100 μm. Axon growth cones. Tubulin (red) + scr (green)

Size: px
Start display at page:

Download "100 μm. Axon growth cones. Tubulin (red) + scr (green)"

Transcription

1 Supplementary Figures mirorna-9 regulates axon extension an ranhing y targeting Map1 in mouse ortial neurons Dajas-Bailaor, F., Bonev, B., Garez, P., Stanley, P., Guillemot, F., Papalopulu, N. a 2 μm 1 μm p Relative RNA expression 15 p iz 1 5 U6 Tuulin (re) + mir-1 (green) e Tuulin (protein) Tuulin (re) + sr (green) f Tuulin (protein) mir-9 mir-13 mir-1 RNA Sr RNA Axon growth ones A. Tuulin (re)/mir-9 (green) Supplementary Figure 1. FISH of mir-9 on mouse rain setions at E17, showing expression in the (a) progenitor layer of the thir ventrile an () iz: intermeiate zone + white matter; p: ortial plate () RT-PCR analysis of mir-9 an mir-13 levels, emonstrating the relatively high expression of mir-9 in ortial neurons in ulture. Data are shown as perent of U6 RNA expression (mean + SEM). (-e) FISH of mir-1 an sramle (Sr) proes together with tuulin protein immunolaelling. Neither mir-1 nor Sr proes show loalisation in neuronal projetions (f) FISH of mir-9 together with aetylate tuulin protein immunolaelling in the axon growth ones of ortial neurons. Nature Neurosiene: oi:1.138/nn.382

2 Cell numer (% of total) Sramle p 1 p 2 p 3 p 4 p > 5 p s GFP + sramle GFP + mir-9 (5 nm) ax s ax GFP + LNA mir-9i (5 nm) ax s Supplementary Figure 2. (a) Distriution of the numer of neurons with to >5 ranh points in ontrol vs sramle mir proe transfete groups. Data are expresse as mean + SEM. () Representative images of neurons transfete with egfp plus sramle mir proe, mir-9 preursor or LNA mir-9i. s: enrites; ax: axons. Nature Neurosiene: oi:1.138/nn.382

3 Relative Lu. Ativity (%) WT mut Lu-Map1 3 UTR GFP-Map1 3 UTR-WT ontrol mir-9 GFP (% of ontrol) Cell oy WT mut WT mut GFP-Map1 3 UTR GFP-Map1 3 UTR-mut 5 μm 5 μm GFP + MAP1B GFP MAP1B (protein) 12 mir-9 sramle 1 μm 1 μm 1 μm MAP1B (% of ontrol) μm 1 μm 1 μm sr mir-9 5 nm e OD (%) ontrol mir-9 25 nm mir-9 5 nm sramle LNA mir-9i MAP1B GAPDH f Relative Map1 RNA levels ontrol sr mir-9 5 nm mir-9 ontrol 1 nm LNA LNA mir-9i 5 nm Supplementary Figure 3. Map1 is a target for mir-9 in ortial neurons. (a) Expression of luiferase reporter (Lu-Map1 3 UTR), wil type (WT) or with mutate (mut) mir-9 ining site, together with ontrol or mir-9 preursor () Loalize levels of expression of the same WT an mutate onstruts ut tagge to GFP an overexpresse in ortial neurons. () Representative images of ortial axons expressing GFP-Map1 3 UTR-WT (left) or GFP-Map1 3 UTR-mut (right). All images were proesse in parallel with iential aquisition settings. () Immunofluoresent etetion an quantifiation of enogenous MAP1B protein (re) in GFP positive ells, o-transfete with either sramle or mir-9 preursor in ortial neurons. Arrowheas iniate the axon of a transfete neuron, note ownregulation of MAP1B signal when mir-9 preursor is transfete. (e) Western lot of enogenous MAP1B or GAPDH proteins from ortial neurons after transfetion with mir-9 preursor or mir-9 LNA inhiitor. (f) RT-PCR of Map1 RNA levels in ortial neurons after transfetion with mir-9 preursor an LNA mir-9 inhiitor. Data is shown as fol hange with respet to ontrols. For all graphs ata are expresse as mean + SEM. Statistial signifiane ompare to respetive ontrols was etermine with Stuent s t test ( p <.5). Nature Neurosiene: oi:1.138/nn.382

4 Relative mir-9 levels (FC of ontrol) Ctrl LNA LNA mir-9i 5 nm Map1-TP 5 nm Relative mir-9 levels (FC of ontrol) Sr nm 1 nm 3 nm mir-9 Relative luiferase expression (FC of ontrol) LNA mir-9i (5 nm) Map1-TP (5 nm) Lu-Map1 3 UTR Lu-Hes1 3 UTR Lu-TLX 3 UTR Lu-Oneut1 3 UTR Supplementary Figure 4. Relative mir-9 levels after transfetion with the (a) LNA mir-9i an Map1- TP or with () mir-9 preursor in 17.2 ells. Data was normalize to snrna U6 an expresse as fol hange with respet to the ontrol. Inhiition of mir-9 ereases mature mir-9 levels, while the Map1-TP has no effet. () Relative luiferase expression levels in ifferentiate 17.2 ells o-transfete with either LNA mir-9i or Map1-TP an respetive luiferase reporters (Map1, Hes1, TLX, Oneut1). Inhiition of mir-9 inreases the expression levels of all targets, while the Map1-TP only has an effet on the Map1 3 UTR reporter. Data was expresse as fol hange ompare to the ontrol. For all graphs ata are shown as mean + SEM. Statistial signifiane ompare to respetive ontrols was etermine with Stuent s t test ( p <.5; p <.1). Nature Neurosiene: oi:1.138/nn.382

5 Somal sie Axonal sie um Axon length (% of ontrol) Axon length (% of ontrol) μm.1 μm.5 μm PNA mir-9i Map1 Relative Luiferase Ativity (FC of ontrol) LNA mir-9i 5 nm PNA mir-9i.1 μm (ell oy sie) PNA mir-9i.1 μm Supplementary Figure 5. (a) Shemati representation an representative image of a mirofluii hamer evie with its fluiially isolate axonal an somal ompartments (re: axonal aetylate tuulin, green: somal otx-1). () Measurement of axonal length on the axon sie after inuation with the PNA mir-9i only in the somati sie of the evie. No effet is oserve. () Measurement of axonal length in normal ortial ultures after inuation with the PNA mir-9i. Data for axonal length is shown as mean + SEM. Statistial signifiane was etermine using Mann-Whitney Rank Sum test for omparison with respetive ontrols. (p <.5). () Inrease in relative Lu-Map1 3 UTR ativity in 17.2 ells following inuation with LNA mir-9i or PNA-miR9-i. Data are expresse as fol hange to ontrols (mean + SEM). Statistial signifiane was etermine using Stuent s t test for omparison with respetive ontrols (p <.5; p <.1). Nature Neurosiene: oi:1.138/nn.382

6 mir-9 ensity (% of ontrol) BDNF: Axon sie axon length (% of ontrol) mir-9 ensity (% of ontrol) BDNF: Cell oy sie axon length (% of ontrol) ng ml-1 1 ng ml-1 1 ng ml-1 1 ng ml-1 Supplementary Figure 6. (a-) Quantifiation of mir-9 ensity an axonal length as measure on the axonal sie of the mirofluii evie with BDNF applie only to the (a-) axon or (-) ell oy sie of the hamers. Data is shown as mean + SEM. For axonal length experiments statistial signifiane was etermine using Mann-Whitney Rank Sum test for omparison with respetive ontrols. (p <.5). For mir-9 ensity omparisons, statistial signifiane was etermine using Stuent s t test for omparison with respetive ontrols. (p <.5; p <.1). Nature Neurosiene: oi:1.138/nn.382

7 A. Tuulin (re)/gw182 (green)/dapi (lue) a 1 μm Cell oy GW-182 fluoresene (% of ontrol) ng ml-1 mir9 12 mir-9 ensity (% of ontrol) ng ml-1 Supplementary Figure 7. (a) Protein loalization of the RISC omplex omponent GW-182 to the ell oy an istal axon in ortial neurons. (-) Quantifiation of GW-182 an omplementary mir-9 stran fluoresene in the istal axon after stimulation with BDNF. No statistially signifiant hanges were oserve, iniating that the effet of BDNF is not ue to a non-speifi moifiation of mirna levels or mirna proessing mahinery. Data is shown as mean + SEM. Nature Neurosiene: oi:1.138/nn.382

% of Nestin-EGFP (+) cells

% of Nestin-EGFP (+) cells 8 7 6 3 Nestin CD % of Nestin-EGFP (+) ells 8 6 Nestin-EGFP Marge Nestin-EGFP Marge Expression levels of Expression levels of Tumorigeniity y stemness markers ifferentiation markers limiting ilution assay

More information

b PolyA RNA-seq c RNA-seq read distribution FPKM

b PolyA RNA-seq c RNA-seq read distribution FPKM a Repliate 2 (FPKM) Poly+ RN-seq 14 1-2 R2 =.998 1-4 -4-2 1 1 14 Repliate 1 (FPKM) Repliate 2 (FPKM) Poly RN-seq 14 1-2 R2 =.996 1-4 1-4 1-2 14 Repliate 1 (FPKM) RN-seq rea istriution Intron 12% 14% Intergeni

More information

Learned spatiotemporal sequence recognition and prediction in primary visual cortex

Learned spatiotemporal sequence recognition and prediction in primary visual cortex Supplementary Materials for Learned spatiotemporal sequene reognition and predition in primary visual ortex Jeffrey P. Gavornik and Mark F. Bear Howard Hughes Medial Institute Piower Institute for Learning

More information

Supplementary Information

Supplementary Information Supplementary Information Optial ontrolling reveals time-dependent roles for adult-orn dentate granule ells Yan Gu, Maithe Arruda-Carvalho 3,4, Jia Wang, Stephen Janoshka,, Sheena Josselyn 3-5, Paul Frankland

More information

Rho-kinase Regulates Energy Balance by Targeting Hypothalamic Leptin Receptor Signaling

Rho-kinase Regulates Energy Balance by Targeting Hypothalamic Leptin Receptor Signaling Rho-kinase Regulates Energy Balane y Targeting Hypothalami Leptin Reeptor Signaling Hu Huang 1, Dong Kong 1, Kyung Hee Byun 2, Chianping Ye 1, Shuihi Koda 1, Dae Ho Lee 1, Byung-Chul Oh 2, Sam W Lee 3,

More information

Spontaneous persistent ac.vity in entorhinal cortex modulates cor.co- hippocampal

Spontaneous persistent ac.vity in entorhinal cortex modulates cor.co- hippocampal Spontaneous persistent a.vity in entorhinal ortex moulates or.o- hippoampal intera.ons in vivo Thomas T. G. Hahn, James M. MFarlan, Sven Bererih, Bert Sakmann, Mayank R. Mehta Neoortial persistent Up states

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION % ells with ili (mrke y A-Tu) Reltive Luiferse % ells with ili (mrke y Arl13) % ells with ili DOI: 1.138/n2259 A-Tuulin Hoehst % Cilite Non-ilite -Serum 9% 8% 7% 1 6% % 4% +Serum 1 3% 2% 1% % Serum: -

More information

Supplementary Material for. Inflammation induced repression of Foxp3-bound chromatin in regulatory T cells

Supplementary Material for. Inflammation induced repression of Foxp3-bound chromatin in regulatory T cells Supplementary Material for Inflammation inue repression of -oun hromatin in regulatory T ells Aaron Arvey 1,2, Joris van er Veeken 1,2, Roert M. Samstein 1,2, Yongqiang Feng 1,2, John A. Stamatoyannopoulos

More information

Cytomegalovirus and tumor stress-surveillance by human γδ T cell receptor binding to Endothelial Protein C Receptor

Cytomegalovirus and tumor stress-surveillance by human γδ T cell receptor binding to Endothelial Protein C Receptor Cytomegalovirus and tumor stress-surveillane y human γδ T ell reeptor inding to Endothelial Protein C Reeptor Carrie R. Willox, Vinent Pitard, Sonia Netzer, Lionel Couzi, Mahoo Salim, Toias Silerzahn,

More information

Supplemental Table 1. Primers used for real-time quantitative RT-PCR assay. Nucleotide sequence

Supplemental Table 1. Primers used for real-time quantitative RT-PCR assay. Nucleotide sequence Supplemental Table 1. Primers used for real-time quantitative RT-PCR assay Name FoxO1 forward FoxO1 reverse GK forward GK reverse INS-1 forward INS-1 reverse INS-2 forward INS-2 reverse Glut2 forward Glut2

More information

100 osimertinib. concentration of drugs (nm) Ba/F3 C797S/del19. concentration of drugs (nm) Ba/F3 C797S/T790M/L858R. relative cell vaibility (% of

100 osimertinib. concentration of drugs (nm) Ba/F3 C797S/del19. concentration of drugs (nm) Ba/F3 C797S/T790M/L858R. relative cell vaibility (% of Ba/F3 del19 Ba/F3 L858R a afatini afatini EGF-816 EGF-816 ty (% of ontrol) relative ell vaiilit elative ell vaiility (% of ontrol) 5 1 1 5 ty (% of ontrol) relative ell vaiilit 5 1 1 Ba/F3 T79M/L858R Ba/F3

More information

Supplementary Figure 1. Verification of drug infusions into the IPN. a. Representative

Supplementary Figure 1. Verification of drug infusions into the IPN. a. Representative Supplementary Figure 1. Verifiation of drug infusions into the IPN. a. Representative neutral red-stained oronal setion from a mouse with a guide annula targeting the IPN. The guide annula sar is irled

More information

mir-7a regulation of Pax6 in neural stem cells controls the spatial origin of forebrain dopaminergic neurons

mir-7a regulation of Pax6 in neural stem cells controls the spatial origin of forebrain dopaminergic neurons Supplemental Material mir-7a regulation of Pax6 in neural stem cells controls the spatial origin of forebrain dopaminergic neurons Antoine de Chevigny, Nathalie Coré, Philipp Follert, Marion Gaudin, Pascal

More information

MicroRNA-494 promotes cancer progression and targets adenomatous polyposis coli in colorectal cancer

MicroRNA-494 promotes cancer progression and targets adenomatous polyposis coli in colorectal cancer Zhang et al. Moleular Caner (8) 7: DOI.86/s94-7-75- RESEARCH Open Aess MiroRNA-494 promotes aner progression and targets adenomatous polyposis oli in oloretal aner Ying Zhang, Lu Guo,, Yuhuan Li,, Gui-Hai

More information

a b c Supplementary Figure 1 R Shrm4 0 -/- -/- GABA B1b Wild type GABA B1a GABA B1a -GABA B1b mean intensity (a.u) Ponceau GABA B

a b c Supplementary Figure 1 R Shrm4 0 -/- -/- GABA B1b Wild type GABA B1a GABA B1a -GABA B1b mean intensity (a.u) Ponceau GABA B a b c 200 - H S1 P1 S2 P2 SYN TIF PSD Shrm4 95 - PSD95 34 - Synaptophysin 120-100 - Wil type GABA B1a -/- GABA B1b -/- -GABA B1a -GABA B1b e f 5 μm 10 μm 20 μm 100 - GABA B1a 600 400 200 Ponceau GABA B

More information

FOXO3 signalling links ATM to the p53 apoptotic pathway following DNA damage

FOXO3 signalling links ATM to the p53 apoptotic pathway following DNA damage Reeive 19 Jan 212 Aepte 11 Jul 212 Pulishe 14 Aug 212 DOI: 1.138/nomms28 signalling links to the p apoptoti pathway following DNA amage Young Min Chung 1, *, See-Hyoung Park 1, *, Wen-Bin Tsai 2, Shih-Ya

More information

Supplementary Figure 1 - Ling

Supplementary Figure 1 - Ling HA1 (%) Insulin seretion (SI) e 7. p=....... 7 p=.7 1 7 a-ell granules -ell granules BMI (kg/m ) -ells / (a- + -ells) p=. 1 7 1. p=.1..... 7 Age: -ell ontent: % Age: -ell ontent: % Age: -ell ontent: %

More information

Supplementary Figure 1. Schematic illustrating major conclusions of this study.

Supplementary Figure 1. Schematic illustrating major conclusions of this study. ORNs GABA A GABA B glomeruli LN PNs Supplementary Figure 1. Shemati illustrating major onlusions of this study. This study represents the most diret evidene to date of inhiitory interations etween olfatory

More information

Tethering by lamin A stabilizes and targets the ING1 tumour suppressor

Tethering by lamin A stabilizes and targets the ING1 tumour suppressor Tethering y lamin A stailizes an targets the tumour suppressor Xijing Han 1,6, Xiaolan Feng 1,6, Jerome B. Rattner 2, Heather Smith 1, Pinaki Bose 1, Keiko Suzuki 1, Mohame A. Soliman 1,3, Mihelle S. Sott

More information

Chapter 2. Investigation into mir-346 Regulation of the nachr α5 Subunit

Chapter 2. Investigation into mir-346 Regulation of the nachr α5 Subunit 15 Chapter 2 Investigation into mir-346 Regulation of the nachr α5 Subunit MicroRNA s (mirnas) are small (< 25 base pairs), single stranded, non-coding RNAs that regulate gene expression at the post transcriptional

More information

Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish

Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Aute transriptional up-regulation speifi to osteoblasts/osteolasts in medaka fish immediately after exposure to mirogravity Masahiro Chatani, 1, Hiroya Morimoto,

More information

WT;ATTAC-5 ** ** SkM

WT;ATTAC-5 ** ** SkM SUPPLEMENTARY INFORMATION doi:.3/nature a WT;ATTAC- H/H;ATTAC- WT;ATTAC- H/H;ATTAC- 3 weeks months GFP p Inka relative expression 3 SkM Skel. Mus. FKBP- relative expression SkM Skel. Mus. relative expression

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Implication of Long noncoding RNAs in the endothelial cell response to hypoxia revealed y RNAsequencing. Voellenkle C., Garcia-Manteiga J. M., Pedrotti S., Perfetti A., De Toma

More information

An unconventional role for mirna: let-7 activates Toll-like receptor 7 and causes neurodegeneration

An unconventional role for mirna: let-7 activates Toll-like receptor 7 and causes neurodegeneration An unconventional role for mirna: let-7 activates Toll-like receptor 7 and causes neurodegeneration Sabrina M. Lehmann, Christina Krüger, Boyoun Park, Katja Derkow, Karen Rosenberger, Jan Baumgart, Thorsten

More information

Correspondence: mirna regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration

Correspondence: mirna regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration Correspondence: mirna regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration Alison A. Staton, Holger Knaut, and Antonio J. Giraldez Supplementary Note Materials and

More information

Supporting information

Supporting information Supporting information Evolution of Hollow TiO 2 Nanostrutures via the Kirkendall Effet Driven y Cation Exhange with Enhaned Photoeletrohemial Performane Yanhao Yu, 1 Xin Yin, 1 Alexander Kvit, 1,2 Xudong

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION -. -. SUPPLEMENTARY INFORMATION DOI: 1.1/ncb86 a WAT-1 WAT- BAT-1 BAT- sk-muscle-1 sk-muscle- mir-133b mir-133a mir-6 mir-378 mir-1 mir-85 mir-378 mir-6a mir-18 mir-133a mir- mir- mir-341 mir-196a mir-17

More information

SUPPLEMENTARY FIGURES AND TABLES

SUPPLEMENTARY FIGURES AND TABLES SUPPLEMENTARY FIGURES AND TABLES Supplementary Figure S1: CaSR expression in neuroblastoma models. A. Proteins were isolated from three neuroblastoma cell lines and from the liver metastasis of a MYCN-non

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy

More information

Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients.

Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients. Supplementary Materials Supplementary Figures Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients. Figure S2. Expression level of podocyte

More information

Mitosis. Single Nano Micro Milli Macro. Primary. PCNA expression

Mitosis. Single Nano Micro Milli Macro. Primary. PCNA expression a b c DAPI YFP CC3 DAPI YFP PCNA DAPI YFP ph3 DAPI YFP KI67 e 6 Mitosis f 1 PCNA expression %ph3 + /YFP + n= 63 87 61 3 13 8 n= 15 3 9 1 5 %PCNA+/YFP+ 8 6 Supplementary Figure 1. Proliferation/apoptosis

More information

Supplemental information

Supplemental information Supplemental information PI(3)K p11δ controls the sucellular compartmentalization of TLR4 signaling and protects from endotoxic shock Ezra Aksoy, Salma Taoui, David Torres, Sandrine Delauve, Aderrahman

More information

Supplementary information

Supplementary information Supplementary information Human Cytomegalovirus MicroRNA mir-us4-1 Inhibits CD8 + T Cell Response by Targeting ERAP1 Sungchul Kim, Sanghyun Lee, Jinwook Shin, Youngkyun Kim, Irini Evnouchidou, Donghyun

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION { OI: 1.138/n31 Srifie n nlyze APs on week 1 s of iet 1 4 6 High-ft iet BrU High-ft iet BrU 4 High-ft iet BrU 6 High-ft iet BrU Lin - Lin - : C34 + : C9 + 1 1 3 1 4 1 5 C45 1 C34 1 1 1 1 3 1 4 1 5 S-1

More information

Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1. Differential expression of mirnas from the pri-mir-17-92a locus.

Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1. Differential expression of mirnas from the pri-mir-17-92a locus. Supplementary Figure 1 Differential expression of mirnas from the pri-mir-17-92a locus. (a) The mir-17-92a expression unit in the third intron of the host mir-17hg transcript. (b,c) Impact of knockdown

More information

ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway

ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway Yang et al. Journal of Experimental & Clinial Caner Researh (2017) 36:170 DOI 10.1186/s13046-017-0643-9 RESEARCH Open Aess ALX4, an epigenetially own regulate tumor suppressor, inhiits reast aner progression

More information

Supplementary Figure 1. Quantile-quantile (Q-Q) plots. (Panel A) Q-Q plot graphical

Supplementary Figure 1. Quantile-quantile (Q-Q) plots. (Panel A) Q-Q plot graphical Supplementary Figure 1. Quantile-quantile (Q-Q) plots. (Panel A) Q-Q plot graphical representation using all SNPs (n= 13,515,798) including the region on chromosome 1 including SORT1 which was previously

More information

Supplementary Figure 1. mir124 does not change neuron morphology and synaptic

Supplementary Figure 1. mir124 does not change neuron morphology and synaptic Supplementary Figure 1. mir124 does not change neuron morphology and synaptic density. Hippocampal neurons were transfected with mir124 (containing DsRed) or DsRed as a control. 2 d after transfection,

More information

Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS)

Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS) Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS) and their exosomes (EXO) in resting (REST) and activated

More information

Supplementary Table 1. List of primers used in this study

Supplementary Table 1. List of primers used in this study Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3

More information

Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was

Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was painted on the shaved back skin of CBL/J and BALB/c mice for consecutive days. (a, b) Phenotypic presentation of mouse back skin

More information

A Normal Exencephaly Craniora- Spina bifida Microcephaly chischisis. Midbrain Forebrain/ Forebrain/ Hindbrain Spinal cord Hindbrain Hindbrain

A Normal Exencephaly Craniora- Spina bifida Microcephaly chischisis. Midbrain Forebrain/ Forebrain/ Hindbrain Spinal cord Hindbrain Hindbrain A Normal Exencephaly Craniora- Spina bifida Microcephaly chischisis NTD Number of embryos % among NTD Embryos Exencephaly 52 74.3% Craniorachischisis 6 8.6% Spina bifida 5 7.1% Microcephaly 7 1% B Normal

More information

IP: anti-gfp VPS29-GFP. IP: anti-vps26. IP: anti-gfp - + +

IP: anti-gfp VPS29-GFP. IP: anti-vps26. IP: anti-gfp - + + FAM21 Strump. WASH1 IP: anti- 1 2 3 4 5 6 FAM21 Strump. FKBP IP: anti-gfp VPS29- GFP GFP-FAM21 tail H H/P P H H/P P c FAM21 FKBP Strump. VPS29-GFP IP: anti-gfp 1 2 3 FKBP VPS VPS VPS VPS29 1 = VPS29-GFP

More information

Neocortex Zbtb20 / NFIA / Sox9

Neocortex Zbtb20 / NFIA / Sox9 Neocortex / NFIA / Sox9 Supplementary Figure 1. Expression of, NFIA, and Sox9 in the mouse neocortex at. The lower panels are higher magnification views of the oxed area. Arrowheads indicate triple-positive

More information

P. Mathijs Voorhoeve, Carlos le Sage, Mariette Schrier, Ad J.M. Gillis, Hans Stoop,

P. Mathijs Voorhoeve, Carlos le Sage, Mariette Schrier, Ad J.M. Gillis, Hans Stoop, Supplemental Data A Genetic Screen Implicates mirna-372 and mirna-373 As Oncogenes in Testicular Germ Cell Tumors P. Mathijs Voorhoeve, Carlos le Sage, Mariette Schrier, Ad J.M. Gillis, Hans Stoop, Remco

More information

DOI: 10.1038/ncb2210 b. ICAM1 ng ml -1 P = 0.0001 Small RNA (15-30nts) ng ml -1 Cell Lysate Exosome HDL Plasma HDL Normal Human HDL mirnas R = 0.45 P < 0.0001 Normal Human Exosome mirnas Figure S1. Characterization

More information

MicroRNA-182 is induced by IL-2 and promotes clonal expansion of

MicroRNA-182 is induced by IL-2 and promotes clonal expansion of 1 Supplemental Material 2 3 4 MicroRNA-182 is induced by IL-2 and promotes clonal expansion of activated helper T lymphocytes 5 6 7 8 9 10 11 12 Anna-Barbara Stittrich 1, Claudia Haftmann 1, Evridiki Sgouroudis

More information

Figure S1, Beyer et al.

Figure S1, Beyer et al. Figure S1, eyer et al. Pax7 Myogenin si sitrl Hoechst T = 72h 14 1.8.6.4.2 12 1 8 6 4 2 24h 48h 96h diff. sitrl siset1 212 72h diff. b1 td r t Se km MyH Vinculin Myogenin β-ctin Vinculin MW b1 ka td r

More information

microrna PCR System (Exiqon), following the manufacturer s instructions. In brief, 10ng of

microrna PCR System (Exiqon), following the manufacturer s instructions. In brief, 10ng of SUPPLEMENTAL MATERIALS AND METHODS Quantitative RT-PCR Quantitative RT-PCR analysis was performed using the Universal mircury LNA TM microrna PCR System (Exiqon), following the manufacturer s instructions.

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Asymmetrical function of 5p and 3p arms of mir-181 and mir-30 families and mir-142 and mir-154. (a) Control experiments using mirna sensor vector and empty pri-mirna overexpression

More information

SUPPLEMENTARY MATERIAL

SUPPLEMENTARY MATERIAL SYPPLEMENTARY FIGURE LEGENDS SUPPLEMENTARY MATERIAL Figure S1. Phylogenic studies of the mir-183/96/182 cluster and 3 -UTR of Casp2. (A) Genomic arrangement of the mir-183/96/182 cluster in vertebrates.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2566 Figure S1 CDKL5 protein expression pattern and localization in mouse brain. (a) Multiple-tissue western blot from a postnatal day (P) 21 mouse probed with an antibody against CDKL5.

More information

Autophagy enhances the presentation of endogenous viralantigensonmhcclassimoleculesduring HSV-1 infection

Autophagy enhances the presentation of endogenous viralantigensonmhcclassimoleculesduring HSV-1 infection Autophagy enhanes the presentation of enogenous viralantigensonmhclassimoleulesuring V- infetion Lu English, Magali Chemali, Johanne Duron, Christiane Roneau, Annie Laplante, Diane Gingras, Diane Alexaner,

More information

Supplementary Figures

Supplementary Figures Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein

More information

SUPPLEMENTARY LEGENDS...

SUPPLEMENTARY LEGENDS... TABLE OF CONTENTS SUPPLEMENTARY LEGENDS... 2 11 MOVIE S1... 2 FIGURE S1 LEGEND... 3 FIGURE S2 LEGEND... 4 FIGURE S3 LEGEND... 5 FIGURE S4 LEGEND... 6 FIGURE S5 LEGEND... 7 FIGURE S6 LEGEND... 8 FIGURE

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION . Norml Physiologicl Conditions. SIRT1 Loss-of-Function S1. Model for the role of SIRT1 in the regultion of memory nd plsticity. () Our findings suggest tht SIRT1 normlly functions in coopertion with YY1,

More information

Supplementary Information

Supplementary Information Supplementary Information EGFRL858R mutant enhances lung adenocarcinoma cell invasive aility and promotes malignant pleural effusion formation through activation of the CXCL12CXCR4 pathway MengFeng Tsai

More information

Supplementary Figure 1.

Supplementary Figure 1. Supplementary Figure 1. Female Pro-ins2 -/- mice at 5-6 weeks of age were either inoculated i.p. with a single dose of CVB4 (1x10 5 PFU/mouse) or PBS and treated with αgalcer or control vehicle. On day

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2607 Figure S1 Elf5 loss promotes EMT in mammary epithelium while Elf5 overexpression inhibits TGFβ induced EMT. (a, c) Different confocal slices through the Z stack image. (b, d) 3D rendering

More information

SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28

SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28 Supp. Fig. 1 a APC b APC ICOS ICOS TCR CD28 mir P TCR CD28 P T cell Tolerance Roquin WT SG Icos mrna T cell Autoimmunity Roquin M199R SG Icos mrna www.nature.com/nature 1 Supp. Fig. 2 CD4 + CD44 low CD4

More information

Santulli G. et al. A microrna-based strategy to suppress restenosis while preserving endothelial function

Santulli G. et al. A microrna-based strategy to suppress restenosis while preserving endothelial function ONLINE DATA SUPPLEMENTS Santulli G. et al. A microrna-based strategy to suppress restenosis while preserving endothelial function Supplementary Figures Figure S1 Effect of Ad-p27-126TS on the expression

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Supplementary Figure 1. Behavioural effects of ketamine in non-stressed and stressed mice. Naive C57BL/6 adult male mice (n=10/group) were given a single dose of saline vehicle or ketamine (3.0 mg/kg,

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus

More information

The Obesity Susceptibility Gene Carboxypeptidase E Links FoxO1 Signaling in Hypothalamic Pro opiomelanocortin Neurons with Regulation of Food Intake

The Obesity Susceptibility Gene Carboxypeptidase E Links FoxO1 Signaling in Hypothalamic Pro opiomelanocortin Neurons with Regulation of Food Intake Plum et al., Supplementary online material The Oesity Suseptiility Gene Caroxypeptidase E Links FoxO1 Signaling in Hypothalami Pro opiomelanoortin Neurons with Regulation of Food Intake Leona Plum, Hua

More information

Nature Genetics: doi: /ng.2995

Nature Genetics: doi: /ng.2995 Supplementary Figure 1 Kaplan-Meier survival curves of patients with brainstem tumors. (a) Comparison of patients with PPM1D mutation versus wild-type PPM1D. (b) Comparison of patients with PPM1D mutation

More information

Cos7 (3TP) (K): TGFβ1(h): (K)

Cos7 (3TP) (K): TGFβ1(h): (K) IP#2: IP#1: Totl Lystes luiferse tivity (K): 6-4 - (K): luiferse tivity luiferse tivity (K): 2 1 RL-: - + + + + + Sm4-3F: + - + + + + MYC-Sm3: - - - - + + TβRI-HA(T204D): - - - + - + α-ha Luiferse Ativity

More information

Supplementary Material

Supplementary Material Supplementary Material Summary: The supplementary information includes 1 table (Table S1) and 4 figures (Figure S1 to S4). Supplementary Figure Legends Figure S1 RTL-bearing nude mouse model. (A) Tumor

More information

Supplementary Figure 1

Supplementary Figure 1 A B D Relative TAp73 mrna p73 Supplementary Figure 1 25 2 15 1 5 p63 _-tub. MDA-468 HCC1143 HCC38 SUM149 MDA-468 HCC1143 HCC38 SUM149 HCC-1937 MDA-MB-468 ΔNp63_ TAp73_ TAp73β E C Relative ΔNp63 mrna TAp73

More information

Supplementary Figure 1 - Weiner

Supplementary Figure 1 - Weiner Supplementary Figure - Weiner Figure : Bioinformatis pipeline. Sequening of samples was performed on oth the Rohe 454 GS FLX+ instrument and the Illumina MiSeq instrument. Raw reads from 454 sequening

More information

a b c periosteum parietal bone bone marrow dura periosteum suture mesenchyme osteogenic front suture mesenchyme 1

a b c periosteum parietal bone bone marrow dura periosteum suture mesenchyme osteogenic front suture mesenchyme   1 coronary suture sagittal suture DOI: 10.1038/ncb3139 a b c e parietal bone suture mesenchyme parietal bone bone marrow ura ura ura f parietal bone ura suture mesenchyme bone g ura osteogenic front suture

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI: 10.1038/ncb2211 a! mir-143! b! mir-103/107! let-7a! mir-144! mir-122a! mir-126-3p! mir-194! mir-27a! mir-30c! Figure S1 Northern blot analysis of mir-143 expression dependent on feeding conditions.

More information

a b Pml F/F Prrx1-cre f 1.4 n.s.

a b Pml F/F Prrx1-cre f 1.4 n.s. a b Pml F/F Prrx1-re X Prrx1-re-Pml F/F Relative CFU-F numbers 2. Prrx1-Cre-PML +/+ n.s. d early passages e f 1.4 n.s. Adsorbane Adsorbane.8.6.4.2.8.6.4.2 1 2 3 4 days in ulture late passages 2 4 6 8 1

More information

Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table.

Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table. Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Tale. Type of file: VI Title of file for HTML: Supplementary Movie 1 Description:

More information

MII. Supplement Figure 1. CapZ β2. Merge. 250ng. 500ng DIC. Merge. Journal of Cell Science Supplementary Material. GFP-CapZ β2 DNA

MII. Supplement Figure 1. CapZ β2. Merge. 250ng. 500ng DIC. Merge. Journal of Cell Science Supplementary Material. GFP-CapZ β2 DNA A GV GVBD MI DNA CapZ β2 CapZ β2 Merge B DIC GFP-CapZ β2 Merge CapZ β2-gfp 250ng 500ng Supplement Figure 1. MII A early MI late MI Control RNAi CapZαβ DNA Actin Tubulin B Phalloidin Intensity(A.U.) n=10

More information

Chapter 7. Control and Coordination

Chapter 7. Control and Coordination Chpter 7 Control n Coorintion 1 Whih of the following sttements is orret out reeptors? Gusttory reeptors etet tste while olftory reeptors etet smell Both gusttory n olftory reeptors etet smell Auitory

More information

Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols

Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols used to differentiate mouse ESCs. (b) Representative

More information

Measurement of Dose Rate Dependence of Radiation Induced Damage to the Current Gain in Bipolar Transistors 1

Measurement of Dose Rate Dependence of Radiation Induced Damage to the Current Gain in Bipolar Transistors 1 Measurement of Dose Rate Dependene of Radiation Indued Damage to the Current Gain in Bipolar Transistors 1 D. Dorfan, T. Dubbs, A. A. Grillo, W. Rowe, H. F.-W. Sadrozinski, A. Seiden, E. Spener, S. Stromberg,

More information

Lesions of prefrontal cortex reduce attentional modulation of neuronal responses. and synchrony in V4

Lesions of prefrontal cortex reduce attentional modulation of neuronal responses. and synchrony in V4 Lesions of prefrontl ortex reue ttentionl moultion of neuronl responses n synhrony in V4 Georgi G. Gregoriou,, Anrew F. Rossi, 3 Leslie G Ungerleier, 4 Roert Desimone 5 Deprtment of Bsi Sienes, Fulty of

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Kif1a RNAi effect on basal progenitor differentiation Related to Figure 2. Representative confocal images of the VZ and SVZ of rat cortices transfected at E16 with scrambled or Kif1a

More information

The metabolic checkpoint kinase mtor is essential for IL-15 signaling during the development and activation of NK cells

The metabolic checkpoint kinase mtor is essential for IL-15 signaling during the development and activation of NK cells The metaoli hekpoint kinase mtor is essential for IL- signaling uring the evelopment an ativation of NK ells Antoine Marçais, Julien Cherfils-Viini 6,, Charlotte Viant 7,, Sophie Degouve, Séastien Viel,8,

More information

GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9!

GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9! Supplementary information a +KA Relative expression d! Tlr9 5!! 5! NSC Neuron Astrocyte Microglia! 5! Tlr7!!!! NSC Neuron Astrocyte! GFP/Sβ/! Iba/Hoechst Microglia e Hoechst/Iba/TLR9! GFP/Iba/GFAP f Brain

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature12215 Supplementary Figure 1. The effects of full and dissociated GR agonists in supporting BFU-E self-renewal divisions. BFU-Es were cultured in self-renewal medium with indicated GR

More information

SHREE ET AL, SUPPLEMENTAL MATERIALS. (A) Workflow for tumor cell line derivation and orthotopic implantation.

SHREE ET AL, SUPPLEMENTAL MATERIALS. (A) Workflow for tumor cell line derivation and orthotopic implantation. SHREE ET AL, SUPPLEMENTAL MATERIALS SUPPLEMENTAL FIGURE AND TABLE LEGENDS Supplemental Figure 1. Derivation and characterization of TS1-TGL and TS2-TGL PyMT cell lines and development of an orthotopic

More information

Supplemental information

Supplemental information Carcinoemryonic antigen-related cell adhesion molecule 6 (CEACAM6) promotes EGF receptor signaling of oral squamous cell carcinoma metastasis via the complex N-glycosylation y Chiang et al. Supplemental

More information

SUPPLEMENTARY MATERIAL

SUPPLEMENTARY MATERIAL SUPPLEMENTARY MATERIAL Divergent effects of intrinsically active MEK variants on developmental Ras signaling Yogesh Goyal,2,3,4, Granton A. Jindal,2,3,4, José L. Pelliccia 3, Kei Yamaya 2,3, Eyan Yeung

More information

Supplementary Figure S1

Supplementary Figure S1 Supplementry Figure S1 - UTR m - 3HA - 2-1 hgh - 1 Uiquitin *! *! lk distl promoter m K3R/ K121R-3HA UTR hgh founder lines - HA - - founder lines TG- E1 L A2 B1 F9 G6 H4 H6 B C D2 G1 H3 J2 L - 7 IP: lk

More information

TEB. Id4 p63 DAPI Merge. Id4 CK8 DAPI Merge

TEB. Id4 p63 DAPI Merge. Id4 CK8 DAPI Merge a Duct TEB b Id4 p63 DAPI Merge Id4 CK8 DAPI Merge c d e Supplementary Figure 1. Identification of Id4-positive MECs and characterization of the Comma-D model. (a) IHC analysis of ID4 expression in the

More information

well for 2 h at rt. Each dot represents an individual mouse and bar is the mean ±

well for 2 h at rt. Each dot represents an individual mouse and bar is the mean ± Supplementary data: Control DC Blimp-1 ko DC 8 6 4 2-2 IL-1β p=.5 medium 8 6 4 2 IL-2 Medium p=.16 8 6 4 2 IL-6 medium p=.3 5 4 3 2 1-1 medium IL-1 n.s. 25 2 15 1 5 IL-12(p7) p=.15 5 IFNγ p=.65 4 3 2 1

More information

LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb

LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb SUPPLEMENTARY INFORMATION Supplementl Figure 1 doi:10.1038/nture09742 Lterl 1.0 mm from midline mpfc BNST mpfc BNST Lterl 2.1 mm from midline LHA LHA Lterl 2.7 mm from midline SUPPLEMENTAL INFORMATION

More information

Supplementary Figure 1. Implants derived from human embryoid body preparations contain non-cardiac structures. In early studies, infarcted hearts

Supplementary Figure 1. Implants derived from human embryoid body preparations contain non-cardiac structures. In early studies, infarcted hearts a Supplementary Figure 1. Implants derived from human emryoid ody preparations ontain non-ardia strutures. In early studies, infarted hearts reeived ell preparations of low ardia purity (

More information

Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma

Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma Supplementary information for: Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma Rintaro Hashizume 1, Noemi Andor 2, Yuichiro Ihara 2, Robin Lerner 2, Haiyun

More information

Supplemental Information. Metabolic Maturation during Muscle Stem Cell. Differentiation Is Achieved by mir-1/133a-mediated

Supplemental Information. Metabolic Maturation during Muscle Stem Cell. Differentiation Is Achieved by mir-1/133a-mediated Cell Metabolism, Volume 27 Supplemental Information Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by mir-1/133a-mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster Stas Wüst,

More information

Nature Neuroscience: doi: /nn Supplementary Figure 1

Nature Neuroscience: doi: /nn Supplementary Figure 1 Supplementary Figure 1 EGFR inhibition activates signaling pathways (a-b) EGFR inhibition activates signaling pathways (a) U251EGFR cells were treated with erlotinib (1µM) for the indicated times followed

More information

Supplementary Figure 1. Confocal immunofluorescence showing mitochondrial translocation of Drp1. Cardiomyocytes treated with H 2 O 2 were prestained

Supplementary Figure 1. Confocal immunofluorescence showing mitochondrial translocation of Drp1. Cardiomyocytes treated with H 2 O 2 were prestained Supplementary Figure 1. Confocal immunofluorescence showing mitochondrial translocation of Drp1. Cardiomyocytes treated with H 2 O 2 were prestained with MitoTracker (red), then were immunostained with

More information

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel) Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory

More information

Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic

Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic B cells from WT Nfat2 +/+, TCL1 Nfat2 +/+ and TCL1 Nfat2

More information

Scaffold function of long noncoding RNA HOTAIR in protein ubiquitination

Scaffold function of long noncoding RNA HOTAIR in protein ubiquitination Yoon et al, page Scaffold function of long noncoding RNA HOTAIR in protein ubiquitination Je-Hyun Yoon,, Kotb Abdelmohsen, Jiyoung Kim, Xiaoling Yang, Jennifer L. Martindale, Kumiko Tominaga-Yamanaka,

More information

HIF-inducible mir-191 promotes migration in breast cancer through complex regulation of TGFβ-signaling in hypoxic microenvironment.

HIF-inducible mir-191 promotes migration in breast cancer through complex regulation of TGFβ-signaling in hypoxic microenvironment. HIF-inducible mir-9 promotes migration in breast cancer through complex regulation of TGFβ-signaling in hypoxic microenvironment. Neha Nagpal, Hafiz M Ahmad, Shibu Chameettachal3, Durai Sundar, Sourabh

More information

Differential modulation of Ca v 2.1 channels by calmodulin and Ca 2+ -binding protein Nature Publishing Group

Differential modulation of Ca v 2.1 channels by calmodulin and Ca 2+ -binding protein Nature Publishing Group Differential modulation of Ca v 2.1 hannels y almodulin and Ca 2+ -inding protein 1 Amy Lee 1, Ruth E. Westenroek 1, Françoise Haeseleer 2, Krzysztof Palzewski 1 3, Todd Sheuer 1 and William A. Catterall

More information

Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated

Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated with zvad-fmk (10µM) and exposed to calcium oxalate

More information