GRAC - Perchlorate in Groundwater. Enhancement of Perchlorate Analysis using Solid Phase Extraction (SPE) Cartridges
|
|
- Martha Carter
- 6 years ago
- Views:
Transcription
1 Enhancement of Perchlorate Analysis using Solid Phase Extraction (SPE) Cartridges Victor F. Medina*, Steven L. Larson, Barbara Extine United States Army Corp of Engineers Engineer Research and Development Center Vickburg MS. * victor.f.medina@erdc.usace.army.mil, (601) Introduction Perchlorate is a contaminant of increasing concern at U.S. Army facilities - primarily from weapons production and firing of solid fuel rockets. Perchlorate has a high solubility (about 200 g/l) and is resistant to degradation (Espenson 2000). The Engineer Research & Development Center (ERDC) is conducting studies on biodegradation of perchlorate. Although methods using technologies such as electron spray mass spectroscopy have been presented in the literature (Magnuson et al. 2000), analysis of perchlorate is typically performed using ion chromatography. Perchlorate treatment levels are in the low ppb range (the CADHS action level is 4 ug/l). Large injection loop injection (with injection loops of 0.5 to 1 ml) has been demonstrated to be effective for perchlorate analysis down to these low concentration levels (Dionex, undated; CADHS 1997; Hautman et al. 1999; Ellington and Evans 2000; Jackson et al 2000; Tsui et al. 2000; Collette et al. 2001). However, this method is not convenient for our needs. To begin with the initial concentrations of our samples are in the range of 20 to 40 mg/l. We would have to change the configuration of the instrument for initial and final concentration measurements, or use extensive dilution. In addition, our instrument is not available for dedicated use for the project. It is extensively used for analysis of chloride, phosphate, nitrate, and other anions, supporting 4 other projects. Development of another, reliable method to reach low concentration levels without requiring a change in our current operating configuration would be of a great value to us. Solid phase extraction (SPE) uses columns of sorbent material in which samples are filtered. The material removes the contaminant of interest by physico-chemical means. The contaminant is then extracted in a smaller volume using an appropriate solution, thereby concentrating the contaminant. SPE is commonly used for the analysis of low levels of pesticides (Manuel 2000) and munitions (Jenkins et al. 1995; Larson et al. 2002). Perchlorate exists as an anion. Anionic SPE filters exist and are commonly used for compounds such as arsenate. The removal process is ion exchange. Ion exchange has been extensively studied as a treatment technology, and these provide key background for SPE application (Batista et al. 2000, 2002; Gingas and Batista 2002; Viera 2000). Generally, these studies indicate that perchlorate is readily exchanged. There are some resins that perform very poorly, but with the right ion exchange materials, it is possible to achieve about 90% removal or higher. However, a challenge in ion exchange treatment of perchlorate is that, for some materials, the sorbed pechlorate is not recoverable by 1
2 regeneration. Strong base anion exchange resins generally remove high levels of perchlorate, but tend to be resistant to regeneration. Weak base anion exchange resins may not remove perchlorate effectively. However, some resins, both strong and weak base, remove high levels of perchlorate and are allow for high recovery of the perchlorate. These findings indicate that resin selection was likely to be an important parameter SPE filtration. This paper investigates the use of anionic solid phase extraction cartridges to concentrate perchlorate, improving its detection in our existing IC configuration. Preliminary results detailing its effectiveness are presented. Methods SPE Filtration & Extraction Figure 1 summarizes our basic approach. Large volumes of water can be filtered through the SPE cartridge, concentrating perchlorate solution on the solid media. Then, the perchlorate can be recovered using a regenerant. Figure 1. Basic Method for SPE Filtration of Perchlorate Contaminated Water Pretreat Sample as needed (centrifugation, preliminary filtration) Filter the 1-L sample through the NH2 SPE Cartridge. If concerned about breakthrough, take a sample of the last 2 ml passed through the filter. Carefully measure 1-liter of the sample solution Extract & recover sorbed perchlorate with 2 ml of 1% NaOH (12% NaCl used in some experiments) Set up NH2 SPE filter(s) (1.0 ml filter volume) on filtration manifold Analyze extract by Ion Chromatography Pretreat SPE filter: 2 ml methanol followed by 2 ml DI water Calculate solution concentration (mass balance) We chose 1-L as our standard filtration volume because this has been commonly used in other SPE applications (Larson et al. 2002). Filtration of 1-L of relatively clean water can take 100 or more minutes, so although larger volumes could improve detection, it might not be practical from a time sense for large numbers of samples. The filtration velocity for clean water averaged 10 ml/min, and filtration pressure was about 20 psi. The results presented in this paper are for perchlorate solutions in deionized (DI) water. Table 1. Ion Chromatograph Specifications Parameter Name/Setting/Value Instrument Dionex DX 500 Flow Rate 1.5 ml/min Mobile Phase Isocratic: 33.5 mm NaOH Run Time 12 minutes Injection Loop 10 µl Separation Column AS-11 Detector Dionex CD 20 Conductivity Detector Detector Amperage 100 ma Approx. Perchlorate 6.80 minutes Peak Time Filtration times for DI water samples range took 100 to 150 minutes. However, since many samples could be processed simultaneously. Care must be taken to avoid excess spills, pouring samples into the wrong cartridge, identifying leaks, etc. Ion Chromatography Table 1 summarizes key operating parameters for the IC used in this study. Notice that the sample injection loop was 10 ul, which is 100 times smaller than the loop used in USEPA Method 314.0, the large sample injection method for low-level perchlorate analysis. Method Detection Limit (MDL) Method detection limit (MDL) was analyzed and calculated using the approach given in Standard Methods (Greenberg et al. 1992), which is consistent with the approach specified by the Federal Register (1984). This involves collection and analysis of 7 2
3 replicate samples. The standard Table 2. Filter Material and Regenerant Study deviation is calculated from these samples. The MDL is defined as Filtered Filtered Mass Extracted Extracted Filter Concent. Volume removed Concen. Volume Material Extractant (mg/l) (ml) (mg) (mg/l) (ml) the product of the standard deviation and the t-statistic value, which for 7 samples is equal to The Federal Register stipulates that the concentration used for the MDL test must be within a range of 1 to 5 times the calculated MDL. Figure 2. Comparison of Chromatograms of Perchlorate Extracted from an NH2 SPE with 12% NaCl and 1% NaOH. Note Scale Difference. 3,500 PER C H LO R ATE1ppm _N ac l_n H 2_5M M _ N ac l_n H 2filter-125:7 ECD 2,000 1, perchlorate % Sodium Chloride Extraction (perchlorate peak = 146 mg/l) 200 PERC H LO R ATE1PPM _N AO H_N H2_5M M _ N ao H _N H 2filter-125:7 ECD perchlorate % Sodium Hydroxide Extraction (perchlorate peak = 126 mg/l) Results Filter & Regenerant Selection Our study initially focused on selecting a commercially available SPE type, and a regenerant. We tested three commercially available media, NH2, WCX, and PSA. Each media type is available from a variety of suppliers, including Supelco and Varian. 3 ml cartridges were used in these experiments. Each media type proved to be effective at removing perchlorate from solution. Trial and error studies indicated that each filter type could remove about mg of perchlorate before breakthrough, this corresponding to about 200 ml of a 1.34 mg/l solution Two regenerants, 1% NaOH and 12% NaCl, were tested. These choices were based on ion exchange research published by Batista et al. (2002). No perchlorate was recovered off the WCX material (Table 2). 84% of the applied perchlorate was recovered from the PSA material using 2% NaCl, but only 9% with NaOH. >90% recovery was achieved from the NH2 columns with both NaCl and NaOH. Therefore, NH2 was chosed as the SPE material for subsequent work. We chose to focus on NaOH as the regenerant because NaCl creates a large Cl - peak (Figure 2). Although this does not adversely effect perchlorate measurement, it is more likely to interfere with perchlorate transformation products than the 1% NaOH solution. Mass Recovered (mg) Percent recoverey NH2 12% NaCl % NH2 1% NaOH % WCX 12% NaCl % WCX 1% NaOH % PSA 12% NaCl % PSA 1% NaOH % Figure 3. Comparison of analysis of mg/l perchlorate solution, with and without SPE concentration 45.0 PERCHLO RATE_0.1_0.001ppm _NAO H_NH2_ #8 Final0.1ppm _1L_ :7 ECD Chromatogram for mg/l perchlorate. Perchlorate is below instrument detection level PERCHLO RATE_0.1_0.001ppm_NAO H_NH2_ #9 NaO H-1_NH2_1L0.1ppmPerchl25 ECD_ perchlorate Chromatogram for mg/l perchlorate concentrated to 168 mg/l using SPE (1-Liter filtered, extracted by 2 ml NaOH) 3
4 Comparison of Analysis, With & Without SPE Preconcentration Figure 3 compares two chromatograms. The first was a direct injection of a mg/l solution using the configuration given in Table 1. The chromatogram reveals that the concentration was below the instrument detection limit. Below is a chromatogram of the same solution, which was concentrated using an NH2 SPE cartridge. A perchlorate peak was easily identified, as it had a concentration of 168 mg/l. The actual concentration of the solution was calculated by mass balance, in this case it equals mg/l. This represents a recovery of about 93% of perchlorate in the sample. Table 4. MDL Analysis on SPE Filtration Method Specified in Methods Section (NH2 Cartridge, 60 ug/l, 1-Liter Volume, Extraction with 2-ml of 1% NaOH) Recovery Versus Table 3 summarizes perchlorate recovery data for various initial solution concentrations. For the concentration range from 1340 ppb down to 60 ppb, perchlorate recoveries were 87% or higher. Based on this trend, we expected recovered concentration of about 15 mg/l for 30 ppb initial perchlorate concentration and about 3 mg/l for 6 ppb. However, the percent recovery declined at these lower levels, which ultimately affected the effectiveness of the SPE method. We do not yet have an explanation for this decline in recovery. Method Detection Limits Table 4 summarizes the calculation of the method detection limit (MDL) for the SPE analysis method, using the IC configuration in Table 1 and the method specified above. 60 ug/l was chosen as the concentration to perform the MDL analysis. The analysis yielded an MDL of about 37 ug/l. This is about 15 times better than the level calculated for direct injection (Table 5). However, this level is about a factor of 10 higher than the CADHS action level of 4 ug/l. Extracted (mg/l) Calc. Soln Conc. (mg/l) Calc. Soln Conc. (µg/l) Recovery (%) Replicate % Replicate % Replicate % Replicate % Replicate % Replicate % Replicate % Average % Std. Dev Rel. Dev. 20% 20% 20% Deviation from Initial Solution (60 µg/l) 0.81 µg/l or 1.35% MDL Table 3. Perchlorate Recovery Versus Initial Initial (ppb) Recovered (mg/l) Percent Recovery 1* 0 0% 6 0 0% % 12* 0 0% % 30* % 60* % % 450** 78 87% 1340** % * = average of 7 replicates ** = 200 ml filtered instead of 1-L Table 5. Comparison of MDLs for SPE Versus Direct Injection for IC Configuration in Table 1. Configuration Approx. IDL Conc. for MDL analysis Ave. Std. Dev. MDL Direct Injection 500 2,750 2,534 2,657 2,722 2,830 3,075 2,864 2,964 2, SPE 25 to IDL = Instrument Detection Limit. Defined as lowest concentration in which 90% of analyzed samples will have a detectable signal (Standard Methods1992) 4
5 Conclusions Overall, the method improved the MDL for our current configuration by a factor of about 15. However, the overall level is still above regulatory levels. We believe that we can lower the MDL, perhaps to about 10 ug/l, by refining our methodology. Studies by Tsui et al (2000) suggest that peaks can be made sharper and more distinct by increasing the NaOH (they actually used KOH as their solvent) concentration of the IC solvent. However, to reach regulatory levels, it may be ultimately necessary to increase the injection loop. Competing anions may be the greatest problem with the method. We plan on conducting studies using solutions prepared with ground-, surface-, and sea-water to quantify changes in the method s effectiveness with these solutions. Eventually, the method may be coupled with large column injection to achieve extremely low levels of detection and quantification. References Batista, J.R.; McGarvey, F.X.; Viera, A.R Removal of Perchlorate from Waters using Ion Exchange Resins. In: Urbansky, E.T. ed Perchlorate in the Environment. Kluwar Academic/Plenum Publishers. New York, NY. Chapter 13: p Batista, J.R.; Gingas, T.; Viera, A.R Combining Ion-Exchange (IX) Technology and Biological Reduction for Perchlorate Removal. Remediation. 13(1): California Department of Health Services (CADHS) Determination of Perchlorate by Ion Chromatography. Collette, T.W.; Robage, W.P.; Urbansky, E.T Ion Chromatographic Determination of Perchlorate: Analysis of Fertilizers and Related Materials. EPA/600/R-01/026. Dionex. Undated. Analysis of Low s of Perchlorate in Drinking Water and Ground Water by Ion Chromatography. Application Note 12. Ellington, J.J.; Evans, J.J Determination of Perchlorate at a Parts-Per-Billion Levels in Plants by Ion Chromatography. Journal of Chromatography A. 898: Espenson, J.H The Problem and Perversity of Perchlorate. In Urbansky, E.T. ed. Perchlorate in the Environment. Kluwar Academic/Plenum Publishers. New York, NY. Chapter 1: p Federal Register Appendix B Definition and Procedure for the Determination of the Method Detection Limit. 49(209): Gingas, T.M.; Batista, J.R Biological Reduction of Perchlorate in Ion Exchange Regenerant Solutions Containing High Salinity and Ammonium Levels. Journal of Environmental Monitoring. 4:
6 Greenberg, A.E.; Clesceri, L.S.; Eaton, A.D Standard Methods for the Examination of Water and Wastewater. 18 th Edition. American Public Heath Association. Washingon, D.C. Hautman, D.P.; Munch, D.J.; Eaton, A.D; Haghani, A.W Method Determination of Perchlorate in Drinking Water Using Ion Chromatography. USEPA National Exposure Research Laboratory. Cincinnati, OH. Jackson, P.E.; Gokhale, S.; Rohrer, J.S Recent Development in the Analysis of Perchlorate using Ion Chromatography. In: Urbansky, E.T. ed Perchlorate in the Environment. Kluwar Academic/Plenum Publishers. New York, NY. Chapter 5. p Jenkins, T., and L. Escalon Evaluation of Clean Solid Phases for Extraction of Nitroaromatics and Nitramines from Water. U.S. Army Corp of Engineers. Cold Regions Research & Engineering Laboratory. CRREL Special Report Larson, S.L., Felt, D.R.; Davis, J.L. and Escalon, L Analysis of CL-20 in Environmental Matrices: Water and Soil. Journal of Chromatographic Science. 40: Magnuson, M.L.; Urbansky, E.T.; Kelty, C.A Microscale Extraction of Perchlorate in Drinking Water with Low Level Detection by Electrospray-Mass Spectroscopy. Talanta 52: Manuel, C Evaluation of a Passive Sampling Devise (PSD) to Sample Pesticides in Irrigation Water. M.S. Thesis. Washington State University. Pullman, WA. Tsui, D.T.; Clewell, R.A.; Eldridge, E.; Mattie, D.R. Perchlorate Analysis with the AS16 Column. In: Urbansky, E.T. ed Perchlorate in the Environment. Kluwar Academic/Plenum Publishers. New York, NY. Chapter 7. p Viera, A.R The Removal of Perchlorate from Waters Using Ion-Exchange Resins. M.S. Thesis. Department of Civil & Environmental Engineering. University of Nevada, Las Vegas. 6
Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water
Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water Richard F. Jack, 1 Jinshui Che, 2 Lipika Basumallick, 1 and Jeffrey Rohrer 1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA;
More informationMercury Speciation Determinations in Asian Dietary Supplements
Mercury Speciation Determinations in Asian Dietary Supplements Terri Christison, Deepali Mohindra, Frank Hoefler, and Linda Lopez, Thermo Fisher Scientific, Sunnyvale, California, USA Overview Purpose:
More informationOptimizing Sample. Chromium Analyses in Waters. Jane Timm, James Lovick Jr, Raymond Siery, ato 2011 NEMC, Bellevue, Washington
Optimizing Sample Preservation for Hexavalent Chromium Analyses in Waters Jane Timm, James Lovick Jr, Raymond Siery, and Yongtao Li Underwriters Laboratories ato 2011 NEMC, Bellevue, Washington 2011 Underwriters
More informationRapid determination of phosphate and citrate in carbonated soft drinks using ion chromatography
APPLICATION NOTE 7250 Rapid determination of phosphate and citrate in carbonated soft drinks using ion chromatography Authors Jingli Hu and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords
More informationDetermination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography
Application Note 65 Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography INTRODUCTION Preservatives are commonly added to many food products, such as soda, fruit juice,
More informationIdentification and Quantification at ppb Levels of Common Cations and Amines by IC-MS
Application Note 269 Identification and Quantification at ppb Levels of Common Cations and Amines by IC-MS INTRODUCTION Ion chromatography (IC) has been extensively used as the preferred separation technique
More informationIC-MS Environmental Applications - Water Testing. Application Notebook
IC-MS Environmental Applications - Water Testing Application Notebook Table of Contents Environmental Sample Using IC-MS 3 Environmental Applications: Water Testing Acrylamide in Water 4 Trace Urea in
More informationDetermination of Inorganic Ions and Organic Acids in Non-Alcoholic Carbonated Beverages
Application Note Determination of Inorganic Ions and Organic Acids in Non-Alcoholic Carbonated Beverages INTRODUCTION The determination of inorganic anions and cations and organic acids in non-alcoholic
More informationApplication Note. Author. Abstract. Introduction. Food Safety
Determination of β2-agonists in Pork with SPE eanup and LC-MS/MS Detection Using Agilent BondElut PCX Solid-Phase Extraction Cartridges, Agilent Poroshell 120 column and Liquid Chromatography-Tandem Mass
More informationDetection and Quantification of Inorganic Arsenic in Fruit Juices by Capillary Ion Chromatography with Suppressed Conductivity Detection
Detection and Quantification of Inorganic Arsenic in Fruit Juices by Capillary Ion Chromatography with Suppressed Conductivity Detection Hua Yang, Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA
More informationApplication Note 178. Brian DeBorba and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA. 10 Typically, this requires the use of sample
Improved Determination of Trace Concentrations of Perchlorate in Drinking Water Using Preconcentration with Two-Dimensional Ion Chromatography and Suppressed Conductivity Detection Brian DeBorba and Jeff
More informationDetermination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry
Determination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry Application Note Food Safety Authors Chenhao Zhai Agilent
More informationEnrichment of Phospholipids from Biological Matrices with Zirconium Oxide-Modified Silica Sorbents
Enrichment of Phospholipids from Biological Matrices with Zirconium Oxide-Modified Silica Sorbents Xiaoning Lu, Jennifer E. Claus, and David S. Bell Supelco, Div. of Sigma-Aldrich Bellefonte, PA 16823
More informationApplication Note 178 INTRODUCTION
Application Note 78 Improved Determination of Trace Concentrations of Perchlorate in Drinking Water Using Preconcentration with Two-Dimensional Ion Chromatography and Suppressed Conductivity Detection
More informationUtilization of iodide as a perchlorate-laden resin regenerating solution and isolation of perchlorate from simulated ground water samples
Available online at www.sciencedirect.com Separation and Purification Technology 61 (2008) 469 473 Short communication Utilization of iodide as a perchlorate-laden resin regenerating solution and isolation
More informationSample Concentration and Analysis of Human Hormones in Drinking Water
Sample Concentration and Analysis of Human Hormones in Drinking Water Carl Fisher Applications Chemist Ion Chromatography/Sample Preparation Thermo Fisher Scientific March 1, 215 1 The world leader in
More informationDetermination of Amantadine Residues in Chicken by LCMS-8040
Liquid Chromatography Mass Spectrometry Determination of Amantadine Residues in Chicken by LCMS-8040 A method for the determination of amantadine in chicken was established using Shimadzu Triple Quadrupole
More informationSeparation of Chromium (III) and Chromium (VI) by Ion Chromatography
Application Update 65 Separation of Chromium (III) and Chromium (VI) by Ion Chromatography Introduction Chromium in the environment exists primarily in two oxidation states: Cr(III) and Cr(VI). While the
More informationApplication Note. Abstract. Authors. Pharmaceutical
Analysis of xycodone and Its Metabolites-oroxycodone, xymorphone, and oroxymorphone in Plasma by LC/MS with an Agilent ZRBAX StableBond SB-C18 LC Column Application ote Pharmaceutical Authors Linda L.
More informationDetermination of Pharmaceutical Residues in Bovine Milk via LC MS/MS Following Solid Phase Extraction
Determination of Pharmaceutical Residues in Bovine Milk via LC MS/MS Following Solid Phase Extraction Application Note FB0113 Keywords SPE (Solid Phase Extraction), Bovine Milk, JECFA (Joint FAO/WHO Expert
More informationExtraction of Multiple Mycotoxins From Nuts Using ISOLUTE Myco prior to LC-MS/MS Analysis
Application Note AN784 Extraction of Multiple Mycotoxins from Nuts Using ISOLUTE Myco Page 1 Extraction of Multiple Mycotoxins From Nuts Using ISOLUTE Myco prior to LC-MS/MS Analysis This application note
More informationHPLC Analysis of Sugars
HPLC Analysis of Sugars Pre-Lab Exercise: 1) Read about HPLC, sugars and the experiment and its background. 2) Prepare a flowchart as appropriate for the lab exercise. 3) Note the various sugar concentrations
More informationExtraction of Multiple Mycotoxins From Grain Using ISOLUTE Myco prior to LC-MS/MS Analysis
Application Note AN782 Extraction of Multiple Mycotoxins from Grain Using ISOLUTE Myco Page 1 Extraction of Multiple Mycotoxins From Grain Using ISOLUTE Myco prior to LC-MS/MS Analysis This application
More informationINTRODUCTION. Application Note 176
Application Note 76 Determining Sub-ppb Perchlorate in Drinking Water Using Preconcentration/Matrix Elimination Ion Chromatography with Suppressed Conductivity Detection by U.S. EPA Method 34. INTRODUCTION
More informationTOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays.
TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS Objective To make a serial dilution for use in dose/response bioassays. Background The idea behind a bioassay is that the test organism will respond in
More informationMETHOD 8316 ACRYLAMIDE, ACRYLONITRILE AND ACROLEIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
METHOD 8316 ACRYLAMIDE, ACRYLONITRILE AND ACROLEIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 1.0 SCOPE AND APPLICATION 1.1 The following compounds can be determined by this method: Compound Name
More informationThe Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection
Application Note 9 The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection INTRODUCTION The accurate measurement of the amount of sugar in final molasses
More informationDevelopment and Validation of an UPLC-MS/MS Method for Quantification of Mycotoxins in Tobacco and Smokeless Tobacco Products
Development and Validation of an UPLC-MS/MS Method for Quantification of Mycotoxins in Tobacco and Smokeless Tobacco Products Johan Lindholm, Anna Wiernik, Birgitta Grandin, Margareta Curvall Swedish Match
More informationAnalysis of Chloride, Phosphate, Malate, Sulfite, Tartrate, Sulfate, and Oxalate in Red and White Wine
Application Work AW US6-0249-062017 Analysis of Chloride, Phosphate, Malate, Sulfite, Tartrate, Sulfate, Branch Food, stimulants, beverages, flavors Keywords IC; 940; Conductivity; Chloride; Phosphate;
More informationIodine HPLC Assay. Catalog Number: IOD34-H Tests For Research Use Only. Not for use in diagnostic procedures.
Package Insert Iodine HPLC Assay 100 Tests For Research Use Only. Not for use in diagnostic procedures. v. 1.0 Eagle Biosciences, Inc. 20A Northwest Blvd., Suite 112, Nashua, NH 03063 Phone: 866-419-2019
More informationDr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1
Development of an SPE-LC/MS/MS Assay for the Simultaneous Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid in Support of Alzheimer s Research Dr. Erin E. Chambers Waters Corporation Presented
More informationHUMAN LIVER SLICE EXPERIMENT 1 Effects of Propylene Glycol on Ethylene Glycol Metabolism
HUMAN LIVER SLICE EXPERIMENT 1 Effects of Propylene Glycol on Ethylene Glycol Metabolism Determination of Ethylene Glycol, Propylene Glycol, Oxalic Acid and Glycolic Acid BioReliance Study Number Testing
More informationDevelopment of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid
Development of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid Joanne ( 乔安妮 ) Mather Senior Scientist Waters Corporation Data courtesy of Erin Chambers and Mary
More informationSimple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID
Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID James Little, Eastman Chemical Company, Kingsport, TN Overview Phospholipids and
More informationProtein Precipitation for Biological Fluid Samples Using Agilent Captiva EMR Lipid 96-Well Plates
Application Note Clinical Research Protein Precipitation for Biological Fluid Samples Using Agilent Captiva EMR Lipid 96-Well Plates Authors Limian Zhao and Megan Juck Agilent Technologies, Inc. Abstract
More informationFig. 1: Chemical structure of arachidonic acid COOH CH 3
Elimination of Matrix Effects Using Mixed-mode SPE Plate for High Throughput Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS Wan Wang, Suzi Qin, Linsen Li, Warren Chen, Jerry Wang 179, Southern
More informationTHE POWER OF MASS SPECTROMETRY FOR IC ANALYTICAL CHEMISTS. Unleash New Possibilities with Enhanced Detection for IC Applications
THE PWER F MA PECTRMETRY FR IC ANALYTICAL CHEMIT Unleash New Possibilities with Enhanced Detection for IC Applications THE PWER F MA PECTRMETRY Ion chromatography (IC) excels in analyzing ionic species
More informationCHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS
CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
More informationImproved Isolation and Analysis of Mycotoxins from Cereals, Beer and Wine
Improved Isolation and Analysis of Mycotoxins from Cereals, Beer and Wine Application Note Authors Elisabeth Korte, Maha Rudrabhatla, Max Erwine, Jason S Wood Agilent Technologies, Inc. Introduction Trichothecenes
More informationDetermination of perchlorate in environmental waters using a compact ion chromatography system coupled with a single quadrupole mass spectrometer
APPLICATION UPDATE 72507 Determination of perchlorate in environmental waters using a compact ion chromatography system coupled with a single quadrupole mass spectrometer Authors Beibei Huang and Jeffrey
More informationExtraction of Aflatoxin M1 From Infant Formula Using ISOLUTE Myco SPE Columns prior to LC-MS/MS Analysis
Application Note AN807 Extraction of Aflatoxin M From Infant Formula Using ISLUTE Myco Page Extraction of Aflatoxin M From Infant Formula Using ISLUTE Myco SPE Columns prior to LC-MS/MS Analysis This application
More informationRapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition
Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Evelyn Goh Waters Pacific, Singapore APPLICATION BENEFITS This method allows for the simultaneous analysis of 12 water-soluble
More informationInnovations in Large Volume Injection
Innovations in Large Volume Injection Applications of a Chromatographic Zone as Inlet System for GC/MS Jeffery S. Hollis APEX Technologies Silicon Valley, Introduction In typical GC/MS analyses, only a
More informationAnalysis of HMF by HPLC
COST Action 927 Training School Building Skills on the Analysis of Thermal Process Contaminants in Foods 22-26 October 2007, Ankara Analysis of HMF by HPLC Vural Gökmen O O OH Background O COOH O R 2 Carbonyl
More informationGROUNDWATER INFORMATION SHEET. Perchlorate
GROUNDWATER INFORMATION SHEET Perchlorate The purpose of this groundwater information sheet is to provide general information regarding a specific constituent of concern (COC). The following information,
More information[ APPLICATION NOTE ] Oasis PRiME HLB Cartridge for Cleanup of Infant Formula Extracts Prior to UPLC-MS/MS Multiresidue Veterinary Drugs Analysis
Prior to UPLC-MS/MS Multiresidue Veterinary Drugs Analysis Michael S Young and Kim Van Tran Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Efficient, timesaving multiclass/ multiresidue methodology
More informationLC-MS/MS Method for the Determination of Tenofovir from Plasma
LC-MS/MS Method for the Determination of Tenofovir from Plasma Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 687 Key Words SPE, SOLA CX, Hypersil GOLD, tenofovir Abstract
More informationDetermination of Carbachol In Ophthalmic Solutions Using a Reagent-Free Ion Chromatography System
Application Note 9 Determination of Carbachol In Ophthalmic s Using a Reagent-Free Ion Chromatography System INTRODUCTION Carbachol is a choline ester and a positively charged quaternary ammonium compound
More informationCarl Fisher, Terri Christison, Hua Yang, Monika Verma, and Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA
Fast Determination of Lactose and Lactulose in Dairy Products Using a 4 μm Particle Column and High-Performance Anion-Exchange Chromatography with Carl Fisher, Terri Christison, Hua Yang, Monika Verma,
More informationSimultaneous Quantitative Analysis of Total Catecholamines and Metanephrines in Urine Using 500 MG CLEAN UP CCX2 and LC-MS/MS
Simultaneous Quantitative Analysis of Total Catecholamines and Metanephrines in Urine Using 500 MG CLEAN UP CCX2 and LC-MS/MS UCT Part Numbers: CUCCX256 - Clean-Up CCX2 (C8 + Carboxylic Acid) 500mg/6mL
More informationDetermination of Ractopamine and Clenbuterol in Beef Samples
Determination of Ractopamine and in Beef Samples Application Note FB0115 Vincent Bédard 1,François Béland 1,Geneviève Gingras 1, Laine Stewart 2, and Luke Roenneburg 2 1 SiliCycle Inc, Quebec City, Quebec,
More informationHigh Throughput Extraction of Opiates from Urine and Analysis by GC/MS or LC/MS/MS)
High Throughput Extraction of Opiates from Urine and Analysis by GC/MS or LC/MS/MS) Michael Rummel, Matthew Trass, Michael Campognone, and Sky Countryman Phenomenex, Inc., 411 Madrid Avenue, Torrance,
More informationTENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010)
June 2010 TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical
More informationDetermination of Patulin in Apple Juice Using SPE and UHPLC-MS/MS Analysis
Determination of Patulin in Apple Juice Using SPE and UHPLC-MS/MS Analysis UCT Part Numbers ECHLD126-P ENVIRO-CLEAN HLDVB mg/6ml SPE cartridge PE Frit SLDAID21-18UM Selectra DA UHPLC column ( 2.1 mm, 1.8
More informationAuthors. Abstract. Introduction. Environmental
Determination of Ultratrace Amitrol in Water Samples by in situ Derivatization-Solid Phase Extraction-Liquid Chromatography-Mass Selective Detector Application Environmental Authors Gerd Vanhoenacker,
More informationZinc isotopes in the Seine River water, France: a probe of. anthropogenic contamination
Zinc isotopes in the Seine River water, France: a probe of anthropogenic contamination Jiubin Chen*, Jérôme Gaillardet and Pascale Louvat Equipe Géochimie et Cosmochimie, Institut de Physique du Globe
More informationApplication Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products
High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection as a powerful tool to evaluate lactose content in lactose-free labeled products Application Note Authors: C.
More informationLC-MS/MS analysis of Chlorates in Milk and Whey Powder using the Agilent 6470 QQQ
LC-MS/MS analysis of Chlorates in Milk and Whey Powder using the Agilent 6470 QQQ Anthony Sullivan, LC/MS Product Specialist Melanie Mülek and Christoph Müller LC-MS Applications Specialists Hewlett-Packard-Str.
More informationDevelopment of a High Sensitivity SPE-LC-MS/MS Assay for the Quantification of Glucagon in Human Plasma Using the ionkey/ms System
Development of a High Sensitivity SPE-LC-MS/MS Assay for the Quantification of Glucagon in Human Plasma Using the ionkey/ms System Mary E. Lame, Erin E. Chambers, Sukhdev S. Bangar, and Kenneth J. Fountain
More informationDetermination of Ethyl Sulfate Impurity in Indinavir Sulfate Drug Using Ion Chromatography
Application Note 8 Determination of Ethyl Sulfate Impurity in INTRODUCTION Indinavir sulfate is a specific and potent inhibitor of HIV- protease and is widely used in the treatment of AIDS. Indinavir sulfate
More informationPerchlorate: an emerging contaminant
Perchlorate: an emerging contaminant WaterTech 2008 April 18, 2008 Presented by: Erik J. Martin, Ph.D. Outline of Presentation 1. Perchlorate Background 2. Health Effects 3. Exposure Assessment 4. Drinking
More informationAuthor. Introduction. Abstract
Simultaneous Measurement of Sirolimus and Tacrolimus Concentrations in Blood by Semi-Automated Extraction and Liquid Chromatography-Electrospray Ionization Mass Spectrometry Application Author Gary L.
More informationASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC
ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry
More informationAbstract. Introduction
A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine By Shun-Hsin Liang and Sue
More informationDetermination of taurine in energy drinks by high-performance liquid chromatography
Determination of taurine in energy drinks by high-performance liquid chromatography Brad McConn Department of Chemistry, Concordia College, 901 8 th St S, Moorhead, MN 56562 Abstract The concentration
More informationTentu Nageswara Rao et al. / Int. Res J Pharm. App Sci., 2012; 2(4): 35-40
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(4):35-40 Research Article Estimation of Fesoterodine fumarate
More informationDetermination of Carbohydrates in Kombucha Using HPAE-PAD
Determination of Carbohydrates in Kombucha Using HPAE-PAD Beibei Huang, Jingli Hu, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA USA Application Note 1152 Key Words Glucose, Fructose, Sucrose,
More informationMatrix Interferences in ICP-MS: Causes, Effects, and Strategies to Reduce or Eliminate Them
Matrix Interferences in ICP-MS: Causes, Effects, and Strategies to Reduce or Eliminate Them Ruth E. Wolf, Ph. D. and Daniel H. Jones HUMAN HEALTH ENVIRONMENTAL HEALTH 2017 2014 PerkinElmer Matrix Effects
More informationAnalysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection
APPLICATION NOTE Liquid Chromatography Author: Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection Introduction Cannabis sativa, from which
More informationUltivo Applications Terri Sosienski LC/MS Applications Scientist. June 14, 2017
Ultivo Applications Terri Sosienski LC/MS Applications Scientist 1 Ultivo Triple Quad LC/MS Designed for the user! Easy maintenance Compact size Robust, reliable and accurate An ideal tool for the food
More informationRapid and Robust Detection of THC and Its Metabolites in Blood
Rapid and Robust Detection of THC and Its Metabolites in Blood Application Note Forensics/Doping Control Author Stephan Baumann Agilent Technologies, Inc. Santa Clara CA 95051 USA Abstract A robust method
More informationAnalytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products)
Analytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products) The target compound to be determined is 2, 4, 5-T. 1. Instrument Liquid Chromatograph-tandem mass spectrometer (LC-MS/MS)
More information4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column
Clinical, Forensic & Toxicology Applications 4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column By Amanda Rigdon Abstract A rapid, sensitive method
More informationHPLC Analysis with Fluorescence Detection of Chlorophyll Degradation Products Pheophytins and Pyropheophytin in Virgin Olive Oil
HPLC Analysis with Fluorescence Detection of Chlorophyll Degradation Products Pheophytins and Pyropheophytin in Virgin Olive Oil Application Note Authors Xueqi Li, Selina Wang UC Davis Olive Center Robert
More informationDetermination of available nutrients in soil using the Agilent 4200 MP-AES
Determination of available nutrients in soil using the Agilent 4200 MP-AES Application note Agriculture Author Dharmendra Vummiti Agilent Technologies, India Introduction Multielement testing of soil samples
More informationCHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG
CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE IN TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG ANALYTICAL METHOD VALIDATION REPORT FOR ASSAY 43 2.1 Introduction Analytical
More informationRemoval Of Perchlorate From Drinking Water And Ion-Exchange Regenerant Brines
Proceedings of the Annual International Conference on Soils, Sediments, Water and Energy Volume 12 Article 16 January 2010 Removal Of Perchlorate From Drinking Water And Ion-Exchange Regenerant Brines
More informationUPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes
UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F
More informationRapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column
APPLICATION NOTE 21673 Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column Authors Aaron Lamb and Brian King, Thermo Fisher Scientific, Runcorn, UK
More informationNutrient level (EC) in a pot is like a bank
Dirt, Fert and Squirt (1) Supplying Essential Nutrients What are the most common nutritional problems? Too much fertilizer Not enough fertilizer Paul Fisher pfisher@ufl.edu 1 ph too high ph too low 2 Nutrient
More informationAugust Determination of Pindone in Agricultural Products by LC-MS/MS
August 2011 237 LC-MS/MS 23 2 22 Determination of Pindone in Agricultural Products by LC-MS/MS Shizuka S6>ID, Satoru N:BDID and Rieko M6IHJ96 National Institute of Health Sciences: 1 18 1 Kamiyoga, Setagaya-ku,
More informationApplication of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices
Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring
More informationRapid Analysis of Bisphenols A, B, and E in Baby Food and Infant Formula Using ACQUITY UPLC with the Xevo TQD
Rapid Analysis of Bisphenols A, B, and E in Baby Food and Infant Formula Using ACQUITY UPLC with the Xevo TQD Claude R. Mallet Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S This
More informationSugar analysis according to the EU Regulation using amperometric detection and Metrosep Carb 2-150/4.0
Sugar analysis according to the EU Regulation using amperometric detection and Metrosep Carb 2-150/4.0 Branch Food and beverages Bottle head 6.1602.200 Adsorber tube for Dosing Unit 6.1619.000 Keywords
More informationStrengthening Results. Advanced Chromatography with Mass Detection
Strengthening Results Advanced Chromatography with Mass Detection UV Detection Why Not Use UV For Everything? Identification Difficult selectivity in complex matrices Sensitivity Need sufficient molar
More informationFACTORS AFFECTING WATER QUALITY
TECHNICAL PAPER WATER QUALITY PLANT HEALTH FACTORS Water quality is one of the most important factors affecting plant growth, as unwanted components in water can interfere with nutrient availability and
More informationFig.1. Denatonium benzoate (DB) chemical structure
ILIADE code 280 CLEN Method Determination of Denatonium Benzoate in Alcoholic Products by HPLC-UV 1 Scope The purpose of this method is verification of fulfilment of the legislative requirements on denatured
More informationAnalysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC
Analysis of Counterfeit Antidiabetic Drugs by UPLC with the Agilent 1 Infinity Mobile LC Application Note Small Molecule Pharmaceuticals & Generics Author Sonja Schneider Agilent Technologies, Inc. Waldbronn,
More informationLC/MS/MS of Trichothecenes and Zearalenone in Wheat Using Different Sample Prep Methods
LC/MS/MS of Trichothecenes and Zearalenone in Wheat Using Different Sample Prep Methods Application Note Food Testing & Agriculture Authors Nick Byrd and Danielle Sweeney Campden BRI Gloucestershire UK
More informationDetermination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.
Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are
More information[ APPLICATION NOTE ] The Separation of 8 -THC, 9 -THC, and Their Enantiomers by UPC 2 Using Trefoil Chiral Columns INTRODUCTION APPLICATION BENEFITS
The Separation of 8 -THC, 9 -THC, and Their Enantiomers by UPC 2 Using Trefoil Chiral Columns Jacquelyn Runco, Andrew Aubin, and Catharine Layton Waters Corporation, Milford, MA, USA APPLICATION BENEFITS
More informationRITONAVIRI COMPRESSI RITONAVIR TABLETS. Final text for addition to The International Pharmacopoeia (July 2012)
July 2012 RITONAVIRI COMPRESSI RITONAVIR TABLETS Final text for addition to The International Pharmacopoeia (July 2012) This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications
More informationRapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free Ion Chromatography System
Application Note 69 Rapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free Ion Chromatography System INTRODUCTION Soft drinks are complex mixtures containing a variety
More informationThe Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements
The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements Application Note 215 Joan Stevens, PhD, Kirby Reed (Gilson,
More informationElemental Scientific. Automated Speciation. prepfast-ic Chromium Speciation for Toy Materials (EN 71-3) ICP ICPMS AA
prepfast-ic Automated Speciation prepfast-ic Chromium Speciation for Toy Materials (EN 71-3) Presented at the 015 European Winter Plasma Conference Authors: Aurelien Viscardi, Kyle Uhlmeyer & Patrick Sullivan
More informationHyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.
International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik
More informationDetermination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography
Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Application ote Food Safety Authors Chen-Hao Zhai and Yun Zou Agilent Technologies Co. Ltd.
More informationRapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases
Clinical, Forensic & Toxicology Applications Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases By Shun-Hsin
More informationValidation of Changes to the USP Assay Method for Ibuprofen Tablets
Validation of Changes to the USP Assay Method for Ibuprofen Extraction and Filtration Techniques Lynn Massad, Pam Anderson, James Ward, Philip Burns, and Ranga Velagaleti* This article discusses changes
More informationIn-stream attenuation of neuro-active pharmaceuticals and their
In-stream attenuation of neuro-active pharmaceuticals and their metabolites Supporting Information Analytical Methods and Figures (10 pages) Jeffrey H. Writer, Ronald C. Antweiler, Imma Ferrer, Joseph
More information