Parasitology&Mycology, 6-year course, 2013/2014

Transcription

1 Topic 1. Fungi of various organs ontocenosis 1. Mucor racemosus Fresenius, culture on solid and liquid Sabouraud medium - description of growth - microculture, ethanol fixed slide, Giemsa stained, magnification 100x, 400x or 600x - 2. Rhizopus nigricans Ehrenberg, culture on solid and liquid Sabouraud medium - description of growth - microculture, ethanol fixed slide, Giemsa stained, magnification 100x, 400x or 600x - 3. Aspergillus fumigatus Fresenius, culture on liquid and solid Sabouraud s medium - growth description - aspergilloma - microscopic slide, PAS-stained, magnification 600x - description 4. Candida krusei (Castellani, 1910) Berkhout culture on liquid and solid Sabouraud medium - growth description - microculture, ethanol fixed slide, cotton blue stained, magnification100x, 400x or 600x - 5. Candida glabrata (Anderson, 1917), Meyer et Yarrow, culture on liquid and solid Sabouraud medium - growth description - microculture, ethanol fixed slide, cotton blue stained, magnification100x, 400x or 600x 6. Candida dubliniensis Sullivan, culture on liquid and solid Sabouraud medium - growth description - microculture, ethanol fixed slide, cotton blue stained, magnification100x, 400x or 600x - microcultures in Nickerson's medium and rise extract agar, magnification100x, 400x or 600x - 7. Cryptococcus neoformans (Sanfelice, 1895), Vuillemin, culture on liquid and solid Sabouraud s medium- description of growth - culture on arbutin medium - description of growth - culture on Christensen's medium - description of growth - smear of cerebrospinal fluid in India ink, magnification 1000x 8. test API 20C AUX - presentation 9. Sample cases of mycoses - lung radiograph of patients with aspergilloma - slide presentation Knowledge required before attending laboratory: - fungal classification, structure and replication (Muray et al. chapter 5) - pathogenesis of fungal diseases (Muray et al. chapter 68) * primary pathogens/mycoses * opportunistic pathogens/mycoses 2. Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition Virella G., Microbiology and Infectious Diseases. NMS, 3rd ed., Williams and Wilkins, The Science of Review, ISBN

2 Topic 2. Fungi of skin ontocenosis and arthropods I. Fungi of skin ontocenosis 1. Malassezia furfur (Robin, 1853) Baillin culture on liquid and solid Sabouraud medium - growth description - microculture, ethanol fixed slide, cotton blue stained, magnification100x, 400x or 600x - 2. Scopulariopsis brevicaulis Besnier, culture on liquid and solid Sabouraud's medium - growth description - microculture - fixed slide, fuchsin-stained, magnification 100x, 400x or 600x - 3. Epidemophyton floccosum (Harz,1870) Lagneron and Milochevitch, culture on liquid and solid Sabouraud s medium growth description - microculture - fixed slide, fuchsin-stained, magnification 100x, 400x or 600x - 4. Trichophyton rubrum Sabouraud, culture on liquid and solid Sabouraud s medium - growth description - microculture - fixed slide, fuchsin-stained, magnification 100x, 400x or 600x 5. Trichophyton mentagrophytes Blanchard, culture on liquid and solid Sabouraud s medium - growth description - microculture - fixed slide, fuchsin- stained, magnification 100x, 400x or 600x - - hair perforation test - demonstration 6. Microsporum canis Rodin, culture on liquid and solid Sabouraud s medium - growth description - microculture - fixed slide, fuchsin- stained, magnification 100x, 400x or 600x 7. Demonstration of the dermatophytes on Mycoline medium. 8. Blastomyces sp., Histoplasma sp.; Sporotrichum sp - slides presentation II. Arthropods 10. Argas reflexus Fabricius, imago - microscopic slide, magnification 25x xodes ricinus Linnaeus, imago - microscopic slide, magnification 25x, 100x - - a tick after blood meal - macroscopic preparation - seeds of Ricinus communis - demonstration 12. Sarcoptes scabiei Linnaeus, imago - photograph demonstration 13. Ctenocephalides canis Curtis, imago - microscopic slide, magnification 25x Musca domestica Linnaeus, imago - macroscopic preparation- demonstration - pupa - macroscopic preparation - demonstration - larva - microscopic slide, magnification 100x - - eggs - microscopic slide, magnification 100x 15. Lucilia sericata Meigen, imago - macroscopic preparation- 16. Stomoxys calcitrans Linnaeus, imago - macroscopic preparation- demonstration 17. Myiasis - slides presentation, movies 18. Insect in nosocomial infections - slides presentation

3 10. Sample cases of mycoses and parasitoses Knowledge required before attending laboratory: - dermatophytes * epidemiology * morphology * clinical syndromes - arthropodes of medical importance * epidemiology * morphology * clinical syndromes 2. Buczek A., Parasitology for medical students, KOLIBER Lublin Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition Chomicz L., Foundation of Medical Parasitology. Compendium for Medical Students, Medical University of Warsaw Virella G., Microbiology and Infectious Diseases. NMS, 3rd ed., Williams and Wilkins, The Science of Review, ISBN Peters W., Pasvol G., Tropical Medicine and Parasitology, 5th ed., Mosby 2002, ISBN

4 Topic 3. Protozoa of various organs ontocenosis 1. Trichomonas vaginalis Donne, culture - fixed in methanol, stained by Giemsa-stained, magnification l000x - 2. Giardia lamblia Stiles, trophozoite - fixed slide, Giemsa-stained, magnification 100x,400x or 600x - - cyst - fixed slide, hematoxylin-stained, magnification 1000x 3. Trypanosoma cruzi Chagas, microscopic slide from mouse blood, fixed in 70 /o ethanol, Giemsa stained, magnification 400x or 600x, 1000x - 4. Leishmania tropica (Wright,1903) Luhe, amastigota form - smear from skin ulceration, microscopic slide, fixed in 70% ethanol, Giemsa-stained, magnification 400x or 600x - - promastigota form - culture, microscopic slide, fixed, Giemsa-stained, magnification 400x or 600x - 5. Entamoeba histolytica Schaudinn, trophozoite- fixed slide, trichrome-stained, magnification 1000x - - cyst - fixed slide, trichrome-stained, magnification 1000x 6. Naegleria fowleri Carter, microscopic slide fixed in ethanol, stained by trichromic method, magnification 400x or 600x, l000x - 7. Plasmodium falciparum (Welch, 1897) Schaudinn, thick blood smear - fixed microscopic slide, Giemsa-stained, magnification 1000x- 8. Toxoplasma gondii Nicolle et Manceux, microscopic slide from white mouse ascites, fixed in methanol, Giemsa-stained, magnification 1000x 9. Babesia microti Franca, microscopic slide, magnification 1000x Sample cases of parasitoses Knowledge required before attending laboratory: - parasites and their environments - parasites and host interactions - the most important sources of infection of men - protozoa of medical importance * epidemiology * morphology * clinical syndromes 2. Buczek A., Parasitology for medical students, KOLIBER Lublin Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition Chomicz L., Foundation of Medical Parasitology. Compendium for Medical Students, Medical University of Warsaw Peters W., Pasvol G., Tropical Medicine and Parasitology, 5th ed., Mosby 2002, ISBN

5 Topic 4. Helminths of various organs ontocenosis 1. Paragonimus westermani (Kerbert, 1878) Braun, slides presentation 2. Taenia saginata (Göze, 1782) Weinland, strobila - macroscopic slide, fixed in 4% formalin - - proglottid - microscopic slide, fixed in Bouin's liquid, alum-carmine-stained, magnification 25x - - eggs - microscopic slide, fixed in 4% formalin, unstained, magnification 100x, 400x or 600x - - cysticercus bovis - macroscopic preparation, fixed with 4% formalin demonstration 3. Taenia solium Linneus, scolex - slide - demonstration - proglottid - microscopic slide, fixed in Bouin's liquid, alum-carmine stained, magnification 25x 4. Echinococcus multilocularis (Leuckart, 1863) Vogel mature individual - microscopic slide, fixed in Bouin's liquid, alum-carmine-stained, magnification 25x, 100x slides presentation 5. Echinococcus granulosus Batsch, mature individual - microscopic slide, fixed in Bouin's liquid, alum-carmine-stained, magnification 25x, 100x - demonstration - protoscolex - microscopic slide, fixed in 70% ethanol, unstained, magnification 100x, 400x or 600x 6. Hymenolepis nana (von Siebold, 1852) Blanchard, l scolex with proglottids - microscopic slide, fixed in Bouin s liquid, alum-carminestained, magnification 25x - eggs - microscopic slide, fixed in 70% ethanol, unstained, magnification 100x, 400x or 600x - 7. Hymenolepis diminuta (Rudolphi, 1819), Blanchard, scolex - microscopic slide, fixed in Bouin s liquid, alum-carmine-stained, magnification 25x - - proglottid - microscopic slide, fixed in Bouin s liquid, alum-carmine-stained, magnification 25x - - eggs - microscopic slide, fixed in 70% ethanol, unstained, magnification 100x, 400x or 600x - 8. Necator americanus (Stiles, 1902) Stiles, adult worm - microscopic slide, fixed in 70% ethanol, unstained, magnification 25x demonstration 9. Toxocara sp. - adult worm - microscopic slide, fixed in 70% ethanol, unstained, magnification 25x demonstration - eggs - microscopic slide, fixed in 4% formalin, unstained, magnification 100x, 400x or 600x Anisakis marina Linnaeus, slides presentation 11. Mansonella sp. - microfilariae in peripheral blood smear- fixed microscopic slide, Giemsa-stained, magnification 100x- demonstration

6 12. Trichinella spiralis (Owen, 1835) Raillet, 1895: - larva in a human striated muscle specimen - microscopic slide, fixed in Bouin's liquid, hematoxylin- and eosin-stained, magnification 100x, 400x or 600x -demonstration - larva in a rat's striated muscle specimen - trichinoscopy method - magnification 100x - preparation and 13. Sample cases of parasitoses Knowledge required before attending laboratory: - parasites - their environments and host interactions - the lung flukes - cestodes and nematodes of medical importance * epidemiology * morphology * clinical syndromes 2. Buczek A., Parasitology for medical students, KOLIBER Lublin Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition Chomicz L., Foundation of Medical Parasitology. Compendium for Medical Students, Medical University of Warsaw Peters W., Pasvol G., Tropical Medicine and Parasitology, 5th ed., Mosby 2002, ISBN

7 Topic 5. Susceptibility of parasites and fungi to drugs 1. Testing of parasite susceptibility: - chart of the effect curve of metronidazole to Trichomonas vaginalis population; - CL 5o calculation. 2. Testing of fungi susceptibility: a. the agar diffusion method - calculation of MIC on the base of nystatin effect curve on Candida albicans growth; b. the dilution method - determination of nystatin MIC for Aspergillus niger growth on liquid Sabouraud medium; c. ATB Fungus test (bio Merieux, France) - demonstration; d. FUNGITEST (BIO-Rad, France) - demonstration; e. discs method (DHN PAN, Poland) - demonstration; f. E-test (AB Biodisc, Sweden) - description of the method. 3. Sample cases of mycoses and parasitoses Knowledge required before attending labolatory: - antifungal agents (Muray et al. chapter 70) - rules of treatment of mycoses - treatment of parasitic infections 2. Buczek A., Parasitology for medical students, KOLIBER Lublin Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition 2009

8 Books for Microbiology - Parasitology & Mycology Basic: 1. A. Buczek, Parasitology for medical students, KOLIBER Lublin Murray P.R., Rosenthal K.S., Pfaller M.A.: Medical Microbiology. MOSBY Elsevier, Sixth Edition 2009 Recommended: 1. L. Chomicz, Foundation of Medical Parasitology. Compendium for Medical Students, Medical University of Warsaw G. Virella, Microbiology and Infectious Diseases. NMS, 3rd ed., Williams and Wilkins, The Science of Review, ISBN W. Peters, G. Pasvol, Tropical Medicine and Parasitology, 5th ed., Mosby 2002, ISBN