SUMMARY OF ANTIMICROBIAL ACTIVITY LOCK & FILL #3
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1 SUMMARY OF ANTIMICROBIAL ACTIVITY LOCK & FILL #3 Neutral Disinfectant & Detergent Description Lock & Fill #3 Neutral Disinfectant & Detergent is a broad spectrum, neutral ph, hard surface disinfectant. When used as directed, this product will deliver effective biocidal action against bacteria, fungi, and viruses. This formulation is a blend of a premium active ingredients and inerts: surfactants, chelates, and water. Biocidal performance is attained when this product is properly diluted at ½ oz. per gallon or 1:256. Lock & Fill #3 can be used to disinfect a wide variety of hard surfaces such as floors, walls, and countertops in hospitals, households, and institutions. Regulatory Summary Physical Properties EPA Registration No ph of Concentrate Flash Point (PMCC) >200 F 8325 USDA Authorization None Specific 25 C 0.99 % Quat (mol. wt.360.5) California Status None Pounds per 8.34 % Volatile C Canadian PCP# None Canadian Din # None (rev. 11/07/05 jcg)
2 Summary of Antimicrobial Test Results Lock & Fill #3 Neutral Disinfectant & Detergent is a "One-Step" Hospital Disinfectant, Virucide, Fungicide, Mildewstat, and Cleaner. Listed below, and in the following pages, is a summary of the Antimicrobial Claims and a review of the Antimicrobial Test Results. Claim: Contact Time: Organic Soil: Water Conditions: Disinfectant 10 minutes 5% 400 ppm as CaCO 3 Test Method: Official Method of the AOAC, 14 Edition Use Dilution Method Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Pseudomonas ppm 400 ppm 60, 60, 60 0/60, 0/60, 0/60 aeruginosa (0.50 oz/gal) Staphylococcus ppm 400 ppm 60, 60, 60 0/60, 0/60, 0/60 aureus Salmonella ppm 400 ppm 60, 60, 60 0/60, 0/60, 0/60 choleraesuis Acinetobacter ppm 400 ppm 10, 10 0/10, 0/10 calcoaceticus Bordetella ppm 400 ppm 10, 10 0/10, 0/10 bronchiseptica Chlamydia VR ppm 400 ppm 10, 10 0/10, 0/10 psittaci Enterobacter ppm 400 ppm 10, 10 0/10, 0/10 aerogenes Enterobacter ppm 400 ppm 10, 10 0/10, 0/10 cloacae Enterococcus ppm 400 ppm 10, 10 0/10, 0/10 (Streptococcus) faecalis (Vancomycin Resistant) (VRE) Escherichia coli ppm 400 ppm 10, 10 0/10, 0/10 Escherichia coli 1 (Clinical Isolate) 660 ppm 400 ppm 10, 10 0/10, 0/10 (Clinical Isolate) Fusobacterium ppm 400 ppm 10, 10 0/10, 0/10 necrophorum Klebsiella ppm 400 ppm 10, 10 0/10, 0/10 pneumoniae 2 Legionella ppm 400 ppm 10, 10 0/10, 0/10 pneumophila Listeria monocytogenes ppm 400 ppm 10, 10 0/10, 0/10 1Resistant to the Antibiotics: Ampicillin, Carbenicillin, Kanamycin, and Tetracycline. 2Resistant to the Antibiotics: Ampicillin, Carbenicillin, Chloramphenicol, and Tetracycline.
3 Summary of Antimicrobial Test Results - (Continued) Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Pasteurella ppm 400 ppm 10, 10 0/10, 0/10 multocida Proteus mirabilis ppm 400 ppm 10, 10 0/10, 0/10 Proteus vulgaris ppm 400 ppm 10, 10 0/10, 0/10 Pseudomonas Clinical Isolate 660 ppm 400 ppm 10, 10 0/10, 0/10 aeruginosa 3 Salmonella ppm 400 ppm 10, 10 0/10, 0/10 enteritidis Salmonella ppm 400 ppm 10, 10 0/10, 0/10 Typhi Salmonella ppm 400 ppm 10, 10 0/10, 0/10 Typhimurium Serratia ppm 400 ppm 10, 10 0/10, 0/10 marcescens Shigella ppm 400 ppm 10, 10 0/10, 0/10 Flexneri Shigella ppm 400 ppm 10, 10 0/10, 0/10 Sonnei Staphylococcus ppm 400 ppm 10, 10 0/10, 0/10 aureus 4 (MRSA) Staphylococcus Clinical Isolate 660 ppm 400 ppm 10, 10 0/10, 0/10 aureus 5 Staphylococcus CDC No. HIP- 660 ppm 400 ppm 10, 10 0/10, 0/10 aureus 6 (VISA) 5836 Staphylococcus Clinical Isolate 660 ppm 400 ppm 10, 10 0/10, 0/10 epidermidis 7 Streptococcus ppm 400 ppm 10, 10 0/10, 0/10 (Enterococcus) faecalis 8 Streptococcus ppm 400 ppm 10, 10 0/10, 0/10 (Enterococcus) faecalis Streptococcus pyogenes ppm 400 ppm 10, 10 0/10, 0/10 Conclusion: All lots of Lock & Fill #3 effectively killed the above listed bacteria as specified in the test performance standards. Lock & Fill #3 meets EPA requirements for hard surface disinfectant claims in hospital and medical environments when diluted to 660 ppm active concentration in 400 ppm synthetic hard water, and in the presence of 5% organic soil. 3Resistant to the Antibiotics: Amikacin, Ampicillin, Carbenicillin, Cefamandole, Cefazolin,Cefoxitin, Chloramphenicol, Kanamycin, and Tetracycline. 4 Resistant to Gentamicin and Methicillin 5 Resistant to the Antibiotics: Cefazolin, Clindamycin, Erythomycin, Gentamicin, Kanamycin, Methicillin, Penicillin, Tetracycline and Tobramycin 6 Reduced Susceptibility to Vancomycin 7 Resistant to the Antibiotics: Cefazolin, Chloramphenicol, Clindamycin, Erythomycin, Gentamicin, Kanamycin, Methicillin, Penicillin, Tetracycline and Tobramycin 8 Resistant to the Antibiotics: Cefazolin, Chloramphenicol, Clindamycin, Erythomycin, Gentamicin, Kanamycin, Methicillin, Penicillin, Tetracycline and Tobramycin
4 Summary of Antimicrobial Test Results - (Continued) Claim: Contact Time: Organic Soil: Water Conditions: Mildewstat 10 minutes 5% 400 ppm as CaCO 3 Test Method: Mildewstat (Mold and Mildew Control) - EPA - TSD Mildewstat on Hard Surfaces Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Aspergillus niger ppm (0.50 oz/gal) 400 ppm 10, 10 0/10, 0/10 Conclusion: All lots of Lock & Fill #3 were effective against Aspergillus niger under the test conditions outlined in the EPA test performance standards described above. Lock & Fill #3 is an effective mildewstat for non-porous inanimate hard surfaces when diluted to 660 ppm active concentration in 400 ppm synthetic hard water and in the presence of 5% organic soil. Claim: Contact Time: Organic Soil: Water Conditions: Fungicide 10 minutes 5% 400 ppm as CaCO 3 Test Method: Official Method of Analysis of the AOAC Fungicidal Test. Organism ATCC# Dilution Replicate Results s 5 Min 10 Min 15 Min Trichophyton ppm (0.50 oz/gal) 4 0/ mentagrophytes Candida albicans ppm (0.50 oz/gal) 4 0/ Conclusion: All lots of Lock & Fill #3 effectively killed Trichophyton mentagrophytes and Candida albicans as specified in the test performance standards. Lock & Fill #3 is an effective fungicide for non-porous inanimate hard surfaces when diluted to 660 ppm active concentration in 400 ppm synthetic hard water and in the presence of 5% organic soil.
5 Summary of Antimicrobial Test Results - (Continued) Claim: Contact Time: Organic Soil: Water Conditions: Virucide Varies 5% 400 ppm as CaCO 3 Test Method: EPA Guidelines Organism Adenovirus Type 4 Source of Virus or ATCC# ATCC VR-4 strain RI-67 Host System; Cytopathic Effect H. Ep. #2 cells Cytopathic Effects Use-Dilution Concentratio n 660 ppm (0.50 oz/gal) Contact Time 10 Min. 5.5, 5.5 Reduction (Log 10) of Virus Titer Herpes Simplex Type 1 Herpes Simplex Type 2 HIV-1 (AIDS Virus) Influenza A/ Hong Kong Hepatitis B HSV-1; ATCC VR- 733 HSV-2; MS Strain HTLV-IIIRF; NCI ATCC 68-H3N2 Hepadnavirus Testing, Inc. (DHBV) Bovine Viral Diarrhea Virus VR-740 Strain 229E VERO cells; lytic cytopathic effect VERO cells; lytic cytopathic effect MT2 cells; lytic cytopathic effect MDCK cells; lytic cytopathic effect Primary Duck Hepaocytes No Cytopathic Effects 660 ppm 10 Min. >7.5, ppm 10 Min. >6.5, ppm 4 Min. >3.0, ppm 10 Min. >8..0, ppm (1/2 oz./gal) 10 min. 4.8, 4.8 Hepatitis C (HCV) MDBK Cells 660 ppm (1/2 oz./gal) 10 min. 5.5, 5.7 Human Corona MRC-5 Host 660ppm 10 Min. >4.0, >4.0 Virus Rubella virus Strain M-33 RK13 cells; cytopathic 660 ppm 10 Min. >5.0, 5.0 effect Rabies virus ATCC VR ppm 10 Min. 4.5, 4.5 Respiratory ATCC VR ppm 10 Min. 4.0, 4.5 Syncytial virus Vaccinia Strian IHD VERO 660 ppm 10 Min. >7..0, 7.0 Cells; lytic cytopathic effect Avian Infectious ATCC VR ppm 10 Min. 6.0, 6.25 Bronchitis virus Avian lab isolate 660 ppm 10 Min 4.0, 6.0 Polyomavirus Canine Parvovirus Canine Distemper virus Feline Leukemia Virus Feline Picornavirus Infectious Bovine Rhinotracheitis Pseudorabies Virus Transmissible Gastroenteritis Type 2 (CPV) NFLK Cells 2640 ppm 2 oz./gal. 10 Min. >4.0, >4.0 ATCC-VR ppm 10 Min. 3.5, 3.5 ATCC VR ppm 10 Min. 4.5, 4.75 Strain FL-237 ATCC VR ppm 10 Min. 5.0, 5.0 ATCC VR ppm 10 Min 8.0,8.0 ATCC VR ppm 10 Min. 5.5, 5.5 ATCC VR ppm 10 Min. 3.5, 3.5
6 Conclusion: All lots of Lock & Fill #3 effectively inactivated the above listed viruses as specified in the test performance standards. Lock & Fill #3 meets EPA requirements for hard surface virucidal claims in hospital and medical environments when diluted to 660 ppm in 400 ppm A.O.A.C. synthetic hard water and in the presence of 5% organic soil.
7 Summary of Antimicrobial Efficacy - Etiology 9 Pathogenic Microorganism Pseudomonas aeruginosa Staphylococcus aureus Salmonella choleraesuis Acinetobacter calcoaceticus Aspergillus niger Bordetella bronchiseptica Candida albicans Chlamydia psittaci Enterobacter aerogenes Enterobacter cloacae Fusobacterium necrophorum Description Gram negative bacteria identified as a major cause of hospital acquired (nosocomial) infections. Causes wound infections (especially burn), meningitis, pneumonia and eye infections. Required for Hospital Disinfectants. Gram positive bacteria identified as a major cause of hospital acquired (nosocomial) infections. Colonizes food and secretes enterotoxins which cause food poisoning after ingestion. Causes wound infections, septicemia, endocarditis, meningitis, osteomylitis and pneumonia. Required for Hospital Disinfectants. Gram negative bacteria associated with acute gastroenteritis and septicemia. Required for Hospital Disinfectants. Gram negative (spherical shape) bacteria. Occurs in soil, water and sewage. A nosocomial infection can cause septicemia, meningitis and urinary tract infections. Black mold, found in shower and dressing rooms. Environmental contaminant may also cause Aspergillosis. Gram negative (spherical shape) bacteria. Causative agent for puppy cough in dogs. Bordetella pertusis is the causative agent for whooping cough in children. Fungi, yeast. This organism exhibits dimorphism; exists both as fungi and yeast. Causes skin rashes. Common cause for diaper rash. Can infect both oral and vaginal cavities. Causes itching and discomfort. Once believed to be a large virus but later found to be a parasitic bacterium. Infections cause fever, malaise and hacking cough. Most infections are occupational; poultry workers and other keepers of birds. Gram negative bacteria spread by anal/oral route of infection. Associated with bacteremia, respiratory, wound and urinary tract infections. Gram negative bacteria spread by anal/oral route of infection. Associated with bacteremia, respiratory, wound and urinary tract infections. Gram negative (rod shape) bacteria. Causative agent of hoof rot in sheep, cattle and horses. 9 Microbiology, D. Kingsbury and G. Wagner Harwal Publishing 1990
8 Summary of Antimicrobial Efficacy - Etiology - (Continued) Pathogenic Microorganism Escherichia coli Legionella pneumophila Listeria monocytogenes Klebsiella pneumoniae Pasteurella multocida Proteus mirabilis Proteus vulgaris Salmonella enteritidis Salmonella schottmuelleri Salmonella typhi Salmonella typhimurium Serratia marcescens Shigella dysenteriae Shigella flexneri Description Gram negative bacteria spread by anal/oral route of infection, resulting in diarrhea outbreaks. Associated with urinary tract infections and bacteremia. Gram negative (rod shape) bacteria. Causative agent for legionnaire disease. First documented outbreak occurred in 1976 at Philadelphia American Legion convention. Gram positive (rod shape) bacteria. Considered a potent food pathogen. Found in raw meat and poultry. Infections can result in meningitis or sepsis. Gram negative bacteria associated with severe pneumonia, bacteremia and urinary tract infections. Gram negative (spherical shape) bacteria. Human infections are a result of an animal bite. Indigenous flora of many animals respiratory tracts. Gram negative (rod shape) bacteria. Highly motile bacteria. Opportunistic pathogen causes bacteremia, urinary tract infections, especially with the chronically ill. Gram negative (rod shape) bacteria. Highly motile bacteria. Opportunistic pathogen causes bacteremia, urinary tract infections, especially with the chronically ill. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea, the causative agent for typhoid fever. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative bacteria associated with urinary tract infections, meningitis and septicemia. Gram negative bacteria directly spread by anal/oral route of infection; indirectly (including food, hands, flies) spread by contaminated food and inanimate objects resulting in bacillary dysentery. Gram negative bacteria directly spread by anal/oral route of infection; indirectly (including food, hands, flies) spread by contaminated food and inanimate objects resulting in bacillary dysentery.
9 Summary of Antimicrobial Efficacy - Etiology - (Continued) Pathogenic Microorganism Shigella sonnei Streptococcus (Enterococcus) faecalis Trichophyton mentagrophytes Adenovirus Type 4 Canine Distemper Feline Leukemia Virus Herpes Simplex Type 1&2 HIV-1 (AIDS Virus) Influenza A/Hong Kong Hepatitis B virus (HBV) Hepatitis C virus (HCV) Human Corona Virus Rabies Respiratory Syncytial Virus Rubella Vaccinia Description Gram negative bacteria directly spread by anal/oral route of infection; indirectly (including food, hands, flies) spread by contaminated food and inanimate objects resulting in bacillary dysentery. Gram positive (Enterococci) bacteria causing hemolysis, urinary tract infections and endocarditis. Athlete's foot fungus. Found in shower and dressing rooms. Lipophilic (enveloped) DNA virus, (one of several) causative agent for colds and other respiratory ailments. Lipophilic (enveloped) RNA virus. Highly contagious among dogs causes fever, gastrointestinal and respiratory symptoms. Non-enveloped RNA virus. One of the causative agents of lyphosarcoma in cats. Lipophilic (enveloped) DNA virus may result in oral mucocutaneous lesions. Associated with most orofacial herpes and HSV encephalitis. Lipophilic (enveloped) RNA retrovirus. Human Immunodeficiency Virus. Known to be the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). Lipophilic (enveloped) RNA virus. Causative agent in viral flu. Causes flu epidemics in nearly 2 of every 3 years. Lipophilic (enveloped) DNA virus of the hepadnavirus family. Causitive agent of Hepatitis B (serum hepatitis), Major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer. It is an enveloped RNA virus in the flavivirdae family. Single stranded RNA containing virus causing respiratory infection in humans. From order Nidovirales and Family Coronaviridae. A member of the Rhabdoviridae family or RNA viruses. These bullet shaped viruses are enveloped by a lipid bilayer. The causative agent for rabies, an encephalitis that causes neuronal degeneration-- almost always fatal. A paramyxovirus type virus, lipophilic (enveloped). A causative agent of pneumonia and bronchiolitis in small children and infants. Highly contagious, transmitted by person-to-person contact. Lipophilic (enveloped) RNA togavirus. The causative agent of German measles. Lipophilic (enveloped) DNA poxvirus; causes poxvirus infections.
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