Strengthening Results. Advanced Chromatography with Mass Detection

Transcription

1 Strengthening Results Advanced Chromatography with Mass Detection

2 UV Detection Why Not Use UV For Everything? Identification Difficult selectivity in complex matrices Sensitivity Need sufficient molar absorptivity Method Development Peak assignments require confirmation runs Quantitation Relatively high detection limits and narrow dynamic ranges Definitive Confirmation May require confirmation analysis Structural Information Limited information 2

3 What Are Some of the Benefits to Mass Detection? UltiMate LC System MSQ Plus Mass Detector 3

4 Benefits: Reduce Matrix Background Interference Mass detectors allow analyte-specific detection within matrices Column Hypersil Gold PFP 50 x 2.1, 1.9 um Gradient H2O:ACN 0.1% FA Flow Rate 500 ul/min Injection 2 ul Analyte 10 ppm Detector nm 4

5 Benefits: Improve Limit of Quantitation Mass detectors allow analyte-specific detection within matrices Column Hypersil Gold PFP 50 x 2.1, 1.9 um Gradient H2O:ACN 0.1% FA Flow Rate 500 ul/min Injection 2 ul Analyte 10 ppm Detector ESI Positive Capillary 4.5 kv Probe 550 C Standard in Urine Water 152 m/z 152 m/z m/z m/z Filter contaminants, isolate analyte, and increase sensitivity Minimize sample preparation, simplify workflow, increase throughput 195 m/z 195 m/z 5

6 Benefit: Improve Limit of Quantitation Mass detectors allow sensitive, robust, multi-component quantitation across large linear dynamic ranges Column Hypersil Gold PFP 100 x 2.1, 1.9 um Gradient (Ternary) H2O:MeOH:ACN 0.1% Acetic Acid Flow Rate 1,000 ul/min Injection 2 ul Analyte Variable Detector ESI Positive Capillary 4.5 kv Probe 450 C 6

7 Benefit: Improve Limit of Quantitation Mass detectors allow sensitive, robust, multi-component quantitation across large linear dynamic ranges Column Hypersil Gold PFP 100 x 2.1, 1.9 um Gradient (Ternary) H2O:MeOH:ACN 0.1% Acetic Acid Flow Rate 1,000 ul/min Injection 2 ul Analyte Variable Detector ESI Positive Capillary 4.5 kv Probe 450 C Improve limits of detection and quantitation for multi-component analyses 7

8 Benefit: No Chromaphore required Mass detectors ionize and detect analytes that do not exhibit strong UV absorbencies Column Hypersil Gold PFP 100 x 2.1, 1.9 um Gradient H2O:MeOH Ammonium Formate Flow Rate 500 ul/min Injection 2 ul Analyte Triacetone triperoxide (TATP) Detector APCI Positive Corona 30 ua Probe 350 C TATP Common for terrorist activities 348 m/z No significant absorption No florescence activity Highly unstable TATP (222 m/z) + Fragment (108 m/z) + Ammonium (18 m/z) Heat, friction, shock, and UV light TATP Overcome limitations associated with conventional LC detection 8

9 Benefit: Detect Inorganic Analytes Mass detectors allow inorganic analysis concurrent with organic analysis Column Hypercarb 100 x 2.1, 5 um Gradient H2O:MeOH 1% FA Flow Rate 300 ul/min Injection 5 ul Analyte Gunpowder Extraction ~ 50 ug/ml Detector ESI Positive/Negative Capillary 3.5 kv Probe 550 C Cations Anions Time (min) Increase throughput by using an all-in-one detector 9

10 Benefit: Confirm Identification Mass detectors allows confident and efficient identification of analyte peaks Method 1 Column Hypersil Gold PFP 100 x 2.1, 1.9 um Gradient Caffeine Cocaine H2O:ACN 0.1% Acetic Acid Flow Rate Method 2 1,000 ul/min Injection Caffeine Cocaine 2 ul Analyte 500 ppb Detector ESI Positive Capillary 3.5 kv Probe 450 C 195 m/z Caffeine Cocaine 304 m/z Quickly identify peaks after changing columns, gradients, or flow rates 10

11 Benefit: Additional Information on Impurities Mass detectors strengthen purity analysis by generating impurity spectra Column Hypersil Gold 100 x 2.1, 1.9 um Isocratic H2O:MeOH AU Flow Rate 800 ul/min Injection 5 ul Analyte Simvastatin Detector (UV) 254 nm Detector (Mass) ESI Positive/Negative Capillary 3.0 kv Probe 550 C Degradant m/z 4 Statin Minutes Statin Degradation Lactone Hydroxy Acid 8 9 Identify the mass, structure, and source of impurities 11

12 Benefit: Definitive Confirmation Mass detectors allow systematic and reproducible fragmentation of analyte for multiple orders of confirmation Column Hypersil Gold 100 x 2.1, 1.9 um Isocratic H2O:MeOH Flow Rate 800 ul/min Injection 5 ul Analyte Illicit Drugs Detector ESI Positive Capillary 3.5 kv Probe 550 C Relative Abundance Psilocin Seized Evidence Increase Cone Voltage Increase Cone Voltage 115 m/z Loss of OH Loss of C 2 H 4 m/z Loss of HN(CH 3 ) 2 m/z 132 m/z 160 m/z 205 m/z Psilocin Time (min) m/z Effectuate compound-specific fragmentation and match to theoretical behavior 12

13 Benefit: Library Search Mass detectors allow matching of spectra against user-generated libraries Column Hypersil Gold PFP 100 x 2.1, 1.9 um Gradient H2O:MeOH Ammonium Formate Flow Rate 500 ul/min Injection 2 ul Analyte Soil Extraction LOD 0.2 ug/kg Detector APCI Negative Corona 30 ua Probe 350 C RT 7.18 min Trinitrotoluene 98.19% Probability Unknown Soil Sample Library Data Confidently screen samples for target analytes using library matching 13

14 Mass Detection Strengthen Chromatography Easier Identification Ionize analyte with low molar absorptivity Create positive and negative ions simultaneously Greater Sensitivity Enhance selectivity Filter contaminants Faster Method Development Simplify peak assignment Enhanced Quantitation Isolate analytes More Definitive Confirmation Match against spectral libraries Induce compound-specific fragmentation Structural Information Identify impurities Increase sensitivity Improve quantitation UltiMate LC System MSQ Plus Mass Detector 14

15 How Does Mass Detection Work? UltiMate LC System MSQ Plus Mass Detector 15

16 What is a Mass Detection? Mass detection is the measurement of the mass-to-charge ratio of individual molecules that have been converted to gas phase ions. Mass Spectrum: A plot of mass to charge (m/z) vs. relative or absolute intensity. Light Filter Mass Filter 16

17 MSQ Plus Mass Detector Single Quadrupole Strengthen chromatography with enhanced mass detection Mass confirmation Robust quantitation Structural elucidation Positive identification FastLoc Probe Entrance Cone Exit Cone Ion Bright Detector Cone Wash Transfer Lens Quadrupole Analyzer Analyte from LC Column Vacuum Signal to Data System 17

18 MSQ Plus Mass Detector: Advanced LC Detection Expanded Application Versatility Extended Da mass range FastLoc TM ESI and APCI Probes Maximum flow rate 2 ml/min for both ESI and APCI Minimized Operation Costs Patented titanium entrance cone Increased Sensitivity Scan speeds up to 12,000 amu/sec Patented Cone Wash technology 18

19 MSQ Plus Mass Detector: Advanced LC Detection Maximized Sample Throughput Patented M-path Source Wipe clean surfaces for easy maintenance Greater User Confidence Automatic tune and calibration Improved Quantitative Ability Source CID (Collision Induced Dissociation) for ion fragmentation Simultaneous quantitative and qualitative determinations by combining Full Scan and SIM. Multiple scan modes with one injection (+/-, FS, SIM, overlapping segments) 19

20 Example Applications UltiMate LC System MSQ Plus Mass Detector 20

21 A Single Ternary Method for 14 Drugs Fourteen drugs baseline separated in twelve minutes by employing UHPLC/MS with a ternary solvent gradient. Identified Quantified Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; ternary gradient of water, acetonitrile, and methanol with acetic acid at 1 ml/min; 1 ul injection; ESI Positive 21

22 22 A Single Ternary Method for 14 Drugs

23 Hallucinogenic Mushrooms in Chocolate Easily separate psilocybin and psilocin using UHPLC/MS, avoiding detection difficulties such as low concentration, thermal lability, and matrix interference that are typical with other techniques Psilocybin Psilocin Standard Psilocybin (RT: 0.65min) Relative Abundance Psilocybin Psilocin Chocolate Relative Abundance Psilocin (RT: 2.26min) Time (min) m/z Identified Confirmed Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; ternary gradient of water, acetonitrile, and methanol with acetic acid at 1 ml/min; 2 ul injection; ESI Positive 23

24 Confirmation of Psilocin Definitively confirm the presence of illicit drugs using UHPLC/MS, by creating and searching spectral libraries for fragmentation patterns produced through in-source collision induced dissociation Relative Abundance Psilocin (low cone voltage) m/z Relative Abundance In-Source CID (medium cone voltage) Relative Abundance In-Source CID (high cone voltage) m/z Confirmed Rodriguez Cruz, Sandra E., Analysis and Characterization of Psilocybin and Psilocin Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS) with Collision-Induced-Dissociation (CID) and Source-Induced-Dissociation (SID), Microgram Journal, Volume 3 Numbers 3-4 (2005) m/z Confirmed 24

25 LSD in Candy Hearts Positively identify LSD within a complex matrix using UHPLC/MS, requiring no downtime for instrument bake-out and clean-up Relative Abundance Standard Candy Heart LSD LSD Relative Abundance LSD (RT: 4.49min) Time (min) m/z Identified Confirmed Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; ternary gradient of water, acetonitrile, and methanol with acetic acid at 1 ml/min; 2 ul injection, ESI Positive 25

26 Marijuana in Brownies Efficiently separate and detect THC, cannabinol, and cannabidiol using UHPLC/MS, consuming only milligram amounts of evidence Standard Cannabidiol THC Cannabinol Cannabidiol (RT: 4.1min) Relative Abundance Brownie THC Relative Abundance THC (RT: 5.1min) Cannabinol (RT: 5.4min) Time (min) m/z Identified Confirmed Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; ternary gradient of water, acetonitrile, and methanol with acetic acid at 1 ml/min; 2 ul injection; ESI Positive 26

27 Pseudoephedrine in Tablets Quantify ephedrine, pseudoephedrine, methamphetamine, amphetamine, and 3,4-MDMA using UHPLC/MS; and, elucidate the current manufacturing pathways of clandestine chemistry Relative Abundance Standard Tablet Ephedrine Pseudoephedrine Pseudoephedrine Amphetamine Methamphetamine 3,4-MDMA Area (10 4 ) Pseudoephedrine (RT: 2.60min) R 2 = LOQ = 1.2 ppb LOD = 0.4 ppb Time (min) Concentration (ppb) Identified Quantified Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; binary gradient of water and acetonitrile with acetic acid at 1 ml/min; 1 ul injection; ESI Positive 27

28 Pseudoephedrine in Tablets Definitively confirm the presence of illicit drug product using UHPLC/ MS, by creating and searching spectral libraries for fragmentation patterns produced through in-source CID Relative Abundance Relative Abundance Molecular Ion (low cone voltage) In-Source CID (high cone voltage) Pseudoephedrine (RT: 2.60min) m/z Hypersil GOLD PFP 1.9um, 100 x 2.1 mm; binary gradient of water and acetonitrile with acetic acid at 1 ml/min; 1 ul injection; ESI Positive 28

29 Salt Forms of Product Characterize illicit drugs and their precursors by distinguishing unique salt forms with low mass, polarity switching UHPLC/MS Bromide Iodide 20 Relative Abundance Phosphate Chloride Nitrate Chlorate Sulfate Perchlorate Amphetamine Meth 3,4-MDMA Cocaine Area (10 4 ) Chloride (RT: 1.59min) R 2 = LOQ = 50.0 ppb Time (min) Concentration (ppm) Identified Quantified Hypercarb 3um, 50 x 2.1 mm; binary gradient of water and methanol with formic acid at 300 ul/min; 2 ul injection; ESI Positive and Negative 29

30 Gunpowder in Particles Determine the make and manufacturer of gunpowder found in particles, pellets, and residue by characterizing their unique salt compositions through low mass, polarity switching UHPLC/MS Hodgdon Triple Seven Sodium Sodium Hodgdon Pyrodex Normalized Abundance Potassium Nitrate Gluconate Perchlorate Dicyandiamide Potassium Nitrate Gluconate Perchlorate Dicyandiamide Time (min) Benzoate Time (min) Benzoate Hypercarb 5um, 100 x 2.1 mm; binary gradient of water and methanol with formic acid at 300 ul/min; 5 ul injection; ESI Positive and Negative 30

31 Explosive Compounds in Soil Simultaneously analyze nitroamines, nitroaromatics, nitrate esters, and peroxide explosives by UHPLC/MS in under 15 minutes. Confirm the identifications by spectral library search. Peak Compound 1 HMTD Match scores 2 EGDN 3 TNB 4 DEGDN 5 HMX 6 1,3-DNB 7 RDX Target spectrum 8 TNT 9 2,6-DNT 10 NG 11 2,4-DNT 12 4-A-2,6-DNT Library spectrum 13 TATP 14 TETRYL 15 TMETN 16 2-A-4,6-DNT 17 PETN 31 Identified Confirmed

32 MSQ Plus Mass Detector: Advanced LC Detection For cost conscious chemists who require increased productivity through dependable simplicity, the Thermo Scientific MSQ is a turnkey UHPLC/MS solution providing superior chromatographic efficiency and enhanced mass detection with unsurpassed ease of use, performance, and ruggedness. 32

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