School Of Medicine Biomedical Sciences

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1 School Of Medicine Biomedical Sciences COSHH Risk Assessment Form Before any experimental procedure involving the use of chemicals or reagents is undertaken, potential hazards must be identified and an assessment made of the risks posed by these hazards in the work activity in which they are involved. This risk assessment is a legal requirement to comply with the Control of Substances Hazardous to Health (CoSHH) Act and is the responsibility of the research workers supervising the research projects. Before undertaking experimental procedures, all members of staff must read and sign the relevant CoSHH assessments Title of Procedure: Labs Involved: Brief Description: Staining of tissue sections with histological dyes EU33, DU14, SITraN Demonstration of various tissue components with a range of histological dye techniques Please note: Known carcinogens, teratogens and mutagens should only be used in consultation with senior staff and the Departmental Safety Officer. All user must have read University supplementary Code of Practice for the use of such substances and comply with its recommendations. It is important to realise that the use of certain carcinogens is banned by law. Chemical/Reagent Hazard Stock Volume/ Quantity Risk level Precautions Disposal Ammonium (para)tungstate hydrate Irritating to eyes, respiratory system and skin. 100g Medium GLP. Handle in fume. Chemical waste stream Acetic acid 1,1.2, 2, 2.5 and 100% Flammable. Causes severe burns 2ml Low GLP. Use in Fume. Acetone Highly flammable.vapour narcotic in high concentration. Avoid contact with eyes 50ml Low GLP, use in Solvent drum Y Acid Fuchsin 1% Causes cancer in lab animals 15ml Low GLP Alizarin Red S May cause irritation Aluminium sulphate Harmful by inhalation skin and eye contact 5g Low GLP. Wear goggles amounts of

2 Ammonia + Ammonia hydroxide Causes burns, severe irritant to eyes, skin, respiratory tract 100ml Low GLP, use in. Ammonium ferric sulphate May irritate eyes and skin Ammonium oxalate Irritation to skin and respiratory tract 0.8g Low GLP Dissolve in excess and flush down sink Celestine blue B Combustible 0.5g Low GLP Dissolve in excess and flush down sink Carbol Fuchsin solution Crystal ponceau 1% crystal violet Flammable. Harmful in contact with skin and if swallowed may cause irritation to eyes and harmful by ingestion may be carcinogenic. Risk of serious damage to eyes 6ml Low GLP 2g Low GLP Wear goggles Dissolve in excess and flush down sink Dextrin 1% none 4ml Low GLP Ethanol 70,95 and 100% ferric chloride 4% Formaldehyde Conc and 10% Giemsa solution highly flammable. Irritating to eyes. Intoxicating if inhaled or ingested potentially carcinogenic. Harmful by inhalation, skin contact, swallowing Harmful by inhalation, skin contact, swallowing. Flammable and potentially carcinogenic Highly flammable toxic by inhalation, skin contact and swallowing 50ml Low GLP 10ml Low GLP 4ml Low GLP Glycerol none 14ml Low GLP Gold chloride 0.2% and 0.1% Haematoxylin Hexamine 3% Causes burns, may cause skin sensitisation potentially carcinogenic, combustible Highly flammable. May cause sensitisation by skin contact and inhalation 0.5g Low GLP 100ml Low GLP

3 Hydrochloric acid conc and 2% Causes burns 5ml Low GLP, use conc acid in Iodine Light green Lithium carbonate Martius yellow 0.5% Harmful by inhalation and by skin contact May cause irritation to skin, eyes and mucos membranes Harmful if swallowed, irritating to eyes Combustable. May be harmful to skin, eyes etc 1g Low GLP,use in In solution,down amounts of 1g Low GLP May Grunwald solution harmful to skin, eyes etc Metanil yellow Risk of serious damge to eyes potentially carcinogenic 0.25g Low GLP In solution,down amounts of Methyl blue 0.5% May be harmful Methyl green 2% Not known 9ml Low GLP Millers elastin Highly flammable. May be harmful after inhalation 20ml Low GLP Nitric Acid Concentrated Cause severe burns 1 drop Low GLP, use in Oil red O Avoid contact with eyes and skin 1g Low GLP Orange G 2% None Oxalic acid 1% Periodic acid 1% Irritant and caustic effects. Evidence of reproductive deffects Contact with combustible material may cause fire. Irritant to respiaratory tract Phosphomolybidic acid Causes burns

4 Phosphotungstic acid causes burns Picric acid Ponceau de Xylidine (Ponceau 2R) Potassium ferrocyanide Causes dermatitis. May be harmful by inhalation. Evidence of mutagenic effects. Explosive if dry. Toxic by inhalation, skin contact and swallowing. Harmful. Possible realse of hydrocyanic acid 90ml Low GLP. Always keep powder covered with a copious volume of. 30ml Low GLP, use in copious aamounts of 5ml Low GLP Potassium hydroxide 10% Causes severe burns 1ml Low GLP Potassium metabisulphate 3% Potassium permanganate Propan-2-ol contact with acids liberates toxic gas. Irritating to respiratory tract may irritate skin, eyes and mucous membranes Highly flammable. Irritating to eyes. Vapour may cause drowsiness 2ml Low GLP. Avoid contact with acid Pyronin none 4ml Low GLP Schiff reagent irritating to respiratory tract Silver nitrate causes burns. May ignite combustible material. Irritant to eyes, skin 40ml Low GLP Sirius red Combustible. May cause irritation 0.5g Low GLP sodium acetate none 30ml Low GLP Sodium Chloride 20% none 4ml Low GLP Sodium hydroxide irritant and caustic effects after skin and eye and inhalation 5ml Low GLP Sodium tetraborate 5% danger of skin absorption

5 Toludine blue combustible Nuclear fast red Safranin O Fast Green FCF Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation. Causes serious eye damage Irritant. Suspected of causing genetic defects. Carcinogenic. 5ml Low GLP. Use in fume. >5ml Low GLP Use in fume. 0.01g Low GLP In solution,down amounts of Azure A eosinate Methylene Blue Methylene Violet Tetrachrome stain Methanol May cause irritation. May be harmful by inhalation, ingestion or skin absorption. May cause irritation. May be harmful by inhalation, ingestion or skin absorption. May cause irritation. May be harmful by inhalation, ingestion or skin absorption. May cause irritation. May be harmful by inhalation, ingestion or skin absorption. Highly flammable. Toxic by inhalation & ingestion. 0.8g Low GLP In solution,down amounts of. 0.5g Low GLP In solution,down amounts of. 0.1g Low GLP amounts of. 0.1g Low GLP amounts of. 250ml Low GLP Category Y waste drum. Small quantities diown amounts of Gills Haematoxylin See COSHH ml Low GLP Transfer to waste solvent drum Y Sodium thiosulphate 2.5g Low GLP Sodium tartrate Not hazardous. 10g Low GLP In solution,down amounts of Naphthol AS-BI phosphate May irritate skin, eyes and mucous membranes. 40mg Low GLP Hazardous waste stream Dimethylformamide Sodium nitrite Do not use when pregnant. Harmful by inhalation & in contact with skin. Irritating to eyes. Toxic if swallowed. Contact with combustible material may cause fire. 2ml Medium GLP. Wear double gloves and use in fume. Hazardous waste stream 160mg Medium GLP in solution,down amounts of

6 Xylene Flammable. Irritant.TWA 50ppm 2.5L Low GLP. Use in.don't use near heat source or naked flame Waste solvent drum Y Calcium nitrate Irritant 100g Low GLP In solution downsink with copiuos amounts of Alcian blue Combustible <1g Low GLP amounts of Pyronin Y Harmful. Possible mutagen <1g Low GLP amounts of Cobalt Chloride lead nitrate Pontamine Sky Blue.5BX Sodium Iodate Harmful. Reacts violently with alkali metals Harmful. Toxic. Major effects due to accumulation of lead. Combustible.Harmful. Irritant.Can react with oxidising materials. Irritant. May ignite combustible material <1g Low GLP use in fume amounts of <5g Low GLP. Use in fume. <5g Low GLP. Use only in fume Via Health and Safety chemical collection amounts of <5g Low GLP.Use in fume amounts of Malachite Green Combustible <1g Low GLP. Use in fume. amount of Erichrome Black T Harmful. Irritant <1g Low GLP amounts of Potassium Iodate Harmful. Irritant.Combustible <5g Low GLP. Use in fume. amounts of Ammonium potassium sulphate Irritant <100g Low GLP amounts of. Tris maleate None 2.37g Low GLP. Avoid inhalation. Magnesium chloride None 1g Low GLP. Avoid inhalation. Fast Red Violet LB onium salt Harmful by inhalation, in contact with skin and if swallowed. Limited evidence of a carcinogenic effect. 0.14g Low GLP. Handle in fume Naphthol AS-MX phosphate Toxic 0.2mg Low GLP. Handle in fume In solution,down amounts of In solution,down amounts of. amounts of amounts of

7 Fast Blue BB salt Combustible. Harmful. Irritant. 0.4mg Low GLP. Handle in fume amounts of Catalase None 1g Low GLP amounts of. Cytochrome C None 50mg Low GLP amounts of. Disodium succinate hexahydrate Irritant to skin, eyes and respiratory 100g Medium GLP Handle in fume Chromium trioxide/chromic acid 5% Congo Red Van Gieson Pyrogallol Flammable, May cause cancer, toxic by inhalation and skin contact, causes severe burns Reproductive Toxicity, Carcinogenic, Eye irritant, Flammable Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Germ cell mutagenicity (Category 2) Acute toxicity, Inhalation (Category 50g powder, 100ml 5% chromic acid High GLP. Wear safety specs when weighing powder. Keep away from heat and sources of ignition 10g High wear facemask and weigh out in a fume, wear lab coat, gloves and safety glasses, don't use if pregnant 500ml Low wear lab coat, gloves and safety glasses 100g High Wear lab coat, gloves and face mask when weighing Citric Acid Eye irritant 500g Low Wear lab coat and gloves Cresyl Violet acetate Luxol fast blue powder. Congo Red Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008 Causes serious eye irritation. May cause cancer. Suspected of damaging the unborn child. 10g Low Wear lab coat and gloves 100g Low Wear lab coat and gloves 100g High wear facemask and weigh out in a fume, wear lab coat, gloves and safety glasses, don't use if pregnant Hazardous waste stream dilute to less that 0.1% and flush down sink with. down the sink with down the sink with Dispose via Safety Services (concentration for Bielschowsky, Modified, can go down the sink with ) Wash down the amounts of Wash down the amounts of Wash down the amounts of down the sink with

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9 Good Laboratory Practice should be adhered at all times Common sense at all times! Never eat, drink or apply cosmetics in the laboratory. Wash your hands in the designated sinks whenever you have finished your lab work. Be familiar with the potential hazards involved in your work and take appropriate precautions. Wear protective clothing as needed, lab coats are a minimum. Keep your work area clean and tidy. Dispose of materials appropriately. Use appropriate care with electrical equipment. Use appropriate care with sharps Use appropriate care with substances of extreme temperatures (liquid nitrogen or hot liquids) Operate maintain and service all your equipment properly, if in doubt ask! Work in appropriate containment s where necessary. Precautions to be taken: Use in fume : acetic acid, ammonia, periodic acid, nitric acid, Formaldehyde, HCL, acetone & dimethylformamide. Wear gloves and mask when weighing out dyes. Wear gloves when performing staining procedures. Picric acid should never be allowed to dry out as it may become explosive EMERGENCY PROCEDURES FIRST AID IF PERSONNEL ARE AFFECTED (fumes, contamination/contact) TREATMENT TO BE ADOPTED EYES Irrigate with eye wash for at least 10 minutes. Obtain medical attention. LUNGS Remove from exposure. Rest and keep warm. In severe cases obtain medical attention. SKIN Drench skin with. Remove contaminated clothing. In severe cases seek medical attention. MOUTH Wash mouth out thoroughly and give very small SIPS of. Do not induce vomiting. Obtain medical attention. Special Precautions: Formaldehyde spillages : appropriate protective clothing should be used (gloves, face mask etc). Ensure adequate ventilation Assessed by: Y. Stephenson Date: 19/04/2013 Approved by Y Stephenson Date approved: 22/04/2013