SUMMARY OF ANTIMICROBIAL ACTIVITY
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1 SUMMARY OF ANTIMICROBIAL ACTIVITY SPECTRUM HBV Hospital Disinfectant Description Spectrum HBV Neutral Disinfectant & Detergent is a broad spectrum, neutral ph, hard surface disinfectant. When used as directed, this product will deliver effective biocidal action against bacteria, fungi, and es. This formulation is a blend of a premium active ingredients and inerts: surfactants, chelates, and water. Biocidal performance is attained when this product is properly diluted at 2 oz. per gallon or 1:64. Spectrum HBV can be used to disinfect a wide variety of hard surfaces such as floors, walls, and countertops in hospitals, households, and institutions. Regulatory Summary Physical Properties EPA Registration No ph of Concentrate 11.5 ±0.2 Flash Point (PMCC) >200 F USDA Authorization None Specific 25 C % Quat (mol. wt.360.5) California Status Pounds per 8.7 % Volatile C Canadian PCP# None Canadian Din # None (rev. 4/2008 jcg)
2 Summary of Antimicrobial Test Results Spectrum HBV Neutral Disinfectant & Detergent is a "One-Step" Hospital Disinfectant, Virucide, Fungicide, Mildewstat, and Cleaner. Listed below, and in the following pages, is a summary of the Antimicrobial Claims and a review of the Antimicrobial Test Results. Disinfectant 10 minutes 5% 400 ppm as CaCO 3 Official Method of the AOAC, 14 Edition Use Dilution Method Organism ATCC# Use-Dilution Concentration Campylobacter jejuni Corynebacterium ammoniagenes Enterobacter aerogenes Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Listeria monocytogenes Pseudomonas Clinical Isolate aeruginosa Salmonella enterica Salmonella 6539 Typhi Salmonella schottmuelleri Shigella dysenteriae Staphylococcus aureus (MRSA) Staphylococcus Clinical Isolate aureus Streptococcus salivarius Hard Water Condition Contact Time Results 400 ppm 10 minutes 0/60, 0/60, 0/ ppm 10 minutes 0/60, 0/60, 0/60 Conclusion: All lots of Spectrum HBV effectively killed the above listed bacteria as specified in the test performance standards. Spectrum HBV meets EPA requirements for hard surface disinfectant claims in hospital and medical environments when diluted to active concentration in 400 ppm synthetic hard water, and in the presence of 5% organic soil.
3 Summary of Antimicrobial Test Results - (Continued) Mildewstat 10 minutes 5% 400 ppm as CaCO 3 Mildewstat (Mold and Mildew Control) - EPA - TSD Mildewstat on Hard Surfaces Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Aspergillus niger ppm 10, 10 0/10, 0/10 Conclusion: All lots of Spectrum HBV were effective against Aspergillus niger under the test conditions outlined in the EPA test performance standards described above. Spectrum HBV is an effective mildewstat for non-porous inanimate hard surfaces when diluted to active concentration in 400 ppm synthetic hard water and in the presence of 5% organic soil. Fungicide 10 minutes 5% 400 ppm as CaCO 3 Official Method of Analysis of the AOAC Fungicidal Test. Organism ATCC# Dilution Replicate Results s 5 Min 10 Min 15 Min Trichophyton mentagrophytes / Conclusion: All lots of Spectrum HBV effectively killed Trichophyton mentagrophytes as specified in the test performance standards. Spectrum HBV is an effective fungicide for non-porous inanimate hard surfaces when diluted to active concentration in 400 ppm synthetic hard water and in the presence of 5% organic soil.
4 Summary of Antimicrobial Test Results - (Continued) Virucide Varies 5% 400 ppm as CaCO 3 EPA Guidelines Organism Source of or ATCC# Host System; Cytopathic Effect Use-Dilution Concentration Herpes Simplex Type HSV-1; ATCC VR-733 VERO 1 cells; lytic cytopathic effect Herpes Simplex Type HSV-2; MS Strain VERO 2 cells; lytic cytopathic effect HIV-1 HTLV-IIIRF; NCI MT2 cells; lytic cytopathic (AIDS ) effect Influenza A/ ATCC 68-H3N2 MDCK cells; lytic cytopathic Hong Kong effect Hepatitis B Hepadna Primary Duck Hepaocytes Testing, Inc. (DHBV) No Cytopathic Effects Hepatitis C Bovine Viral Diarrhea MDBK Cells (HCV) Human Corona VR-740 Strain 229E MRC-5 Host Influenza A2/Japan Rubella Strain M-33 RK13 cells; cytopathic effect Rabies ATCC VR-138 Respiratory Syncytial ATCC VR-26 Vaccinia Strain IHD VERO Cells; lytic cytopathic effect Avian Influenza A H5N1 Avian Influenza A H9N2 Turkey/Wisconsin Avian Reo Avian Infectious ATCC VR-22 Bronchitis Bovine Viral Diahhrea Canine Adeno Canine Corono Canine Distemper ATCC-VR-256 Canine Parainfluenza Canine Parvo 2812 ppm (CPV) (8.0 oz./gal) Equine Arteritis Contact Time 4 Min. 10 min. 10 min.
5 Organism Source of or ATCC# Host System; Cytopathic Effect Use-Dilution Concentration Feline Calici Feline Infectious Peritonitis Feline Leukemia ATCC VR-717 Strain FL-237 Feline Panleukopenia Feline Picorna ATCC VR-649 Feline Rhinotrachetis Infectious Bovine ATCC VR-793 Rhinotracheitis Infectious Avian Larynotracheitis (IBR) Infectious Bronchitis Infectious Canine Hepatitis Minute of Mice 2812 ppm (Parvo) (8.0 oz./gal) Murine Parainfluenza type 1 Newcastle disease Porcine Parvo 2812 ppm Porcine Respiratory & Reporductive (PRRSV) Porcine Rota Pseudorabies Transmissible Gastroenteritis ATCC VR-135 ATCC VR-763 (8.0 oz./gal) Contact Time Conclusion: All lots of Spectrum HBV effectively inactivated the above listed es as specified in the test performance standards. Spectrum HBV meets EPA requirements for hard surface virucidal claims in hospital and medical environments when diluted to in 400 ppm A.O.A.C. synthetic hard water and in the presence of 5% organic soil.
6 Summary of Antimicrobial Efficacy - Etiology 1 Pathogenic Microorganism Pseudomonas aeruginosa Staphylococcus aureus Aspergillus niger Enterobacter aerogenes Escherichia coli Listeria monocytogenes Klebsiella pneumoniae Salmonella enterica Salmonella schottmuelleri Salmonella typhi Shigella dysenteriae Enterococcus faecalis Trichophyton mentagrophytes Canine Distemper Feline Leukemia Herpes Simplex Type 1 HIV-1 (AIDS ) Influenza A/Japan Hepatitis B (HBV) Hepatitis C (HCV) Description Gram negative bacteria identified as a major cause of hospital acquired (nosocomial) infections. Causes wound infections (especially burn), meningitis, pneumonia and eye infections. Required for Hospital Disinfectants. Gram positive bacteria identified as a major cause of hospital acquired (nosocomial) infections. Colonizes food and secretes enterotoxins which cause food poisoning after ingestion. Causes wound infections, septicemia, endocarditis, meningitis, osteomylitis and pneumonia. Required for Hospital Disinfectants. Black mold, found in shower and dressing rooms. Environmental contaminant may also cause Aspergillosis. Gram negative bacteria spread by anal/oral route of infection. Associated with bacteremia, respiratory, wound and urinary tract infections. Gram negative bacteria spread by anal/oral route of infection, resulting in diarrhea outbreaks. Associated with urinary tract infections and bacteremia. Gram positive (rod shape) bacteria. Considered a potent food pathogen. Found in raw meat and poultry. Infections can result in meningitis or sepsis. Gram negative bacteria associated with severe pneumonia, bacteremia and urinary tract infections. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea, the causative agent for typhoid fever. Gram negative bacteria directly spread by anal/oral route of infection; indirectly (including food, hands, flies) spread by contaminated food and inanimate objects resulting in bacillary dysentery. Gram positive (Enterococci) bacteria causing hemolysis, urinary tract infections and endocarditis. Athlete's foot fungus. Found in shower and dressing rooms. Also the causative agent of Ring Worm, a fungi that is transmitted through non-socomal contact (e.g. activity mats, wrestling mats, etc) Lipophilic (enveloped) RNA. Highly contagious among dogs causes fever, gastrointestinal and respiratory symptoms. Non-enveloped RNA. One of the causative agents of lyphosarcoma in cats. Lipophilic (enveloped) DNA may result in oral mucocutaneous lesions. Associated with most orofacial herpes and HSV encephalitis. Lipophilic (enveloped) RNA retro. Human Immunodeficiency. Known to be the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). Lipophilic (enveloped) RNA. Causative agent in viral flu. Causes flu epidemics in nearly 2 of every 3 years. Lipophilic (enveloped) DNA of the hepadna family. Causitive agent of Hepatitis B (serum hepatitis), Major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer. It is an enveloped RNA in the flavivirdae family.
7 Human Corona Rabies Respiratory Syncytial Vaccinia Single stranded RNA containing causing respiratory infection in humans. From order Nidovirales and Family Coronaviridae. A member of the Rhabdoviridae family or RNA es. These bullet shaped es are enveloped by a lipid bilayer. The causative agent for rabies, an encephalitis that causes neuronal degeneration-- almost always fatal. A paramyxo type, lipophilic (enveloped). A causative agent of pneumonia and bronchiolitis in small children and infants. Highly contagious, transmitted by person-to-person contact. Lipophilic (enveloped) DNA pox; causes pox infections.
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