Recombinant adenovirus carrying glial cell line2derived neurotrophic factor gene protect midbra in dopaminergic neurons in mice

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1 256 ( ) JOURNAL OF PEKIN G UNIVERSITY( HEAL TH SCIENCES) Vol. 35 No. 3 J une 2003 ( ) [ ] ; ;MPTP [ ] : (r GDNF) (DA) 2 2 (MPTP) :LacZ (Ad2LacZ) 24 h 72 h 10 d 30 d X2Gal ;r GDNF (Ad2r GDNF) 72 h MPTP DA 1 DA 3 42 (DOPAC) ( HVA) :Ad2LacZ 24 h 72 h 10 d 30 d LacZ Ad2r GDNF DA MPTP MPTP + Ad2LacZ DA DOPAC HVA DOPAC/ DA HVA/ DA :Ad2r GDNF DA MPTP [ ] R74215 [ ] A [ ] X(2003) Recombinant adenovirus carrying glial cell line2derived neurotrophic factor gene protect midbra in dopaminergic neurons in mice GAO Hong WAN G Jianjun ZHAN G Wei J IAN G Yuhui N IU Dongbin WAN G Xiaomin (Neuroscience Research Institute Peking University ; Department of Neurobiology Peking University School of Basic Medical Sciences Beijing China) KEY WORDS Parkinson disease ; Glial cell line2derived neurotrophic factor ; Recombinant adenovirus ; Dopamine ; MPTP SUMMARY Objective : To observe the protective effects of recombinant adenovirus expressing glial cell line2derived neurot rop hic factor ( Ad2r GDN F) on t he midbrain dopaminergic neurons against M P TP damage. Methods : Recombinant adenovirus carrying LacZ gene ( Ad2LacZ) was injected into t he murine st riat um using t he stereotaxic apparat us. The murine brains were perf used and dissected t hen stained with X2Gal subsequently at 24 hours 72 hours 10 days and 30 days after injection. In another experiment MPTP was injected subcutaneously into the mice 72 hours after the intrastriatal injection of Ad2r GDNF ( pfu ml - 1 ). The murine striatum was removed to evaluate the content of DA DOPAC and HVA by HPLC2ECD 1 week later. Results : X2Gal staining indicated the expression of LacZ gene 24 hours after injection of Ad2LacZ. Stronger expression was also detected at the end of 72 hours and 10 days but decreased 30 days later. In addition the LacZ gene expression was localized and detectable in bot h neurons and glial cells. There was no apparent cytotoxic effect induced by Ad2 LacZ alone. In MPTP2treated mice the content of dopamine decreased 80 % compared to that in the saline2treated ones. The DA content of MPTP + Ad2r GDNF group on injected side was significantly higher t han t hat of M P TP alone group and M P TP + Ad2LacZ group. Changes in t he content of DOPAC and HVA followed t he same pattern. The ratio of DOPAC/ DA and HVA/ DA on injected side also decreased significantly. However no significant change was observed on uninjected side. Conclu2 sion : The result s suggested t hat recombinant adenoviral vector expressing r GDN F can protect DA neu2 rons against MPTP injury. ( J Peking U niv [ Health Sci ] : ) : ( G ) ( ) Supported by the Major State Basic Research Development Program of People s Republic of China ( G ) and the National Natural Science Foundation of China ( ) Corresponding author edu. cn

2 257 ( Parkinson s disease PD) PBS ml (dopamine DA) l h 48 h PD 45 d 68 DA d : (pfu DA ml - 1 ) = pfu (plaque forming unit pfu) ( glial cell line2derived neurot rop hic fac2 tor GDNF) TGF2 pfu ml pfu ml - 1 DA 1. 3 [1 DA ] C57BL/ 6J ( DA 62 (62hydroxydopa2 ) g 4 : mine 62OHDA) 2 2 (12met hyl242 phenyl2l tetrohydropyridine MPTP) Ad2LacZ MPTP ; ( 4) Ad2r GDNF [2 ] PD MPTP Ad2r GDNF GDNF Ad2LacZ MPTP PBS 72 h MPTP 40 mg kg HPLC2ECD [3 4 ] GDNF DA 3 42 (3 GDNF dihydroxyphenylacetic acid DOPAC) DA (homovanillic acid HVA) GDNF PD 1. 4 X2Gal GDNF PBS/ % ( MPTP PD ) min Ad2 PBS/ 3 (1 3 2 r GDNF DA MPTP 10 min) 37 X2Gal 24 h %( ) DMEM % PBS 0. 5 ml A (0. 4 mmol L - 1 HClO 4 23 ml [5 2 ml ] l2 h 5 % ( ) l min 10 min r min - 1 DMEM 15 min 1/ 2 2 ml B (6. 4 g g DMEM g ED TA ml ) l ml DMEM l ECD 0. 8 V 40 min ml 0. 9 ml DMEM 0. 1 ml GDNF LacZ (1) ; (2) MPTP ; (3) 70 % 80 % 90 % 5 min ml) 10 min r min min MPa 12 ml min l

3 258 ( ) JOURNAL OF PEKIN G UNIVERSITY( HEAL TH SCIENCES) Vol. 35 No. 3 J une Ad2r GDNF DA MPTP 1. 7 STA TISTICA One Way ANOVA Newman2Keuls post hoc gx s gx 2030 min 24 h Ad2LacZ pfu ml - 1 Ad2LacZ 4 l 24 h X2Gal Ad2r GDNF LacZ (1A B) 72 h MPTP DA (1 C D) 10 d DOPAC HVA DA MPTP 40 mg kg - 1 (1E F) 30 d LacZ DA DOPAC HVA (1 G F) (1) DA DOPAC/ DA HVA/ DA HVA/ DA 4 (2) C57BL/ 6J MPTP 40 mg kg min 1 Ad r GDNF MPTP DA DOPAC HVA Table 1 The effect of recombinant adenovirus Ad r GDNF on the content of DA DOPAC and HVA Group n Uninjection side DA DOPAC HVA Injection side DA DOPAC HVA Control MPTP MPTP + Ad2LacZ MPTP + Ad2r GDNF amount of substance per g tissue ; 3 P < P < vs the injection side of MPTP + Ad2r GDNF group1 2 Ad r GDNF MPTP DOPAC/ DA HVA/ DA Table 2 The effect of recombinant adenovirus Ad r GDNF on the ratio of DOPAC/ DA and HVA/ DA Group n Uninjection side DOPAC/ DA HVA/ DA Injection side DOPAC/ DA HVA/ DA Control MPTP MPTP + Ad2LacZ # # MPTP + Ad2r GDNF P < P < vs the injection side of MPTP + Ad2r GDNF group ; # P < vs the uninjection side of MPTP + Ad2LacZ group1

4 259 A( 50) B ( 200) : 24 hours after injection ; C( 50) D ( 200) : 72 hours after injection ; E( 50) F( 200) : 10 days after injection ; G( 50) H( 200) : 30 days after injection. 1 LacZ Figure 1 Expression of LacZ in murine striatum Ad2r GDNF 3 DA DOPAC HVA DA C57BL/ 6J MPTP 72 h pfuml - 1 Ad2r GDNF 62OHDA MPTP MPTP 4 l Ad2r GDNF DA PD MPTP [6 ] C57BL/ 6J Ad2LacZ 1 Ad2r GDNF Heikkila [7 ] C57BL/ 6J DA MPTP 40 mg kg - 1 MPTP 12 d Ad2LacZ (81 18) % DA 80 % DOPAC HVA ( 23 5 ) % DA PD DA DOPAC HVA Ad2r GDNF C57BL/ 6J MPTP Ad2LacZ 100 % 2 Ad2r GDNF DOPAC/ DA HVA/ DA MPTP Ad2 (2025 g) LacZ Ad2r GDNF DA 78 % DA DOPAC HVA MPTP DA Ad2LacZ 89 % MPTP DOPAC/ DA HVA/ DA MPTP C57BL/ 6J DA DA Ad2r GDNF Ad2 PD PD (2530 g) DA MPTP LacZ DA

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