JEUNESSE Inc. Institute of Longevity Medicine 11/5/99. PRELIMINARY REPORT (additional test results to follow) Transfer Factor Testing
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1 Instituto Longevidad JEUNESSE Inc. Institute of Longevity Medicine 11/5/99 PRELIMINARY REPORT (additional test results to follow) Transfer Factor Testing Background and rationale: Cancer is the second leading cause of death in the US. The rate of nearly every type of cancer is on the rise. Despite billions of dollars in research, the mortality rate has remained virtually unchanged since the 1960's using conventional treatments such as radiation, surgery, and chemotherapy. Thus, there is considerable interest in using immune modulation as an adjuvant for cancer therapy. Natural killer cells are particularly important in destroying cancer cells. Purpose of study: To determine the in vitro anti-cancer effects of Transfer FactorÔ and Transfer Factor PlusÔ using PBMC isolated from human volunteers and assaying the increased ability of the NK cell population to kill K562 erythroleukemic cells. Methods: 1) PBMC killing of K562 (erythroleukemic) cells Results: 2) Transfer Factor increased NK-cell killing by 103% and Transfer Factor PlusÔ by 248% Conclusions: Transfer FactorÔ and Transfer Factor PlusÔ induced immune-based lysis of K562 cells at a level unprecedented in the director's experience or in the known medical literature. Since NK cell function is so crucial in killing cancer cells, these products are ideal candidates for cancer adjuvant therapy. In addition, NK cells form a first line of defense against infections from viruses and certain other microorganisms. Killing assays against K562 cells have also been correlated with increased activity against chronic and acute infections as well. Darryl See, M.D. Director, Bioassay Laboratory Institute of Longevity Medicine Huntington Beach, CA Transfer Factor Study (4Life) N.K. Assay, October 27, 1999 Sample Name #of Live K562 Cells #of Dead K562 Cells Mean of Dead cells % Increase In Killing Baseline - a Baseline - b Positive (PBMC) - a Positive (PBMC) - b Negative (Flour) - a Negative (Flour) - b Transfer Factor - a Transfer Factor - b %
2 Transfer Factor Plus Study (4Life) N.K. Assay November 4,1999 Sample None #of Live K562 Cells #of Dead K562 Cells Baseline - a Mean of Dead cells Baseline - b Positive (PBMC) - a Positive (PBMC) - b Negative (Flour) - a Negative (Flow) - b Transfer Factor Plus(200 m l) - a % Increase In Killing Transfer Factor Plus(200 m l) - b % Transfer Factor Plus(20 m l) - a Transfer Factor Plus(20 m l ) - b % Transfer Factor and Transfer Factor Plus Study (4Life) Toxicity Assay November 9, 1999 Sample Transfer Factor (2 mg) Transfer Factor (200 m g) Transfer Factor Plus (2 mg) Transfer Factor Plus (200 m g) Result Nutraceutical Induced Increase in Natural Killer Cell (NK) Activity Purpose of Study: To determine the in vitro Natural Killer Cell (NK) activation effects of 4 Life Transfer Factor and Transfer Factor Plus using peripheral blood mononuclear cells (PBMC) isolated from human volunteers and assaying the increased ability of the NK cell population to kill K562 erthroleukemic cells. Conclusions: 4Life Transfer Factor and Transfer Factor Plus induced immune-based lysis (breakdown) of K562 cells at a level unprecedented in the director's experience or in any known medical literature, Since NK cell function is so crucial in killing dangerous Cells, these products are ideal candidates for human and animal immune support. Nutraceutical Tested Percent (%) Rise in NK Cell Activity 4Life Transfer Factor Plus 248% 4Life Transfer Factor 103% IP6 (highest of group Previously tested) 49% Plant Polysaccharide Formula 48% Echinacea 47% Shitake Mushroom 42% Cordecep Formula 28%
3 Bovine Colostrum 23% Phytonutrient Formula with Garlic 21% Endocrine System Formula 16% Concentrate (acemannan) 15% Noni (Morinda citrafolia) 15% Some of these products reflect an average of different brands. Baseline activity is the measure of the unassisted ability of white blood cells to kill K562 leukemia (cancer) cells. Natural Killer Cells are the body's first line of defense against cancers and viruses. The increase in the ability of white blood cells to kill K562 cancer cells is directly related to the increase in Natural Killer Cell activity. This study does not claim nor should it be interpreted to cure, prevent or mitigate any serious disease. The results have been reported as they were received. Seek advise from your health professional before beginning any new regimen Fuente: Información con fines exclusivamente divulgativos Para mayor información sobre los Factores de Transferencia de 4Life contacte a la persona que le suministró este material
4 Jeunesse Inc. Institute of Longevity Medicine Darryl See, M.D. Director, Bioassay Laboratory Preliminary Report (additional test results to follow) Transfer Factor Testing Background and rationale: 1) Cancer is the second leading cause of death in the US. The rate of nearly every type of cancer is on the rise. Despite billions of dollars in research, the mortality rate has remained nearly unchanged since the 1960's using conventional treatments such as radiation, surgery and chemotherapy. Thus, there is considerable interest in using immune modulation as an adjuvant for cancer therapy. Natural killer cells are particularly important in destroying cancer cells. Purpose of study: 1) To determine the in vitro anti-cancer effects of Transfer Factor and Transfer Factor Plus using PBMC isolated from human volunteers and assaying the increased ability of the NK cell population to kill K562 erythroleukemic cells. Methods: 1) PBMC killing of K562 (erythroleukemic) cells Results: 1) Transfer Factor increased NK-cell killing by 103% Transfer Factor Plus increased NK-cell killing by 248% Conclusions: Transfer Factor and Transfer Factor Plus induced immune-based lysis of K562 cells at a level unprecedented in the director's experience or in the known medical literature. Since NK cell function is so crucial in killing cancer cells, these products are ideal candidates for cancer adjuvant therapy. In addition, NK cells form a first line of defense against infections from viruses and certain other microorganisms. Killing assays against K562 cells have also been correlated with increased activity against chronic and acute infections as well. Darryl See, M.D. Director, Bioassay Laboratory Institute of Longevity Medicine
5 Nutraceutical induced increase in Natural Killer cell activity Nutriceutical % Rise in Natural Killer cell activity Transfer Factor Plus 248 % Transfer Factor 103 % IP6 (highest of group previously tested) 49 % Plant Polysaccharide Formula 48 % Echinacea 43 % Shiitake Mushroom 42 % Cordeceps Formula 28 % Bovine Colostrum 23 % Phytonutrient Formula 21 % Endocrine System Formula 16 % Aloe Vera Concentrate (acemannan) 15 % Noni (Morinda Citrafolia) 15 % Note: Over the years, Jeunesse Inc. has tested 196 nutraceuticals. 152 of these products increased effectiveness from 0 to 10%. Only 44 products did better than 10%. 40% of these products were too toxic to use without caution. Until now, IP6 showed the highest increase at 49%. Transfer Factor has been shown to be the most powerful product on the market, with a toxicity level of ZERO. Transfer Factor Study with 20 Cancer Patients Darryl See, M.D. conducted the following studies. Twenty patients, 12 men and 8 women, were selected for this in vivo study. The average age was The twenty individuals were each level 3 or level 4 cancer patients. Each patient was basically sent home by his or her oncologist to die. The average life expectancy was 3.7 months. The protocol was to place each patient on 9 capsules per day of Transfer Factor Plus. The patients were given a number of other general nutrients*. After eight months, 16 of these individuals were still living and were either in remission, improving or stabilized. The baseline for natural killer cell function was 6.4. Within 4 weeks the average NK Cell function was increased to 25.7 and in 6 months it increased to This represented a 400% increase in NK Cell function. This is an ongoing study. This study has been submitted to a peer reviewed publication. *Antioxidants, Digestive Enzymes, Probiotics and multi-vitamins.
6 The following study was conducted at the Institute of Longevity. The purpose of the study was to examine the synergistic effects of the components of Transfer Factor Plus. Each component was tested separately and then tested as a whole unit. Together the proprietary blend increased NK Cell functions more than the sum total of all of the ingredients tested separately. This study indicates that the intelligence contained in Transfer Factor has an enhancing effect on other nutrients. In vitro study: Ingredient 10 GM /ml NK Function PBMC (Control) 25.6% Zinc 26.8% NS Proprietary Blend 59.9% <0.02 (Mushrooms etc) Zinc +Prop. Blend 95.4 <0.01 Transfer Factor alone 128.5% <0.01 Complete Prod. (Transfer Factor Plus ) 273.6% <0.01 The sum of the individual product is less than the Complete Product (Transfer Factor Plus ). There is synergy in having a combination of Transfer Factor Plus. The information has not been evaluated by the FDA or by 4Life Research. We do not claim that 4Life products directly prevent, or cure any disease. We do not suggest that anyone should replace traditional medical treatment with our products. 4Life products are promoted for their ability to enhance the effectiveness of our natural immune system functions. On this web site you will be exposed to many testimonies and nutritional ideas. We encourage you to use common sense and prudence in evaluating testimonies. Testimonies can be based on "placebo effects," which basically means that you can experience temporary results just because you really believe you will. Research can be complicated to translate into real life situations. Even medical doctors may disagree on certain subjects. It is our desire to expose you to many different ideas for your consideration. Our focus is on supporting the immune system, which can assist your body in the healing process. The testimonies and information on this web site only represent the opinions of the individuals involved. We encourage you to use common sense in your expectations in consuming any nutritional product. All material provided in this site is provided for educational purposes only. The information provided may not be relied upon for diagnosis or treatment of any disease or medical condition. Seek advice from your health care professional regarding the applicability of any information, opinion or recommendation for diagnosis or treatment of any symptoms or medical condition. Statements about products and health conditions have not been evaluated by the U.S. Food & Drug Administration. Products of 4Life Research are not intended to diagnose, treat, cure, or prevent any disease.
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