OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : :23:08 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Equine influenza Address of laboratory: Irish Equine Centre Johnstown Naas Co. Kildare IRELAND Tel.: Fax: address: acullinane@irishequinecentre.ie Website: Name (including Title) of Head of Laboratory (Responsible Official): Sarah McNicholas BBS MSc Chief Executive Officer Name (including Title Position) of OIE Reference Expert: Professor Ann Cullinane Head of Virology Which of the following defines your laboratory? Check all that apply: Other: Registered Charity OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote disseminate diagnostic methods validated according to OIE Stards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Single radial haemolysis Haemagglutination Inhibition Direct diagnostic tests Nationally Internationally RT-PCR Virus Isolation 44 0 ToR 2: To develop reference material in accordance with OIE requirements, implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? NOTE: Currently, there are 22 laboratories that produce Stard Reference Reagents officially recognised by the OIE for 19 diseases/pathogens. Please click the following link to the list of OIE-approved International Stard Sera: If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. 2 OIE Reference Laboratory Reports Activities, 2015
3 Disease Test Available from Equine influenza Haemagglutination inhibition; single radial haemolysis Dr Marie-Emmanuelle Behr-Gross an Directorate for the Quality of Medicines, Council of, 224 avenue de Colmar (entrée rue Schertz), F Strasbourg, France Tel: 33 (0) Fax: 33(0) Type of reagent available Related diagnostic test Produced/ Supply imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries A/eq/Newmarket/2/93 SRH/HI Imported <10mL mL mL >500mL <10mL mL mL >500mL SUDAN 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? OIE Reference Laboratory Reports Activities,
4 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients A/eq/Meath/07 H3N8 Clade 2 Florida sublineage HI, RT-PCR Produced Provided 0 4 ml 2 Americ as Asia Pacific Middle East A/eq/Donegal/09 H3N8 Clade 1 Florida sublineage HI, RT-PCR Produced Provided 0 4ml 2 Americ as Asia Pacific Middle East A/eq/Newmarket/77 H7N7 HI Produced Provided 0 4ml 2 Americ as Asia Pacific Middle East Panel of Specific ferret antisera Virus characterisation Produced Americ as Asia Pacific Middle East Seropositive seronegative equine antisera HI, SRH Produced Provided 0 15 sera - 5ml Americ as Asia Pacific Middle East 4. Did your laboratory produce vaccines? No 4 OIE Reference Laboratory Reports Activities, 2015
5 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disease? No ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month) No. samples received for provision of diagnostic support No. samples received for provision of confirmatory diagnoses MONGOLIA May 11 0 UNITED KINGDOM May Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? OIE Reference Laboratory Reports Activities,
6 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided MONGOLIA Vaccination Remote on site at Irish Equine Centre UNITED KINGDOM Vaccination regimes Diagnosis Remote UNITED STATES OF AMERICA Diagnosis Remote TURKEY Sample collection Diagnosis Remote ISRAEL Vaccine composition Remote BELGIUM Diagnosis Remote CZECH REPUBLIC Vaccine composition Remote ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) OIE Twinning The ultimate aim of the project is that Harbin Veterinary Research Institute will achieve OIE Reference status implement an effective equine influenza surveillance programme. There is currently no OIE reference laboratory for equine influenza outside of North America Harbin Veterinary Institute Epidemiological virological findings during a multiple outbreaks of equine influenza in South America in Virus Characterisation epidemiological investigation Instituto de Virologfa, CICVyA, INTA, Hurlingham, Buenos Aires, Argentina. Assessment of antigenic difference in equine influenza viruses by challenge studies in horses Evaluation of updated vaccines Japan Racing Association 6 OIE Reference Laboratory Reports Activities, 2015
7 ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 The impact of different equine influenza vaccine products other factors on equine influenza antibody levels in Thoroughbred racehorses. Ryan M, Gildea S, Walsh C, Cullinane A. Equine Vet J Nov;47(6):662-6 Epidemiological virological findings during multiple outbreaks of equine influenza in South America in Perglione CO, Gildea S, Rimondi A, Miño S, Vissani A, Carossino M, Cullinane A, Barreguy M. Influenza Other Respir Viruses Sep 25. doi: /irv [Epub ahead of print] The evaluation of twenty two rapid antigen detection (RAD) tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype. Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A. Influenza Other Respir Viruses Nov 14. doi: /irv [Epub ahead of print] The potential impact of a single amino-acid substitution on the efficacy of equine influenza vaccines. Yamanaka T, Cullinane A, Gildea S, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T. Equine Vet J Jul;47(4): Genetic evolution of equine influenza strains isolated in France from 2005 to Legr LJ, Pitel PH, Cullinane AA, Fortier GD, Pronost SL. Equine Vet J Mar;47(2): b) International conferences: 5 "Activities of Equine Group", OFFLU meeting, April 2015, University of Georgia, Athens,USA. Presented by Professor A Cullinane Equine Influenza Vaccination, Closing the Immunity Gap Third International Neglected Influenza Viruses Symposium,Athens,Georgia, USA. April Presented by Professor A Cullinane "Irel's largest equine influenza outbreak since the turn of the century - an in-depth analysis" Third International Neglected Influenza Viruses Symposium,Athens,Georgia, USA. April Presented by Dr Sarah Gildea Searching for evidence with the ubiquitous blood sample. Respiratory Viruses 2015 Serology Serosurveillance, Oxford University, UK. September Presented by Professor A Cullinane. Evidence Based Vaccination Against Equine Influenza Research Update on Equine Viruses, Harbin Veterinary Institute, Harbin, China. October Presented by Professor A Cullinane. Developments in the Diagnosis of Equine Influenza Equine Rhinopneumonitis. Research Update on Equine Viruses, Harbin Veterinary Institute, Harbin, China. October Presented by Professor A Cullinane. The role of an OIE Reference Laboratory. East Asia Regional Meeting on Equine Influenza Control, October, Harbin Veterinary Institute, Harbin, China. October Presented by Professor A Cullinane. OIE Reference Laboratory Reports Activities,
8 c) National conferences: 1 Equine Influenza Vaccination. Equine Health Seminar, Department of Agriculture, Food the Marine, Backweston Laboratory Complex, Dublin. January Presented by Professor A Cullinane. d) Other: (Provide website address or link to appropriate information) 2 OIE Bulletin OIE web site Dedicated space on the OIE website for the ESP recommendation. uenza/ OIE Equine Surveillance Panel on equine influenza vaccine composition - OIE Bulletin No pp Irish Equine Centre website ( ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines as OIE Stards 14. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 1 c) Hs-on training courses: 1 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country b China,Hong Kong, Japan, Mongolia Singapore 10 c Mongolia 1 d Japan 1 ToR 8: To maintain a system of quality assurance, biosafety biosecurity relevant for the pathogen the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Stard? Quality management system adopted ISO17025 Certificate scan (PDF, JPG, PNG format) -The-Irish-Equine-Foundation-Ltd-151T.pdf 8 OIE Reference Laboratory Reports Activities, 2015
9 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited SRH RT-PCR Accreditation body INAB INAB 17. Does your laboratory maintain a biorisk management system for the pathogen the disease concerned? (See Manual of Diagnostic Tests Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location No. Participants International Meeting of Expert Surveillance Panel for equine influenza. Gounalan Pavade March 2015 OIE Headquarters 18 International OIE Twinning Meeting On Equine Virus Research Professor Xiaojun Wang October 2015 Harbin Veterinary Institute 13 International East Asia Regional Meeting on Equine Influenza Control Professor Xiaojun Wang October 2015 Harbin Veterinary Institute Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented OFFLU technical meeting April 2015 University of Georgia, Athens Speaker Activities of Equine Group OIE Reference Laboratory Reports Activities,
10 ToR 10: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant) No. participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. RT-PCR: maintenance of ISO accreditation for Irish Equine Centre, stardisation of assay between reference laboratories. Organiser Participant 2 AHT/IEC SRH:Maintenance of ISO accreditation for Irish Equine Centre, stardisation of assay between reference laboratories. Organiser Participant 2 AHT/IEC HI: Submission of HI for ISO17025 accreditation by Irish Equine Centre stardisation of assay between reference laboratories. Organiser Participant 2 AHT/IEC RT-PCR: Ongoing validation harmonisation of protocols in different laboratories Participant 2 IEC/AHT SRH: Validation of assay for both laboratories Participant 2 IEC/AHT 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories International Surveillance of Equine Influenza Epidemiology, vaccine efficacy, virus characterisation vaccine update Animal Health Trust, Maxwell H.Gluck Equine Research Centre, Free University of Berlin Irish Equine Centre 10 OIE Reference Laboratory Reports Activities, 2015
11 ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries Determining a laboratory s capability to conduct HI, maintenance of existing stard 2 Americas Asia Pacific Middle East Determining a laboratory s capability to conduct RT- PCR, maintenance of existing stard 1 Americas Asia Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) OIE ad hoc Group on International Horse Movement for Equestrian Sport, OIE Headquarters To discuss the member countries comments of the AHG report consider key documents including the HHP health certificate, operationalisation proposals, biosecurity guidelines EDFZs; examine the Terrestrial Code Manual need for revisions; examine research communication needs. Updating of OIE Manual Chapter Remote Equine Influenza 25. Additional comments regarding your report: The OIE Expert Surveillance Panel met with Dr. Marie-Emmanuelle BEHR-GROSS of the an Directorate for OIE Reference Laboratory Reports Activities,
12 the Quality of Medicines HealthCare, to discuss a joint EDQM/OIE study to prepare reference stards. The Irish Equine Centre provided virus as cidate vaccine strain to an immunologicals company in mainl. 12 OIE Reference Laboratory Reports Activities, 2015
OIE Reference Laboratory Reports Activities
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