HPLC Application Notes
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1 HPLC Application Notes
2 Antibiotics. Ampicillin. Chloramphenicol Prestige C8 5µm, 50 x 4.6mm CAN/MeOH/(NH 4 )HPO 4 (0/0/70) UV at 0nm Injection Volume µl Amino Acids. L-Valine. L-Phenylalanine Polar Embedded C8, 50 x 4.6mm 0mM K HPO 4 / H PO 4 Injection Volume 5µl
3 Drugs and Metabolites Caffeine Metabolites. Theobromine. Theophylline. Caffeine Reliasil C8 5µm, 50 x 4.6mm ACN / Water (5/85) Injection Volume µl Antineoplastic and related compounds Aspirin 4. Uracil. 5-Fluorouracil. -Deoxyuridine 4. 5-Fluoro- -deoxyuridine Polar Embedded C8, 50 x 4.6mm 0mM NH 4 H PO 4 Injection Volume 5µl Reliasil C8 5µm, 50 x 4.6mm ACN / Water (60/40) Injection Volume µl
4 Nucleic Acids. Uracil. 5-Fluorouracil Polar Embedded C8, 50 x 4.6mm 0mM NH 4 H PO 4 Injection Volume 5µl Reliasil C8, 50 x 4.6mm 0% ACN / 0mM NH 4 H PO 4 0.ml/min Injection Volume µl Food Additives. Benzoic Acid Organic Acids. Fumaric Acid. Formic Acid. Acetic Acid 4. Butyric Acid Reliasil C8 5µm, 50 x 4.6mm ACN / Water (60/40) UV at 00nm Injection Volume µl Reliasil C8 5µm, 50 x 4.6mm ACN / Water (60/40) UV at 00nm Injection Volume µl
5 Steroids. Estriol. Cortisone. Testosterone. Progesteron. Estradiol Reliasil C8 5µm, 50 x 4.6mm ACN / Water (50/50) UV at 0nm Injection Volume µl Reliasil C8 5µm, 50 x 4.6mm ACN / Water (50/50) UV at 0nm Injection Volume 4µl Water Soluble Vitamins 4. L-Ascorbic Acid (Vitamin C). Nicotinic Acid. Pyridoxine (Vitamin B6) 4. Nicotinamine 4. L-Ascorbic Acid (Vitamin C). Nicotinic Acid. Pyridoxine (Vitamin B6) 4. Nicotinamine % B (0-5min) Injection Volume 6µl Emerald C8 5µm, 50 x 4.6mm A: 0mM KH PO 4 -K HPO 4 (ph 6.6) B: 0mM KH PO 4 -K HPO 4 (ph 6.6)/ACN (80:0) 0%B (0-5min); 0-00%B (5-0min, linear) 00% B (0-5min) Injection Volume 6µl Reliasil C8 5µm, 50 x 4.6mm A: 0mM KH PO 4 -K HPO 4 (ph 6.6) B: 0mM KH PO 4 -K HPO 4 (ph 6.6)/ACN (80:0) 0%B (0-5min); 0-00%B (5-0min, linear)
6 Water Soluble Vitamins. L-Ascorbic Acid (Vitamin C). Nicotinic Acid. Pyridoxine (Vitamin B6) 4. Nicotinamine. L-Ascorbic Acid (Vitamin C). Nicotinic Acid. Pyridoxine (Vitamin B6) 4. Nicotinamine 4 00% B (0-5min) Injection Volume 6µl Competitor : C8 5µm, 50 x 4.6mm A: 0mM KH PO 4 -K HPO 4 (ph 6.6) B: 0mM KH PO 4 -K HPO 4 (ph 6.6)/ACN (80:0) 0%B (0-5min); 0-00%B (5-0min, linear) % B (0-5min) Injection Volume 6µl Competitor : C8 5µm, 50 x 4.6mm A: 0mM KH PO 4 -K HPO 4 (ph 6.6) B: 0mM KH PO 4 -K HPO 4 (ph 6.6)/ACN (80:0) 0%B (0-5min); 0-00%B (5-0min, linear) Water Soluble Vitamins. Riboflavin. Rescorcinol ACN/Water (5/58/0.) Orosil ODS 5µm, 50 x 4.6mm A: ACN/Water/TFA (5/58/.0) B: ACN/Water/TFA (60/40/.0) 0-90% B (0-0min, linear), 90% (0-5min) Injection Volume 0µl
7 Others Isoamyl Alcohol, IPA, and Glycerol. Isoamyl Alcohol. IPA. Glycerol Butanol, Ethanol, and Glycerol. Butanol. Ethanol. Glycerol Reliasil Amine 5µm, 50 x 4.6mm ACN/Water (60/40) RI Injection Volume µl Reliasil Amine 5µm, 50 x 4.6mm ACN/Water (60/40) RI Injection Volume µl Ranse, and Lysozyme Orosil ODS 5µm, 50 x 4.6mm A: ACN/Water/TFA (5/85/.0) B: ACN/Water/TFA (60/40/.0) 0-90%B (0-0min, linear), 90% (0-5min) Flow rate Injection Volume µl
8 UHPLC. Benzene. Naphthalene. Flourine. Cortisone. Testosterone. Progesterone : Gazelle.7um C8 50 x.mm : Gazelle.7um C8 : Acetonitrile /Water (60/40) : Acetonitrile /Water : 0. ml/min : 0. ml/min Pressure Pressure. D-Norgestrel. 9-Norethindrone : Gazelle.7um C8 50 x.mm : Acetonitrile /Water (60/40) : 0. ml/min Pressure 0 psi
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