Kirstie Swingle. Project: Lipopolysaccharide induced dynamic lipid organizations: lipid tubules, membrane perforations, and multi-lamellar stacking

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1 Kirstie Swingle Kirstie Swingle Graduate Research Assistant School of Molecular Sciences Arizona State University Tempe, AZ Phone: Education Arizona State University: Expected degree: PhD in Chemistry August 2016-current University of New Mexico: BS in Biology 2014 Major: Biology Minor: Chemistry Gallup High School, New Mexico: 2010 Professional Research and Work Experience Arizona State University: School of Molecular Sciences Biodesign Center for Molecular Design and Biomimetics: Dr. Alexander Green and Dr. Nicholas Stephanopoulos Biodesign Center for Innovations in Medicine: Dr. Neal Woodbury August 2016-current Project: DNA-Peptide conjugates to activate synthetic gene networks Description: This project looks at the design of protein responsive genetic circuits using DNA-peptide conjugates to activate toe-hold switches for diagnostic design. Los Alamos National Laboratory: Department of Energy May 2013-August 2016 Center for Integrated Nanotechnologies: Dr. Gabriel A. Montaño Project: Lipopolysaccharide induced dynamic lipid organizations: lipid tubules, membrane perforations, and multi-lamellar stacking Description: This project analyzed the interactions between lipopolysaccharides (LPS) from gram-negative bacteria and cellular membranes using lipid bilayer assemblies (LBAs). The results indicate lipopolysaccharides are able to induce conformational changes in the lipid bilayer, resulting in the formation of lipid tubules, multi-lamellar sheets, and static holes depending on the environmental ion concentration. This interaction can now be used to pattern lipid bilayer assemblies as well as enhance our understanding of LPS and lipid bilayers in a natural setting. These results led to a peer reviewed publication. Project: Membrane modification of lipid bilayer assemblies using amphiphilic biomolecules (LPS) Description: This project generated chemical patterning methods for biomaterials design. LPS preferentially forms holes in areas of lipid bilayer as opposed to lipid monolayer region. Thus, adding LPS to a patterned array of membrane bilayer/monolayer regions results in holes in only bilayer regions. Further, the pattern of the bilayer/monolayer regions dictates the size and shape of holes resulting in geometric membrane hole formation over the length of the patterns. These holes can then be backfilled with various

2 molecules. This tool can now be used to study biological mechanisms and generate long-range ordered systems in membrane assemblies. These results led to a peer reviewed publication. Project: Effects of curvature on LPS-Membrane interactions Description: This project entails applying the above concepts to 3-D substrates to better understand the biological implications of the LPS/membrane interaction. Current data has shown that the degree of curvature effects LPS-membrane interactions. By changing the degree of curvature, LPS can be stably incorporated into membranes potentially creating bacterial mimics. The same concepts will soon be applied to Giant Unilamellar Vesicles (GUVs) to study the effects of substrates on the system. Project: Polymer Nanocomposites for controlled, dynamic light-harvesting and energy transfer Description: Previous work by our lab demonstrated using short-chain block copolymers as a matrix for controlling the organization of chromophores for light-harvesting and energy transfer in micelles and membrane materials. To extend this model, we are using phresponsive polymers to order and control chromophores and other nanomaterials in non-covalent arrays capable of ph-dependent and potentially ionic strength dependent, energy transfer efficiency as photosynthetic mimics. We are further exploring these polymer nanocomposites in thin film assemblies. IMDEA: Madrid, Spain June - July 2014 Institute of Nanotechnology: Dr. Aitziber Cortajarena Project Title: Formation of multigradient porous surfaces for selective bacterial entrapment Description: This project led to the creation of a multigradient porous surface using the Breath figures approach specifically to immobilize bacteria based on size and charge. Based on the functionalization of the surface, specific types of bacteria were bound and isolated, preventing further growth. This tool ultimately could be used in a clinical setting to sort and classify bacteria in different samples in an easy and inexpensive manner. University of New Mexico August July 2013 Department of Biology: Dr. Richard Cripps Project Title: Tinman and Pannier regulate a Mef2 Shadow Enhancer in the Drosophila heart Description: This project proved that Tinman, an important gene in Drosophila heart formation, is aided by Pannier. Both of these genes were shown to regulate a Mef2 shadow enhancer crucial in proper formation of the heart. These results provide a more detailed mechanism for proper heart formation in Drosophila and can potentially provide insight to heart formation in vertebrates. These results led to a peer reviewed publication. 2

3 Awards/Honors Achievement Rewards for College Scientists (ARCS): Van Denburgh Scholar AAAS/Science Program for Excellence in Science October 2017 University of New Mexico IMSD program May 2013-Dec 2014 Initiative to Maximize Student Diversity in Stem Fields funded by the NIH 2015 I-CAM Biologically Enabled Self-Assembly Travel Award May 2015 SACNAS National Conference Travel Scholarship October 2014 UNM Biology Research Day Honorable Mention for Poster Presentation April 2014 SACNAS National Conference Biology Poster Award Winner October 2013 New Mexico Bridge Scholar August 2010 Submitted Proposals Block copolymer nanocomposites for bioinspired photonic applications : Accepted Oakridge National Laboratory: Small Angle Neutron Scattering Polymer-chlorosome nanocomposites for light-harvesting and energy transfer Accepted Oakridge National Laboratory: Small Angle Neutron Scattering Presentations/Conferences 2015 Rio Grande Symposium on Advanced Materials: Scientific Talk, Albuquerque, NM: Supported Lipid Bilayers to Create Multicomponent Biological Structural Mimics 2015 Biophysical Society Annual Meeting, Baltimore, MD: Scientific Talk: Using Lipopolysaccharides to create 3-D Multicomponent Supported Lipid Bilayers 2015 New Mexico Stem Strong, Santa Fe, NM: Invited Student Panelist: Expectations and Maximizing Benefits of a Scientific Meeting 2014 SACNAS National Conference: Invited Student Panelist, Los Angeles, CA: Negotiating Collaborations - Successful Collaborations Are Made Not Born 2015 SACNAS National Conference: Poster Presentation 2015 New Mexico Stem Strong: Poster Presentation 2015 I-CAM Biologically Enabled Self-Assembly: Poster Presentation 2015 Biophysical Society Annual Meeting: Poster Presentation 3

4 2014 SACNAS National Conference: Poster Presentation 2014 UNM Biology Research Day: Poster Presentation 2013 SACNAS National Conference: Poster Presentation 2013 UNM Biomedical Research Day: Poster Presentation 2013 CINT User Conference: Poster Presentation 2013 DOE Los Alamos National Laboratory Student Symposium: Poster Presentation Memberships and Involvement New Mexico Stem Strong SACNAS Lifetime member Current Student Mentor 2013-Current University of New Mexico IMSD program May 2013-December 2014 University of New Mexico Gateway Mentoring program August 2013-May

5 Publications Mendez, H.M., Stromberg, L.R., Swingle, K.L., Graves, S. W., Montano, G., Mukundan, H., Serogroup-specific interactions of lipopolysaccharides with supported lipid bilayer assemblies SPIE: Biophysic, Biology, and Biophotonics Swingle, K.L., Collins, A.M., Rai, D., Urban, V.S., Montaño, G.A., Dynamic energy transfer in Poly(Butadiene-B-Acrylic Acid) nanocomposites due to ph and ionic strength induced polymer morphology. (In Preparation) Stromberg, L.R., Hengartner, N.W., Swingle, K.L., Moxley, R.A., Graves, S.W., Montaño, G.A., & Mukundan, H. Membrane Insertion for the Detection of Lipopolysaccharides: Exploring the Dynamics of Amphiphile-in-Lipid Assays PLoS One Adams, P.G., Swingle, K.L., Paxton, W.F., Nogan, J.J., Lamoureux, L., Firestone, M., Mukundan, H., & Montaño, G.A. Exploiting lipopolysaccharide-induced deformation of lipid bilayers to modify membrane composition and generate two-dimensional geometric membrane array patterns Scientific Reports Lovato, T.L., Sensibaugh, C.A., Swingle, K.L., Martinez, M.M., & Cripps, R.M. Tinman and Pannier regulate a Mef2 Shadow Enhancer in the Drosophila heart PLoS One Adams, P.G., Lamoureux, L., Swingle, K.L., Mukundan, H., & Montaño, G.A Lipopolysaccharide-induced dynamic lipid membrane reorganization: tubules, perforations, and stacks Biophysical Journal. 106: