A/equine-1 A/equine-2 (H3N8) H3N8 H7N7 H3N8. Thogovo. Isavirus. * پست الکترونيک نويسندهي مسؤول:

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1 * A/equine-1 A/equine-2 () () Thogovo C B A C Isavirus C B A * پست الکترونيک نويسندهي مسؤول: mhajih@scu.ac.ir

2 (N) (H) A 1A/eq/piague/1/56 A/eq/Jilin/2/89 A/eq/Miami/2/63 Mancini B H5N7

3 Single Radial Haemolysis ELISA Complement Fixation test (SRH) (CFT) Tween-80/Ether α β α HA HA

4 SSS A/eq/progue/1/56 Fisher's Exact Chi- square (A/eq/Miami/1/63 test Eichon OIE BS BS BS

5 >

6 . H 3 N 2 NI(Neuraminidase A 1 A 2 Inhibition) Ataseven

7 2- Ataseven, V.S; and Daly, J.M; Seroepidemiology of Equine Influenza Virus Infection in Turkey. Turk. J. Vet. Anim. Sci; 2007; 31: Blitvich, B.J; Ibarra-Juarez, L.A; Cortes-Guzman, A.J; Root, J.J; Franklin, A.B; Sullivan, H.J; and Fernandez-Salas, I; Seroprevalence of equine influenza virus in northeast and southern Mexico. Vet. Rec; 2010; 166: Chambers, T.M; and Reedy, S.E; Equine Influenza Serological Methods. In: Animal Influenza Virus, Methods in molecular biology, Springer Science + Business Media, New York, Vol:1161 Chapter; 36, 2014; pp: Cowled, B; Ward, M.; Hamilton, S; and Garner, G; The equine influenza epidemic in Australia: spatial and temporal descriptive analyses of a large propagating epidemic. rev. Vet Med; 2009; 9: (Antigenic drift) (Antigenic shift)

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9 Vol.11 / No.2 Autumn-Winter Seroepidemiological study of equine influenza in some provinces of Iran Hematian, M. 1 ; Haji Hajikolaei, M.R. 2* ; Seyfi Abad Shapouri, M.R. 3 ; Bahari, A.A. 4 ; Jahfari Dehkordi, A. 5 ; Ghahghaii, A. 6 ; Ghasemi Seidkolaei, M.B. 7 ; Heidari, M. 1 ; Farhangpajoh, F DVM Graduated Student, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz- Iran. 2. rofessor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz- Iran. 3. rofessor, Department of athobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz- Iran. 4. Associate rofessor, Faculty of ara-veterinary Sciences, Bu-Ali Sina University, Hamadan- Iran. 5. Associate rofessor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran. 6. Assistant rofessor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah- Iran. 7. Iran Veterinary Organization, Mazandaran rovincial Veterinary Service, Mazandaran- Iran. 8. Central Laboratory, Urmia University, Urmia- Iran. Recieved: 19 June 2016 Accepted: 5 March 2017 Summary Influenza is one of the most important equine respiratory diseases. Two subtypes of equine influenza virus A/equine-1 () and A/equine-2 () have been identified. This study was carried out to determine the seroprevalence of equine influenza virus in some provinces of Iran. Blood samples were taken from 290 horses and examined with haemagglutination () test for detection antibodies against to both and subtypes. According to test, antibodies against to were detected in 16(5.52%) horses; however, none of them had antibodies against to. The seroprevalence for in the horses in the Mazandaran, Hamadan, East Azerbaijan and Kermanshah provinces, was 11.32%, 10.72%, 9.53% and 7.9%, respectively. Statistical analysis showed that there was no significant difference between infected horses respected to sex and age. It is concluded that the horses of some partsof Iran are infected only with and therefore, this should be considered in vaccination against to equine influenza virus. Keywords: Equine, Influenza, Seroepidemiology, Iran. * Corresponding Author mhajih@scu.ac.ir 129 Iranian Journal of Vetrinary Clinical Sciences