Dynacare Laboratories Test Changes

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1 CHROMIUM CHROMIUM CHROM CHROM Plasma/ Whole blood 3.0 ml 3.0 ml Royal Blue Top (EDTA) Tube Royal Blue Top (EDTA) Tube Separate plasma/serum within 45 minutes of collection, and transfer to a plastic transport tube. Submit original unopened tube.in the nocturnal total parenteral nutrition patient, the sample will be drawn fasting in the afternoon, before the nocturnal solution is started for the evening Prep Prep Submit original unopened tube Inductively-coupled plasma-mass spectrometry (ICP-MS) Inductively-coupled plasma-mass spectrometry (ICP-MS) Environmental exposure: µg/l Environmental exposure: µg/l Test used to monitor industrial exposure to chromium. This test is not intended to monitor chromium levels in diabetic patients or in patients with suspected chromium deficiencies. Test used to monitor industrial exposure to chromium. This test is not intended to monitor chromium levels in diabetic patients or in patients with suspected chromium deficiencies. Changed ructions Changed

2 ETHOSUXIMIDE ETHOSUXIMIDE ETHOSUX ETHOSUX Zarontin Zarontin 1.0 ml 1.0 ml Red Top Tube. Do NOT use SSTs. Red Top Tube. Do NOT use SSTs. Oral Peak is 2-4 hours after dose. Oral Trough is immediately prior to next dose. Oral Peak is 2-4 hours after dose. Oral Trough is immediately prior to next dose. Prep to a Plastic Transport Tube. Prep to a Plastic Transport Tube. Immunoassay Immunoassay µg/ml; Potentially toxic: >100 µg/ml Critical >200 ug/ml µg/ml; Potentially toxic: >100 µg/ml Changed

3 ETHYLENE GLYCOL ETHYLENE GLYCOL ETHY GLYC ETHY GLYC ml 2.0 ml Red Top Tube or Dark Green Tube. DO NOT use SST. Red Top Tube. DO NOT use SST. Prep Centrifuge* and transfer to a Plastic Transport Tube Prep Centrifuge* and transfer to a Plastic Transport Tube d d Gas Liquid Chromatography Gas Liquid Chromatography Negative; Toxic Level: 10 mg/dl Negative; Toxic Level: 10 mg/dl Ethylene glycol is the basic ingredient in automobile antifreeze. Ethylene glycol is the basic ingredient in automobile antifreeze. Changed

4 HIV PHENOSENSE COMPREHENSIVE HIV PHENO Prep 87903, x Plasma 5.0 ml 2 Lavender Top (EDTA) Tubes Centrifuge* within 2 hours of collection and immediately transfer to a Purple Screw Cap Tube. FREEZE IMMEDIATELY and maintain frozen until tested. Freeze Frozen Direct measurement of HIV drug susceptibility Descriptive Report One time per week Viral load of 1000 copies or more required for testing. Submit separate frozen samples when requesting multiple tests on the same specimen. HIV PHENOSENSE COMPREHENSIVE HIV PHENO Prep 87903, x Plasma 5.0 ml 2 Lavender Top (EDTA) Tubes Centrifuge* within 2 hours of collection and immediately transfer to a Purple Screw Cap Tube. FREEZE IMMEDIATELY and maintain frozen until tested. Freeze Frozen Direct measurement of HIV drug susceptibility Descriptive Report One time per week Viral load of 1000 copies or more required for testing. Submit separate frozen samples when requesting multiple tests on the same specimen. Changed

5 MALARIA SMEAR MALARIA SMEAR MALAR SM MALAR SM Whole Blood Whole Blood 4.0 ml 4.0 ml Lavender Top (EDTA) Tube Lavender Top (EDTA) Tube should be collected at times of chill or fever. Invert immediately and mix with anticoagulant 8-10 times. In cases with travel history suggestive of malarial exposure, contact Dynacare laboratories to arrange for a STAT courier. s should be received in laboratory without delay to minimize potential false-negative results due to cell lysis. should be collected at times of chill or fever. Invert immediately and mix with anticoagulant 8-10 times. Prep Do NOT centrifuge. In cases with travel history suggestive of malarial exposure, contact Dynacare laboratories to arrange for a STAT courier. s should be received in laboratory without delay to minimize potential false-negative results due to cell lysis. Prep Do NOT centrifuge., preferred or, preferred or Manual, Microscopic examination of Wright's/Giemsa Stained Smears. Manual, Microscopic examination of Wright's/Giemsa Stained Smears. No Malarial Parasites seen No Malarial Parasites seen Smears positive for Malarial Parasites are reviewed by a Pathologist for speciation. Smears positive for Malarial Parasites are reviewed by a Pathologist for speciation. ructions Changed Prep Changed

6 OPIATES Qualitative, Urine U OPIATES Prep Codeine, Dihydrocodeine, Ethyl morphine, Heroin (diacetylmorphine), Hydrocodone, Hydromorphone, 6-monoacetylmorphine (6- MAM), Morphine-3-glucuronide,Morphine, Thebaine Heroin Urine (Random) 5.0 ml Plastic Urine Immunoassay No reference ranges are defined for drugs of abuse testing. Expected value: Negative Results <300 ng/ml are reported out as Negative OPIATES Qualitative, Urine U OPIATES Prep Codeine, Dihydrocodeine, Ethyl morphine, Heroin (diacetylmorphine), Hydrocodone, Hydromorphone, 6-monoacetylmorphine (6- MAM), Morphine-3-glucuronide, Thebaine Heroin Urine (Random) 5.0 ml Plastic Urine Immunoassay No reference ranges are defined for drugs of abuse testing. Expected value: Negative Results <300 ng/ml are reported out as Negative Results 300 ng/ml are reported out as Positive Results 300 ng/ml are reported out as Positive This is only a screening test. Positive results should be confirmed by an alternate method. This test does not detect oxycodone and/or oxymorphone. For oxycodone and/or oxymorphone screening, order Urine Oxycodone Screen (U OXY SCR, ). This is only a screening test. Positive results should be confirmed by an alternate method. This test does not detect oxycodone and/or oxymorphone. For oxycodone and/or oxymorphone screening, order Urine Oxycodone Screen (U OXY SCR, ). PDM Changed

7 SEMEN EXAMINATION POST-VASECTOMY POSTVASEC Prep Semen Entire Ejaculate Plastic Screw Cap Abstain from sexual activity for at least 3 days before the specimen is collected. must be maintained at body temperature and submitted to the lab within 4 hours of collection. Post vasectomy must be noted on the Laboratory Request form. Completed specimen information form and specimen may be submitted between the hours of 7:00 AM - 9:00 PM. For specimen information forms, please call and specimen information form must be submitted to the lab within 4 hours of collection and maintained at body temperature. Maintain at body temperature (37 C) Body Temperature Microscopic examination of semen Reported as presence or absence of sperm Motility will be checked, if sperm present. SEMEN EXAMINATION POST-VASECTOMY POSTVASEC Prep Semen Entire Ejaculate Plastic Screw Cap Abstain from sexual activity for at least 3 days before the specimen is collected. must be maintained at body temperature and submitted to the lab within 4 hours of collection. Post vasectomy must be noted on the Laboratory Request form. Completed specimen information form and specimen may be submitted between the hours of 7:00 AM - 9:00 PM. For specimen information forms, please call must be submitted to the lab within 4 hours of collection and maintained at body temperature. Maintain at body temperature (37 C) Body Temperature Microscopic examination of semen Reported as presence or absence of sperm Motility will be checked, if sperm present. Ejaculates are centrifuged to improve the detection of sperm. Prep Changed Changed

8 VITAMIN D 25 HYDROXY VITD25OH Prep Vitamin D 25 Hydroxy (Cholecalciferol), Vitamin D3 1.5 ml SST or Red Top Tube to Plastic Transport Tube. Starting only serum will be accepted. Direct Competitive Chemiluminescence Immunoassay (CLIA) ng/ml; Recent studies consider the lower limit of 32.0 ng/ml to be a threshold for optimal health. Values of vitamin D vary with exposure to sunlight. There are also variations during the menstrual cycle, particularly at the time of ovulation. concentrations of 25-D are also known to vary with age, sex, race, season, and geographic location. VITAMIN D 25 HYDROXY VITD25OH Prep Vitamin D 25 Hydroxy (Cholecalciferol) 1.5 ml SST or Red Top Tube to Plastic Transport Tube. Starting only serum will be accepted. Direct Competitive Chemiluminescence Immunoassay (CLIA) ng/ml; Recent studies consider the lower limit of 32.0 ng/ml to be a threshold for optimal health. Values of vitamin D vary with exposure to sunlight. There are also variations during the menstrual cycle, particularly at the time of ovulation. concentrations of 25-D are also known to vary with age, sex, race, season, and geographic location. Changed

9 VOLATILES SCREEN VOLATILE Prep Methanol, Alcohol/ETOH, Acetone, Isopropyl 2.0 ml Red Top Tube or Dark Green Tube Do not use alcohol to cleanse arm to Plastic Transport Tube d Gas Liquid Chromatography Negative VOLATILES SCREEN Prep VOLATILE Methanol, Alcohol/ETOH, Acetone, Isopropyl 2.0 ml SST or Red Top Tube Do not use alcohol to cleanse arm to Plastic Transport Tube d Gas Liquid Chromatography Negative Toxic levels: Ethanol: >0.300 g/dl; Acetone: >50 mg/dl; Isopropanol: >10 mg/dl; Methanol: >20 mg/dl Toxic levels: Ethanol: >0.300 g/dl; Acetone: >50 mg/dl; Isopropanol: >10 mg/dl; Methanol: >20 mg/dl Changed

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