ANFS 435/635 INTRODUCTION TO ANIMAL VIROLOGY

Transcription

1 ANFS 435/635 INTRODUCTION TO ANIMAL VIROLOGY INSTRUCTOR: Dr. Mark S. Parcells, Professor, Molecular Virology Departments of Animal and Food Sciences and Biological Sciences 052 Townsend Hall, Phone: , (Office); , (Lab 313 Worrilow Hall), Website: Office Hrs: following class and by appointment COURSE TIME AND LOCATION: MWF, 12:30 1:20 PM, 002 Townsend Hall REQUIRED TEXT: Introduction to Modern Virology, Sixth Edition, 2007, N.J. Dimmock, A.J. Easton, and K.N. Leppard, Blackwell Publishing, 516 pages, illustrated, ISBN# ADDITIONAL TEXT AND INFORMATION:, Molecular Biology, T.C. Mettenleiter and F. Sobrino, 2008, Caister Academic Press (selections will be used in course). Additional information will be primary research articles and handouts provided by the instructor. COURSE OBJECTIVES: This course is an introduction to the practical study of viruses that cause disease in animals and humans. The main focus is on viruses that are associated with important diseases in production animals. The student will be introduced to the classification, structure, molecular biology, pathogenesis and host responses to viral pathogens. After having this course, the student will: (1) Understand key elements of primary virus isolation and characterization, (2) Understand the association of viral agents with diseases, and (3) Be able to design experiments dissecting the function of virus gene products. This course addresses University Learning Goals ( 1, 2, 3, 6 and 7. COURSE FORMAT: The course will be comprised of lectures, process-oriented guided-inquiry learning (POGIL) exercises, student presentations, and discussion of current articles. SAKAI-based web material (ANSC435/635 Animal Virology) accompanies the lecture and a multimedia format will be used for lecture presentations. COURSE REQUIREMENTS: This course requires active class participation, the submission of one written paper will be required for undergraduate students (ANFS 435), and two papers will be required for those taking the course at the graduate 1

2 level (2-3 pages/paper). Papers will be succinct synopses of journal articles. Students will be responsible for one presentation and discussion on a primary research article. Specific requirements for these assignments are provided as separate documents. GRADING: Grading will be based on participation in group discussions, three hourly exams, one (or two) papers, one journal article presentation, and one final take-home exam. Hourly exams will be multiple-choice, matching and short answer. The final exam is a take-home, essay-based exam. ACADEMIC HONESTY: The Academic Honesty policy of the University of Delaware will be strictly observed for all work turned in for credit. In particular, no cutting and pasting from published (including website) sources. All uses of published materials must be adequately cited. SCHEDULE OF CLASSES DATE TOPIC READING Helpful Supplementary URLs 9/02/11 Introduction to Virology and Viral Pathogenesis p POGIL /05/11 NO CLASS LABOR DAY 9/07/11 Studying Viruses: Molecular Biological Tools 9/09/11 Studying Viruses: Immunological Tools 9/10/11 Studying Viruses: Virological Assays Handout 1 POGIL 2 Handout POGIL 3 Handouts 3, 4 p Immunological%20tools.pdf

3 /12/11 Virus Structure p POGIL 4 9/14/11 Virus Classification p Handout 5 9/16/11 Virus Entry p Handout 6 9/19/11 Discussion Section First 7 lectures POGIL ruses Exam I Review and Discussion Section 9/21/11 Exam I Basics of Virology and Methods for Studying Viruses 9/23/11 DNA Viruses: Parvo, Circovirus p /26/11 Human Gyrovirus Handout /28/11 ssdna Virus Discussion 9/30/11 dsdna Viruses Herpesviruses 10/3/11 Animal Herpesviruses Marek s Disease Virus Paper 10/5/11 dsdna Viruses Adenoviruses POGIL 6 p Handout 9 Chapter 9 Handout 10 p. 81, 89-90, Discussion Section The Molecular Biology of Animal Herpesviruses Mettenleiter, Keil, and Fuchs

4 10/7/11 dsdna Viruses Poxviruses 10/10/11 dsdna Viruses African Swine Fever Virus 10/12/11 African Swine Fever Virus Paper Handout 11 Handout , , Chapter 10 Handout 10 Handout (Graduate Student Paper I due) 10/14/11 dsdna Viruses Mimiviruses Handout /17/11 Mimivirus Paper Handout /19/11 ssdna and dsdna viruses Discussion POGIL 7 Discussion Section 10/21/11 EXAM II: DNA Viruses 10/24/11 dsrna Viruses Birna, Rota and Reoviruses p Handout /26/11 Rotavirus Article Handout /28/11 dsrna Virus Discussion POGIL 7 Discussion Section 4

5 10/31/11 RNA Viruses (+) Picornaviruses p Handout 17 Chapter data/assets/pdf_file/0004/ /pestiviruses.pdf 11/02/11 RNA Viruses (+) Coronaviruses 11/4/11 RNA Viruses (+) Arteriviruses 11/7/11 RNA Virus (+) Paper p Handout 18 Chapter 4 Handout 19 Chapter 3 PRRSV Transcriptome The Molecular Biology of Mettenleiter, Keil, and Fuchs Arteriviruses Book Chapter /9/11 RNA Viruses (-) Orthomyxoviruses 11/11/11 RNA Viruses (-) Mononegavirales Paramyxoviruses p Handout 20 Chapter 9 p Handout The Molecular Biology of Mettenleiter, Keil, and Fuchs /14/11 RNA retroviruses ALV/ASV 11/16/11 RNA retroviruses HTLV-I, HIV p Handout

6 11/18/11 HTLV-I 11/21/11 EXAM III RNA VIRUSES 11/23/11 Retroid Viruses HBV, Viroids p Handout /25/11 No Class Thanksgiving 11/28/11 Viruses and Cancer DNA Viruses 12/2/11 Student Presentations Student Presentations Handout /5/11 Student Presentations Graduate Student Paper II due (Undergrad Paper I) 12/7/11 Student Presentations Distribution of Final Exams Last Day of Classes 12/14/11 Final Exams due Electronically by 5:00 PM 6