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1 POLAR COVALENT BOND 1 P HP OXYGEN HIGHER ELECTRO- O WATER MOLECULE HYDROGEN LOWER ELECTRO- H BOND BOND H ELECTRO- = 3.5 ELECTRO- = 2.1 ELECTRO- = 2.1 = E-

2 POLAR COVALENT BOND +/- - 1 P? CHARGE OXYGEN HIGHER ELECTRO- O WATER MOLECULE HYDROGEN LOWER ELECTRO- H BOND BOND H ELECTRO- = 3.5 = E- +? CHARGE ELECTRO- = 2.1 ELECTRO- = 2.1 HIGHLY POLAR

3 I PHOSPHOLIPID BILAYER POLAR SOLUTE = POLAR SOLUTE +/-

4 +/- PHOSPHOLIPID BILAYER BILAYER INHIBITS PASSAGE POLAR SOLUTES = POLAR SOLUTE +/-

5 PHOSPHOLIPID BILAYER WATER POLAR = WATER +/-

6 = WATER +/-

7 = WATER +/- >

8 w WATER MOVES FREELY ACROSS PHOSPHOLIPID BILAYER = WATER +/-

9 IN WATER S SMALL MOLECULE SIZE PERMITS PASSAGE = WATER +/-

10 ^ PHOSPHOLIPID BILAYER INEFFICIENT WATER PASSAGE = WATER +/-

11 AQUAPORIN PROTEINS

12 AP PHOSPHOLIPID BILAYER = WATER +/-

13 = WATER +/- AQUAPORIN PROTEIN

14 = WATER +/- AQUAPORIN PROTEIN

15 > AQUAPORIN PROTEIN = WATER +/-

16 AP WATER MOVES FREELY ACROSS AQUAPORIN PROTEIN = WATER +/-

17 AQUAPORIN PROTEIN

18 AQUAPORIN PROTEIN WATER SPECIFIC CHANNEL PROTEIN AQUAPORIN PROTEIN

19 WC AQUAPORIN PROTEIN = WATER +/-

20 CHANNEL PERMITS WATER PASSAGE WATER SPECIFIC CHANNEL = WATER +/-

21 = WATER +/- CHANNEL PERMITS WATER PASSAGE

22 EF CHANNEL PERMITS WATER PASSAGE = WATER +/-

23 ^ AQUAPORIN PROTEIN EFFICIENT WATER PASSAGE = WATER +/-

24 WATER MEMBRANE TRANSPORT SUMMARY

25 = WATER +/- PHOSPHOLIPID BILAYER

26 = WATER +/-

27 = WATER +/- W

28 IN WATER MOVES FREELY ACROSS PHOSPHOLIPID BILAYER = WATER +/-

29 ^ PHOSPHOLIPID BILAYER INEFFICIENT WATER PASSAGE = WATER +/-

30 = WATER +/- AQUAPORIN PROTEIN

31 = WATER +/- AQUAPORIN PROTEIN

32 W AQUAPORIN PROTEIN = WATER +/-

33 EF WATER MOVES FREELY ACROSS AQUAPORIN PROTEIN = WATER +/-

34 ^ S AQUAPORIN PROTEIN EFFICIENT WATER PASSAGE = WATER +/-

35 = WATER +/- BIOLOGY MEMBRANE

36 ? WATER = WATER MOVES +/- FREELY ACROSS BIOLOGY MEMBRANE

37 QUESTION A WHAT DO BIOLOGISTS CALL WATER PASSAGE ACROSS A MEMBRANE IN RESPONSE TO SOLUTE CONCENTRATION? QUESTION

38 ANSWER ^ OSMOSIS ANSWER

39 OSMOSIS O

40 OSMOSIS

41 OSMOSIS > WATER PASSAGE ACROSS MEMBRANE IN RESPONSE TO SOLUTE CONCENTRATION OSMOSIS

42 OSMOSIS O CELL A CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

43 OSMOSIS W CELL A OSMOSIS CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

44 CELL A OSMOSIS WATER VIA OSMOSIS CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

45 OSMOSIS P CELL A WATER VIA OSMOSIS CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

46 CELL A OSMOSIS PASSIVE CELL B A = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

47 CELL A OSMOSIS ATP EXPENSE: NO CELL B ^ = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

48 TONICITY TERMS T

49 TONICITY

50 TONICITY I SOLUTION SOLUTE CONCENTRATION TONICITY

51 ISOTONIC SOLUTIONS

52 ISOTONIC SOLUTIONS H EQUAL SOLUTE CONCENTRATION ISOTONIC SOLUTIONS

53 HYPOTONIC SOLUTION

54 HYPOTONIC SOLUTION H LOWER SOLUTE CONCENTRATION HYPOTONIC SOLUTION

55 HYPERTONIC SOLUTION

56 HYPERTONIC SOLUTION AP HIGHER SOLUTE CONCENTRATION HYPERTONIC SOLUTION

57 TONICITY TERMS APPLIED

58 CELL A TONICITY CELL B = WATER MOLECULE = MEMBRANE

59 TONICITY E CELL A CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

60 TONICITY I CELL A CELL B EQUAL SOLUTE = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

61 TONICITY ^ + CELL A CELL B ISOTONIC SOLUTIONS = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

62 TONICITY CELL A CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

63 TONICITY G CELL A CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

64 TONICITY ^ + CELL A CELL B GRADIENT = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

65 TONICITY L CELL A CELL B = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

66 TONICITY HY CELL A CELL B LOW SOLUTE = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

67 TONICITY H CELL A CELL B HYPOTONIC SOLUTION = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

68 TONICITY HY CELL A CELL B HYPOTONIC SOLUTION HIGH SOLUTE = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

69 TONICITY > CELL A CELL B HYPOTONIC SOLUTION HYPERTONIC SOLUTION = WATER MOLECULE = POLAR SOLUTE MOLECULE = MEMBRANE

70 HYPOTONIC > % SOLUTIONS & HYPERTONIC SOLUTIONS --- RELATIVE TERMS

71 HYPOTONIC & HYPERTONIC RELATIVE TERMS % B 25% SOLUTE CELL A

72 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY A 25% SOLUTE 50% SOLUTE CELL A CELL B

73 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY B 25% SOLUTE 50% SOLUTE HYPOTONIC CELL A CELL B

74 HYPOTONIC & HYPERTONIC RELATIVE TERMS % C 25% SOLUTE 50% SOLUTE HYPOTONIC HYPERTONIC CELL A CELL B

75 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY B 25% SOLUTE 50% SOLUTE 75% SOLUTE HYPERTONIC CELL A CELL B CELL C

76 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY C 25% SOLUTE 50% SOLUTE 75% SOLUTE HYPOTONIC CELL A CELL B CELL C

77 HYPOTONIC & HYPERTONIC RELATIVE TERMS R 25% SOLUTE 50% SOLUTE 75% SOLUTE HYPOTONIC HYPERTONIC CELL A CELL B CELL C

78 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY B 25% SOLUTE 50% SOLUTE CELL A CELL B

79 HYPOTONIC & HYPERTONIC RELATIVE TERMS R 25% SOLUTE 50% SOLUTE HYPOTONIC HYPERTONIC CELL A CELL B

80 HYPOTONIC & HYPERTONIC RELATIVE TERMS HY B 50% SOLUTE 75% SOLUTE CELL B CELL C

81 HYPOTONIC & HYPERTONIC RELATIVE TERMS > 50% SOLUTE 75% SOLUTE HYPOTONIC HYPERTONIC CELL B CELL C

82 HYPOTONIC ^ SOLUTIONS & HYPERTONIC SOLUTIONS --- RELATIVE TERMS

83 ^ OSMOSIS BONDED WATER VS OSMOSIS FREE WATER

84 OSMOSIS BONDED WATER

85 CELL A OSMOSIS CELL B = WATER MOLECULE POLAR = MEMBRANE

86 CELL A OSMOSIS CELL B W S = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

87 BONDED WATER AT H - O + 8P - 8N 1 P PS + H 1 P SOLUTE POLAR WATER POLAR

88 BONDED WATER H + - 8P O - ATTRACT 1 P PS + 8N H 1 P SOLUTE POLAR WATER POLAR

89 BONDED WATER // H - O + - 8P 8N PS + 1 P H 1 P SOLUTE POLAR WATER POLAR

90 BONDED WATER BW H - O + - 8P PS + 1 P 8N H 1 P SOLUTE POLAR WATER POLAR // = HYDROGEN BOND

91 BONDED WATER H BONDED WATER - O + - 8P PS + 1 P 8N H 1 P SOLUTE POLAR WATER POLAR // = HYDROGEN BOND

92 BW CELL A OSMOSIS CELL B H-BONDS FORM = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

93 CELL A OSMOSIS CELL B BONDED WATER = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

94 CELL A OSMOSIS BONDED WATER CELL B BONDED WATER APPROACHES MEMBRANE BONDED WATER APPROACHES MEMBRANE = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

95 CELL A OSMOSIS BONDED WATER CELL B BONDED WATER APPROACHES MEMBRANE BONDED WATER APPROACHES MEMBRANE = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

96 CELL A OSMOSIS BONDED WATER CELL B BONDED WATER CONTACTS MEMBRANE BONDED WATER CONTACTS MEMBRANE = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

97 OSMOSIS? CELL A BONDED WATER CELL B BONDED WATER DOES NOT CROSS MEMBRANE BONDED WATER DOES NOT CROSS MEMBRANE = WATER MOLECULE POLAR = SOLUTE MOLECULE POLAR = MEMBRANE

98 QUESTION ^ WHY DOES BONDED WATER NOT CROSS THE MEMBRANE? QUESTION

99 OSMOSIS BONDED WATER --- PHOSPHOLIPID BILAYER

100 = WATER +/- PHOSPHOLIPID BILAYER

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