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1 GenEon Lab Testing Reports Table 1. The virucidal efficacy of the solution generated by the provided Trio RX Unit (BCS ) unit against Human herpesvirus 1 Virus. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants (2005) and ASTM E1053 Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. Free Available Chlorine: 530ppm at ph 7.0. Viruses are enumerated by cell culture as Infectious Units (IU) University Oak, San Antonio, TX sales@geneontechnologies.com
2 Table 2. The bactericidal efficacy of the liquid generated by GenEon s On-Site Generators; Germicidal Spray Products as Disinfectants (2005) using a 30 second contact time with a ppm of between Sprayed Inoculated Slides (number of replicates tested) microorganism cfu/ml inoculated per slide # cfu/ml recovered from each of slides sprayed* Log10 Bacteriophage MS-2 Pseudomonas aeruginosa x 10 5 <.91 > % > x 10 5 <.91 > % >5.5 File: Geneon Human HIV -poliovirus virucidal efficacy BCS December doc
3 Table 3. The virucidal efficacy of the solution generated by the provided Trio RX Unit (BCS ) unit against Human Poliovirus 1 (CHAT) in a protein load inoculum. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants (2005) and ASTM E1053 Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. Free Available Chlorine: 500ppm at ph 7.0. Viruses are enumerated as Infectious Units (IU). Poliovirus served as a model for Human Immunodeficiency Virus (HIV) inactivation* replicates tested Contact Time (minutes) Control Slides # Each Sprayed Slide* Log % % 99.8% % Human Poliovirus 1 VR x % % % 99.96% % % % % File: Geneon Human HIV -poliovirus virucidal efficacy BCS December doc
4 Table 4. The virucidal efficacy of the solution generated by the provided Trio RX Unit (BCS ) unit against Bovine Diarrhea Virus 1. Test was conducted as per AOAC Official Method ; Germicidal Spray Products as Disinfectants (2005) and ASTM E1053 Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. Free Available Chlorine: 510 ppm at ph Viruses are enumerated by cell culture as Infectious Units (IU). replicates tested Contact Time (Seconds) Control Slides# Each Sprayed Slide* Log % % 99.98% % Bovine Diarrhea Virus 1 VR x 10 3 <0.2 >99.994% <0.2 >99.994% <0.2 >99.994% >99.994% >4.2 <0.2 >99.994% <0.2 >99.994% >99.994% >4.2 <0.2 >99.994% File: Geneon HSV-1, Enterovirus 69, and BDV virucidal efficacy BCS December doc
5 Table 5. The virucidal efficacy of Trio RX unit (BCS ) generated solution against Human Poliovirus 1 (CHAT) suspension following a 30 second contact. Test was conducted as per ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. Free Available Chlorine: 1268ppm. Viruses are enumerated as Infectious Units (IU). Poliovirus served as a model for Human Immunodeficiency Virus (HIV) inactivation* replicates tested Contact Time (seconds) Control Slides # Each Sprayed Slide* Log 10 < 1.0 > % Human Poliovirus 1 ATCC VR x 10 5 < 1.0 > % > % > 5.6 < 1.0 > % File: Geneon 30 second virucidal efficacy BCS April doc
6 Table 5. The virucidal efficacy of Trio RX unit (BCS ) generated solution against Murine Norovirus MNV-1 suspension following a 30 second contact period. Test was conducted as per ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. Free Available Chlorine: 1224 ppm. Viruses are enumerated as Infectious Units (IU). Norovirus served as a model for Hepatitis B virus* replicates tested Contact Time (seconds) Control Slides # Each Sprayed Slide* Log % Murine Norovirus 1 ATCC PTA x % % % File: Geneon 30 second virucidal efficacy BCS April doc
7 Table 6. The virucidal efficacy of Trio RX unit (BCS ) generated solution against Bovine Diarrhea Virus suspension following a 30 second contact. Test was conducted as per ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. Free Available Chlorine: 1264ppm. Viruses are enumerated as Infectious Units (IU). Bovine Diarrhea Virus served as a surrogate for Hepatitis C virus as per the US EPA protocols* replicates tested Contact Time (seconds) Control Slides # Each Sprayed Slide* Log 10 <0.2 > % Bovine Diarrhea Virus ATCC VR x 10 4 <0.2 > % > % >5.6 <0.2 > % File: Geneon 30 second virucidal efficacy BCS April doc
8 Table 7. The virucidal efficacy of Trio RX unit (BCS ) generated solution against Human Herpesvirus 1 (HSV) suspension following a 30 second contact. Test was conducted as per ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. Free Available Chlorine: 1264ppm. Viruses are enumerated as Infectious Units (IU). replicates tested Contact Time (seconds) Control Slides # Each Sprayed Slide* Log 10 <1.0 > % Human Herpesvirus 1 ATCC VR x 10 5 <1.0 > % > % >5.1 <1.0 > % File: Geneon 30 second virucidal efficacy BCS April doc
9 Table 8. The mycobacteriocidal efficacy of the liquid generated by the provided Trio RX (BCS ) 1 at 30 second contact time at 412 ppm. Challenge Mycobacterium terrea cfu/ml inoculated per slide # cfu/ml recovered from each of slides sprayed* Log 10 Slide 1 <0.45 Slide 2 < x 10 6 Slide > % > 5.8 Slide 4 <0.45 Slide 5 <0.45 File: Trio RX Disinfectant Mycobacterium terrea time kill report draft.doc
10 Table 9. The mycobacteriocidal efficacy of the liquid generated by the provided Trio RX (BCS ) 1 at 60 second contact time Challenge Mycobacterium terrea cfu/ml inoculated per slide # cfu/ml recovered from each of slides sprayed* Log 10 Slide 1 <0.45 Slide 2 < x 10 6 Slide > % > 6.2 Slide Slide File: Trio RX Disinfectant Mycobacterium terrea time kill report draft.doc
11 Table 10. The mycobacteriocidal efficacy of the liquid generated by the provided Trio RX (BCS ) 1 at 120 second contact time Challenge Mycobacterium terrea cfu/ml inoculated per slide # cfu/ml recovered from each of slides sprayed* Log 10 Slide 1 <0.45 Slide 2 < x 10 6 Slide > % > 5.7 Slide Slide 5 <0.45 File: Trio RX Disinfectant Mycobacterium terrea time kill report draft.doc
12 Table 11. The mycobacteriocidal efficacy of the liquid generated by the provided Trio RX (BCS ) 1 at 180 second contact time Challenge Mycobacterium terrea cfu/ml inoculated per slide # cfu/ml recovered from each of slides sprayed* Log 10 Slide 1 <0.45 Slide x 10 6 Slide 3 <0.45 > % > 6.5 Slide 4 NA** Slide 5 NA** File: Trio RX Disinfectant Mycobacterium terrea time kill report draft.doc
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