Poster#8. December 7 th 2017

Transcription

1 Poster#8 Advanced Development and Validation of 3D Spheroid Culture of Primary Cancer Cells using Nano3D Technology This work is supported by the NCI IMAT Award R33 CA26949 Assist. Prof. Timothy Spicer- Discovery Biology and HTS Tel: (561) December 7 th 217

2 Screening in 2D formats has left us wanting for more relevant and physiologically appropriate assays-hanging drop and other low throughput assays exist but aren t effective to rapidly translate cost effective strategies to HTS Pancreatic cancer remains a leading cause of cancer deaths, with 5-year survival rate less than 5%. Genetic alterations are associated with pancreatic cancer, including oncogene (KRAS), tumor suppressors (TP53, CDKN2A, SMAD4, ARID1A and MLL3 ). We received the following cell lines: Name Tissue Mutations hm1-caf a resected metastatic lung lesion, same as hm1 Kras wt (fibroblast, SV4 immortalized) hm1 a resected metastatic lung lesion Kras G12D, p53 R175H (epithelial-like) ht1-caf resected primary tumors, same as ht1 Kras wt (fibroblast, SV4 immortalized) ht1 resected primary tumors Kras G12V, P53 loss, SMAD4 loss, CDKN2A hom del ((epithelial-like) hm1-caf/hm1, ht1-caf/ht1 are CAF/cancer pairs derived from the same tissue. TSRI 217. All rights reserved.

3 384 well format 1536 well format Incubation hours Detach cells Figure 1: Greiner cell repellent plates readily form spheroids using cells combined with Nanoshuttle. This can be done on the bottom side of the plates using a magnetic driver. Note that the shape of the bottom of the wells is compatible with automated confocal microscopy. TSRI 217. All rights reserved.

4 A B Condition No cells Cells-Vehicle Cells-Cytotoxic compound Outcome No ATP produced no signal More ATP production High signal Less ATP production Low signal The assay determines the number of viable cells based on the amount of ATP present. The amount of ATP is detected via CellTiter-Glo reagent involving enzymatic reactions which generate a stable luminescent signal. The amount of ATP is proportional to the number of viable cells in culture. TSRI 217. All rights reserved.

5 2D HTS 4X objective 2X objective 3D HTS Z stack Confocal Image RLU LogIC5 IC5 2D: ht1-2d ~ ~ e e-8 ~ ~ ~ 2.464e e-8 RLU D: ht1-2d 75 6 LogIC5 IC ~ ~ e-6 ~ ~.1431 ~ ~ 1.53 Cells in 2D monolayer and in 3D spheroid/organoid format demonstrated different responses to therapeutic compounds. Drug resistance was observed in 3D model. Complete optimization of all 4 cell lines in 3D format followed by drug testing and HTS TSRI 217. All rights reserved.

6 EC1: Medium + DMSO 125 cells per well %CV S/N Z Z' n3d/1536: ht1 CRC RLU 15 1 EC1: cells + DMSO LogIC5 IC ~ ~ ~ 1.986e e-7 ~ ~ 173 ~ ~ %CV S/N Z Z' e-7 Condition: 125 ht1 cells per well were seed in 1536 well plate (5uL), with 4hr printing time and read at 4 days post seeding These conditions are robust- passed Z. CRC of 5 controls in 1536 wpf is consistent with that in 384 wpf. TSRI 217. All rights reserved.

7 Hit Cutoff Run Statistics (n = 6 plates) 329 compounds tested at 2.6 µm nominal concentration in duplicate Z =.72±.5 S:B = ±13.41 Hit Cutoff (Interval cut-off) = 38.91% Hits = 21 (.64%) High control is Medium only + DMSO; Low control is Cells + DMSO This assay showed excellent Z values. Doxorubicin IC 5 matched expected. Applying interval-based cutoff, 21 hits were identified in this screen. RLU n3d/1536: ht1 CRC LogIC5 IC e e-6 ~ ~ ~ 7.366e e-7 TSRI 217. All rights reserved.

8 % Inhibition D Log[Disulfiram] ht1-3d ht1-caf-3d hm1-3d hm1-caf-3d ht1-2d ht1-caf-2d hm1-2d hm1-caf-2d E Drug 3D assay 2D assay Resistance Factor ht1 ht1-caf hm1 hm1-caf ht1 ht1-caf hm1 hm1-caf ht1 ht1-caf hm1 hm1-caf Bortezomib 41.1E E E E-9 1.3E-9 1.E E-9 8.7E Carfilzomib 55.1E E E E E-9 1.3E E-9 1.2E Romidepsin 19.E-9 1.8E-9 46.E-9 3.5E E E-1 1.6E-9 2.4E Homoharringtonine 3.34E E E E E E E-9 1.3E Trametinib 4.7E-9 1.4E E-9 2.8E-6 >5.E-6 >5.E-6 2.7E-9 >5.E-6 <.9 < <.6 % Response (3D) F 1-5 Disufliram 2.1E-7 >6.58E-6 9.9E-8 >6.58E-6 >6.58E-6 >6.58E-6 3.1E-7 2.E-7 <.3 /.3 > Approved Drugs vs. ht1 III % Response (2D) I II % Response (3D) 1-5 Approved Drugs vs. ht1-caf 5 III % Response (2D) I II % Response (3D) 1 5 Approved Drugs vs. hm1 III % Response (2D) I II %Response (3D) 1 Approved Drugs vs. hm1-caf 5-5 III %Response (2D) I II The pancreatic cancer cells and CAFs are compatible with N3D technologies. CRC of control drugs are reproducible overtime and show differential response in 3D vs 2D HTS of ~32 approved drugs in both 2D and 3D demonstrates marked difference in sensitivity TSRI 217. All rights reserved.

9 Mechanical and Automation testing Plate stability with Carousel rotation (384w and 1536w) Robotic gripper plate handing Testing of automated plate placement and removal Robotic Gripper can flawlessly place plates onto driver Magnet alignment appears correct No positioning errors induced with carousel rotation. Metal lid prevents lateral movement of plate. TSRI 217. All rights reserved.

10 RLU n3d/1536: ht1-caf CRC (Online V3.) S/N Z Z' RLU n3d/1536: ht1-caf CRC (offline) 2 S/N Z Z' LogIC5 IC e e e-8 ~ ~ ~ e-9 LogIC5 IC e e e e-9 Condition Z Conclusion before spin after spin after shaking Before spin Shaking may be necessary for 384w test, but not in After spin w; Spin is necessary to After remove bubbles, and shaking homogenize the signal. 384w 1536 w Using ht1-caf cells, 1536 drive V3. achieved similar CRC of controls as the offline test. Spinning of plates (125rpmX5min) after dispense CTG suffices in 1536w test. TSRI 217. All rights reserved.

11 This work is supported by the NCI IMAT Award R33 CA Madoux F, Tanner A, Vessels M, Willetts L, Hou S, Scampavia L, Spicer T. A 1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on Spheroids. SLAS Discovery. 217 Jan. PMID: Singhera F, Cooper E, Scampavia L, Spicer T. Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates. Talanta. 217 Sep. TSRI 217. All rights reserved.