School Of Medicine Biomedical Sciences
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1 School Of Medicine Biomedical Sciences COSHH Risk Assessment Form Before any experimental involving the use of chemicals or reagents is undertaken, potential hazards must be identified and an assessment made of the risks posed by these hazards in the work activity in which they are involved. This risk assessment is a legal requirement to comply with the Control of Substances Hazardous to Health (CoSHH) Act and is the responsibility of the research workers supervising the research projects. Before undertaking experimental s, all members of staff must read and sign the relevant CoSHH assessments Title of Procedure: Labs Involved: Brief Description: Analysis of compounds on LC-MS M147 Solvent extraction of pharmacological compounds and their metabolites from biological samples (urine, plasma, human liver microsomes and recombinant microsomes) and subsequent analysis by LC-MS. See CoSHH form no 174 for making up of solutions used in this. Please note: Known carcinogens, teratogens and mutagens should only be used in consultation with senior staff and the Departmental Safety Officer. All user must have read University supplementary Code of Practice for the use of such substances and comply with its recommendations. It is important to realise that the use of certain carcinogens is banned by law. Chemical/Reagent Hazard Stock Volume/ Quantity Emergency Action Risk level Precautions Disposal Methanol 5-70% Highly flammable, toxic by ingetsion, inhalation and skin. Chronic effect on eyes. Longterm wear eye
2 Acetonitrile 5-70% flammable, toxic by inhalation and -CYANIDE COMPOUND Long term wear eye Hexane 5-70% Flammable, toxic by inhalation, Ingestion, Irritant on eye and long term wear eye Propan-2-ol 5-70% Flammable, toxic by inhalation ingestion, irritant on eye and skin contact, long term WEL=400ppm, wear eye Methyl-tert-Butyl Ether. Flammable, toxic by inhalation ingestion, irritant on eye and skin contact long term WEL=25 ppm, n case of fume cupboard wear eye
3 Ethanol Highly flammable, vapour/air mix Explosive. Irritant to eye, skin and Digestive tract. Long term fume cupboard wear eye 5-20mM Ammonium Acetate or acetate Buffer None of in black bin bag Low None Glycine Buffer None <200ml of in black bin bag Low None Down sink M Sodium Hydroxide (NaOH) Toxic on ingestion Severe irritant as dust Corrosive. Short term WEL=2mg.m3 <100ml roll wearing unpowdered vinyl or nitrile dispose Down sink Trifluroacetic Acid (TFA) Toxic by inhalation, causes severe burns, corrosive <100ml roll wearing unpowdered vinyl or nitrile Down sink Perchloric Acid (PCA) 60% Toxic by inhalation, causes severe burns, corrosive <100ml roll wearing unpowdered vinyl or nitrile. Avoid using in fume hood. Down sink Biological sample /incubation mixture Viral and bacteriological <1ml/sample Cover in 10%virusolve leave 30min and roll put in yellow bin Tropisitron/6 OH Ondansitron none at concs used l Testosterone And associated Metabolites R+S Methadone l LOW-HIGH LOW. All users to be vaccinated against Hepititis B and had their antibody levels checked Yellow bin, orange lid
4 Nicotine, Cotinine Nicotine methyl D3 Cotinine D3 Clomiphene isomers Didesmethyl tamoxifen Codeine, morphine Norcodiene and 3OH morphinan Prazepam/Midazolam/1OH Midazolam Amitriptyline Nortriptyline and their associated metabolites <100ml of l Zuclopenthixol/Flupenthixol <100ml of l Metoprolol <100ml of l Low Compressed Nitrogen asphyxiant! Large compressed cylinder immediately evacuate room if O2 depletion meter sounds and inform floor leader High wear impact level 1 eye, all users should have attended central workshops gas safety course O2 depletion meter should be installed in laboratory cylinder returned to pharmacy Diethylamine Flammable, toxic by ingestion Inhalation and skin contact Long term WEL= 5ppm, Short term WEL 500ml Cover in absorbent material found in M105, place in fume hood to evaporate and dispose of via hospital waste route when totally evaporated fume hood.ensure hood working before commencing using vanometer wear eye and upowdered nitrile. Rifampicin Harmful if swallowed. Irritating to eyes, respiratory system and skin. 100ml of l Low
5 Rifamycin SV Not hazardours according to directive 67/548/EEC 100ml l None Low None
6 Good Laboratory Practice should be adhered at all times Common sense at all times! Never eat, drink or apply cosmetics in the laboratory. Wash your hands in the designated sinks whenever you have finished your lab work. Be familiar with the potential hazards involved in your work and take appropriate precautions. Wear protective clothing as needed, lab coats are a minimum. Keep your work area clean and tidy. Dispose of materials appropriately. Use appropriate care with electrical equipment. Use appropriate care with sharps Use appropriate care with substances of extreme temperatures (liquid nitrogen or hot liquids) Operate maintain and service all your equipment properly, if in doubt ask! Work in appropriate containment hoods where necessary. Precautions to be taken: SOPs for centrifuges, multi-tube vortexers, mass spec, vortex evaporators to be read before commencing. Solvent and Acid winchesters (2.5L) to be carried in winchester carriers when they are being moved between labs EMERGENCY PROCEDURES FIRST AID IF PERSONNEL ARE AFFECTED (fumes, contamination/contact) TREATMENT TO BE ADOPTED EYES Irrigate with eye wash for at least 10 minutes. Obtain medical attention. LUNGS Remove from exposure. Rest and keep warm. In severe cases obtain medical attention. SKIN Drench skin with Remove contaminated clothing. In severe cases seek medical attention. MOUTH Wash mouth out thoroughly and give very small SIPS of Do not induce vomiting. Obtain medical attention. Special Precautions: Assessed by: A.Gregory Date: 07/09/2007 Approved by Date approved:
School Of Medicine Biomedical Sciences
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