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1 57-63 /387 / / *** ** * - ** - * - - *** /9/23 : 87/3/22 : :. :. :... 8/5±0/5 :. 00 2/±0/2 5/5±0/68 8/26±0/67 5/2±/02 7/6±0/69 58/9±/95 56/0±4/4 (SFAs) 24/8±0/85 (MUFAs) /±/06 65/9±3/39 22/6±/46 8/8±0/7 6/9±/45 (PUFAs). 00 4/0±0/96 0/6±0/35 :.. : (MUFAs) (TFAs) (PUFAs) TFAs SFAs. LDL-C.() LDL-C - HDL-C TFAs. - :. -. (SFAs) : : sasgari@yahoo.com 57
2 .(7) LDL-C.(89) ) (. : ( ) ( ). (2 ) (2 ( ) ). (American oil chemists society) AOCS (20) 20. 0/5 NaOH-Methanol 7 2. mol/l 3. 00ºC BF3-Methanol 00 ºC 5 4 n- 2. NaCl 7 Hexane. ( ). ) LDL-C (6- (c-reactive protein) CRP HDL-C (2) a HDL-C LDL-C (3) TFAs.(546) -.(7-0) (Food and drag andministration) FDA TFAs.() TFAs.(32) TFAs TFAs (%70 ) ( ).(2) MUFA.(4).(65) %59 ) SFAs.( (C8:0) 58
3 387 / / 56/0±4/4 24/8±/85 6/9±/45 MUFAs PUFAs 58/9±/95 22/6±/ /8±0/ /26±0/67 8/5±0/85. MUFAs 67/±/06 65/9±3/ /6±3/39 PUFAs. SFAs 00 4/0±0/ /2±/02 7/6±0/69 SAFs /±0/2 5/5±0/68. (MUFA+PUFA) :SFA PUFA:SFA (P:S). (2) 0/6 P:S 0/92. 0/5 0/ 4/35 (m+p):s 5/3 0/53 0/57.( ) n-hexane 00 (GC).(20) GC Acme 6000 MGC TR-CN00 0/ / Oven 250ºC 240ºC ºC 200ºC 5 50ºC ºC ºC 20 99/99. ) 30. ( 30.. ( (2 : ) ) 59
4 . 00 : 0 /4± 0 / 0 /7± 0 / 05 0 /6± 0 / 70 4 /8± 0 / 70 4 /2± 0 / 45 6 /5± 2 / 57 0 /± 0 / 4 2 /3± 0 / 5 3 /9± 0 / 6 0 /± 0 / 04 5 /2± / /± / 06 4 /0± 0 / 96 0/2±0/ /3 ± 0/50 /4±0/7 6/±0/3 64/4±4/6 /±0/3 9/7±0/52 4/4±0/43 0/9±0/2 0/±0/02 7/6±0/69 65/9±3/39 0/6±0/35 /3±0/4 /±0/36 0/4±0/3 8/0±0/56 3/6±0/7 0/2±0/ 0/±0/06 25/3±2/05 0/±0/04 0/6±0/2 7/5±0/4 22/0±0/73 8/5±0/53 5/0±0/26 0/8±0/ 3/8±0/26 0/3±0/ 58/9±/95 22/6±/46 8/8±0/7 2/±0/36 4/2±0/64 2/0±0/36 4/5±0/72 8/9±/5 2/0±0/45 3/2±0/45 2/4±0/98 /6±0/50.4 0/5±0/3 9/±/30 20/0±0/95 7/5±0/6 3/±0/82 /0±0/3 3/8±0/65 0/±0/05 /2±0/6 56/0±4/4 24/8±/85 6/9±/45 C6:0(Caproic acid) C8:0(Caprylic acid) C0:0(Capric acid) C2:0(Lauric acid) C4:0(Myristic acid) C4: cis(myristoleic acid) C5:0(Pentadecanoic acid) C6:0(Palmitic acid) C6: cis(palmitoleic acid) C6: trans (Palmitelaidic acid) C7:0(Heptadecanoic acid) C8:0(Stearic acid) C8: 9-cis(Oleic acid) C8: 9-trans(Elaidic acid) C8:2(Linoleic acid) C8:2 6-trans(Trans-6- linoleic acid) C8:3(Linolenic acid) C20:0(Arachidic acid) C20:(Eicosenoic acid) Total SFA Total MUFA Total PUFA 2 /± 0 / 2 5/5±0/68 8/26±0/67 8/5±0/85 Total TFA 0 /92± 0 / 07 0/6±0/04 0/5±0/04 0/±0/02 P:S 5 /2± 0 / 23 4/35±0/ 0/53±0/08 0/57±0/03 (m+p):s SFA: Saturated fatty acid-mufa:monounsaturated fatty acids- PUFA: Polyunsaturated fatty acids-tfa: Trans fatty acid-p:s:pufa:sfa; (m+p):s, (MUFA+PUFA) :SFA. "± ". : 60
5 387 / /.(28).(29) P:S (m+p):s P:S.(30) (m+p):s. FDA (20)..(3). : ). : ( 855. LDL-C.(22) LDL-C. LDL-C LDL-C (2398). ( ).(24).(4).(25).(26)..(27) 59 (C2:0) (C6:0) (C4:0).(7). - 6
6 :. Matthan NR, Welty FK, Barrett PH, Harausz C, Dolnikowski GG, Parks JS, et al. Dietary hydrogenated fat increases high-density lipoprotein apoa-i catabolism and decreases lowdensity lipoprotein apob-00 catabolism in hypercholesterolemic women. Arterioscler Thromb Vasc Biol Jun; 24(6): Mensink RP, Zock PL, Kester AD, Katan MB. Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. Am J Clin Nutr May; 77(5): Dashti N, Feng Q, Freeman MR, Gandhi M, Franklin FA. Trans polyunsaturated fatty acids have more adverse effects than saturated fatty acids on the concentration and composition of lipoproteins secreted by human hepatoma HepG2 cells. J Nutr Sep; 32(9): Willett WC. Trans fatty acids and cardiovascular disease-epidemiological data. Atheroscler Suppl May; 7(2): Mensink RP, Katan MB. Effect of dietary trans fatty acids on high-density and low-density lipoprotein cholesterol levels in healthy subjects. N Engl J Med. 990 Aug; 323(7): Olendzki B, Speed C, Domino FJ. Disease. Nutritional assessment and counseling for prevention and treatment of cardiovascular. Am Fam Physician Jan; 73(2): Lopez-Garcia E, Schulze MB, Meigs JB, Manson JE, Rifai N, Stampfer MJ, et al. Consumption of trans fatty acids is related to plasma biomarkers of inflammation and endothelial dysfunction. J Nutr Mar; 35(3): Moens AL, Goovaerts I, Claeys MJ, Vrints CJ. Flow-mediated vasodilation: a diagnostic instrument, or an experimental tool? Chest Jun; 27(6): Slattery ML, Benson J, Ma KN, Schaffer D, Potter JD. Trans-fatty acids and colon cancer. Nutr Cancer. 200; 39(2): Mozaffarian D, Katan MB, Ascherio A, Stampfer MJ, Willett WC. Trans fatty acids and cardiovascular disease. N Engl J Med Apr; 354(5): FDA (Food and Drug Administration, Center for food safety and applied nutrition). Trans fat now with saturated fat and cholesterol on nutrition facts panel, De La Torre A, Gruffat D, Durand D, Micol D, Peyron A, Scislowski V, et al. Factors influencing proportion and composition of CLA in beef. Meat Sci. 2006; 73(2): Giugliano D, Ceriello A, Esposito K. The effects of diet on inflammation: emphasis on the metabolic syndrome. J Am Coll Cardiol Aug; 48(4): Owen RW, Giacosa A, Hull WE, Haubner R, Spiegelhalder B, Bartsch H. The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. Eur J Cancer Jun; 36(0): Ghafoorunissa G. Role of trans fatty acids in health and challenges to their reduction in Indian foods. Asia Pac J Clin Nutr. 2008; 7(Suppl ): Achaya KT. Fat status of Indians-a review. J Sci Ind Res. 987; 46(3): Fernandez ML, West KL. Mechanisms by which dietary fatty acids modulate plasma lipids. J Nutr Sep; 35(9): Parthasarathy S, Khoo JC, Miller E, Barnett J, Witztum JL, Steinberg D. Low density lipoprotein rich in oleic acid is protected against oxidative modification: implications for dietary prevention of atherosclerosis. Proc Natl Acad Sci USA May; 87(0): Noseda G. Fats and oils (including omega3, omega6). Ther Umsch Sep; 62(9):
7 387 / / 20. AOCS Official Method Ce. Preparations of methyl esters of fatty acids. 997; Vorbeck,ML, Mattick LR, Lee FA, Pederson CS. Preparation of Methyl Esters of Fatty Acids for Gas-Liquid Chromatography. Quantitative Comparison of Methylation Techniques.Anal. Chem. 96; 33(): Hodson L, Skeaff CM, Chisholm WA. The effect of replacing dietary saturated fat with polyunsaturated or monounsaturated fat on plasma lipids in free-living young adults. Eur J Clin Nutr. 200 Oct; 55(0): Kromhout D, Menotti A, Kesteloot H, Sans S. Prevention of coronary heart disease by diet and lifestyle: evidence from prospective cross-cultural, cohort, and intervention studies. Circulation Feb; 05(7): Grundy SM. The role of cholesterol management in coronary disease risk reduction in elderly patients. Endocrinol Metab Clin North Am. 998 Sep; 27(3): Serra-Majem L, de la Cruz JN, Ribas L, Salleras L. Mediterranean diet and health: is all the secret in olive oil? Pathophysiol Haemost Thromb Sep-2004 Dec; 33(5-6): Lambelet P, Grandgirard A, Gregoire S, Juaneda P, Sebedio JL, Bertoli C. Formation of modified fatty acids and oxyphytosterols during refining of low erucic acid rapeseed oil. J Agric Food Chem Jul; 5(5): Martin CA, Milinsk MC, Visentainer JV, Matsushita M, de-souza NE. Trans fatty acidforming processes in foods: a review. An Acad Bras Cienc Jun; 79(2): Berner LA. Roundtable discussion on milk fat, dairy foods, and coronary heart disease risk. J Nutr. 993 Jun; 23(6): Elson CE. Tropical oils: nutritional and scientific issues. Critical Reviews. Food Sci. Nutr. 992; 3: Hajri T, Khosla P, Pronczuk A, Hayes KC. Myristic acid-rich fat raises plasma LDL by stimulating LDL production without affecting fractional clearance in gerbils fed a cholesterolfree diet. J Nutr. 998 Mar; 28(3): Rawashdeh AYA. Influences of olive oil and ghee (samen balady) on serum cholesterol of Jordanians. Paki J Nutr. 2002; (6): Gebauer SK, Psota TL, Kris-Etherton PM. The diversity of health effects of individual trans fatty acid isomers. Lipids Sep; 42(9):
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