TOUCAN DATA & SCIENCE INFORMATION

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Cathleen Dixon
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TOUCAN DATA & SCIENCE INFORMATION RESEARCH Centrego s Toucan ElectroChemical Activation (ECA) devices generate a powerful sanitizing and disinfecting solution containing hypochlorous acid, the same

Studies show that while hypochlorous acid is harmless to humans and animals, it is highly effective in killing all types of bacteria, viruses, and fungal spores.

Study by the Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang University (Korean FDA) The KFDA (Korean FDA) Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang

1 TOUCAN DATA & SCIENCE INFORMATION RESEARCH Centrego s Toucan ElectroChemical Activation (ECA) devices generate a powerful sanitizing and disinfecting solution containing hypochlorous acid, the same natural antimicrobial compound produced by the human body to fight pathogens. Studies show that while hypochlorous acid is harmless to humans and animals, it is highly effective in killing all types of bacteria, viruses, and fungal spores. The following studies scientifically document the extreme efficiency of the solutions you can generate with Toucan devices. 1. Study by the Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang University (Korean FDA) The KFDA (Korean FDA) Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang University, South Korea completed a study on the solutions produced by Toucan devices. The results of the study show: The electrolyzed water produced from the Toucan range has a strong disinfecting effect against general bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), vacomycin-resistant Enterococcus faecium (VRE), E. coli, Salmonella, Shigella, and it is proved that it can be useful as a sterilizing/disinfecting agent for hygiene control to prevent the occurrence of food poisoning and for control against infectious diseases. The Coach House, Newbury, Frome, Somerset BA11 3RG Page 1 of 7

2 Table 1. Biocidal activity of electrolyzed water produced by the Toucan against various microorganisms according to exposure time Test microorganism Initial count(cfu /ml) Colonies remaining (CFU/mL) 0.5 min 1 min 2 min 5 min 10 min methicillin-susceptible < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Staphylococcus aureus methicillin-resistant < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Staphylococcus aureus vancomycin-resistant < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Enterococcus faecium Enterococcus faecalis < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Escherichia coli < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Klebsiella pneumoniae < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Enterobacter cloacae < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Salmonella Typhi < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Salmonella Enteritidis < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Shigella sonnei < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Pseudomonas aeruginosa > 10 3 < 10 2 < 10 2 < 10 2 < 10 2 Acinetobacter baumannii < 10 2 < 10 2 < 10 2 <10 2 < 10 2 Stenotrophomonas maltophilia > 10 3 < 10 2 < 10 2 < 10 2 < 10 2 Staphylococcus aureus, ATCC < 10 2 < 10 2 < 10 2 < 10 2 < E. coli, ATCC < 10 2 < 10 2 < 10 2 < 10 2 < 10 2 Pseudomonas aeruginosa, ATCC > 10 2 < 10 2 < 10 2 < 10 2 < Bacillus subtilis, ATCC 6633(vegetative form) > 10 3 < 10 2 < 10 2 < 10 2 < Study by Prof T.E. Cloete, Department of Microbiology and Plant Pathology According to the paper MICROBIOLOGICAL FINGERPRINTING OF ANOLYTE by Professor T.E. Cloete, Department of Microbiology and Plant Pathology, Faculty of Natural, Agricultural and Information Sciences, the hydrochlorous acid (also called Anolyte) produced by Toucan devices is highly effective in killing a wide range of bacterial strains, even at 50 parts per million. According to the study: Anolyte gave a 100 % kill of all the test isolates at a concentration of 100 % and 10 % (Table 1). The Coach House, Newbury, Frome, Somerset BA11 3RG Page 2 of 7

3 Table 1: Percentage Kill of bacterial strains at different anolyte concentrations Concentrations of Anolyte Bacterial Strain Gram Stain 100% 1:10 1:20 Bacillus subtilis Pseudomonasaeruginosa Acinetobactercalcoaceticus Lactobacillusbrevis Micrococcusluteus Streptococcusfeacalis Pseudomonasflourescens Staphylococcusaureus Pseudomonasalcaligenes The Coach House, Newbury, Frome, Somerset BA11 3RG Page 3 of 7

4 Concentrations of Anolyte Bacterial Strain Gram Stain 100% 1:10 1:20 Pseudomonasmedocina Pseudomonasputida Bacilluscereus Micrococcusroseus Pseudomonasstutzeri Pseudomonassyringae Campylobacter jejuni Not determined 3. PA Department of Agriculture presentation on NoroVirus According to researchers at Pennsylvania Department of Agriculture, foodborne illness caused by NoroVirus (NoV) is a major health problem, and just as widespread as the betterknown Salmonella. According to PA Department of Agriculture, the hypochlorous acid solution produced by Toucan devices effectively kills NoV with an effectiveness far exceeding that of bleach (sodium hypochlorate), UV light, high hydrostatic pressure or heat. The Coach House, Newbury, Frome, Somerset BA11 3RG Page 4 of 7

5 SCIENCE The technology behind the Toucan systems is based on electrochemical activation of water, and has been developed in line with the world trend to reduce the amount of chlorine and other chemicals currently used to purify, disinfect, and sterilize. The Toucan devices create a non-toxic, non-irritant, environmentally and ecologically safe sanitizing solution (hypochlorous acid or Anolyte) produced from the electrochemical reaction of water, salt and electricity. Hypochlorous acid is proven to be more effective against bacteria, viruses, noroviruses, molds and other microbiological organisms than Chlorine solutions alone and, yet, remains food-safe and non-harmful to humans, pets, and most fabrics/surfaces. Studies have shown that application of Anolyte (hypochlorous acid) results in substantial reduction or simple eradication of the following (non-inclusive) list of examples: Bacillus anthraces (Anthrax)Bacillus cereus Bacillus stearothermophilus Bacillus subtilis spore Bacteriophage (F2) Clostricium perfringens spore Coliohage MS2 Cryptosporidium parvum oocyst Escherichia Coli (e-coli) Francisella tularensis Giardia muris cyst Leprosy All Avian Flu s (H1N1-H1N5) Hepatitis A Virus (HAV)Hepatitis virus analog (F2) Klebsiella terrigena Legionella pneumopnila Listeria monocytogenes (Listeria) Psedumonas aeruginosa Salmonella typhimurium Staphylococcus aureus Vaccina virus (smallpox) Vibrio cholerae (Cholera) MRSA Mold Plaque Many more The Coach House, Newbury, Frome, Somerset BA11 3RG Page 5 of 7

6 OPERATION With the Toucan systems, creating powerful sanitizing and disinfecting solution is as easy as Fill the Toucan handheld spray unit or jug with ordinary tap water. 2. Add a scoop of ordinary table salt or chemical-free, food-grade catalyst, and swirl lightly. 3. Place the dispenser on the base station/charger and press Start. A blue light will pulsate for approximately 5 minutes while the solution is being activated. When the blue light stops pulsating, your powerful, chemical-free Toucan solution is ready to sanitize and disinfect with times more power than chlorine. The Toucan sanitizing/disinfecting solution kills the following microbes in one minute or less. GERM KILL % RATIO E-Coli Listeria MRSA Noro Virus H1N1 / Flu Salmonella Staph / VRE Pseudomonas The Coach House, Newbury, Frome, Somerset BA11 3RG Page 6 of 7

7 OVERVIEW The Toucan: A Breakthrough Innovation in the War on Germs Until now, keeping one s household or workplace free from harmful bacteria and diseasecausing microorganisms often meant maintaining cabinets full of expensive, environmentally unfriendly chemical disinfectants and sanitizers. Centrego Ltd, a leader in green cleaning and sanitizing systems, has changed all that with the release of an innovative new weapon in the fight against household germs: the Toucans. The Toucan systems consist of a compact docking station and hand-held applicator that together create a powerful, chemical-free sanitizing/disinfecting solution that is times more powerful than chlorine, yet is non-toxic and non-hazardous. Using the convenient and economical Toucan systems, consumers can now kill infectioncausing bacteria, viruses, fungi and spores without the expense and health hazards associated with chemically based cleaning products. For more information on the Toucan system or the science please contact us on the below telephone no. The Coach House, Newbury, Frome, Somerset BA11 3RG Page 7 of 7

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