Novel Nanotechnology-Based Antiviral Agents: Silver nanoparticle neutralization of hemorrhagic fever viruses

Size: px

Start display at page:

Download "Novel Nanotechnology-Based Antiviral Agents: Silver nanoparticle neutralization of hemorrhagic fever viruses"

Griffin Weaver
5 years ago
Views:

Applied Biotechnology Branch 711 th Human

1 Novel Nanotechnology-Based Antiviral Agents: Silver nanoparticle neutralization of hemorrhagic fever viruses Janice Speshock, Ph.D. Saber Hussain, Ph.D. Applied Biotechnology Branch 711 th Human Performance Wing Air Force Research Laboratory Cleared for public release 88ABW Nanomaterials 2

Silver Nanoparticles UNCOATED POLYSACCHARIDE

Dan Goia, Clarkston University 25 nm 27.

Fever Viruses Arenaviridae South american

2 Silver Nanoparticles UNCOATED POLYSACCHARIDE COATED 10 nm ± 0.13 Dr. Steve Oldenburg, NanoComposix 9.48 ± Dr. Dan Goia, Clarkston University 25 nm ± Dr. Karl Martin, Novacentrix ± 8.38 Dr. Dan Goia, Clarkston University 3 Hemorrhagic Fever Viruses Arenaviridae South american HFV, Lassa Fever, LCMV Enveloped, RNA viruses No effective therapies Candid#1 vaccine 5-35% fatality rate Filoviridae Ebola and Marburg Enveloped, RNA viruses No effective therapies Vaccine in Phase I trials Up to 90% fatality rate 4

Tacaribe Virus New World (Tacaribe) Complex Junin, Machupo, Guanarito, and Sabia Tacaribe virus is a biochemically and serologically close relative of the CDC category A arenaviruses but has a low

3 Tacaribe Virus New World (Tacaribe) Complex Junin, Machupo, Guanarito, and Sabia Tacaribe virus is a biochemically and serologically close relative of the CDC category A arenaviruses but has a low pathogenic potential for humans Experimentally: Cytopathic effect in vero cells lethal meningoencephalitis in mice Arenavirus Experimental Setup 5 2 h.p.i Confocal Microscopy Cell surface expression 12 h.p.i TEM Virus internalization 8 d.p.i Harvest Progeny Virus TCID 50 determination 0 hour 1 h.p.i 4 h.p.i Confocal Microscopy Virus internalization 4 d.p.i qrt-pcr Vero cells 6

4 TCRV Progeny Virus Production Cell Surface TCRV Expression 7 Negative Control 10 nm 50 µg/ml 25 nm 50 µg/ml PositiveControl 10 nm 10 µg/ml 25 nm 10 µg/ml 8

5 Dynamic Light Scattering TCRV Internalization into Vero Cells 9 Untreated TCRV TCRV + 10nm Ag TCRV + 25nm Ag TCRV + 25nm Ag Ag-NP-treated TCRV is internalized into infected Vero cells Ag-NPs and TCRV interact inside the cell lysosomes 10

6 TCRV Internalization into Vero Cells Ag-NPs facilitate uptake of TCRV into Vero cells Nucleoprotein RNA Expression 11 12

7 Mechanism of Ag-NP Inhibition v packaging vrna Filovirus mrna viral protein synthesis 13 qrt-pcr detection of internalized evlps using Gp as a marker Confocal Microscopy of evlp cell surface binding Confocal Microscopy of evlp internalization Cathepsin B and L activity in Vero cells. 14

8 Ebola Virus-Like Particles Cell Surface evlp Expression 15 Vero cells (negative control) 10nm uncoated Ag 10µg/ml 10nm uncoated Ag 50µg/ml 10nm PS-coated Ag 10µg/ml 10nm PS-coated Ag 50µg/ml evlps (positive control) 25nm uncoated Ag 10µg/ml 25nm uncoated Ag 50µg/ml 25nm PS-coated Ag 10µg/ml 25nm PS-coated Ag 50µg/ml 16

evlp Internalization into Vero Cells Negative Control

50µg Cathepsin Activity 17 Bulk and Nano Silver have

9 evlp Internalization into Vero Cells Negative Control 10nm 10µg 10nm 50µg Positive Control 25nm 10µg 25nm 50µg Cathepsin Activity 17 Bulk and Nano Silver have been shown to inhibit enzyme activity. Silver binds readily to thiol groups. Cathepsin B has been shown to have an essential role in Ebola virus replication. Cathepsin L has an accessory role in Ebola virus replication. Cathepsin B L. Jayashankar, Acharya Nagarjuna University, Guntur 18

10 Cathepsin Inhibition Cathepsin B (positive control) 10nm Ag 10µg/ml 10nm Ag 50µg/ml 25nm Ag 10µg/ml 25nm Ag 50µg/ml Cathepsin L (positive control) 10nm Ag 10µg/ml 10nm Ag 50µg/ml 25nm Ag 10µg/ml 25nm Ag 50µg/ml 19 Conclusions Ag-NPs neutralize TCRV infection Decrease in S segment gene expression Decrease in progeny virus production Ag-NPs do not prevent the internalization of TCRV Ag-NPs and TCRV interact inside the cell Mechanism of inhibition occurs between endocytosis and vrna gene production Ag-NPs have a similar effect on evlps Ag-NPs decrease cathepsin activity 20

11 Acknowledgements AFRL/RHPB BIN Group Nanoparticles Dr. Karl Martin (Novacentrix, Austin, TX) Dr. Steven Oldenburg (NanoComposix, San Diego, CA) Dr. Dan Goia (Clarkson University, Center for Advanced Materials Processing, Potsdam, NY) Dr. Laura Braydich-Stolle, Craig Murdock, Eric Szymanski Dr. Amanda Schrand not pictured Ebola virus-like particles Dr. Kelly Warfield (USAMRIID) Dr. Schlager (AFRL/RHPB) and Col. Reilly (AFRL/RH) Funding: DTRA (proposal # _07_AHB_B) & JSTO/DTRA NRC Postdoctoral Fellowship Program (contract #F C-0015) 21 Comments/Questions 22

Viral Hemorrhagic Fevers CDC, AFIP

Viral Hemorrhagic Fevers CDC, AFIP CDC, AFIP Diverse group of illnesses caused by RNA viruses from 4 families: Arenaviridae, Bunyaviridae, Filoviridae, Flaviridae Differ by geographic occurrence and vector/reservoir Share certain clinical