A712(18)- Test slide, Breast cancer tissues with corresponding normal tissues

Transcription

1 A712(18)- Test slide, Breast cancer tissues with corresponding normal tissues (formalin fixed) For research use only Specifications: No. of cases: 12 Tissue type: Breast cancer tissues with corresponding normal tissues No. of spots: 1 spot from each cancer case (12 spots) 12 non-neoplastic spots (12 spots) Total spots: 24 Corresponding normal tissues with cancers: Yes Diameter: 1. 0 mm Documents: Product specification: layout, summary of tissue spots H&E stained images Detailed pathological information Layout: P T

2 A712(18)- Test slide, Breast cancer tissues with corresponding normal tissues Summary of tissue spots (formalin fixed) For research use only P T

3 A712(18)- Test slide, Breast cancer tissues with corresponding normal tissues (formalin fixed) For research use only QC sheet_lot# Hematoxylin and Eosin staining A1 A2 A3 A4 A5 B1 B2 B3 B4 B5 C1 C2 C3 C4 C5 D1 D2 D3 D4 D5 E1 E2 F1 F2 P T

4 1 A 1 f 49 Breast, right, total mastectomy with SLNB: 1. 1) Size: 2.8x1.9cm 2) Black's nuclear grade: 1 (poorly differentiated) 3) Modified Bloom-Richardson's histological grade: III 4) Intraductal component: 15% 5) Resection margins: Negative 6) Vascular invasion: Present 7) Perineural invasion: Absent 8) Microcalcification: Present in invasive cancer, in associated with benign epithelium, in stroma 9) Immunohistochemistry: ER(+), PR(+), C erb B2 (weak +:2+/3+) 2.Nipple and areola: Free of tumor. 3.Overlying skin: Free of tumor. 4.Underlying fascia: Free of tumor. 5.Lymph node, sentinel(0/4), axillary(0/3), regional(0/2):(0/9): Free of tumor. 2 B 1 f 49 non-neoplastic non-neoplastic from A1 3 A 2 f 66 Breast, left, modified radical mastectomy: 1.Status incisional biopsy with residual invasive ductal 1) Size of the residual tumor: 2x1.8cm 2) Site: LO quadrant 3) Black's nuclear grade: I (poorly differentiated) 4) Modified Bloom-Richardson's histological grade: III 5) Intraductal component: 10% 6) Vascular invasion: Present 7) Perineural invasion: Absent 8) Microcalcification: Present, In invasive cancer, In DCIS, In association with benign epithelium 9) Immunohistochemistry: ER(-), PR(+), C erb B2(-: 0/3+) 2.Nipple and areola: Free of tumor 3.Lymph node, sentinel(2/2), axillary(2/24):(4/26):tumor metastasis in 4 out of 26 nodes 4 B 2 f 66 non-neoplastic non-neoplastic from A2

5 5 A 3 f 45 Breast, left, modified radical mastectomy: 1. 1) Size: 3x2cm 2) Black's nuclear grade: I (poorly differentiated) 3) Modified Bloom-Richardson's histological grade: III 4) EIC:present 5) Resection margins: Negative 6) Vascular invasion: Present 7) Perineural invasion: Absent 8) Microcalcification: Present in invasive cancer and ductal in situ 9) Immunohistochemistry: ER(focal +), PR(-), C erb B2(+:3+/3+) 10)Back ground ductal in situ 1 Nuclear grade: high 2 Histologic grade: high 3 Necrosis: Present (comedo) 4 Architecture type: Cribriform, solid 5 Extent of ductal in situ: about 8x5cm 6 Calcification: Present in ductal in situ 7 Microinvasion: Present 2.Nipple and areola: Free of tumor. 3.Overlying skin: Free of tumor. 4.Underlying fascia: Free of tumor. 5.Lymph node, axillary(2/31):tumor metastasis in 2 out of 31 nodes (with pericapslar tumor spread: largest size of tumor deposit: 3.3x2.5cm) 6 B 3 f 45 non-neoplastic non-neoplastic from A3

6 7 A 4 f 58 Breast, left, modified radical mastectomy: 1. 1) Size: 1.5x1.3cm and 0.5x0.3cm(each separated by 1.5cm) 2) Black's nuclear grade: 1(poorly differentiated) 3) Modified Bloom-Richardson's histological grade: III 4) EIC: Present 5) Vascular invasion: Present 6) Perineural invasion: Absent 7) Microcalcification: Absent 8) Immunohistochemistry: ER(-), PR(-), C erb B2(-: 0/3+) 9) Ductal in situ in the background 1. Nuclear grade: high 2. Histological grade: intermediate 3. Necrosis: absent 4. Architectural types: cribriform, solid 5. Extent of DCIS: 4x3cm 6. Calcification: absent 10) Associated benign changes: Fibrocystic change with florid ductal epithelial hyperplasia. 2.Nipple and areola: Free of tumor. 3.Overlying skin: Free of tumor. 4.Underlying fascia: Free of tumor. 5.Lymph node, axillary(2/35): Tumor metastasis in 2 out of 35 nodes (without pericapsular tumor spread)(size of the largest metastatic tumor: 1.5x1.0mm) 8 B 4 f 58 non-neoplastic non-neoplastic from A4

7 9 A 5 f 50 non-neoplastic Breast, ringht, skin sparing total mastectomy: 1. Size: 3x3x2cm 2. Black's nuclear grade: 1 (poorly differentiated) 4. EIC: Present 6. Vascular invasion: Absent 8. Microcalcification: Present in association with benign epithelium Overlying skin: Free of tumor. Underlying fascia: Free of tumor. Lymph node, axillary(0/3): Free of tumor. Breast, labelled as "subareolar subcutaneous tissue", excision: Free of tumor. Result of immunohistochemical stains: ER(+), PR(+), C erb B2(weak +: 2+/3+) 10 B 5 f 50 non-neoplastic non-neoplastic from A5 11 C 1 f 69 Breast, right, partial mastectomy: 1. Size: 2.2x1.3cm 2. Black's nuclear grade: 1 (poorly differentiated) 3. Modified Bloom-Richardson's histological grade:iii 4. Intraductal component: 10% 6. Vascular invasion: Absent 8. Microcalcification: Present in invasive cancer, in ductal in situ 9. ER(+), PR(+), C erb B2(weak+:2+/3+) Underlying fascia: Free of tumor. Lymph node, sentinel(0/2): Free of tumor. 12 D 1 f 69 non-neoplastic non-neoplastic from C1

8 13 C 2 f 58 Breast, right, modified radical mastectomy: 1. Size: 2.2x1.3cm 2. Black's nuclear grade: I (poorly differentiated) 4. EIC: positive 6. Vascular invasion: Absent 8. Microcalcification: Present in invasive cancer, in associated with benign epithelium, in stroma 9. Immunohistochemistry: ER(-), PR(-), C erb B2(-:0+/3+) 10. Showing histologic changes of adjuvant chemotherapy 11. Associated benign changes: Fibroadenoma. Nipple and areola: Free of tumor. Overlying skin: Free of tumor. Underlying fascia: Free of tumor. Lymph node, axillary(0/7): Free of tumor. 14 D 2 f 58 non-neoplastic non-neoplastic from C2

9 15 C 3 f 59 Breast, left, total mastectomy: 1. Size: 2.3x1.8cm 2. Black's nuclear grade: I (poorly differentiated) 4. EIC: positive 6. Vascular invasion: Absent 7. Perineural invasion: Present 8. Microcalcification: Present in invasive cancer, in ductal in situ, in associated with benign epithelium, in stroma 9. ER(-), PR(-), C erb B2(+:3+/3+) Nipple and areola: Paget's disease. Overlying skin: Free of tumor. Underlying fascia: Free of tumor. Lymph node, sentinel(0/4):free of tumor 16 D 3 f 59 non-neoplastic non-neoplastic from C3

10 17 C 4 f 46 Breast, left, partial mastectomy: 1. Size: 2.2x1.7cm 2. Black's nuclear grade: I (poorly differentiated) 4. EIC: Present Distance to closest (relevant) margin: 2mm Relevant margin is superior 6. Vascular invasion: Present 8. Microcalcification: Present in invasive cancer, in ductal in situ, in associated with benign epithelium, in stroma 9. Immunohistochemistry: ER(-), PR(+), C erb B2(-:0/3+) 10. Ductal in situ in the background 1) Nuclear grade: High 2) Histological grade: High 3) Necrosis: Present (Comedo) 4) Architectural types: Solid 5) Extent of DCIS: 9x6cm 6) Microinvasion: Absent (multifocal) Lymph node, sentinel(0/2): Free of tumor. 18 D 4 f 46 non-neoplastic non-neoplastic from C4

11 19 C 5 f D 5 f 39 non-neoplastic non-neoplastic from C5 Breast, left, partial mastectomy: mixed with mucinous 1. Size: 2.2x1.8cm 2. Black's nuclear grade: I (poorly differentiated) 4. Intraductal component: No 6. Vascular invasion: Absent 8. Microcalcification: Present in invasive cancer, in associated with benign epithelium 9. Immunohistochemistry: ER(+), PR(+), C erb B2(-:0/3+) Note: About 60-70% of the tumor consists of mucinous and this case cannot be categories as pure mucinous. E 1 f 39 Breast, left(upper outer), partial mastectomy: mixed with mucinous 1. Size: 2x1cm 2. Black's nuclear grade: II (moderately differentiated) 4. Intraductal component: No 6. Vascular invasion: Present 8. Microcalcification: Absent 9. Immunohistochemistry: ER(+), PR(+), C erb B2(-:1+/3+), Synaptophysin (focal +), chromogranin(-), NSE(-) Lymph node, sentinel (1/1), axillary (4/18): (5/19): Tumor metastasis in 5 out of 19 nodes. (size of the largest metastatic tumor: 1.8x1.5 cm) with perinodal tumor spread Note: About 40-50% of the tumor consists of mucinous. 22 F 1 f 39 non-neoplastic non-neoplastic from E1

12 23 E 2 f 46 Breast, left, partial mastectomy: 1. Size: 1.4x1.3cm 2. Black's nuclear grade: 1 (poorly differentiated) 4. Intraductal component: < 5% 5. With areas of invasive micropapillary 6. Vascular invasion: Present 8. Microcalcification: Absent 9. Immunohistochemistry: ER(+), PR(+), C erb B2(-:0/3+ ) Lymph node, sentinel (1/4), axillary (0/4): (1/8): Tumor metastasis in one out of 8 nodes with focal perinodal tumor spread. (size of the metastatic tumor: 2x1mm) 24 F 2 f 46 non-neoplastic non-neoplastic from F1