Total ore pe Total ore din planul de învăţământ 63

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1 Denumirea disciplinei FIŞA DISCIPLINEI IMMUNOLOGY Anul de studiu II Semestrul * I/II Tipul de evaluare finală (E / V / C) E1 Regimul disciplinei (Ob obligatorie, Op opţională, F - facultativă) Ob Numărul de credite 6 Total ore de studiu Total ore pe Total ore din planul de învăţământ individual semestru Titularul disciplinei Conf. Dr. Petru Cianga * Dacă disciplina are mai multe semestre de studiu, se completează câte o fişă pentru fiecare semestru Total C** S L P Facultatea Departamentul Profilul Specializarea Medicina Stiinte Morfo-Functionale Medicina Medicina Generala Numărul total de ore (pe semestru) din planul de învăţământ ** C curs, L activităţi de laborator, P proiect sau lucrări practice Cunoştinţe minime necesare studenţilor pentru parcurgerea noţiunilor de curs/stagii/lucrări practice Cell and molecular biology, biochemistry, genetics, histology, phisiology, histology, microbiology. Obiectivele disciplinei Acquiring concepts regarding: - antigenicity and immunogenicity - description of the immune system components - description of immune humoral and cellular responses - description of molecules within the immunoglobulin superfamily - description of hypersensitivities - description of fundamental concepts of autoimmune processes and immunodeficiencies Programa analitică a cursului 1.Terminology 1.1 Important events in immunology development 1.2 Immunologic memory and vaccination 1.3 Instructive theories 1.4 Selective theories 1.5 General features of innate immunity 1.6 General features of adaptive immunity 1.7 Functionality of the immune system Nr. ore

2 2.Immunogenicity and antigen structure 2.1 Characteristics of antigenic determinants recognized by antibodies 2.2 Characteristics of antigenic determinants recognized by T cells 2.3 The relationship between an antigenic determinant recognized by a Th lymphocyte and an antigenic determinant recognized by a B lymphocyte, found on a proteic antigenic structure 2.4 Factors and conditions that influence the immunogenicity 2.5 Specificity and cross-reactivity; Clinical applications of cross-reactivity 3. Innate immunity natural barriers, cells and molecules 3.1 Natural barriers: anatomical and physiological elements 3.2 Phagocytosis; Endocytosis; Pinocytosis 3.3 Cells and receptors that promote phagocytosis; the concepts of PAMP and PRR 3.4 NK cell and non-specific extracellular cytotoxicity 3.5 The eosinophil and its role in immunity 3.6 Macrophages, APCs and their role in antigen processing and presentation 3.7 Mast cells their physiologic role and their involvement in pathology 3.8 Interferons 3.9 Acute phase proteins 3.10 Receptors of the innate immune system 4. Receptors for antigen 4.1 Receptor for antigen of B cell; Immunoglobulins 4.2 Antibody structure 4.3 Immunoglobulinic domain 4.4 Variable regions 4.5 Structure and role of constant regions 4.6 Functional properties of immunoglobulin classes 4.7 Receptor for antigen of T cell; 4.8 TCR αβ; CD3 complex-structure 4.9 Co-receptor molecules CD4 and CD Mechanisms of antigenic recognition of antigen and MHC by T cell 5. Genetics of antigen receptor 5.1 Light chain κ genes 5.2 Light chain λ genes 5.3 Heavy chain genes 5.4 Sources of diversity in immunoglobulin generation 5.5 Regulatory mechanisms in immunoglobulin gene expression 5.6 Isotype (class) switch 5.7 TCR gene organization; mechanisms involved in TCR diversity 5.8 Regulation of TCR gene rearrangement 5.9 Secondary type of receptor CD3γδ

3 6. Ontogeny of B and T lymphocyte 6.1 Primary lymphoid organs: thymus and bone marrow 6.2 Ontogeny of T lymphocyte 6.3 Mechanisms that induce self tolerance for T cell lymphocyte 6.4 Subsets of T cells 6.5 Ontogeny of B cell and stromal cell role in B cell ontogeny 6.6 Mechanisms that induce self tolerance in B cell lymphocyte 6.7 CD5+ B cells 6.8 Malignancies that involve B cells 6.9 γδ T lymphocytes 7 Major Histocompatibility Complex (HLA) 7.1 Cellular distribution of MHCI and MHCII 7.2 Histocompatibility genetics; sources of diversity in MHC; genic map 7.3 Characteristics and functions of MHC 7.4 HLA complex 7.5 Crystallographic structure of MHC molecules 7.6 Clinical importance of HLA phenotyping 8 Antigen processing and presentation 8.1 Antigen presenting cells 8.2 Antigen recognition and immune response 8.3 Identification and characterization of natural peptides associated with MHCI and MHCII 8.4 Peptides as parts in molecular structure of MHCI and MHCII 8.5 Synthesis and intracellular transport of MHC molecules 8.6 Sites of peptide generation and association with MHC 8.7 General features about pathways of MHC presentation 8.8 Biological consequences of simultaneous recognition MHC-peptide by T cell 9 B and T cell activation 9.1 Experimental study models for T cell activation 9.2 Requirements for Tcell activation initiation; co-stimulatory molecules 9.3 TCR signal transduction 9.4 Consequences of signal transduction; nuclear transcription factors 9.5 T cell proliferation regulation 9.6 T cell inactivation; Anergy induction 9.7 B cell activation 9.8 Signal transduction by mig 9.9 Early events stimulation in B cell activation 9.10 Antibody response to Ti antigens 9.11 Antibody response to Td antigen 9.12 Antigen presentation to Th cell 9.13 Th cell signals mediated by cell contact 9.14 Regulation of isotype expression 9.15 Production and B memory cells 9.16 B cell activation versus inactivation

4 10 Functions and mechanisms of action of B and T effector cells 10.1 In vivo functions of T citolytic cells 10.2 CTL and NK cells in bacterial, viral and parasitic infections 10.3 CTL and NK cells in tumoral immunity 10.4 NK cells receptors 10.5 CTL s in autoimmunity 10.6 Interactions lymphocyte-target cell 10.7 Lethal hit and lytic mechanisms induced by lymphocyte 10.8 Auto protective mechanisms of CTL 10.9 Detachment from the conjugate and CTL recycling Functional Th populations balance Th1/Th Memory lymphocytes Regulatory lymphocytes 11 Cytokines 11.1 The role of cytokines in humoral and cell mediated immunity 11.2 Cytokines families 11.3 Cytokines structure 11.4 Cytokines properties 11.5 Receptor families for cytokines 11.6 Signal transduction through cytokine receptors 11.7 Cross regulation of cytokines 11.8 Cytokines antagonists 11.9 Diseases induced though cytokines 12. Immune response; Inflammation; Lymphocyte traffic 12.1 Lymphoid organs and tissues structure, functions 12.2 Lymphocyte recirculation and homing CD Primary immune response; activation signals 12.4 Germinal center events 12.5 Accessory molecules involved in B-T cell interaction 12.6 Follicular dendritic cells and their involvement in immune response 12.7 Isotype switch and affinity maturation 12.8 Secondary immune response 12.9 Immunological memory Adhesion molecules; diapedesis Local and systemic acute inflammation; chronic inflammation

5 13. Complement system 13.1 Basal circuit of C3; discrimination self-non self 13.2 Alternative pathway of activation 13.3 Classical pathway; immune complexes role in complement activation 13.4 Membrane attack complex; postc3 pathway 13.5 Receptors for complement 13.6.Anaphylatoxins and receptors for anaphylatoxins; role in inflammation 13.7 Complement system regulation 13.8 The role of complement in infections; inflammatory reaction mediated by complement 13.9 Acute inflammatory reaction mediators and effectors Deficiencies of some complement system components; clinical consequences 14. Deviated immune response: hipersensitivities; types and general characteristics 14.1 Hipersensitivity type I mechanism, role of IgE and mast cells 14.2 Hipersensitivity type I clinical example 14.3 Hipersensitivity type II- mechanisms of tissue destruction 14.4 Hipersensitivity type II clinical examples 14.5Hipersensitivity type III implications in pathology 14.6Hipersensitivity type IV clinical variants and mechanisms of production 14.7 Hipersensitivity type IV role of T lymphocytes and cytokines produced by these cells Programa analitică lucrări practice/stagii 1. Antigenicity/immunogenicity: implications for the immunization process 2,5 hours 2,5 ore 2. Cross reactivity : practical applications 2,5 ore 2,5 hours 3. Classes and subclasses of immunoglobulines 2,5 ore 2,5 hours 4. Blood groups. ABO and Rh systems. 2,5 ore 2,5 hours 5. Ag-Ab interaction in agglutination reaction 2,5 ore 2,5 hours 6. Ag-Ab interaction in precipitation reaction 2,5 hours 7. Hibridoma, monoclonal antibodies 2,5 hours 8. Solid phase tests: RIA, RIST, RAST 2,5 hours 9. Solid phase tests: ELISA 2,5 ore 2,5 hours 10. Imunohistochemistry, imunofluorescence. 2,5 hours 11. Blot: Western blotting, Southern blotting. 2,5 hours 12. Flow-cytometry 2,5 hours 13. Cellular functionality tests 2,5 hours

6 14. Immunological investigation in immune pathology 2,5 hours Competenţe practice dobândite la finalul anului/semestrului - understanding the principles of immunological methods and their application in practice Ponderea în notare exprimată în La stabilirea notei finale se iau în considerare procente (Total = 100%) - răspunsurile la examen / colocviu (evaluarea finală): 50% - răspunsurile finale la lucrările practice de laborator 35% - testarea periodică prin lucrări de control 15% - activităţile gen teme / referate / eseuri / traduceri / proiecte etc. - alte activităţi (PRECIZAŢI) : colocviu Descrieţi modalitatea practică de evaluare finală E/V (de exemplu: lucrare scrisă (descriptivă şi / sau test grilă şi / sau probleme etc.), examinare orală, colocviu individual ori în grup, proiect, etc.) - oral examination - MCQ (50 questions) Estimaţi timpul total (ore pe semestru) al activităţilor de studiu individual pretinse studentului (completaţi cu 0 activităţile care nu sunt cerute) 1. Descifrarea şi studiul notiţelor de 14 curs 8. Pregătire prezentări orale 0 2. Studiu după manual, suport de 28 curs 9. Pregătire examinare finală Studiul bibliografiei minimale indicate Consultaţii 4. Documentare suplimentară în bibliotecă Documentare pe teren 0 5. Activitate specifică de pregătire SEMINAR şi / sau LABORATOR Documentare pe Internet 0 6. Realizare teme, referate, eseuri, traduceri, etc Alte activităţi Pregătire lucrări de control Alte activităţi... 0 TOTAL ore studiu individual (pe semestru) = minimum 100 ore - Bibliografie obligatorie: - Tak Mak, Mary E. Saunders: Primer to the Immune Response, 2011, Academic Press, Elsevier Bibliografie suplimentară pentru materia de curs şi lucrări practice Imunologie - Richard Goldsby, Thomas J. Kindt, Barbara Osborne : Kuby s Immunology, 5th Edition, 2002;

7 - Charles A. Janeway, Paul Travers, Mark Walport, J. Donald Capra: Immunobiology, 6th Edition, 2004; - David Male, Jonathan Brostoff, David B. Roth, Ivan Roitt: Immunology, 7th Edition, 2006; - Abul K. Abbas, Andrew H. Lichtmann: Cellular and Molecular Immunology, 5th Edition, Updated edition, 2005 Data completării: Semnătura coordonatorului de activitate didactică 2012