Diphasic Effects of Astragalus membranaceus BUNGE (Leguminosae) on Vascular Tone in Rat Thoracic Aorta
|
|
- Vivien Merryl Rice
- 5 years ago
- Views:
Transcription
1 1450 Biol. Pharm. Bull. 28(8) (2005) Vol. 28, No. 8 Diphasic Effects of Astragalus membranaceus BUNGE (Leguminosae) on Vascular Tone in Rat Thoracic Aorta Bi-Qi ZHANG, a Shen-Jiang HU,*,a,d Li-Hong QIU, a Qi-Xian SHAN, b Jian SUN, a Qiang XIA, b and Ka BIAN c,d a Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University; No. 79, Qingchun St, Hangzhou , Zhejiang, P.R. China: b Department of Physiology, College of Medicine, Zhejiang University; No. 353, Yan an St, Hangzhou , Zhejiang, P.R. China: c Department of Integrative Biology and Pharmacology, The University of Texas-Houston Medical School; 6431 Fannin, Houston, Texas, 77030, U.S.A.: and d E-Institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine; No. 1200, Chalun St, Shanghai , P.R. China. Received March 22, 2005; accepted April 28, 2005 This study was designed to investigate the effects of the aqueous ethanol extract of Astragalus membranaceus BUNGE (Leguminosae) on rat thoracic aorta. Isometric tension was recorded in response to drugs in organ bath. In endothelium-intact aortic rings, A. membranaceus extract induced a significant dose-dependent relaxation of the rings precontracted by phenylephrine, which could be inhibited by preincubation with L-N(w)-nitro-arginine methyl ester or methylthioninium chloride. In endothelium-denuded ones, the extract could dose-dependently relax the rings contracted by phenylephrine, not by KCl; and it could also attenuate contractile response to phenylephrine, not to caffeine or phorbol-12,13-diacetate in Ca 2 -free medium; but it failed to affect the CaCl 2 - induced enhancement of contractile response to phenylephrine in Ca 2 -free medium. These results indicate that nitric oxide signaling and Ca 2 -handling pathway are involved in the A. membranaceus extract-induced vasodilatation. Key words Astragalus membranaceus; nitric oxide; calcium ion; thoracic aorta; vasomotion The roots of Astragalus membranaceus BUNGE (Leguminosae) are amongst the most popular and important Qi (pronounce chee) tonifying adaptogenic herbs in China, their use dates back more than 2000 years, and are recorded in Shen Nong s Materia Medica. According to ethnobotancical data collected in China, A. membranaceus has been prescribed for centuries for diarrhea, frequent colds, spontaneous sweats, fatigue, and edema. Furthermore, A. membranaceus is alleged to possess the effects of antioxidative damage, 1) immune-stimulation, 2) and antiviral infection. 3) Recently, it has received more attention that the fact recorded in ancient Chinese texts that A. membranaceus possessed an antihypertensive effect by raising the yang qi of the spleen and kidney, since deficiency qi formed by the disorder of spleen and kidney is considered to be the basic pathogeneses of hypertension. 4) A great deal of studies demonstrated that A. membranaceus was beneficial on the process of hypertension in animals and humans, 5 7) and a cooperative mechanism of renin angiotensin aldosterone system, kallikrein bradykinin system, and central neuropeptide 8) was used to explain this effect of A. membranaceus. We also found in the past experiments that the administration of A. membranaceus could attenuate the progression of blood pressure in spontaneously hypertensive rats concomitant with restoration of baroreflex sensitivity and reduction of Ca 2 concentration in lymphocyte and vascular smooth muscle cells (VSMC). 9 11) Additionally, in vivo experiment had demonstrated that A. membranaceus could induce the vasodilatation. 12) However, there is no experiment evidence available to show the exact mechanism of vasorelaxation produced by A. membranaceus. The present study is conducted to elucidate various possible mechanisms in vasomotor response of A. membranaceus. MATERIALS AND METHODS Plant Material and Preparation of the Extracts A. membranaceus roots were collected in the northern region of Gansu Province, China, in the autumn (2001) and identified by the Quality Assessment of Di ao Group, Sichuang, China. And a voucher specimen was deposited in the Academic Department of Di ao Group (STQ-C-00203). A. membranaceus roots were air dried. A crude extract was prepared by decoction of g in 500 ml water for 3 times (90 min per time). The obtained extract was combined, filtered and concentrated. Then all the aqueous extract was extracted with 400 ml ethanol (75% and 85%) twice. After the ethanol was retrieved, the active fractions were recovered, filtered and lyophilized (yield 0.28%). Consequently, the final extract was used in the study after dissolution in distilled water at 37 C and stirred for 60 min. Chemicals Phenylephrine (PE), acetylcholine (ACh), L- N(w)-nitro-arginine methyl ester (L-NAME), methylthioninium chloride (MC), phosphoramidon, ethylene glycol-bis [b-aminoethyl ether]-n,n,n,n -tetraacetic acid (EGTA), caffeine and phorbol-12,13-diacetate (PD) were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). They were dissolved in distilled water and diluted with Krebs Henseleit (K H) solution before use. All chemicals were of the highest purity available. Experiment Animals The investigation conformed to the Chinese Regulation for Administration of Laboratory Animal (SSTC 1988/2). Health male Sprague Dawley rats weighing g (Laboratory Animal Center of Zhejiang College of Traditional Chinese Medicine, Hangzhou, China) were housed in conventional cages with free access to water and rodent chow at the controlled temperature and humidity with a 12-h light/dark cycle. Preparation of Thoracic Arterial Rings from Sprague To whom correspondence should be addressed. s0hu0001@hotmail.com 2005 Pharmaceutical Society of Japan
2 August Dawley Rats Rats were euthanized by decapitation using small animal guillotine. The thoracic aorta was immediately isolated and immersed in oxygenated K H solution 4 C of the following composition (in mm): NaCl 118.3; KCl 4.7; MgSO 4 1.2; KH 2 PO 4 1.2; NaHCO ; CaCl 2 2.5; Glucose Then the adherent connective tissue was cleaned and blood vessel was cut into 3 4 mm rings, with the special care of avoiding damage of endothelium. In some preparations, endothelium was mechanically removed by gently rubbing the lumen. The rings were mounted horizontally between two stirrups in organ bath filled with 10 ml K H solution at 37 C, ventilated continuously with 95% O 2 and 5% CO 2. The isometric tension was recorded with a force transducer (JZ101, metrical range: g) and MedLab 5.0v recording system (Nanjing Medease Science and Technology Co. Ltd). Experimental Procedure of Isometric Tension Rings were equilibrated for 60 min at 2.0 g resting tension, and then challenged with KCl ( M) at least 3 times until a reproducible maximal contractile response was obtained. After a further equilibration period of 30 min, the integrity of the endothelium was assessed in all preparations by determining the ability of ACh ( M) to induce more than 80% relaxation of rings pre-contracted with PE ( M). The endothelium was considered to be removed when there was less than 10% relaxation response to ACh. Protocol 1: Effects of A. membranaceus on Vascular Tone: The first series of the experiments were conducted to assess endothelium-dependent or independent effects of A. membranaceus on isolated aortic rings. When the tension was at resting state or reached a plateau induced by PE ( M), A. membranaceus ( g/l) was cumulatively added into the organ bath with 10 min interval. The rings with intact or denuded endothelium were always tested in parallel. Protocol 2: Endothelium-Independent Vasomotor Response by A. membranaceus: The second series of the experiments were designed to determine the underlying mechanisms of A. membranaceus-induced vasomotor response in aortic rings without endothelium. In contrast to the response of A. membranaceus ( g/l) to PE, KCl ( M) was also employed to constrict the denuded aortic rings. A plateau contractile response to KCl or PE was also obtained as a time control. Additionally, after the preparations were serially washed and equilibrated for 60 min by Ca 2 -free medium (mm: NaCl 118.3, KCl 4.7, KH 2 PO 4 1.2, MgSO 4 1.2, NaHCO 3 25, Glucose 11.0, EGTA 0.05), PE ( M), PD ( M) (an activator of protein kinase C, PKC), 13) or caffeine ( M) (an activator of ryanodine receptor) 14) was used to contract the denuded rings. In order to explore the probable effect of extracellular Ca 2 inflow, 2.5 mm CaCl 2 was added after PE stimulated the rings. Then the effect of A. membranaceus ( g/l) was tested. A time control (same protocol as above except for the omission of A. membranaceus to the second application of PE, PD or caffeine) was always run in parallel. Following the initial control response in Ca 2 -free medium and before the second test contraction (to PE or caffeine), a M KCl-induced contraction in K H solution was obtained to refill the Ca 2 stores in the vascular tissues. Protocol 3: Endothelium-Dependent Vasomotor Response by A. membranaceus: In the third series of experiments, the potential influence of A. membranaceus on PE-induced vasomotor function in the intact rings was examined. For pretreatment, endothelium-intact rings were incubated with L- NAME ( M) (a specific inhibitor of nitric oxide synthase), MC ( M) (an inhibitor of guanylate cyclase) for 15 min 15) and phosphoramidon ( M) (an inhibitor of endothelin converting enzyme) for 20 min 16) before administration of PE. Then the response curves of A. membranaceus were recorded. The rings with or without the treatment of inhibitors were always tested in parallel. Statistical Analysis Relaxant responses were expressed as the percentage decreases of the magnitude of the contraction induced by PE or KCl ( 100%) before the application of vasodilators, and the contractile responses as the ratio of the responses following and before the application of A. membranaceus (second response/first response). All results are expressed as mean S.E.M. Statistical analysis was performed with t-test or ANOVA followed by Newman Keuls test. Differences were accepted as statistically significant at p values 0.05 (GraphPad Prism). RESULTS PE ( M) induced a similar sustained contraction of aortic rings in each group with a peak tension of about g in intact aortic rings and g in denuded ones. Effects of A. membranaceus on the Vasotension All doses ( g/l) of A. membranaceus exhibited negligible vasomotor actions on aortic rings with or without endothelium at resting tension (Fig. 1). A. membranaceus ( g/l) produced dose-dependent relaxation in endothelium-denuded rings (Fig. 2), after the plateau tension induced by PE. However, in PE-precontracted endothelium-intact rings, low doses ( g/l) of A. membranaceus showed a more significant relaxation than that in denuded ones (Fig. 2), while high dose ( g/l) of the extract induced a transient contraction (Figs. 2, 3). Endothelium-Independent Vasodilatation by A. membranaceus In contrast to the effect on PE-contracted endothelium-denuded rings in K H solution, A. membranaceus ( g/l) had no dilatation-effect on rings stimulated by KCl ( M) (Fig. 4). And in the experiment with Ca 2 -free medium, aortic contraction elicited by PE, caffeine or PD was decreased substantially, as these vascular contractions in Ca 2 -free medium are mediated only via the release of Ca 2 from the intracellular stores or phosphorylation of myosin light chain. A. membranaceus ( g/l) could inhibit the contraction induced by PE ( M), but not block the extracellular Ca 2 inflow, because 2.5 mm CaCl 2 induced a similar enhancement of contraction by PE, compared with the control ( % vs %, p 0.05). Furthermore, A. membranaceus ( g/l) failed to induce an attenuation of contractile response by PD ( M) and caffeine ( M) (Fig. 5). Endothelium-Dependent Vasomotor Effects by A. membranaceus Pre-incubation of the intact rings with L-
3 1452 Vol. 28, No. 8 Fig. 1. Effects of A. membranaceus on the Vasomotion of Aorta Rings in the Resting Tension A. membranaceus ( g/l) was cumulatively added into the bathing solution in 10 min interval during the resting phase (2.0 g) in (A) endothelium intact or (B) removed rings. Values of each group are expressed as mean S.E.M. of 8 experiments. Fig. 2. Vasorelaxant Effects of A. membranaceus ( g/l) on Phenylephrine ( M)-Induced Contractions in Aortic Rings (A) with or (B) without Endothelium Values of both groups are expressed as mean S.E.M. of 8 experiments. Contractile responses are expressed as the percentage from maximal contraction elicited by phenylephrine ( M). p 0.05, p 0.01, compared with the control group. Fig. 3. Transient Vasocontractile Response of A. membranaceus (Closed Circle) against Phenylephrine-Induced Endothelium-Intact Aortic Rings With (A) or (B) without the incubation of phosphoramidon ( M) for 20 min before phenylephrine ( M) was added, the effect of A. membranaceus ( , and g/l, showed by a, b, c point) was obtained. For it was similar with Fig. 2, the effect of A. membranaceus ( g/l) was cut. Values of both groups are expressed as mean S.E.M. of 8 experiments. Contractile responses are expressed as the percentage from maximal contraction elicited by phenylephrine ( M). p 0.05, p 0.01, compared with corresponding values before administration. NAME ( M) markedly, incompletely inhibited A. membranaceus ( g/l)-induced relaxation from 36.0 to 7.3% (Fig. 6). Similarly, MC ( M) also showed an inhibition of endothelium-dependent vasodilatation from 36.0 to 8.0% (Fig. 6). Thus, NO signaling might play a major, but not full role in the vasodilatation effect of A. membranaceus. When the dose of A. membranaceus in the organ bath was stepwise raised to g/l, a transient contraction could be observed in endothelium-intact rings. Pretreatment of endothelium-intact rings with phosphoramidon ( M) significantly attenuated the transient contraction response (Fig. 3). DISCUSSION The present study first and detailedly explored the in vitro vascular effects of A. membranaceus in aortic rings isolated from rats. In the course of the study, a number of novel observations had been made, which contribute to better scien-
4 August Fig. 4. Vasorelaxant Effects of A. membranaceus ( g/l) on KCl ( M)-Induced Contractions in Aortic Rings without Endothelium Values of both groups are expressed as mean S.E.M. of 8 experiments. Contractile responses are expressed as the percentage from maximal contraction elicited by KCl ( M). Fig. 5. Inhibitory Effect of A. membranaceus ( g/l) on Phenylephrine ( M)-, Phorbol-12,13-diacetate ( M) and Caffeine ( M)-Induced Contractions in Ca 2 -Free Medium in Endothelium- Denuded Rings Each column with a bar represents mean S.E.M. of 8 experiments. Data are expressed as the percentage of the test response ratio (after/before A. membranaceus) with respect to the time control response ratios. p 0.01, compared with value in the corresponding vehicle group. Fig. 6. Vasorelaxant Effects of A. membranaceus ( g/l) on Phenylephrine ( M)-Induced Contraction in Presence of L-N(w)- Nitro-arginine Methyl Ester (L-NAME) ( M) and Methylthioninium Chloride (MC) ( M) in K H Solution in Endothelium-Intact Rings In the untreatment group, A. membranaceus or vehicle group was not pretreated with L-NAME or MC. Each column with a bar represents mean S.E.M. of 8 experiments. Contractile responses are expressed as the percentage from maximal contraction elicited by phenylephrine ( M). p 0.05, p 0.01, compared with value of the corresponding vehicle group. p 0.01, compared with value of the experiment subgroup in the untreatment group. tific understanding of their therapeutic use for antihypertension associated with Qi deficiency caused by the disorder of spleen and kidney in China. First, although neurohumoral factors may contribute to antihypertension for the crude extract of A. membranaceus observed previously, 8) it is apparent that direct endothelium-dependent and independent vasorelaxation should be prominently taken into account according to this study. Second, A. membranaceus dose-dependently dilated endothelium-denuded rings contracted with PE. However, the agent had no effect on the vessel contracted by KCl. The cellular mechanism of contraction involved in the response to KCl and PE is different. KCl induce Ca 2 influx via voltagedependent Ca 2 channel, which further activate Ca 2 -induced Ca 2 release through ryanodine-receptor. PE increase intracellular Ca 2 concentration with two mechanisms, 1) through receptor-gated Ca 2 channels; 2) mobilizes Ca 2 from intracellular stores via the inositol-1,4,5-trisphosphate (IP 3 ) receptor or induces secondly myosin light chain phosphorylation via activating PKC. 17,18) That is, the vasorelaxant action of A. membranaceus seems to occur in a receptor-dependent manner in SMVC of rat aortas. However, the result that A. membranaceus failed to inhibit the contraction caused by addition of CaCl 2 in Ca 2 free solution was probably only due to its effects on the intracellular pathways. This hypothesis was confirmed in the further experiments that the contractions induced by PD and caffeine in Ca 2 -free medium were not affected by A. membranaceus equally. Thus, it appears that the IP 3 -induced Ca 2 release channels might be the site of action for A. membranaceus on endothelium-removed aortas. Third, NO is a potent vasodilator synthesized in the endothelium 19) by NO synthase, and causes VSMC relaxation through the activation of soluble guanylate cyclase. 20) The present study demonstrated that the extract of A. membranaceus dose-dependently inhibited the contraction induced by PE in intact aorta isolated from rats. This vasorelaxant action was mostly inhibited by treatment with L- NAME or MC. Endothelium-dependent relaxation of A. membranaceus seemed to be associated with NO signaling via guanylate cyclase activation since both L-NAME and MC could attenuate this response. This result was supported by other studies which had shown that A. membranaceus could increase the plasma level of NO 21) and induce the release of NO from VSMC in vitro by NO-soluble guanylate cyclasecyclic guanosine monophosphate pathway. 22) Finally, it was also observed that high dose ( g/l) A. membranaceus induced a transient contraction in arterial rings pre-constricted by PE. This result was identical with that obtained in another report where treatment with high dose of A. membranaceus reversibly increased blood pressure of patients without hypertension previously. 23) The mechanism underlying is under investigation. Blockage of the contraction by endothelin-converting enzyme phosphoramidon may suggest the involvement of an endothelin receptor mediated signaling. In summary, the findings indicated that A. membranaceus have diphasic effects of relaxation and contraction on the aorta rings. The mechanisms of relaxation may include inhibition of intracellular Ca 2 release in vascular smooth muscle cells, and endothelium dependent relaxation mediated by
5 1454 Vol. 28, No. 8 the NO-GC pathway, whilst the effect of transient contraction of A. membranaceus at a high dose was related with endothelin release. Acknowledgements This work was supported by grants from Province Administration of Traditional Chinese Medicine, Zhejiang, P.R. China (Project Number: 2000C54 and 2003C078); Foundation for Returnee from Personal Department of Zhejiang Province, P.R. China (Project Number: ZRZ ); and by E-Institutes of Shanghai Municipal Education Commission, P.R. China (Project Number: E ). The authors are grateful to Prof. Wenquan Liang of the Department of Pharmacology, Zhejiang University, for his technical help. REFERENCES 1) Toda S., Shirataki Y., J. Ethnopharmacol., 68, (1999). 2) Jiao Y., Wen J., Yu X., Chin. J. Integrated Tradit. Chin. West. Med., 19, (1999). 3) Huang Z. Q., Qin N. P., Ye W., Chin. J. Integrated Tradit. Chin. West. Med., 15, (1995). 4) Gu Y., Deng D. M., Chin. Arch. Tradit. Chin. Med., 19, (2001). 5) Jia S. Q., Tianjin Pharm., 6, (1994). 6) Zhao C. L., Tradit. Chin. Med. Res., 14, (1998). 7) Zha Y. Z., J. Tradit. Chin. Med., 41, 329 (2000). 8) Song D. J., Gu D. G., Mao S. Y., Chin. Tradit. Herb. Drugs, 20, (1989). 9) Chen Z. K., Hu S. J., Sun J., Xia Q., Shen Y. L., Zheng X., Chin. J. Lab. Diagn., 7, (2003). 10) Chen Z. K., Hu S. J., Sun J., Xia Q., Shen Y. L., Tradit. Chin. Drug Res. & Clin. Pharmacol., 14, (2003). 11) Chen Z. K., Hu S. J., Zheng X., Wang G. B., Sun J., Xia Q., Shen Y. L., Chin. J. Chin. Mater. Med., 28, (2003). 12) Guo Z. G., Xue S. W., J. Tradit. Chin. Med., 21, (1980). 13) Ko W. H., Yao X. Q., Lau C. W., Law W. I., Chen Z. Y., Kwok W., Ho K., Huang Y., Eur. J. Pharmacol., 399, (2000). 14) Chew D. K., Orshal J. M., Khalil R. A., Hypertension, 42, (2003). 15) Huang Y., Life Sci., 60, (1997). 16) Zimmermann M., Jung C. S., Vatter H., Raabe A., Seifert V., Acta Neurochir. (Wien), 144, (2002). 17) Karaki H., Ozaki H., Hori M., Mitsui-Saito M., Amano K., Harada K., Miyamoto S., Nakazawa H., Won C. J., Sato K., Pharmacol. Rev., 49, (1997). 18) Kobayashi S., Gong M. C., Somlyo A. V., Somlyo A. P., Am. J. Physiol., 260, C (1991). 19) Moncada S., Palmer R. M., Higgs E. A., Pharmacol. Rev., 43, (1991). 20) Martin E., Davis K., Bian K., Lee Y. C., Murad F., Semin. Perinatol., 24, 2 6 (2000). 21) He L., Wang R. G., You Z. L., Yang Z. W., Chen X. D., Li J., Zhan X. L., Wang J., Acad. J. Hunan Tradit. Chin. Med., 23, 4 6 (2003). 22) Wang K. H., Foreign Med. Sci.: TCM Section, 18, (1996). 23) Wang C. H., Zhang J. J., Shandong J. Tradit. Chin. Med., 15, 351 (1996).
Relaxation responses of aortic rings from salt-loaded high calcium fed rats to potassium chloride, calcium chloride and magnesium sulphate
Pathophysiology 4 (1998) 275 280 Relaxation responses of aortic rings from salt-loaded high calcium fed rats to potassium chloride, calcium chloride and magnesium sulphate B.J. Adegunloye, O.A. Sofola
More informationMechanisms of simvastatin-induced vasodilatation of rat superior mesenteric arteries
BIOMEDICAL REPORTS 5: 491-496, 2016 Mechanisms of simvastatin-induced vasodilatation of rat superior mesenteric arteries YULONG CHEN 1,2*, HONGMEI ZHANG 3*, HUANHUAN LIU 2 and AILAN CAO 1,4 1 Shaanxi Pharmaceutical
More informationCalcium-dependent mechanisms mediate the vasorelaxant effects of Tridax procumbens
DOI 1.1515/jbcpp-213-3 J Basic Clin Physiol Pharmacol 214; 25(2): 161 166 Hussein M. Salahdeen, Gbolahan O. Idowu, Omoniyi K. Yemitan, Babatunde A. Murtala and Abdul-Rasak A. Alada Calcium-dependent mechanisms
More informationHawthorn Extract - Viable Treatment for Cardiovascular Disease or Unscrupulous Herbal Supplement?
Grand Valley State University ScholarWorks@GVSU Student Summer Scholars Undergraduate Research and Creative Practice 2010 Hawthorn Extract - Viable Treatment for Cardiovascular Disease or Unscrupulous
More informationDifferential responses to endothelial dependent relaxation of the thoracic and abdominal aorta from male Sprague-Dawley rats
Niger. J. Physiol. Sci. 27(December 12) 117 122 www.njps.com.ng Differential responses to endothelial dependent relaxation of the thoracic and abdominal aorta from male Sprague-Dawley rats 1 Oloyo, Ahmed
More informationEffects of Total Alkaloids in Buxus microphylla Leaves on Aorta Smooth Muscle of Rats and Their Mechanisms
136 Zhang HQ et al. Chinese Herbal Medicines, 212, 4(2): 136-141 Effects of Total Alkaloids in Buxus microphylla Leaves on Aorta Smooth Muscle of Rats and Their Mechanisms ZHANG Hui-qin 1, LIU Yan-yan
More informationSulfur dioxide relaxes rat aorta by endothelium-dependent and. -independent mechanisms
Sulfur dioxide relaxes rat aorta by endothelium-dependent and -independent mechanisms Yang-Kai WANG 1 #, An-Jing REN 1 #, Xiang-Qun YANG 1, Li-Gang WANG 1, Wei-Fang RONG 3, Chao-Shu TANG 4, Wen-Jun YUAN
More informationRelaxant Effects of Matrine on Aortic Smooth Muscles of Guinea Pigs 1
BIOMEDICAL AND ENVIRONMENTAL SCIENCES 22, 327-332 (2009) www.besjournal.com Relaxant Effects of Matrine on Aortic Smooth Muscles of Guinea Pigs 1 JIE ZHENG #, PING ZHENG #, XU ZHOU *, LIN YAN #, RU ZHOU
More informationPeptides-Derived from Thai Rice Bran Improve Hemodynamics and Induce Vasorelaxation in Renovascular Hypertensive Rats
Peptides-Derived from Thai Rice Bran Improve Hemodynamics and Induce Vasorelaxation in Renovascular Hypertensive Rats Orachorn Boonla 1, Phattharaphon Tuangpolkrung 1, Poungrat Pakdeechote 1, Upa Kukongviriyapan
More informationThis laboratory exercise uses a simple preparation and a straightforward
LABORATORY DEMONSTRATION OF VASCULAR SMOOTH MUSCLE FUNCTION USING RAT AORTIC RING SEGMENTS Rayna J. Gonzales, Rebecca W. Carter, and Nancy L. Kanagy Vascular Physiology Group, Department of Cell Biology
More informationThe Antihypertensive Effect of Sesame Seed-derived Products
Bulletin of saka University of Pharmaceutical Sciences 1 (2007) Brief Reviews *,a b c The Antihypertensive Effect of Sesame Seed-derived Products Daisuke NAKAN, *, a Yoshinobu KIS, b Yasuo MATSUMURA c
More informationDifferent Effects of Verapamil on Cytosolic Ca 2+ and Contraction in Norepinephrine-Stimulated Vascular Smooth Muscle
Japan. J. Pharmacol. 55, 35-42 (1991) Different Effects of Verapamil on Cytosolic Ca 2+ and Contraction in Norepinephrine-Stimulated Vascular Smooth Muscle Hideaki Karaki, Koichi Sato and Hiroshi Ozaki
More informationHistamine Develops Homologous Desensitization under Ca 2+ -free Conditions with Increase in Basal Tone in Smooth Muscle of Guinea Pig Taenia Caeci
YAKUGAKU ZASSHI 130(3) 451 455 (2010) 2010 The Pharmaceutical Society of Japan 451 Notes Histamine Develops Homologous Desensitization under Ca 2+ -free Conditions with Increase in Basal Tone in Smooth
More informationThe effect of L-arginine on guinea-pig and rabbit airway smooth muscle function in vitro
Brazilian Journal of Medical and Biological Research (1998) 31: 811-818 L-arginine on airway smooth muscle ISSN -879X 811 The effect of L-arginine on guinea-pig and rabbit airway smooth muscle function
More informationReversal by L-arginine of a dysfunctional arginine/nitric oxide pathway in the endothelium of the genetic diabetic BB rat
Diabetologia (1997) : 91 915 Springer-Verlag 1997 Reversal by L-arginine of a dysfunctional arginine/nitric oxide pathway in the endothelium of the genetic diabetic BB rat G.M. Pieper, W. Siebeneich, G.
More informationPharmacology - Problem Drill 11: Vasoactive Agents
Pharmacology - Problem Drill 11: Vasoactive Agents Question No. 1 of 10 1. Vascular smooth muscle contraction is triggered by a rise in. Question #01 (A) Luminal calcium (B) Extracellular calcium (C) Intracellular
More informationStudies on the effects of viprostol in isolated small blood vessels and thoracic aorta of the rat
Br. J. Pharmacol. (1988), 93, 613-617 Studies on the effects of viprostol in isolated small blood vessels and thoracic aorta of the rat Fong M. Lai, Tarak Tanikella, Agnes Cobuzzi & Peter Cervoni Cardiovascular
More informationReduced capacitative calcium entry in the mesenteric vascular bed of bile duct-ligated rats
European Journal of Pharmacology 525 (2005) 117 122 www.elsevier.com/locate/ejphar Reduced capacitative calcium entry in the mesenteric vascular bed of bile duct-ligated rats Noemí M. Atucha, F. Javier
More informationEvidence for a Role of Cyclic AMP and Endothelium in Rat Aortic Relaxation
6 The Open Circulation and Vascular Journal, 2011, 4, 6-11 Open Access Evidence for a Role of Cyclic AMP and Endothelium in Rat Aortic Relaxation Induced by R-PIA Gonzalo Allende* and Salvador Acevedo
More informationJournal of Chemical and Pharmaceutical Research, 2014, 6(3): Research Article. Partial agonistic property of new isolated natural compounds
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2014, 6(3):1294-1298 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Partial agonistic property of new isolated natural
More informationPrenatal hypoxia causes long-term alterations in vascular endothelin-1 function in aged male but not female offspring
1 2 3 4 5 6 7 8 9 1 11 12 13 14 Supplementary information for: Prenatal hypoxia causes long-term alterations in vascular endothelin-1 function in aged male but not female offspring Stephane L Bourque,
More informationReceptors Families. Assistant Prof. Dr. Najlaa Saadi PhD Pharmacology Faculty of Pharmacy University of Philadelphia
Receptors Families Assistant Prof. Dr. Najlaa Saadi PhD Pharmacology Faculty of Pharmacy University of Philadelphia Receptor Families 1. Ligand-gated ion channels 2. G protein coupled receptors 3. Enzyme-linked
More informationPCTH 400. Endothelial dysfunction and cardiovascular diseases. Blood vessel LAST LECTURE. Endothelium. High blood pressure
PCTH 400 LAST LECTURE Endothelial dysfunction and cardiovascular diseases. Classic Vascular pharmacology -chronic -systemic Local Vascular pharmacology -acute -targeted High blood pressure Blood pressure
More informationReactivity of the isolated perfused rat tail vascular bed
Brazilian Journal of Medical and Biological Research (1997) 30: 891-895 Perfused rat tail vascular bed ISSN 0100-879X 891 Reactivity of the isolated perfused rat tail vascular bed A.S. França, L.V. Rossoni,
More informationThe dynamic regulation of blood vessel caliber
INVITED BASIC SCIENCE REVIEW The dynamic regulation of blood vessel caliber Colleen M. Brophy, MD, Augusta, Ga BACKGROUND The flow of blood to organs is regulated by changes in the diameter of the blood
More informationSevoflurane Inhibits Angiotensin II induced, Protein Kinase C mediated but Not Ca 2 -elicited Contraction of Rat Aortic Smooth Muscle
Anesthesiology 2004; 100:879 84 2004 American Society of Anesthesiologists, Inc. Lippincott Williams & Wilkins, Inc. Sevoflurane Inhibits Angiotensin II induced, Protein Kinase C mediated but Not Ca 2
More informationhypoxic pulmonary hypertension
Br. J. Pharmacol. (1992), 17, 47-413 '." Macmillan Press Ltd, 1992 Reduced relaxant potency of nitroprusside on pulmonary artery preparations taken from rats during the development of hypoxic pulmonary
More informationVascular reactivity in sepsis and platelet dysfunction in septic shock
Vascular reactivity in sepsis and platelet dysfunction in septic shock Benjamin Reddi Discipline of Physiology School of Medical Science University of Adelaide Thesis submitted for the degree of Doctor
More informationModulation of vascular reactivity in normal, hypertensive and diabetic rat aortae by a non-antioxidant flavonoid
Pharmacological Research 55 (2007) 385 391 Modulation of vascular reactivity in normal, hypertensive and diabetic rat aortae by a non-antioxidant flavonoid Machha Ajay a,,1, Francis I. Achike b, Mohd Rais
More informationPKC, Ca 2+, and Myogenic Constriction
PKC, Ca 2+, and Myogenic Constriction Matt Childrey Journal Review of: Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries by: D.H. Korzick,
More informationEffects and mechanisms of Fenofibrate on the secretion of vascular endothelial contraction factors in hypertensive rats
Effects and mechanisms of Fenofibrate on the secretion of vascular endothelial contraction factors in hypertensive rats Y. Zhu 1, H.-S. Wang 1, X.-M. Li 1 and C. Qu 2 1 Department of Cardiac Surgery, General
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationA. HOLiiCYOVA, J. TOROK, I. BERNATOVA, O. PECHANOVA
Physiol. Res. 45: 317-321, 1996 Restriction of Nitric Oxide Rather than Elevated Blood Pressure is Responsible for Alterations of Vascular Responses in Nitric Oxide-Deficient Hypertension A. HOLiiCYOVA,
More informationEffect of ageing on ƒ 1A-adrenoceptor mechanisms in rabbit. Issei TAKAYANAGI, Mann MORIYA and Katsuo KOIKE
J. Smooth Muscle Res. 28: 63-68, 1992. Effect of ageing on ƒ 1A-adrenoceptor mechanisms in rabbit isolated bronchial preparations Issei TAKAYANAGI, Mann MORIYA and Katsuo KOIKE Department of Chemical Pharmacology,
More informationWestern medical The herb is indicated in the treatment of pneumonia, mouth ulcer, hoarse voice, esophagitis, etc.
Bei Sha Shen Introduction Radix glehniae is the dried root of Glehnia littoralis Fr. Schmidt ex Miq. (Fam. Umbelliferae). The drug is collected in summer and autumn, removed from rootlet, washed clean,
More informationCooling effects on nitric oxide production by rabbit ear and femoral arteries during cholinergic stimulation
Br. J. Pharmacol. (1994), 113, 55-554 '." Macmillan Press Ltd, 1994 Cooling effects on nitric oxide production by rabbit ear and femoral arteries during cholinergic stimulation N. Fernandez, L. Monge,
More informationAstragaloside IV Improves Metabolic Syndrome and Endothelium Dysfunction in Fructose-Fed Rats
Molecules 2011, 16, 3896-3907; doi:10.3390/molecules16053896 Article OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules Astragaloside IV Improves Metabolic Syndrome and Endothelium Dysfunction
More informationDILATATION RESERVE OF PULMONARY ARTERIES AT STAGES OF THE CHRONIC OBSTRUCTIVE PULMONARY DISEASE MODEL
Manuscript type: Original Article DOI: 10.5152/TurkThoracJ.2019.18197 Title: DILATATION RESERVE OF PULMONARY ARTERIES AT STAGES OF THE CHRONIC OBSTRUCTIVE PULMONARY DISEASE MODEL Short Title: Pulmonary
More informationTHE BRONCHORELAXANT EFFECT OF HELICIDINE, A HELIX POMATIA EXTRACT, INVOLVES PROSTAGLANDIN E 2 RELEASE
Pharmaceutical Biology 1388-0209/98/3601-0013$12.00 1998, Vol. 36, No. 1, pp. 13 19 Swets & Zeitlinger THE BRONCHORELAXANT EFFECT OF HELICIDINE, A HELIX POMATIA EXTRACT, INVOLVES PROSTAGLANDIN E 2 RELEASE
More informationRelaxation mechanisms of neferine on the rabbit corpus cavernosum tissue in vitro
Asian J Androl 2007; 9 (6): 795 800 DOI: 10.1111/j.1745-7262.2007.00321.x www.asiaandro.com. Original Article. Relaxation mechanisms of neferine on the rabbit corpus cavernosum tissue in vitro Jun Chen
More informationMg 2 -induced endothelium-dependent relaxation of blood vessels and blood pressure lowering: role of NO
Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278: R628 R639, 2000. Mg 2 -induced endothelium-dependent relaxation of blood vessels and blood pressure lowering: role of NO ZHI-WEI YANG, 1 ASEFA
More informationLiu Jing and Liu Jing Diagnosis System in Classical TCM Discussions of Six Divisions or Six Confirmations Diagnosis System in Classical TCM Texts
Liu Jing and Liu Jing Diagnosis System in Classical TCM Discussions of Six Divisions or Six Confirmations Diagnosis System in Classical TCM Texts Liu Jing Bian Zheng system had developed about 1800 years
More informationCanqiu Yu 1, Jinwei Chen 2, Li Huang 3*
A STUDY ON THE ANTITUMOUR EFFECT OF TOTAL FLAVONOIDS FROM PTERIS MULTIFIDA POIR IN H22 TUMOUR-BEARING MICE 459 Canqiu Yu 1, Jinwei Chen 2, Li Huang 3* 1 Department of General Surgery, The Second Xiangya
More informationYin Xia 1,2 and Raouf A. Khalil 1 1
Am J Physiol Heart Circ Physiol 31: H1851 H1865, 16. First published May 3, 16; doi:1.1152/ajpheart.876.15. nancy-associated adaptations in [Ca 2 ] i -dependent and Ca 2 sensitization mechanisms of venous
More informationCellular Messengers. Intracellular Communication
Cellular Messengers Intracellular Communication Most common cellular communication is done through extracellular chemical messengers: Ligands Specific in function 1. Paracrines Local messengers (neighboring
More informationHeart Failure (HF) Treatment
Heart Failure (HF) Treatment Heart Failure (HF) Complex, progressive disorder. The heart is unable to pump sufficient blood to meet the needs of the body. Its cardinal symptoms are dyspnea, fatigue, and
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(3):214-218 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Extraction Optimization and Antioxidant Activity
More informationGUEI-JANE WANG, ANDREW YAU-CHIK SHUM, 1 YUN-LIAN LIN, JYH-FEI LIAO, 1 XI-CHEN WU, 2 JUN REN, 3 and CHIEH-FU CHEN
0022-3565/01/2971-240 246$3.00 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Vol. 297, No. 1 Copyright 2001 by The American Society for Pharmacology and Experimental Therapeutics 3523/893779
More informationTHE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY
Page: 1 of 5 1. Risk Assessment: This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this procedure. Therefore,
More informationSupplemental Figure I
Supplemental Figure I Kl ( mmol/l)-induced Force orta M (mn) 1 (mn) 1 Supplemental Figure I. Kl-induced contractions. and, Kl ( mmol/l)-induced contractions of the aorta () and those of mesenteric arteries
More informationMultiple factors contributing to lipopolysaccharide-induced reactivity changes in rabbit pulmonary artery
Acta Physiologica Sinica, December 25, 2005, 57 (6): 737-741 http://www.actaps.com.cn 737 Research Paper Multiple factors contributing to lipopolysaccharide-induced reactivity changes in rabbit pulmonary
More informationAmniotic fluid stem cells provide considerable advantages in epidermal. regeneration: B7H4 creates a moderate inflammation
Amniotic fluid stem cells provide considerable advantages in epidermal regeneration: B7H4 creates a moderate inflammation microenvironment to promote wound repair Qing Sun 1, +, Fang Li 1, +, Hong Li 2,
More informationDoes Phospholipase C Mediate Muscarinic Receptor-Induced Rat Urinary Bladder Contraction?
0022-3565/07/3223-998 1002$20.00 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Vol. 322, No. 3 Copyright 2007 by The American Society for Pharmacology and Experimental Therapeutics 125393/3250270
More informationInhibition of ATP-sensitive potassium channels attenuates propofol-induced vasorelaxation
Inhibition of ATP-sensitive potassium channels attenuates propofol-induced vasorelaxation Chen-Fuh Lam, Pei-Jung Chang, Yung-An Chen, Chin-Yi Yeh and Yu-Chuan Tsai Propofol (2,6-diisopropylphenol) is an
More informationThe role of angiotensin II (AngII) in maintaining
AJH 1999;12:705 715 Chronic Captopril Administration Decreases Vasodilator Responses in Skeletal Muscle Arterioles Jefferson C. Frisbee, David S. Weber, and Julian H. Lombard Changes in arteriolar reactivity
More informationResearch on Extraction Process of Gallic Acid from Penthorum chinense Pursh by Aqueous Ethanol
Green and Sustainable Chemistry, 2015, 5, 63-69 Published Online May 2015 in SciRes. http://www.scirp.org/journal/gsc http://dx.doi.org/10.4236/gsc.2015.52009 Research on Extraction Process of Gallic Acid
More informationResearch Article Vasorelaxant Effect of Osterici Radix Ethanol Extract on Rat Aortic Rings
Evidence-Based Complementary and Alternative Medicine Volume 213, Article ID 35964, 8 pages http://dx.doi.org/1.1155/213/35964 Research Article Vasorelaxant Effect of Osterici Radix Ethanol Extract on
More informationAnalysis of the fatty acids from Periploca sepium by GC-MS and GC-FID
Analysis of the fatty acids from Periploca sepium by GC-MS and GC-FID Ling Tong, Lei Zhang, Shuanghui Yu, Xiaohui Chen, Kaishun Bi * Department of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road
More informationSUPPLEMENTAL DATA. Lumen area ( m 2 )
Elastin Lumen area ( m 2 ) Media to lumen ratio (x1) H.E. Medium thickness ( m) Medium area ( m 2 ) SUPPLEMENTAL DATA A (Bmal1 flox/flox ) (SM-Bmal1 -/- ) B 1 8 8 6 6 4 4 2 2 1µm 5 8 4 6 3 2 4 1 2 Supplemental
More informationCells Mediate Relaxation via Nitric Oxide in Rat Aorta
Platelet-derived Growth Factor Receptors on Macrovascular Endothelial Cells Mediate Relaxation via Nitric Oxide in Rat Aorta Leslie D. Cunningham, Peter Brecher, and Richard A. Cohen Vascular Biology Unit,
More informationTamoxifen Acutely Relaxes Coronary Arteries by an Endothelium-, Nitric Oxide-, and Estrogen Receptor- Dependent Mechanism
0022-3565/00/2952-0519$03.00/0 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Vol. 295, No. 2 Copyright 2000 by The American Society for Pharmacology and Experimental Therapeutics 2429/855705
More informationYuan et al., Afr J Tradit Complement Altern Med. (2014) 11(4):
IZALPININ FROM FRUITS OF ALPINIA OXYPHYLLA WITH ANTAGONISTIC ACTIVITY AGAINST THE RAT BLADDER CONTRACTILITY 120 Yuan Yuan 1,5#, Yin-Feng Tan 2,3,4#, Peng Xu 2,3,4, Hailong Li 2,3,4, Yong-Hui Li 2,3,4,
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL Artepillin C, is it a good marker for quality control of Brazilian Green Propolis? Cui-ping Zhang 1, Xiao-ge Shen 1, Jia-wei Chen 1, Xia-sen Jiang 1, Kai Wang 2, Fu-liang Hu 1 *
More informationRole of Ion Fluxes in Hydrogen Peroxide Pulmonary Vasoconstriction
Physiol. Res. 44: 31-3 7, 1995 Role of Ion Fluxes in Hydrogen Peroxide Pulmonary Vasoconstriction J. W ILHELM, J. H E R G E T 1 Department of Medical Chemistry and Biochemistry and department of Physiology,
More informationFundamentals of Traditional Chinese Medicine
Fundamentals of Traditional Chinese Medicine World Century Compendium to TCM Volume 1 Volume 2 Volume 3 Volume 4 Volume 5 Volume 6 Volume 7 Fundamentals of Traditional Chinese Medicine by Hong-zhou Wu,
More informationReDUcInG RIsK of cardiovascular DIsease
ReDUcInG RIsK of cardiovascular DIsease Student author Puja Banerjee is a senior, dual majoring in biological engineering and biochemistry at Purdue University. She has been working in the physiology laboratory
More informationEMPEROR'S COLLEGE MTOM COURSE SYLLABUS HERB FORMULAE II
COURSE DESCRIPTION The second of three courses in the Herb Formulae series. Categories covered in Formulae II include the Tonify Qi and Blood, Regulate Qi, Invigorate the Blood, Stop Bleeding, Stabilize
More informationDrug Receptor Interactions and Pharmacodynamics
Drug Receptor Interactions and Pharmacodynamics Dr. Raz Mohammed MSc Pharmacology School of Pharmacy 22.10.2017 Lec 6 Pharmacodynamics definition Pharmacodynamics describes the actions of a drug on the
More informationResearches on Fermentation Engineering of Polysaccharide of
13 1 Vol13 No1 1 2009 2 Life Science Research Feb 2009 1a 1b, 2, 1 416000 2 416000 3 410300 : (Cordyceps militaris), :, 6% 1% 25, ; 6% 1% 22, : ; ; ; ; ; : TQ92 : A : 1007-7847(2009)01-0065-06 Researches
More informationMagnesium is a key ionic modulator of blood vessel
Hypomagnesemia Inhibits Nitric Oxide Release From Coronary Endothelium: Protective Role of Magnesium Infusion After Cardiac Operations Paul J. Pearson, MD, PhD, Paulo R. B. Evora, MD, PhD, John F. Seccombe,
More informationLactate and force production in skeletal muscle
J Physiol 562.2 (2005) pp 521 526 521 Lactate and force production in skeletal muscle Michael Kristensen, Janni Albertsen, Maria Rentsch and Carsten Juel Copenhagen Muscle Research Centre, University of
More informationOptimization of Enzyme-assisted Ultrasonic Extraction of Total Ginsenosides from Ginseng Roots Guangna LIU, Yulin ZUO, Jing ZHANG
2019 2nd International Conference on Computer Science and Advanced Materials (CSAM 2019) Optimization of Enzyme-assisted Ultrasonic Extraction of Total Ginsenosides from Ginseng Roots Guangna LIU, Yulin
More informationHYPOTHALAMIC OBESE AND NON OBESE RATS EXPRESS A SIMILAR FUNCTIONAL MUSCARINIC M 3 SUBTYPE IN THE CONDUCTANCE ARTERY
International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 9-1491 Vol 3, Issue 2, 211 Research Article HYPOTHALAMIC OBESE AND NON OBESE RATS EXPRESS A SIMILAR FUNCTIONAL MUSCARINIC M 3 SUBTYPE
More informationTHE EFFECT OF ORGANIC AND AQUEOUS EXTRACTS OF SALVIA FRUTICOSA ON INTESTINAL MOTILITY 13
An-Najah J. Res., Vol.11, No. 7, ( 1992 ) Short Communication Al-Khalil, et al. THE EFFECT OF ORGANIC AND AQUEOUS EXTRACTS OF SALVIA FRUTICOSA ON INTESTINAL MOTILITY 13 BY SULEIMAN AL-KHALIL *, SAADEH
More informationFig. S1. High K+ increases intracellular calcium level.
Fig. S1. High K + increases intracellular calcium level. (A) Neuronal activation measured by calcium imaging using Fura-2. Intracellular calcium levels were continuously monitored by the fura-2 florescence
More informationRAPID COMMUNICATION. Vascular Reactivity in Isolated Lungs of Rats with Spontaneous Systemic Hypertension
Physiol. Res. 40:367-371,1991 RAPID COMMUNICATION Vascular Reactivity in Isolated Lungs of Rats with Spontaneous Systemic Hypertension V. HAMPL, J. HERGET Department of Physiology, 2nd Medical School,
More informationNoradrenaline-induced changes in intracellular Ca 2+ and tension in mesenteric arteries from diabetic rats
British Journal of Pharmacology (2001) 134, 179 ± 187 ã 2001 Nature Publishing Group All rights reserved 0007 ± 1188/01 $15.00 Noradrenaline-induced changes in intracellular Ca 2+ and tension in mesenteric
More informationFull-length article. Abstract. Key words. Introduction. Materials and methods
Acta Pharmacol Sin 2007 May; 28 (5): 643 650 Full-length article Hypertonic and isotonic potassium solutions have different effects on vessel contractility resulting in differences in optimal resting tension
More informationSkeletal Muscle Contraction 4/11/2018 Dr. Hiwa Shafiq
Skeletal Muscle Contraction 4/11/2018 Dr. Hiwa Shafiq Skeletal Muscle Fiber About 40 per cent of the body is skeletal muscle, and 10 per cent is smooth and cardiac muscle. Skeletal muscles are composed
More informationWe also tested the hypothesis that the inhibitory effects of etomidate and ketamine involved a decrease in
Anesthesiology 2003; 98:104 13 2003 American Society of Anesthesiologists, Inc. Lippincott Williams & Wilkins, Inc. Inhibitory Effects of Etomidate and Ketamine on Adenosine Triphosphate Sensitive Potassium
More informationConstruction of the Vesselcollateral. Guidance for Prevention and Treatment of Vasculopathy. Section 1. China. Clinical Trials.
Section 1 05 Clinical Trials Construction of the Vesselcollateral Theory and its Guidance for Prevention and Treatment of Vasculopathy China Yiling Wu The Integration of Traditional and Western Medical
More informationCOMPARATIVE ANALGESIC AND ANTI-INFLAMMATORY EFFECT OF ANGELICA SINENSIS PIECES AND ITS PRODUCTS IN MICE
: 1877-1883 ISSN: 2277 4998 COMPARATIVE ANALGESIC AND ANTI-INFLAMMATORY EFFECT OF ANGELICA SINENSIS PIECES AND ITS PRODUCTS IN MICE WENJUAN W 1, DANDAN C 1, YINGYING Z 2, HONGMENG Z 3 AND DEMIN G 1* 1:
More informationLab Period: Name: Physiology Chapter 14 Blood Flow and Blood Pressure, Plus Fun Review Study Guide
Lab Period: Name: Physiology Chapter 14 Blood Flow and Blood Pressure, Plus Fun Review Study Guide Main Idea: The function of the circulatory system is to maintain adequate blood flow to all tissues. Clinical
More informationRole of Endothelial Nitric Oxide in Shear Stress Induced Vasodilation of Human Microvasculature
Role of Endothelial Nitric Oxide in Shear Stress Induced Vasodilation of Human Microvasculature Diminished Activity in Hypertensive and Hypercholesterolemic Patients Oscar A. Paniagua, MD; Melissa B. Bryant,
More informationA Small Synthetic Molecule Forms Selective Potassium Channels to. Regulate Cell Membrane Potential and Blood Vessel Tone
Electronic Supplementary Material (ESI) for rganic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information A Small Synthetic Molecule Forms Selective
More informationThe Effect of Crude Drug Extracts Bathing (III) - The effect of phthalides from Cnidii rhizoma-
The Effect of Crude Drug Extracts Bathing (III) - The effect of phthalides from Cnidii rhizoma- Hidenori YOROZU, Hirotaka SATO Tochigi Research Laboratories, KAO Corporation Yoshiaki KOMOTO Department
More informationAn excessive increase in glutamate contributes to glucose-toxicity in. β-cells via activation of pancreatic NMDA receptors in rodent diabetes
An excessive increase in glutamate contributes to glucose-toxicity in β-cells via activation of pancreatic NMDA receptors in rodent diabetes Xiao-Ting Huang 1, Chen Li 1,3, Xiang-Ping Peng 1, Jia Guo 1,5,
More informationhttp://noodlemaz.wordpress.com/category/science/cancer/ Outline Introduction Serious nature of Cardiovascular Disease (CVD) How to prevent CVD? The disease process Damage and plaque development Current
More informationThe Journal of Veterinary Medical Science
Advance Publication The Journal of Veterinary Medical Science Accepted Date: Nov 0 J-STAGE Advance Published Date: Nov 0 FULL PAPER Pharmacology Eukaryotic elongation factor kinase inhibitor, A inhibits
More informationFormononetin: A Phytoestrogen and Isoflavone, Relaxes Guinea Pig Gallbladder Strips
Original Article Cell Mol Med Res. 2017;000(000):000-000 Formononetin: A Phytoestrogen and Isoflavone, Relaxes Guinea Pig Gallbladder Strips Loren W. Kline a, c, Edward Karpinski b Abstract Background:
More informationgravid rat myometrial activity
Research Paper Effect of functional modulation of Ca 2+ -activated Cl - currents on gravid rat myometrial activity P. G. Adaikan, A. Adebiyi ABSTRACT Departments of Obstetrics and Gynaecology, National
More informationRole of GABA B Receptor and L-Arg in GABA- Induced Vasorelaxation in Non-diabetic and Streptozotocin- Induced Diabetic Rat Vessels
Iranian Biomedical Journal 19 (2): 91-95 (April 2015) DOI: 10.6091/ibj.1461.2015 Role of GABA B Receptor and L-Arg in GABA- Induced Vasorelaxation in Non-diabetic and Streptozotocin- Induced Diabetic Rat
More informationWarm Up! Test review (already! ;))
Warm Up! Test review (already! ;)) Write a question you might find on the Unit 5 test next week! (Multiple choice, matching, fill in, or short answer!) - challenge yourself and be ready to share!!! PowerPoint
More informationVascular action of the hypoglycaemic agent gliclazide in diabetic rabbits
Diabetologia (1998) 41: 9--15 Springer-Verlag 1998 Vascular action of the hypoglycaemic agent gliclazide in diabetic rabbits P. J. Pagano 1, M. C. Griswold 1, D. Ravel 2, R. A. Cohen 1 1 Vascular Biology
More informationSkeletal Muscle Contraction 5/11/2017 Dr. Hiwa Shafiq
Skeletal Muscle Contraction 5/11/2017 Dr. Hiwa Shafiq Skeletal Muscle Fiber About 40 per cent of the body is skeletal muscle, and 10 per cent is smooth and cardiac muscle. Skeletal muscles are composed
More information1. Antihypertensive agents 2. Vasodilators & treatment of angina 3. Drugs used in heart failure 4. Drugs used in arrhythmias
1. Antihypertensive agents 2. Vasodilators & treatment of angina 3. Drugs used in heart failure 4. Drugs used in arrhythmias Only need to know drugs discussed in class At the end of this section you should
More informationProf. Isabelle Six INSERM Unit 1088 Jules Verne University of Picardie Amiens, France. Slide 1. Slide 2
Effects of phosphate on vascular function New insights Isabelle Six, Amiens, France Chairs: Griet Glorieux, Ghent, Belgium Alberto Ortiz, Madrid, Spain Prof. Isabelle Six INSERM Unit 1088 Jules Verne University
More informationPregnanolone effects on the blood pressure of stress-induced hypertension in rats
Acta Physiologica Sinica August June 25 25 2004 2004 56 56 (3) (4) 269-274 471-475 http//www.actaps.com.cn 471 * 130021 (pregnanolone ) (stress-induced hypertension SIH) (0.24 mg/kg) (angiotensin Ang )
More informationAsymmetric dimethylarginine reduced erythrocyte deformability in streptozotocin-induced diabetic rats
Microvascular Research 73 (2007) 131 136 www.elsevier.com/locate/ymvre Asymmetric dimethylarginine reduced erythrocyte deformability in streptozotocin-induced diabetic rats Zhi-Chun Yang a, Ke Xia b, Li
More informationHypotensive Effect of Agmatine, Arginine Metabolite, is Affected by NO Synthase
Physiol. Res. 53: 357-363, 2004 Hypotensive Effect of Agmatine, Arginine Metabolite, is Affected by NO Synthase M. GEROVÁ, J. TÖRÖK Institute of Normal and Pathological Physiology, Slovak Academy of Sciences,
More information