Tbp. Per Relative mrna levels Circadian Time. Liver weight/ body weight (%) n.s. Pernull
|
|
|
- Rodger Rice
- 6 years ago
- Views:
Transcription
1 Liver weight/ ody weight (%) Dy Body weight (g) Reltive mrna levels Reltive mrna levels Reltive mrna levels Reltive mrna levels Dy Per1 Per2 Per3 Tp Cirdin Time Cirdin Time Mouse ge (week) Cirdin Time d Cirdin Time 2- Pernull (hour) LD DD (hour) LD DD Supplementry Figure 1. Chrteriztion of the si physiologil nd ntomil profiles of mie. () qrt-pcr nlysis showing the expression of Per1, Per2 nd Per3 mrna in nd liver t indited times. Note ler irdin rhythms of Period genes in re ompletely olished in mie. Vlues represent the men ± SEM. (n = 3). () Representtive doule-plotted togrm of nd mie. Mie (n = 1 t eh genotype) were initilly housed in 12L:12D light-drk yle (LD; light on 8: nd light off t 2:) nd susequently trnsferred to onstnt drkness (DD). mie entrined their tivity rhythms to the environmentl LD yles, nd showed ler irdin rhythmiity (period length: ±.23 h, n = 1) in DD onditions. Note tht mie, whih lk the lok osilltory mhinery, showed no rhythms in DD onditions ut still displyed dy-night differene of tivity in LD yle due to the msking effet of light. White nd gry kground indites lights on nd off, respetively. () The hnge of ody weight of nd mie during postntl development. mie did not show ny prominent normlity in development, nd developmentl weight gin ws virtully identil to tht of wild-type mie, when fed norml diet. Men ± SEM. (n = 7);, sttistilly not signifint. (d) The profile of ody-weight- normlized liver weight of nd mie mintined on stndrd diet smpled t 12 weeks of ge. Men ± SEM.; Student s t-test (n = 1)., sttistilly not signifint. Tp, TATA-ox inding protein.
2 j k h i f g d e l Supplementry Figure 2. Histologil setions in vrious orgns in nd mie. We performed hemtoxylin nd eosin stining in () smll intestine, () liver, () lrge intestine (olon), (d) pnres (exorine glnd nd islet of Lngerhns), (e) kidney (ortex), (f) drenl (ortex, (g) lung (lveolus nd ronhil epithelium), (h) testis, (i) skin (epithelium nd hir follile), (j) spleen (white pulp nd red pulp), (k) rdi musle, nd (l) ererl ortex in nd mie. Prformldehyde-fixed prffin emedded setions ( mm in thikness) were histologilly nlyzed y lightmirosopy (Olympus). Note no polyploidy ws oserved in ll tissue exmined exept liver. Sle rs, 1 mm.
3 (Per1+/+Per2+/+Per3+/+) Per1KOPer2Brdm1 (Per1-/-Per2m/mPer3+/+) 2 (Per1-/-Per2m/mPer3-/-) Nuler size of 6 3 Nuler size of 3 Per3KO (Per1+/+Per2+/+Per3-/-) Per2Brdm1 (Per1+/+Per2m/mPer3+/+) Per1KO (Per1-/-Per2+/+Per3+/+) Per1-/-Per2+/+Per3+/ Per1-/-Per2+/+Per3+/ Per1+/+Per2m/mPer3+/+ Perentge (%) Per1+/+Per2+/+Per3-/ Perentge (%) 3 Per1+/+Per2m/mPer3+/ Per1+/+Per2+/+Per3-/ Per1+/+Per2m/mPer3-+/ Per1+/+Per2m/mPer3-/ 1 1 Nuler size (mm2) Nuler size (mm2) Supplementry Figure 3. Effet of Period genotype on nuler size of heptoytes in vivo. () Representtive exmples of Hoehst-3332 stined nulei round entrl vein () nd portl vein () in livers from nd indited Period mutnt mie. Quntittive dt show the frequeny distriution of nuler size of entro-mid loulr heptoytes () () nd periportl heptoytes () () nlyzed y Hoehst-3332 histologil stining. Note genotype speifi enlrgement ours only in ut not in. Vlues re men ± SEM., n > 1 ells, 3 experiments. Sle rs, 1 mm.
4 Perentge (%) Perentge (%) Perentge (%) Time (min x 1) Time (min x 1) Perentge (%) Time (min x 1) Time (min x 1) Mononuleted heptoytes Bipolr Heptoyte type Bi-nuleted heptoytes Mitoti types Tripolr Tetrpolr Rtio of proliferting ells Trinuleted heptoytes d Time of first ell dividing fter seeding Cell seeding (i) 1st dividing (ii) Intervl etween 1st nd d ell d dividing Dispperne of nuler envelope Intervl etween d nd 3rd ell (iii) 3rd dividing Durtion of ell Cytokinesis & kryokinesis Repperne of nuler envelope Timing of first nd intervls etween ell Durtion of ell Mononulete () () () Mononulete 1st d 3rd Binulete n.d. () () () Binulete n.d. 1st d 3rd e Cytokinesis filure without ontrtile ring formtion : hh:mm : 1: 1:2 1: 2: 3: mm C C f mm ** Norml ytokinesis / mononulete / iulete / mononulete / inulete Cytokinesis filure without ontrtile ring formtion Asission filure Supplementry Figure. Heptoyte primry ulture: si dt nd time-lpse imges of heptoyte primry ulture. (-d) Quntittive nlyses of ell types (), types (), nd the rtio of proliferting ells () from time-lpse imges of heptoytes in ulture. The perentge of mono-, i-, nd tri- nulete heptoytes (), the perentge of ipolr, tripolr nd tetrpolr of heptoytes (), nd the perentge of proliferting ells () show no differene etween nd heptoytes. (d) Detiled nlysis of mitoti events in heptoytes in primry ulture. Shemti presenttion of time ourse of ultured heptoytes (left-upper figure) nd the third mitoti events (left-lower figure). After ell seeding, mitoti events were oserved for 3 times, nd we mesured intervls etween these events. (i) indites the time of first ell fter seeding, (ii) indites the intervl etween first nd seond ell, nd (iii) indites the intervl etween seond nd third ell. We lso mesured the durtion of ell, whih indites the time etween the dispperne nd repperne of the nuler envelope in the first, the seond nd the third mitosis. Aove quntifition ws performed in eh ell type (mono- nd i-nulete heptoytes). Note the intervl time etween eh ell (upper pnel), nd durtion of ell (lower pnel) hve no signifint differene etween nd heptoytes. All dt in (-d) strongly suggest tht there re no genotype-speifi si mitoti events in the present ulture system. Vlues re men ± SEM. (, n = 26;, n = 717; experiments)., sttistilly not signifint. n.d., not deteted. (e), Representtive time-lpse imges of ultured heptoytes showing ytokinesis filure without ontrtile ring formtion. We lssified ytokinesis of heptoytes into three groups: (1) norml ytokinesis, (2) ytokinesis filure without ontrtile ring formtion, nd (3) sission filure (see Supplementry Movie 1). (1) nd (3) re presented in Fig. 3, nd here (2) is shown: Yellow rrows indite the loliztion of nulei nd hromosomes of dividing heptoytes. Crtoons orresponding to time-lpse imges re shown with the numer of hromosomes () in the gmetes (n). (f) Quntifition of the perentge of three types of ytokinesis from time-lpse imging: (1) norml ytokinesis, (2) ytokinesis filure without ontrtile ring formtion, nd (3) sission filure. Among three groups, only the sission filure is inresed in heptoytes ompred to. Vlues represent the men ± SEM., **P<.1, P<.1 y Student s unpired t-test (, n = 26 ells;, n = 717 ells from experiments for eh group. Continued to next pge.
5 g Asission filure followed y norml ytokinesis -1: hh:mm : : 1: 1:2 1: 2: mm mm 3: : 8: 1: 1:1 1: 16:1 16:2 16: 17: 23:3 28:3 38:3 Supplementry Figure. (ontinued) (g) Representtive time-lpse imges of ultured heptoytes showing formtion of enlrged nuleus with n sission filure followed y norml ytokinesis (see Supplementry Movie 2). First, the ell with (sed on Hoehst stining) nuleus entered into S-phse to eome, then progressed to M-phse ut filed sission, resulting in inuler heptoyte ( 2). This ell then strted seond S-phse ( 2), went into seond M-phse with oth nulei fusing, performed ytokinesis suessfully, nd then eme 2 mononuleted heptoytes eh with single enlrged nuleus ( 1; 2 ells). Thus, two ell yles, the first filing sission, led to polyploid heptoytes with single enlrged nuleus. Yellow rrows indite the loliztion of nulei nd hromosomes of dividing heptoytes. Blue rrows exhiit interellulr ridges etween dughter ells. Below, rtoons orresponding to time-lpse imges re shown, demrting opy numer of hromosome. The sttes of interellulr ridge (round 2- min) etween dughter ells re lue-underlined. See text for further detils. Sle rs (e, g), mm.
6 pakt (Ser73) / pnakt (%) pakt(thr38) / pnakt (%) IR IRS1 IRS PI3K PDK Sh1 Sos1 Rs p8 Phospho-Akt (Thr38) Phospho-Akt (Ser73) Akt -Rf pnakt S6 mtor Mek1/2 Phospho-S6 Riosoml Protein (Ser23/236) RhoA S6 Riosoml Protein F-tin Phospho-p/2 MAPK() (Thr22/2 /Thr18/187) Cytokinesis p/2 MAPK() tin (nm,1min) pakt (Thr38) ps6 (Ser23/236) nm nm nm nm nm nm pakt (Ser73) / totl Erk (%) ps6 (Ser23/236) / S6 (%) (Thr22/2, Thr18/187) nm nm nm nm nm nm Supplementry Figure. Kinse tivities in insulin signling pthwys in ultured heptoytes: genotype speifiity. () Shemti representtion showing two mjor insulin downstrem signling pthwys in liver. () Immunolots of ultured heptoytes with insulin tretment s indited. () Quntittive dt displyed the level of phosphoryltion of Akt (Thr38 nd Ser73), S6 (Ser23/236), nd (Thr22/2, Thr18/187). Although insulin dose-dependently tivtes ll these kinses in oth genotypes, the extent of tivtion of ws severely dmped in heptoytes. Duplite experiments showing indited vlue of one smple t nm insulin tretment eing 1 %.
7 GS CT12/ CT/ PCS (GS+) ** Heptoytes surrounding PCS (GS-) 1 CT12/ CT/ Nuler size 2 3 Nuler squre (mm2) / ell d (Brown)/ GS(Cyn) CT/ Cirdin hnge of stinings in mture heptoytes surrounding PCS CT/ Perentge of perk -positive ells (%) CT CT/ e CT/ CT CT8 CT12 CT16 CT2 perk BrdU f BrdU merged g Mouse ge (weeks) Ki67 i Delopmentl hnges of BrdU-lelled ells Mie ge (weeks) BrdU positive ell/ x1 mm2 Development of nuler sizes of heptoytes Nuler size mm2 h Ki67 BrdU positive ell/ x1 mm2 Mie ge (weeks)
8 Supplementry Figure 6. Distriution of in perientrl stem ells (PCS) nd its neighoring mture heptoytes. () Perientrl stem ells (PCS) re mrked y glutmine synthetse (GS) (Wng et l. 21). PCS leled y GS were distriuted round in oth nd mie. Note the nuler size of PCS were two-third diploid nd one-third tetrploid () in (lk rrows, left figure), nd this tendeny does not hnge in heptoytes (lk rrows, right figure). In ontrst, heptoytes surrounding PCS, presumly the desendnts of PCS (Wng et l. 21), showed mostly in (lue rrows, left figure), ut mostly - 16n high polyploidy in heptoytes (red rrows, right figure). Lower grph represents the quntifition of nuler sizes in PCS nd heptoytes surrounding PCS. Note the nuler sizes of PCS do not hnge etween two genotypes, lthough there is mrked enlrgement of mture heptoytes in Pernull strin. () immunohistohemistry ner region t CT nd CT12 in nd mie. Note the PCS ells show fint expression of even t CT, the pek time of expression. () Doule stining of (rown) nd GS (yn), mrker of heptoytes surrounding the entrl vein (), in liver of nd mie. Note tht ws expressed in entro-midloulr heptoytes () entering the, ut not in periportl heptoytes () surrounding the portl vessels () in mie t CT. However, signls in heptoytes were no longer oserved in mie, lthough glutmine synthetse ws not ltered in either genotype. (d) Quntittive immunohistohemil nlysis of irdin expression of positive ells in mture heptoytes surrounding PCS. Perentge of positive ells in mture heptoytes surrounding PCS were ounted in (lk) nd mie (red) t h intervls in onstnt drk onditions. Vlues represent the men ± SEM., P<.1 (n = 3 for eh time point in nd mie)., no sttistil signifine. (e) Dul immunofluoresene with BrdU (green) nd (red) ntiodies in liver. Their merged imge is shown in lower left. Arrows indite tht ell is in S-phse. BrdU (.1 mg/g ody weight; Sigm, St. Louis, MO) ws injeted t 1 weeks of ge 1hr efore srifie t dwn (ZT). Note BrdU leled ell ws on the peripherl order ( side) of expressing ell group in hepti loule. (f) Lotion of BrdU positive ells in liver. For omprison, we lso heked BrdU lelling (DAB; rrow) in heptoytes, ut, sine heptoytes do not show immunoretivity, it is impossile to relily relte the two. In single stined histologil setions, however, we found tht BrdU ells were lso loted on the peripherl order of lrge sized polyploid ells. (g) Ki67-immunopositive ells in the hepti loule (h) of nd dult mie. (h, i) Quntittive nlyses of the development of nuler sizes (h) nd the numer of BrdU-inorported ells (i) in nd liver (men ± SEM; n= t eh developmentl stge for eh genotype; injetion protool nd tissue preprtion re the sme s (e). (i) In oth genotypes, the verge nuler size inreses ording to the development from 2 to 1 weeks. Although there ws no signifint differene etween genotype in 2 weeks old mie (see lso Fig. 1g), the inrese of nuler ploidy eme prominent in weeks-old mie, nd further inresed until 1 weeks. In two-wy ANOVA, the min effet of time nd genotype were oth signifint. (j) Developmentl nlysis demonstrted tht the shrp ge-dependent deline of BrdU lelling in oth genotypes: the tul lelling of heptoytes derese more thn times y 1 weeks, ompred to 2 weeks. In twowy ANOVA, only the min effet of time ws signifint in nd. These dt lerly demonstrted tht ge-dependent inrese in ell size (showing the degree nd the proportion of polyploidy ells) is inversely proportionl to the numer of proliferting ells. Sle r, Brs = 2 μm (e), mm (,, f), 2 mm (, g). See lso the following Supplementry Disussion for Fig. 6.
9 Conentrtion (fmole/ l) Conentrtion (fmole/ l) Conentrtion (fmole/ l) pmek1/2 / totl Mek (%) Conentrtion (fmole/ l).3 Mek1.6 Mek2 (nm, 1min) pmek1/2.2. Mek1/2 tin.1. CT6 CT18 CT6 CT18.2. CT6 CT18 CT6 CT nm nm nm nm nm nm / CT6 / CT18 / CT6 / CT Mkp1 (Dusp1) ** * Mkp2 (Dusp) Mkp3 (Dusp6)...3 Mkp (Dusp9) Mkp (Dusp1) Mkp6 (Dusp1) Mkp7 (DUSP16) P1(DUSP2) Vhr (Dusp3) * Gm337 (Dusp) Pyst2 (Dusp7) Jkp (Dusp22) Tp ** ** * Supplementry Figure 7. Enzymes regulting phosphoryltion in heptoytes. () qpcr nlyses of up-strem kinses Mek1 nd Mek2 mrna t sujetive dy (CT6) nd sujetive night (CT18) in nd livers. Neither Mek1 nor Mek2 showed signifint differene for dy-night nd genotypes. () Immunolots of Mek1/2 kinses in ultured heptoytes fter tretment with insulin. Although Mek1/2 phosphoryltion is sensitive to insulin tretment, there ws no genotype differene t eh onentrtion of insulin. () qpcr nlyses of Erk phosphtses, nmely Mkp fmily, t CT6 nd CT18 in nd livers. Note tht Mkp1 ws the only gene showing dily rhythm in, nd inresed in mie. Vlues re the men ± SEM. (n = ), *P <., **P <.1, P <.1.
10 Supplementl Figure 8. Visuliztion of previously reported ChIP-seq signls for BMAL1 (lue) nd CLOCK (green) on Mkp1 (Dusp1) nd Per2. Here we present the previously reported Mkp1 (Dusp1) nd Per2 dt on ChIP-seq signls for BMAL1 (lue) y Annyev et l. in J Biol Chem 289, 13-2, 21 (SRA ID: SRX1 nd SRX2); nd CLOCK (green) y Yoshitne et l. in Mol Cell Biol 3, , 21 (SRA ID: DRX1196). In oth reports, livers t ZT8 were nlyzed, nd pek lling nlysis reveled signifint peks in the proximity of this E-ox. BigWig files tht orrespond to eh ChIP-seq experiment were downloded from the ChIP-Atls wesite ( nd visulized with the Integrtive Genomis Viewer ( Arrow indites the position of the Mkp1 E-ox, to whih the inding of CLOCK nd BMAL1 hs een onfirmed in ChIP-seq experiments, leit with lower ffinity thn to the Per2 E -ox (sterisk).
11 Perentge (%) Control 2 mm U126 8 mm BCI tin 8 h fter infetion 72 h fter infetion 96 h fter infetion 12 h fter infetion Lenti- GFP Lenti- GFP- Mkp1 Mkp1 1 h fter infetion 168 h fter infetion 192 h fter infetion 216 h fter infetion tin d 9 8 ** * / GFP (-) / GFP (+) 7 / GFP-Mkp1 (+) Suessful ytokinesis Cytokinesis filure without ontrtile ring formtion Asission filure Supplementry Figure 9. Regultion of phosphoryltion in ultured heptoytes: phrmologil regultion nd lentivirus-medited Mkp1 trnsdution. () nd totl fter tretment with MEK inhiitor (U126) or MKP1 inhiitor (BCI) in heptoytes isolted from liver. Note tht dereses fter U126 tretment, nd inreses fter BCI tretment. (, ) nd totl fter the overexpression of GFP-leled Mkp1 to heptoytes y lentivirl vetor. Time-ourse imges of GFP expression fter the trnsdution with lentivirus rrying GFP-leled Mkp1 (Lenti-GFP- Mkp1) () Immunolots of Mkp1 from ultured heptoytes trnsdued with Lenti-GFP-Mkp1 or with ontrol lentivirus rrying GFP (Lenti-GFP). () Protein lystes were extrted from the heptoytes fter 192 h of virus infetion. (d) Quntifition of three types of ytokinesis fter the overexpression of Lenti-GFP-Mkp1 or Lenti-GFP in ultured heptoytes (/GFP(-), n = 27, /GFP(+) n = 29, /GFP-Mkp1(+), n = 189; 3 experiments for eh group). All vlues represent the men ± SEM. *P<., **P<.1, P<.1. Sle r, mm.
12 MKP Atin Atin 7 Atin d e f p/2 MAPK () pnakt 7 pmek1/2 7 7 Phospho-Akt (Thr38) Phospho-p/2 MAPK() (Thr22/2 /Thr18/187) 3 p8 7 Phospho-Akt (Ser73) Mek1/2 Atin Atin Atin IR 3 7 S6 Riosoml Protein MKP1 IRS1 17 Atin 3 Phospho-S6 Riosoml Protein (Ser23/236) 3 Supplementry Figure 1. Imges of unropped Western lots shown in Fig. (), Fig. (), Supplementry Fig. (), Supplementry Fig. 7 (d), Supplementry Fig. 9 (e), nd Supplementry Fig. 9 (f).
13 Supplementry Disussion Differene of expression in pluripotent perientrl stem ells (PCS) nd mture heptoytes surrounding PCS. It is interesting to note tht the Mkp1- pthwy ppers wek in perientrl stem ells (PCS) (Wng et l. 21) lining the entrl vein, ut more tive in mture surrounding PCS, whih re possile desendnts of PCS (Wng et l. 21). Sine Ki67-expressing ells, representing the ells in growth frtion (ells out of G ), were sprsely distriuted ut dominntly loted in the midloulr region (Supplementry Fig. 6g), the mture thus pper to ply entrl role in homeostti renewing proess of orgn mss, in ontrst to pluripotent PCS stem ells. The seprtion of pluripotent stem ells nd prolifertive ells is ommonly known in most tissues suh s smll intestine 1. Here, prolifertive heptoytes were dispersed mong silent G heptoytes (Supplementry Fig. 6g). Although orreltion etween rte of prolifertion nd liver polyploidiztion hs een reported 2, 27, 71, no suh orreltion ws found in liver (Supplementry Fig. 6i). Supplementry Referenes 71 Gupt, S. Hepti polyploidy nd liver growth ontrol. Semin Cner Biol. 1, (2)
SUPPLEMENTARY INFORMATION
DOI: 1.138/n358 TLR2 nd MyD88 expression in murine mmmry epithelil supopultions. CD24 min plus MRU Myo-epithelil Luminl progenitor (CD61 pos ) Mture luminl (CD61 neg ) CD49f CD61 Reltive expression Krt5
SUPPLEMENTARY INFORMATION
{ OI: 1.138/n31 Srifie n nlyze APs on week 1 s of iet 1 4 6 High-ft iet BrU High-ft iet BrU 4 High-ft iet BrU 6 High-ft iet BrU Lin - Lin - : C34 + : C9 + 1 1 3 1 4 1 5 C45 1 C34 1 1 1 1 3 1 4 1 5 S-1
Alimonti_Supplementary Figure 1. Pten +/- Pten + Pten. Pten hy. β-actin. Pten - wt hy/+ +/- wt hy/+ +/- Pten. Pten. Relative Protein level (% )
Alimonti_Supplementry Figure 1 hy 3 4 5 3 Neo 4 5 5 Proe 5 Proe hy/ hy/ /- - 3 6 Neo β-tin d Reltive Protein level (% ) 15 1 5 hy/ /- Reltive Gene Expr. (% ) 15 1 5 hy/ /- Supplementry Figure 1 Chrteriztion
SUPPLEMENTARY INFORMATION
doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy
Supplementary Figure S1
Supplementry Figure S1 - UTR m - 3HA - 2-1 hgh - 1 Uiquitin *! *! lk distl promoter m K3R/ K121R-3HA UTR hgh founder lines - HA - - founder lines TG- E1 L A2 B1 F9 G6 H4 H6 B C D2 G1 H3 J2 L - 7 IP: lk
SUPPLEMENTARY INFORMATION
DOI: 1.13/n7 Reltive Pprg mrna 3 1 1 Time (weeks) Interspulr Inguinl Epididyml Reltive undne..1.5. - 5 5-51 51-1 1-7 7 - - 1 1-1 Lipid droplet size ( m ) 1-3 3 - - - 1 1-1 1-1 1-175 175-3 3-31 31-5 >5
Supplementary Figure 1. Scheme of unilateral pyramidotomy used for detecting compensatory sprouting of intact CST axons.
() BDA 2 weeks fter Py () AAVs Cre or GFP t P1 BDA 2 weeks fter Py CSMN CST () Py t P7 or 2 months () Py t 2 months Supplementry Figure 1. Sheme of unilterl pyrmidotomy used for deteting ompenstory sprouting
Cos7 (3TP) (K): TGFβ1(h): (K)
IP#2: IP#1: Totl Lystes luiferse tivity (K): 6-4 - (K): luiferse tivity luiferse tivity (K): 2 1 RL-: - + + + + + Sm4-3F: + - + + + + MYC-Sm3: - - - - + + TβRI-HA(T204D): - - - + - + α-ha Luiferse Ativity
SUPPLEMENTARY INFORMATION
% ells with ili (mrke y A-Tu) Reltive Luiferse % ells with ili (mrke y Arl13) % ells with ili DOI: 1.138/n2259 A-Tuulin Hoehst % Cilite Non-ilite -Serum 9% 8% 7% 1 6% % 4% +Serum 1 3% 2% 1% % Serum: -
SUPPLEMENTARY INFORMATION
DOI: 1.138/n2977 Numer of ells per field 6 4 2 P =.1 Orthotopi eum Normlized ventrl photon flux 1E7 1E6 1E5 1E4 1E3 1E2 n=8 n=9 1 2 3 4 5 6 Dys Dy54 1.5E5 2.4E7 d Mie with lymph node metstsis (%) 1 8 6
SUPPLEMENTARY INFORMATION
DOI:.3/n95 Thymus Kiney (kd) TA T7 T TA T7 T Hert TA T7 T: +Dox Cylin B (kd) Thymus Kiney Hert TA T5 T TA T5 T TA T5 T: +Dox Cylin B Poneu S Poneu S CnB T7 CnB T Thymus (kd) + Liver Colon + + (kd) Thymus
LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb
SUPPLEMENTARY INFORMATION Supplementl Figure 1 doi:10.1038/nture09742 Lterl 1.0 mm from midline mpfc BNST mpfc BNST Lterl 2.1 mm from midline LHA LHA Lterl 2.7 mm from midline SUPPLEMENTAL INFORMATION
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:.8/nture89 4 4 Ilr -/- Ilr -/- Ilr -/- Cspse- -/- As -/- Nlrp -/- Il8 -/- Ilr -/- Supplementl figure. Inresed severity of NASH in inflmmsome-defiient mie, ut not in Ilr-defiient
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION CD169 + MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES Ptrii Brrl, Polo Polzell, Andres Brukuer, Nio vn Rooijen, Gurdyl S.
EFFECT OF DIETARY ENZYME ON PERFORMANCE OF WEANLING PIGS
EFFECT OF DIETARY ENZYME ON PERFORMANCE OF WEANLING PIGS Finl report sumitted to Dniso Animl Nutrition E. vn Heugten nd B. Frederik North Crolin Stte University, Deprtment of Animl Siene Summry The urrent
SUPPLEMENTARY INFORMATION
doi:1.138/nture1794 BR EPFs BRI1? ERECTA TMM BSKs YDA PP2A BSU1 BIN2 pbzr1/2 BZR1/2 MKK4/5/7/9 MPK3/6 SPCH Cell growth Stomtl production Supplementry Figure 1. The model of BR nd stomtl signling pthwys.
RESEARCH ARTICLE. Supplemental Figure 5
11.5 2 2 11. RESEARCH ARTICLE RBC ( 1 12 /L) 1.5 1. 9.5 PLT ( 1 9 /L) 1 16 14 HGB (g/l) 19 1 17 16 9. 12 4 4 46 Cellulr & Moleulr Immunology dvne online pulition, PCV (%) 44 MCV (fl) 46 44 ; doi:1.13/mi.214.16
P AND K IN POTATOES. Donald A Horneck Oregon State University Extension Service
P AND K IN POTATOES Donld A Hornek Oregon Stte University Extension Servie INTRODUCTION Phosphorous nd potssium re importnt to grow high yielding nd qulity pottoes. Muh of the northwest hs hd trditionlly
SUPPLEMENTARY INFORMATION
Prentl doi:.8/nture57 Figure S HPMECs LM Cells Cell lines VEGF (ng/ml) Prentl 7. +/-. LM 7. +/-.99 LM 7. +/-.99 Fold COX induction 5 VEGF: - + + + Bevcizum: - - 5 (µg/ml) Reltive MMP LM mock COX MMP LM+
SUPPLEMENTARY INFORMATION
doi:.8/nture98 : hr NEMO :5 hr IKK IKK NF-κB p65 p5 p65/-rel NF-κB p65 p5 p65/-rel Cytoplsm Cytoplsm p65/p5 Nuleus Nuleus NEMO IKK IKK d : hr > : hr p65/-rel NF- p65 p5 Cytoplsm Cytoplsm p65/p5 p65/-rel
DOI: 10.1038/nc2331 PCre;Ros26R 12 h induction 48 h induction Vegfr3 i EC c d ib4 24 h induction VEGFR3 e Fold chnge 1.0 0.5 P < 0.05 Vegfr3 i EC Vegfr3 Figure S1 Cre ctivtion leds to genetic deletion
Supplementary Information
Supplementry Informtion Non-nonil prevents skeletl ging n inflmmtion y inhiiting NF-κB Bo Yu, Ji Chng, Yunsong Liu, Jiong Li, Kreen Kevork, Khli Al Hezimi, Dn T. Grves, No-Hee Prk, Cun-Yu Wng Supplementry
Supplementary figure 1
Supplementry figure 1 Dy 8 post LCMV infection Vsculr Assoc. Prenchym Dy 3 post LCMV infection 1 5 6.7.29 1 4 1 3 1 2 88.9 4.16 1 2 1 3 1 4 1 5 1 5 1.59 5.97 1 4 1 3 1 2 21.4 71 1 2 1 3 1 4 1 5 1 5.59.22
SUPPLEMENTARY INFORMATION
doi:10.1038/nture10754 Supplementry note 1 To ompre our dt with previous studies, we mesured the width of spikes from identified dopminergi neurons nd unidentified neurons from DATCre mie. Previous studies
The Hippo/YAP pathway interacts with EGFR signaling and HPV oncoproteins to regulate cervical cancer progression
Reserh Artile The Hippo/ pthwy interts with EGFR signling nd HPV onoproteins to regulte ervil ner progression Chuno He 1,, Dgn Mo 1,3, Guohu Hu 1,, Xingmin Lv 1, Xingheng Chen, Peter C Angeletti 5, Jixin
Expression of Three Cell Cycle Inhibitors during Development of Adipose Tissue
Expression of Three Cell Cycle Inhiitors during Development of Adipose Tissue Jiin Zhng Deprtment of Animl Sciences Advisor: Michel E. Dvis Co-dvisor: Kichoon Lee Development of niml dipose tissue Hypertrophy
Acute and gradual increases in BDNF concentration elicit distinct signaling and functions in neurons
nd grdul increses in BDNF concentrtion elicit distinct signling nd functions in neurons Yunyun Ji,, Yun Lu, Feng Yng, Wnhu Shen, Tin Tze-Tsng Tng,, Linyin Feng, Shumin Dun, nd Bi Lu,.. - Grdul (normlized
SUPPLEMENTARY INFORMATION
DOI:./n BJ RAS:ER Herrnz et l Supplementry Figure HFFF RAS:ER.. mrna Expression..... ILα ILβ IL IL CCL INH VEGF mrna Expression..... ILα ILβ IL IL CCL INH VEGF + OHT Torin NVP-BEZ + OHT shmtor. shmtor.
Chow KD CR HFD. Fed Fast Refed
Supplementry Figure 1 Control d/d Chow KD CR Fed Fst Refed Supplementry Figure 1: Liver expression in diet nd disese models. () expression in the livers of ontrol nd d/d mie. () expression in the livers
Supplementary Figure 1
Supplementry Figure 1 c d Wistr SHR Wistr AF-353 SHR AF-353 n = 6 n = 6 n = 28 n = 3 n = 12 n = 12 Supplementry Figure 1 Neurophysiologicl properties of petrosl chemoreceptive neurones in Wistr nd SH rts.
SUPPLEMENTARY INFORMATION
. Norml Physiologicl Conditions. SIRT1 Loss-of-Function S1. Model for the role of SIRT1 in the regultion of memory nd plsticity. () Our findings suggest tht SIRT1 normlly functions in coopertion with YY1,
SUPPLEMENTARY INFORMATION
doi:.38/nture277 d 25 25 2 Time from sound onset (ms) 25 25 2 Time from sound onset (ms) Firing rte (spikes/s) Firing rte (spikes/s).8.6..2 e f g h.8.6..2 Frtion of neurons Frtion of neurons N = 53 2 2
SUPPLEMENTARY INFORMATION
2 weeks high holesterol diet 2 weeks high holesterol diet 2 weeks high holesterol diet 2 μm Mrophges Crystls Hoehst μm Mrophges Crystls Hoehst Hoehst Crystls Mrophges 2 μm 2 μm Supplementry Fig. 1: Erly
(% of adherent cells) *** PBL firm adhesion. Frequency (% ) 4 1 L 2 CXCR3 DP-2
Chemotxis (% of dded ells) PBL totl dhesion (N ells/mm 2 /1.1 6 PBL) Frequeny (% ) PBL firm dhesion Supplementry Figure 1 4 4 3 3 2 2 1.1-4 1-3 1.1.2. 1 1 8 6 4 2 Adiponetin ( g/ml) - + Adiponetin ( g/ml)
SUPPLEMENTARY INFORMATION
oi:1.138/nture1138 Supplementl Figure 1 Inflmmtory Monoytes Host ells CCR2 CCL2 Disseminting Tumor Cells Metstsis Assoite Mrophges VEGF Extrvstion & Metstti Seeing Supplementl Figure 1 The t from this
Copy Number ID2 MYCN ID2 MYCN. Copy Number MYCN DDX1 ID2 KIDINS220 MBOAT2 ID2
Copy Numer Copy Numer Copy Numer Copy Numer DIPG38 DIPG49 ID2 MYCN ID2 MYCN c DIPG01 d DIPG29 ID2 MYCN ID2 MYCN e STNG2 f MYCN DIPG01 Chr. 2 DIPG29 Chr. 1 MYCN DDX1 Chr. 2 ID2 KIDINS220 MBOAT2 ID2 Supplementry
TNF-α (pg/ml) IL-6 (ng/ml)
Xio, et l., Supplementry Figure 1 IL-6 (ng/ml) TNF-α (pg/ml) 16 12 8 4 1,4 1,2 1, 8 6 4 2 med Cl / Pm3CSK4 zymosn curdln Poly (I:C) LPS flgelin MALP-2 imiquimod R848 CpG TNF-α (pg/ml) IL-6 (ng/ml) 2 1.6
Supplementary information for: Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage
Supplementry informtion for: Low one mss nd chnges in the osteocyte network in mice lcking utophgy in the osteolst linege Mrilin Piemontese, Meld Onl, Jinhu Xiong, Li Hn, Jeff D. Thostenson, Mri Almeid,
PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis
Supplementry Informtion PDGF-BB secreted y preosteoclsts induces ngiogenesis during coupling with osteogenesis Hui Xie, Zhung Cui, Long Wng, Zhuying Xi, Yin Hu, Lingling Xin, Chngjun Li, Ling Xie, Jnet
GSK-3 is a master regulator of neural progenitor homeostasis
GSK-3 is mster regultor of neurl progenitor homeostsis Woo-Yng Kim 1, Xinshuo Wng 1, Yohong Wu 1, Brdley W Dole 2, Stish Ptel 3, Jmes R Woodgett 3 & Willim D Snider 1 The development of the rin requires
a3 Chains of type V collagen regulate breast tumour growth via glypican-1
Reeive 5 Aug 16 Aepte De 16 Pulishe 19 Jn 17 3 Chins of type V ollgen regulte rest tumour growth vi glypin-1 Guorui Hung 1, Goxing Ge 1,w, Vlerio Izzi & Dniel S. Greenspn 1 DOI: 1.138/nomms1351 OPEN Periellulr
SUPPLEMENTARY INFORMATION
DOI:.38/nc393 Nnog DAPI Nnog/DAPI c-jun DAPI c-jun/dapi c e Reltive expression to Gpdh mescs ( Feeder free) mescs ( Feeder) MEFs.5 MEFs ipscs ESCs..5 p=.24 p=.483 p=.22. JunB JunD Fos Fr Fr2 ATF2 ATF3
Supplementary Information
Supplementry Informtion A new lss of plnt lipid is essentil for protetion ginst phosphorus depletion Yozo Okzki 1, Hitomi Otsuki 1, Tomoko Nrisw 1, Mkoto Koyshi 1, Storu Swi 2, Yukiko Kmide 1, Miyko Kusno
Supplementary Figure S1
Supplementry Figure S Connexin4 TroponinI Merge Plsm memrne Met Intrcellulr Met Supplementry Figure S H9c rt crdiomyolsts cell line. () Immunofluorescence of crdic mrkers: Connexin4 (green) nd TroponinI
Protein tyrosine phosphatase 1B deficiency or inhibition delays ErbB2-induced mammary tumorigenesis and protects from lung metastasis
Protein tyrosine phosphtse 1B defiieny or inhiition delys ErB2-indued mmmry tumorigenesis nd protets from lung metstsis Sofi G Julien 1,5, Ndi Dué 1,6, Mihelle Red 1, Jnie Penney 1, Mrilene Pquet 2, Yongxin
SUPPLEMENTARY INFORMATION
DOI: 10.1038/nc2824 Hcn4 Tx5 Mlc2 c Hcn4- ISH d Tx5- ISH e Mlc2-ISH Hcn4-ISH f e Tx5-ISH f -ISH Figure S1 Section in situ hyridistion nlysis of crescent stge mouse emryos (E7.5). () More nterior section
Supplementary Figure S1_Cottini
Supplementry Figure S1_Cottini γ-h2a.x Krp OCIMy5 KMS11 Krps62 RPMI8226 INA6-1 µm Cleve C3 γ-h2a.x DAPI Merge OCIMy5 H929 JJN3 UTMC2 KMS11 KMS12PE KMS18 KMS2 RPMI8226 INA6 U266 KMS34 Krps62 1 2 3 4 5 6
Differential expression of cyclin G2, cyclin dependent kinase inhibitor 2C and peripheral myelin protein 22 genes during adipogenesis..
The Ohio Stte University From the SeletedWorks of Jiin Zhng Summer My 5, 214 Differentil expression of ylin G2, ylin dependent kinse inhiitor 2C nd peripherl myelin protein 22 genes during dipogenesis..pdf
Ulk λ PPase. 32 P-Ulk1 32 P-GST-TSC2. Ulk1 GST (TSC2) : Ha-Ulk1 : AMPK. WB: Ha (Ulk1) : Glu. h CON - Glu - A.A WB: LC3 AMPK-WT AMPK-DKO
DOI: 10.1038/ncb2152 C.C + - + - : Glu b Ulk1 - - + λ PPse c AMPK + - + + : ATP P-GST-TSC2 WB: Flg (Ulk1) WB Ulk1 WB: H (Ulk1) GST (TSC2) C.C d e WT K46R - + - + : H-Ulk1 : AMPK - + - + + + AMPK H-Ulk1
NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]
![NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]](/thumbs/92/110177253.jpg "NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]")
(A) W+OS 3 min 6 min con L S L S RNA loding control NppHS rrna (B) (C) 8 1 k NppHS reltive trnscript undnce 6 4.5 *** *** *** *** 3 k. + + + line 1 line (D) nicotine [µg mg -1 FM] 1..8.4. con W+W Supplementl
Supplementary Figure 1
doi: 1.138/nture6188 SUPPLEMENTARY INFORMATION Supplementry Figure 1 c CFU-F colonies per 1 5 stroml cells 14 12 1 8 6 4 2 Mtrigel plug Neg. MCF7/Rs MDA-MB-231 * * MCF7/Rs-Lung MDA-MB-231-Lung MCF7/Rs-Kidney
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nture11225 Numer of OTUs sed on 3% distnce Numer of 16s rrna-sed V2-V4 tg sequences LF MF PUFA Supplementry Figure 1. High-ft diets decrese the richness nd diversity
supplementary information
DOI:.38/n83 k Mouse Ch8 lous 8 9 Stop CHD8L 75 CHD8L Chromoomins Helise/ATPse omin DNA ining omin 5 kd NIH 3T3 MEF 93T HeL HCT UOS SOS.. CHD8L IB: CHD8 8 5 L S Reltive mrna mount 3... Reltive mrna mount.8.
SUPPLEMENTARY INFORMATION
SUPPEMENTARY INFORMATION DOI: 1.138/ncb956 Norml CIS Invsive crcinom 4 months months b Bldder #1 Bldder # Bldder #3 6 months (Invsive crcinom) Supplementry Figure 1 Mouse model of bldder cncer. () Schemtic
YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer
Li et l. Journl of Experimentl & Clinil Cner Reserh (7) 36:44 DOI.86/s346-7-6-3 RESEARCH Open Aess trnsriptionlly regultes expression nd GCCSysm-4 (G-4), dul / inhiitor, overomes drug resistne in oloretl
The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a camp-induced substrate switch
Reeived 6 Apr 216 Aepted 8 Sep 216 Pulished 22 Nov 216 DOI: 1.138/nomms13147 OPEN The GCN5-CITED2-PKA signlling module ontrols hepti gluose metolism through AMP-indued sustrte swith Mshito Ski 1, Tomoko
nestin ironetin p75 s1 CNS SKPs Dermo-1 +ve SKPs CNS H2O SCGs Skin Di. SKPs TH SHOX2 GAPDH NCAM D H Figure S1, Immunoytohemil nlysis o SKP spheres ulture rom neontl mouse (nestin, ironetin, S-1) or rt
Other Uses for Cluster Sampling
Other Uses for Cluster Smpling Mesure hnges in the level of n ttriute Hypothesis testing versus intervl estimtion Type I n 2 errors Power of the test Mesuring ttriute t sme time in ifferent sites Exmple:
Lesions of prefrontal cortex reduce attentional modulation of neuronal responses. and synchrony in V4
Lesions of prefrontl ortex reue ttentionl moultion of neuronl responses n synhrony in V4 Georgi G. Gregoriou,, Anrew F. Rossi, 3 Leslie G Ungerleier, 4 Roert Desimone 5 Deprtment of Bsi Sienes, Fulty of
*** *** *** *** T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. Relative ATP content. Relative ATP content RLU RLU
RLU Events 1 1 1 Luciferin (μm) T-cells T-ALL 1 1 Time (min) T-cells T-ALL 1 1 1 1 DCF-DA Reltive ATP content....1.1.. T-cells T-ALL RLU 1 1 T-cells T-ALL Luciferin (μm) 1 1 Time (min) c d Control e DCFH-DA
Inhibitory effect of p38 mitogen-activated protein kinase inhibitors on cytokine release from human macrophages
British Journl of Phrmology (26) 149, 393 44 & 26 Nture Pulishing Group All rights reserved 7 1188/6 $3. www.rjphrmol.org RESEARCH PAPER Inhiitory effet of p38 mitogen-tivted protein kinse inhiitors on
Ayman Hyder 1, Sabrina Ehnert 2, Hebke Hinz 1, Andreas K Nüssler 2, Fred Fändrich 1 and Hendrik Ungefroren 1,3*
Hyder et l. Cell Communition nd Signling 212, 1:23 http://www.biosignling.om/ontent/1/1/23 RESEARCH Open Aess EGF nd HB-EGF enhne the prolifertion of progrmmble ells of monoyti origin (PCMO) through tivtion
Interplay of LRRK2 with chaperone-mediated autophagy
Interply of with hperone-medited utophgy Smnth J Orenstein,, Sheng-Hn Kuo,, Inmuld Tsset,,, Espernz Aris,, Hiroshi Kog,, Irene Fernndez-Crs, Etty Cortes,5, Lwrene S Honig,5, Willim Duer 6, Antonell Consiglio,7,
Differences in metabolic and mitogenic signalling of insulin glargine and insulin aspart B10 in rats
Dietologi (13) 5:1 13 DOI 1.17/s15-13-93-z ARTICLE Differenes in metoli nd mitogeni signlling of insulin glrgine nd insulin sprt B1 in rts N. Tenngels & S. Welte & M. Hofmnn & P. Brenk & R. Shmidt & U.
SUPPLEMENTARY INFORMATION
X p -lu c ct ivi ty doi:.8/nture8 S CsA - THA + DAPI Merge FSK THA TUN Supplementry Figure : A. Ad-Xp luc ctivity in primry heptocytes exposed to FSK, THA, or TUN s indicted. Luciferse ctivity normlized
AJ PUTT. Hematology. Chemistry. Species: Canine Gender: Female Year of Birth: 2013 Client: PUTT
Speies: Cnine Gender: Femle Yer of Birth: 2013 Client: PUTT Requisition #: 9034-12 Aession #: W2152816 Aount Code: 72364 Veterinrin: CARTER Pnel/Profile: Tik Pnel Add-on Senior Profile with L 4Dx Plus
Supplementary Figure S1
Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus
SUPPLEMENTARY INFORMATION
doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.
Supplementary Figure 1
Roles of endoplsmic reticulum stress-medited poptosis in -polrized mcrophges during mycocteril infections Supplementry informtion Yun-Ji Lim, Min-Hee Yi, Ji-Ae Choi, Jung-hwn Lee, Ji-Ye Hn, Sung-Hee Jo,
RNAi Targeting CXCR4 Inhibits Tumor Growth Through Inducing Cell Cycle Arrest and Apoptosis
originl rtile RNAi Trgeting CXCR4 Inhiits Tumor Growth Through Induing Cell Cyle Arrest nd Apoptosis To Yu 1,2, Yingying Wu 2, Yi Hung 1,2, Chorn Yn 1, Ying Liu 1, Zongsheng Wng 3, Xioyi Wng 1, Yuming
Abortion frequency (%) Ovary position on ear Ovary volume (mm 3 )
ortion frequeny (%) 5 1 Ovry position on er 3 1 WW WD pex Bse Ovry volume (mm 3 ) Figure S1. Ovry volume (thik lines) n ortion frequeny (thin lines) s funtion of position long the er, 15 ys fter silk emergene
Imaging analysis of clock neurons reveals light buffers the wake-promoting effect of dopamine
Imging nlysis of lok neurons revels light uffers the wke-promoting effet of dopmine Yuhu Shng 1,2, Pul Hynes 2, Niolás Pírez 2, Kyle I Hrrington 3, Fng Guo 1,2, Jordn Pollk 3, Pengyu Hong 3, Leslie C Griffith
* * * * * liver kidney ileum. Supplementary Fig.S1
Supplementry Fig.S1 liver kidney ileum Fig.S1. Orlly delivered Fexrmine is intestinlly-restricted Mice received vehicle or Fexrmine (100mg/kg) vi per os (PO) or intrperitonel (IP) injection for 5 dys (n=3/group).
regulates stem cells through Wnt/β-catenin signalling
LETTERS O regultes stem ells through Wnt/β-tenin signlling Jolly Mzumdr,,7, W. Timothy O Brien, Rndll S. Johnson, Joseph C. LMnn, Jun C. Chvez 5, Peter S. Klein nd M. Celeste Simon,, Stem ells reside in
LETTERS. Chfr is required for tumor suppression and Aurora A regulation
25 Nture Pulishing Group http://www.nture.om/nturegenetis Chfr is required for tumor suppression nd Auror A regultion Xiohun Yu 1, Ktherine Minter-Dykhouse 1, Liviu Mlurenu 2, Wei-Meng Zho 3, Dongwei Zhng
Supplementary Information
Supplementry Informtion Cutneous immuno-surveillnce nd regultion of inflmmtion y group 2 innte lymphoid cells Ben Roediger, Ryn Kyle, Kwok Ho Yip, Nitl Sumri, Thoms V. Guy, Brin S. Kim, Andrew J. Mitchell,
SUPPLEMENTARY INFORMATION
TM TM tip link horizontl top connectors 1 leucine-rich (21 %) otoncorin-like 1809 ntigenic peptides B D signl peptide hydrophoic segment proline/threonine-rich (79 %) Supplementry Figure 1. () The outer
Effects of exercise training on hepatic steatosis in high fat diet-induced obese mice
Effets of exerise trining on hepti stetosis in high ft diet-indued oese mie Hyunsik Kng, PhD Sungkyunkwn University Non-Aloholi Ftty Liver Disese (NAFLD) A reversile ondition tht is hrterized y hepti lipid
Insulin-like Growth Factor-binding Protein-7 (IGFBP7): A Promising Gene Therapeutic for Hepatocellular Carcinoma (HCC)
originl rtile The Amerin Soiety of Gene & Cell Therpy Insulin-like Growth Ftor-inding Protein-7 (IGFBP7): A Promising Gene Therpeuti for Heptoellulr Crinom (HCC) Dong Chen 1, Ayesh Siddiq 2, Luni Emdd
Stable reprogrammed heterokaryons form spontaneously in Purkinje neurons after bone marrow transplant
Stle reprogrmmed heterokryons form spontneously in Purkinje neurons fter one mrrow trnsplnt Jmes M. Weimnn 1,2,Cls B. Johnsson 1,Angeli Trejo 1 nd Helen M. Blu 1,2 Heterokryons re the produt of ell fusion
Title of Experiment: Author, Institute and address:
Title of Experiment: Trsfetion of murine mrophge RAW264.7 ells with METAFECTENE PRO. Author, Institute n ress: Ptrizi Pellegtti n Frneso Di Virgilio. Deprtment of Experimentl n Dignosti Meiine, Setion
Rapamycin toxicity in MIN6 cells and rat and human islets is mediated by the inhibition of mtor complex 2 (mtorc2)
Dietologi (212) 55:1355 1365 DOI 1.17/s125-12-2475-7 ARTICLE myin toxiity in MIN6 ells nd rt nd humn islets is medited y the inhiition of mtor omplex 2 (mtorc2) A. D. Brlow & J. Xie & C. E. Moore & S.
SUPPLEMENTARY INFORMATION
DOI: 1.138/nc286 Figure S1 e f Medium DMSO AktVIII PP242 Rp S6K1-I Gr1 + + + + + + Strvtion + + + + + IB: Akt-pT38 IB: Akt K-pT389 K IB: Rptor Gr1 shs6k1-a shs6k1-b shs6k1-c shrictor shrptor Gr1 c IB:
Progestin effects on cell proliferation pathways in the postmenopausal mammary gland
Wood et l. Brest Cner Reserh 213, 15:R62 http://rest-ner-reserh.om/ontent/15/4/r62 RESEARCH ARTICLE Open Aess Progestin effets on ell prolifertion pthwys in the postmenopusl mmmry glnd Chrles E Wood 1,
Neural population coding of sound level adapts to stimulus statistics
COMPUTATION AND SYSTEMS 25 Nture Pulishing Group http://www.nture.om/ntureneurosiene Neurl popultion oding of sound level dpts to stimulus sttistis Isel Den 1, Niol S Hrper 1,2 & Dvid MAlpine 1 Mmmls n
Stimulation of the insulin/mtor pathway delays cone death in a mouse model of retinitis pigmentosa
Stimultion of the insulin/mtor pthwy delys one deth in mouse model of retinitis pigmentos Cludio Punzo 1, Krl Kornker 2 & Constne L Cepko 1,3 Retinitis pigmentos is n inurle retinl disese tht leds to lindness.
SUPPLEMENTARY INFORMATION
doi:1.138/nture17 Men tumour dimeter (mm) 2 Rg2-/- 2 1 2 2 1 Control IgG!-CD8!-CD4 1 2 3 1 2 3 c Men tumour dimeter (mm) 2 2 1 d Ifnr1-/- Rg2-/- 2 2 1 Ifngr1-/- d42m1!ic 1 2 3 Dys post trnsplnt 1 2 3 Supplementry
SUPPLEMENTARY INFORMATION
Supplementry Figure 1. Genertion of N- nd C-tgged cyclin D1 knock-in mice., N-tgged cyclin D1 gene trgeting construct, cyclin D1 genomic locus, cyclin D1 locus following homologous recomintion (trgeted
Supplemental Figures and Legends
Supplementl Figures n Legens Epigeneti trgeting of Hegehog trnsriptionl output through BET romoomin inhiition Yujie Tng 1,2, Shrreh Gholmin 2, Simone Shuert 1,2, Mine I. Willrson 3, Alex Lee 4, Prtiti
LKB1 and AMPK regulate synaptic remodeling in old age
LKB1 nd AMPK regulte synpti remodeling in old ge Melnie A Smuel 1,8, P Emnuel Voinesu 1,8, Brendn N Lilley 1, Rf de Co 2, Mr Foretz 3 5, Benoit Viollet 3 5, Bsil Pwlyk, Mihel A Snderg, Demetrios G Vvvs
EFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE
Swine Dy 22 Contents EFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE B. J. Johnson, J. P. Kyser, J. D. Dunn, A. T. Wyln, S. S. Dritz 1, J.
Chloride Nutrition Regulates Water Balance in Plants
XII Portuguese-Spnish Symposium on Plnt Wter Reltions Chloride Nutrition Regultes Wter Blne in Plnts Frno-Nvrro JD 1, Brumós J, Rosles MA 1, Vázquez-Rodríguez A 1, Sñudo BJ 1, Díz- Rued P 1, Rivero C 1,
Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota
Reeived 11 Jul 1 Aepted Fe Pulished 11 Mr DOI: 1.138/nomms795 OPEN Adipose tissue NAPE-PLD ontrols ft mss development y ltering the rowning proess nd gut miroiot Luie Geurts 1, Amndine Everrd 1,, Mtthis
SUPPLEMENTARY INFORMATION
~ 5 % ltion > 99 % ltion 25 2 15 5 Gly yemi (mmol/l) 3 7 15 3 Time fter -ell ltion (dys) Survivl (%) & ~ 5 % ltion 75 > 99% ltion 5 25 25 5 (dys) 7.4 d 1.25 lood ph 73 7.3 7.2 7.1 7. 7d ody weight h nge
supplementary information
DOI: 10.1038/nc2089 H3K4me1 H3K4me1 H3K4me1 H3K4me1 H3K4me1 H3K4me1 5 PN N1-2 PN H3K4me1 H3K4me1 H3K4me1 2-cell stge 2-c st cell ge Figure S1 Pttern of loclistion of H3K4me1 () nd () during zygotic development
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi: 1.138/nnno.211.41 Sili nd titnium dioxide nnoprtiles use pregnny omplitions in mie Kohei Ymshit, Ysuo Yoshiok, Kzum Higshisk, Kzuy Mimur, Yuki Morishit, Mstoshi Nozki, Tokuyuki
Activation of Akt as a Mechanism for Tumor Immune Evasion
The Amerin Soiety of Gene Therpy originl rtile Ativtion of Akt s Mehnism for Tumor Immune Evsion Kyung Hee Noh 1, Te Heung Kng 1, Jin Hee Kim 1, Sr I Pi 2, Ken Y Lin 3, Chien-Fu Hung 4, T-C Wu 4 7 nd Te
The microrna mir-31 inhibits CD8 + T cell function in chronic viral infection
A rt i l e s The mirorna mir-3 inhiits CD8 + T ell funtion in hroni virl infetion Howell F Moffett, Adm N R Crtwright, Hye-Jung Kim, Jernej Gode, Json Pyrdol, Trmo Äijö 3, Gustvo J Mrtinez,6, Anjn Ro,
Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells
Soni Hedgehog promotes prolifertion of Noth-dependent monoilited horoid plexus tumour ells Li Li, Ktie B. Grusm,2, Jun Wng 3, Melody P. Lun 4,5, Jsmin Ohli 6, Hrt G. W. Lidov 4, Moni L. Clihio 4, Erling
The MAPK signaling pathways comprise a conserved set of. ERK Signaling in the Pituitary Is Required for Female But Not Male Fertility
ORIGINL RESERCH ERK Signling in the Pituitry Is Required for Femle ut Not Mle Fertility Sturt P. liss, ndrew Miller, my M. Nvrtil, JinJun Xie, Sen P. MDonough, Ptrii J. Fisher, Gry E. Lndreth, nd Mrk S.