ORIGINAL CONTRIBUTION. Corticomotoneuronal Integrity and Adaptation in Spinal Muscular Atrophy. (SMA) is a neurodegenerative
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1 ORIGINAL CONTRIBUTION Corticomotoneuronal Integrity and Adaptation in Spinal Muscular Atrophy Michelle A. Farrar, MBBS, FRACP; Steve Vucic, PhD, FRACP; Heather M. Johnston, MBChB, FRACP; Matthew C. Kiernan, DSc, FRACP Objective: To gain further insight into disease pathophysiologic process and potential adaptations through investigating whether cortical dysfunction or plasticity is a feature of spinal muscle atrophy (SMA). Design: Prospective, double-center study. Setting: Outpatient clinics and research institute. Participants: Clinical assessments, combined with threshold-tracking transcranial magnetic stimulation techniques, were completed in 11 genetically characterized patients with SMA. Main Outcome Measures: Clinical, functional, and neurophysiologic variables were compared between the 11 patients with SMA types and 3, healthy control participants, and 1 patients with amyotrophic lateral sclerosis (ALS) serving as disease controls. Results: Maximal motor-evoked potential amplitude as a percentage of the compound muscle action potential was significantly increased in patients with SMA compared with the healthy controls but was similar to that in ALS (SMA, mean [SE], 39.7%[.%]; ALS, 3.% [.%]; controls,.3% [.5%]; F=1.1; P.1). In contrast, short-interval intracortical inhibition (SMA, 1.%[1.6%]; ALS,.3%[1.%]; controls, 17.%[.3%]; F=11.; P.1) and cortical silent-period duration (SMA,.[9.] milliseconds; ALS, 1.7[5.] milliseconds; controls,.[3.7] milliseconds; F=.; P=.1), similar between SMA patients and healthy controls, were significantly larger when compared with the findings in ALS. Of relevance, peripheral disease burden as measured by the compound muscle action potential amplitude (SMA, 6.3[.] mv; ALS, 5.9[.] mv; controls, 11.[.5] mv; F=35.5; P.1) and Neurophysiological Index (SMA,.7[.]; ALS,.7[.1]; controls, 3.1[.]; F =1.; P.1), were significantly reduced in both SMA and ALS patients when compared with healthy controls. Conclusions: Taken together, findings from the present study suggest that despite spinal motoneuron degeneration there remains preservation of corticomotoneuronal function in SMA. The greater corticomotoneuronal projections to surviving spinal motoneurons likely represent an adaptive response to spinal motoneuron degeneration in SMA. Arch Neurol. 1;69():67-73 Author Affiliations: Neurosciences Research Australia, Randwick (Drs Farrar, Vucic, and Kiernan); Department of Neurology, Sydney Children s Hospital, School of Women s and Children s Health (Drs Farrar and Johnston), and Prince of Wales Clinical School (Dr Kiernan), University of New South Wales; and Western Clinical School, University of Sydney (Dr Vucic), Sydney, Australia. SPINAL MUSCULAR ATROPHY (SMA) is a neurodegenerative disorder of spinal motoneurons characterized clinically by muscle weakness and atrophy secondary to mutations in the survival motor neuron 1 gene (SMN1 [OMIM *635; GenBank U13). 1 Although SMA presents as a pure lower motoneuron disorder, the survival motor neuron (SMN) protein, encoded by the SMN1 and SMN (OMIM *6167; GenBank NM_75) genes, is expressed in the spinal cord and brain. Alterations in spinal circuitry, specifically, deafferentation of motoneurons, has been demonstrated to be an early pathophysiologic event in SMA and may contribute to motor dysfunction. There has been a general paucity of studies assessing the functional integrity of corticomotoneurons in SMA. Whether corticomotoneuronal dysfunction occurs in SMA has not been elucidated and remains of clear significance in developing a better understanding of disease pathophysiologic characteristics. It is accepted that cortical plasticity may develop following lower motoneuron injury due to poliomyelitis 3 and, for example, with spinal cord injury, and it appears to be a dynamic process. - Specifically, abnormalities of cortical activation, with downregulation of intracortical interneuronal circuits, have been well documented following spinal cord injury. In contrast, the potential reorganization of the structure, function, and connections of the corticomotoneuron in response to peripheral neurodegeneration, or neuroplasticity, 9 has also not been determined in SMA. Resolution of this issue may be of therapeutic significance for patients with SMA given that rehabilitation programs aimed at optimizing neuronal processing may improve motor function and thereby quality of life. Author Affil Neuroscienc Australia, Ra (Drs Farrar, Kiernan); De Neurology, S Hospital, Sch and Children (Drs Farrar a Prince of Wa (Dr Kiernan) New South W Clinical Scho Sydney (Dr V Australia. ARCH NEUROL / VOL 69 (NO. ), APR 1 67
2 Cortical function may be clinically assessed using threshold-tracking transcranial magnetic stimulation (TMS) techniques. 1 Separately, cortical dysfunction in the form of cortical hyperexcitability has been reported 11,1 as part of the process of neurodegeneration in amyotrophic lateral sclerosis (ALS), an adult-onset disorder of motoneurons that in part resembles SMA. 13 The development of cortical hyperexcitability in ALS was linked to neurodegeneration. In addition, cortical hyperexcitability appeared as a primary change before onset of clinical weakness in asymptomatic familial carriers of mutations involving the copper/zinc superoxide dismutase 1 (SOD1) gene. 1 Consequently, the present study used novel threshold-tracking TMS techniques to assess whether cortical plasticity was evident in SMA and whether the reduced expression of the SMN protein in the central nervous system of patients with SMA was associated with the development of cortical hyperexcitability or, alternatively, reduced excitability. METHODS PARTICIPANTS Prospective studies were undertaken in 11 adolescent and adult patients with SMA types and 3. Diagnosis was confirmed by genetic testing with homozygous deletions of exons 7 and in the SMN1 gene. By definition, patients with SMA type manifested weakness after 6 months of age and the maximum motor milestone was the ability to sit. Those with SMA type 3 had symptomatic onset after 1 months of age and attained the ability to walk unaided. 15 No patient was taking medications with central nervous system activity, for example, benzodiazepines or antidepressants, which are known to affect cortical excitability. 16 All patients gave informed consent or assent to the procedures, which were approved by the South Eastern Sydney and Illawarra Area Health Service Human Research Ethics Committee. Patients with SMA underwent clinical assessments of muscle strength using the Medical Research Council (MRC) sum score 17 that incorporates 7 muscles on both sides ( from the upper limbs and 3 from the lower limbs), with normal strength indicated by a total score of 7. The Spinal Muscular Atrophy Functional Rating Scale (SMAFRS) 1 was used to assess the functional status of patients, with a maximum score of 5 indicating normal function. Hand function was evaluated using the Triggs hand function score, 19 graded as follows:,normal; 1,mild to moderate hand weakness without impairment of dexterity;, weakness with significant impairment of dexterity (ie, difficulty with handwriting and buttoning clothes); and 3, marked weakness (major disability and loss of fine motor control). Muscle strength was clinically assessed using the MRC for the abductor pollicis brevis (APB) because this muscle was used for excitability testing. PERIPHERAL NERVE STUDIES Amplitude of the compound muscle action potential (CMAP) (peak to peak) was recorded from the APB muscle, as were distal motor and F-wave latencies, and the number of F- responses recorded in trials was expressed as F-wave frequency. The CMAP provided an index of motor activation capacity against which cortically evoked motor responses could be compared. The Neurophysiological Index (NI) was calculated according to an established formula : NI=CMAP amplitude (in millivolts) [F-wave frequency/distal motor latency (in milliseconds)]. CORTICAL EXCITABILITY AND TMS THRESHOLD TRACKING Cortical excitability studies were performed using reported threshold-tracking TMS techniques. 1 Briefly, a 9-mm circular coil was applied over the motor cortex and oriented to induce current flow in a posterior-anterior direction. The coil was adjusted until the optimal position for a motor-evoked potential (MEP) was obtained from the APB muscle. The site was marked to ensure constant placement of the coil, the coil position was fixed with a brace, and the patient s head was stabilized with cushioning. The setup was constantly monitored for movement throughout the experiment. Magnetic currents were generated by high-power magnetic stimulators that were connected to a device (BiStim; Magstim Company Ltd) that allowed conditioning and test stimuli to be independently set and delivered through one coil. The MEP was measured from peak to peak, and the thresholdtracking target was set to. mv, 1 which is the midpoint of the steepest portion of the logarithmic stimulus-response curve. All neurophysiologic variables were recorded with the target muscle at rest. The target muscle was closely observed for the presence of voluntary activity as recorded by surface electrodes and also was viewed on a monitor; the participants were asked to relax the target muscle if the examiner noted the appearance of any voluntary activity. Cortical excitability testing incorporated several measurements, from which various data could be extracted for analysis. 1. Resting motor threshold (RMT) was defined as the stimulus intensity required to produce and maintain the target MEP of. mv.. The stimulus-response curve was determined by increasing the intensity of the magnetic stimulus to the following levels: 6%, %, 9%, 1%, 11%, 1%, 13%, 1%, and 15% RMT. Three stimuli were delivered at each level of stimulus intensity and MEP amplitudes were averaged. The absolute MEP reflects the sum and interaction of upper and lower motoneuron activity. To determine the corticomotoneuronal contribution, the peak-to-peak amplitude of the maximum MEP was expressed as a percentage of the peak-to-peak maximal CMAP amplitude obtained with supramaximal stimulation of the median nerve (millivolts) and reflected the density of the upper motoneuron projections on the anterior horn cell. Although the stimulus-response curve was recorded in all patients, the maximum MEP amplitude (as a percentage) and minimum MEP onset latency (milliseconds), were used for group comparisons. 3. Central motor conduction time (in milliseconds) was calculated according to the F-wave method. 1. Cortical silent period was induced by a single-pulse TMS while the patients performed a weak voluntary contraction. The duration of the silent period was measured from the beginning of the MEP to the return of electromyographic activity. 5. The function of intracortical inhibitory and excitatory circuits was assessed by applying subthreshold-conditioning stimuli set to 7% RMT at increasing interstimulus intervals, similar to previously devised paired-pulse TMS thresholdtracking protocols. 1 Short-interval intracortical inhibition (SICI) was determined using sequential interstimulus intervals including 1., 1.5,.,.5, 3., 3.5,., 5., and 7. milliseconds. The SICI was measured as the increase in the test stimulus intensity required to evoke the target MEP and calculated off-line as follows 1,3 : SICI=[(conditioned test stimulus intensity RMT)/RMT] 1. ARCH NEUROL / VOL 69 (NO. ), APR 1 6
3 Table. Clinical Details for 11 Patients With SMA Types and 3 a Patient No. SMA Type Age, y SMA FRS Total MRC MRC APB Years Age at Presentation Disease Duration, y Triggs Hand Abbreviations: APB, abductor pollicis brevis; MRC, Medical Research Council; SMA, spinal muscular atrophy; SMAFRS, SMA Functional Rating Scale. a Muscle strength was clinically assessed using the MRC score for the sum of deltoid, biceps, wrist extensors, APB, iliopsoas, quadriceps femoris, and tibialis anterior on both sides (normal strength scores 7). The patients had a mean (SE) age of 1.9 (.) years, SMAFRS score of 36.3 (.1), total MRC score of 51.5, MRC APB score of. (.1), age at presentation of.5 (1.3) years, disease duration of 17.9 (.) years, and Triggs hand score of 1. (.). Intracortical facilitation was measured during interstimulus intervals of 1, 15,, and 3 milliseconds. Facilitation was measured as the decrease in the conditioned test stimulus intensity required to evoke the target MEP. Recordings of CMAP and MEP were amplified and filtered (3 Hz to 3 khz) using an alternating current amplifier (Grass ICP511; Grass-Telefactor, Astro-Med, Inc) and sampled at 1 khz using a 1-bit data acquisition card (PCI-MIO-16E-; National Instruments). Data acquisition and stimulation delivery (both electrical and magnetic) were controlled by QTRACS software (Institute of Neurology, Queen Square, London, England). DATA ANALYSIS Cortical excitability in patients with SMA was compared with that in age-matched healthy individuals serving as controls (1 males; age range, 1-36 years; mean, 6. years) and, as disease controls, 1 patients with clinically probable or definite ALS with limb-onset disease (56 males; age range, 5-7 years; mean [SE], 56.6 [1.] years; disease duration, 1.5 [.] years) as defined by the revised El Escorial criteria. Clinical staging of ALS was performed in all patients using the ALS- Functional Rating Scale Revised (ALSFRS-R; maximum score is when there is no disability), 5 MRC scores for assessment of muscle strength, and Triggs hand function score. The unpaired, 1-tailed t test and one-way analysis of variance were used to compare mean differences between groups. Statistical significance was set at P.5, and results are expressed as mean (SE). Correlations between neurophysiologic indexes and clinical measurements of disease severity were analyzed using the Spearman rank correlation test. RESULTS Clinical features for the patients with SMA are summarized in the Table. Clinical severity varied, ranging from mild to profound weakness, as did functional impairment, as reflected by the broad spectrum in MRC score (range, 3-66; median, 5) and SMAFRS score (range, -9; median, 1). At the time of the study, 5 patients (5% of the total cohort) were independently ambulant. All patients with SMA reported infrequent (less than twice a month) or no muscle cramps or fasciculations. Clinical examination of the hand revealed generalized muscle wasting in patients with SMA in contrast to preferential split-hand wasting involving the APB and first dorsal interosseous muscles that is characteristic in patients with ALS. Of further relevance, as expected, there were no upper motoneuron clinical features in patients with SMA in contrast to those with ALS. In patients with ALS, the mean score on the ALSFRS-R was 39.3 (.), the MRC evaluation using the APB was.3 (.1), and the Triggs hand score was 1 (.). PERIPHERAL NERVE STUDIES The CMAP amplitude (SMA, 6.3 [.] mv; ALS, 5.9 [.] mv; controls, 11. [.5] mv; F = 35.5; P.1) and NI (SMA,.7 [.]; ALS,.7 [.1]; controls, 3.1 [.]; F = 1.; P.1) were significantly reduced in both SMA and ALS patients when compared with healthy controls (Figure 1) and confirmed a similar degree of peripheral disease burden across patients with SMA and ALS. ASSESSMENT OF CORTICAL EXCITABILITY Reproducible MEPs were obtained in all patients with SMA. The central motor conduction time was similar across all groups (SMA, 5.6 [.3] milliseconds; controls, 6. [.3] milliseconds; ALS, 5.1 [.] milliseconds; F =.6; P =.). The RMT, defined as the TMS intensity required to produce and maintain the target MEP response, was also similar between the groups (SMA, 5.% [.9%]; controls, 55.6% [1.6%]; ALS, 57.% [1.1%]; F =.3; P =.6) and remained stable throughout the testing period, supporting minimal head or stimulator movement during the study. The magnetic stimulus-response curve was nonlinear in patients with SMA and healthy controls. There was no significant difference between single-pulse maximal MEP amplitude (SMA,.5 [.] mv; controls,. [.3] mv; ALS,.1 [.] mv; F =.; P =.). The MEP:CMAP ratio, which ARCH NEUROL / VOL 69 (NO. ), APR 1 69
4 ACMAP Amplitude, mv takes account of the lower motoneuron contribution, was significantly greater in patients with SMA when compared with healthy controls (SMA, 39.7% [.%]; controls,.3% [.5%]; F = 1.1; P.1, Figure ) but similar to those with ALS (3.% [.%]). The similar maximal MEP amplitude and RMT were important negative results, with the tracking targets the same between groups. Additionally, the paired-pulse TMS threshold-tracking protocol ensured that the interaction between conditioning and stimulus intensity were the same. CHANGES IN SICI The SICI, defined as an increase in the test stimulus intensity required to track a constant target MEP of. mv, was similar in the SMA and healthy control groups (9.9% [1.9%] Figure 1. Comparison of compound muscle action potential (CMAP) amplitude and Neurophysiological Index values between the 3 study groups. A, The CMAP amplitude was significantly reduced in patients with spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS) vs healthy control participants but comparable between the SMA and ALS groups. B, The Neurophysiological Index value was reduced in patients with SMA and ALS vs healthy controls but comparable between the SMA and ALS groups. *P.1. Limit lines indicate standard error. MEP:CMAP Ratio, % SMA BNeurophysiological Index Control Figure. The maximum motor-evoked potential (MEP) obtained during transcranial magnetic stimulation was expressed as a percentage of the compound muscle action potential (CMAP) amplitude recorded following electrical stimulation to determine the corticomotoneuronal contribution. The MEP:CMAP ratio was significantly increased in patients with spinal muscular atrophy (SMA) and those with amyotrophic lateral sclerosis (ALS) vs healthy control participants. *P.1. Limit lines indicate standard error. ALS vs 1.7% [1.%]; P =.36) but markedly greater when compared with that in the ALS group (1.5% [1.%]; P.1; Figure 3A). In addition, peak SICI at 1 millisecond (SMA, 6.6% [3.3%]; controls, 6.7% [1.%]; P =.9) and 3 milliseconds (SMA, 1.% [1.6%]; controls, 17.% [.3%]; P =.) was also similar in the SMA and healthy control groups but was significantly greater when compared with the ALS group (SICI of 1 millisecond: ALS, 1.6% [1.%]; F =.9;P =.9; SICI of 3 milliseconds: ALS,.3% [1.%]; F = 11.; P.1; Figure 3B). Following SICI, a period of intracortical facilitation developed between interstimulus intervals of 1 to 3 milliseconds. Intracortical facilitation was similar between the SMA and healthy control groups (.7% [1%] vs.1% [1%]; P =.3) but was significantly greater in the SMA group when compared with the ALS group (.9% [1.3%]; P =.; Figure 3C). Taken together, these findings suggest that cortical excitability was preserved in patients with SMA and have reaffirmed the presence of cortical hyperexcitability in ALS. The duration of the cortical silent period increased from to. (9.) milliseconds in patients with SMA as stimulus intensity increased from 6% to 15% RMT and was similar to that of healthy controls (from to. [3.7] milliseconds). The cortical silent period duration was significantly longer in patients with SMA vs those with ALS (ALS, to 1.7 [5.] milliseconds; F =.; P =.1). CORRELATION WITH CLINICAL MEASURES Combining clinical assessments of disease severity with neurophysiologic characteristics, the CMAP amplitude in SMA correlated with clinical measures of disease burden (Figure ), including MRC sum score (R =.; P =.), MRC score from the target APB muscle (R =.; P =.9), and the SMAFRS score (R =.7; P =.1). In contrast, clinical assessments of disease severity, disease duration, or age at onset did not significantly correlate with cortical excitability measures, including MEP and peak SICI in participants with SMA. As such, the correlation of clinical measures of disease severity with reduced CMAP in patients with SMA highlights the predominance of peripheral abnormalities in the pathophysiologic processes of SMA and the development of the clinical phenotype. COMMENT Using clinical and functional measures combined with threshold-tracking TMS techniques, the present study has identified degeneration of the lower motoneuron axis in SMA. Despite this process, there remained preservation of corticomotoneuronal function in patients with SMA, refuting any significant physiologic effects of reduced SMN protein expression within the central nervous system. These findings suggest that a unique pathophysiologic process underlies lower motoneuron degeneration in SMA, particularly when compared with ALS. Specifically, measures of cortical excitability, including resting motor threshold, SICI, intracortical facilitation, and cortical silent-period duration, were normal in SMA. Further- ARCH NEUROL / VOL 69 (NO. ), APR 1 7
5 AMean SICI From ISIs 1-7 ms, % BThreshold Change, % CMean ICF From ISIs 1-3 ms, % 1 15 SMA Control ALS Interstimulus Interval, ms Figure 3. Short-interval intracortical inhibition (SICI). In healthy control participants, SICI occurs between interstimulus intervals (ISIs) of 1 to 7 milliseconds. This is followed by intracortical facilitation (ICF), as indicated by a decrease in the test stimulus intensity. A, The SICI was comparable between patients with spinal muscular atrophy (SMA) and the healthy controls and significantly reduced in patients with amyotrophic lateral sclerosis (ALS) (*P =.1). B, The mean SICI, between ISIs 1 to 7 milliseconds, in patients with SMA was similar to that of controls but significantly greater vs patients with ALS (P =.1). C, The ICF was similar between patients with SMA and healthy controls but significantly smaller compared with patients with ALS ( P =.). Limit lines indicate standard error. more, the descending corticomotoneuronal output remains preserved in SMA; consequently, it is proposed that the greater contribution and projections onto the surviving spinal motoneurons likely represent adaptive neuroplasticity rather than the cortical hyperexcitability that develops in ALS. NEUROPLASTICITY IN SMA Adaptive changes involving motor pathways have been documented across a range of childhood neurologic disorders. 6- Potential mechanisms of this enhanced plasticity include low levels of -aminobutyric acid (GABA) A mediated intracortical inhibition 9 and a prolonged period of synaptic reorganization in addition to potential responses to disease or therapy. Given that subthreshold conditioning stimuli do not excite the spinal motoneuron, the changes in threshold observed during the paired-pulse threshold-tracking TMS paradigm may be regarded as solely intracortical in origin and reflect excitatory and inhibitory function. 3 Specifically, SICI is linked to the function of GABAergic intracortical interneurons. 31 Findings from the present study argue against ongoing enhanced intracortical plasticity in adolescents and adults with chronic SMA and reflect the clinical course, in which the greatest loss of strength occurs at disease onset, suggesting a considerable early loss of motoneurons followed by increasing stability of the surviving neurons with slow or no clinical deterioration. 3 The absolute MEP amplitude depends on the sum of complex interactions between the corticomotoneuron and spinal motoneuron. Accordingly, the MEP:CMAP ratio may be applied to determine the corticomotoneuronal contribution onto surviving spinal motoneurons. 33 Findings from the present study have established an increase in the MEP:CMAP ratio that in turn may suggest the presence of adaptive central plasticity in SMA; this is a novel finding. The finding of preserved corticomotoneuronal function in SMA may refute the development of cortical hyperexcitability, as occurs in the various phenotypes of ALS, 11 including the progressive muscular atrophy variant, 1 as a possible basis. A potential mechanism underlying this increase in the MEP:CMAP ratio may relate to an increased density of corticomotoneuronal projections onto the anterior horn cells, resulting in greater summation of excitatory postsynaptic potentials on the motoneuron and ultimately increased efficiency of motor output and thereby muscle strength Furthermore, the up front course of SMA, in which the most rapid decline appears initially, appears to coincide with the period of enhanced synaptic reorganization and plasticity during development. The absence of a significant correlation between MEP with disease duration or age at onset may further support the hypothesis that the timing of neurodegeneration is early and followed by increasing stability. Of further relevance, larger MEPs have been observed in affected muscles in adults who had developed poliomyelitis in early life, when the ability of the developing cortex to reorganize might be quite different from that of the adult cortex. 3 In further contrast, corticomotoneuronal plasticity has not been demonstrated in previous studies of distinct pure lower motoneuronal disorders in which neurodegeneration is initially clinically apparent in maturity, including studies in patients with spinobulbar muscular atrophy (Kennedy disease). 3 Neuromodulation of corticospinal axons by local neural circuits originating at either the level of the brainstem or spinal cord, as suggested by observations that anterior horn cell activity in SMA is less suppressed by exposure to GABA, 39 may also contribute to any observed plasticity. While this latter mechanism cannot be discounted, the finding of normal cortical silent-period duration, in part mediated by local spinal inhibitory circuits acting via GABA B receptors, 16 would seem to argue against this mechanism in patients with SMA, at least in the present cohort. Alterations in spinal circuitry, specifically, deafferentation of motoneurons, has been demonstrated as an early pathophysiologic event in SMA and may contribute to motor dysfunction. ARCH NEUROL / VOL 69 (NO. ), APR 1 71
6 ACMAP Amplitude, mv BCMAP Amplitude, mv MRC Sum Perhaps of further relevance, alterations in the intrinsic excitability of spinal motoneurons, thereby resulting in greater spinal motoneuron drive for a given input, 36 may also have contributed to the increased MEP:CMAP ratio. Supportive evidence of such a hypothesis is suggested by upregulation of persistent sodium conductances in SMA axons and spinal motoneurons, resulting in greater intrinsic motoneuronal excitability. 1 Technical factors in the form of aberrant muscle activity may have inadvertently contributed to an increase in MEP amplitude. Of note, muscle contraction results in an increase in MEP amplitude and shortening of MEP latency. However, this seems an unlikely explanation for an increased MEP amplitude in the present cohort of SMA patients because muscle activity was monitored during the testing procedure and the MEP latency was not significantly different from that of healthy and ALS controls. CLINICAL IMPLICATIONS y =.1x + 1. R =., P = SMAFRS y =.1x R =., P =.1 Figure. Correlation studies illustrating a relationship between clinical and peripheral neurophysiologic measures of disease severity in patients with spinal muscular atrophy, namely, compound muscle action potential (CMAP) amplitude. A, Medical Research Council (MRC) sum score. B, Spinal Muscular Atrophy Functional Rating Scale (SMAFRS). The solid lines represent the linear regression fit across all subjects. Spearman rank correlation coefficients and Bonferroni-corrected P values are shown. When taken in total, findings from the present series may tend to argue against any significant physiologic effects of reduced SMN protein expression within the central nervous system. These findings may further suggest that motoneuron degeneration in SMA is a process that is independent from glutamate excitotoxicity, in keeping with studies 3 that have established preservation of glial and neuronal glutamate transporters in SMA. This notion is supported by the clinical observation that the split hand, which refers to preferential atrophy of thenar and first dorsal interosseous muscles and is mediated by cortical mechanisms related to glutamate excitotoxicity, appears to be specific to ALS, contrasting with generalized atrophy of intrinsic hand muscles observed in SMA. In addition, the possibility of alterations in descending corticomotoneuronal pathways in SMA may be of therapeutic significance. Specifically, physical therapies commenced at an early age in individuals with SMA may enhance central adaptive processes, thereby potentially promoting stabilization and improvement of motor function. As such, pharmacologic therapies aimed at enhancing central plasticity, in particular the corticomotoneuronal pathway, may further complement physical therapies to improve motor function in SMA. Accepted for Publication: August, 11. Correspondence: Matthew C. Kiernan, DSc, FRACP, Neuroscience Research Australia, PO Box 1165, Randwick, Sydney, NSW 31, Australia (m.kiernan@unsw.edu.au). Author Contributions: Dr Farrar had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Farrar, Vucic, and Kiernan. Acquisition of data: Farrar and Vucic. Analysis and interpretation of data: Farrar, Vucic, Johnston, and Kiernan. Drafting of the manuscript: Farrar, Vucic, Johnston, and Kiernan. Critical revision of the manuscript for important intellectual content: Farrar, Vucic, and Kiernan. Statistical analysis: Farrar. Obtained funding: Kiernan. Administrative, technical, and material support: Vucic, Johnston, and Kiernan. Study supervision: Johnston and Kiernan. Financial Disclosure: Dr Kiernan serves as editor in chief of the Journal of Neurology, Neurosurgery & Psychiatry (BMJ Group). Dr Vucic serves on the scientific advisory board for Novartis, Merck Serono Australia, and Bayer Schering Australia and as a medical consultant for Merck Serono Australia. Funding/Support: Dr Farrar received grant support from the National Health and Medical Research Council of Australia: Medical Postgraduate Scholarship, ID REFERENCES 1. Lefebvre S, Bürglen L, Reboullet S, et al. Identification and characterization of a spinal muscular atrophy determining gene. Cell. 1995;(1): Mentis GZ, Blivis D, Liu W, et al. Early functional impairment of sensory-motor connectivity in a mouse model of spinal muscular atrophy. Neuron. 11;69 (3): Oliveri M, Brighina F, La Bua V, Buffa D, Aloisio A, Fierro B. Reorganization of cortical motor area in prior polio patients. Clin Neurophysiol. 1999;11(5): Levy WJ Jr, Amassian VE, Traad M, Cadwell J. Focal magnetic coil stimulation reveals motor cortical system reorganized in humans after traumatic quadriplegia. Brain Res. 199;51(1): Topka H, Cohen LG, Cole RA, Hallett M. Reorganization of corticospinal pathways following spinal cord injury. Neurology. 1991;1(): Lotze M, Laubis-Herrmann U, Topka H, Erb M, Grodd W. Reorganization in the ARCH NEUROL / VOL 69 (NO. ), APR 1 7
7 primary motor cortex after spinal cord injury a functional magnetic resonance imaging (fmri) study. Restor Neurol Neurosci. 1999;1(-3): Kokotilo KJ, Eng JJ, Curt A. Reorganization and preservation of motor control of the brain in spinal cord injury: a systematic review. J Neurotrauma. 9;6 (11): Lotze M, Laubis-Herrmann U, Topka H. Combination of TMS and fmri reveals a specific pattern of reorganization in M1 in patients after complete spinal cord injury. Restor Neurol Neurosci. 6;(): Cramer SC, Sur M, Dobkin BH, et al. Harnessing neuroplasticity for clinical applications. Brain. 11;13(pt 6): Vucic S, Howells J, Trevillion L, Kiernan MC. Assessment of cortical excitability using threshold tracking techniques. Muscle Nerve. 6;33(): Vucic S, Kiernan MC. Novel threshold tracking techniques suggest that cortical hyperexcitability is an early feature of motor neuron disease. Brain. 6;19 (pt 9): Vucic S, Kiernan MC. Abnormalities in cortical and peripheral excitability in flail arm variant amyotrophic lateral sclerosis. J Neurol Neurosurg Psychiatry. 7; 7(): Kiernan MC, Vucic S, Cheah BC, et al. Amyotrophic lateral sclerosis. Lancet. 11; 377(9769): Vucic S, Nicholson GA, Kiernan MC. Cortical hyperexcitability may precede the onset of familial amyotrophic lateral sclerosis. Brain. ;131(pt 6): Munsat TL, Davies KE. International SMA consortium meeting (6- June 199, Bonn, Germany). Neuromuscul Disord. 199;(5-6): Ziemann U. TMS and drugs. Clin Neurophysiol. ;115(): Medical Research Council. Aid to the Examination of the Peripheral Nervous System. Vol 1. London, England: Her Majesty s Stationary Office; Elsheikh B, Prior T, Zhang X, et al. An analysis of disease severity based on SMN copy number in adults with spinal muscular atrophy. Muscle Nerve. 9; (): Triggs WJ, Menkes D, Onorato J, et al. Transcranial magnetic stimulation identifies upper motor neuron involvement in motor neuron disease. Neurology. 1999; 53(3): de Carvalho M, Swash M. Nerve conduction studies in amyotrophic lateral sclerosis. Muscle Nerve. ;3(3): Claus D. Central motor conduction: method and normal results. Muscle Nerve. 199;13(1): Cantello R, Gianelli M, Civardi C, Mutani R. Magnetic brain stimulation: the silent period after the motor evoked potential. Neurology. 199;(1): Fisher RJ, Nakamura Y, Bestmann S, Rothwell JC, Bostock H. Two phases of intracortical inhibition revealed by transcranial magnetic threshold tracking. Exp Brain Res. ;13():-.. Brooks BR, Miller RG, Swash M, Munsat TL; World Federation of Neurology Research Group on Motor Neuron Diseases. El Escorial revisited: revised criteria for the diagnosis of amyotrophic lateral sclerosis. Amyotroph Lateral Scler Other Motor Neuron Disord. ;1(5): Cedarbaum JM, Stambler N, Malta E, et al; BDNF ALS Study Group (Phase III). The ALSFRS-R: a revised ALS functional rating scale that incorporates assessments of respiratory function. J Neurol Sci. 1999;169(1-): Carr LJ. Development and reorganization of descending motor pathways in children with hemiplegic cerebral palsy. Acta Paediatr Suppl. 1996;16: Carr LJ, Harrison LM, Evans AL, Stephens JA. Patterns of central motor reorganization in hemiplegic cerebral palsy. Brain. 1993;116(pt 5): Farmer SF, Harrison LM, Ingram DA, Stephens JA. Plasticity of central motor pathways in children with hemiplegic cerebral palsy. Neurology. 1991;1(9): Mall V, Berweck S, Fietzek UM, et al. Low level of intracortical inhibition in children shown by transcranial magnetic stimulation. Neuropediatrics. ;35 (): Kujirai T, Caramia MD, Rothwell JC, et al. Corticocortical inhibition in human motor cortex. J Physiol. 1993;71: Ziemann U. Cortical threshold and excitability measurements. In: Eisen A, ed. Clinical Neurophysiology of Motor Neuron Diseases: Handbook of Clinical Neurophysiology. Amsterdam, the Netherlands: Elsevier; : Farrar MA, Johnston HM, Grattan-Smith P, Turner A, Kiernan MC. Spinal muscular atrophy: molecular mechanisms. Curr Mol Med. 9;9(7): Weber M, Eisen AA. Magnetic stimulation of the central and peripheral nervous systems. Muscle Nerve. ;5(): Ashby P, Zilm D. Characteristics of postsynaptic potentials produced in single human motoneurons by homonymous group 1 volleys. Exp Brain Res. 19; 7(1): Ashby P, Zilm D. Relationship between EPSP shape and cross-correlation profile explored by computer simulation for studies on human motoneurons. Exp Brain Res. 19;7(1): Heckmann CJ, Gorassini MA, Bennett DJ. Persistent inward currents in motoneuron dendrites: implications for motor output. Muscle Nerve. 5;31(): Kandel E, Siegelbaum S. Synaptic integration. In: Kandel E, Schwartz J, Jessell T, eds. Principles of Neural Science. Vol. th ed. New York, NY: McGraw-Hill; : Vucic S, Kiernan MC. Cortical excitability testing distinguishes Kennedy s disease from amyotrophic lateral sclerosis. Clin Neurophysiol. ;119(5): Zhang H, Robinson N, Wu C, Wang W, Harrington MA. Electrophysiological properties of motor neurons in a mouse model of severe spinal muscular atrophy: in vitro versus in vivo development. PLoS One. 1;5(7):e doi: /journal.pone Kanai K, Kuwabara S, Misawa S, et al. Altered axonal excitability properties in amyotrophic lateral sclerosis: impaired potassium channel function related to disease stage. Brain. 6;19(pt ): Lee RH, Heckman CJ. Essential role of a fast persistent inward current in action potential initiation and control of rhythmic firing. J Neurophysiol. 1;5(1): Hess CW, Mills KR, Murray NM. Magnetic stimulation of the human brain: facilitation of motor responses by voluntary contraction of ipsilateral and contralateral muscles with additional observations on an amputee. Neurosci Lett. 196; 71(): Araki S, Hayashi M, Tamagawa K, et al. Neuropathological analysis in spinal muscular atrophy type II. Acta Neuropathol. 3;16(5):1-.. Weber M, Eisen A, Stewart H, Hirota N. The split hand in ALS has a cortical basis. J Neurol Sci. ;1(1-):66-7. ARCH NEUROL / VOL 69 (NO. ), APR 1 73
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