Physiological and biochemical effects of copper nanoparticles in bell pepper (Capsicum annum L.) plants Swati Rawat, Venkata L.R.

Transcription

1 Physiologicl nd biochemicl effects of copper nnoprticles in bell pepper (Cpsicum nnum L.) plnts Swti Rwt, Venkt L.R. Pullgurl, Youping Sun, Genhu Niu, Jose A. Hernndez-Viezcs, Jose R. Perlt-Vide, nd Jorge L. Grde-Torresdey

2 INTRODUCTION

3 Properties of Nnomterils Mterils with t lest 2 dimensions between 1 nm nd 100 nm. Size rnge between individul molecules nd the corresponding bulk mterils High surfce energy Quntum confinement High redox ctivity

4 Applictions of Copper NPs

5 Globl flows for Cu nd oxides of Cu (metric tons/yr) in 2010 Keller, Arturo A., Suznne McFerrn, Anstsiy Lzrev, nd Sngwon Suh. "Globl Life Cycle Releses of Engineered Nnomterils." Journl of Nnoprticle Reserch 15, no. 6 (2013): 1-17.

6 Appliction of NPs in griculture Hong, J., Perlt-Vide, J.R. & Grde-Torresdey, J. 2013, "Nnomterils in griculturl production: benefits nd possible threts?".

7 Bell pepper plnts (Cpsicum nnum L.) Cpsicum nnuum L. Chilli or pungent flvor ttributed to the chemicl cpsicin 8-methyl-N-vnillyl-6-nonemide Rich in nti-oxidnts like crotenoid, sugrs, vitmin C Averge consumption 10.6 lbs/person/ yer 46,500 cres of lnd cultivted to produce 1535 million lbs 60% of the ntions bell peppers grown by Cliforni Averge yield 33,000 lbs/cre

8 Bell pepper world production, yield, hg/h North Americ Centrl Americ South Americ Europe Afric Asi Oceni

9 Reserch objective Effect of contminnt prticle size on the growth nd physiologicl prmeters of bell pepper plnt Effect of exposure period (45 vs 90 dys) on the elementl concentrtion of plnts

10 METHODOLOGY

11 Soil Soil collected Socorro, TX (N , W , elevtion: 1,115 m sl). Soil chrcteriztion conducted on Mlvern Mstersizer Hybrid 2000G - Snd : 19.7 % - Silt : % - Cly : % Nturl soil : silty lom

12 Prepring pots in the l for seedling trnsplnttion

13 Plnt growth stges Seedlings growing Seedlings redy for trnsplnttion Plnts 10 dys post trnsplnttion Freshly trnsplnted seedlings

14 Plnt growth stges Plnts 30 dys post trnsplnttion Fully mtured plnts, 90 dys post trnsplnttion Plnts 45 dys post trnsplnttion, flowering Plnts 60 dys post trnsplnttion, fruiting

15 Gs exchnge mesurement: CIRAS-3 portle photosynthesis system

16 Hrvesting

17 Acid digestion nd smple nlysis on the ICP-OES

18 RESULTS

19 Comprison of the evpotrnspirtion (ET) between vegettive stge nd reproductive stge study Evpotrnspirtion mmol/m 2 /s 7 Evpotrnspirtion t 45 nd 90 dys 6 5 cd cd c 4 bcde cde e de 3 Veg. Rep Control 62.5nCu 62.5bCu 500nCu 500bCu Tretment Concentrtion (mg/kg)

20 Comprison of the stomtl conductnce between vegettive stge nd reproductive stge study Stomtl Conductnce mol/m 2 /s 400 Stomtl conductnce t 45 nd 90 dys b Veg. 150 Rep Control 62.5nCu 62.5bCu 500nCu 500bCu Tretment Concentrtion, mg/kg

21 Comprison of the photosynthesis between vegettive stge nd reproductive stge study Photosynthesis µmol/m 2 /s 16 Photosynthesis t 45 nd 90 dys b 8 6 Veg. Rep Control 62.5nCu 62.5bCu 500nCu 500bCu Tretment Concentrtion, mg/kg

22 mg of Cu/ kg dry wt of root Elementl nlysis, comprison between tretments nd controls 800 Root Cu, vegettive stge vs reproductive stge study c c bc c cd Veg. Rep. 200 cd d Control 62.5 ncu 62.5 bcu 500 ncu 500 bcu Tretment

23 mg of Cu/kg of stem dry weight Elementl nlysis, comprison between tretments nd controls 250 Stem Cu, vegettive stge vs reproductive stge study Veg. Rep. 50 b b b b 0 Control 62.5 ncu 62.5 bcu 500 ncu 500 bcu Tretment

24 mg of Cu/kg dry wt of lef Elementl nlysis, comprison between tretments nd controls 300 Lef Cu, vegettive stge vs reproductive stge study bc Veg. Rep. 50 c c c c 0 Control 62.5 ncu 62.5 bcu 500 ncu 500 bcu Tretment

25 mg of Cu/ kg dry wt of fruit Elementl nlysis, comprison between tretments nd controls 300 Fruit Cu, vegettive stge vs reproductive stge study Veg. Rep Control 62.5 ncu 62.5 bcu 500 ncu 500 bcu Tretment

26 Summry nd concluding remrks 45 dy study hd significntly higher evpotrnspirtion mesurements s compred the 90 dy study, except t 500 ncu tretment. The stomtl conductnce nd photosynthesis were significntly lower t 62.5 bcu tretment s compred to select other tretments t the concentrtions studied. For the 90 dy study, root Cu ws significntly high t both ncu nd bcu 500 mg/kg concentrtions compred to control. For the 45 dy study, root Cu ws significntly high t both bcu concentrtions compred to control. In the stem tissue, ncu significntly incresed the Cu concentrtion t the 90 dy time point compred to the 45 dy tretment intervl. The lef Cu ws significntly higher t 45 dy exposure period for bcu tretments s compred to the ncu ones. The concentrtion of Cu in the fruit tissue ws not significntly ffected under tretments.

27 Acknowledgements UCCEIN for funding the reserch nd the djoining sponsors Texs A&M Agrilife Reserch nd Extension Centre t El Pso, TX. University of Texs t El Pso L Mtes Fculty - Dr Youping Sun - Dr Genhu Niu - Dr Jose A. Hernndez - Dr Jose R. Perlt - Dr Jorge Grde Torresdy The SNO conference orgnizers

28 References Keller, Arturo A., Suznne McFerrn, Anstsiy Lzrev, nd Sngwon Suh. "Globl Life Cycle Releses of Engineered Nnomterils." Journl of Nnoprticle Reserch 15, no. 6 (2013): 1-17 Hong, Jie, Cyren M. Rico, Lijun Zho, Adeyemi S. Adeleye, Arturo A. Keller, Jose R. Perlt-Vide, nd Jorge L. Grde-Torresdey. "Toxic Effects of Copper-Bsed Nnoprticles Or Compounds to Lettuce (Lctuc Stiv) nd Alflf (Medicgo Stiv)." Environmentl Science: Processes & Impcts 17, no. 1 (2015): M, X., Geiser-Lee, J., Deng, Y. & Kolmkov, A. 2010, "Interctions between engineered nnoprticles (ENPs) nd plnts: phytotoxicity, uptke nd ccumultion", Science of the totl environment, vol. 408, no. 16, pp Smit, S., Gupt, S.K., Brtonov, A., Dusinsk, M., Gutleb, A.C. & Rhmn, Q. 2012, "Nnoprticles in the environment: ssessment using the cusl digrm pproch", Environmentl Helth, vol. 11, no. Suppl 1, pp. S13. Hong, J., Perlt-Vide, J.R. & Grde-Torresdey, J. 2013, "Nnomterils in griculturl production: benefits nd possible threts?". Dimkp, C.O., Ltt, D.E., McLen, J.E., Britt, D.W., Boynov, M.I. & Anderson, A.J. 2013, "Fte of CuO nd ZnO nno-nd microprticles in the plnt environment", Environmentl science & technology, vol. 47, no. 9, pp

29 Thnk you for the ttention. Questions?

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