Quality Assurance Project Plan

Transcription

1 By Kris McCaig at 1:29 pm, Sep 19, 2013 Quality Assurance Project Plan Upper Columbia River Phase 2 Sediment Study Split Sample Metals Analysis Upper Columbia River, Washington Prepared for U.S. Environmental Protection Agency Region 10 September 2013 AES10 Architect and Engineering Services Contract Contract No. 68-S Prepared by

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3 Ginna Grepo-grove Digitally signed by Ginna Grepo-grove DN: cn=ginna Grepo-grove, o=usepa, ou=oea, c=us Date: :36:45-07'00'

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5 Distribution List Name/Organization Laura Buelow EPA Task Order Project Officer Matt Wilkening EPA Task Order Project Officer Gina Grepo-Grove EPA Quality Assurance Manager/ CLP Project Officer JenniferCrawford EPAQAO EPARegionalRSCC Marilyn Gauthier CH2M HILL Project Manager Artemis Antipas CH2M HILL Quality Assurance Officer Cameron Irvine CH2M HILL Field Team Leader Tina Rice CH2M HILL Data Base Manager Address/Phone/ U.S. EPA, Hanford Project Office 309 Bradley Blvd, Suite 115 Richland, WA Phone: Fax: U.S. EPA, Idaho Office 950 W. Bannock St Boise, ID Phone: 208/ , Fax: 208/ EPA Region Sixth Avenue Seattle, WA (206) EPARegion SixthAvenue Seattle,WA98101 (206) th Ave SW, Suite 300 Portland, OR (503) Marilyn.Gauthier@ch2m.com th Ave NE, Suite 400 Bellevue, WA (425) Artemis.Antipas@CH2M.com 2485 Natomas Park Drive Suite 600 Sacramento, CA Cameron.Irvine@CH2M.com 2020 South West 4th Avenue 3rd Floor Portland, OR (503) Tina.Rice@Critigen.com Document Distribution QAPP, FSP QAPP, FSP QAPP QAPP(ecopy only) QAPP, FSP QAPP (e-copy only) QAPP (e-copy only) QAPP (e-copy only) V

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7 Contents Section Page Title and Approval Sheet... iii Distribution List... v Contents... vii Acronyms and Abbreviations... ix Introduction Project Management (EPA Group A) Project/TaskOrganization(A4) Problem Definition/Background (A5) Background Purpose Problem Definition Project Description (A6) Description of Work Tasks Project Schedule Quality Objectives and Criteria (A7) Project Quality Objectives Measurement Performance Criteria Special Training/Certification (A8) Documents and Records (A9) Data Generation and Acquisition (EPA Group B) Sampling Design (Experimental Design) (B1) Sampling Methods (B2) Sample Handling and Custody (B3) Chain-of-Custody Custody Seals Field Notebooks Corrections to Documentation Analytical Methods (B4) Quality Control (B5) Field Quality Control Procedures Laboratory Procedures Instrument/Equipment Testing, Inspection, and Maintenance (B6) Instrument/Equipment Calibration and Frequency (B7) Field Calibration Procedures Laboratory Calibration Procedures Inspection/Acceptance of Supplies and Consumables (B8) Non-direct Measurements (B9) VII

8 Contents, Continued 3.10 Data Management (B10) Assessment and Oversight (EPA Group C) Assessments and Response Actions (C1) Reports to Management (C2) Data Validation and Usability (EPA Group D) Data Review, Verification, and Validation (D1) Verification and Validation Methods (D2) Reconciliation with User Requirements (D3) Precision Accuracy Completeness (Statistical) References Figures 2-1 Project Organization 2-2 Data Flow 2-3 Phase 2 Sediment Sample Locations Tables 2-1 Data Needs and Uses 2-2 Measurement Performance Criteria Analytical Measurements for Sediment Split Samples 2-3 Analytes, MDLs, MRLs, and Ecological Screening Values for Phase 2 Sediment Samples 2-4 Phase 2 Sediment Split Sample Metals Quantities and Analytical Methods 2-5 Proposed Phase 2 Sediment Split Locations for Metals Analysis Appendices A Data Quality Objectives B Field Oversight Plan C Health and Safety Plan VIII

9 Acronyms and Abbreviations AES BLM CLP DQO FSP FTL HSP MDL MEL PM PO QA QAO QAPP QC RI RPD RSCC RSD RTL SOP SRM TAI TOPO TSU UCR EPA Architect and Engineering Services Bureau of Land Management Contract Laboratory Program Data Quality Objective Field Sampling Plan Field Team Leader Health and Safety Plan Method Detection Limit Manchester Environmental Laboratory Project Manager Project Officer Quality Assurance Quality Assurance Officer Quality Assurance Project Plan Quality Control Remedial Investigation relative percent difference Regional Sample Control Coordinator relative standard deviation Review Team Leader Standard Operating Procedure standard reference material Teck American Incorporated Task Order Project Officer Technical Support Unit Upper Columbia River U.S. Environmental Protection Agency IX

10 ACRONYMS AND ABBREVIATIONS USGS U.S. Geological Survey VIII (UCR 2013_SEDIMENT_SPLIT_QAPP_ CLEAN)

11 SEA /ES SEA 1-1 SECTION 1 1 Introduction ThisQualityAssuranceProjectPlan(QAPP)presentsthepolicies,organizations,objectives,and functionalactivities/proceduresforthephase2sedimentstudysplitsamplemetalsanalysisbeing conductedbytheu.s.environmentalprotectionagency(epa)intheuppercolumbiariver, Washingtonin2013.TheQAPPanditssupportingdocuments,foundinAppendixA(DataQuality Objectives[DQOs])havebeendevelopedtodocumentthetypeandqualityofdataneededfor environmentaldecisions.note,thesplitsamplesdescribedinthisqappwillbecollectedinthe laboratoryfollowinghomogenizationofsamplescollectedatthedesignatedlocations.therefore, thisqappdoesnotcontainanappendixdescribingthefieldproceduresforsplitsamplecollection. TheQAPPfollowsEPAguidelinescontainedinEPAGuidanceforQualityAssuranceProjectPlans (EPA,2002a),andEPARequirementsforQualityAssuranceProjectPlans(EPA,2001,reissued2006). ThecontentsoftheQAPPalsomeettheUniformFederalPolicyforQualityAssuranceProjectPlans (EPA,2005).Thedevelopment,review,approval,andimplementationoftheQAPPispartofEPA s mandatoryqualitysystem,whichrequiresallorganizationstodevelopandoperatemanagement structuresandprocessesinordertoensurethatdatausedinagencydecisionsareofthetypeand qualityneededfortheirintendeduse.thisdocumentstructurecorrelateswiththesubtitlesfoundin theepaguidelines(epa,2001,2006),consistentwithuniformfederalpolicyforqualityassurance ProjectPlans(EPA,2005.) Thisdocumentisorganizedasfollows: Section1 Introduction.Providesthepurposeandorganizationofthisreport. Section2 ProjectManagement(EPAGroupA).Providesasummaryleveldescriptionofthe projectandtaskorganization;backgroundandproblemdefinition;worktasksandproject schedule;qualityandobjectivescriteria;specialtrainingandcertifications;anddocumentsand records. Section3 DataGenerationandAcquisition(EPAGroupB).Describesthesamplingdesign; samplingmethods;samplehandlingandcustody;analyticalmethods;qualitycontrol; instrument,equipmenttesting,inspectionandmaintenance;instrument/equipmentcalibration andfrequency,inspection/acceptanceofsuppliesandconsumables;nondirectmeasurements; anddatamanagement. Section4 AssessmentandOversight(EPAGroupC).Describesassessment,oversight,and reportstomanagement. Section5 DataValidationandUsability(EPAGroupD).Introducestheconceptsofdata review,verification,andvalidation;describesverificationandvalidationmethods;andexplains reconciliationwithuserrequirements. Section6 References.Providesalistofreferencesusedinthisdocument. Inadditiontothesectionssummarizedabove,thisQAPPcontainsthefollowingappendedmaterials: AppendixA SystematicPlanning/DataQualityObjectives AppendixB FieldOversightPlan AppendixCHealthandSafetyPlan

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13 SECTION2 2 Project Management (EPA Group A) 2.1 Project/TaskOrganization(A4) ThetaskorderforthisprojectwasissuedpursuanttoEPAArchitectandEngineeringServices(AES)ContractNo. 68S70401.ThetaskorderismanagedbyCH2MHILL sprojectmanager(pm),whoworksdirectlywiththeepa TaskOrderProjectOfficer(TOPO)toaccomplishthetaskorder.ThePMmanagesthefinancial,scheduling,and technicalaspectsofthetaskorder.thekeypeopleinvolvedininterfacingwiththepmaretheepatopoandthe CH2MHILLQualityAssuranceOfficer(QAO),ReviewTeamLeader(RTL),TaskLeader,andFieldTeamLeader(FTL). NotethatallofthesamplecollectionactivitiesattheSitewillbeconductedbyTeckAmericanIncorporated,(TAI); splitsampleswillbecreatedbytai slaboratoryfollowinghomogenizationofthefieldsamples.therefore,the FTLforthisprojectwillonlyberesponsibleforfieldoversightofsamplecollectionactivities.Fieldoversight proceduresaredocumentedinthefieldoversightplan,includedasappendixb. TheprojectorganizationandlinesofauthorityforCH2MHILLstaffareillustratedonFigure21.Thedataflowis shownonfigure22.thedataforthistaskorderarelimitedtolaboratoryanalyses.figure21showstheepaand CH2MHILLtechnicalandqualityassurancepersonnel.Theorganizationalfunctionsshownareconsistentwiththe overallaes10programplan(epamanagementplansandstandardoperatingproceduresforregion10architect EngineeringServices,ContractSolicitationNo.PRR [EPA,2003aandupdates]).TheAES10Program Planprovidesadditionaldetailsfortheseorganizationalfunctions. Thefollowingadditionalorganizationalguidelinesapply: Thereviewteam(ledbytheRTL)andtheQAOwillreviewprojectplanningdocuments,dataevaluation,and deliverables.theprimaryresponsibilityforprojectqualityrestswiththepm,andindependentqualitycontrol isprovidedbythertlandqao. ThefieldteamwillimplementtheFieldOversightPlanandHealthandSafetyPlan(HSP).Thesitesafety coordinator,whoisalsotheftl,isresponsibleforadherencetothehspprocedures.theentirefieldeffortis directedbytheftl.fieldteamresponsibilitiesarefurtherdescribedinthefieldoversightplanandhsp. ThesubcontractadministratorwillprocuresubcontractsforEPA saesprojectsunderfederalacquisition Regulationsandprovidestheinterfacewithsubcontractors.Subcontractorsmaybeusedonthistaskorderfor laboratoryanalysesdependingoneparegionallaboratoryavailability. Wherequalityassuranceproblemsordeficienciesrequiringspecialactionareuncovered,thePM,RTL,and QAOwillidentifytheappropriatecorrectiveactiontobeinitiatedbytheFTL. EPARegion10(R10)adherestoanationalEPAFieldandAnalyticalServicesTeamingAdvisoryCommittee (FASTAC)strategyforprocurementofallSuperfundanalyticalservices.FASTACconsistsofEPAHeadquarters, RegionalSuperfundProgramstaff,andResearch,ScienceandTechnology(RS&T)managers.TheFieldand AnalyticalServicesTeamingAdvisoryCommittee(FASTAC)developeda DecisionTree analyticalstrategyin 1998whichhasbeenimplementedineveryEPARegion.AccordingtotheRegion10(R10)Quality ManagementPlan(EPA,2009a),analyticalservicesrequestsarefunneledthroughtheRegionalSample ControlCenter(RSCC)Coordinatorwhoselectstheanalyticalvehicleaccordingtothefollowingorder: Tier1 EPARegionalLaboratoryandEnvironmentalServicesAssistanceTeam(ESAT)Contract Tier2 NationalAnalyticalServicesContracts(ContractLaboratoryProgram[CLP]) 1 1 Information about the EPA Contract Laboratory Program may be found on the CLP Web site: SEA /ES SEA 2-1

14 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) Tier3 RegionSpecificAnalyticalServicesContracts Tier4 AnalyticalServicesInteragencyAgreements(IAGs)andFieldContracts/Subcontracts AQAPPandR10AnalyticalServicesRequestFormarerequiredfortheRSCCtobeginlaboratorycoordination. TheR10laboratoryisofferedfirstrightofrefusalbeforeproceedingtoTier2.RSCClabcoordinationoccurs afterqappdevelopment.therefore,laboratoryandanalyticalspecificsthroughouttheqappmustbe applicabletoeithertheepar10manchesterenvironmentallaboratory(mel)oralaboratorywithintheepa CLP,aslaboratoryassignmentisunknownduringtheplanningprocess.Laboratoriesarerequiredtomeetthe analyticalrequirementssetforthinthisqappformethodology,reportinglimits,qualitycontrol,anddata management.thelaboratorydataflowispresentedinfigure22. TheEPARSCCisresponsibleforbothCLPandEPAMELcoordination.TheRSCCworkswiththeEPARegional QualityAssuranceManager,theregion sclpprojectofficer(po),andtheproject spmsinresolving laboratoryandfieldqualityassurance(qa)issuesandlaboratoryscheduling.thersccprovidestheregional sampletrackingnumbers,sampletags,custodyseals,andotherclprequiredchainofcustody documentation. 2-2 SEA /ES SEA

15 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) FIGURE21 ProjectOrganizationandLinesofAuthority AnalyticalLaboratory R10MEL/CLP EPARegion10 RSCC JenniferCrawford EPARegion10 QualityAssuranceManager GinaGrepoGrove EPATaskOrder ProjectOfficer LauraBuelowand MattWilkening EPAContracting Officer EPA Contractors QualityAssuranceOfficer ProjectManager ReviewTeamLeader ArtemisAntipas MarilynGauthier FrankDillon TaskManager CameronIrvine ContractFinancial Manager LoriGilbertson ContractAdministrator LisaHill SeniorRiskAssessor FrankDillon FieldTeamLeader CameronIrvine Database Management TinaRice SEA /ES SEA 2-3

16 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) FIGURE22 DataFlow LABORATORYDATA EPARegional Laboratory EPATOPO CH2MHILL Datamanager WQX EPACLP SMOPortal EDM DataValidation (S2BVE) EPAData Validation (S4VEM) CH2MHILL QAO CH2MHILL Data Manager WQX CH2MHILL Subcontractedlab CH2MHILL QAOData Validation CH2MHILL Data Manager WQX FIELDDATA* EPARSCC CH2MHILL FieldTeam Lead CH2MHILL DataQC CH2MHILL Data Manager WQX ScribeData Manager Contracted Labs *Fielddatawillbelimitedtooversightnotes;thesplitsampleswillbecreatedandsubmittedfor analysisfollowinghomogenizationattai slaboratory. 2-4 SEA /ES SEA

17 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) Figure SEA /ES SEA

18 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) 2.2 Problem Definition/Background (A5) Background Onehundredandforty(140)sedimentlocationshavebeenidentifiedforstudyaspartofthe BaselineEcologicalRiskAssessment(BERA)thatispartoftheRemedialInvestigation/Feasibility Study(RI/FS)beingconductedbyTAIunderEPAoversightattheUCRsiteinnortheastern Washington.TAIisresponsibleforcollection,processing,andanalysisofthesedimentsamples,as detailedintheuppercolumbiariverqualityassuranceprojectplanforthephase2sedimentstudy (Phase2SedimentQAPP)preparedbyTAI. IndependentstudiesconductedtodateattheUCRsitehaveidentifiedanumberofchemicalsof potentialconcern(copcs)insedimentthatmayadverselyaffectbenthicorganisms.thesestudies donot,however,sufficientlyestablishpotentialconcentrationresponserelationships,nordothey fullyintegratemeasuresofbioavailability.asaresult,theprimarypurposeoftheoverallphase2 sedimentstudybeingconductedbytaiistoevaluateifthereareunacceptableriskstobenthic organismsassociatedwithexposuretosediment/porewatercopcs.todothis,additional sediment/porewaterchemistrydataandsynopticbenthictoxicitytestsareneeded.taidata collectioneffortswillfocusonobtaininginformationthatwillinformourunderstandingofpotential relationshipsbetweensedimentchemistryandtoxicity. ThisQAPPisfocusedonanalysisofsplitsamplesfromthePhase2sedimentsamplingeffort.Phase 2samplelocationsareshowninFigure23.Thesplitsampleswillbeobtainedatasubsetofthese locationsfollowinghomogenizationattai slaboratory.thesplitsedimentsamplesobtainedunder thisqappwillbeanalyzedfortotalchemistryonly,nobiotoxicitytestswillbeperformedusingthese splits.inaddition,nosplitsampleswillbecollectedfromtheporewatersamplesobtainedinthe fieldorinthelaboratory. Asplitsampleisasamplethathasbeenhomogenizedandequallydividedintotwoormore subsamples.thepurposeofthesplitsampleanalysisistoevaluatetheanalyticaldataprovidedby TAI ssubcontractedlaboratoriesfor:(1)comparabilityofresultsprecision(2)samplehomogeneity andsplittingefficiencyand(3)laboratoryperformance Purpose ThisQAPPpresentsthepolicies,organizations,objectives,andfunctionalactivities/proceduresfor thephase2sedimentstudysplitsamplemetalsanalysis.theqappwasdevelopedtodocument thetypeandqualityofdataneededforenvironmentaldecisionsandtodescribethemethodsfor collectingandassessingthosedataduringtheimplementationofthisstudy Problem Definition TheprimaryobjectiveofthePhase2SedimentStudySplitSampleMetalsAnalysisistocollect additionalsedimentdatathatwillinsurethereliablecharacterizationofcopcconcentrationsfor useindevelopinganunderstandingofpotentialrelationshipsbetweensedimentchemistryand toxicity.thesedimentsampleresultsmayalsobeusedtoinformthenatureandextentevaluations fortheri/fs.dataqualityobjectives(dqos)fortheseusesaredetailedinthephase2sediment StudyQAPP. 2-6 SEA /ES SEA

19 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) ThefollowingproblemstatementwasidentifiedduringtheprocessofidentifyingDQOsforthesplit samplesformetalsanalysis: SamplescollectedduringthePhase2Sedimentstudywillbeanalyzedbyalaboratory subcontractedtotai.aspartoftheoversightactivities,theepawillobtainsplitsof approximately15percentofthesedimentsamples(approximately21samples)designated forwholechemistryanalysistoassesscomparabilityofresults precision,sample homogeneityandsplittingefficiency,andlaboratoryperformance. 2.3 Project Description (A6) Description of Work Tasks TheworkactivitiescoveredunderthisQAPPincludethefollowing: AnalysisofsplitsedimentsamplesfollowingprocessingatTAIlaboratory Reportresults Project Schedule ThePhase2sedimentsamplingeffortisscheduledtobegininSeptember2013andcontinueforfive tosevenweeks.proposedsedimentsamplelocationsareshowninfigure23.sampleswillbe shippedfromthefieldtothelaboratorybytai.thelaboratorywillhomogenizethesamplesand createsplitsfromdesignatedsamplelocationsonaweeklybasis. 2.4 Quality Objectives and Criteria (A7) Project Quality Objectives ProjectspecifictechnicalsystematicplanninghasbeencarriedoutthroughtheDQOprocess planningtool(epa,2006)tomeetdecisionmakeranddatauserneedsforeachactivity.appendixa presentsthedqodecisionmakingprocessfindingsforthesplitsamples. ThedataneedsasdeterminedthroughtheDQOprocessarepresentedinTable21(locatedatthe endofthissection).thistableliststhespecificanalytes,datauses,datausers,andneededdetection levels.measurementperformancecriteriaforthesplitsamplesarelistedintable22.theselected analyticalmethodologyandassociatedlaboratoryandfieldanalyticalreportinglimitsareshownin Table23. TheneededdetectionlimitsorEcologicalScreeningValuesforPhase2SedimentSamplesandthe analyticalreportinglimits(table23)arecomparedinstep5ofthedqos(appendixa).thetarget analyticalreportinglimits(i.e.,methoddetectionlimits[mdls]andmethodreportinglimits[mrls]) areconsistentwiththeneededlimits.theselectedmethodsarestateoftheartandwhatare appropriateforthisstudy.formostanalytes,laboratoryspecificmdlsareexpectedtobebelow neededdetectionlevelslistedintable23.wheresamplespecificreportinglimitsarehigherthan neededlimits,theprojectteamwillusemdls,asneededandavailable,forprojectdecisions. Asthesplitsampledatawillbeusedtocomparethetwosets,equivalentanalyticalmethodologies anddetectionlimitrequirementsidentifiedforthelaboratoryinthephase2sedimentqappwillbe appliedtothesplitsamplingprogram.theseanalyticalmethodologiesarelistedintable22,the estimatedlaboratorymdlsandmrlsfortaidataandpotentialecologicalscreeningvaluesforeach analyteareshownintable23.table24summarizesthesplitsamplestobecollectedformetals SEA /ES SEA 2-7

20 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) metalsanalysisduringthephase2sedimentsamplecollectionevent.table25liststheproposed locationsforeachofthesamplesplits Measurement Performance Criteria TheQAobjectiveofthisplanistoidentifyproceduresandcriteriathatwillprovidedataofknown andappropriatequalityfortheneedsidentifiedinsection2.4.1.dataqualityisassessedby representativeness,comparability,accuracy,precision,andcompleteness.theseparameters,the applicableprocedures,andlevelofeffortaredescribedinthefollowingparagraphs. Theapplicablequalitycontrol(QC)procedures,quantitativetargetlimits,andlevelofeffortfor assessingdataqualityaredictatedbytheintendeduseofthedataandnatureoftheanalytical methods.analyticalparameters,analyticalmethods,applicabledetectionlevels,analyticalprecision, accuracy,andcompletenessinalignmentwithneedsidentifiedinsection2.4.1arepresentedin Table22.AnalyticalmethodsandqualitycontrolproceduresarefurtherdetailedinSection3. Reportingdetectionlevels/targetdetectionlimitslistedinTable22arelaboratoryMRLs, equivalenttomelreportinglimitsorepaclpcontractrequiredlevels. Target impliesthatfinal sampledetectionlevelsmightbehigherbecauseofsamplematrixeffects.samplereportinglimits willbeelevatedasafunctionofsamplemoisturesinceconcentrationsarereportedonadryweight basis.detectionlevelsfortheindividualsampleswillbereportedinthefinaldata.asdescribedin Section2.4.1,someofthereportinglevelsmightbehigherthanneededlimitsbecauseofmatrix effect,dilutions,preparation/digestionweight(solids)orbecausenopracticablemethodologyfor lowerdetectionisavailable.laboratoryspecificmdlsaresignificantlybelowreportinglevels. Wherereportinglimitsarehigherthanregulatorylimits,theprojectteamwilluseMDLs,asneeded, forprojectdecisions.valuesbelowthereportingareanestimateandwillbequalifiedforproper use. Followingaredefinitionsandlevelsofeffortforthedataassessmentparameters. Representativenessisameasureofhowcloselytheresultsreflecttheactualconcentrationor distributionofthechemicalcompoundsinthematrixsamples.samplingplandesign,sampling techniques,andsamplehandingprotocols(e.g.,forstorage,preservation,andtransportation)have beendevelopedandarediscussedinappendixa.theproposeddocumentationwillestablishthat protocolshavebeenfollowedandsampleidentificationandintegrityensured. Comparabilityexpressestheconfidencewithwhichonedatasetcanbecomparedtoanother.Data comparabilitywillbemaintainedusingdefinedproceduresandtheuseofconsistentmethodsand consistentunits.actualdetectionlimitswilldependonthesamplematrixandwillbereportedas definedforthespecificsamples. Accuracyisanassessmentoftheclosenessofthemeasuredvaluetothetruevalue.Forsamples, accuracyofchemicaltestresultsisassessedbyspikingsamplesandblankswithknownstandards andestablishingtheaveragerecovery.foramatrixspike,knownamountsofastandardcompound identicaltothecompoundsbeingmeasuredareaddedtothesample.aquantitativedefinitionof averagerecoveryaccuracyisgiveninsection5.3.accuracyisacombinationofrandomerror (precision)andsystematicerror(bias),introducedduringsamplingandanalyticaloperations.biasis thesystematicdistortionofameasurementprocessthatcauseserrorsinonedirection,sothatthe expectedsamplemeasurementisalwaysgreaterorlessertothesamedegreethanthesample s truevalue.theaccuracyofmeasurementdatawillbedeterminedbycalculatingtherecoveriesfrom theanalysisofstandardreferencematerialsandlaboratoryandlaboratoryfortifiedsamples(matrix 2-8 SEA /ES SEA

21 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) spikes).accuracymeasurementswillbecarriedoutwithaminimumfrequencyof1in20samples analyzed. Precisionofthedataisameasureofthedataspread,whenmorethanonemeasurementhasbeen takenonthesamesample.precisioncanbeexpressedastherelativepercentdifference;a quantitativedefinitionisgiveninsection5.3.thelevelofeffortforprecisionmeasurementswillbe aminimumof1in20samples. Completenessisameasureoftheamountofvaliddataobtainedfromtheanalyticalmeasurement systemandthecompleteimplementationofdefinedfieldprocedures.thequantitativedefinitionof completenessisgiveninsection5.3.thetargetcompletenessobjectivewillbe90percent;the actualcompletenessmightvarydependingontheintrinsicnatureofthesamplesandtheabilityto assesssamplelocationsandcollectfieldsamples.thecompletenessofthedatawillbeassessed duringqcreviews. 2.5 Special Training/Certification (A8) Allprojectstaffworkingonthesitewillbetrainedinhealthandsafetyandfollowrequirements specifiedintheproject shsp.thehspdescribesthespecializedtrainingrequiredforpersonnelon thisprojectandthedocumentationandtrackingofthistrainingisalsoincludedinthehsp. 2.6 Documents and Records (A9) ProjectsystematicplanningthroughtheDQOisdocumentedinAppendixAofthisQAPP. RequiredfielddocumentationandrecordsforfieldoversightaredescribedinAppendixB. LaboratorydocumentationwillbeprovidedinaccordancewithmethodsandQAprotocolslistedin Sections3.4and3.5ofthisQAPPandwithEPARegionalLaboratoryspecificstandardoperating procedure(sops). OverallprojectdocumentationwillbepreparedinaccordancewiththeEPARegion10AESProgram Plan(EPA,2003aandbandupdates). SEA /ES SEA 2-9

22 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) TABLE 2-1 Data Needs and Uses Matrix Analytical Suites DataUse DataUser Neededdetectionlevels(Lowest ProjectCriteria/TechnicalCriterion) a Sediment TALMetals CompareEPAsplit sampledata resultswithtai results. Chemists, Regulators DefinedbyPhase2SedimentQAPP (seetable23) TABLE 2-2 Measurement Performance Criteria Analytical Measurements for Sediment Split Samples (a) Analytical Suite Analytes Method (b) Accuracy (percent) Precision (RPD) Completeness (percent) TALMetals Aluminum,antimony,arsenic, barium,beryllium,cadmium, chromium,cobalt,copper,lead, manganese,nickel,selenium,silver, thallium,vanadium,zinc CLPorEPA 6020A (b) Calcium,iron,magnesium, potassium,sodium CLPorEPA 6010C (b) Mercury CLPorEPA 7471B (b) (a) MeasurementperformancecriteriainthistablearethesameasforTAIdatashowninTableB51.Measurement QualityObjectives,Phase2SedimentStudyQAPP(TAI2013) (b) MethodologyandQA/QCperEPARegionallaboratory(MEL)orEPACLPstandardoperatingprocedures SEA /ES SEA

23 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) TABLE23 Analytes,MDLs,MRLs,andEcologicalScreeningValuesforPhase2SedimentSamples Method Analyte ToxicityBenchmark Values a (mg/kgdw) CRQL ICPAES 6020A Aluminum NA A Antimony CRQL ICPMS 6020A Arsenic A Barium NA A Beryllium NA A Cadmium A Chromium NA A Cobalt NA A Copper NA A Lead NA A Manganese A Nickel A Selenium NA A Silver NA A Thallium NA A Vanadium NA A Zinc C Calcium NA C Iron C Magnesium NA C Potassium C Sodium B Mercury NA a BasedonchronicTECsSource:MacDonaldetal.(2000) b NondetectswillbereportedtotheMDL.ValuesbetweentheMDLandtheMRLwillbeestimated(i.e.,"J"qualified). CRQL=Contractrequiredquantitationlimit ICP AES=Inductivelycoupledplasmaatomicemissionspectroscopy ICPMS=Inductivelycoupledplasmamassspectrometry mg/kg=milligramsperkilogram dw=dryweight NA=notapplicable TEC=thresholdexposureconcentrations SEA /ES SEA 2-11

24 SECTION 2 PROJECT MANAGEMENT (EPA GROUP A) TABLE24 Phase2SedimentSplitSampleMetalsQuantitiesandAnalyticalMethods Analytes TotalMetals (aluminum,antimony,arsenic,barium, beryllium,cadmium,chromium,cobalt, copper,lead,manganese,nickel, selenium,silver,thallium,vanadium, zinc) TotalMetals (calcium,iron,magnesium,potassium, sodium) Quantity SamplePreparation QuantitativeAnalysis Protocol Procedure Protocol Procedure EPA3050B AcidDigestion EPA6020Aor ICP/MS orclp CLP EPA3050B orclp AcidDigestion EPA6010Cor CLP ICP/AES Mercury(total) 21 EPA7471B orclp Notes: AA=atomicabsorption AES=atomicemissionspectrometry EPA=USEnvironmentalProtectionAgency ICP=inductivelycoupledplasma MDL=methoddetectionlimit MRL=methodreportinglimit MS=massspectrometry AcidDigestion EPA7471Bor CLP Cold Vapor/AA Table25 ProposedPhase2SedimentSplitLocationsforMetalsAnalysis Split Group Basedon mpecq Scheduled sampling week Projected sampleshipping (fromfield) SampleLocationID* LocationPriority ProposedAnalysis 8C4 Primary TALMetals >1<4Pri 12Sep 17Sep Ref4 Primary TALMetals <1Ref 12Sep 17Sep Ref8 Primary TALMetals <1Ref 12Sep 17Sep 6B3 Primary TALMetals >1<4Pri 16Sep 23Sep 6BC2 Primary TALMetals <1Pri 16Sep 23Sep 7B5 Primary TALMetals >1<4Pri 16Sep 23Sep 5B2 Primary TALMetals >1<4Pri 23Sep 30Sep 5B5 Primary TALMetals >1<4Pri 23Sep 30Sep 5B6 Primary TALMetals <1Pri 23Sep 30Sep 5BC3 Primary TALMetals <1Pri 23Sep 30Sep 5C3 Primary TALMetals >1<4Pri 23Sep 30Sep 4B3 Primary TALMetals >4<8Pri 30Sep 7Oct 4C6 Primary TALMetals <1Pri 30Sep 7Oct 3B3 Primary TALMetals >8Pri 6Oct 14Oct 3C4 Primary TALMetals >1<4Pri 6Oct 14Oct Trib3 Primary TALMetals <1Ref 7Oct 14Oct 2B2 Primary TALMetals >1<4Pri 14Oct 21Oct 1B2 Primary TALMetals >4<8Pri 20Oct 28Oct 1B3 Primary TALMetals >1<4Pri 20Oct 28Oct 1C1 Primary TALMetals >8Pri 20Oct 28Oct 1C3 Primary TALMetals >4<8Pri 20Oct 28Oct *Ifsamplingisnotfeasibleatproposedlocation,splitwillbetakenatoneofthereservelocationsassignedtothislocation 2-12 SEA /ES SEA

25 SECTION 3 3 Data Generation and Acquisition (EPA Group B) Thissectiondescribesthesamplingdesign;samplingmethods;samplinghandlingandcustody; analyticalmethods;qualitycontrol;instrument/equipmenttesting,inspectionandmaintenance; instrument/equipmentcalibrationandfrequency,inspection/acceptanceofsuppliesand consumables;nondirectmeasurements;anddatamanagement. 3.1 Sampling Design (Experimental Design) (B1) TherationaleforandthedesignisdescribedinstepsevenoftheDQOprocessshowninAppendixA, DataQualityObjectives. 3.2 Sampling Methods (B2) TAIwillcollectandprocess(forexample,homogenizeandpresssieveto2mm)thesediment samplesinthelaboratoryusingthemethodsdetailedinthe EPA-approved Phase 2 Sediment Study QAPP.Approximately200gramsoftheprocessedsedimentwillbemadeavailabletotheEPAfor eachsplitsample.ch2mhillandepawillberesponsibleforconfirmingwhichsamplelocations requiresplitsandforpickingupandshippingthesplitsamplesfromtai slaboratorytomelortoa CLPlaboratory.Thefollowingsectionsapplytothesampledocumentationandhandling proceduresthatwillfollowprocessingattai slaboratory. 3.3 Sample Handling and Custody (B3) Asampleisphysicalevidencecollectedfromapotentialhazardouswastesite,theimmediate environment,oranothersource.becauseofthepotentialevidentiarynatureofsamples,the possessionofsamplesmustbetraceablefromthetimethesamplesarecollecteduntiltheyare introducedasevidence.inadditiontofieldnotebooks,anumberofdocumentsareavailablefor trackingsamplecustody. Documentsincludingsamplecustodysealsandchainofcustodyrecordswillbeobtainedfromthe RSCCinEPA sregion10qualityassuranceoffice.chainofcustodyprocedureswillbeusedto maintainanddocumentsamplecollectionandpossession.aftersamplepackaging,theappropriate chainofcustodyformwillbecompleted.scribesoftwarewillbeusedforprojectdatamanagement andcompletingchainofcustodydocumentation. CopiesoftheTRCOC,ScribeXML(*.xml)andExcel(*.xls)aresubmittedtoCLPandtheRSCCin accordancewiththeinstructionsforsampleshippinganddocumentationperclp/rscc requirements.thelaboratorycopyistobesenttotheclpandsubcontractedlabs,whilethe regionalcopyistobesenttomel.allscribeprojectinformation,sampleinformation,and documentation(labels/trcocs)mustbecompletedaccordingtotheregion10rsccsampling guidelines.aseparateuniquetrafficreport(tr)/chainofcustodywillbecreatedforeachcooler shipped,documentingthespecificcontentsandlocationoftheassociatedcooler. SEA /ES SEA 3-1

26 SECTION 3 DATA GENERATION AND ACQUISITION (EPA GROUP B) Thefollowingsubsectionssummarizeeachelementofsamplehandlingandcustody,asapplicableto splitsgeneratedbytai slaboratoryfollowinghomogenizationandprocessingofsamples Chain-of-Custody Becausesamplescollectedduringanyinvestigationcouldbeusedasevidence,theirpossession mustbetraceablefromthetimethesamplesarecollecteduntiltheyareintroducedasevidencein legalproceedings.chainofcustodyproceduresarefollowedtodocumentsamplepossession Definition of Custody Asampleisundercustodyifoneormoreofthefollowingcriteriaaremet: Thesampleisinaperson sphysicalpossession. Thesampleisinaperson sviewafterbeinginhisorherphysicalpossession. Thesamplewasinaperson sphysicalpossessionandwasthenlockeduporsealedtoprevent tampering. Thesampleiskeptinadesignatedsecuredarea Field Custody Doesnotapply TAIwillretaincustodyofthesamplesinthefield Transfer of Custody and Shipment Samplesareaccompaniedbyachainofcustodyrecord.Whentransferringsamples,theindividuals relinquishingandreceivingthesamplessign,date,andnotethetimeontherecord.thisrecord documentscustodytransferfromthesampler,oftenthroughanotherperson,totheanalystatthe laboratory. Samplesarepackagedproperlyforshipmentanddispatchedtotheappropriatelaboratoryfor analysis,withaseparatechainofcustodyrecordaccompanyingeachshippingcontainer(onefor eachfieldlaboratoryifbeingusedandoneforsamplesdriventothelaboratory).shipping containerswillbesealedwithcustodysealsforshipmenttothelaboratory.couriernamesandother pertinentinformationareenteredinthe Receivedby sectionofthechainofcustodyrecord.the RSCCwillbenotifiedofshipmentandtheScribe.xmlfilewillbeuploadedtotheCLPSample ManagementOffice(SMO)PortalWebsiteonthedayofshipment. Allshipmentsareaccompaniedbythechainofcustodyrecordidentifyingitscontents.Theoriginal recordandonecopyaccompanytheshipmenttothelaboratory,andasecondcopyisretainedby thepm.thescribe.xmlfileisalso edtothersccalongwithther10templatecustomview.xls fileexport. AseparateuniqueTR/COCandAirbillwillbecreatedforeachcoolershipped,documentingthe specificcontentsandlocationoftheassociatedcooler.freightbills,postalservicereceipts,andbills ofladingareretainedaspartofthepermanentdocumentation Laboratory Custody Procedures Adesignatedsamplecustodianacceptscustodyoftheshippedsamplesandverifiesthatthesample numbersmatchthoseonthechainofcustodyrecords.pertinentinformationaboutshipment, pickup,andcourierisenteredinthe Remarks section.thecustodianthenentersthesample numbersintoaboundnotebook.thelaboratorycustodianusesthesampleidentificationnumberor assignsauniquelaboratorynumbertoeachsample,andisresponsibleforensuringthatallsamples aretransferredtotheproperanalystorstoredintheappropriatesecurearea. 3-2 SEA /ES SEA

27 SECTION 3 DATA GENERATION AND ACQUISITION (EPA GROUP B) Thecustodiandistributessamplestotheappropriateanalysts.Laboratorypersonnelareresponsible forthecareandcustodyofsamplesfromthetimetheyarereceiveduntilthesampleisexhaustedor returnedtothecustodian.thedatafromsampleanalysesarerecordedonthelaboratoryreport form. WhensampleanalysesandnecessaryQCcheckshavebeencompletedinthelaboratory,theunused portionofthesamplewillberetaineduntilspecificwrittenpermissionfordisposalisreceivedfrom EPA.Theunusedportionofthesamplewillthenbedisposedofproperly.Allidentifyingsampletie tags,datasheets,andlaboratoryrecordsareretainedaspartofthedocumentation.sample containersandremainingsamplesaredisposedofbythelaboratoryincompliancewithallfederal, state,andlocalregulatoryrequirements Custody Seals Custodysealswillbeplacedoncoolersduringtransportofsamplestothelaboratory.Thesealswill beplacedontwosidesofthelid(oneinfront,andoneontheside)andcoveredwithtapeto preventinadvertentbreakingoftheseals.topreventtheopeningofcoolersduringshipmentandto ensurethatthesamplesremainsealedundercustodyuntilarrivalatthelabadditionallargeliner bag(drumlinertype)insidearoundentirecontentsofcooler(iceandsamples),tiedtightlyclosed andsecuredwithadditionalcustodysealwillalsobeused Field Notebooks Aspartoffieldoversight(seeAppendixB),fieldnotebooksandformswillbeusedtorecord observationsmadeduringphase2sedimentsamplingactivities.fieldnotebookswillalsobeused bypersonnelpickingupandshippingthesplitsamplesfromtai slaboratory.thenotebookwillbe retainedbyeachagencyasapermanentrecord,andcopiesoffieldnotesfromeachsamplingevent willbesubmittedtoepa. Thesenotebooksareintendedtoprovidesufficientdataandobservationstoenableparticipantsto reconstructeventsthatoccurredduringtheproject,andtorefreshthememoryofthepersonnel,if required Corrections to Documentation Alloriginaldatarecordedinfieldnotebooksandfielddataformswillbewritteninwaterproofink, unlessprohibitedbyweatherconditions.noneoftheseaccountableserializeddocumentsistobe destroyedorthrownaway,eveniftheyareillegibleorcontaininaccuraciesthatrequirea replacementdocument. Ifanerrorismadeonanaccountabledocument,personnelmaymakecorrectionssimplybydrawing asinglelinethroughtheerrorandenteringthecorrectinformation.theerroneousinformation shouldnotbeobliterated.anysubsequenterrordiscoveredonanaccountabledocumentshouldbe correctedbythepersonwhomadetheentry.allsubsequentcorrectionsmustbeinitialedand dated. 3.4 Analytical Methods (B4) Projectanalytes,methodsandtargetlaboratorydetectionlimitsarelistedinTable22and23. Whereapplicable,sampleswillbeanalyzedthroughEPAContractLaboratoryProgram(CLP)andthe associatedstatementsofworkalongwithclpqa/qcrequirements.dependingonavailability, theseanalysesmayalsobecarriedoutthroughtheeparegionallaboratory,mel,permelsopsand SEA /ES SEA 3-3

28 SECTION 3 DATA GENERATION AND ACQUISITION (EPA GROUP B) QA/QCprocedures.WhereCLPisnotapplicablesampleswillbeanalyzedbytheEPAregional laboratory(mel)permelsopsandqa/qcprocedures. 3.5 Quality Control (B5) Field Quality Control Procedures Sincethesampleswillbecollectedatthelaboratoryfollowinghomogenizationandprocessing,no fieldqcsampleswillbecollected Laboratory Procedures LaboratoryQCprocedureswillincludethefollowing: AnalyticalmethodologyandQCaccordingtomethodslistedinTable22 InstrumentcalibrationandstandardsasdefinedinthemethodslistedinTable22and laboratory(mel)sops Laboratoryblankmeasurementsataminimum5percentor1perbatchfrequency Accuracyandprecisionmeasurementsataminimumof1in20,1perset DatareductionandreportingaccordingtothemethodslistedinTable22 LaboratorydocumentationperMELstandardoperatingprocedureequivalenttoEPAContract Laboratory(CLP)documentation. 3.6 Instrument/Equipment Testing, Inspection, and Maintenance (B6) NotapplicableTAIwillbecollectingthesamplesthatwillbesplitfollowingprocessingatthe laboratory. 3.7 Instrument/Equipment Calibration and Frequency (B7) Field Calibration Procedures Notapplicable Laboratory Calibration Procedures LaboratorycalibrationproceduresarespecifiedinthemethodsreferencedinTable22andinthe laboratory ssop. 3.8 Inspection/Acceptance of Supplies and Consumables (B8) Suppliesandconsumableswillbeacquiredandinspectedinaccordancewithacquisition specificationsuponreceipt. 3.9 Non-direct Measurements (B9) Notapplicable;analyticalresultsforthesplitsampleswillbecompareddirectlytoanalyticalresults forparentsamplescollectedundertheepaapprovedphase2sedimentqapp. 3-4 SEA /ES SEA

29 SECTION 3 DATA GENERATION AND ACQUISITION (EPA GROUP B) 3.10 Data Management (B10) Splitsampleanalyticaldatawillundergolaboratorydatareviewandverification.Splitsampledata managementisdiscussedfurtherinsection5(epagroupd)ofthisqapp.followingreceiptof reviewedandvalidateddata,thedatawillbeuploadedtoepa selectronicdatawarehouse(wqx) tofacilitatedataaccess,queries,andreportpreparation.datamanagementpracticesaredetailedin theprojectdatamanagementplan(ch2mhill,2011d).scribesoftwarewillbeusedtodocument andmanagesplitsamplecustody,locationinformation,andotherdataassociatedwithanysamples submittedforchemicalanalysis. SEA /ES SEA 3-5

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31 SECTION 4 4 Assessment and Oversight (EPA Group C) Thissectiondescribesassessment,oversight,andreportstomanagement. 4.1 Assessments and Response Actions (C1) TheQAO,RTL,andPMwillmonitortheperformanceoftheQAprocedures.Ifproblemsariseorthe EPATOPOdirectsthePMaccordingly,theQAOwillconductauditsofsplitsamplecollectionand documentationprocedures.fieldauditsmaybescheduledtoevaluatethefollowing: Executionoffieldmeasurements Whetherfieldinformationgatheringprocedureswereproperlyimplemented Executionofsampleidentification,chainofcustodyprocedures,fieldnotebooks,sampling procedures,andfieldmeasurements Whethertrainedpersonnelstaffedthesampleevent Whetherequipmentwasinproperworkingorder Availabilityofpropersamplingequipment Whetherappropriatesamplecontainers,samplepreservatives,andtechniqueswereused Whethersamplepackagingandshipmentwereappropriate WhetherQCsampleswereproperlycollected ChemicalanalyseswillbecarriedoutatEPAMELoranEPACLPlaboratory.Analyses,ifneeded,may alsobecarriedoutatsubcontractlabsasdirectedbyrscc.thedistributionofanalysestothe laboratorieswillbedeterminedaccordingtolaboratorycapabilityandcapacityandthesampling schedule.thedistributionofanalysesmaychangeatthetimeofanalysisdependingoncapacityand implementationofspecificproceduresattheregionallaboratory.therscc,residingatepa s EnvironmentalServicesUnit(ESU),willberesponsibleforcoordinatingandschedulinganalytical servicesfromtheclpsandmel.thedataqualityandlaboratoryperformanceofclplaboratories aremonitoredbytheanalyticalservicesbranchinepaheadquartersandtheregion squalitystaff, includingtheclppoandrscc.formel,qaoversightisprovidedbythelaboratory sqa Coordinator.Inaddition,onsiteauditsorperformanceevaluationsampleswillbeadministeredby thech2mhillqaoandeparegionalqao,asnecessary.auditswillbefollowedupwithanaudit reportpreparedbythereviewer.theauditorwillalsodebriefthelaboratoryorthefieldteamatthe endoftheauditandrequestthatthelaboratoryorfieldteamcomplywiththecorrectiveaction request. IfQCauditsresultindetectionofunacceptableconditionsordata,thePMwillberesponsiblefor developingandinitiatingcorrectiveaction.thetopowillbenotifiedifnonconformanceisof programsignificanceorrequiresspecialexpertisenotnormallyavailabletotheprojectteam.insuch cases,thepmwilldecidewhetheranycorrectiveactionshouldbepursued.correctiveactioncould includethefollowing: SEA /ES SEA 4-1

32 SECTION 4 ASSESSMENT AND OVERSIGHT (EPA GROUP C) Recollectingfielddataifpracticable Evaluatingandamendingfielddatacollectionprocedures Reanalyzingsamplesifholdingtimecriteriapermit Resamplingandanalyzing Evaluatingandamendingsamplingandanalyticalprocedures Acceptingdataacknowledgingalevelofuncertainty Allcorrectiveactionswillbedocumentedinafieldlogbook. 4.2 Reports to Management (C2) ThePMorTOPOmayrequestthataQAreportbemadetotheTOPOontheperformanceofsample collectionanddataquality.thereportwillincludethefollowing: Assessmentofmeasurementdataaccuracy,precision,andcompleteness Resultsofperformanceaudits Resultsofsystemsaudits SignificantQAproblemsandrecommendedsolutions 4-2 SEA /ES SEA

33 SECTION 5 5 Data Validation and Usability (EPA Group D) Thissectionintroducestheconceptsofdatareview,verification,andvalidation;describes verificationandvalidationmethods;andexplainsreconciliationwithuserrequirements. 5.1 Data Review, Verification, and Validation (D1) Dataforallparameters(exceptMELdata)willundergotwolevelsofreviewandvalidation:(1)atthe laboratorydatareviewandverification,and(2)outsidethelaboratorybythirdpartyindependent dataverificationandvalidation.clpgenerateddatawillbeverifiedandvalidatedbythequality StaffinEPA sesupriortoauthorizationofpaymenttothelaboratory.thedatageneratedbythe regionalepalaboratory(mel)isreviewedandverifiedinternallyatmelandisnotconsidered validation althoughvalidationqualifiersareappliedasneeded.ifneeded,theepar10qaunitmay validatemeldataforuniquecircumstanceswhereitisrequested.allvalidatedclplaboratorydata aredownloadeddirectlybych2mhillinthesmoportalandasneeded edbyepaqato CH2MHILL.ThestageofvalidationassignedtoeachSampleDeliveryGroup(SDG)willdetermine whenthedataarefinalandappropriatefordownloadandprojectuse(seesection5.2).thedata generatedbythesubcontractedcommerciallaboratorieswillbevalidatedbych2mhilloran independentthirdpartydatareviewer.stageofdatavalidationasexplainedbelowwillbeincluded inthedatavalidationreport. 5.2 Verification and Validation Methods (D2) Initiallaboratoryanalyticaldatareduction,validation,andreportingatthelaboratorywillbe performedasdescribedinthelaboratoryspecificsops.independentdatavalidationbyepaortheir designeeandsubcontractedlaboratorydatavalidationbych2mhillwillfollowepacontract LaboratoryProgramNationalFunctionalGuidelinesforInorganic/OrganicDataReview(EPA,2002b and2010),asdescribedabove.ch2mhillvalidationofsubcontracteddataformethodsotherthan CLPorCLPequivalent(e.g.,Method6010)willfollowEPAguidanceasapplicabletomethodQC parameters(e.g.,astmmethods).anequivalentlevelofeffortasprescribedintheguidancewillbe implemented.theminimumlevelofeffortforsubcontracteddatavalidationwillbeat10%s2bve and90%s4vem. EPAvalidationofCLPdataislabeledwithalevelofeffort Stage identificationinaccordancewith GuidanceforLabelingExternallyValidatedLaboratoryAnalyticalDataforSuperfundUse(EPA, 2009b).Standardizedterminologyforidentificationofdatavalidationisdesignedtohelpincrease nationalconsistencyandimprovecommunicationandunderstandingaboutthenatureof verificationandvalidationconductedonlaboratoryanalyticaldataforsuperfunduse.anindepth definitionofeachdatavalidationstagelabelcanbefoundinappendixaofthecitedepaguidance document. InorganicdataiselectronicallyvalidatedatS2BVE;howeverorganicdataisautomaticallyvalidated ats3vethroughvalidationsoftwarepriortodeliveryatthesmoportal.forthisproject,afullstage 4electronicandmanualdatavalidation(100percentS4VEM)willbeperformedonallsamplesifQA resourcesandtimeareavailable(epa,2009b). SEA /ES SEA 5-1

34 SECTION 5 DATA VALIDATION AND USABILITY (EPA GROUP D) AllEDDswillbedownloadedbytheprojectstaff/designatedcontractorsfromtheCLPSMOPortal. EPAQAchemistswillnotifytheprojectdatamanagerswithSMOPortalaccesswhenSDGsare designatedforvalidation(30percent).thosedesignatedsdgsarenotfinaluntiltheepaqadata ValidationReporthasbeensentoutandthedatareflectthe S4VEM DVlabel.AllotherS2BVE(70 percent)oftheprojectdatamaybedownloadedaftersiteuploadhasoccurred.validationreport memorandumsandqualifiedresultswillbepreparedbythevalidator(epas4vemorclpsmo S2BVE)andsubmittedtotheEPAPMandthecontractor spms. 5.3 Reconciliation with User Requirements (D3) LaboratoryanalyticaldataobtainedwillbereconciledwiththerequirementsspecifiedinTable22. Assessmentofdataforprecision,accuracyandcompletenesswillbeperformedinaccordancewith thequantitativedefinitionsinthefollowingsections. Splitsampledatawillbeevaluatedasdescribedinstep5oftheDQO,AppendixA Precision Ifcalculatedfromduplicatemeasurements,usethefollowingequation: Where: (C1 C2 ) x 100% RPD = (1) (C + C ) / RPD = relativepercentdifference C 1 = largerofthetwoobservedvalues C 2 = smallerofthetwoobservedvalues Ifcalculatedfromthreeormorereplicates,userelativestandarddeviation(RSD)ratherthanthe RPD,asfollows: Where: RSD = ( s / y ) x 100 % (2) RSD = relativestandarddeviation s = standarddeviation y = meanofreplicateanalyses Standarddeviation,s,isdefinedasfollows: Where: n 2 ( yi / y ) S = _ (3) n 1 i = 1 s = standarddeviation y i = measuredvalueofthei th replicate y = meanofreplicateanalyses n = numberofreplicates 5-2 SEA /ES SEA

35 SECTION 5 DATA VALIDATION AND USABILITY (EPA GROUP D) Accuracy Formeasurementswherematrixspikesareused,usethefollowing: Where: S U % R 100 % x (4) C sa %R = percentrecovery S = measuredconcentrationinspikedaliquot U = measuredconcentrationinunspikedaliquot C sa = actualconcentrationofspikeadded Forsituationswhereastandardreferencematerial(SRM)isusedinsteadoforinadditiontomatrix spikes,usethefollowing: Where: C m % R 100% x (5) C sm %R = percentrecovery C m = measuredconcentrationofsrm C sm = actualconcentrationofsrm Completeness (Statistical) Definedasfollowsforallmeasurements: Where: V % C 100% x T (6) %C = percentcompleteness V = numberofmeasurementsjudgedvalid T = totalnumberofmeasurements SEA /ES SEA 5-3

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37 SECTION 6 6 References MacDonald,D.D.,C.G.Ingersoll,andT.A.Berger.2000.Developmentandevaluationofconsensus basedsedimentqualityguidelinesforfreshwaterecosystems.arch.environ.contam.toxicol. 39: TeckAmericanInc.(TAI).2013.UpperColumbiaRiverDraftQualityAssuranceProjectPlanforthe Phase2SedimentStudy.PreparedforTeckAmericanIncbyExponent,Hydroqualand Parametrix,Inc.March2013. EPA.2001.EPARequirementsforQAProjectPlans(QA/R5).(EPA/600/R98018).Washington,D.C. U.S.EnvironmentalProtectionAgency.Reissued2006. EPA.2002a.EPAGuidanceforQualityAssuranceProjectPlans.(QA/G5).(EPA/240/R02009). Washington,D.C.U.S.EnvironmentalProtectionAgency. EPA,2002b.EPAContractLaboratoryProgramNationalFunctionalGuidelinesforInorganic/Organic DataReview(EPA,2002band2010), EPA.2003a.EPAProgramPlanforRegion10ArchitectEngineeringServices,ContractSolicitationNo. PRR U.S.EnvironmentalProtectionAgency. EPA.2003b.EPAManagementPlansandStandardOperatingProceduresforRegion10Architect EngineeringServices,ContractSolicitationNo.PRR U.S.EnvironmentalProtection Agency. EPA.2005.UniformFederalPolicyforQualityAssuranceProjectPlans.Part1UFPQAPPManual, Final,Version1.U.S.EnvironmentalProtectionAgency.March2005. EPA.2006.GuidancefortheDataQualityObjectivesProcess(QA/G4).EPA/600/R96/066.U.S. EnvironmentalProtectionAgency.Washington,D.C. EPA.2008c.TestMethods. EnvironmentalProtectionAgency. EPA.2009a.Region10(R10)QualityManagementPlan. EPA.2009b.EPA.2009b.GuidanceforLabelingExternallyValidatedLaboratoryAnalyticalDatafor SuperfundUse.EPA540R08005,OSWERDirectiveNo January13.U.S. EnvironmentalProtectionAgency. EPA.2009cNationalRecommendedWaterQualityCriteria.U.S.EnvironmentalProtectionAgency, OfficeofWater.Availableat EPA,2010.SuperfundAnalyticalServices/ContractLaboratoryProgram. EnvironmentalProtectionAgency. EPA,2011.CLPSamplersGuide SEA /ES SEA 6-1

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39 Appendix A Data Quality Objectives

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41 APPENDIX A Data Quality Objectives for UCR Phase 2 Sediment Study Split Sample Metals Analysis Step 1. Problem Statement DQO Guidance of Purpose and Outputs of Step Purpose: Clearly define the problem that requires new environmental data so that the focus of the study will be clear and unambiguous. Outputs From This Step: A concise description of the problem. A list of the planning team members and identification of the decision-maker. A summary of available resources and relevant deadlines for the study. Sediment Study Split Sample Metals Analysis Program Background Samples collected during the UCR Phase 2 Sediment Study will be analyzed by a laboratory subcontracted to TAI. Under such circumstances, there is a potential for bias in the analyses and reporting of results. The EPA will obtain splits of designated sediment samples and analyze them using the same preparation and analytical methods as the TAI laboratory in order to identify possible bias and, if found, evaluate its significance. Problem Statement Samples collected during the UCR Phase 2 Sediment Study will be analyzed by a laboratory subcontracted to TAI. As part of the oversight activities there is a need for the EPA to obtain split samples towards assessing (1) comparability of results - precision (2) sample homogeneity and splitting efficiency and (3) laboratory performance. Planning Team Members Laura Buelow and Matt Wilkening - EPA TOPOs Gina Greppo-Grove/EPA Quality Assurance Manager Marilyn Gauthier - CH2M HILL Project Manager Artemis Antipas - CH2M HILL Quality Assurance Officer Limitations on Available Resources Collection of split samples will follow field sampling activities and laboratory processing (sieving and splitting) conducted by others. Split samples must be obtained and analyzed within appropriate holding times for designated analyses. Relevant Deadlines CH2M HILL needs to submit all relevant planning documents for approval prior to the start of the sampling event, currently scheduled for September 3 through October 31, A-1

42 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 2. Decision Statements DQO Guidance of Purpose and Outputs of Step Purpose: Define the decision(s) that will be resolved using data to address the problem. Approach: Identify the key question that the study attempts to address and alternative actions that may be taken, depending on the answer to the key study question. Outputs From This Step: A statement of the decision that must be resolved using data to address or solve the problem. A list of possible actions or outcomes that would result from each resolution of the decision statement. Note from EPA guidance on DQO: If the principal study question is not obvious and specific alternative actions cannot be identified, then the study may fall in the category of exploratory research, in which case, this particular step of the DQO Process may not be needed. Sediment Study Split Sample Metals Analysis Program Key Questions Are the analytical results reported by TAI s laboratory in agreement with those reported by EPA to include: (1) comparability of results precision; (2) sample homogeneity and splitting efficiency; and (3) laboratory performance (both EPA's and TAI's). Possible Outcomes TAI s laboratory results are significantly different than EPA s laboratory TAI s laboratory results are not significantly differently than EPA s laboratory A-2

43 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 3. Inputs to the Decision DQO Guidance of Purpose and Outputs of Step Purpose: The purpose of this step is to identify the data inputs that will be required to resolve the decision, and to determine which inputs require environmental measurements. Activities: Identify the information that will be required to resolve the decision. Determine the sources for each item of information identified. Identify the information that is needed to establish the action level for the study. Confirm that appropriate field sampling techniques and analytical methods exist to provide the necessary data. Outputs From This Step: A list of informational inputs (including sources and potential action levels) needed to resolve the decision. The list of environmental variables or characteristics that will be measured. Sediment Study Split Sample Metals Analysis Program Informational Needs Split sample data analyzed by the EPA and TAI independently for the same analytes and methods as those identified in TAI s QAPP (UCR Phase 2 Sediment Study QAPP, TAI 2013). Sources of Information EPA split sample analyses TAI split sample analyses Action Levels Detection levels need to be the same for TAI and EPA samples; thus the detection limits identified in TAI s QAPP will be targeted. The ecological screening values that define the target analytical detection limits for TAI are listed in Table 2-3 of the Split Sample Metals Analysis QAPP Field and Analytical Methods Sample preparation protocols and analytical methods are listed in Table 2-2 of the QAPP. A-3

44 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 4. Study Boundaries DQO Guidance of Purpose and Outputs of Step Purpose: Specify the spatial and temporal circumstances that are covered by the decision. Activities: Define the domain or geographic area within which all decisions must apply. Specify the characteristics that define the population of interest. When appropriate, divide the population into strata that have relatively homogeneous characteristics. Define the scale of decisionmaking. Determine when to collect data. Determine the timeframe to which the study data apply. Identify any practical constraints on data collection. Outputs From This Step: Characteristics that define the domain of the study. A detailed description of the spatial and temporal boundaries of the decision. A list of any practical constraints that may interfere with the study. Sediment Study Split Sample Metals Analysis Program Spatial The spatial boundary of the study is shown in Figure 2-3. Split samples will be obtained within this boundary and the findings will be applied to the full study within this boundary. i.e. While comparisons of the laboratory data will be on a sample by sample basis, the decisions about the quality of laboratory data may apply to the entire study-area, to certain analytical methods, or to other possible stratifying elements. Temporal Decision will apply until the data are evaluated and the next round of sampling is planned. Practical Constraints on Data Collection Some sediment sample locations may not have sufficient volume to allow for splitting, thus constraining the number of samples that might be randomly selected for duplicate analysis. A-4

45 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 5. Decision Rules DQO Guidance of Purpose and Outputs of Step Purpose: The purpose of this step is to integrate the outputs from previous steps into a single statement that describes the logical basis for choosing among alternative actions. Activities: Specify the parameter that characterizes the population of interest. Specify the action level for the study. Combine the outputs of the previous DQO steps into an if then decision rule that defines the conditions that would cause the decisionmaker to choose among alternative actions. Outputs From This Step: An if then statement that defines the conditions that would cause the decisionmaker to choose among alternative courses of action. Sediment Study Split Sample Metals Analysis Program Specify the parameter that characterizes the population of interest Individual sample/analyte concentrations Specify the action level for the study same as Step 3 Develop a decision rule: Split sample data for EPA and TAI data will be obtained for assessing (1) comparability of results - precision (2) sample homogeneity and splitting efficiency and (3) laboratory performance (both EPA's and TAI's). The following processes will be used, as needed, to compare and evaluate split sample data: 1- Both EPA and TAI data will be validated and flagged per EPA data validation guidelines. 2-Split sample data will be tabulated to include for both TAI and EPA data, sample collection dates and locations, sample IDs, sample results, detection limits, laboratory and validation flags. 3-For initial comparison of split sample data, two factors will be calculated and entered into the split sample data table described above: 3a- Relative percent deviation (RPD), which is calculated by taking the difference of the two split sample results divided by the average and multiplied by one hundred. The RPD results will be screened to an advisory limit of 50% and the data flagged for RPDs exceeding 50%. 3b-Agreement factor, which is obtained by dividing the detected results for each sample by each other. Depending on the situation and agreement factor (see table below), the split sample results will be categorized in the split sample data tables as being in agreement, disagreement; or major disagreement. Situation Agreement Disagreement Major Disagreement Analyte detected above RL in both TAI and EPA samples < 2X difference >2X < 3X difference >3X difference One result is less than RL < 3X difference >3X< 5X difference >5X difference One result is less than MDL < 5X difference >5X < 10X difference >10X difference RL- reporting limit, MDL- method detection limit Derived from Reference: CRREL Special Report No. 96-9, Comparison Criteria for Environmental Chemical Analyses of Split Samples Sent to Different Laboratories - Corps of Engineers Archived Data, Grant, C.G., Jenkins, T.F., and Mudambi, A.R., USACE Cold Regions & Environmental Research Laboratory, Hanover NH, May1996. The number of agreement, disagreement and major disagreement observations can be further evaluated for the following: Numbers of sample pairs compared A-5

46 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step DQO Guidance of Purpose and Outputs of Step Sediment Study Split Sample Metals Analysis Program Number of sample pairs with one detect and one nondetect (then percentages of each) Number of sample pairs with both non-detects (then percentages of each). Number of sample records with two detections (and percentages) Number of pairs and the percentage of those pairs that fall into the following categories: major disagreement disagreement acceptable The findings for the above analyses can be summarized to assess potential biases. 4- Further statistical evaluations as needed following the above evaluations may be carried out to assess potential biases in the data sets.. 5-Comparisons to project action levels (regulatory or risk criteria) for reporting limits and detects both sets of data can also be compared to project action levels for potential indication of bias for either set. The above framework provides for an overall view of the agreement between the EPA and TAI results. A-6

47 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 6. Tolerable Limits on Decision Rules DQO Guidance of Purpose and Outputs of Step Purpose: Specify the decision-maker's acceptable limits on decision errors, which are used to establish appropriate performance goals for limiting uncertainty in the data. Activities: Determine the possible range of the parameter of interest. Define both types of decision errors and identify the potential consequences of each. Specify a range of possible parameter values where the consequences of decision errors are relatively minor (gray region). Assign probability values to points above and below the action level that reflect the acceptable possibility for the occurrence of decision errors. Check the limits on decision errors to ensure that they accurately reflect the decision-maker's concern about the relative consequences for each type of decision error. Outputs From This Step: The decision-maker's acceptable decision error rates based on a consideration of the consequences of making an incorrect decision. Sediment Study Split Sample Metals Analysis Program This step is not applicable as locations of split samples will be per systematic judgment as described in the next step. A-7

48 APPENDIX A DATA QUALITY OBJECTIVES FOR UCR PHASE 2 SEDIMENT STUDY SPLIT SAMPLE METALS ANALYSIS Step 7. Optimization of the Sampling Design DQO Guidance of Purpose and Outputs of Step Purpose: Identify the most resource-effective sampling and analysis design for generating data that are expected to satisfy the DQOs. Activities: Review the DQO outputs and existing environmental data. Translate the information from the DQOs into a statistical hypothesis. Develop general sampling and analysis design alternatives. For each design alternative, formulate the mathematical expressions needed to solve the design problems. For each design alternative, select the optimal sample size that satisfies the DQOs. Select the most resourceeffective design that satisfies all of the DQOs. Document the operational details and theoretical assumptions of the selected design in the Sampling and Analysis Plan. Outputs From This Step: The most resource-effective design for the study that is expected to achieve the DQOs, selected from a group of alternative designs generated during this step. Sediment Study Split Sample Metals Analysis Program The intent of the split sample analysis program is to duplicate the sample preparation and analyses being conducted by TAI s laboratory on a randomly selected group of sediment samples from the study area. Samples will be collected at 15 percent of the Phase 2 sample locations One hundred and forty (140) locations along the UCR will be sampled for chemical analysis during the course of the Phase 2 Sediment Study. The split samples will be obtained from the homogenized sediment at 15 percent of the sediment sample locations (approximately 21 locations). The samples to be split will be following homogenization of samples from the sediment sampling locations listed in Table 2-5 of the QAPP. If a listed primary sample location cannot be sampled, the split will be obtained at the successful alternate for that location. If there are no successful alternates for the location, a replacement split sample location will be randomly selected from remaining pool of primary sampling locations. The selection process will be repeated until splits are collected at 15% of the total number of successful sample locations (21 locations). A-8

49 Appendix B Field Oversight Plan

50

51 APPENDIXB UCR Phase 2 Sediment Sampling (2013) - Field Oversight and Split Sample Collection Plan ThisdocumentprovidestheFieldOversightandSplitSampleCollectionPlanforobservationanddocumentation ofthephase2sedimentfieldsamplingeffortsbeingconductedbyteckamericanincorporated(tai)during2013. TAIisapotentiallyresponsibleparty(PRP)fortheUpperColumbiaRiver(UCR)siteandwillberesponsiblefor collection,processing,andanalysisofsedimentsamplesduringthescheduledsamplingevent.thesampling programbeingconductedbytaiisdescribedinthequalityassuranceprojectplanforthephase2sediment Study(Phase2SedimentQAPP)(TAI,2013)SubjecttoEPAapproval,fieldsamplingisexpectedtobegininearly tomidfall(septembertooctober)2013andtakeapproximately6to8weeks.ch2mhillpersonnelwillprovide workwithu.s.environmentalprotectionagency(epa)stafftoprovidefieldoversightofsedimentandporewater samplingprocedures.detailsconcerningcollectionanddocumentationofthesplitsamplesarepresentedinthe Phase2SedimentStudySplitSampleMetalsAnalysisQualityAssuranceProjectPlan(SplitSampleMetalsQAPP; CH2MHILL,2013).EPAsplitsampleswillnotbecollectedinthefieldandCH2MHILLpersonnelarenotexpected totakepossessionofanysplitsamplesinthefield.theu.s.departmentofinterior(doi)willcollectsplitsamples withintheirjurisdictionasrequiredintaissamplingpermit. Summary of 2013 Sampling Event Thissectionprovidesageneralsummaryoftheplanned2013sedimentsamplingevent.Fieldoversightpersonnel willberesponsibleforreviewingthephase2sedimentqappandthesplitsamplemetalsandbioassayqappsand willbefamiliarwiththedetailsofthesamplecollection,processing,anddocumentationprogramsdetailed thereinbeforegoinginthefield(tai,2013;appendixa). TheprimarygoaloftheUCRPhase2sedimentstudyistoevaluateifthereareunacceptableriskstobenthic invertebratesassociatedwithexposuretometalsandotherchemicals,collectivelycalledchemicalsofpotential concern(copcs)inucrsediments(tai,2013).sedimentsamplingforphase2toxicitytestingaspartoftheucr RemedialInvestigation/FeasibilityStudy(RI/FS)isintendedtotargetsedimentwithatleast25percentofthe samplegrainsizelessthan2millimeters(mm),representingdepositionalareastoassessriskstobenthic organisms. ConsistentwithinputfromtheEPA,TAIwillattempttocollectwholesedimentandfieldporewaterat140target samplingstationsfromthetop6in.(15cm)ofthesedimentfortheanalysisofanalyticalchemistry.these140 targetstationsinclude124sitestations(includes10internalreferencestations),6tributaryreferencestations (previouslysampledin2005),and10upstreamreferencestationsincanada(table1).inadditiontochemical analysis,standardshortertermchronicsedimentbioassays(withsurvivalandgrowthendpoints)usinghyalella aztecaandchironomusdilutuswillbeperformedonwholesedimentsamplesfrom74ofthe140locations. Reproductiveendpointswillbeassessedusinglongtermbioassaysonarchivedsedimentfrom18ofthe74 bioassaylocations. Duringthecourseofsampling,fieldconditionsorcircumstancesmayadverselyaffectsamplingsuccess.Such conditionsorcircumstancesmayinclude,butarenotnecessarilylimitedto,thepresenceofculturalresources (refertoculturalresourcescoordinationplan,appendixdoftheqapp),thepresenceofcoarsesubstrates(e.g., gravels,cobbles,boulders,bedrock),and/oraboveaverageriverflowconditions.toaccommodatesuch circumstances,reservesamplingstationshavebeenidentifiedforthe124primarystations,aslistedintable2.in theeventthatsamplescannotbecollectedfromaprimarysamplingstation,thefieldsamplingcrewwillattempt tocollectasamplefromoneofthereservestationsdesignatedasapplicabletothetargetstation. EPAoversightistoensurethatthesamplingisdoneaccordingtotheQAPP.EPAwillmakekeydecisionsonwhen therehavebeenenoughunsuccessfulattemptsataprimarystationtomovetoareservestation. PAGE 1

52 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table1 NumberofUCRSedimentSampleLocations SamplingLocations SampleType Analyses Primary Reserve Site ChemistryandBioassay 48 ChemistryOnly Reference Internal ChemistryandBioassay Tributary ChemistryandBioassay 6 Upstream(Canada) ChemistryandBioassay 10 Totals KeyPoints: EPAandEPArepresentativeswillbeonthesafetyboat(downstreamofOnionCreek)whileNPSandCCT culturalresourceobserverswillbeonthesamplingboatprovidingculturaloversight.thesafetyboatwill maintainradioandvisualcontactwiththeresearchboatatalltimes. ThereisflexibilityintheQAPPtodeterminehowmanyattemptsshouldbemadeatonelocationbefore movingtoareservestation.generally,threegrabsatthreedifferentanchorpoints(foratotalof9grabs) shouldbeattemptedbeforeconsideringmoving.ifthefirstgrabrefusesorisotherwiseobviouslynot suitable,thentaiisnotrequiredtodo2moregrabs.theycanmoverightaway.ifafterthe9grabsthere isonlyasmallamountofsedimenttogathertogettherequiredvolume,thenepashouldinstructtaito stayonthatlocationandcontinuetotakemoregrabs.useyourbestjudgmentinthefield.communicate withepaintheothersafelyboatasneeded. TheNPSandCCTpermitrequireTAItocollectanadditional35gallonsofsplitsampleforeachbioassay station.epaandnpsneedtoworktogetherinthefieldtodeterminewhentomovetoareservestation. Generally,EPAwilldeterminewhentomovetoanotherstationiftheinitial12gallonscannotbe collected.oncethe12gallonsiscollectedtosatisfytheqapp,npsthendetermineshowmucheffort shouldbemadetogettheadditional35gallonsfornps.inthesituationthattaicannotget15gallons thentheyhavetorequestawaiverfromtheparkservice,whichcanbegivenverballybythenps representativeinthefield. Generally,keepsedimentfromaprimarysiteevenifitisdecidedtomovetoareservesite.TAImayaskif theyhavetodisposeofthesample,andepaisinsupportofkeepingasmuchsampleastheyareableto getatalllocations.oncethesamplingiscomplete,wecandecidewhichsampleswillbeanalyzed.this decisiondoesnotneedtobemadeinthefield. Thesamplescanbetakenanywhereina150 radiusfromthecenterofthesamplingpoint.thefirst sampledoesnothavetobetakeninthecenter.epashouldbeincommunicationwiththeresearchboat andtogetherdeterminewheretoattemptthefirstsample. SupporttheCulturalMonitors.Oneachresearchboat,therewillbeaculturalmonitorfromtheNPS, ColvilleTribeorSpokaneTribe.Theyhaveaprotocoltofollowifanyartifactorsuspectartifactisfound. Followtheirleadastowhetherthesamplelocationismovedtowithintheallowed150 radiusor completelyabandonedandareservestationisselected.also,npsparticularlyhasspecifichoursthatthey canwork(notmorethan12hperday,includingtravel).iftheirhoursareup,weneedtofollowtheirlead andnotputpressureonanyonetocontinuetowork. PAGE 2

53 Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Primary Bioassay 1 1B1 1R1,1R5,1R2,1R10,1C1 1R9,2R9,2R10 Primary Bioassay 1 1B2 1R9,1C2,1BR1,1BR2,1BR3, 1BR4 2R1,2BR2,1R1,1R2,1R5,1R7,1R8,1R10 Primary Bioassay 1 1B3 3R10,1R3,1R6,2R2,2R4,2R7 Primary Bioassay 1 1B4 1R9,1C2,1BR1,1BR2,1BR3, 1BR4 2R1,2BR2,1R1,1R2,1R5,1R7,1R8,1R10 Primary Bioassay 1 1B5 1R7,1R8,1BC1,1C3 3R4,1R9 Primary Bioassay 1 1B6 1R10,1R2,1R1,1R5,1C1 1R9,2R9,2R10 Primary ChemistryOnly 1 1C1 1R1,1R5,1R2,1R10 2R9,2R10,1R9,1BR1,1BR2,1BR3,1BR4 Primary ChemistryOnly 1 1C2 1R9,1BC2,1BC3,1BC4 2R1,2BR2,1BR1,1BR2,1BR3,1BR4,3R10,1 R1,1R2,1R5,1R7,1R8,1R10 Primary ChemistryOnly 1 1C3 1R7,1R8,1BC1 3R4,1R9 Primary ChemistryOnly 1 1C4 1R3,1R4 3R3,1R6,2R2,2R3,2R4,2R5,2R6,2R7 Reserve bioassayorchem 1 1R1 Reserve bioassayorchem 1 1R2 Reserve bioassayorchem 1 1R3 Reserve bioassayorchem 1 1R4 Reserve bioassayorchem 1 1R5 Reserve bioassayorchem 1 1R6 Reserve bioassayorchem 1 1R7 Reserve bioassayorchem 1 1R8 Reserve bioassayorchem 1 1R9 Reserve bioassayorchem 1 1R10 Primary ChemistryOnly 1B 1BC1 1R7,1R8,3R4,1BR1,1BR2,1BR3,1BR4 Primary ChemistryOnly 1B 1BC2 1BR1,1BR2,1BR3,1BR4 Primary ChemistryOnly 1B 1BC3 1BR1,1BR2,1BR3,1BR4 Primary ChemistryOnly 1B 1BC4 1BR1,1BR2,1BR3,1BR4 1R9,2R1,2BR2,3R1,3R6,1R1,1R2,1R5,1 R10,1R7,1R8 1R9,2R1,2BR2,3R1,3R6,1R1,1R2,1R5,1 R10,1R7,1R8 1R9,2R1,2BR2,3R1,3R6,1R1,1R2,1R5,1 R10,1R7,1R8 Reserve bioassayorchem 1B 1BR1 Reserve bioassayorchem 1B 1BR2 PAGE 3

54 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 1B 1BR3 Reserve bioassayorchem 1B 1BR4 Primary Bioassay 2 2B1 2R1,2BR2 Primary Bioassay 2 2B2 Primary Bioassay 2 2B3 Primary Bioassay 2 2B4 2R2,2R4,2R5,2R6,2R7,2 R32C1,2C3,2C2 2R2,2R4,2R5,2R6,2R7,2 R32C1,2C3,2C2 2R2,2R4,2R5,2R6,2R7,2 R32C1,2C3,2C2 1BR1,1BR2,1BR3,1BR4,3R2,3R6,4R7,2R9, 2R10 1R6,3R1,3R7,3R8,3R9 1R6,3R1,3R7,3R8,3R9 1R6,3R1,3R7,3R8,3R9 Primary Bioassay 2 2B5 2R10,2R9 1R10,1R5,1R1,1R2,3R5,2R1,2BR2 Primary Bioassay 2 2B6 2R10,2R9 1R10,1R5,1R1,1R2,3R5,2R1,2BR2 Primary ChemistryOnly 2 2C1 Primary ChemistryOnly 2 2C2 Primary ChemistryOnly 2 2C3 2R2,2R4,2R5,2R6,2R7,2 R3 2R3,2R2,2R4,2R5,2R6,2 R7 2R2,2R4,2R5,2R6,2R7,2 R3 2BR1,2BR3,2BR4,1R6 2BR1,2BR3,2BR4,1R6 2BR1,2BR3,2BR4,1R6 Primary ChemistryOnly 2 2C4 2BR3,2BR4,1R3,1R4,3R3 Reserve bioassayorchem 2 2R1 Reserve bioassayorchem 2 2R2 Reserve bioassayorchem 2 2R3 Reserve bioassayorchem 2 2R4 Reserve bioassayorchem 2 2R5 Reserve bioassayorchem 2 2R6 Reserve bioassayorchem 2 2R7 Reserve bioassayorchem 2 2R8 Reserve bioassayorchem 2 2R9 Reserve bioassayorchem 2 2R10 Primary ChemistryOnly 2B 2BC1 2BR1 2R2,2R4,2R5,2R6,2R7 Primary ChemistryOnly 2B 2BC2 2BR1 2R2,2R4,2R5,2R6,2R7 Primary ChemistryOnly 2B 2BC3 2BR3,2BR4 1R3,1R4,3R3 Primary ChemistryOnly 2B 2BC4 2BR1 2R2,2R4,2R5,2R6,2R7 PAGE 4

55 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 2B 2BR1 Reserve bioassayorchem 2B 2BR2 Reserve bioassayorchem 2B 2BR3 Reserve bioassayorchem 2B 2BR4 Primary Bioassay 3 3B1 3R1,3R7,3R8,3R9,3C1 2R3,2BR1,4R2,4R6,2R2,2R4,2R5,2R6,2 R7,2R8 Primary Bioassay 3 3B2 3R5 2R9,2R10,3R6,3R2,3R10 Primary Bioassay 3 3B3 3R4 3R2,3R6,2R1,2BR2,1BR1,1BR2,1BR3,1B R4,4R7 Primary Bioassay 3 3B4 3R5 2R9,2R10,3R6,3R2,3R10 Primary Bioassay 3 3B5 3R2,3R6 2R1,2BR2,4R7,3R4,3R5 Primary Bioassay 3 3B6 3R7,3R8,3R9,3R1,3C1 Primary ChemistryOnly 3 3C1 3R7,3R8,3R9,3R1 2BR1,4R2,4R6,2R2,2R4,2R5,2R6,2R7,2 R3 2BR1,4R2,4R6,2R2,2R4,2R5,2R6,2R7,2 R3 Primary ChemistryOnly 3 3C2 3R3 2BR3,2BR4,4R1 Primary ChemistryOnly 3 3C3 3R3 2BR3,2BR4,4R1 Primary ChemistryOnly 3 3C4 3R3 2BR3,2BR4,4R1 Reserve bioassayorchem 3 3R1 Reserve bioassayorchem 3 3R2 Reserve bioassayorchem 3 3R3 Reserve bioassayorchem 3 3R4 Reserve bioassayorchem 3 3R5 Reserve bioassayorchem 3 3R6 Reserve bioassayorchem 3 3R7 Reserve bioassayorchem 3 3R8 Reserve bioassayorchem 3 3R9 Reserve bioassayorchem 3 3R10 Primary ChemistryOnly 3B 3BC1 3BR1,3BR2,3BR3,3BR4 3R1 Primary ChemistryOnly 3B 3BC2 3BR1,3BR2,3BR3,3BR4 3R1 Primary ChemistryOnly 3B 3BC3 3BR1,3BR2,3BR3,3BR4 3R1 PAGE 5

56 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Primary ChemistryOnly 3B 3BC4 3BR1,3BR2,3BR3,3BR4 3R1 Reserve bioassayorchem 3B 3BR1 Reserve bioassayorchem 3B 3BR2 Reserve bioassayorchem 3B 3BR3 Reserve bioassayorchem 3B 3BR4 Primary Bioassay 4 4B1 3R4,4R7,4C4,2R1,2BR2,3R2,3R6 Primary Bioassay 4 4B2 4R7,4C4 Primary Bioassay 4 4B3 4R7,4C4 Primary Bioassay 4 4B4 4R1,4R3,4R4,4R5,4R9,4 R11,4R10,4R12 Primary Bioassay 4 4B5 3R2,3R6,2R1,2BR2,1BR1,1BR2,1BR3,1B R4 3R2,3R6,2R1,2BR2,1BR1,1BR2,1BR3,1B R4 5R5,5R6 3R5,2R9,2R10,4R7,4C4,4R1,4R11,4R3,4 R4,4R5,4R9,4R10,4R12 Primary Bioassay 4 4B6 4R2,4R6 3R7,3R8,3R9 Primary ChemistryOnly 4 4C1 Primary ChemistryOnly 4 4C2 Primary ChemistryOnly 4 4C3 4R5,4R9,4R11,4R3,4R4,4 R10,4R12,4R1 4R12,4R10,4R5,4R9,4R11, 4R4,4R3,4R1, 4R4,4R3,4R1,4R9,4R5,4 R10,4R12,4R11 Primary ChemistryOnly 4 4C4 4R7 Primary ChemistryOnly 4 4C5 4R5,4R9,4R11,4R3,4R4,4 R10,4R12,4R1 5R5,5R6 5R1,5R7,3R10 5R5,5R6 3R2,3R6,3R4,3R5,3R10,4R1,4R3,4R4,4R 5 3R10,5R9,5R5 Primary ChemistryOnly 4 4C6 4R2,4R6 3R1,3R7,3R8,3R9,4R1,4R3,4R4,4R5 Reserve bioassayorchem 4 4R1 Reserve bioassayorchem 4 4R2 Reserve bioassayorchem 4 4R3 Reserve bioassayorchem 4 4R4 Reserve bioassayorchem 4 4R5 Reserve bioassayorchem 4 4R6 Reserve bioassayorchem 4 4R7 Reserve bioassayorchem 4 4R9 PAGE 6

57 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 4 4R10 Reserve bioassayorchem 4 4R11 Reserve bioassayorchem 4 4R12 Primary ChemistryOnly 4B 4BC1 4BR1 5R8,3R3 Primary ChemistryOnly 4B 4BC2 4BR2,4BR3,4BR4 4R11,4R5,4R9 Primary ChemistryOnly 4B 4BC3 4BR2,4BR3,4BR4 4R11,4R5,4R9 Primary ChemistryOnly 4B 4BC4 4BR4,4BR2,4BR3 4R5,4R9,4R11 Reserve bioassayorchem 4B 4BR1 Reserve bioassayorchem 4B 4BR2 Reserve bioassayorchem 4B 4BR3 Reserve bioassayorchem 4B 4BR4 Primary Bioassay 5 5B1 5R8 4BR1,6R4,6R5,6R9 Primary Bioassay 5 5B2 5R5,5R6,5R7,5R9,5R1 4R1,4R9 Primary Bioassay 5 5B3 5R5,5R6,5R7,5R9,5R1 4R1,4R9 Primary Bioassay 5 5B4 5R5,5R6,5R7,5R9,5R1 4R1,4R9 Primary Bioassay 5 5B5 5R3,5R10,5C2 5BR1,5R1,5R5,5R6,5R7,5R8,5R9 Primary Bioassay 5 5B6 5R3,5R10,5C2 5BR2,5BR3,5BR4,4R6,5R1,5R5,5R6,5R7, 5R9,5R8 Primary ChemistryOnly 5 5C1 5R5,5R6,5R7,5R9,5R1 4R9,4R11 Primary ChemistryOnly 5 5C2 5R3,5R10 5BR2,5BR3,5BR4,4R6,5R1,5R5,5R6,5R7, 5R9,5R8 Primary ChemistryOnly 5 5C3 5R5,5R6,5R7,5R9,5R1 4R9,4R11 Primary ChemistryOnly 5 5C4 5R5,5R6,5R7,5R9,5R1 4R9,4R11 Reserve bioassayorchem 5 5R1 Reserve bioassayorchem 5 5R2 Reserve bioassayorchem 5 5R3 Reserve bioassayorchem 5 5R4 Reserve bioassayorchem 5 5R5 Reserve bioassayorchem 5 5R6 Reserve bioassayorchem 5 5R7 PAGE 7

58 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 5 5R8 Reserve bioassayorchem 5 5R9 Reserve bioassayorchem 5 5R10 Primary ChemistryOnly 5B 5BC1 5BR2,5BR3,5BR4,5BR1 6R1,6R3,6R6,6R7,6R10,5R3,5R10 Primary ChemistryOnly 5B 5BC2 5BR2,5BR3,5BR4,5BR1 6R1,6R3,6R6,6R7,6R10,5R3,5R10 Primary ChemistryOnly 5B 5BC3 5R8,6R5,6R9,6C1,6C3,3R3 Primary ChemistryOnly 5B 5BC4 5BR2,5BR3,5BR4,5BR1 6R1,6R3,6R6,6R7,6R10,5R3,5R10 Reserve bioassayorchem 5B 5BR1 Reserve bioassayorchem 5B 5BR2 Reserve bioassayorchem 5B 5BR3 Reserve bioassayorchem 5B 5BR4 Primary Bioassay 6 6B1 6R3,6R6,6R7,6R10,6R1 5BR1,7R4,7R5,7R10,5BR2,5BR3,5BR4 Primary Bioassay 6 6B2 6R8,6R2 5R7,7R8 Primary Bioassay 6 6B3 6R3,6R6,6R7,6R10,6R1 5BR1,7R4,7R5,7R10,5BR2,5BR3,5BR4 Primary Bioassay 6 6B4 6R4,6R5,6R9,6C1,6C3 6BR1,6BR2,6BR3,6BR4 Primary Bioassay 6 6B5 6R3,6R6,6R7,6R10,6R1 5BR1,7R4,7R5,7R10,5BR2,5BR3,5BR4 Primary Bioassay 6 6B6 6R3,6R6,6R7,6R10,6R1 5BR1,7R4,7R5,7R10,5BR2,5BR3,5BR4 Primary ChemistryOnly 6 6C1 6R4,6R5,6R9 6BR1,6BR2,6BR3,6BR4 Primary ChemistryOnly 6 6C2 6R8,6R2 5R7,7R8 Primary ChemistryOnly 6 6C3 6R4,6R5,6R9 6BR1,6BR2,6BR3,6BR4 Primary ChemistryOnly 6 6C4 6R8,6R2 5R7,7R8 Reserve bioassayorchem 6 6R1 Reserve bioassayorchem 6 6R2 Reserve bioassayorchem 6 6R3 Reserve bioassayorchem 6 6R4 Reserve bioassayorchem 6 6R5 Reserve bioassayorchem 6 6R6 Reserve bioassayorchem 6 6R7 Reserve bioassayorchem 6 6R8 PAGE 8

59 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 6 6R9 Reserve bioassayorchem 6 6R10 Primary ChemistryOnly 6B 6BC1 6R3,6R6,6R7,6R10,6R1 Primary ChemistryOnly 6B 6BC2 6BR1,6BR3,6BR4,6BR2 7R1,7R2,6R4,6R5,6R9 Primary ChemistryOnly 6B 6BC3 6BR1,6BR3,6BR4,6BR2 7R1,7R2,6R4,6R5,6R9 Primary ChemistryOnly 6B 6BC4 6R1,6R3,6R6,6R7,6R10 Reserve bioassayorchem 6B 6BR1 Reserve bioassayorchem 6B 6BR2 Reserve bioassayorchem 6B 6BR3 Reserve bioassayorchem 6B 6BR4 Primary Bioassay 7 7B1 7R1,7R2,7R3,7R9,7C1 7BR1,7BR2,6BR1,6BR3,6BR4 Primary Bioassay 7 7B2 7R6,7R7,7R4,7R5,7R10 6R1,6R3,6R6,6R7,6R10,7BR4 Primary Bioassay 7 7B3 7R4,7R5,7R10,7R6,7R7 7BR4,6R3,6R6,6R7,6R10 Primary Bioassay 7 7B4 7R1,7R2,7R3,7R9,7C2 6BR2,7BR1,7BR2 Primary Bioassay 7 7B5 7R8 6R8,8R1,8R3 Primary Bioassay 7 7B6 7R4,7R5,7R10,7R6,7R7 7BR4,6R3,6R6,6R7,6R10 Primary ChemistryOnly 7 7C1 7R1,7R2,7R3,7R9 7BR1,7BR2,6R4,6R5 Primary ChemistryOnly 7 7C2 7R1,7R2,7R3,7R9 7BR1,7BR2,6R4,6R5 Primary ChemistryOnly 7 7C3 7R8 6R2,6;R8,8R3 Primary ChemistryOnly 7 7C4 7R8 6R2,6;R8,8R3 Reserve bioassayorchem 7 7R1 Reserve bioassayorchem 7 7R2 Reserve bioassayorchem 7 7R3 Reserve bioassayorchem 7 7R4 Reserve bioassayorchem 7 7R5 Reserve bioassayorchem 7 7R6 Reserve bioassayorchem 7 7R7 Reserve bioassayorchem 7 7R8 Reserve bioassayorchem 7 7R9 PAGE 9

60 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 7 7R10 Primary ChemistryOnly 7B 7BC1 7BR4 7R4,7R5,7R6,7R7,7R10 Primary ChemistryOnly 7B 7BC2 7BR4 7R4,7R5,7R6,7R7,7R10 Primary ChemistryOnly 7B 7BC3 7BR3 8BR1,8R2,8R3,8R4 Primary ChemistryOnly 7B 7BC4 7BR3 8BR1,8R2,8R3,8R4 Reserve bioassayorchem 7B 7BR1 Reserve bioassayorchem 7B 7BR2 Reserve bioassayorchem 7B 7BR3 Reserve bioassayorchem 7B 7BR4 Primary Bioassay 8 8B1 8R2,8R10,8R6,8R9 Primary Bioassay 8 8B2 8R6,8R9,8R10,8R2 8BR1,7R4,7R5,7R6,7R7,7R10,8R1,8R3,8 R4,8R5,8R7 8BR1,7R4,7R5,7R6,7R7,7R10,8R1,8R3,8 R4,8R5,8R7 Primary Bioassay 8 8B3 8R8,8R1,8R5,8R3,8R4 8BR3,8BR4,7R8,8R2,8R6,8R9,8R10 Primary Bioassay 8 8B4 8R6,8R9,8R10,8R2 Primary Bioassay 8 8B5 8R6,8R9,8R10,8R2 8BR1,7R4,7R5,7R6,7R7,7R10,8R1,8R3,8 R4,8R5,8R7 8BR1,7R4,7R5,7R6,7R7,7R10,8R1,8R3,8 R4,8R5,8R7 Primary Bioassay 8 8B6 8R8,8R1,8R5,8R3,8R4 8BR3,8BR4,7R8,8R2,8R6,8R9,8R10 Primary ChemistryOnly 8 8C1 8R7 7BR2,7BR1,7R1,7R2 Primary ChemistryOnly 8 8C2 8R7 7BR2,7BR1,7R1,7R2 Primary ChemistryOnly 8 8C3 5R2,5R4,8R3 Primary ChemistryOnly 8 8C4 8R8,8R1,8R5,8R3,8R4 7R8,7BR3 Reserve bioassayorchem 8 8R1 Reserve bioassayorchem 8 8R2 Reserve bioassayorchem 8 8R3 Reserve bioassayorchem 8 8R4 Reserve bioassayorchem 8 8R5 Reserve bioassayorchem 8 8R6 Reserve bioassayorchem 8 8R7 Reserve bioassayorchem 8 8R8 PAGE 10

61 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve bioassayorchem 8 8R9 Reserve bioassayorchem 8 8R10 Primary ChemistryOnly 8B 8BC1 8BR1 8R6,8R9,8R10 Primary ChemistryOnly 8B 8BC2 8BR2 8R7,7BR1,7BR2,8R2,8R6,8R9,8R10 Primary ChemistryOnly 8B 8BC3 8BR3,8BR4 8R1,8R3,8R4,8R5,8R8 Primary ChemistryOnly 8B 8BC4 8BR2 8R7,7BR1,7BR2,8R2,8R6,8R9,8R10 Reserve bioassayorchem 8B 8BR1 Reserve bioassayorchem 8B 8BR2 Reserve bioassayorchem 8B 8BR3 Reserve bioassayorchem 8B 8BR4 Primary InternalReference Ref1 Ref1b Primary InternalReference Ref2 Ref2b Primary InternalReference Ref3 Ref3b Primary InternalReference Ref4 Ref4b Primary InternalReference Ref5 Ref5b Primary InternalReference Ref6 Ref6b Primary InternalReference Ref7 Ref7b Primary InternalReference Ref8 Ref8b Primary InternalReference Ref9 Ref9b Primary InternalReference Ref10 Ref10b Reserve InternalReference Ref1b Reserve InternalReference Ref2b Reserve InternalReference Ref3b Reserve InternalReference Ref4b Reserve InternalReference Ref5b Reserve InternalReference Ref6b Reserve InternalReference Ref7b Reserve InternalReference Ref8b Reserve InternalReference Ref9b PAGE 11

62 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Table2 SampleLocationInformationByFocusAreaandLocationPriority LocationPriority ProposedAnalysis FocusArea PrimarySample ID Reserve1 (samegroupandfocusareaas primarysample) Reserve2 (potentiallydifferentgroupordifferentfocus Area) Reserve InternalReference Ref10b Primary TributaryReference Trib1 Primary TributaryReference Trib2 Primary TributaryReference Trib3 Primary TributaryReference Trib4 Primary TributaryReference Trib5 Primary TributaryReference Trib6 PAGE 12

63 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Summary of Sample Collection Procedures FieldsamplingmethodsaredetailedintheFieldSamplingPlan(AppendixAofthePhase2SedimentStudyQAPP) andsummarizedbelow: OnboatActivities: 1. Deploydecontaminatedgrabsampleratsamplingstation.RecordGPSlocation. 2. Retrievegrabsamplerandcheckgrabsamplerforsampleacceptability(e.g.,closedsampler,not overfilled,minimalwinnowing,photographsedimentinthesampler. 3. Ifacceptancecriteriaarenotmet,classifythissedimentasrejected.Rejectedsedimentshouldbe processedasdescribedbelowandtemporarilystoredduringattemptstocollectacceptedsediment. 4. Siphonwaterfromsampler. 5. Extractporewaterfromthegrabsamplerintoasyringeusinganairstone(labelsyringeascontaining porewaterfromrejectedoracceptedsediments). 6. DepositsedimentintoLexantub. 7. Examinesedimentforthepresenceofculturalresources(performedbytribalorNationalParkService culturalresourcemonitors).iftherecoveredsedimentcontainsculturalresources,followinstructions fromtheculturalresourcemonitorregardingwhattodowiththerecoveredsedimentandcultural artifacts,aswellaswhethertoabandonthesamplingstation. 8. SamplesrejectedduetoincorrectgrabsasdefinedinStep2willnotbeprocessedforchemicalanalysisor toxicitytesting. 9. Evaluateanddocumentsedimentparticlesize(AsdetailedinSOP3,ifthereissufficientvolumeto performanalysespertablea2,sedimentsamplesshouldbeevaluatedandhomogenizedaslaidoutinthe stepsbelow,andretainedforfutureanalyses.thecollectionoftheserejectedsedimentswillallowsome evaluationofthearea,intheeventthatsimilarsamplingdifficultiesareencounteredatreserve locations.) a. Removelargerocksanddebrisfromsedimentsbyhandcontainingmostlyfineparticles. b. Presssedimentthrougha5mmsieveifsedimentscontainlargefractionsofparticles>2mm.Do notuseriverwatertowashsedimentsthroughsieve. c. Assessthesedimentgrainsize(atleast25percentmustbe2mm). 10. Evaluateanddocumentthepresenceofblacksilicaparticlesinsediment. 11. Homogenizesedimentsampleusingdecontaminatedequipment(e.g.,handheldpowerauger). a. NotethatEPAhasdiscussedhowhomogenizationonthesamplingboatmaybechallengingand theadequacyofthisprocedurewillbeaddressedthroughoversightwheretaimustproducea samplethatishomogenizedtotheepaobserver ssatisfaction.iftheyareunabletosatisfythe observerthentherewillbemorediscussionaboutalternatives.beingonaseparateboatwillpose challenges,ofcourse,butepa/ch2mhillwilldothebestwecanandwewillbeontheboat duringinitialsamplingwheretheprocedureisdemonstratedandupstreamofonioncreekwhere thesampleswithhighestmetalconcentrationsarelikelytobecollected.thechemistrydatamay alsoinformourdeterminationoftheadequacyofhomogenization butthisisafterthefact. 12. Photographhomogenizedsediments 13. Fillallsedimentsamplecontainersindesignatedorder.Addriverwatertobioassaysedimentsamplesto createathinwaterlayer.thislayerwillminimizeoxygenationduringtransit. PAGE 13

64 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING a. FilltheAVS/SEMcontainercompletely,leavingnoheadspace.DistributetheAVS/SEM preservativebystoringthecontainerinvertedorbymixing. b. Fillallremainingsedimentcontainersforanalyticalchemistry,minimizingheadspace c. Fillappropriatedecontaminatedbioassaycontainers(e.g.,5gallonbucketswithlids)with sediment. d. Addriverwatertobioassaysedimentsamplestocreateathinwaterlayer.Thislayerwillminimize oxygenationduringtransit. e. Fillporewatersamplecontainerswithfilteredorunfilteredwaterasappropriatefortheanalytical method. 14. Storeallanalyticalchemistrysamplesinacoolerwithice.Bioassaysamplesmaybestoredonthe samplingvesselatambienttemperature[notethatthesewillnotbeseparatedfromtheparentsampleon theboatandwillneedtobemaintainedonice]. 15. Returnanyexcesssedimentand/orporewatertotheriver,decontaminateequipment(e.g.,grabsampler, Lexantub,mechanicalstainlesspaddlewheelmixer),andmovetonextsamplestation. 16. Uponarrivingatthedock,transferallsamplecontainersintoarefrigeratedareawheretheycanbestored untilshipped. 17. Decontaminatesamplecollection,homogenization,andtransferequipmentbeforemovingtothenext samplestation. OnshoreActivities(TAI) 1. Receiveandlogporewaterandsedimentsamples. 2. Storeallsamplecontainersintoarefrigeratedarea(beforeandafterprocessing). 3. Preparesamplelabels,chainofcustodyandshippingdocuments. 4. Packsamplesincoolersandshiptolaboratories. NotethatEPAssplitchemistrysampleandbioassaysampleswillbepreparedbyTAIslab(AustralianLaboratory Service[ALS] formerlycolumbiaanalyticalservices[cas])afterreceivingsamplescollectedinthefield.thelife cycleofasedimentsampleiscurrentlyplannedtobeasdescribedinfigure1. Sample Analysis Theporewaterandsedimentsampleswillbeanalyzedasfollows: PorewaterIfsufficientvolumeisavailable,fieldporewatersampleswillbeanalyzedforTALmetals(the dissolvedfraction)andotherwaterqualityparametersneededtoassessmetalbioavailabilityusingthe BLM.Therefore,thevolumedependentpriorityorderofporewateranalytesaredescribedintheQAPP as:1)aluminum,cadmium,calcium,copper,iron,lead,magnesium,manganese,nickel,potassium, sodium,andzinc;2)ph,dissolvedorganiccarbon[doc],hardness(tobecalculated),andalkalinity;and3) chlorideandsulfate.inadditiontotheaforementionedbioassays,nofewerthan35sedimentsampleswill beselectedforbackscatterelectronmicroscopy.preliminaryresults(e.g.,chemistrydata,field observationsetc.)willbeusedtorefineandidentifywhichsampleswillundergothisevaluation. SedimentSedimentsamplesfromalllocationswillbeanalyzedforgrainsize,pH,acidvolatilesulfide (AVS),simultaneouslyextractedmetals(SEM),totalorganiccarbon(TOC),andtargetanalytelist(TAL) metals.bioassaystobeperformedon74samplesinclude: 28daywholesedimenttoxicitytestswiththeamphipod,H.azteca(endpointsofsurvival,weight,and biomass[usepa2000;astm2011]) PAGE 14

65 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING 10daywholesedimenttoxicitytestswiththemidge,C.dilutus(endpointofsurvival,weight,and biomass[usepa2000;astm2011]) Inadditiontotheabovelistedstandardbioassays,reproductiveendpointswillbealsoassessedon18 splitsamples.consistentwithepa sdirection,preferenceforthese18splitsampleswillbegiventothose stationslocatedwithinhighandmediumexposuregradientbins,butwillbefinalizedfollowinground1 activities.resultsoftheabovelisted10and28daysurvivalandgrowthtests,inconjunctionwith preliminarychemistrydatawillbeusedtorefineandidentifywhichsampleswillundergofurther evaluation.specificbioassaystobeperformedonthese18samplesincludethefollowing: 42daywholesedimenttoxicitytestswiththeamphipod,H.azteca(endpointsofsurvival,weight, biomass,andneonates/survivingfemale[usepa2000;astm2011]) 50to65daywholesedimenttoxicitytestswiththemidge,C.dilutus(endpointsofsurvival,weight, biomass,emergence,eggs/survivingfemale,egghatching,andviabilityofyoungusingtheadopted methodstartingwith7dayoldlarvae[usepa2000;astm2011]) Collection of Split Samples TwoseparatesplitsampleanalysisprogramswillbeconductedaspartofthePhase2sedimentsamplingevent; splitsfortalmetalsanalysisbytheepa smanchesterenvironmentallaboratory(mel)inportorchard, Washington,andsplitsforbioassaystobeconductedbytheUSArmyEngineerResearchandDevelopmentCenter (ERDC)inVickburg,Mississippi. Thesplitsformetalsanalysiswillbeobtainedfromnolessthan15percentofthesedimentsamplelocations(that is,atotalof21locationsifsedimentissuccessfullyobtainedfromall140primary[orreserve]locations).the sampleswillbeanalyzedfortalmetals.eachsplitsampleformetalsanalysiswillcontainnotlessthan200grams andwillbecollectedassplitsofthehomogenizedsediments.sampleswillbesentbytaifromthefieldtoals, homogenized,andthensplitsampleswillbecollectedandsenttomelforepassplitsampleanalysis.ch2mhill oversightpersonnelmaybeneededtoprovideoversightforthecorrecthomogenization,sampling,labeling,and shippingusingtheepa sscribeapplication.splitsamplelocationsforepachemicalanalysesarelistedintable3. SplitsamplesforEPAsbioassayQA/QCanalyseswillbedeterminedbasedonsamplevolumeandinitialsample chemistryafterthesamplingprogramiscomplete.samplesstoredbytaiatalswillberehomogenizedand shippedtoerdconcethesesampleshavebeenidentified.atotalof10splitsedimentsamplescollectedfor ERDCsbioassayQA/QCtesting.Upto7ofthesewillbesitesamplescollectedbetweenOnionCreekandthe CanadaUSborderandupto3splitsampleswillbecollectedfromreferencelocationsinCanada.AswithEPAs splitchemistrysamples,bioassaysplitsampleswillbesentbytaifromthefieldtoals,homogenized,andthen splitsampleswillbecollectedandsenttoerdcforepassplitsampleanalysis.ch2mhilloversightpersonnel maybeneededtoprovideoversightforthecorrecthomogenization,sampling,labeling,andshippingusingthe EPA sscribeapplication. PAGE 15

66 OVERSIGHT AND SPLIT SAMPLE COLLECTION PLAN PHASE 2 SEDIMENT SAMPLING Sieve<5mm/ PAGE 16 Figure1.LifeofaUCRSedimentSample