SUPPLEMENTARY INFORMATION

Size: px
Start display at page:

Download "SUPPLEMENTARY INFORMATION"

Transcription

1 DOI: /nc2824 Hcn4 Tx5 Mlc2 c Hcn4- ISH d Tx5- ISH e Mlc2-ISH Hcn4-ISH f e Tx5-ISH f -ISH Figure S1 Section in situ hyridistion nlysis of crescent stge mouse emryos (E7.5). () More nterior section level of the sme emryo s shown in Figure 1h (~4ss). Expression domins of Hcn4, Tx5 nd Mlc2 (ll shown in green) re predominntly overlpping, ut not with tht of (shown in red). () Corresponding section levels through n E7.5 emryo s shown elow in (c+d). (c-d) Doule in situ hyridistions. Blue stining: NBT/BCIP (BM Purple) nd rown stining: INT/BCIP. (c) Hcn4 (lue) nd (rown) re expressed in predominntly non-overlpping expression domins. (d) Tx5 (lue) nd (rown) re expressed in predominntly non-overlpping expression domins. Expression domins of Hcn4 nd Tx5 re mostly overlpping, when compring their expression on djcent sections. (e) Single fluorescence in situ hyridistions on djcent sections of n E7.5 (~4ss) emryo. (e-f) Expression of Mlc2, Hcn4 nd Tx5 (ll in green) re predominntly expressed within the presumptive FHF, in contrry to expression primrily in the SHF (red). (e -f ) Section levels corresponding to (e) nd (f), respectively. Scle r: 50µm. 1

2 Hcn4-ISH Tx5-ISH -ISH Tx5-ISH -IHC Tx5-ISH -IHC Tx5-ISH -IHC Figure S2 Section in situ hyridistion nd IHC nlysis of crescent stge emryo (E7.5). () Single in situ hyridistions (NBT/BCIP, BM Purple) on djcent sections of n E7.5 (~2-4ss) mouse emryo. Corresponding section levels re shown in pnel on left side. Expression domins of Hcn4 nd Tx5 re mostly overlpping in presumptive FHF, ut not with. However, in the posterior re of the FHF nd SHF domins there is n overlpping expression domin etween Tx5 nd visile (ornge rrows), ut not with Hcn4 (lck rrow), suggesting tht Hcn4 is more exclusive to the FHF thn Tx5. () Co-stining showing single fluorescent in situ hyridistion of Tx5 (green) nd ISL1 protein loction (red) on djcent sections of n E7.5 (~2-4ss) mouse emryo. The two domins re lrgely non-overlpping; however, there is some colocliztion visile in the most lterl sides of the splnchnic mesoderm (rrows). There re lso some res, where there is wek stining for ISL1 protein visile (sterix), overlpping with Tx5 mrna, ut the ISL1 protein does not co-loclize with the nucleus ut seems to e present within the cytoplsm, indicting tht it my not e functionl in these res. Scle r: 50µm. 2

3 -ISH Hcn4-ISH Tx5-ISH Nkx2.5-ISH Mlc2-ISH -ISH Hcn4-ISH Tx5-ISH Mlc2-ISH Figure S3 Section in situ hyridistion nlysis of n erly hert tue stge emryo (E8.0). () Single colour in situ hyridistions on djcent sections. Corresponding section level s shown in drwing. Hcn4, Tx5, Mlc2 nd Nkx2.5 re expressed within the primitive hert tue, unlike, which is expressed in the phryngel mesoderm. At this developmentl stge, Nkx2.5 is mostly non-overlpping with expression. () Single fluorescent in situ hyridistions on djcent sections. Corresponding section levels re shown in drwings ove imges. Expression domins of Hcn4, Tx5 nd Mlc2 (ll green) re mostly overlpping, ut predominntly non-overlpping with expression (red). However, posterior to the primitive hert tue, there is n re of overlp etween Tx5 nd expression (rrow), ut not with Hcn4 nd Mlc2 (most likely no overlp with, s the section level is it more nterior compred to (djcent sections) nd it seems more lterl compred to ). Scle r: 50µm. 3

4 Hcn4 CreErt2/+ ;R26R lcz Tmoxifen: E8.0 Hcn4 CreErt2/+ ;R26R eyfp Tmoxifen: E7.0 c Hcn4 CreErt2/+ ;R26R lcz Tmoxifen: E7.0 dorsl ventrl d e f Hcn4 CreErt2/+ ;R26R lcz Tmoxifen: E7.0 E19.5 E19.5 E19.5 Hcn4 CreErt2/+ ;R26R lcz Tmoxifen: E7.0 ISH E12.5 ventrl -ISH dorsl g Tx5-ISH Hcn4-ISH E10.5 E9.5 E10.5 E9.5 HCN4 CreErt2/+ ;R26R eyfp ; Tmoxifen: E7.0; E19.5 Atri h m-tnnt2- Alex647 Mlc2-ISH tdtomto+ 14% tdtomto+ 12.7% tdtomto+ 14.7% tomto+/tnnt2 82% YFP TNNT2 mgnified HCN4 CreErt2/+ ;R26R eyfp ; Tmoxifen: E7.0; E19.5 Atri PECAM-1-APC tomto+/ PECAM-1 0.1% r-smmhc-alex647 tomto+/ smmhc 1.2% FITC-A FITC-A FITC-A Figure S4 Hcn4+/FHF linege trcing in the emryonic hert nd Hcn4+/ FHF cell linege contriution to tri () Whole-mount X-gl stining of Hcn4 CreErt2/+ ;R26R lcz herts t E19.5. Tmoxifen induced Hcn4 CreErt2/+ ctivtion t E8.0 lels the Hcn4+/FHF derivtives, such s the left ventricle (LV) nd prts of the tri. At this stge, lso the sinus venosus (SV) nd sinotril node (SAN) re stined positive. () Section through n E19.5 Hcn4 CreErt2/+ ;R26R eyfp hert; Hcn4 CreErt2/+ ctivtion y tmoxifen t E7.0. Within the left ventricle, there is Hcn4+/FHF linege contriution to the treculr nd compct lyer of the left ventricle, nd some minor contriution to the septum. (c-d) Whole-mount X-gl stining of Hcn4 CreErt2/+ ;R26R lcz hert t E12.5 (c), nd emryos t E9.5 (d), nd E10.5 (e). Tmoxifen induced Hcn4 CreErt2/+ ctivtion t E7.0 lels the Hcn4+/FHF derivtives, such s the left ventricle (LV) nd prts of the tri. No stining is visile within the phryngel mesoderm (rrows, d+e). In situ hyridistion for of E9.5 emryo mrks the phryngel mesoderm (rrow, f). Hcn4+/FHF cells lso contriute to the AV cnl (red sterix, d+e). (g) Section through n E19.5 tri of Hcn4 CreErt2/+ ;R26R eyfp mice; Hcn4 CreErt2/+ ctivtion y tmoxifen t E7.0. Co-immunostining for YFP s mrker for Hcn4+/FHF linege trced cells nd TNNT2 shows contriution to crdiomyocytes. Yellow ox represents mgnified re shown in merge imge (h) FACS quntifiction of the cellulr contriution of Hcn4+/FHF linege trced cells to n Hcn4 CreErt2/+ ;R26R tdtomto E19.5 tri (Tmoxifen E7.0). FACS nlysis of tomto+ Hcn4+/FHF linege trced cells co-stined for TNNT2, PECAM-1 (CD31) or smmhc (MYH11) showed predominnt contriution to crdiomyocytes, ut no doule positive cells for endothelil/endocrdil-, nd vsculr smooth muscle mrkers, suggesting no contriution to these cell lineges within tri. Representtive FACS dot-plots re shown. Secondry ntiody controls re shown in Suppl. Fig. S7. Scle r: (+c) 0.5mm, () 100µm, (d-f) 0.2mm, (g) 50µm. 4

5 Hcn4 CreErt2/+ ;R26R eyfp ; no Tmoxifen EB dy7 dpi PE A-empty GFP-A yfp+ 0.0% Hcn4 CreErt2/+ ;R26R eyfp ;Tmoxifen EB dy 4+6 PE A-empty EB dy7 dpi yfp+ 2.1% c d Hcn4 Tx5 Nxk2.5 Tnnt2 Mlc2v Cx40 Srcolipin et-ctin Hcn4 CreErt2/+ ;R26R eyfp TNNT H2O H2O Hcn4 CreErt2/+ ;R26R eyfp TNNT2 0mV 0mV 0mV 50mV e Right Ventricle GFP-A 200ms ventriculr-like AP 64% TgAHF-Mef2c-eGFP; E19.5 tril-like AP 16% nodl-like AP 20% % m-tnnt2- Alex % r-smmhc-apc 1.8% 0.4% 29.6 PECAM-1-APC 25.3 Outflow trct, no tri m-tnnt2- Alex % 1.1% 13.2 r-smmhc-apc % 14.0 PECAM-1-APC 3.2% 1.0% 9.8% Figure S5 Clonl nlysis of Hcn4+/FHF cells isolted from mouse ESCs nd TgMef2c-AHF-eGFP/SHF cell linege contriution in vivo. () Emryonic stem cells (ECSs) were derived from Hcn4 CreErt2/+ ;R26R eyfp mice. Mouse ESCs were differentited using the emryoid ody technique. Hcn4 CreErt2/+ ctivity ws induced y dministrtion of 4-OH-Tmoxifen on dy 4 nd 6 of differentition. eyfp positive Hcn4+/FHF cells were FACS sorted (representtive FACS dot-plots re shown). () Single eyfp positive Hcn4+/FHF cells were sorted s 1 cell/well into 96-well pltes. After 2-3 weeks single cell derived clones showed limited expnsion nd efficient differentition into crdiomyogenic clones, visulized y direct fluorescence of eyfp nd immunostining for the crdic muscle mrker TNNT2 (ctnt). (c) RT-PCR nlysis, showing expression of crdic mrker genes in single Hcn4+/FHF cell derived crdiomyogenic clones. (d) Single cell ptch clmp nlysis (n=39). Most clmped cells showed ventriculr-like ction potentil (AP) (64%), while some showed more tril-like AP (16%), or nodl-like AP (20%). Representtive ction potentils re shown. (e) FACS quntifiction of the cellulr contriution of TgMef2c-AHF-eGFP/SHF linege trced cells to the right ventricle nd outflowtrct (OFT). Fixed single cells were co-stined for TNNT2, demonstrting contriution to crdiomyogenic cell lineges, prticulrly within the RV. Co-stining for smmhc (MYH11) showed minor contriution to vsculr smooth muscle cells in RV nd contriution in the OFT. Co-stining for PECAM-1 (CD31) showed minor contriution to endothelil cell linege in RV nd contriution in the OFT. Representtive FACS dot-plots re shown. Scle r: 50µm. 5

6 Hcn4 Hcn4 Nkx2.5 Cx40 Cx Tx5 Mlc2v ctnnt2 Srcolipin et-ctin ckground signl of secondry ntiodies single cells 2.8% single cells single cells 2.1% APC - empty g--m-alex647 APC - empty g--r-alex % 0.2% g--m-alex488 FITC - empty g--r-alex488 FITC - empty Figure S6 Uncropped key electrophoresis dt nd secondry ntiody controls of FACS nlyses. () Uncropped electrophoresis dt of RT-PCR shown in Figure 4c. () Bckground signl of secondry ntiodies used for FACS sorting s shown in Fig. 3f nd Suppl. Fig. 4h. 6

7 Video S1 Clonl nlysis of single Hcn4+/FHF cells isolted from mouse emryos. Representtive exmple of eting crdiomyogenic clone derived from single Hcn4+/FHF cell from mouse emryos. 7

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPEMENTARY INFORMATION DOI: 1.138/ncb956 Norml CIS Invsive crcinom 4 months months b Bldder #1 Bldder # Bldder #3 6 months (Invsive crcinom) Supplementry Figure 1 Mouse model of bldder cncer. () Schemtic

More information

DOI: 10.1038/nc2331 PCre;Ros26R 12 h induction 48 h induction Vegfr3 i EC c d ib4 24 h induction VEGFR3 e Fold chnge 1.0 0.5 P < 0.05 Vegfr3 i EC Vegfr3 Figure S1 Cre ctivtion leds to genetic deletion

More information

Acute and gradual increases in BDNF concentration elicit distinct signaling and functions in neurons

Acute and gradual increases in BDNF concentration elicit distinct signaling and functions in neurons nd grdul increses in BDNF concentrtion elicit distinct signling nd functions in neurons Yunyun Ji,, Yun Lu, Feng Yng, Wnhu Shen, Tin Tze-Tsng Tng,, Linyin Feng, Shumin Dun, nd Bi Lu,.. - Grdul (normlized

More information

Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome

Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome correction notice Nt. Med. 17, 726 731 (2011) Defective Wnt-dependent cereellr midline fusion in mouse model of Jouert syndrome Mdeline A Lncster, Dipik J Gopl, Joon Kim, Shr N Sleem, Jennifer L Silhvy,

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:0.08/nture0987 SUPPLEMENTARY FIGURE Structure of rbbit Xist gene. Exons re shown in boxes with romn numbers, introns in thin lines. Arrows indicte the locliztion of primers used for mplifiction. WWW.NATURE.COM/NATURE

More information

supplementary information

supplementary information DOI: 10.1038/nc2089 H3K4me1 H3K4me1 H3K4me1 H3K4me1 H3K4me1 H3K4me1 5 PN N1-2 PN H3K4me1 H3K4me1 H3K4me1 2-cell stge 2-c st cell ge Figure S1 Pttern of loclistion of H3K4me1 () nd () during zygotic development

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI:.38/nc393 Nnog DAPI Nnog/DAPI c-jun DAPI c-jun/dapi c e Reltive expression to Gpdh mescs ( Feeder free) mescs ( Feeder) MEFs.5 MEFs ipscs ESCs..5 p=.24 p=.483 p=.22. JunB JunD Fos Fr Fr2 ATF2 ATF3

More information

Supplementary figure 1

Supplementary figure 1 Supplementry figure 1 Dy 8 post LCMV infection Vsculr Assoc. Prenchym Dy 3 post LCMV infection 1 5 6.7.29 1 4 1 3 1 2 88.9 4.16 1 2 1 3 1 4 1 5 1 5 1.59 5.97 1 4 1 3 1 2 21.4 71 1 2 1 3 1 4 1 5 1 5.59.22

More information

Expression of Three Cell Cycle Inhibitors during Development of Adipose Tissue

Expression of Three Cell Cycle Inhibitors during Development of Adipose Tissue Expression of Three Cell Cycle Inhiitors during Development of Adipose Tissue Jiin Zhng Deprtment of Animl Sciences Advisor: Michel E. Dvis Co-dvisor: Kichoon Lee Development of niml dipose tissue Hypertrophy

More information

PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis

PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis Supplementry Informtion PDGF-BB secreted y preosteoclsts induces ngiogenesis during coupling with osteogenesis Hui Xie, Zhung Cui, Long Wng, Zhuying Xi, Yin Hu, Lingling Xin, Chngjun Li, Ling Xie, Jnet

More information

Supplementary information for: Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage

Supplementary information for: Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage Supplementry informtion for: Low one mss nd chnges in the osteocyte network in mice lcking utophgy in the osteolst linege Mrilin Piemontese, Meld Onl, Jinhu Xiong, Li Hn, Jeff D. Thostenson, Mri Almeid,

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementry Figure 1 c d Wistr SHR Wistr AF-353 SHR AF-353 n = 6 n = 6 n = 28 n = 3 n = 12 n = 12 Supplementry Figure 1 Neurophysiologicl properties of petrosl chemoreceptive neurones in Wistr nd SH rts.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION . Norml Physiologicl Conditions. SIRT1 Loss-of-Function S1. Model for the role of SIRT1 in the regultion of memory nd plsticity. () Our findings suggest tht SIRT1 normlly functions in coopertion with YY1,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:1.138/nture1794 BR EPFs BRI1? ERECTA TMM BSKs YDA PP2A BSU1 BIN2 pbzr1/2 BZR1/2 MKK4/5/7/9 MPK3/6 SPCH Cell growth Stomtl production Supplementry Figure 1. The model of BR nd stomtl signling pthwys.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:.3/nture93 d 5 Rttlesnke DRG (reds) Rttlesnke TG (reds) c 3 TRPV1 other TRPs 1 1 3 Non-pit snke TG (reds) SFig. 1 5 5 3 other TRPs TRPV1 1 1 3 Non-pit snke DRG (reds) 5 Antomy of the pit orgn nd comprison

More information

LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb

LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb SUPPLEMENTARY INFORMATION Supplementl Figure 1 doi:10.1038/nture09742 Lterl 1.0 mm from midline mpfc BNST mpfc BNST Lterl 2.1 mm from midline LHA LHA Lterl 2.7 mm from midline SUPPLEMENTAL INFORMATION

More information

Control vector. HA-Elfn2. HA-Elfn1

Control vector. HA-Elfn2. HA-Elfn1 Control vector c HA-Elfn2 HA- Control vector HA-Elfn2 HA- nti- nti-ha Hippocmpus (CA3) PV DAPI SOM DAPI Hippocmpus (dentte gyrus) PV DAPI SOM DAPI Supplementry Figure 1. Specificity of the nti- ntiody

More information

Supplementary Figure 1. Scheme of unilateral pyramidotomy used for detecting compensatory sprouting of intact CST axons.

Supplementary Figure 1. Scheme of unilateral pyramidotomy used for detecting compensatory sprouting of intact CST axons. () BDA 2 weeks fter Py () AAVs Cre or GFP t P1 BDA 2 weeks fter Py CSMN CST () Py t P7 or 2 months () Py t 2 months Supplementry Figure 1. Sheme of unilterl pyrmidotomy used for deteting ompenstory sprouting

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMEARY IFORMAIO doi:./nture correction to Supplementry Informtion Adenom-linked rrier defects nd microil products drive IL-/IL-7-medited tumour growth Sergei I. Grivennikov, Kepeng Wng, Dniel Mucid,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Synthesis of ONC-101 nd Derivtives: The imidzo-pyrzine Synthesis The synthesis of imidzo[1,2-]pyrzines is simple three-component-one-pot rection s shown in Scheme 1: Scheme 1: The synthesis of imidzo[1,2-]pyrzines

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Prentl doi:.8/nture57 Figure S HPMECs LM Cells Cell lines VEGF (ng/ml) Prentl 7. +/-. LM 7. +/-.99 LM 7. +/-.99 Fold COX induction 5 VEGF: - + + + Bevcizum: - - 5 (µg/ml) Reltive MMP LM mock COX MMP LM+

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Suppl. Fig. 1 in vivo expression of ISL1 in the human fetal heart. a, Hematoxylin eosin staining showing structures of left atrium and left atrium appendage (*) of a human fetal heart at 11 weeks of gestation.

More information

Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm

Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm Adipocyte in vsculr wll cn induce the rupture of dominl ortic neurysm Hiron Kugo 1 *, Nouhiro Zim 1 *, Hiroki Tnk 2 *, Youhei Mouri 1, Kenichi Yngimoto 3, Kohsuke Hymizu 3,4, Keisuke Hshimoto 1, Tkeshi

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 1.138/n358 TLR2 nd MyD88 expression in murine mmmry epithelil supopultions. CD24 min plus MRU Myo-epithelil Luminl progenitor (CD61 pos ) Mture luminl (CD61 neg ) CD49f CD61 Reltive expression Krt5

More information

The Dynamics of Varicella-Zoster Virus Epithelial Keratitis in Herpes Zoster Ophthalmicus

The Dynamics of Varicella-Zoster Virus Epithelial Keratitis in Herpes Zoster Ophthalmicus Chpter 2 The Dynmics of Vricell-Zoster Virus Epithelil Kertitis in Herpes Zoster Ophthlmicus The morphology of n individul VZV lesion reflects sequence of events triggered y the virus impct on cornel epithelil

More information

% Inhibition of MERS pseudovirus infection. 0 h 0.5 h 1 h 2 h 4 h 6 h Time after virus addition

% Inhibition of MERS pseudovirus infection. 0 h 0.5 h 1 h 2 h 4 h 6 h Time after virus addition % Inhiition of MERS pseudovirus infection 1 8 h.5 h 1 h 2 h 4 h 6 h Time fter virus ddition Supplementry Figure S1. Inhiition of on MERS pseudovirus infection t the different intervls postinfection. A

More information

CONCURRENT VASCULOGENESIS AND ANGIOGENESIS IN THE NORMAL HUMAN EMBRYO

CONCURRENT VASCULOGENESIS AND ANGIOGENESIS IN THE NORMAL HUMAN EMBRYO ORIGINAL ARTICLES CONCURRENT VASCULOGENESIS AND ANGIOGENESIS IN THE NORMAL HUMAN EMBRYO Anc Cimpen 1, Johnnes Achench 2, Mrius Ric 1 ABSTRACT Four humn emryos were investigted (5, 7, 7 nd 8 weeks old).

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:1.138/nture1228 Totl Cell Numer (cells/μl of lood) 12 1 8 6 4 2 d Peripherl Blood 2 4 7 Time (d) fter nti-cd3 i.p. + TCRβ + IL17A + cells (%) 7 6 5 4 3 2 1 Totl Cell Numer (x1 3 ) 8 7 6 5 4 3 2 1 %

More information

Supplementary Information. SAMHD1 Restricts HIV-1 Infection in Resting CD4 + T Cells

Supplementary Information. SAMHD1 Restricts HIV-1 Infection in Resting CD4 + T Cells Supplementry Informtion SAMHD Restricts HIV- Infection in Resting CD T Cells Hnn-Mri Blduf,2,, Xioyu Pn,, Elin Erikson,2, Srh Schmidt, Wqo Dddch 3, Mnj Burggrf, Kristin Schenkov, In Amiel,2, Guido Wnitz

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION TM TM tip link horizontl top connectors 1 leucine-rich (21 %) otoncorin-like 1809 ntigenic peptides B D signl peptide hydrophoic segment proline/threonine-rich (79 %) Supplementry Figure 1. () The outer

More information

TNF-α (pg/ml) IL-6 (ng/ml)

TNF-α (pg/ml) IL-6 (ng/ml) Xio, et l., Supplementry Figure 1 IL-6 (ng/ml) TNF-α (pg/ml) 16 12 8 4 1,4 1,2 1, 8 6 4 2 med Cl / Pm3CSK4 zymosn curdln Poly (I:C) LPS flgelin MALP-2 imiquimod R848 CpG TNF-α (pg/ml) IL-6 (ng/ml) 2 1.6

More information

Heart Development. Robert G. Kelly Developmental Biology Institute of Marseilles - Luminy

Heart Development. Robert G. Kelly Developmental Biology Institute of Marseilles - Luminy ESC CBCS Summer School on Cardiovascular Sciences 15th June 2011 Heart Development Robert G. Kelly Developmental Biology Institute of Marseilles - Luminy Animal models of heart development Tinman/Nkx2.5

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 1.13/n7 Reltive Pprg mrna 3 1 1 Time (weeks) Interspulr Inguinl Epididyml Reltive undne..1.5. - 5 5-51 51-1 1-7 7 - - 1 1-1 Lipid droplet size ( m ) 1-3 3 - - - 1 1-1 1-1 1-175 175-3 3-31 31-5 >5

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION % ells with ili (mrke y A-Tu) Reltive Luiferse % ells with ili (mrke y Arl13) % ells with ili DOI: 1.138/n2259 A-Tuulin Hoehst % Cilite Non-ilite -Serum 9% 8% 7% 1 6% % 4% +Serum 1 3% 2% 1% % Serum: -

More information

Supplementary Information

Supplementary Information Supplementry Informtion Cutneous immuno-surveillnce nd regultion of inflmmtion y group 2 innte lymphoid cells Ben Roediger, Ryn Kyle, Kwok Ho Yip, Nitl Sumri, Thoms V. Guy, Brin S. Kim, Andrew J. Mitchell,

More information

nestin ironetin p75 s1 CNS SKPs Dermo-1 +ve SKPs CNS H2O SCGs Skin Di. SKPs TH SHOX2 GAPDH NCAM D H Figure S1, Immunoytohemil nlysis o SKP spheres ulture rom neontl mouse (nestin, ironetin, S-1) or rt

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION X p -lu c ct ivi ty doi:.8/nture8 S CsA - THA + DAPI Merge FSK THA TUN Supplementry Figure : A. Ad-Xp luc ctivity in primry heptocytes exposed to FSK, THA, or TUN s indicted. Luciferse ctivity normlized

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S Connexin4 TroponinI Merge Plsm memrne Met Intrcellulr Met Supplementry Figure S H9c rt crdiomyolsts cell line. () Immunofluorescence of crdic mrkers: Connexin4 (green) nd TroponinI

More information

Heart Development. Origins of congenital heart defects Properties of cardiac progenitor cells. Robert G. Kelly

Heart Development. Origins of congenital heart defects Properties of cardiac progenitor cells. Robert G. Kelly ESC CBCS Summer School on Cardiovascular Sciences Heart Development 19th June 2013 Origins of congenital heart defects Properties of cardiac progenitor cells Robert G. Kelly Animal models of heart development

More information

A rt i c l e s. a Events (% of max)

A rt i c l e s. a Events (% of max) Continuous requirement for the TCR in regultory T cell function Andrew G Levine 1,, Aron Arvey 1,,4, Wei Jin 1,,4 & Alexnder Y Rudensky 1 3 14 Nture Americ, Inc. All rights reserved. Foxp3 + regultory

More information

Copy Number ID2 MYCN ID2 MYCN. Copy Number MYCN DDX1 ID2 KIDINS220 MBOAT2 ID2

Copy Number ID2 MYCN ID2 MYCN. Copy Number MYCN DDX1 ID2 KIDINS220 MBOAT2 ID2 Copy Numer Copy Numer Copy Numer Copy Numer DIPG38 DIPG49 ID2 MYCN ID2 MYCN c DIPG01 d DIPG29 ID2 MYCN ID2 MYCN e STNG2 f MYCN DIPG01 Chr. 2 DIPG29 Chr. 1 MYCN DDX1 Chr. 2 ID2 KIDINS220 MBOAT2 ID2 Supplementry

More information

Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome

Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome Supplementry Informtion Microtuule-driven sptil rrngement of mitochondri promotes ctivtion of the NLRP3 inflmmsome Tkum Misw 1,2, Michihiro Tkhm 1,2, Ttsuy Kozki 1,2, Hnn Lee 1,2, Jin Zou 1,2, Ttsuy Sitoh

More information

Supplementary Materials. Viral delivery of mir-196a ameliorates the SBMA phenotype via the silencing of CELF2

Supplementary Materials. Viral delivery of mir-196a ameliorates the SBMA phenotype via the silencing of CELF2 Supplementry Mterils Virl delivery of mir-96 meliortes the SBMA phenotype vi the silencing of CELF2 Yu Miyzki, Hiroki Adchi, Mshis Ktsuno, Mkoto Minmiym, Yue-Mei Jing, Zhe Hung, Hideki Doi, Shinjiro Mtsumoto,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nture09663 Scrmle shnlrp3 shcsp1 IL-1β (p17) IL-1β (pg/ml) 2000 1500 1000 500 Wt Nlrp3-/- Ipf-/- 0 APDC IL-1β (p17) Supplementl Figure 1. Mitochondril ROS cn trigger NLRP3 inflmmsome ctivtion,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION { OI: 1.138/n31 Srifie n nlyze APs on week 1 s of iet 1 4 6 High-ft iet BrU High-ft iet BrU 4 High-ft iet BrU 6 High-ft iet BrU Lin - Lin - : C34 + : C9 + 1 1 3 1 4 1 5 C45 1 C34 1 1 1 1 3 1 4 1 5 S-1

More information

*** *** *** *** T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. Relative ATP content. Relative ATP content RLU RLU

*** *** *** *** T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. Relative ATP content. Relative ATP content RLU RLU RLU Events 1 1 1 Luciferin (μm) T-cells T-ALL 1 1 Time (min) T-cells T-ALL 1 1 1 1 DCF-DA Reltive ATP content....1.1.. T-cells T-ALL RLU 1 1 T-cells T-ALL Luciferin (μm) 1 1 Time (min) c d Control e DCFH-DA

More information

NIH Public Access Author Manuscript Science. Author manuscript; available in PMC 2010 July 2.

NIH Public Access Author Manuscript Science. Author manuscript; available in PMC 2010 July 2. NIH Public Access Author Manuscript Published in final edited form as: Science. 2009 October 16; 326(5951): 426 429. doi:10.1126/science.1177350. Generation of Functional Ventricular Heart Muscle from

More information

% cells forming Neurospheres 81 ± 6 % 0 % 2.6 ± 0.7 % 76 ± 8 % 0 % 3.4 ± 0.6 % 83 ± 5 % 0 % 2.4 ± 0.9 % 89 ± 5 % 3 ± 1.5 % Total 10, ± 6 % 0 %

% cells forming Neurospheres 81 ± 6 % 0 % 2.6 ± 0.7 % 76 ± 8 % 0 % 3.4 ± 0.6 % 83 ± 5 % 0 % 2.4 ± 0.9 % 89 ± 5 % 3 ± 1.5 % Total 10, ± 6 % 0 % Bo et l., Suppl. Tle 1 Supplementl Tle 1. Neurosphere formtion nd tumorigencity is enriched within the tumour cell popultions derived from humn primry glioms nd gliom xenogrfts. GBM smples or Gliom xenogrfts

More information

COPYRIGHTED MATERIAL. Anatomical and Electrophysiological Bases CHAPTER The h eart w alls The s pecific c onduction s ystem (Fig. 1.

COPYRIGHTED MATERIAL. Anatomical and Electrophysiological Bases CHAPTER The h eart w alls The s pecific c onduction s ystem (Fig. 1. CHPTER ntomicl nd Electrophysiologicl ses.. The h ert w lls The hert hs four cvities, two tri nd two ventricles, comprised minly of contrctile cells clled crdiomyocytes. The electricl stimulus originting

More information

Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T- lymphocytes

Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T- lymphocytes Supporting Online Mteril for Heprnse promotes tumor infiltrtion nd ntitumor ctivity of -redirected T- lymphocytes IgnzioCrun, Brr Svoldo, VlentinHoyos, Gerrit Weer, Ho Liu, Eugene S. Kim, Michel M. Ittmnn,

More information

VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling

VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling R T I C L E S EGFR-3 controls tip to stlk conversion t vessel fusion sites y reinforcing Notch signlling Tuoms Tmmel 1,9, Georgi Zrkd 1,9, Hrri Nurmi 1, Lrs Jkosson 2,10, Krist Heinolinen 1, Denis Tvorogov

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION 2 weeks high holesterol diet 2 weeks high holesterol diet 2 weeks high holesterol diet 2 μm Mrophges Crystls Hoehst μm Mrophges Crystls Hoehst Hoehst Crystls Mrophges 2 μm 2 μm Supplementry Fig. 1: Erly

More information

Alimonti_Supplementary Figure 1. Pten +/- Pten + Pten. Pten hy. β-actin. Pten - wt hy/+ +/- wt hy/+ +/- Pten. Pten. Relative Protein level (% )

Alimonti_Supplementary Figure 1. Pten +/- Pten + Pten. Pten hy. β-actin. Pten - wt hy/+ +/- wt hy/+ +/- Pten. Pten. Relative Protein level (% ) Alimonti_Supplementry Figure 1 hy 3 4 5 3 Neo 4 5 5 Proe 5 Proe hy/ hy/ /- - 3 6 Neo β-tin d Reltive Protein level (% ) 15 1 5 hy/ /- Reltive Gene Expr. (% ) 15 1 5 hy/ /- Supplementry Figure 1 Chrteriztion

More information

Meat and Food Safety. B.A. Crow, M.E. Dikeman, L.C. Hollis, R.A. Phebus, A.N. Ray, T.A. Houser, and J.P. Grobbel

Meat and Food Safety. B.A. Crow, M.E. Dikeman, L.C. Hollis, R.A. Phebus, A.N. Ray, T.A. Houser, and J.P. Grobbel Met nd Food Sfety Needle-Free Injection Enhncement of Beef Strip Loins with Phosphte nd Slt Hs Potentil to Improve Yield, Tenderness, nd Juiciness ut Hrm Texture nd Flvor B.A. Crow, M.E. Dikemn, L.C. Hollis,

More information

NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]

NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM] (A) W+OS 3 min 6 min con L S L S RNA loding control NppHS rrna (B) (C) 8 1 k NppHS reltive trnscript undnce 6 4.5 *** *** *** *** 3 k. + + + line 1 line (D) nicotine [µg mg -1 FM] 1..8.4. con W+W Supplementl

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 1.138/nc286 Figure S1 e f Medium DMSO AktVIII PP242 Rp S6K1-I Gr1 + + + + + + Strvtion + + + + + IB: Akt-pT38 IB: Akt K-pT389 K IB: Rptor Gr1 shs6k1-a shs6k1-b shs6k1-c shrictor shrptor Gr1 c IB:

More information

Ulk λ PPase. 32 P-Ulk1 32 P-GST-TSC2. Ulk1 GST (TSC2) : Ha-Ulk1 : AMPK. WB: Ha (Ulk1) : Glu. h CON - Glu - A.A WB: LC3 AMPK-WT AMPK-DKO

Ulk λ PPase. 32 P-Ulk1 32 P-GST-TSC2. Ulk1 GST (TSC2) : Ha-Ulk1 : AMPK. WB: Ha (Ulk1) : Glu. h CON - Glu - A.A WB: LC3 AMPK-WT AMPK-DKO DOI: 10.1038/ncb2152 C.C + - + - : Glu b Ulk1 - - + λ PPse c AMPK + - + + : ATP P-GST-TSC2 WB: Flg (Ulk1) WB Ulk1 WB: H (Ulk1) GST (TSC2) C.C d e WT K46R - + - + : H-Ulk1 : AMPK - + - + + + AMPK H-Ulk1

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature10188 Supplementary Figure 1. Embryonic epicardial genes are down-regulated from midgestation stages and barely detectable post-natally. Real time qrt-pcr revealed a significant down-regulation

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nture09973 Plsm Memrne Phgosome TLR1/2/4 ROS Mitochondrion ROS OXPHOS Complex I ROS TRAF6 NADPH Oxidse Supplementry Figure 1 Model detiling the roles of mitochondril ROS in mcrophge cteril

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S1 - UTR m - 3HA - 2-1 hgh - 1 Uiquitin *! *! lk distl promoter m K3R/ K121R-3HA UTR hgh founder lines - HA - - founder lines TG- E1 L A2 B1 F9 G6 H4 H6 B C D2 G1 H3 J2 L - 7 IP: lk

More information

Supplementary Figure 1

Supplementary Figure 1 doi: 1.138/nture6188 SUPPLEMENTARY INFORMATION Supplementry Figure 1 c CFU-F colonies per 1 5 stroml cells 14 12 1 8 6 4 2 Mtrigel plug Neg. MCF7/Rs MDA-MB-231 * * MCF7/Rs-Lung MDA-MB-231-Lung MCF7/Rs-Kidney

More information

activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows

activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows Supplemental Data Supplemental Figure 1 compares CXCR4 expression in untreated CD8 + T cells, following activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows the

More information

Irs-2 coordinates Igf-1 receptor-mediated β-cell development and peripheral insulin signalling

Irs-2 coordinates Igf-1 receptor-mediated β-cell development and peripheral insulin signalling Irs-2 coordintes Igf-1 receptor-medited β-cell development nd peripherl insulin signlling Dominic J. Withers 1,2 *, Deorh J. Burks 1 *, Hether H. Towery 1, Shri L. Altmuro 1, Crrie L. Flint 1 & Morris

More information

Resident c-kit þ cells in the heart are not cardiac stem cells

Resident c-kit þ cells in the heart are not cardiac stem cells Reeived 3 Jul 05 Aepted Sep 05 Pulished 30 Ot 05 DOI: 0.038/nomms970 OPEN Resident -kit þ ells in the hert re not rdi stem ells Nisht Sultn, *, Lu Zhng, *, Jinyun Yn, *, Jiqiu Chen, Weiin Ci, Sheguft Rzzque,

More information

Moore-Morris et al. Supplemental Table 1.

Moore-Morris et al. Supplemental Table 1. Moore-Morris et al. Supplemental Table. In vivo echocardiographic assessment of cardiac size and function following transaortic constriction (T) at 7d and 8d. SHM 7d N=6 T 7d N=5 SHM 8d N= T 8d N=6 W,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI:.3/nc37 This Supplementry Informtion file ws updted with new reference (ref. 3) on Decemer WWW.NATURE.COM/NATURECELLBIOLOGY Mcmilln Pulishers Limited. All rights reserved logfc SUPPLEMENTARY INFORMATION

More information

Visual Cortex in Humans

Visual Cortex in Humans Visul Cortex in Humns 251 Visul Cortex in Humns B A Wndell, S O Dumoulin, nd A A Brewer, Stnford University, Stnford, CA, USA ã 2009 Elsevier Ltd. All rights reserved. Humn visul cortex comprises 4 6 illion

More information

Macmillan Publishers Limited. All rights reserved

Macmillan Publishers Limited. All rights reserved doi:1.138/nture127 Specilized filopodi direct long-rnge trnsport of SHH during verterte tissue ptterning Timothy A. Snders 1,2 *, Esther Llgoster 1,3,4 * & Mri Brn 1,3,4 The ility of signlling proteins

More information

Goal: Evaluate plant health effects while suppressing dollar spot and brown patch

Goal: Evaluate plant health effects while suppressing dollar spot and brown patch Newer Fungicide Products Alone nd In Rottion on Chicgo Golf Green Reserchers: Chicgo District Golf Assoc. Derek Settle, Tim Sibicky, nd Nick DeVries Gol: Evlute plnt helth effects while suppressing dollr

More information

Supplementary Figure 1. A microarray screen of organizers compared to non-organizer tissue reveals a putative organizer gene set.

Supplementary Figure 1. A microarray screen of organizers compared to non-organizer tissue reveals a putative organizer gene set. Supplementary Figure 1. A microarray screen of organizers compared to non-organizer tissue reveals a putative organizer gene set. (a, b) Venn diagrams of 31 enriched (a) and 17 depleted (b) genes significantly

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2697 Figure S1 Cytokeratin 5 is a specific marker for basal and intermediate cells in all mouse prostate lobes. (a) Immunofluorescence staining showing co-localization of YFP with p63 in

More information

* * * * * liver kidney ileum. Supplementary Fig.S1

* * * * * liver kidney ileum. Supplementary Fig.S1 Supplementry Fig.S1 liver kidney ileum Fig.S1. Orlly delivered Fexrmine is intestinlly-restricted Mice received vehicle or Fexrmine (100mg/kg) vi per os (PO) or intrperitonel (IP) injection for 5 dys (n=3/group).

More information

Feeding state and age dependent changes in melaninconcentrating hormone expression in the hypothalamus of broiler chickens

Feeding state and age dependent changes in melaninconcentrating hormone expression in the hypothalamus of broiler chickens Supplementry Mterils Epub: No 2017_23 Vol. 65, 2018 https://doi.org/10.183/bp.2017_23 Regulr pper Feeding stte nd ge dependent chnges in melninconcentrting hormone expression in the hypothlmus of broiler

More information

Single-Molecule Studies of Unlabelled Full-Length p53 Protein Binding to DNA

Single-Molecule Studies of Unlabelled Full-Length p53 Protein Binding to DNA Single-Molecule Studies of Unlbelled Full-Length p53 Protein Binding to DNA Philipp Nuttll, 1 Kidn Lee, 2 Pietro Ciccrell, 3 Mrco Crminti, 3 Giorgio Ferrri, 3 Ki- Bum Kim, 2 Tim Albrecht 1* 1 Imperil College

More information

Optimizing Metam Sodium Fumigation in Fine-Textured Soils

Optimizing Metam Sodium Fumigation in Fine-Textured Soils Optimizing Metm Sodium Fumigtion in Fine-Textured Soils Neil C Gudmestd University Distinguished Professor & Endowed Chir of Potto Pthology Deprtment of Plnt Pthology North Dkot Stte University Erly Dying

More information

Supplementary Figure 1

Supplementary Figure 1 Roles of endoplsmic reticulum stress-medited poptosis in -polrized mcrophges during mycocteril infections Supplementry informtion Yun-Ji Lim, Min-Hee Yi, Ji-Ae Choi, Jung-hwn Lee, Ji-Ye Hn, Sung-Hee Jo,

More information

Critical role of c-kit in beta cell function: increased insulin secretion and protection against diabetes in a mouse model

Critical role of c-kit in beta cell function: increased insulin secretion and protection against diabetes in a mouse model Dietologi (1) 55:14 5 DOI 1.17/s15-1-5-5 ARTICLE Criticl role of c-kit in et cell function: incresed insulin secretion nd protection ginst dietes in mouse model Z. C. Feng & J. Li & B. A. Turco & M. Riopel

More information

Chapter 5: The peripheral nervous system Learning activity suggested answers

Chapter 5: The peripheral nervous system Learning activity suggested answers Chpter 5: The peripherl nervous system Lerning ctivity suggested nswers Lerning Activity 5.1 (p. 222) 1 Briefly descrie the two min functions of the somtic nervous system. Description should refer to:

More information

Supplementary Information Titles

Supplementary Information Titles Supplementry Informtion Titles Journl: Nture Medicine Article Title: Corresponding Author: Modelling colorectl cncer using CRISPR-Cs9-medited engineering of humn intestinl orgnoids Toshiro Sto Supplementry

More information

Study of Stress Distribution in the Tibia During Stance Phase Running Using the Finite Element Method

Study of Stress Distribution in the Tibia During Stance Phase Running Using the Finite Element Method Ksetsrt J. (Nt. Sci.) 48 : 729-739 (2014) Study of Stress Distriution in the Tii During Stnce Phse Running Using the Finite Element Method Thepwchr Ruchirh 1, Tumrong Puttpitukporn 1, * nd Siriporn Ssimontonkul

More information

mir-124 acts through CoREST to control onset of Sema3A sensitivity in navigating retinal growth cones

mir-124 acts through CoREST to control onset of Sema3A sensitivity in navigating retinal growth cones cts through CoREST to control onset of Sem3A sensitivity in nvigting retinl growth cones Mrie-Lure Budet 1, Krishn H Zivrj 1, Cei Areu-Goodger 2, Alistir Muldl 1, Jvier Armisen 3, Cherie Blenkiron 3, Leonrd

More information

TLR7 induces anergy in human CD4 + T cells

TLR7 induces anergy in human CD4 + T cells TLR7 induces nergy in humn CD T cells Mrgrit Dominguez-Villr 1, Anne-Sophie Gutron 1, Mrine de Mrcken 1, Mrl J Keller & Dvid A Hfler 1 The recognition of microil ptterns y Toll-like receptors (TLRs) is

More information

SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28

SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28 Supp. Fig. 1 a APC b APC ICOS ICOS TCR CD28 mir P TCR CD28 P T cell Tolerance Roquin WT SG Icos mrna T cell Autoimmunity Roquin M199R SG Icos mrna www.nature.com/nature 1 Supp. Fig. 2 CD4 + CD44 low CD4

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S Tissue weights (g).... Liver Hert Brin Pncres Len mss (g) 8 6 -% +% 8 6 Len mss Len mss (g) (% ody weight) Len mss (% ody weight) c Tiilis nterior weight (g).6...... Qudriceps weight

More information

Supplementary Figures

Supplementary Figures Supplementary Figures Supplementary Figure 1. Confirmation of Dnmt1 conditional knockout out mice. a, Representative images of sorted stem (Lin - CD49f high CD24 + ), luminal (Lin - CD49f low CD24 + )

More information

Type II monocytes modulate T cell-mediated central nervous system autoimmunity

Type II monocytes modulate T cell-mediated central nervous system autoimmunity Type II monocytes modulte T cell-medited centrl nervous system utoimmunity Mrtin S. Weer, Thoms Prod homme, Swsn Youssef, Shnnon E. Dunn, Cynthi D. Rundle, Lind Lee, Jun C. Ptrroyo, Olf Stüve, Rymond A.

More information

Provider How To. Software Process Service Results

Provider How To. Software Process Service Results Softwre Proess Servie Results Provier How To Copyright Glenwoo Systems LLC 2010. The informtion herein remins the property of Glenwoo Systems LLC. This informtion my not e reprinte or uplite, n is governe

More information

Using Paclobutrazol to Suppress Inflorescence Height of Potted Phalaenopsis Orchids

Using Paclobutrazol to Suppress Inflorescence Height of Potted Phalaenopsis Orchids Using Pcloutrzol to Suppress Inflorescence Height of Potted Phlenopsis Orchids A REPORT SUBMITTED TO FINE AMERICAS Linsey Newton nd Erik Runkle Deprtment of Horticulture Spring 28 Using Pcloutrzol to Suppress

More information

FoxP3 + regulatory CD4 T cells control the generation of functional CD8 memory

FoxP3 + regulatory CD4 T cells control the generation of functional CD8 memory Received Fe Accepted 6 Jul Pulished 7 Aug DOI:.8/ncomms99 FoxP + regultory CD T cells control the genertion of functionl memory M.G. de Goër de Herve,,, S. Jfour,,, M. Vllée, & Y. Toufik, During the primry

More information

Connexin 30 sets synaptic strength. by controlling astroglial synapse invasion

Connexin 30 sets synaptic strength. by controlling astroglial synapse invasion Connexin 3 sets synptic strength y controlling stroglil synpse invsion Ulrike Pnnsch, Dominik Freche, Glenn Dllérc, Grégory Ghézli,, Crole Escrtin, Pscl Ezn, Mrtine Cohen-Slmon, Krim Benchenne, Veronic

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION 1. Supplementary Figures and Legends Supplementary Fig. 1. S1P-mediated transcriptional regulation of integrins expressed in OP/monocytoid cells. Real-time quantitative PCR analyses of mrna for two integrins,

More information

Author's personal copy

Author's personal copy Priming 1055 Priming R Henson, MRC Cognition nd Brin Sciences Unit, Cmridge, UK ã 2009 Elsevier Ltd. All rights reserved. Introduction Priming refers to chnge in ehviorl response to stimulus, following

More information

ARTICLE. E. Pavlova 1, N. Atanassova 1, C. McKinnell 2, R.M. Sharpe 2 1 Institute of Experimental Morphology, Pathology and Anthropology with Museum,

ARTICLE. E. Pavlova 1, N. Atanassova 1, C. McKinnell 2, R.M. Sharpe 2 1 Institute of Experimental Morphology, Pathology and Anthropology with Museum, DOI:.554/5YRTIMB..3 OPPOSITE MODELS OF EXPRESSION OF ANDROGEN RECEPTOR (AR) AND RETINOIC ACID RECEPTOR-α (RAR-α) IN THE ONSET OF MALE GERM CELL DEVELOPMENT IN HORMONALLY MANIPULATED RATS E. Pvlov, N. Atnssov,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION 1 SUPPLEMENTARY INFORMATION Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy Guillermo Luxán, Jesús C. Casanova, Beatriz Martínez-Poveda, Belén Prados,

More information

PNEUMOVAX 23 is recommended by the CDC for all your appropriate adult patients at increased risk for pneumococcal disease 1,2 :

PNEUMOVAX 23 is recommended by the CDC for all your appropriate adult patients at increased risk for pneumococcal disease 1,2 : PNEUMOVAX 23 is recommended y the CDC for ll your pproprite dult ptients t incresed risk for pneumococcl disese 1,2 : Adults ged

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementry Figure 1 Ncor1 Expression 2. 1.5 1..5. Muscle Liver Lmi Ppropi Kindey Pncres Lung Testis Bone Mrrow Thymus Spleen Peripherl Lymph nods Smll Intestine Ncor1 Expression 1.5 1..5. DN DP SP CD8

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:0.08/nture078 RNse VifHA VifHA βctin 6 Cell lyste IP: ntiha MG VifHA VifHA β ctin 6 7 Cell lyste IP: ntiha Supplementry Figure. Effect of RNse nd MG tretment on the Vif interction., RNse tretment does

More information