the Animal Research Facility" and writing a facility improvement grant Associate member of graduate faculty Served as member on five doctoral and two master's committees Mentor for the McNair Scholars Program Advisor for the Presbyterian Fellowship group Professional Services Reviewer, Journal Article. Aquatic Mammals Drs. Kathleen M. Dudzinski, Justin Gregg, and Robin Paulos (managing editors) asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer, Journal Article. Aquatic Mammals Drs. Kathleen M. Dudzinski, Justin Gregg, and Robin Paulos (managing editors) asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer of Conference Abstracts and Session Chair. International Association for Aquatic Animal Medicine The abstracts that were submitted for the immunology sessions were forwarded from the program chair to me. My job was to review and edit the abstracts for content and format. When I was finished I returned them to the program chair for submission in the conference proceedings. During the conference I was in charge of the session: introducing the talks and keeping the sessions running on time. Reviewer, Journal Article. Aquatic Mammals Dr. Jeanette Thomas, managing editor, asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer, Journal Articles. Developmental and Comparative Immunology

5 Dr. Gregory W. Warr, Co-Editor-in-Chief of the journal Developmental & Comparative Immunology, asked me to review four different article submissions. This journal is an internationally known journal within the field. Book Chapters Middlebrooks, B. L. and R. A. Patterson. August, Methods for purification and study of cetacean immunoglobulins. In Cell and molecular biology of marine mammals. C. J. Pfeiffer ed. Krieger Publishing Co., Inc. Patterson, R. A., B. L. Middlebrooks, and J. C. Jones. August, Application of ELISA methodology for detection of Erysipelothrix rhusiopathiae antibody titers in cetaceans. In Cell and molecular biology of marine mammals. C. J. Pfeiffer ed. Krieger Publishing Co., Inc. Dissertation Patterson, R.A An analysis of the humoral immune system of three species of cetaceans: The Atlantic bottlenose dolphin (Tursiops truncatus), beluga whale (Delphinapterus leucas), and Pacific white-sided dolphin (Lagenorhynchus obliquidens). The University of Southern Mississippi, Hattiesburg, MS. Refereed Publication Heise, R.J., Bringolf, R.B., Patterson, R.A., Cope, W.G., Ross, S.T. (2009). Plasma vitellogenin and estradiol of adult Gulf sturgeon (Acipenser oxyrinchus desotoi) in the Pascagoula River Basin (MS, USA). Transactions of the American Fisheries Society, 138: Jones, J. C., R. A. Patterson, and B. L. Middlebrooks Evaluation and refinement of an ELISA assay designed to detect antibodies against Erysipelothrix rhusiopathiae in cetaceans. Infectious Disease Review. 2(4): R.A. Patterson Congress Report International Workshop on Erysipelas in Cetaceans. Infectious Disease Review. 2(4): Non-refereed Publications Patterson, R. A., J. C. Jones, and B. L. Middlebrooks Development of ELISA methodologies to evaluate anti-erysipelothrix rhusiopathiae titers in several cetacean species. IAAAM News 26(3):6-7. Published Abstracts and Presentations Patterson, R. A. Students Use Classroom Topics to Produce Brochures with International Emphasis. Engaging the Spirit: Celebrating Student Success. WKU, Bowling Green, KY. August 18, Patterson, R. A. Mentoring Students and Colleagues: Step Up and Reach Out Annual Conference of the Kentucky Chapter of the American Association for Women in

6 Community Colleges, Kentucky Chapter of the American Association for Women in Community Colleges, Lexington, KY. February 28, Jensson, Laura and Rhonda Patterson. Note Taking and Test Studying Tips seminar given at the Bowling Green Community College of Western Kentucky University, Bowling Green, KY. Sept, 2006; Feb, 2007; Sept, 2007; Feb, 2008; Sept, 2008; Feb, 2009; Sept, 2010; and Mar, Patterson, Rhonda. Immunology of Marine Mammals: What Do We Know?, invited speaker at the John G. Shedd Aquarium, Chicago, IL. August 8, Coleman, Amaziah, Bobby Middlebrooks, and Rhonda Patterson. Antibody Titers in Four Different Populations of Atlantic Bottlenose Dolphins (Tursiops truncatus) Serum Samples to Erysipelothrix rhusiopathiae. Proceedings of the 37th Annual Conference of the International Association for Aquatic Animal Medicine, 37:86, Boehm, Jeffrey and Rhonda Patterson. Erysipelas in Cetaceans: A Coordinated Research Program. Proceedings of the 16th Biennial Conference of the Society for Marine Mammalogy, p38, Estepp, Jessica D., Bobby L. Middlebrooks, and Rhonda A. Patterson. Development of an Immunodiagnostic Assay for Brucella sp. in the Harp Seal (Phoca groenlandica) and Hooded Seal (Cystophora cristata). Proceedings of the 36th Annual Conference of the International Association for Aquatic Animal Medicine, 36:97, Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. Development of an Enzyme-linked Immunosorbant Assay (ELISA) Using Anti-human IgA Reagents to Determine IgA Levels in the Serum of Several Individuals of the Harbor Seal, Phoca vitulina. Proceedings of the 36th Annual Conference of the International Association for Aquatic Animal Medicine, 36:96, Colvocoresses, Jennifer A., Bobby L. Middlebrooks, and Rhonda A. Patterson. Analysis of Steller Sea Lion IgG and IgM Levels Across the Geographic Range of the Population. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:99, Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. An Investigation of the Cross Reactivity of Antibodies Against the Immunoglobulins of Several Mammalian Species with Immunoglobulins of the Harbor Seal, Phoca vitulina. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35: , Frank, Jill M., Bobby L. Middlebrooks, and Rhonda A. Patterson. Characterization of Immunoglobulin G (IgG) in the Spectacled Eider, Somateria fischeri. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:133, 2004.

7 Patterson, Rhonda A., Nicole A. Lilly, and Bobby L. Middlebrooks. IgG and IgA Concentrations Determined in Serum and Milk from Atlantic Bottlenose Dolphins (Tursiops truncatus) with Monoclonal Antibodies Specific for the Immunoglobulin Isotypes. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:134, Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. Preliminary study of the isolation and characterization of the immunoglobulins of the harbor seal, Phoca vitulina. Proceedings of the 34th Annual Conference of the International Association for Aquatic Animal Medicine, 34:70, Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Cloning and expression of the gene sequence of a 66kD surface protein of Erysipelothrix rhusiopathiae. Proceedings of the 33rd Annual Conference of the International Association for Aquatic Animal Medicine, 33:7, Lilly, Nicole A., Rhonda A. Patterson, and Bobby L. Middlebrooks. Development of a monoclonal antibody against Tursiops truncatus IgG for use as a reagent in a diagnostic ELISA. Proceedings of the 33rd Annual Conference of the International Association for Aquatic Animal Medicine, 33:8, Colvocoresses, Jennifer, Bobby Middlebrooks, and Rhonda Patterson. Evaluation of Canine and Human Antisera as Diagnostic Reagents for Use in the Identification of Immunoglobulins of Steller Sea Lions (Eumetopias jubatus) and Harbor Seals (Phoca vitulina). Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:94, Thames, Rachel, Jennifer Woodford, Rhonda Patterson, Frank Moore, Stan Kuczai, and M.A. Solangi. Effects of Illness, Associated Medicines, Age and Time of Day on Diurnal and Nocturnal Behavior of Captive Bottlenose Dolphins (Tursiops truncatus). Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:98, Boehm, Jeffrey, R., Geraldine Lacave, and Rhonda Patterson. Advancing the Study and Control of Erysipelas in Cetaceans. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32: , Jones, John C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Comparison of a Developmental ELISA for Anti-Erysipelothrix rhusiopathiae Antibodies with a Passive Agglutination Assay Using Latex Beads Coated with a Purified 64 kda E. rhusiopathiae Specific Protein. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:108, Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. The Use of Molecular, Immunological and Microbiological Methods to Evaluate Similarities and Differences Between ATCC Reference Strains of Erysipelothrix rhusiopathiae and

8 Erysipelothrix tonsillarum and Environmental Isolates of Erysipelothrix rhusiopathiae Implicated in the Death of Marine Mammals. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:109, Patterson, Rhonda A. What's New in Erysipelothrix rhusiopathiae Research in Marine Mammals? Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 210, Colvocoresses, Jennifer, Rhonda A. Patterson, and Bobby L. Middlebrooks. Purification of Immunoglobulin Isotypes G, A, and M from the Serum of the Steller Sea Lion (Eumetopias jubatus). Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 477, Jones, John C., Rhonda A. Patterson, and Bobby L. Middlebrooks. Use of a Mouse Model to Determine the Specificity of an ELISA Assay Designed to Detect Antibodies Against Erysipelothrix rhusiopathiae in Cetaceans. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 511, Middlebrooks, Bobby, L., Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Four Developmental Cell Lines from the Beluga Whale (Delphinapterus leucas) and the Pantropical Spotted Dolphin (Stenella attentuata) to Seal Herpes Virus and a Range of Mammalian Viruses from Several Families. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 526, Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Characterization and Comparison of Five Isolates of Ersyipelothrix from Several Marine Mammal Sources. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 541, Patterson, Rhonda, and Bobby Middlebrooks. Preparation of Antisera Specific for Major Immunoglobulin Isotypes (IgG, IgA, and IgM) of Three Species of Cetacean: Atlantic Bottlenose Dolphin (Tursiops truncatus), Pacific White-Sided Dolphin (Lagenorhynchus obliquidens), and the Beluga Whale (Delphinapterus leucas). Proceedings of the 13th Biennial Conference on the Biology of Marine Mammals, p 123, Middlebrooks, Bobby, Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Two Developmental Cell Lines from Tursiops truncatus to a Range of Mammalian Viruses. Proceedings of the 13th Biennial Conference on the Biology of Marine Mammals, p124, 1999.

9 Patterson, Rhonda. What Do We Know About the Immune Systems of Cetaceans and How Can We Apply It to Studying Steller Sea Lions?, invited speaker at the Alaska SeaLife Center, Seward, Alaska, July 1, Patterson, Rhonda. Dolphin Verses Dog Intelligence, invited speaker for fifth graders at the Dixie Attendance Center, Dixie, Mississippi, March 27, Patterson, Rhonda. Basics of the Immune System, invited speaker for high school advanced placement students at Poplarville High School, Poplarville, Mississippi, September 30, Patterson, Rhonda. Serology: Assays for determination of immunoglobulin levels and for determination of pathogen specific antibody titers, Pre-Conference Marine Mammal Immunology Workshop for Clinicians at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Patterson, Rhonda. Vaccines: Types, routes of exposure, and possible carriers, Pre- Conference Marine Mammal Immunology Workshop for Clinicians at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Jones, John C., Rhonda A. Patterson, and Bobby L. Middlebrooks. The Antigenic Components of a Wild Strain of Erysipleothrix rhusiopathiae Determined by Immunostaining of Extracted Bacterial Surface Components with Serum from Tursiops truncatus, Lagenorhynchus obliquidens, and Delphinapterus leucas. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:20-21, Colvocoresses, Jennifer, Rhonda A. Patterson, and Bobby L. Middlebrooks. Preliminary Evaluation of Viral Susceptibility in Primary Kidney and Lung Cell Cultures of Tursiops truncatus. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:52, Walter, F. Lynn, Rhonda A. Patterson, and Bobby L. Middlebrooks. Preliminary Evaluation of the Kinetics of Changes in Lectin Levels Following Antigen Exposure as a Measure of Potential Disease Resistance in Strains of Penaeus vanammei. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:65-66, Osgood, Robert, Rhonda A. Patterson, and Bobby L. Middlebrooks. Application of Biochemical, Immunochemical, and Molecular Analysis to Comparison of Erysipelothrix rhusiopathiae Isolates from Two Species of Cetaceans to Each Other and to Strains Obtained from the American Type Culture Collection. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:82, 1999.

10 Middlebrooks, Bobby, Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Two Developmental Cell Lines from Tursiops truncatus to a Range of Mammalian Viruses. Abstracts of the 13th Biennial Conference on the Biology of Marine Mammals, p124, Patterson, Rhonda, and Bobby Middlebrooks. Preparation of Antisera Specific for Major Immunoglobulin Isotypes (IgG, IgA, and IgM) of Three Species of Cetacean: Atlantic Bottlenose Dolphin (Tursiops truncatus), Pacific White-Sided Dolphin (Lagenorhynchus obliquidens), and the Beluga Whale (Delphinapterus leucas). Abstracts of the 13th Biennial Conference on the Biology of Marine Mammals, p146, Patterson, Rhonda. What Do We Know About the Immune Systems of Dolphins and Whales?. Presented at the Biological Sciences Departmental Seminar Series, March 19, Patterson, R. A. and B. L. Middlebrooks The jacalin affinity chromatography column proves to be an effective method for purifying IgA from Atlantic bottlenose dolphin (Tursiops truncatus), beluga whale, (Delphinapterus leucas), and Pacific whitesided dolphin (Lagenorhynchus obliquidens) serum. p In Proceedings of the 29th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A. and B. L. Middlebrooks Evidence of immunoglobulins (IgG) in the milk of two cetacean species, Tursiops truncatus and Lagenorhynchus obliquidens. p. 78. In Proceedings of the 28th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A., J. C. Jones, and B. L. Middlebrooks Comparison of species specific antisera and cross-reactive antisera in an ELISA for the determination of serum antibody titers against Erysipelothrix rhusiopathiae in Delphinapterus leucas and Lagenorhynchus obliquidens. p.79. In Proceedings of the 28th Annual International Association of Aquatic Animal Medicine Conference. Jones, J. C., R. A. Patterson, and B. L. Middlebrooks Comparison of ELISA and agglutination titers against Erysipelothrix rhusiopathiae in cetaceans. p. 52. In Proceedings of the 27th Annual International Association of Aquatic Animal Medicine Conference. Jones, J. C., R. A. Patterson, and B. L. Middlebrooks A biotin-avidin amplified ELISA for the detection of antibodies against Erysipelothrix rhusiopathiae in captive dolphins. p. 54. In Proceedings of the 26th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A. and B. L. Middlebrooks Establishment of primary tissue culture lines of tissues from an Atlantic bottlenose dolphin (Tursiops truncatus) fetus. p. 55. In Proceedings of the 26th Annual International Association of Aquatic Animal Medicine Conference.

11 Patterson, R. A., B. L. Middlebrooks, and M. A. Solangi Effect of immunization schedule on immunoglobulin response in the Atlantic bottlenose dolphin (Tursiops truncatus). p. 1. In Proceedings of the 22nd Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A Purification of immunoglobulins (IgG, IgA, and IgM) in the bottlenose dolphin (Tursiops truncatus). p In Proceedings of the 21st Annual International Association of Aquatic Animal Medicine Conference. Funded Research Proposals Title: Junior Faculty Scholarship Award Agency: WKU Office of Sponsored Programs Funds: $4000 (June 2007 to May 2008) Role: Responsible person for funds Title: Teaching students about environmental sustainability and civic responsibility through plastic recycling at south campus Agency: WKU Provost (Provost Initiative in Excellence - PIE grant) Funds: $1333 (March to December 2007) Role: Responsible person for funds Title: Erysipelas in Cetaceans: A Coordinated Research Program Agency: The John G. Shedd Aquarium Funds: $20,000 (January to June 2007) Role: Principal Investigator Title: Evaluation of humoral immune system parameters in Steller sea lions: preliminary evaluation of immunoglobulin isotypes found in low concentrations and continued reagent development Agency: Alaska SeaLife Center Funds: $30,000 (October 2004 to September 2005) Title: Production of a DNA Vaccine Specific for the 64 kd Protective Antigen of Erysipelothrix rhusiopathiae Agency: Office of Naval Research Funds: $142,739 (ending August 2005) Title: Study to measure baseline levels of immunoglobulin classes in captive dolphins Agency: U.S. Department of Commerce/NMFS Institute for Marine Mammal Studies Funds: $74,969 (ended November 2003)

12 Title: Investigation of the role of immunoglobulin classes and subclasses in humoral and mucosal immunity in cetaceans Agency: Office of Naval Research Funds: $397,054 (ended June 2005) Title: Development and Application of Reagents and Methodologies for Analysis of Comparative Immunoglobulin Levels in Harbor Seals. Agency: Alaska SeaLife Center Funds: $20,000 (ended September 2002) Title: Development and Evaluation of Monoclonal Antibodies for Analysis of Immunoglobulin Isotypes in Eiders (Including Spectacled and Steller Eiders) Agency: Alaska SeaLife Center Funds: $10,000 (ended September 2002) Title: Immunoglobulin Isotype Levels in Steller Sea Lions. Agency: Alaska SeaLife Center Funds: $10,000 (ended September 2002) Title: Preliminary study to determine the effectiveness of intramuscular, oral, and respiratory immunizations in producing immunogen specific antibodies in the Atlantic bottlenose dolphin (Tursiops truncatus) Agency: Anheuser-Busch Sea World Parks Funds: $30,000 (ended August 2005)