Program Analyte Unit Target Limit Absolute SD %

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1 Andrology Sperm Count 1.0E06/mL Peer Group Mean SD 2 Sperm Morphology % normal Peer Group Mean SD 2 Sperm Viability % viable Peer Group Mean SD 2 Endocrinology Cortisol µg/dl Peer Group Mean % of Target 25.0 DHEA Sulphate µg/dl Peer Group Mean SD 3 Estradiol pg/ml Peer Group Mean Combination (max.limit) Estriol - Total ng/ml Peer Group Mean SD 3 Estriol - Unconjugated ng/ml Peer Group Mean SD 3 Follicle Stimulating Hormone mu/ml Peer Group Mean Combination (max.limit) hcg mu/ml Peer Group Mean SD 3 Luteinizing Hormone mu/ml Peer Group Mean Combination (max.limit) Progesterone ng/ml Peer Group Mean Combination (max.limit) Prolactin ng/ml Peer Group Mean Combination (max.limit) T Uptake % Peer Group Mean SD 3 T Uptake - Unit T Uptake Units Peer Group Mean SD 3 Testosterone ng/ml Peer Group Mean Combination (max.limit) Thyroid Stimulating Hormone µu/ml Peer Group Mean SD 3 Thyroxine - Free ng/dl Peer Group Mean SD 3 Thyroxine - Total µg/dl Peer Group Mean Combination (max.limit) Triiodothyronine - Free pg/dl Peer Group Mean Combination (max.limit) Triiodothyronine - Total ng/dl Peer Group Mean SD 3 General Immunology Alpha-1-Antitrypsin mg/dl Peer Group Mean SD 3 Alpha-fetoprotein ng/ml Peer Group Mean SD 3 CMV Viral Load units/ml Peer Group Mean SD 2 CMV Viral Load log10 units/ml Peer Group Mean Fixed Value 0.50 Compliment C3 mg/dl Peer Group Mean SD 3 Compliment C4 mg/dl Peer Group Mean SD 3 C-Reactive Protein mg/dl Peer Group Mean SD 3 HBV Viral Load units/ml or pg/ml Peer Group Mean SD 2 HBV Viral Load log10 units/ml Peer Group Mean Fixed Value 0.50 HCV Viral Load log10 IU/mL Peer Group Mean Fixed Value 0.50 HCV Viral Load IU/mL Peer Group Mean SD 2 High Sensitivity C-Reactive Protein mg/dl Peer Group Mean Combination (max.limit) HIV Viral Load log10 copies/ml Peer Group Mean Fixed Value 0.50 HIV Viral Load copies/ml Peer Group Mean SD 2 Immunoglobulin A mg/dl Peer Group Mean SD 3 AccuTest Evaluation Criteria V1.0 JANUARY 2008

2 Immunoglobulin E U/mL Peer Group Mean SD 3 Immunoglobulin G mg/dl Peer Group Mean % of Target 25.0 Immunoglobulin M mg/dl Peer Group Mean SD 3 Hematology Activated Clotting Time sec Peer Group Mean Combination (max.limit) APTT sec Peer Group Mean % of Target 15.0 Basophils % Peer Group Mean SD 3 Carboxyhemoglobin % Peer Group Mean SD 3 CD14/CD45+ (Leukocyte Common Antigen) % Total Lymphocytes Peer Group Mean SD 3 CD14+ (Monocyte) % Total Lymphocytes Peer Group Mean SD 3 CD19+ (B Lymphocyte Differentiation Antigen) % Total Lymphocytes Peer Group Mean SD 3 CD2+ (Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD20+ (B Lymphocyte Differentiation Antigen) % Total Lymphocytes Peer Group Mean SD 3 CD3/CD16+ (NK Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD3/CD16+/CD56+ (NK Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD3/CD56+ (NK Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD3+ (Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD3+ (Lymphocytes) - Counts /µl Peer Group Mean SD 3 CD3+/CD4+ (Helper Inducer T Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD3+/CD8+ (Cytotoxic Suppressor T Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD4+ (Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD4+ (Lymphocytes) - Counts /µl Peer Group Mean SD 3 CD5+ T Lymphocytes (Non-B-Cells) % Total Lymphocytes Peer Group Mean SD 3 CD8+ (Lymphocytes) % Total Lymphocytes Peer Group Mean SD 3 CD8+ (Lymphocytes) - Counts /µl Peer Group Mean SD 3 D-Dimer µg/ml Peer Group Mean SD 3 Eosinophils % Peer Group Mean SD 3 ESR mm/hr Peer Group Mean SD 3 F Cell Value % Peer Group Mean SD 2 Fibrinogen mg/dl Peer Group Mean % of Target 20.0 Granulocytes % Peer Group Mean SD 3 Hematocrit % Peer Group Mean % of Target 6.0 Hematocrit (Spun & Conductivity) % Peer Group Mean Combination (max.limit) Hemoglobin g/dl Peer Group Mean % of Target 7.0 Hemoglobin (Waived) g/dl Peer Group Mean Combination (max.limit) Hemoglobin F - Quantitative % Peer Group Mean SD 2 INR Peer Group Mean SD 3 Lymphocytes % Peer Group Mean SD 3 Lymphocytes/Monocytes (Centrifugal) 1.0E09/L Peer Group Mean SD 3 Mean Corpuscular Volume fl Peer Group Mean Combination (max.limit) Methemoglobin % Peer Group Mean SD 3 AccuTest Evaluation Criteria V1.0 JANUARY 2008

3 Monocytes % Peer Group Mean SD 3 Neutrophils % Peer Group Mean SD 3 Oxyhemoglobin % Peer Group Mean SD 3 Platelets 1.0E03/uL Peer Group Mean % of Target 25.0 Prothrombin Time sec Peer Group Mean % of Target 15.0 Red Blood Cell Count 1.0E06/uL Peer Group Mean % of Target 6.0 Red Blood Cell Count (Body Fluid) /µl Peer Group Mean SD 3 Red Cell Distribution Width % Peer Group Mean Combination (max.limit) Reduced Hemoglobin % Peer Group Mean SD 3 Reticulocyte Count % Peer Group Mean SD 2 White Blood Cell Count 1.0E03/uL Peer Group Mean % of Target 15.0 White Blood Cell Count (Body Fluid) /µl Peer Group Mean SD 3 Routine Chemistry 11-Deoxycortisol ng/dl Peer Group Mean SD 2 17-Hydroxycorticosteroids mg/l Peer Group Mean SD 2 17-Hydroxyprogeserone ng/dl Peer Group Mean SD 2 17-Ketogenic Steroids mg/l Peer Group Mean SD 2 3-Methoxytyramines µg/l Peer Group Mean SD 2 5-Hydroxindoleacetic acid mg/l Peer Group Mean SD 2 Acetone mg/dl Peer Group Mean SD 2 ACTH pg/ml Peer Group Mean SD 2 Alanine Aminotransferase U/L Peer Group Mean % of Target 20.0 Albumin g/dl Peer Group Mean % of Target 10.0 Albumin (CSF, Urine & Body fluid) mg/dl or g/dl or mg/l Peer Group Mean SD 3 Albumin (Electrophoresis) % Peer Group Mean SD 3 Albumin (Electrophoresis) g/dl Peer Group Mean SD 3 Albumin/Creatinine Ratio mg/g Peer Group Mean SD 3 Aldosterone µg/l Peer Group Mean SD 2 Alkaline Phosphatase U/L Peer Group Mean % of Target 30.0 Alpha 1 Globulin (Electrophoresis) % Peer Group Mean SD 3 Alpha 1 Globulin (Eletrophoresis) g/dl Peer Group Mean SD 3 Alpha 2 Globulin (Electrophoresis) % Peer Group Mean SD 3 Alpha 2 Globulin (Electrophoresis) g/dl Peer Group Mean SD 3 Ammonia µmol/l Peer Group Mean SD 3 Amylase U/L Peer Group Mean % of Target 30.0 Amylase (Urine & Body Fluid) U/L Peer Group Mean SD 3 Amylase - Pancreatic U/L Peer Group Mean % of Target 30.0 Androstenedione ng/dl Peer Group Mean SD 2 Apolipoprotein AI mg/dl Peer Group Mean Combination (max.limit) Apolipoprotein B mg/dl Peer Group Mean Combination (max.limit) Aspartate Aminotransferase U/L Peer Group Mean % of Target 20.0 AccuTest Evaluation Criteria V1.0 JANUARY 2008

4 Beta Globulin (Electrophoresis) % Peer Group Mean SD 3 Beta Globulin (Electrophoresis) g/dl Peer Group Mean SD 3 Beta-2-Microglobulin mg/dl Peer Group Mean SD 2 Beta-Hydroxybutyric Acid mg/dl Peer Group Mean SD 3 Bilirubin - Direct mg/dl Peer Group Mean Combination (max.limit) Bilirubin - Direct (Neonatal) mg/dl Peer Group Mean SD 2 Bilirubin - Neonatal mg/dl Peer Group Mean Combination (max.limit) Bilirubin - Total mg/dl Peer Group Mean Combination (max.limit) BNP pg/ml Peer Group Mean SD 3 Calcitonin pg/ml Peer Group Mean SD 2 Calcium mg/dl Peer Group Mean Fixed Value 1.00 Calcium (Urine) mg/dl Peer Group Mean SD 3 Calcium - Ionized mg/dl Peer Group Mean SD 3 Cancer Antigen 125 U/mL Peer Group Mean Combination (max.limit) Cancer Antigen 15-3 U/mL Peer Group Mean % of Target 30.0 Cancer Antigen 19-9 U/mL Peer Group Mean % of Target 30.0 Cancer Antigen U/mL Peer Group Mean SD 2 Carcinoembryonic Antigen ng/ml Peer Group Mean Combination (max.limit) Catecholamines - Free µg/l Peer Group Mean SD 2 Chloride mmol/l Peer Group Mean % of Target 5.0 Chloride (CSF, Sweat & Urine) mmol/l Peer Group Mean SD 3 Cholesterol - HDL mg/dl Peer Group Mean % of Target 30.0 Cholesterol - LDL mg/dl Peer Group Mean Combination (max.limit) Cholesterol - Total mg/dl Peer Group Mean % of Target 10.0 Cholesterol - Total (Body Fluid) mg/dl Peer Group Mean SD 3 CK-MB Activity U/L Peer Group Mean SD 3 CK-MB Mass ng/ml Peer Group Mean SD 3 CO2 - Total mmol/l Peer Group Mean Combination (max.limit) Conductivity mmol/l Peer Group Mean SD 3 Coproporphyrins µg/l Peer Group Mean SD 2 Cortisol - Free µg/l Peer Group Mean SD 2 C-Peptide ng/ml Peer Group Mean SD 2 Creatine Kinase U/L Peer Group Mean % of Target 30.0 Creatinine mg/dl Peer Group Mean Combination (max.limit) Creatinine (Urine) mg/dl Peer Group Mean SD 3 Dopamine µg/l Peer Group Mean SD 2 Epinephrine µg/l Peer Group Mean SD 2 Ferritin ng/ml Peer Group Mean Combination (max.limit) Folate ng/ml Peer Group Mean Combination (max.limit) Fructosamine - mmol/l mmol/l Peer Group Mean SD 2 Fructosamine - umol/l µmol/l Peer Group Mean SD 2 Gamma Globulin (Electrophoresis) % Peer Group Mean SD 3 AccuTest Evaluation Criteria V1.0 JANUARY 2008

5 Gamma Globulin (Electrophoresis) g/dl Peer Group Mean SD 3 Gamma-Glutamyltransferase U/L Peer Group Mean Combination (max.limit) Gastric ph Peer Group Mean Combination (max.limit) 1 2 Gastrin pg/ml Peer Group Mean SD 2 Glucose mg/dl Peer Group Mean Combination (max.limit) Glucose (Urine, CSF & Body Fluid) mg/dl Peer Group Mean SD 3 Glycated Hemoglobin - Total % Peer Group Mean Combination (max.limit) Glycated Hemoglobin A1 % Peer Group Mean Combination (max.limit) HbA1c (A1c) % Peer Group Mean Combination (max.limit) Homocysteine µmol/l Peer Group Mean SD 3 Homovanillic Acid mg/l Peer Group Mean SD 2 IBC - Total µg/dl Peer Group Mean Combination (max.limit) IBC - Unsaturated µg/dl Peer Group Mean % of Target 20.0 Immunoglobulin A (Electrophoresis) mg/dl Peer Group Mean SD 3 Immunoglobulin G (Electrophoresis & CSF) mg/dl Peer Group Mean SD 3 Immunoglobulin M (Electrophoresis) mg/dl Peer Group Mean SD 3 Insulin µu/ml Peer Group Mean SD 2 Insulin-like Growth Factor I ng/ml Peer Group Mean SD 2 Iron µg/dl Peer Group Mean % of Target 20.0 Lactate mmol/l Peer Group Mean Combination (max.limit) Lactate Dehydrogenase U/L Peer Group Mean % of Target 20.0 Lactate Dehydrogenase (Body Fluid) U/L Peer Group Mean SD 3 Lactate Dehydrogenase (CSF) U/L Peer Group Mean SD 2 LD1/LD2 Peer Group Mean % of Target 30.0 Lipase U/L Peer Group Mean % of Target 30.0 Lipoprotein (a) mg/dl Peer Group Mean % of Target 40.0 Magnesium mg/dl Peer Group Mean % of Target 25.0 Magnesium (Urine) mg/dl Peer Group Mean SD 3 Magnesium - Ionized mmol/l Peer Group Mean % of Target 25.0 Metanephrine µg/l Peer Group Mean SD 2 Microalbumin mg/l Peer Group Mean Combination (max.limit) M-Protein (Paraprotein) g/dl Peer Group Mean SD 3 M-Protein (Paraprotein) - % % Peer Group Mean SD 3 Myoglobin ng/ml Peer Group Mean Combination (max.limit) Norepinephrine µg/l Peer Group Mean SD 2 Normetanephrine µg/l Peer Group Mean SD 2 NT-Pro BNP pg/ml Peer Group Mean SD 3 Osmolality mmol/kg Peer Group Mean Combination (max.limit) Parathyroid Hormone pg/ml Peer Group Mean SD 2 pco2 mm Hg Peer Group Mean Combination (max.limit) ph Peer Group Mean Fixed Value 0.04 ph (Body Fluid) Peer Group Mean Fixed Value 1 AccuTest Evaluation Criteria V1.0 JANUARY 2008

6 Phosphorus mg/dl Peer Group Mean Combination (max.limit) Phosphorus (Urine) mg/dl Peer Group Mean SD 3 po2 mm Hg Peer Group Mean SD 3 Potassium mmol/l Peer Group Mean Fixed Value 0.50 Potassium (Urine & CSF) mmol/l Peer Group Mean SD 3 Prealbumin % Peer Group Mean SD 3 Prostate-specific Antigen ng/ml Peer Group Mean Combination (max.limit) Prostate-specific Antigen - Free ng/ml Peer Group Mean SD 3 Prostate-specific Antigen - Total ng/ml Peer Group Mean Combination (max.limit) Prostatic Acid Phosphatase ng/ml Peer Group Mean % of Target 30.0 Protein - Total g/dl Peer Group Mean % of Target 10.0 Protein - Total (CSF, Body Fluid, Urine & Electrophoresis) g/dl Peer Group Mean SD 3 PSA Free/Total Ratio Peer Group Mean SD 3 RBC Folate ng/ml Peer Group Mean SD 2 Sodium mmol/l Peer Group Mean Fixed Value 4.00 Sodium (CSF, Sweat & Urine) mmol/l Peer Group Mean SD 3 Thyroglobulin ng/ml Peer Group Mean SD 2 Transferrin mg/dl Peer Group Mean % of Target 30.0 Triglycerides mg/dl Peer Group Mean % of Target 25.0 Troponin I ng/ml Peer Group Mean Combination (max.limit) Troponin T ng/ml Peer Group Mean Combination (max.limit) Urea/Urea Nitrogen mg/dl Peer Group Mean Combination (max.limit) Urea/Urea Nitrogen (Urine) mg/dl Peer Group Mean SD 3 Uric Acid mg/dl Peer Group Mean % of Target 17.0 Uric Acid (Urine) mg/dl Peer Group Mean SD 3 Uroporphyrin µg/l Peer Group Mean SD 2 Vanillylmandelic Acid mg/l Peer Group Mean SD 2 Vitamin B12 pg/ml Peer Group Mean Combination (max.limit) Toxicology Acetaminophen µg/ml Peer Group Mean Combination (max.limit) Amikacin µg/ml Peer Group Mean Combination (max.limit) Amitriptyline ng/ml Peer Group Mean % of Target 25.0 Caffeine µg/ml Peer Group Mean SD 3 Carbamazepine µg/ml Peer Group Mean % of Target 25.0 Desipramine ng/ml Peer Group Mean % of Target 25.0 Digoxin ng/ml Peer Group Mean Combination (max.limit) Disopyramide µg/ml Peer Group Mean Combination (max.limit) Ethanol mg/dl Peer Group Mean % of Target 25.0 Ethosuximide µg/ml Peer Group Mean % of Target 20.0 Gentamicin µg/ml Peer Group Mean % of Target 25.0 Imipramine ng/ml Peer Group Mean % of Target 25.0 AccuTest Evaluation Criteria V1.0 JANUARY 2008

7 Lidocaine mg/l Peer Group Mean SD 2 Lithium mmol/l Peer Group Mean Combination (max.limit) Methotrexate µmol/l Peer Group Mean % of Target 25.0 N-acetylprocainamide µg/ml Peer Group Mean % of Target 25.0 Nortriptyline ng/ml Peer Group Mean % of Target 25.0 Phenobarbital µg/ml Peer Group Mean % of Target 20.0 Phenytoin µg/ml Peer Group Mean % of Target 25.0 Primidone µg/ml Peer Group Mean % of Target 25.0 Procainamide µg/ml Peer Group Mean % of Target 25.0 Quinidine µg/ml Peer Group Mean % of Target 25.0 Salicylates mg/dl Peer Group Mean % of Target 25.0 Theophylline µg/ml Peer Group Mean % of Target 25.0 Tobramycin µg/ml Peer Group Mean % of Target 25.0 Tricyclic Antidepressants ng/ml Peer Group Mean % of Target 25.0 Valproic Acid µg/ml Peer Group Mean % of Target 25.0 Vancomycin µg/ml Peer Group Mean % of Target 25.0 AccuTest Evaluation Criteria V1.0 JANUARY 2008

8 Participation Statistics Laboratorio Hospital Nossa Senhora da Conceicao Results Deadline: 2009/Mar/17 number of test samples: 5 Evaluation Group: USA Page 3 of 3 Print Date: 2009/Mar/30 Analyte/Unit of Measure Sample A Sample B Sample C Sample D Sample E Comparison_Group # Mean SD CV(%) Range # Mean SD CV(%) Range # Mean SD CV(%) Range # Mean SD CV(%) Range # Mean SD CV(%) Range Red Blood Cell Count (1.0E12/L) - 5 Parts G Evaluation Criteria: Peer Group Mean ± 6% AR:All Results ME:Flow Cytometry SM:Electrical Impedance/Conductivity IM:Sysmex Red Cell Distribution Width (%) - 5 Parts G Evaluation Criteria: Peer Group Mean ± (10% or 3SD) AR:All Results ME:Calculated SM:RDW=S.D.of Red Cell Volume/MCV IM:Sysmex White Blood Cell Count (1.0E09/L) - 5 Parts G Evaluation Criteria: Peer Group Mean ± 15% AR:All Results ME:Flow Cytometry SM:Electrical Impedance/Conductivity IM:Sysmex # : Statistical Count Peer Group(PG) Codes: AR-all results; ME-method; SM-submethod; RM-reagent manufacturer; RG-reagent group; RE-reagent; IM-instrument manufacturer; IG-instrument group; ID-instrument model Support from AccuTest Member_ International EQA Collaboration support@digitalpt.com tel fax

9 SMILE Johns Hopkins University Baltimore, MD USA Appendix C - Example Appendix A - Hematology Manual Evaluation Template WBC - x10 9 /L +15% Your result Mean SD Lower Upper SDI Your Grade Your result Mean SD Lower Upper SDI Your Grade Acceptable #DIV/0! Unacceptable Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable CAP 2009 HEFG-1 Accutest Survey RBC - x10 12 /l +6% HGB-g/dL +7% HCT-% +6% Your result Mean SD Lower Upper SDI Your Grade Your result Mean SD Lower Upper SDI Your Grade #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable MCV-fl +3SD RDW +3SD Your result Mean SD Lower Upper SDI Your Grade Your result Mean SD Lower Upper SDI Your Grade #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable #DIV/0! Unacceptable Pro App A Hematology EQA Calculation Sheet-example.xls 20 March 2006

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