Supplementary table I. Real-time primers used in the study. The fold change was obtained by
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1 Supplementary table I. Real-time primers used in the study. The fold change was obtained by normalizing the gene expression number to those of HPRT, then comparing the samples to untreated or naive mice.
2 Supplementary table II. Colocalization analysis for images in figure 6, Pearson s correlation coefficient and % of MMP13 colocalized with F4/80, SMA and Gr1.
3 Supplementary figure S1. C57BL/6 (WT) and mmp12 -/- mice were infected with approximately 30 infective cercariae of S. mansoni and then euthanized on wk 9. Liver tissues were fixed in Bouin-Hollande solution and stained with Giemsa. Number of Eosinophils (a) and mast cells (b) were counted in liver granulomas. Hydroxyproline levels normalized to worm pairs (c) are shown. Statistical significance is measured using unpaired student s t test (n=14/group). The data are combined from two independent experiments that produced similar results.
4 Supplementary figure S2. C57BL/6 (WT) and mmp12 -/- mice were infected with 30 infective cercariae of S. mansoni and euthanized on wk 12. Liver tissues were fixed in Bouin-Hollande solution and stained with Giemsa. Average granuloma volumes and liver hydroxyproline levels normalized to egg count are shown. Statistical significance is measured using unpaired student s t test (n=14/group). The data are combined results from two independent experiments that produced similar results. Supplementary figure S3. Col 1a, Col 3a and Col 6a expression in liver tissues at 9 wk infection with S. mansoni. Values were normalized to hypoxanthine phosphoribosyltransferase (hprt), and fold changes in WT and mmp12 -/- were generated by comparing with WT and mmp12 -/- unchallenged mice, respectively. The data show the means ± SEM. Statistical significance of data was determined using one-way ANNOVA (n=5/group)
5 Supplementary figure S4. C57BL/6 (WT) and mmp12 -/- mice were infected with approximately 30 infective cercariae of S. mansoni and euthanized after 9 weeks. Liver tissues were fixed in Bouin-Hollande solution and stained with Giemsa. Numbers of eosinophils were counted in liver granulomas. The data show the means ± SEM.
6 Supplementary figure S5. WT and mmp12 -/- mice were sensitized and challenged with S. mansoni eggs. On day 8 post-challenge, bronchoalveolar lavage (BAL) fluid was recovered. Numbers of cells in the BAL fluid were plotted as means ± SEM. BAL fluids were analyzed for IL-13 using a multipex cytokine analysis method. The data show the means ± SEM. One of two independent experiments are shown. Supplementary figure S6. WT and mmp12 -/- mice were sensitized and challenged with S. mansoni eggs. On day 8 post-challenge, real-time PCR analysis was performed on 50 mg lung samples obtained from WT and mmp12 -/- mice. Genes analyzed: tgf-β and egr-1 as described in methods.
7 Supplementary figure S7. WT and mmp12 -/- mice were sensitized and challenged with S. mansoni eggs. On day 8 post-challenge, real-time PCR analysis was performed on 50 mg lung samples obtained from WT and mmp12 -/- mice. Genes analyzed include col 1a, Col 3a and col 6a as described in methods. Supplementary figure S8. BMDM were seeded at a concentration of per well in 24- well plates and stimulated with IL-4, IL-13, IL-21, IFN-γ and TNF-α at a concentration of 20 ng/ml for 16 h. Real-time PCR analysis was performed for mmp12 and mmp13 and values were normalized to hprt and compared to untreated cells. The data show the means ± SEM.
8 Supplementary figure S9. Thioglycollate-elicited mouse peritoneal macrophages were seeded at a concentration of per well in 24-well plates and stimulated with IL-4 (20 ng/ml) in the presence and absence of anti-il4rα, anti-il2γc, or isotype control Ab for a period of 16 h. Real-time PCR analysis was performed for mmp13 expression and values were normalized to hprt and compared to untreated cells. The data show the means ± SEM. Similar results were obtained in two independent experiments.
9 Supplementary figure S10. Thioglycollate-elicited mouse peritoneal macrophages were seeded at a concentration of per well in 24-well plates and stimulated with IL-13 (20 ng/ml) in the presence and absence of anti-il4rα, anti-il2γc, or isotype control Ab for a period of 16 h. Real-time PCR analysis was performed for mmp13 expression and values were normalized to hprt and compared to untreated cells. The data show the means ± SEM. Similar results were obtained in two independent experiments.
10 Supplementary figure S11. C57BL/6 (WT) and mmp12 -/- mice were infected with 30 infective cercariae of S. mansoni and euthanized after 9 weeks. Liver tissues were fixed in Bouin-Hollande solution and stained with Giemsa. Macrophages present at the center or periphery of the granuloma as well as the total number were enumerated. The data show the mean ± SEM. The data are combined results from two independent experiments that produced similar results.
11 Supplementary figure S12. S. mansoni egg challenged lungs from wild type and mmp12 -/- mice were fixed in Bouin-Hollande solution and stained with Giemsa. Macrophages present at the center or periphery of the granuloma as well as the total number were enumerated. The data show the means ± SEM. The data are combined results from two independent experiments that produced similar results.
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<10. IL-1β IL-6 TNF + _ TGF-β + IL-23
3 ns 25 ns 2 IL-17 (pg/ml) 15 1 ns ns 5 IL-1β IL-6 TNF
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a. b. c. d. e. f. g. h. i. j. k. l. 2.5 2 1.5 1.5 IL-1β 12 8 6 4 2 IL-1β 9 8 7 6 4 3 3 2.9 IL-1β m. n. o. p. 1.8 1.6 1.4 1.2 1.8.6.4.2 6h LPS 2 15 1 5 6h LPS 2 6h LPS 6 4 3 6h LPS Supplementary Figure
Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols
Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols used to differentiate mouse ESCs. (b) Representative
Supplementary Figure 1
d f a IL7 b IL GATA RORγt h HDM IL IL7 PBS Ilra R7 PBS HDM Ilra R7 HDM Foxp Foxp Ilra R7 HDM HDM Ilra R7 HDM. 9..79. CD + FOXP + T reg cell CD + FOXP T conv cell PBS Ilra R7 PBS HDM Ilra R7 HDM CD + FOXP
Supplementary Figure 1. H-PGDS deficiency does not affect GI tract functions and anaphylactic reaction. (a) Representative pictures of H&E-stained
1 2 3 4 5 6 7 8 9 10 11 Supplementary Figure 1. H-PGDS deficiency does not affect GI tract functions and anaphylactic reaction. (a) Representative pictures of H&E-stained jejunum sections ( 200 magnification;
ILC1 and ILC3 isolation and culture Following cell sorting, we confirmed that the recovered cells belonged to the ILC1, ILC2 and
Supplementary Methods and isolation and culture Following cell sorting, we confirmed that the recovered cells belonged to the, ILC2 and subsets. For this purpose we performed intracellular flow cytometry
Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β
Supplementary Figures Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β and LPS. Non-parenchymal liver cells were isolated and treated with or without TGF-β
Pair-fed % inkt cells 0.5. EtOH 0.0
MATERIALS AND METHODS Histopathological analysis Liver tissue was collected 9 h post-gavage, and the tissue samples were fixed in 1% formalin and paraffin-embedded following a standard procedure. The embedded
Nature Immunology: doi: /ni Supplementary Figure 1. Cellularity of leukocytes and their progenitors in naive wild-type and Spp1 / mice.
Supplementary Figure 1 Cellularity of leukocytes and their progenitors in naive wild-type and Spp1 / mice. (a, b) Gating strategies for differentiated cells including PMN (CD11b + Ly6G hi and CD11b + Ly6G
Supporting Information
Supporting Information Stegbauer et al. 10.1073/pnas.0903602106 SI Methods Analysis of Plasma Renin Activity (PRA) and ACE Activity. PRA and serum ACE activity levels were determined by RIA (RENCTK, DiaSorin;
Nature Immunology: doi: /ni Supplementary Figure 1
Supplementary Figure 1 Fatty acid oxidation is emphasized in 1 macrophages compared with that in macrophages. Gene expression of mitochondrial OXPHOS (Atp5j, Cox4i1, Uqcrc1/2, Ndufs1, Sdhb) and β-oxidation
Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD-
Supplementary Methods Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD- L1 (10F.9G2, rat IgG2b, k), and PD-L2 (3.2, mouse IgG1) have been described (24). Anti-CTLA-4 (clone
Supplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
Bile Acids and Liver Fibrosis Causative Agent and Therapeutic Tool
Bile Acids and Liver Fibrosis Causative Agent and Therapeutic Tool Peter Fickert, Andrea Fuchsbichler, Tarek Moustafa, Gernot Zollner, Emina Halilbasic, Ulrike Stöger, Cord Langner, Helmut Denk, and Michael
Supplementary Figures. T Cell Factor-1 initiates T helper 2 fate by inducing GATA-3 and repressing Interferon-γ
Supplementary Figures T Cell Factor-1 initiates T helper 2 fate by inducing GATA-3 and repressing Interferon-γ Qing Yu, Archna Sharma, Sun Young Oh, Hyung-Geun Moon, M. Zulfiquer Hossain, Theresa M. Salay,