This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation
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1 This annex is valid from: to Replaces annex dated: Pieter Stuyvesantweg AC Leeuwarden The Netherlands Location(s) where activities are performed under accreditation Head Office Location Abbreviation/ location code Head Office Pieter Stuyvesantweg AC Leeuwarden The Netherlands Sampling A. Milk and milk s Taking samples liquid s in favour all microbiological methods FLOV0001 in accordance with NEN-EN-ISO 707 B. Surfaces Taking samples surfaces by using a swab or cloth in favour all microbiological methods C. Cheese Taking samples a sector or piece cheese in favour all microbiological methods D. Surfaces Taking samples surfaces using plates in favour the enumeration yeasts and moulds FLOV0002 in accordance with NEN-ISO FLOV0004 in accordance with NEN-EN-ISO 707 FLOV0005 This annex has been approved by the Board the Dutch Accreditation Council, on its behalf, J.A.W.M. de Haas Director Operations Dutch Accreditation Council RvA Page 1 12
2 E. Air Taking samples air using the Biotest RCS Plus Air Sampler in favour quantitative and qualitative microbiological methods F. Taking samples air using sedimentation plates in favour quantitative and qualitative microbiological methods FLOV0006 FLOV0008 G. Cheese, cheese likely s and butter Taking samples for processed cheese in favour all microbiological methods FLOV0024 in accordance with NEN-EN-ISO 707 H. Drink- and proceswater Taking samples water for Legionella analysis (the associated test is carried out structurally by another accredited laboratory) FLOV0028 in accordance with NEN-EN-ISO and NEN-EN-ISO Microbiological Analyses 1 Milk and milk s, Enumeration Micro-organisms at 30 C; Colony count technique FLMV0001 equivalent to NEN-EN- ISO Milk, milk s and environmental samples, Detection Salmonella presence/absence test by realtime PCR FLMV0004 equivalent to NEN-EN-ISO (AFNOR TRA 02/12 01/09) 3 Milk, milk s and environmental samples, Enumeration Enterobacteriaceae at 37 C; without confirmation; Colony count technique FLMV Milk and milk s Enumeration spores aerobic mesophilic spore forming bacteria at 37 C; Colony count technique FLMV0007 equivalent to NEN 6813 Dutch Accreditation Council RvA Page 2 12
3 5 Milk, milk s, powdery raw materials and Enumeration presumptive Bacillus cereus at 30 C; Colony count technique, bascillus cereus agar FLMV0008 equivalent to ISO 7932 (AFNOR BKR 23/06 02/10) 6 Milk and milk s Enumeration bacteria at 55 C; Colony count technique FLMV Enumeration thermoresistant bacteria at 30 C; colony count technique 8 Milk Detection spores Butyric acid bacteria at 37 C; MPN FLMV0011 equivalent to NEN 6807 FLMV0012 in accordance with NEN Milk and milk s Enumeration thermoresistant streptococci at 45 C; Colony count technique FLMV0015 equivalent to NEN Enumeration lactobacilli at 30 C; Colony count technique FLMV0016 equivalent to NEN Powdered s Detection Enterobacteriaceae; presence/absence test FLMV Milk, milk s and environmental samples Detection Enterobacteriaceae; presence/absence test (reference method) FLMV0239 in accordance with NEN-ISO Milk and milk s Detection coliforms at 30 C; Colony count technique FLMV0044 plating equivalent to NEN-ISO Enumeration coagulase positive staphylococci; colony count technique at 37 C, RPF agar FLMV0055 equivalent to NEN-EN-ISO Milk and milk s, Detection Staphylococcus aureus; presence/absence test, realtime PCR FLMV0064 Dutch Accreditation Council RvA Page 3 12
4 16 Non-powdery milk s, Enumeration coliforms and -glucuronidase positive E.coli at 37 C Colony count technique, Rapid E. coli agar FLMV0065 equivalent to NEN-ISO 4832 and NEN-ISO (AFNOR BRD 07/7 12/04 en BRD 07/08-12/04) 17 Milk and milk s Enumeration high thermoresistant bacteria at 30 C; Colony count technique FLMV Enumeration spores aerobic thermophilic spore forming bacteria at 55 C; Colony count technique FLMV0071 equivalent to NEN Milk, milk s and water, Enumeration spores sulfitreducing Clostridia at 30 C; after anaerobic incubation; Colony count technique FLMV Milk and milk s Enumeration yeast's & moulds at 25 C; Colony count technique FLMV0073 equivalent to NEN-ISO Milk and milk s Detection Listeria Monocytogenes; AL + RLM with confirmation BAX PCR, presence/absence test FLMV0134 equivalent to NEN-EN-ISO Milk s Detection β-glucuronidase positieve E. coli after accumalation, LMX, presence/absence test FLMV Milk powder, powdery raw materials, powdery infant food and environmental samples Detection Cronobacter spp; presence/absence test, confirmation PCR, biochemical FLMV0212 detection in accordance with NEN-EN- ISO 22964, conformation equivalent to NEN-EN-ISO Dutch Accreditation Council RvA Page 4 12
5 Organic Analyses 24 Dairy s, infant food, oils and fats Determination fatty acid composition (C4-C24, saturated-, (multiple) unsaturated fatty acids and trans fatty acids); gaschromatografy FLCV5007 dairy s in accordance with NEN-ISO 15885/IDF184 infant food, oils and fats (implementation in accordance with NEN- ISO 15885/IDF184) 25 Infant food Determination the content thiamine and the content vitamin B2; HPLCfluorscention FLCV5194 equivalent to NEN-EN and NEN- EN Vivinal GOS (powdered en syrup) and powdered infant food Determination Transgalactooligosaccharides (T-GOS); High Performance Anion Exchange Chromatography and Pulsed Amperometric Detection (HPAEC-PAD) FLCV5315 equivalent to AOAC Powdered and liquid infant food, condensed milk (enriched with vitamin A and fat (enriched with vitamin A) Determination Vitamin A content (alltrans-retinol) and vitamin E ( -tocerol); HPLC FLCV Milk and milk powder powered and liquid dairy s and infant food Determination aflatoxin M1; HPLCfluorescence FLCV0049 Milk and milk powder in accordance with NEN-EN-ISO 14501/IDF 171 powered and liquid dairy s and infant food in- house method (implementation in accordance with NEN- EN-ISO 14501/IDF 171) 29 Milk, cream and milk powder Determination lactose; HPLC-RI FLCV0422 in accordance with NEN-ISO 22662/IDF 198 Dutch Accreditation Council RvA Page 5 12
6 30 Powdered and liquid dairy s and infant food Determination lactose and sucrose; HPLC-RI FLCV0422 in- house method (implementation in accordance with NEN- ISO 22662/IDF 198) 31 Powdered and liquid infant food and dairy s Determination Vitamin D3 content (cholecalciferol); LC-MS/MS FLCV Powdered and liquid infant food and dairy s Determination melamine; LC-MS/MS FLCV0359 in accordance with NPR-ISO/TS / IDF/RM Powdered and liquid infant food Determination taurine; LC-MS/MS FLCV0191 equivalent to AOAC SMPR Powdered and liquid infant food Determination pantothenic acid (vitamin B5); LC-MS/MS FLCV0288 in accordance with AOAC Official Method and equivalent to NEN-ISO Powdered and liquid infant food, powdered and liquid dairy s and premix Determination total L-carnitine; LC- MS/MS FLCV0338 equivalent to AOAC SMPR Spectrometric Analyses (NIR, FTIR) 36 Milkpowders Determination fat, protein and dry matter content and freezing point; Near Infrared Reflection Spectrometry (NIR) FLCV5266 fat, protein and moisture in accordance with NEN-ISO 21543/IDF 201 acidity 37 Powdered infant food Determination components fat, protein, and moisture; Near Infrared Reflection Spectrometry (NIR) FLCV5266 powdered infant food Dutch Accreditation Council RvA Page 6 12
7 38 Whey powder Determination components fat, protein, moisture, ash and lactose content; Near Infrared Reflection Spectrometry (NIR) FLCV 5266 fat, protein, moisture and lactose in accordance with NEN-ISO 21543/IDF 201 ash 39 Milk Determination components fat, protein, lactose and dry matter content; Fourier Transform Infra-Red Spectroscopy (FTIR) 40 Evaporated milk Determination components fat, protein and dry matter content; Fourier Transform Infra-Red Spectroscopy (FTIR) 41 Cream Determination fat, protein and dry matter content and freezing point; Fourier Transformed Infrared Spectrometry (FTIR) 42 Whey Determination fat, protein and dry matter content; Fourier Transformed Infrared Spectrometry (FTIR) FLCV5318 equivalent to NEN-EN-ISO 9622 FLCV5318 equivalent to NEN-EN-ISO 9622 FLCV5318 equivalent to NEN-ISO 9622 FLCV5318 equivalent to NEN-ISO 9622 Inorganic Analyses (Metal Analyses) 43 Powdered and liquid milk s and infant food, and liquid rest s with destination raw material for animal feed Determination the content elements; microwave destruction and Inductive Coupled Plasma Atomic Emission Spectrometer (ICP-AES) sodium, potassium, calcium, magnesium, phosphor, iron, copper, zinc and manganese FLCV Compound feed for young animals and Determination the content copper; microwave destruction and Inductive Coupled Plasma Atomic Emission Spectrometer (ICP-AES) FLCV5302 Dutch Accreditation Council RvA Page 7 12
8 45 Dairy s and water Determination components aluminium, chrome, molybdenum, lead, antimony, arsene, cadmium, cobalt, tin, mercury, selenium, nickel and strontium; microwave destruction and Inductive Coupled Plasma Mass Spectrometer (ICP-MS) FLCV Compound feed for young animals and Determination components lead, arsene, cadmium and mercury; microwave destruction and Inductive Coupled Plasma Mass Spectrometer (ICP-MS) FLCV Powdered and liquid infant food, powdered and liquid dairy s and premix Determination (total) iodine; Inductive Coupled Plasma Mass Spectrometer (ICP-MS) FLCV0347 equivalent to NEN-EN and AOAC SMPR Inorganic Analyses (Wet Chemistry Analyses) 48 Milk and milk s, Determination nitrate content; segmented flow analysis FLCV5086 milk and milk s in accordance with NEN-EN-ISO /IDF remaining (implementation in accordance with NEN-EN-ISO /IDF 189-2) 49 Milk and milk s and liquid rest s with destination raw material for animal feed Determination nitrite content; segmented flow analysis FLCV5270 in accordance with NEN-EN-ISO /IDF Fats Determination FFA in fats; titrimetry FLCV5095 Dutch Accreditation Council RvA Page 8 12
9 51 Infant food, cheese and processed cheese, water, liquid rest s with destination raw material for animal feed and milk and milk powders Determination chloride content; potentiometric titration FLCV5151 infant food in accordance with NEN 6855 cheese and processed cheese in accordance with NEN-EN-ISO 5943/IDF 88 water equivalent to NEN 6476 liquid rest s with destination raw material for animal feed and milk and milk powders 52 Powdered and liquid infant food Determination vitamin C content; potentiometric titration FLCV5268 liquid infant food equivalent to AOAC powdered infant food 53 Cheese, processed cheese, whey cheese, liquid and powdered dairy s and liquid rest s with destination raw material for animal feed Determination ph; potentiometry FLCV5104 cheese, processed cheese and whey cheese in accordance with NEN 3775 liquid and powdered dairy s and liquid rest s with destination raw material for animal feed 54 Powered infant food and milk powders Determination moisture content (4 hr. 102ºC); drying oven FLCV5176 powdered infant food equivalent to LKR zuigenligenvoeding appendix IX (1994) milk powders equivalent to IDF 26A (1993) 55 Butter Determination moisture content; Pumice method FLCV5375 equivalent to NEN-EN-ISO / IDF80-1 Dutch Accreditation Council RvA Page 9 12
10 56 Sweetened condensed milk, cheese, processed cheese and liquid rest s with destination raw material for animal feed Determination dry matter content; sand method FLCV5038 sweetened condensed milk equivalent to ISO 6734/IDF 15 cheese and processed cheese equivalent to NEN-EN-ISO 5534/IDF4 liquid rest s with destination raw material for animal feed 57 Milk, sweetened condensed milk and liquid dairy s Determination dry matter content; glass fibre method FLCV5039 milk equivalent to NEN 6844 sweetened condensed milk equivalent to ISO 6734/IDF 15 liquid dairy s 58 Powdered milk s and powdered infant food and milk Determination ash content (3hr. 525 C);Thermo Graphic Analyzer FLCV5301 powdered milk s and powdered infant food equivalent to NEN 6810 milk 59 Powdered milk s Determination L-lactic acid content and lactates; enzymatic method FLCV Milk, milk powders and cheese Determination lactose content; Enzymatic method utilizing the galactose moiety the lactose FLCV5321 Dutch Accreditation Council RvA Page 10 12
11 61 Milk, milk s, powdery dairy s, infant food and cheese Determination fat content; Röse-Gottlieb FLCV5050 milk equivalent to NEN-EN-ISO 1211/IDF 1 milk-, whey-, buttermilk-and butter serum powder equivalent to NEN-EN-ISO 1736/IDF 9 evaporated and sweetened condensed milk equivalent to NEN-EN-ISO 1737/IDF 13 cream equivalent to NEN-EN-ISO 2450/IDF 16 skimmed- and buttermilk and whey equivalent to NEN-EN-ISO 7208/IDF 22 infant food equivalent to NEN-EN-ISO 8381/IDF 123 cheese and processed cheese equivalent to NEN-EN-ISO 1735/IDF 5 62 Milk and liquid dairy s Determination the content nitrogen and calculation the level crude protein content; Kjeldahl FLCV5080 milk equivalent to NEN-EN-ISO /IDF 20-1 liquid dairy s 63 Powdered milk s and powdered infant food Determination the content nitrogen and calculation the level crude protein content; Dumas method FLCV5192 powdered milk in accordance with NEN-EN-ISO 14891/IDF 185 powdered infant food 64 Sweetened condensed milk and powdered infant food Determination sucrose content; polarimetry FLCV5225 sweetened condensed milk equivalent to ISO 2911/IDF 35 powdered infant food Dutch Accreditation Council RvA Page 11 12
12 65 Milk, cream and milk with flavours Determination phosphatase activity; fluorescention FLCV5306 milk and milk with flavours in accordance with NEN-EN-ISO /IDF cream 66 Milk and cream Determination freezing point; thermistor cryoscope FLCV5081 milk equivalent to NEN-EN-ISO 5764/IDF 108 cream Sensory analyses 67 Powdered infant food Determination the sensorial quality; smell test SGLV0001 equivalent to ISO Determination the sensorial quality; taste test 69 Determination the sensorial quality; visual test (appearance) SGLV0002 equivalent to ISO SGLV0003 equivalent to ISO Dutch Accreditation Council RvA Page 12 12
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