Food Lactase Lactazme-B is a neutral lactase (EC ) preparation derived from bacillus circulans.

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1 Enzyme For better life of humans and animals with new concept enzymes Feed enzyme Amylase Alkaline Protease Cellulase Xylanase Phytase New generation phytase source from seawater bacteria. High enzyme activity in the PH condition of monogastric animal's digestive tract. Lipase GF Calb-1, GF CalB-P and GF CalB-L are highly active preparations of lipase from Canadian Antartica. Food Lactase Lactazme-B is a neutral lactase (EC ) preparation derived from bacillus circulans. Chitosanase Chitosan is a natural product derived from chitin, a polysaccharide found in the exoskeleton of shellfish like shrimp or crabs and insects. it inhibits LDL-cholesterol and boosts HDL- cholesterol. In addition, it is known that it helps to control pressure and to prevent constipation. Chitosan oligosaccharide is obtained by an enzymatic hydrolysis of chitosan using chitosanase. Chitinase Industry Catalase Catalase is en enzyme that presents in the cells of plants, animals and aerobic (oxygen required) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and harmful oxidizing agent, into water and molecular oxygen.

2 Enzyme for feed Alkaline-Protease Enzyme Activity : Alkaline protease 2, unit/gram GenoPro-AK is produced by submerged fermentation from Bacillus licheniformis, which can hydrolyze protein into free amino acids. It is widely applied in casein-sodium, chondroitin, leather de-haring, detergent industry and feed industry. Product description and its storage Color : yellow to brown Appearance : powder (85% pass standard sieve 4) Water content: less than 8% Specific activity : 2, unit/g or more (One unit is defined to be the amount of the enzyme that decomposed 1 µmol of the tyrosine for 1 minute at 4 C, 1.5.) Odor : a slight fermentative odor Packaging : 1kg, 1kg, 25kg and bulk Reaction condition Optimal temperature is 4~5 C Optimal is 9~12 Recommended dosage Dosage 2~4g/ ton Feed GenoPhos (Phytase) PCT WO (phytase produced from citrobacter braakii) Enzyme activity : 5,unit/gram, 1,unit/gram, 2, unit/gram Innovative Technology - Protein Engineering High enzyme activity in the PH condition of monogastric animal s digestive tract. Strong resistance against digestive proteinase of monogastric animal s digestive tract. Feed Adjustable Formulation Particle size was optimized for maxibility and lowwability in feed manufacturing process. High Efficacy in Poultry and Pigs Egg quality (egg weight, Haugh unit, egg shell strength, etc.) in laying hens was increased Growth performance ( weight gain, FCR, etc.) in poultry and pigs was improved. Recommended Use Dosage can be determined by specific conditions such as feed compositions, processing, cost, etc. 5~2g/ton of complete feed available. Expiration date 12 months from manufacturing date. Packaging 2kg paper bag, double layer polyethylene bag. Recommended Use (based on 5,unit/gram) Species of livestock Recommended dosage (g/ton of feed) Phytase activity (unit/kg of feed) Laying hens 3 Broiler 5 Pigs 5 Others 5 Specification Formulation Powder type Granular type Coated type 5. IU/g Genophos* 5P Genophos* 5G Genophos* 5C 1. IU/g Genophos* P Genophos* G Genophos* C 2. IU/g Genophos* 2P Genophos* 2G Genophos* 2C Appearance Fine yellowish white powder Min. 95%(3mesh) Fine yellowish white granule Min. 95%(25-7mesh) Fine yellowish brown granule Min. 95%(25-7mesh)

3 Enzyme for feed Geno-Lipa (Lipase) Geno-Lipa is a lipase from candida antartica produced by fermentation of genetically modified Saccharomyces cerevisiae. Geno-Lipa is a triacylglycerol hydrolize (EC* ) and it has 1,3 positional specificity. Geno-Lipa is strongly bound to the carrier that used for immobilization is microporous ion exchange resin by adsorption. Activity Synthetic activity using oleic acid and butanol Lipase 1,2 ~ 5, unit/gram Opt. Temp. & Opt. Opt. Temp. Opt Storage and Expiration Store at dry and cool place 12 months from manufacturing date Before treatment of Lipase to Tallow 12hours after treatment of Lipase to Tallow BS BS Emulsion Emulsion PL Lipases Phospholipase PL Lipases Colipase Phospholipase Colipase Digestion (intestinal lumen) Digestion (intestinal lumen) Mixed micelle Mixed micelle AGPAT AGPAT PA PA MGAT DGAT MGAT DGAT MPT ACAT ACAT CE ApoB CE ApoB Absorption (enterocyte) Absorption (enterocyte) MPT Lymph Lymph

4 Enzyme for food Lactazyme-B (Lactase) Lactazme-B is a neutral lactase (EC ) preparation derived from Bacillus circulans. The enzyme catalyzes the hydrolysis of lactose and the galactosyl transfer reaction. In the galactosyl transfer reaction, it is advantageous to react at high temperature because of the low solubility of lactose. Product Properties Opt. Temp. : 5ºC Opt. : Temperature and activity of lactase from B.circulans (onpg method) Specification Lactazyme-B : 4, unit/gram (unit definition: one unit is defined as the amount of enzyme which will liberate 1u mol of per minute from ongp at 5degree Cel, 6.) Appearance : yellow~light brown, powder Application : GOS production, Dairy industry Storage Store under cool and dry condition

5 Enzyme for food Cooking oil CalB-IM (Lipase) GF CalB-Im is a lipase from candida antartica produced by fermentation of genetically modified Saccharomyces cerevisiae. Product Properties Product Type GF CalB-IM is a triacylglycerol hydrolize (EC* ) and it has 1,3 positional specificity. CalB-IM is strongly bound to the carrier that used for immobilization is microporous ion exchange resin by adsorption. Activity Synthetic activity using oleic acid and butanol GF CalB-IM : 1,2 unit/gram Opt. Temp. & Opt. Opt. Temp. Opt Storage Store at dry and cool place ( 2 ~ 8 C) GF Chitosanase Product Properties Bind excess fat and inhibit fat absorption Lower LDL/boost HDL cholesterol Anti-cancer, anti bacterial agent Support immunity-lower blood sugar Control blood pressure Prevent constipation Enhance absorption of calcium Prevent heart disease Specification Origin : Bacillus species Endo type enzyme, producing mainly 3-6 oligosaccharides Molecular weight : 46, dalton Optimum temperature : degree celcius Optimum PH : 7. Appearance : Light yellow~white powder Use : Production of chito-oligosaccharides 1g chitosanase/1kg chitosan, 24hr reatcion at 37degree celcius Specific activity : 36. units/g Package : 1g,5g,1g,5g,g,5g,1kg and bulk Chitosan is a natural product derived from chitin, a polysaccharide found in the exoskeleton of shellfish like shrimp or crabs and insects. It inhibits LDL-cholesterol and boosts HDL-cholesterol. In addition, it is known that it helps to control pressure and to prevent constipation. Chitosan oligosaccharide is obtained by an enzymatic hydrolysis of chitosan using chitosanase. Chitosan oligosaccharide is a mixture of oligomers od D-glucosamine and its molecular weight is less than 2.5 dalton. Chitosan oligosaccharide is absorbed very well in the body and its function is very high compared with chitosan. Temperature & Optimum temp Product Profile TLC Reaction condition 1,u chitosanase / 1kg chitosan 4 C, 24h reaction Substrate MW 2, Chitosan Chitosan monomer dimer trimer tetramer pentamer hexmer M Substrate MW 13, Chitosan Chitosan monomer dimer trimer tetramer pentamer hexmer M M : marker 1: h 2 : 4h 3 : 16h 4 : 24h HPLC COS Profile COS No

6 Enzyme for Industry GFzyme Katalase Catalase is an enzyme that presents in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and harmful oxidizing agent, into water and molecular oxygen. It is widely used for removal of H22 in textile industry and semiconductor. it saves time and energy and is environmental-friendly. 2H2O2 2H2O + O2 H2O2 + RH2 2H2O + R Recommendation of Operational Condition Stable range : 4. ~ 11. Optimum : 6. ~ 8. Temperature stability : stable at 7 C for one hour Optimum temperature : 35 ~ 55 C Process time : 5 ~ 2 minutes Dosage :.1 ~.5 g/l Guideline for the Bleach Clean-up Drain the bleaching liquid Fill the bath with suitable amount of fresh water Adjust and temperature for using enzyme Add.1~.5 g/l of GFzyme-KatalaseTM After 5-2 minutes, continue dyeing procedure in the same bath (Generally no need to change water, but if needed, change water for elimination of impurities) Comparison of the Process Rinsing with water Bleaching Rinsing Rinsing Rinsing Rinsing Dyeing Using GFzyme-Katalase TM Bleaching Rinsing Katalase TM & Dyeing Package and Storage Package unit : 2kg, 22kg drum and 1 ton bulk Storage : avoid a direct ray of light and keep cool Stability test for Temperature and 4 2 A B GF 4 2 A B GF Time(min) Heat Stability at 7 C Stability (after 1 hour) Biogenoci Co., Ltd. in Korea (3F, Dongjin B/D) 45, Nonhyeon-ro 26 Gil, Gangnam-gu, Seoul, Korea, Tel : Fax : dhlee@biogenicgroup.com

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