BIO 364: Laboratory Techniques in Cancer Biology Syllabus for Fall 2017
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1 BIO 364: Laboratory Techniques in Cancer Biology Syllabus for Fall 2017 Course Description This course will introduce contemporary concepts of cancer initiation, progression, metastasis and therapy. The lectures and recitations will include discussions of appropriate review articles, textbook readings and research articles. In the laboratory, students will be introduced to and recapitulate key techniques used in the selected research articles. This course will require significant work on computers outside of class time (more than 3 hours per week). This course has an associated fee. Please see for more information. Course Directors: Daniel Moloney, PhD Telephone Office Office Hrs Life Sciences, Room 092 TBA or by appointment or Daniel.Moloney@stonybrook.edu Jennie Williams, PhD HSC T TBA or by appointment or Jennie.Williams@stonybrookmedicine.edu Prerequisites: C or higher in BIO 205 or BIO 207 Pre- or Corequisites: BIO 314 (Cancer Biology), grade of C or higher if used as a prereq. Course Website: access through blackboard.stonybrook.edu Course Times & Locations Lectures: Recitation & Lab sections: Mondays, 12:00 12:53 pm in Humanities Room 1023 (1 st floor) Sec.01: Tuesdays, 12:00 3:50 pm in Life Sciences Room 008 (basement) Sec.02: Wednesdays, 12:00 3:50 pm in Life Sciences Room 008 (basement) Class Resources Blackboard: You are expected to access the BIO 364 Blackboard site on a regular and frequent basis to view course announcements, to obtain documents and handouts, and to complete homework and assignments. Your Stony Brook University account may also be used for important course communications. Textbook (required): Robert Weinberg, The Biology of Cancer, 2 nd edition, Garland Science Publishing Required Attendance: Attendance/Participation in all Lectures, Laboratory and Recitation sections is mandatory
2 Course Learning Objectives: 1. Students will be able to summarize the prevailing theories of cancer development and treatment. 2. Students will differentiate between interventions that prevent cancer and interventions that treat cancer. 3. Students will be able to explain at least one type of experiment used in cancer research. 4. Students will be able to read and summarize a research paper in cancer biology. 5. Students will speak directly with a cancer research scientist about a topic of interest to the student. 6. Students will be able to demonstrate knowledge of the following laboratory techniques: SDS PAGE Immunofluorescence microarray analysis Western Blotting Flow Cytometry Proliferative assay (i.e. MTT) ELISA Immunoprecipitation PCR (including RT-PCR and qpcr) Class Protocols Please remember that the BIO 364 scientists and physicians will share their knowledge and enthusiasm with you. Please give them your respect and courtesy by being attentive and on-time for class. Cell phones must be turned off during lectures, recitations and laboratory sessions. Laptop computers may be used to take notes during lectures. Internet surfing, ing, texting, or any other non-courserelated activities during lectures, recitations or labs are not permitted and will result in loss of attendance/participation points for each occurrence. Attendance and Make-up Policy Attendance and participation in all lectures, recitations and laboratory sessions is mandatory. Late submission of homework/assignments will not be accepted. Make-up recitations or lab sessions will not be available. Attendance at all exams is mandatory. Makeup exams will be provided only for legitimate and documented absences and at the discretion of the instructors. In the case of verifiable illness, family emergency, or University-sanctioned religious holiday (arrangements must be made in advance), the excuse must be documented on official letterhead and will be verified by the instructors. Absences for participation in University-sanctioned events must be arranged at the beginning of the semester. For student responsibilities regarding absences due to University-sanctioned events see: n_univsponsered_activities.php Appeal Process: After assignments are returned or exam scores provided, you may appeal the grading of specific sections or questions. You must provide a short, coherent, neatly written explanation of your reasons for requesting regrading. The appeal must be received no later than two business days after the exam score is posted or the assignment is returned. All appeals turned in at a later time will be declined. If your appeal is justified in the judgment of the faculty, a score correction will be applied. Americans with Disabilities Act: If you have a physical, psychological, medical or learning disability that may impact your course work, please contact Disability Support Services, ECC (Educational Communications Center) Building, room128, (631) They will determine with you what accommodations, if any, are necessary and appropriate. All information and documentation is confidential.
3 Academic Integrity: Each student must pursue his or her academic goals honestly and be personally accountable for all submitted work. Representing another person's work as your own is always wrong. Faculty are required to report any suspected instances of academic dishonesty to the Academic Judiciary. Faculty in the Health Sciences Center (School of Health Technology & Management, Nursing, Social Welfare, Dental Medicine) and School of Medicine are required to follow their school-specific procedures. For more comprehensive information on academic integrity, including categories of academic dishonesty, please refer to the academic judiciary website at Critical Incident Management: Stony Brook University expects students to respect the rights, privileges, and property of other people. Faculty are required to report to the Office of Judicial Affairs any disruptive behavior that interrupts their ability to teach, compromises the safety of the learning environment, or inhibits students' ability to learn. Faculty in the HSC Schools and the School of Medicine are required to follow their school-specific procedures. Grading/Evaluation will include the following: category details Points (% of total) Attendance, Participation, Homework/Assignments Lecture/recitation/lab attendance & participation, plus in-class and Blackboard assignments 70 pts (11.7%) Quizzes Journal article/cell culture quizzes 30 pts (5%) Lab Report Individual written report on lab assays 100 pts (16.7%) Group project Exams individual written summary and Team presentation Midterm exam (150 pts) and Final exam (150 pts; cumulative) 100 pts (16.7%) 300 pts (50%) Total = 600 pts (100%) Grading Scale will be calculated as follows after all scores are tallied (an adjustment or curve may be applied if deemed appropriate by the instructors): Letter Grade: A/A- B+/B/B- C+/C D F Percentage: % 89-80% 79-70% 69-60% 59% or less
4 Lecture Series: Date Session # Speaker Topic August 28 1 Jennie Williams, PhD Cancer Initiation/Nature of Cancer The Biology and Genetics of Cells and Organisms Evolution of Tumor Cells Cancer Stem Cells Hereditary vs Sporadic Cancer Tumor Viruses Sept 4 No Class Labor Day Break Sept 11 2 Dan Moloney, PhD Pathology: Defining a Neoplasm Molecular Techniques (part 1) Histopathology Cytology Sept 18 3 Dan Moloney, PhD Pathology: Defining a Neoplasm Molecular Techniques (part 2) Histopathology Cytology Sept 25 4 Amr Ghaleb, PhD Tumor Genetics DNA Damage and DNA repair Oncogenes Tumor Suppressor Genes Oct 2 5 Jingfang Ju, PhD Signaling Pathways/Cascades Involved in Cancer Progression Multistep Tumorigenesis Cancer Research In vitro/in vivo RNA and mirnas Oct 9 6 Agnieszka Bialkowska, PhD Genetic Epidemiology of Cancer by Site Colorectal Cancer Oct 16 Midterm Exam Held during class time in our classroom Oct 23 7 Dan Moloney, PhD Overview of 2 nd part of the lecture series Oct 30 8 Grace Gathungu, MD Microbiomes and Cancer Nov 6 9 Michael Hayman, PhD Signaling Pathways/Cascades Involved in Cancer Progression Growth Factor Receptors and Cancer Oncogenes as Targets for Therapy Targeting HDAC Activity in Cancer
5 Nov Daniel Irizarry Mechanisms of cancer gene control using CRISPR-CAS9 Genomic approaches to cancer research Nov Ashley Snider, PhD Inflammatory Bowel Disease and Colon Cancer Nov Paula Denoya, MD Clinical Aspects of Cancer Cancer Screening, Diagnosis and Treatment Cancer Research (Clinical) Clinical Trials Dec 4 13 Zuzana Zachar, PhD From Benchtop to Pharmacy Shelf Drug Conception Formulation Trials Production Dec 14 (Thursday) Final Exam Time: 5:30 8:00 pm in our classroom
6 Laboratory & Recitation Series: Note: Attendance in the laboratory and recitation sessions is mandatory. Any student with two or more absences from these sessions will automatically fail the course. Dates (Tues, Wed) Session # Planned Activity/Description Aug 29, 30 1 General Safety/use of micropipettes and other equipment ELISA/Epidemiology ATCC resource Sept 5, 6 No class Labor Day Break Sept 12, 13 2 Laboratory Safety Training and Working with Biohazardous Materials MTT/XTT Assay/IC 50 determination RNA extraction & rtpcr Cell Culture Techniques Sept 19, 20 3 Agarose Gel electrophoresis and rtpcr analysis Introduction to Histochemistry & Microscopy Cell Culture week 1 Sept 26, 27 4 Immunofluorescence part 1 Flow Cytometry/Cell cycle analysis Cell Culture week 2 Oct 3, 4 5 Immunofluorescence part 2 (results & analysis) Cell Lysis and Protein Assay (Bradford) Cell Culture week 3 Primary Research Article Oct 10, 11 6 Immunoblot part 1 (SDS-PAGE and Electrotransfer) Cell Culture week 4 Quiz on Primary Research Article Oct 17, 18 7 Immunoblot part 2 (Immunodetection) Cell Culture week 5 Oct 24, 25 8 ELISA Cell Culture week 6 DLAR Tour Oct 31, Nov 1 9 Projects preparation Nov 7, 8 10 Projects Nov 14, Projects Nov 21, 22 No class Thanksgiving Break Nov 28, Projects Dec 5, 6 13 Team Project Presentations
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