MICROBIOLOGY BACTERIOLOGY III semester

Transcription

1 MICROBIOLOGY BACTERIOLOGY III semester Introduction in microbiology, bacterial taxonomy, general bacterial properties. Bacterial structures (capsule, cell wall). Pathogenicity and virulence. Normal bacterial flora and symbiotic associations Bacterial structures, biosynthesis and organization of bacterial cell (spore, flagelum, pilli, etc.). Sterilization, control of sterilization, disinfection. Specimen collection (examination of specimen from different organ systems). Specimen transport (package and labeling) Physiology of bacterial cells. Bacterial metabolism; nutrition requirements for growth and replication of bacteria. Detection of bacteria in specimens (microscopic examination, antigen detection and gene detection) Bacterial genetic material; genetic exchange in bacteria: transformation, conjugation and transduction. Methods for isolation and identification of bacteria Mechanism of action of antibacterial agents. Bacterial resistance to antibacterial agents. Antimicrobial susceptibility testing.

2 *** NERADNI DAN Virulence factor of bacteria (adhesive factors, invasive factors, toxins). Properties and medical importance of Staphylococcus spp. Biofim formation Properties of and medical importance of Streptococcus spp. and Enterococcus spp. Practical lesson 1 lesson Laboratory diagnosis of infections caused by Staphylococcus spp. Properties and medical importance Corynebacterium spp Properties and medical importance of of Clostridium spp. Laboratory diagnosis of infections caused by Streptococcus spp. and Enterococcus spp Properties and medical importance of Mycobacterium spp. Properties and medical importance of anaerobic non-spore-forming bacteria. Practical lesson 1 lesson Laboratory diagnosis of infections caused by anaerobic spore-forming and non-spore-forming bacteria Pproperties and medical importance of family Enterobacteriacea. Characteristics and medical imortance of Escherichia coli. characteristics and medical imortance of opportunistic enterobacteria. Practical lesson - 1 lesson Laboratory diagnosis of infections caused by Mycobacterium spp.

3 Bacterial zoonoses. Properties and medical importance of Bacillus spp Properties and medical importance of Gram negative non-fermentative bacteria. Properties and medical importance of Salmonella spp., Shigella spp. and Yersinia spp Properties and medical importance of Neisseria spp. and Moraxella spp. Properties and medical importance of Nocardia spp. and Rhodococcus spp. Laboratory diagnosis of infections caused by enterobacteria and Gram negative non-fermentative bacteria *** NERADNO Properties and medical importance of Haemophilus influenzae, HACEK group and Bordetella pertussis Properties and medical importance of Legionella pneumophila and Listeria monocytogenes Practical lesson 1 lesson Laboratory diagnosis of infections caused by Neisseria spp Properties and medical importance of genus Chlamydia, Chlamydophila, Mycoplasma, Ureaplasma. Properties and medical importance of Vibrio spp., Campylobacter spp. and Helicobacter pylori Lecture 2 lesson Properties and medical importance of Brucella spp. and Francisella spp. Properties and medical importance of genus Rickettsia, Bartonella, Erhlichia, and Coxiella.

4 Laboratory diagnosis of infections caused by Chlamydia, Chlamydophila, Mycoplasma, Ureaplasma. Colloquium Microbiology I First term , 15.00h Institute of Microbiology and Immunology Second term h Institute of Microbiology and Immunology IV semester I week Lecture I- Virology- 2 h -Prof. Tanja Jovanović Introduction to virology and general properties of viruses. Virion structures (genome, capsid, viral envelope) Criteria for viral classification. Seminar 3 lessons Properties and medical importance of spirochetes (Treponema, Borrelia and Leptospira). II week Lecture II Virology 2h prof. Maja Ćupić Viral replication (recognition and attachment to the target cell, penetration, uncoating, trancription, translation, replication of the genome, viral protein synthesis, assembly, release) Bacterial vaccines. Practical lesson 1 lesson Serologic tests in diagnosis of bacterial infections. III WEEK Lecture III-Virology 2h Prof. Tanja Jovanović Virus-host interaction. Cytolitic infection.the phenomenon of virus persistance. Various types of persistant infection (chronic, latent, «slow» viral infection). Pathogenesis of viral infection. Microorganisms and their products in food, water and enviroment; diseases caused by these microorganisms Practical Lessons Virology 2 lessons

5 The principles of laboratory diagnosis of viral infections.collection of specimens. IV WEEK Lecture IV-Virology 2h prof. Aleksandra Knežević Picornaviridae, Orthomyxoviridae i Paramyxoviridae, Coronaviruses Seminar-Virology 3 lessons Viral genetics. Mutations. Recombinations Viral interference, interferons and antiviral mechanisms of interferons. V WEEK Lecture V-Virology 2h prof. Maja Ćupić DNA viruses: Parvoviridae, Adenoviridae, Papillomaviridae, Polyomaviridae, Poxviridae Practical Lessons Virology 3 lessons Virus isolation in living sistem of cells (embrionated eggs, cell cultures, experimental animals) Identification of viruses based on morphology of viruses, viral antigens and viral nucleic acid VI WEEK Lecture VI-Virology 2 h -prof. Maja Ćupić Herpesviridae (HSV 1,2, VZV, CMV, EBV, HHV 6,7,8) Practical Lessons Virology 2 lessons Serological diagnoses of viral infections: types of serological reactions serodiagnosis Basic principles of antiviral therapy and problems of VII WEEK Lecture VII-Virology 2 h Prof. Ivana Lazarević Hepatitis viruses (HAV, HBV, HCV, HDV, HEV, HGV) Seminars- Virology 3 lessons Oncogenic viruses. Mechanism of DNA and RNA oncogenesis. Laboratory diagnosis of herpesviral infections VIII WEEK Lecture VIII- Virology 2 lessons- Prof. Maja Stanojević Human retoviruses: HTLV 1, 2, HIV Reoviridae, Caliciviridae, Coronaviride, F types of Adenoviride and other Small Round Gastrointestinal Viruses Seminars- Virology 3 lessons Laboratory diagnoses of viral hepatitis Viral vaccine IX WEEK Lectures 2lessons Prof. dr A. Džamić Introduction in medical parasitology and mycology.

6 Seminar- Virology 3 lessons Laboratory diagnoses of HIV/AIDS Viral Zoonoses and Arboviruses X WEEK Prof. dr A. Džamić Epidemiological and ecological characteristics of protozoal infections. Medical important arthropods. Practical lessons 3 lessons. Morphology and biology of medical important protozoa. Intestinal and urogenital protozoa: Entamoeba histolytica, Giardia lamblia, Cryptosporidium, Balantidium coli, Trichomonas vaginalis. XI WEEK Prof. dr A. Džamić /I. Čolović Čalovski Major protozoal tropical diseases Seminar 3 Lessons. Blood and tissue-dwelling protozoa (Trypanosoma, Leishmania, Toxoplasma, Plasmodium). Laboratory diagnosis of blood and tissue-dwelling protozoa. XII WEEK V. Arsić Arsenijević Epidemiological and ecological characteristics of helminth infections. Tropical helminth infections. Practical lessons 3 lessons. Intestinal helmints ( Ascaris, Trichuris, Enterobius, Strongyloides, Ancylostoma, Taenia, Diphyllobothrium, Hymenolepis). Laboratory diagnosis of intestinal helmints. XIII WEEK V. Arsić Arsenijević Morphology and biology of fungi. Epidemiological and ecological characteristics of fungal infections. Seminar 3 lessons. Tissue-dwelling helmints ( Trichinella, Toxocara, Echinococcus, Taenia). Laboratory diagnosis of tissue-dwelling helmints. Arachnoentomology: morphology and biology. Ektoparasites and vectors. XIV WEEK V. Arsić Arsenijević Dimorphic fungi, Zygomycetes. Mycoallergoses and Mycotoxicoses. Seminar 3 lessons. Fungi,common cause of primary infection of the skin and itsadnexa:dermatophytes (Trichophyton, Microsporum, Epidermophyton) and fungi of the genus Malassezia. Fungi,

7 common cause of superficial, invasive and/or opportunistic infections: yeasts(candida, Cryptococcus) and molds (Aspergillus, Penicillium, Fusarium). Pneumocystis. XV WEEK Doc. I. Čolović Čalovski Antiparasitic agents and antimycotics. Practical lessons 3 lessons. Laboratory diagnosis of fungal infections of the skin and its adnexa, mucous membranes,eye and ear. Methods for fungal identification. Fungal susceptibility testing. Laboratory diagnosis of invasive fungal infection:invasive Candidiasis and Invasive Aspergillosis Colloquium MICROBIOLOGY II 1st term , 14,00-14,45 Institute Lab. Room 1-Group 1 ; Lab.Room 2- Group 2 2 nd term , 14,00-14,45 Institute Lab. Room 1-Group1/Group 2 Course Director Prof. Maja Ćupić mcupic@med.bg.ac.rs

8 TIMETABLE 3 th Semester (academic year ) FRIDAY MICROBIOLOGY LabS & Seminars (Institute) 8:00-11:15 MICROBIOLOGY Lectures Head Building 13:00-14:30 TIMETABLE 4 th Semester (academic year ) FRIDAY MICROBIOLOGY (Head Building) Lectures 9:00-10:30 MICROBIOLOGY (Institute) Labs & Seminars 11:00-13:30 Course Director Prof. Maja Ćupić