a-dS. Short title: Create 1 new species (Ampivirus A) in a new genus (Ampivirus) (e.g. 6 new species in the genus Zetavirus) Modules attached

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1 This form should be used for all taxonomic proposals. Please complete all those modules that are applicable (and then delete the unwanted sections). For guidance, see the notes written in blue and the separate document Help with completing a taxonomic proposal Please try to keep related proposals within a single document; you can copy the modules to create more than one genus within a new family, for example. MODULE : TITLE, AUTHORS, etc Code assigned: a-dS (to be completed by ICTV officers) Short title: Create new species (Ampivirus A) in a new genus (Ampivirus) (e.g. 6 new species in the genus Zetavirus) Modules attached (modules and are required) Author(s): Roland Zell, Eric Delwart, Alexander E. Gorbalenya, Tapani Hovi, Andrew M.Q. King, Nick J. Knowles, A. Michael Lindberg, Mark A. Pallansch, Ann C. Palmenberg, Gabor Reuter, Peter Simmonds, Tim Skern, Glyn Stanway and Teruo Yamashita Corresponding author with address: Roland Zell (roland.zell@med.uni-jena.de) List the ICTV study group(s) that have seen this proposal: A list of study groups and contacts is provided at If in doubt, contact the appropriate subcommittee chair (fungal, invertebrate, plant, prokaryote or vertebrate viruses) Picornaviridae Study Group ICTV Study Group comments (if any) and response of the proposer: Date first submitted to ICTV: 5/06/206 Date of this revision (if different to above): ICTV-EC comments and response of the proposer: Page of 0

2 MODULE 2: NEW SPECIES creating and naming one or more new species. If more than one, they should be a group of related species belonging to the same genus. All new species must be placed in a higher taxon. This is usually a genus although it is also permissible for species to be unassigned within a subfamily or family. Wherever possible, provide sequence accession number(s) for one isolate of each new species proposed. Code aS (assigned by ICTV officers) To create new species within: Genus: Ampivirus (new) Subfamily: Family: Picornaviridae Order: Picornavirales Name of new species: Representative isolate: (only per species please) Fill in all that apply. If the higher taxon has yet to be created (in a later module, below) write (new) after its proposed name. If no genus is specified, enter unassigned in the genus box. Ampivirus A newt picornavirus [newt/203/hun] KP77040 GenBank sequence accession number(s) Reasons to justify the creation and assignment of the new species: Explain how the proposed species differ(s) from all existing species. o If species demarcation criteria (see module 3) have previously been defined for the genus, explain how the new species meet these criteria. o If criteria for demarcating species need to be defined (because there will now be more than one species in the genus), please state the proposed criteria. Further material in support of this proposal may be presented in the Appendix, Module A novel picornavirus was detected in faecal samples of the smooth newt (Lissotriton vulgaris) in Hungary. Virus isolates are not available. The sequence exhibits significant similarity to picornaviruses and a picornavirus genome layout (compare Figure ): VPg+5'UTR[AB-C-D/2A-2B-2C Hel /3A-3B VPg -3C Prot -3D Pol ]3'UTR-poly(A) Ampivirus proteins P, 2C and 3CD show only very low amino acid similarities with the orthologous proteins of other picornaviruses. Alignments reveal amino acid identities ranging from: P:.-6.0%, 2C Hel : %, 3D Pol : %. Further typical picornavirus features of the ampivirus polyprotein sequence are: (i) two rhv-like domains (Pfam database) corresponding to VP0, VP3 (ii) NTP-binding motif of 2C Hel (G447DSQCGKT, Walker A motif, and a D499DLLQ motif) (iii) putative 3C proteinase catalytic triad (H96, D2025, D2089ICG) (iv) RNA-dependent RNA polymerase motifs (K235DELR, G2494LPSG, L2539GDD, F2598LKS) Phylogenetic trees comprising reference sequences of the order Picornavirales indicate that ampivirus clusters at the root of the picornavirus branch (compare Figure 2; Appendix). Figures 3, 4 (Appendix) show phylogenetic trees of the picornavirus P and 3CD proteins. Page 2 of 0

3 MODULE 3: NEW GENUS creating a new genus Ideally, a genus should be placed within a higher taxon. Code bS (assigned by ICTV officers) To create a new genus within: Subfamily: Family: Picornaviridae Order: Picornavirales Fill in all that apply. If the higher taxon has yet to be created (in a later module, below) write (new) after its proposed name. If no family is specified, enter unassigned in the family box naming a new genus Code cS (assigned by ICTV officers) To name the new genus: Ampivirus Assigning the type species and other species to a new genus Code dS (assigned by ICTV officers) To designate the following as the type species of the new genus Ampivirus A Every genus must have a type species. This should be a well characterized species although not necessarily the first to be discovered The new genus will also contain any other new species created and assigned to it (Module 2) and any that are being moved from elsewhere (Module 7b). Please enter here the TOTAL number of species (including the type species) that the genus will contain: Reasons to justify the creation of a new genus: Additional material in support of this proposal may be presented in the Appendix, Module Ampivirus is the most divergent picornavirus presently known (Figures 2, 4; Appendix). It has only little sequence similarity to the other picornaviruses. It is the first picornavirus with a capsid protein (VP) that has similarity to the CRPV capsid superfamily (Pfam database) of the dicistroviruses, iflaviruses, marnaviruses and some diatom viruses (labyrnavirus, bacillarnavirus) (Figure ; Appendix). Other unique features of Ampivirus are rather large 3C Pro and 3D Pol proteins. Origin of the new genus name: Ampivirus: from amphibian picornavirus Reasons to justify the choice of type species: only a single species Page 3 of 0

4 Species demarcation criteria in the new genus: If there will be more than one species in the new genus, list the criteria being used for species demarcation and explain how the proposed members meet these criteria. only a single species Page 4 of 0

5 MODULE : APPENDIX: supporting material additional material in support of this proposal References: Reuter G, Boros A, Toth Z, Phan TG, Delwart E, Pankovics P A highly divergent picornavirus in an amphibian, the smooth newt (Lissotriton vulgaris). J Gen Virol 96: Annex: Include as much information as necessary to support the proposal, including diagrams comparing the old and new taxonomic orders. The use of Figures and Tables is strongly recommended but direct pasting of content from publications will require permission from the copyright holder together with appropriate acknowledgement as this proposal will be placed on a public web site. For phylogenetic analysis, try to provide a tree where branch length is related to genetic distance. Genome organization: Fig. Ampivirus A (KP77040) 5'-NTR 970 nt nt 97 M AB rhv 337 aa Q 884 A 885 Q 337 G 338 E 606 S 607?? Q 298 T 299 Q 674 F 675 Q 920 T 92 Q 25 M 252 C rhv 269 aa D CRPV 2A 2B 2C Hel 3A 377 aa 20 aa 3B 36 aa 3C Pro 23 aa 3D Pol 562 aa nt 92 N 273 3'-NTR 34nt Figure : Schematic depiction of the Ampivirus genome organization. The open reading frames are indicated by boxes. Positions of putative aa cleavage sites and the lengths of the deduced proteins are shown as proposed by Reuter et al. (205). Arrows ( ) indicate the putative 3C pro processing sites, whereas question marks (?) hint at two unknown cleavage sites. The sizes of the respective proteins (shaded gray) is unclear. Rhv and CRPV domains proposed by the Pfam database are also indicated Page 5 of 0

6 00 AB050782, Fabavirus, Patchouli mild mosaic virus 99 AB084450, Fabavirus, Broad bean wilt virus 00 NC_003549, Comovirus, Cowpea mosaic virus 59 D0095, Nepovirus, Grapevine fanleaf virus 93 NC_003785, Sadwavirus, Satsuma dwarf virus DQ388879, Torradovirus, Tomato torrado virus AJ62357, Cheravirus, Cherry rasp leaf virus Secoviridae 00 NC_003628, Sequivirus, Parsnip yellow fleck virus EU980442, Sequivirus, Carrot necrotic dieback virus [Anthriscus] 76 NC_00632, Waikavirus, Rice tungro spherical virus 00 U67839, Waikavirus, Maize chlorotic dwarf waikavirus [Tennessee] AY365064, Ectropis obliqua picorna-like virus Iflaviridae AB000906, Iflavirus, Infectious flacherie virus AF092924, Sacbrood virus Iflaviridae 58 NC_004830, Deformed wing virus Iflaviridae Iflaviridae 99 NC_0437, Slow bee paralysis virus (Iflaviridae) KF360262, unassigned, Aphis glycines virus [ApGlV] 00 KM05260, unassigned, Soybean-associated bicistronic virus [North Dakota] 00 KJ420969, unassigned, Arivirus [A09777/676/200] KJ420970, unassigned, Arivirus 2 [A09777/676/200] KM07736, unassigned, Fesavirus KM259869, unassigned, Antarctic picorna-like virus 00 KJ802403, unassigned, Cripavirus [NB-/20/HUN] AB07037, Cripavirus, Himetobi P virus AF022937, Cripavirus, Rhopalosiphum padi virus EU282007, Cripavirus, Rhopalosiphum padi virus [RhPV6] NC_003924, Cripavirus, Cricket paralysis virus NC_00834, Cripavirus, Drosophila C virus 64 NC_006559, Aparavirus, Solenopsis invicta virus- 00 AF50629, Aparavirus, Acute bee paralysis virus NC_00528, Marnavirus, Heterosigma akashiwo RNA virus [SOG263] AB93726, Labyrnavirus, Aurantiochytrium ssrna virus KM25987, unassigned, Antarctic picorna-like virus 3 JN6660, unassigned, Marine RNA virus SF KF478836, unassigned, Marine RNA virus SF-3 KF4290, unassigned, Marine RNA virus SF-2 EF98242, unassigned, Marine RNA virus JP-B KM259872, unassigned, Antarctic picorna-like virus 4 NC_024489, unassigned, Asterionellopsis glacialis RNA virus NC_0222, Bacillarnavirus, Chaetoceros socialis f. radians RNA virus NC_0863, Bacillarnavirus, Rhizosolenia setigera RNA virus-0 AB375474, Bacillarnavirus, Chaetoceros tenuissimus RNA virus KM259870, unassigned, Antarctic picorna-like virus 2 NC_025889, unassigned, Chaetoceros tenuissimus RNA virus type-ii [SS0-6V] NC_009757, unassigned, Marine RNA virus JP-A AB639040, unassigned, Chaetoceros sp. RNA virus 2 [Csp02RNAV0] KT25902, unassigned, Husavirus [ACS78] NC_023637, unassigned, Posavirus NC_023638, unassigned, Posavirus 2 KU75459, unassigned, Pow Burn virus 00 KM434233, unassigned, Fisavirus [HAL] NC_0648, unassigned, Halastavi arva RNA virus KP77040, Ampivirus, Ampivirus A [NEWT/203/HUN] 00 AF40683, Sapelovirus, Sapelovirus A [PEV8-V3] 60 V049, Enterovirus, Enterovirus C poliovirus [Mahoney] M886, Cardiovirus, Cardiovirus A [R] 76 KC935379, Kunsagivirus, Kunsagivirus A 56 JQ36470, Pasivirus, Pasivirus A [swine/france/20] 98 L0297, Parechovirus, Parechovirus A [Harris] 70 AB937989, unassigned, Crohivirus [shrew/zm54/zambia/202] 0,5 Dicistroviridae Marnaviridae Picornaviridae Figure 2: Phylogenetic analyses of the 3CD protein of representative members of the Picornavirales using maximum likelihood tree inference (MEGA 5.2). 60 sequences were retrieved from GenBank. Presented are GenBank accession numbers, genus names, species names and types. If available, designations of isolates [in square brackets] are given. Yet unassigned viruses are printed in blue. Proposed Ampivirus is printed in red and indicated by a dot ( ). Numbers at nodes indicate bootstrap values obtained after 200 replications. The optimal substitution model (GTR+G+I) was determined with MEGA 5.2. The scale indicates substitutions/site. Page 6 of 0

7 P HQ595340, unassigned, bat picornavirus [NC6A] HQ59534, unassigned, bat picornavirus [LMH22A] HQ595342, unassigned, bat picornavirus 2 [MH9F] HQ595343, unassigned, bat picornavirus 2 [SK7F] 0.84 JN83356, unassigned, canine picornavirus [dog/hong Kong/325F/2008] KJ64694, unassigned, bat picornavirus [BtRs-PicoV/YN200] JQ84852, unassigned, Ia io picornavirus HQ595344, unassigned, bat picornavirus 3 [TLC5F] HQ595345, unassigned, bat picornavirus 3 [TLC2F] KJ64693, unassigned, bat picornavirus [BtRh-PicoV/SC203] 0.88 JN5727, unassigned, feline picornavirus [356F] JN5729, unassigned, feline picornavirus [02F] JN5725, unassigned, feline picornavirus [cat/hong Kong/073F/2007] JN5726, unassigned, feline picornavirus [27F] 0.87 JN5728, unassigned, feline picornavirus [66F] 0.54 KJ64687, unassigned, bat picornavirus [BtMf-PicoV/FJ202] KJ64690, unassigned, bat picornavirus [BtMf-PicoV-/GD202] KJ64699, unassigned, bat picornavirus [BtMf-PicoV-2/SAX20] 0.85 LC03658, unassigned, bovine picornavirus [Bo-2-/2009/JPN] LC036582, unassigned, bovine picornavirus [Bo-2-38/2009/JPN] LC036579, unassigned, bovine picornavirus [Bo-2-3/2009/JPN] LC036580, unassigned, bovine picornavirus [Bo-2-7/2009/JPN] LC00697, unassigned, bovine picornavirus [Bo--39/2009/JPN] KP345888, unassigned, dromedary camel enterovirus 2 [20CC] KP345887, unassigned, dromedary camel enterovirus [9CC] 0.52 DQ092770, Enterovirus, EV-F BEV-2 [BEV-26-RM2] DQ092769, Enterovirus, EV-E BEV- [LC-R4] AF363453, Enterovirus, EV-G [UKG-40-73] U2252, Enterovirus, EV-A7 [BrCr] AF326766, Enterovirus, EV-J [SV6-63] D00820, Enterovirus, EV-D70 [J670-7] AF20894, Enterovirus, EV-H [A-2 plaque virus] 0.9 K022, Enterovirus, RV-B4 [959] 0.52 EF86077, Enterovirus, RV-C3 [HRV-QPM] L2497, Enterovirus, RV-A6 [757] 0.52 V049, Enterovirus, EV-C PV [Mahoney] M33854, Enterovirus, EV-B CVB3 [Nancy] KP233897, unassigned, rabovirus [Berlin/Jan20/0572] KJ950883, unassigned, rat picornavirus [RPV/NYC-B0] AF40683, Sapelovirus, Sapelovirus A, porcine sapelovirus [V3] AY064708, Sapelovirus, Sapelovirus B, simian sapelovirus [SV2-2383] KJ64696, unassigned, bat picornavirus [BtVs-PicoV/SC203] 0.9 KJ64689, unassigned, bat picornavirus [BtMa-PicoV/FJ202] JN420368, unassigned, Californian sealion sapelovirus [62] JN674502, unassigned, quail picornavirus KT880670, unassigned, phacovirus [Pf-CHK/PhV] FR72744, unassigned, pigeon picornavirus B [pigeon/norway/03/64/2003] KC56080, unassigned, pigeon picornavirus B [GAL-7/200/Hungary] AY563023, Sapelovirus, Avian sapelovirus [TW90A] 0.84 KJ64688, unassigned, bat picornavirus [BtRlep-PicoV/FJ202] KJ64692, unassigned, bat picornavirus [BtRa-PicoV/JS203] KJ64685, unassigned, bat picornavirus [BtRf-PicoV-/YN202] KJ64697, unassigned, bat picornavirus [BtNv-PicoV/SC203] KM87363, unassigned, tortoise picornavirus [9-05] KM87364, unassigned, tortoise picornavirus [44-0] KM87365, unassigned, tortoise picornavirus [203-T4] KM8736, unassigned, tortoise picornavirus [4-04] KM87362, unassigned, tortoise picornavirus [5-04] KM87367, unassigned, tortoise picornavirus [5-03] KM87366, unassigned, tortoise picornavirus [24-4-0] JF973687, Mosavirus, Mosavirus A [M-7] KF95846, Mosavirus, Mosavirus A2 [SZAL6-MoV/20/HUN] KM396707, unassigned, lesavirus [Mis0308/202] KM396708, unassigned, lesavirus 2 [Nai0805/202] JQ94880, Hunnivirus, Hunnivirus A [BHUV/2008/HUN] 0.9 AF23769, Teschovirus, Teschovirus A [Talfan] 0.65 EU236594, Aphthovirus, Bovine rhinitis B virus [EC] JN936206, Aphthovirus, Bovine rhinitis A virus 2 [H-] X0087, Aphthovirus, Foot-and-mouth disease virus O [Kaufbeuren] X96870, Aphthovirus, Equine rhinitis A virus [PERV] X9687, Erbovirus, Equine rhinitis B virus [P436/7] KM589358, unassigned, bovine picornavirus [TCH6] KP054277, unassigned, bat picornavirus [BatPV/V3/3/Hun] KP054276, unassigned, bat picornavirus [BatPV/V2/3/Hun] KP054274, unassigned, bat picornavirus [BatPV/V8/3/Hun] KP054275, unassigned, bat picornavirus [BatPV/V/3/Hun] KP054273, unassigned, bat picornavirus [BatPV/V/3/Hun] KP054278, unassigned, bat picornavirus [BatPV/V4/3/Hun] JQ8485, Mischivirus, Mischivirus A [China/200] KP00644, unassigned, African bat icavirus [TNo3] FJ438902, Cosavirus, Cosavirus A [0553] JN867758, unassigned, human cosavirus F [PK5006] FJ438907, unassigned, human cosavirus B [2263] FJ555055, unassigned, human cosavirus E [Australia/6] FJ438908, unassigned, human cosavirus D [5004] M886, Cardiovirus, Cardiovirus A, encephalomyocarditis virus [R] M20562, Cardiovirus, Cardiovirus B, Theiler's murine encephalomyelitis virus [GDVII] JQ864242, Cardiovirus, Cardiovirus C, boone cardiovirus [rat/usa/200] JX683808, Cardiovirus, Cardiovirus C2, boone cardiovirus 2 [rat/usa/202] DQ64257, Senecavirus, Senecavirus A, Seneca Valley virus [SVV-00] KJ690629, unassigned, chicken proventriculitis virus [CPV/Korea/03] KF9687, unassigned, chicken megrivirus [chicken/chk-iv-chv/203/hun] KF9686, unassigned, chicken megrivirus [chicken/b2-chv/202/hun] KF979336, unassigned, chicken picornavirus 5 [27C] 0.97 HM7599, Megrivirus, Melegrivirus A, turkey hepatitis virus [2993D] KF979335, unassigned, chicken picornavirus 4 [5C] KC663628, unassigned, duck megrivirus [LY] 0.74 KC876003, unassigned, mesivirus [HK2] KC8837, unassigned, mesivirus 2 [pigeon/galii5-pimev/20/hun] JN89202, Dicipivirus, Cadicivirus A, canine picodicistrovirus [209] JF973686, Rosavirus, Rosavirus A, murine rosavirus [M-7] KJ934637, Kobuvirus, Aichivirus A5, roller kobuvirus [SZAL6-KoV/20/HUN] JQ898342, Kobuvirus, Aichivirus A6, Kathmandu sewage kobuvirus [KoV-SewKTM] 0.89 JF755427, Kobuvirus, Aichivirus A3, murine kobuvirus [M-5/USA/200] AB0045, Kobuvirus, Aichivirus A [A846-88] KJ958930, Kobuvirus, Aichivirus A4, feline kobuvirus [2D240] KF83027, Kobuvirus, Aichivirus A4, feline kobuvirus [FK-3] KM09960, Kobuvirus, Aichivirus A4, feline kobuvirus [FeKoV/TE/52/IT/3] JN38733, Kobuvirus, Aichivirus A2, canine kobuvirus [dog-an2d-usa-2009] 0.8 GU245693, Kobuvirus, Aichivirus B3, ovine kobuvirus [sheep/tb3/hun/2009] KF006985, Kobuvirus, Aichivirus B2, ferret kobuvirus [MpKoV38] AB084788, Kobuvirus, Aichivirus B, bovine kobuvirus [U-] EU , Kobuvirus, Aichivirus C, porcine kobuvirus [swine/s-/hun/2007/hungary] KF793927, Kobuvirus, Aichivirus C2, caprine kobuvirus [2Q08] LC055960, unassigned, kagovirus 2 [cattle/kagoshima-2-24-kov/205/jpn] LC05596, unassigned, kagovirus [cattle/kagoshima--22-kov/204/jpn] KJ6469, unassigned, bat picornavirus [BtMf-PicoV-2/GD202] KJ64686, unassigned, bat picornavirus [BtMr-PicoV/JX200] KT325852, unassigned, rabbit picornavirus [Rabbit0/203/HUN] GQ79640, Salivirus, Salivirus A [NG-J] 0.97 KF74227, Sicinivirus, Sicinivirus A [UCC00] 0.82 KF97933, Sicinivirus, Sicinivirus A2, chicken picornavirus [55C] 0.94 JQ6963, Gallivirus, Gallivirus A [turkey/m76/20/hun] GU82406, Passerivirus, Passerivirus A, turdivirus [thrush/hong Kong/00356/2007] 0.6 GU82408, Oscivirus, Oscivirus A, turdivirus 2 [robin/hong Kong/077/2006] 0.72 GU8240, Oscivirus, Oscivirus A2, turdivirus 3 [thrush/hong Kong/0878/2006] KF38772, Sakobuvirus, Sakobuvirus A, feline sakobuvirus [FFUP/Portugal/202] KJ4577, unassigned, tortoise rafivirus [UF4] KF979334, unassigned, chicken picornavirus 3 [45C] 0.97 KT880669, unassigned, chicken picornavirus [Pf-CHK/AsV] KF979333, unassigned, chicken picornavirus 2 [44C] KC465954, Avisivirus, Avisivirus A [turkey/m76-tuasv/20/hun] KC64703, Avisivirus, Avisivirus A [USA-IN] KM203656, unassigned, orivirus [chicken/pf-chk/203/hun] KT880667, unassigned, orivirus 2 [Pf-CHK] KJ000696, unassigned, aalivirus (duck picornavirus) [GL/2] DQ249299, Avihepatovirus, Avihepatovirus A, duck hepatitis A virus [03D] JQ36470, Pasivirus, Pasivirus A, swine pasivirus [swine/france/20] AB937989, unassigned, crohivirus [shew/zm54] 0.57 AF327920, Parechovirus, Parechovirus B, Ljungan virus [87-02] HF677705, unassigned, sebokele virus [An/B/227/d] L0297, Parechovirus, Parechovirus A, human parechovirus [Harris] 0.72 KF006989, unassigned, ferret parechovirus [MpPeV] KJ64698, unassigned, bat picornavirus [BtMf-PicoV-/SAX20] JQ84853, unassigned, Rhinolophus affinis picornavirus KC935379, Kunsagivirus, Kunsagivirus A, roller kunsagivirus [roller/szal6-kuv/20/hun] unassigned, Bat Kunsagivirus [bat/cameroon/203] 0.92 EU42040, Aquamavirus, Aquamavirus A, seal picornavirus [HO-02-2] KC843627, Potamipivirus, Potamipivirus A, eel picornavirus [F5/05] 0.9 KF8395, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm//mn/usa/200] KF8396, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm/2/mn/usa/200] KF874490, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm/20/il/usa/200] KC465953, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [09-283] KF306267, Limnipivirus, Limnipivirus B, carp picornavirus [F37/06] JX34222, Limnipivirus, Limnipivirus A, bluegill picornavirus [04-032] KT452695, unassigned, hedgehog hepatovirus [Igel75Erieur204] KT452698, unassigned, hedgehog hepatovirus [Igel68Erieur204] KT45269, unassigned, hedgehog hepatovirus [Igel8Erieur204] KT45274, unassigned, bat hepatovirus [M32Eidhel200] KT87758, unassigned, tupaia hepatovirus [TN] KT452730, unassigned, bat hepatovirus [BUO2BF86Colafr200] KT452729, unassigned, bat hepatovirus [FOAF48Rhilan200] KT452742, unassigned, bat hepatovirus [SMG8520Minmav204] 0.72 KT452735, unassigned, rodent hepatovirus [CIV459Lopsik2004] KR703607, unassigned, phopivirus [NewEngland/USA/20] KT452637, unassigned, rodent hepatovirus [RMU0637Micarv200] KT45264, unassigned, rodent hepatovirus [KS50Myogla20] KT452644, unassigned, rodent hepatovirus [KS230Crimig20] M4707, Hepatovirus, Hepatovirus A, hepatitis A virus [HM-75] D00924, Hepatovirus, Hepatovirus A, simian hepatitis A virus [AGM-27] KT22962, unassigned, woodchuck hepatovirus [3ID] KT2296, unassigned, woodchuck hepatovirus [2ID] KT452685, unassigned, rodent hepatovirus [KEF2Sigmas202] KT452658, unassigned, shrew hepatovirus [KS2232Sorara202] KT45266, unassigned, shrew hepatovirus [KS2289Sorara202] AJ22573, Tremovirus, Tremovirus A, avian encephalomyelitis virus [Calnek vaccine strain] 0.87 KP77040, Ampivirus, Ampivirus A [NEWT/203/HUN] KP230449, unassigned, falcovirus [kestrel/vove0622/203/hun] 0.5 Page 7 of 0

8 Figure 3 (previous page): Phylogenetic analyses of picornavirus P using Bayesian tree inference (MrBayes 3.2). 78 picornavirus sequences were retrieved from GenBank. Presented are GenBank accession numbers, genus names, species names and types. If available, common names and designations of isolates [in square brackets] are given. Yet unassigned viruses are printed in blue. Proposed name is printed in red and indicated by a dot ( ). Numbers at nodes indicate posterior probabilities obtained after 6,000,000 generations. The optimal substitution model (GTR+G+I) was determined with MEGA 5. The scale indicates substitutions/site. Page 8 of 0

9 3CD KJ958930, Kobuvirus, Aichivirus A4, feline kobuvirus [2D240] KM09960, Kobuvirus, Aichivirus A4, feline kobuvirus [FeKoV/TE/52/IT/3] KF83027, Kobuvirus, Aichivirus A4, feline kobuvirus [FK-3] JN38733, Kobuvirus, Aichivirus A2, canine kobuvirus [dog-an2d-usa-2009] JF755427, Kobuvirus, Aichivirus A3, murine kobuvirus [M-5-USA-200] AB0045, Kobuvirus, Aichivirus A [A846-88] JQ898342, Kobuvirus, Aichivirus A6, Kathmandu sewage kobuvirus [KoV-SewKTM] KJ934637, Kobuvirus, Aichivirus A5, roller kobuvirus [SZAL6-KoV/20/HUN] AB084788, Kobuvirus, Aichivirus B, bovine kobuvirus [U-] GU245693, Kobuvirus, Aichivirus B3, ovine kobuvirus [sheep/tb3/hun/2009] KF006985, Kobuvirus, Aichivirus B2, ferret kobuvirus [ferret/mpkov38/nl/200] EU787450, Kobuvirus, Aichivirus C, porcine kobuvirus [sw/s-/hun/2007] KF793927, Kobuvirus, Aichivirus C2, caprine kobuvirus [2Q08] LC055960, unassigned, kagovirus 2 [cattle/kagoshima-2-24-kov/205/jpn] LC05596, unassigned, kagovirus [cattle/kagoshima--22-kov/204/jpn] GQ79640, Salivirus, Salivirus A [hu/ng-j/nigeria/2007] JQ6963, Gallivirus, Gallivirus A [turkey/hun/m76/20] GU82406, Passerivirus, Passerivirus A, turdivirus [thrush/hong Kong/00356/2007] KF74227, Sicinivirus, Sicinivirus A [chicken/ucc00/eire] KF97933, Sicinivirus, Sicinivirus A [ChPV-/chicken/55C/Hong Kong/2008] KF38772, Sakobuvirus, Sakobuvirus A [FFUP/Portugal/202] KJ64686, unassigned, bat picornavirus [BtMr-PicoV/JX200] KJ6469, unassigned, bat picornavirus [BtMf-PicoV-2/GD202] KT325852, unassigned, rabbit picornavirus [Rabbit0/203/HUN] GU82408, Oscivirus, Oscivirus A, turdivirus 2 [robin/hong Kong/077/2006) GU8240, Oscivirus, Oscivirus A2, turdivirus 3 [robin/hong Kong/ ] KJ4577, unassigned, tortoise rafivirus [UF4] KF9686, unassigned, chicken megrivirus [chicken/b2-chv/202/hun] KF9687, unassigned, chicken megrivirus [chicken/chk-iv-chv/203/hun] KJ690629, unassigned, chicken proventriculitis virus [CPV/Korea/03] 0.5 HM7599, Megrivirus, Melegrivirus A, turkey hepatitis virus [2993D] 0.55 KF979336, unassigned, chicken picornavirus 5 [27C] KF979335, unassigned, chicken picornavirus 4 [5C] KC663628, unassigned, duck megrivirus [LY] KC8837, unassigned, pigeon mesivirus 2 [pigeon/galii5-pimev/20/hun] KC876003, unassigned, mesivirus [HK2] JN89202, Dicipivirus, Cadicivirus A, canine picodicistrovirus [209] JF973686, Rosavirus, Rosavirus A [mouse/usa/m-7/200] KP054278, unassigned, bat picornavirus [BatPV/V4/3/Hun] KP054276, unassigned, bat picornavirus [BatPV/V2/3/Hun] KP054275, unassigned, bat picornavirus [BatPV/V/3/Hun] 0.9 KP054277, unassigned, bat picornavirus [BatPV/V3/3/Hun] KP054274, unassigned, bat picornavirus [BatPV/V8/3/Hun] KP054273, unassigned, bat picornavirus [BatPV/V/3/Hun] JQ8485, Mischivirus, Mischivirus A [bat/china/200] KP00644, unassigned, African bat icavirus [TNo3] M20562, Cardiovirus, Cardiovirus B, Theiler's murine encephalomyelitis virus [GDVII] M886, Cardiovirus, Cardiovirus A, encephalomyocarditis virus [R] JQ864242, Cardiovirus, Cardiovirus C, Boone cardiovirus [rat/usa/200] JX683808, Cardiovirus, Cardiovirus C2, Boone cardiovirus 2 [rat/usa/202] DQ64257, Senecavirus, Senecavirus A, Seneca Valley virus [cc/us/svv-00] FJ438908, unassigned, human cosavirus D [5004] FJ555055, unassigned, human cosavirus E [Australia/6] FJ438907, unassigned, human cosavirus B [2263] FJ438902, Cosavirus, Cosavirus A [0553] JN867758, unassigned, human cosavirus F [PK5006] EU236594, Aphthovirus, Bovine rhinitis B virus [EC] JN936206, Aphthovirus, Bovine rhinitis A virus 2 [H-] X0087, Aphthovirus, Foot-and-mouth disease virus O [Kaufbeuren] X96870, Aphthovirus, Equine rhinitis A virus [PERV] KM396707, unassigned, lesavirus [Mis0308/202] KM396708, unassigned, lesavirus 2 [Nai0805/202] JQ94880, Hunnivirus, Hunnivirus A [cattle/hun/2008] AF23769, Teschovirus, Teschovirus A, porcine teschovirus [Talfan] X9687, Erbovirus, Erbovirus A, equine rhinitis B virus [P436/7] KM589358, unassigned, bovine erbo-like picornavirus [TCH6] KM87363, unassigned, tortoise picornavirus [9-05] JN5729, unassigned, feline picornavirus [02F] JN5725, unassigned, feline picornavirus [cat/hong Kong/073F/20079 JN5726, unassigned, feline picornavirus [27F] JN5728, unassigned, feline picornavirus [66F] KJ64687, unassigned, bat picornavirus [BtMf-PicoV/FJ202] 0.69 KJ64699, unassigned, bat picornavirus [BtMf-PicoV-2/SAX20] KJ64690, unassigned, bat picornavirus [BtMf-PicoV-/GD202] HQ595344, unassigned, bat picornavirus [TLC5F] HQ595345, unassigned, bat picornavirus [TLC2F] KJ64693, unassigned, bat picornavirus [BtRh-PicoV/SC203] HQ595340, unassigned, bat picornavirus [NC6A] HQ59534, unassigned, bat picornavirus [LMH22A] HQ595342, unassigned, bat picornavirus [MH9F] HQ595343, unassigned, bat picornavirus [SK7F] JQ84852, unassigned, Ia io picornavirus KJ64694, unassigned, bat picornavirus [BtRs-PicoV/YN200] JN83356, unassigned, canine picornavirus [dog/hong Kong/325F/2008] LC036579, unassigned, bovine picornavirus [Bo-2-3/2009/JPN] LC03658, unassigned, bovine picornavirus [Bo-2-/2009/JPN] LC036580, unassigned, bovine picornavirus [Bo-2-7/2009/JPN] LC036582, unassigned, bovine picornavirus [Bo-2-38/2009/JPN] LC00697, unassigned, Bovine picornavirus [Bo--39/2009/JPN] AF40683, Sapelovirus, Sapelovirus A, porcine sapelovirus [V3] AY064708, Sapelovirus, Sapelovirus B, simian sapelovirus [SV2-2383] KJ64696, unassigned, bat picornavirus [BtVs-PicoV/SC203] KJ64689, unassigned, bat picornavirus [BtMa-PicoV/FJ202] AY563023, Sapelovirus, Avian sapelovirus [TW90A] JN420368, unassigned, Californian sealion sapelovirus [62] KJ64688, unassigned, bat picornavirus [BtRlep-PicoV/FJ202] KJ64692, unassigned, bat picornavirus [BtRa-PicoV/JS203] KJ64685, unassigned, bat picornavirus [BtRf-PicoV-/YN202] KJ64697, unassigned, bat picornavirus [BtNv-PicoV/SC203] M33854, Enterovirus, EV-B CV-B3 [Nancy] AF326766, Enterovirus, EV-J [SV6-63] D00820, Enterovirus, EV-D70 [J670-7] V049, Enterovirus, EV-C PV [Mahoney] U2252, Enterovirus, EV-A7 [BrCr] KP345888, unassigned, Dromedary camel enterovirus 2 [20CC] KP345887, unassigned, Dromedary camel enterovirus [9CC] DQ092770, Enterovirus, EV-F BEV-2 [BEV-26 RM2] DQ092769, Enterovirus, EV-E BEV- [LC-R4] AF363453, Enterovirus, EV-G [UKG/40/73] AF20894, Enterovirus, EV-H [A-2 plaque virus] L2497, Enterovirus, RV-A6 [757] EF86077, Enterovirus, RV-C3 [HRV-QPM] K022, Enterovirus, RV-B4 [959] KP233897, unassigned, rabovirus [Berlin/Jan20/0572] KJ950883, unassigned, rat picornavirus [rat/nyc-b0/usa/200] FR72744, unassigned, pigeon picornavirus B [pigeon/norway/03/64/2003] KC56080, unassigned, pigeon picornavirus B [GAL-7/200/Hungary] FR72745, unassigned, pigeon picornavirus A [pigeon/norway/03/603-7/2003] JN674502, unassigned, quail picornavirus [quail/hun/200] KT880670, unassigned, phacovirus [Pf-CHK/PhV] KP230449, unassigned, falcovirus [kestrel/vove0622/203/hun] KJ64684, unassigned, bat picornavirus [BtRf-PicoV-2/YN202] KT452637, unassigned, rodent hepatovirus [RMU0637Micarv200] KT45264, unassigned, rodent hepatovirus [KS50Myogla20] KT452644, unassigned, rodent hepatovirus []KS230Crimig20] KT22962, unassigned, woodchuck hepatovirus [3ID] KT2296, unassigned, woodchuck hepatovirus [2ID] KT452685, unassigned, rodent hepatovirus [KEF2Sigmas202] M4707, Hepatovirus, Hepatovirus A, hepatitis A virus [HM-75] D00924, Hepatovirus, Hepatovirus A, simian hepatitis A virus [AGM-27] KT452658, unassigned, shrew hepatovirus [KS2232Sorara202] KT45266, unassigned, shrew hepatovirus [KS2289Sorara202] KT452695, unassigned, hedgehog hepatovirus [Igel75Erieur204] KT452698, unassigned, hedgehog hepatovirus [Igel68Erieur204] KT45269, unassigned, hedgehog hepatovirus [Igel8Erieur204] KT45274, unassigned, bat hepatovirus [M32Eidhel200] KT87758, unassigned, tupaia hepatovirus [TN] KT452730, unassigned, bat hepatovirus [BUO2BF86Colafr200] KT452729, unassigned, bat hepatovirus [FOAF48Rhilan200] KR703607, unassigned, phopivirus [NewEngland/USA/20] KT452742, unassigned, bat hepatovirus [SMG8520Minmav204] KT452735, unassigned, rodent hepatovirus [CIV459Lopsik2004] AJ22573, Tremovirus, Tremovirus A, avian encephalomyelitis virus [Calnek vaccine strain] 0.95KF8395, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm//mn/usa/200] KF8396, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm/2/mn/usa/200] KF874490, Limnipivirus, Limnipivirus C, fathead minnow picornavirus [fhm/20/il/usa/200] KF306267, Limnipivirus, Limnipivirus B, carp picornavirus [F37/06] JX34222, Limnipivirus, Limnipivirus A, bluegill picornavirus [04-032] KC843627, Potamipivirus, Potamipivirus A, eel picornavirus [F5/05] KJ64698, unassigned, bat picornavirus [BtMf-PicoV-/SAX20] AF327920, Parechovirus, Parechovirus B, Ljungan virus [87-02] HF677705, unassigned, sebokele virus [An/B/227/d] L0297, Parechovirus, Parechovirus A, human parechovirus [Harris] KF006989, unassigned, ferret parechovirus [ferret/mppev/nl] JQ36470, Pasivirus, Pasivirus A [swine/france/20] AB937989, unassigned, crohivirus [shrew/zm54/zambia/202] KC465954, Avisivirus, Avisivirus A [turkey/m76-tuasv/20/hun] KC64703, Avisivirus, Avisivirus A [turkey/usa/in/200] KF979334, unassigned, chicken picornavirus 3 [45C] KT880669, unassigned,chicken picornavirus [Pf-CHK/AsV] KF979333, unassigned, chicken picornavirus 2 [44C] KJ000696, unassigned, aalivirus [duck/gl/2/china/202] KM203656, unassigned, orivirus [chicken/pf-chk/203/hun] KT880667, unassigned, orivirus 2 [Pf-CHK] DQ249299, Avihepatovirus, Avihepatovirus A, duck hepatitis A virus [03D] KC935379, Kunsagivirus, Kunsagivirus A [roller/szal6-kuv/20/hun] unassigned, bat kunsagivirus EU42040, Aquamavirus, Aquamavirus A, seal picornavirus [HO-02-2] KP77040, Ampivirus, Ampivirus A [NEWT/203/HUN] KM87364, unassigned, tortoise picornavirus [44-0] KM87365, unassigned, tortoise picornavirus [203-T4] KM87362, unassigned, tortoise picornavirus [5-04] KM8736, unassigned, tortoise picornavirus [4-04] KM87366, unassigned, tortoise picornavirus [24-4-0] KM87367, unassigned, tortoise picornavirus [5-03] JF973687, Mosavirus, Mosavirus A [mouse/m-7/usa/200] KF95846, Mosavirus,Mosavirus A2 [SZAL6-MoV/20/HUN] JN5727, unassigned, feline picornavirus [356F] 0.77 Page 9 of 0

10 Figure 4 (previous page): Phylogenetic analyses of picornavirus 3CD gene regions using Bayesian tree inference (MrBayes 3.2). 78 sequences were retrieved from GenBank. Presented are GenBank accession numbers, genus names, species names and types. If available, common names and designations of isolates [in square brackets] are given. Yet unassigned viruses are printed in blue. Proposed names are printed in red and indicated by a dot ( ). Numbers at nodes indicate posterior probabilities obtained after 4,750,000 generations. The optimal substitution model (GTR+G+I) was determined with MEGA 5. The scale indicates substitutions/site. Page 0 of 0

a-dS (modules 1 and 11 are required)

a-dS (modules 1 and 11 are required) This form should be used for all taxonomic proposals. Please complete all those modules that are applicable (and then delete the unwanted sections). For guidance, see the notes written in blue and the