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1 Cries Res 2012;46: DOI: / Published online: My 26, 2012 The bstrcts re vilble free online t 59th ORCA Congress June 27 30, 2012, Cbo Frio, Brzil Abstrcts Contents Session Fluoride nd Erosion Session Microbiology Session Clinicl Dignosis 1 Session Epidemiology Session Cries Risk 1 Session Deminerliztion-Reminerliztion Session Cries Risk 2 Session Clinicl Dignosis Author Index Bsel Freiburg Pris London New York New Delhi Bngkok Beijing Tokyo Kul Lumpur Singpore Sydney

2 Session 1 Fluoride nd Erosion 1 Fluoridted Liquid Dentifrices: Effect on Enmel Deminerlistion nd Alkli-Soluble Fluoride Deposition in vitro A.C. Mglhães *, B.L. Lcerd, S. Chrone, C.A.B. Crdoso, L.F. Grizzo, F. Vilhen, M.A.R. Buzlf usp.br Deprtment of Biologicl Sciences, Buru School of Dentistry, University of São Pulo, Buru, Brzil This in vitro study nlysed the bility of fluoridted cidic or neutrl liquid dentifrices to protect ginst enmel deminerlistion nd on fluoride deposition on enmel. Bovine enmel specimens were rndomly llocted (n = 15) to: experimentl liquid dentifrices with 550 g F/g (ph 4.5); 550 g F/g (ph 7.0); 1,100 g F/g (ph 4.5); 1,100 g F/g (ph 7.0); 5,000 g F/g (ph 4.5); 5,000 g F/g (ph 7.0); plcebo (ph 4.5); plcebo (ph 7.0); nd commercil toothpstes with 550 g F/g (Colgte Bby Brney, ph 7.0); 1,100 g F/g (Crest, ph 7.0); nd 5,000 g F/g (Prevident, ph 7.0). The specimens were subjected to ph-cycles (deminerlistion 6 h/reminerlistion-18h dy) nd treted with one of the dentifrices 2! 15 s/dy for 7 dys. The deminerlistion ws nlysed by surfce nd cross-sectionl hrdness. Additionlly, different bovine enmel specimens (n = 9) were deminerlised for 6 h nd then treted with one of the experimentl dentifrices for 1 min. The deposition of lkli-soluble fluoride ws determined. Dt were tested using ANOVA/Tukey, Kruskll-Wllis/Dunn nd two-wy ANOVA/Bonferroni s test (p! 0.05). All F dentifrices significntly reduced the surfce enmel deminerlistion except the experimentl dentifrice with 550 g F/g (ph 7.0). Regrding the subsurfce deminerlistion, ll F dentifrices were ble to reduce the hrdness loss up to 90 m; however, in deep lyers only the experimentl dentifrices with 1,100 nd 5,000 g F/g were different from plcebo. All experimentl F dentifrices promoted fluoride deposition compred to plcebo. Higher fluoride deposition ws found for 5,000 g F/g compred to the others regrdless of the ph. Considering the experimentl protocol, the effect of fluoridted cidic or neutrl liquid dentifrices to protect ginst enmel deminerlistion in vitro seems to be modulted minly by the fluoride concentrtion. This study ws funded by FAPESP (process 2010/ ). 2 Enmel Crystls of Mice Susceptible or Resistnt to Dentl Fluorosis: AFM Study M.A.R Buzlf, *, C.S. Brbos, A.L. Leite, S. Chng b, J. Liu b, A. Czjk-Jkubowsk c, B. Clrkson b fob.usp.br Deprtment of Biologicl Sciences, Buru School of Dentistry, University of São Pulo, Brzil; b Deprtment of Criology, Restortive Sciences nd Endodontics, University of Michign School of Dentistry, USA; c Deprtment of Conservtive Dentistry nd Periodontics, Poznn University of Medicl Sciences, Polnd This study imed to ssess the surfce roughness nd width of enmel mtrix ptite crystls of mice susceptible (A/J strin) or resistnt (129 P3/J strin) to dentl fluorosis through nlyses by AFM (tomic force microscopy). Smples from the enmel mtrix in the erly stges of secretion nd mturtion were obtined from the incisors of both mice strins fter scrifice. All detectble trces of mtrix protein were removed from the smples by sequentil extrction procedure. The protein-free crystls (n = 13 per strin) were nlysed qulittively in the AFM. Crystl surfce roughness (R) nd width were mesured. The men ( 8SD) R of the crystls of A/J strin ( nm) ws lower thn the one found for the 129P3/J strin ( nm) but the difference did not rech sttisticl significnce (t = 1.187, p = 0.247). Crystl width of the 129P3/J strin ( nm) ws found to be significntly smller (t = 4.013, p = ) thn the sme prmeter mesured for the A/J strin ( nm). The smller width of enmel crystls observed for the 129P3/J strin might help to explin the resistnce of this strin to the development of dentl fluorosis. This study ws prtilly suported by FAPESP (Proc. 2008/ ). * Presenting uthors. E-Mil krger@krger.ch S. Krger AG, Bsel /12/ $38.00/0 This is n Open Access rticle licensed under the terms of the Cretive Commons Attribution- NonCommercil-No- Derivs 3.0 License ( pplicble to the online version of the rticle only. Distribution for non-commercil purposes only.

3 3 Plque Fluoride Levels in Fluoridted nd Non-Fluoridted Communities ccording to Dentifrice Fluoride Concentrtions nd ph, C.A.B. Crdoso *, D.F.B. Mngueir b, S. Chrone, K.P.K. Olympio, L.T. Grizzo, D. Rios c, A.C. Mglhães, F.V. Vilhen, F.C. Smpio b, M.A.R. Buzlf usp.br Deprtment of Biologicl Sciences, Buru School of Dentistry, University of São Pulo, b Helth Sciences Center, Príb Federl University, c Deprtment of Peditric Dentistry, Orthodontics nd Public Helth, Buru School of Dentistry, University of São Pulo, Brzil This study evluted the influence of dentifrice ph nd fluoride concentrtion ([F]) on fluoride (F) uptke in dentl plque, in fluoridted nd non-fluoridted communities. Forty-seven nd fifty-nine 2-to-4-yer-old children living in fluoridted nd in non-fluoridted community, respectively, were rndomly llocted into 3 groups in ech community, ccording to the dentifrice they hd been using for six months: A, liquid dentifrice (LD), 550 ppm F, ph 4.5 (n = 15, non-fluridted/20, fluoridted); B, LD, 550 ppm F, ph 7.0 (n = 16/16); C, LD, 1,100 ppm F, ph 7.0 (n = 16/23). The LDs were loded onto the toothbrush using the drop technique. Plque smples were collected 5 nd 60 minutes fter the lst use of the dentifrices. Plque [F] ws nlyzed with the electrode, fter HMDS-fcilitted diffusion. Dt were tested by 2-wy repeted-mesures ANOVA nd Bonferroni s tests (p! 0.05). Men ( 8 SE, mmol/kg dry weight) plque [F]s were , , for A, B nd C, respectively, 5 min fter the dentifrices use for the fluoridted community. Corresponding vlues for the non-fluoridted community were , , nd , respectively. Sixty minutes fter toothbrushing, corresponding figures were , nd for the fluoridted community nd , nd , for the non-fluoridted community. For the fluoridted community, C ws significntly different from B regrdless the time, while A did not significntly differ from B nd C. For the non-fluoridted community, the differences mong the dentifrices or times did not rech sttisticl significnce. The results suggest tht the reduction of dentifrice ph tends to increse plque F uptke. Additionlly, in the nonfluoridted community, plque F levels did not decrese with time, suggesting tht F from dentifrice occupied more stble binding sites tht were not previously filled by F originted from the diet. This study ws funded by FAPESP (Grnts 2010/ nd 2008/ ). 4 Chemiclly Soluble Fluoride is the Best Indictor of Fluoride Biovilbility from Toothpstes A. F l cã o *, L.M.A. Tenut, J.A Cury fop.unicmp.br Deprtment of Biochemistry, Pircicb Dentl School, University of Cmpins, Brzil Since only biovilble, bsorbble fluoride from toothpstes cn contribute to fluorosis risk, totl fluoride (TF) my not be good predictor of F exposure from toothpstes, especilly in Cbsed toothpstes in which prt of TF is insoluble. Therefore, we evluted if chemiclly totl soluble fluoride (TSF) in toothpstes would be better indictor of biovilble F thn TF. One NF/ silic-bsed (1,100 ppm F) nd one MFP/CCO 3 -bsed (1,450 ppm F) toothpste were tested, either fresh or ged, simulting the decrese of TSF tht occurs with the ltter fter one yer of mnufcturing. In blind, crossover study, 20 dults volunteers ingested both toothpstes in n mount sufficient to provide 49.5 g of TF/kg body weight. Sliv collected for up to 3 h fter ingestion nd 24-h urine were used to indicte F biovilbility. Spermn correltion between the re under the curve of slivry F concentrtion versus time (AUC) nd mount of TF nd TSF ingested by volunteers ws determined. Also, the percentge of F excreted in urine ws clculted in reltion to TF or TSF ingested. TF concentrtions in the fresh nd ged toothpstes were in ccordnce to the expected vlues. All F in the NF/silic formultion, either fresh or ged, ws soluble, but in the MFP/CCO 3 formultion TSF concentrtions were 1, nd , respectively. Dt showed significnt correltion (r = 0.57, p! 0.001, Spermn) between TSF ingested nd AUC but not for TF (r = 0.14, p = 0.20). The expected percentge of F excretion, round 40 50% considering the studied popultion, ws only chieved when TSF ingested ws tken into ccount. In conclusion, the TSF frction nd not TF in toothpstes should be considered in the estimtion of potentil fluorosis risk. Supported by CNPq (130645/2010 9). 5 Dentl Biofilm Fluid Fluoride Concentrtion fter Use of Fluoride Toothpstes by Young Children L.M.A. Tenut *, D.B. Ctni, J.A. Cury fop.unicmp.br Pircicb Dentl School, University of Cmpins, Brzil Low-fluoride toothpste ( g F/g toothpste) hs been recommended for young children s n lterntive to reduce the risk of dentl fluorosis, but its nticries efficcy when compred to the conventionl toothpste (1,100 g F/g) hs been questioned. Since the cpcity to mintin incresed fluoride levels in the orl environment is n indictor of the nticries effect of fluoride toothpstes, the im of this study ws to evlute the vilbility of fluoride in biofilm fluid from these formultions used by children. In double-blind, crossover study, 24 volunteers, 3 4 yersold, brushed their teeth 3! /dy with 0.3 g of the following tooth- Abstrcts: 59th ORCA Congress Cries Res 2012;46:

4 pstes: non-fluoride (negtive control), 500 g F/g or 1,100 g F/g toothpstes (NF/silic). On the lst 3 dys, they were instructed to brush only the occlusl surfces, to llow biofilm ccumultion. On the 7th dy, dentl biofilms were collected from buccl surfces of posterior teeth before nd 30 min fter toothbrushing. The biofilm fluid ws seprted from the solids nd fluoride concentrtion ws determined using n inverted ion-specific electrode. Before toothbrushing, no sttisticl difference in fluoride concentrtion in biofilm fluid ws observed mong tretment groups. Although significntly higher fluoride concentrtions were observed in the fluid 30 min fter brushing with both fluoride toothpstes when compred to the bseline vlues (p! 0.05), fluoride concentrtion ( M, men 8 SD, n = 24) in dentl biofilm fluid fter brushing ws significntly different from the control group ( ) only for the 1100 g F/g toothpste ( ), but not for the low-fluoride toothpste ( ). The findings my suggest rtionle for the conclusions of the systemtic review tht toothpste contining t lest 1,000 g F/g hs greter nticries effect thn those contining g F/g. Supported by CAPES. 6 Urinry Fluoride Excretion by Children Drinking Fluoridted Wter J.A Cury, *, P. Zárte, b, L.M.A. Tenut fop.unicmp.br Pircicb Dentl School, University of Cmpins, Pircicb, b Fculdde de Odontologi, Universidde Federl do Mto Grosso do Sul, Cmpo Grnde, Brzil Drinking wter fluoridtion is considered risk fctor for dentl fluorosis but the biovilble frction of fluoride ingested from diet tht is responsible for its systemic effect hs not been considered. In the present study, fluoride excretion in urine of children living in two communities with distinct levels of fluoride concentrtion in drinking wter ws determined s n indictor of fluoride biovilbility. Twenty-four h urine nd ll diet ingested by 51schoolchildren, ged two to five yers, living in communities with (n = 27) nd without (n = 24) fluoridted wter ( nd ppm F, respectively) were collected for determintion of fluoride concentrtion. Fluoride ws extrcted from dietry products (liquids nd solids) by HMDS-fcilitted diffusion nd its concentrtion wsdetermined using n ion-specific electrode s n indictor of totl fluoride ingested. Fluoride concentrtion in urine ws determined directly using the electrode, fter pproprite buffering with TISAB II. Dily mount of fluoride intke from diet ws nd g F/dy, for communities with nd without fluoridted wter, respectively (p! ). Fluoride excreted in urine ( g F/dy) ws nd , respectively (p = 0.01). The fluoride intke from diet ws 93% lower in the non-fluoridted community but the difference in fluoride excreted ws only 33% smller. Although the results show tht diet hs n importnt contribution to the totl mount of fluoride ingested dily by young children, the findings of urinry fluoride excretion suggest tht only prt of fluoride is biovilble to contribute with dentl fluorosis. Supported by CNPq (151938/2010 5). 7 Fluoride Relesed from Fluoride Vrnish Three Hours fter Appliction P.J. Flnign *, R. Rusin, J.A. Fitch mmm.com 3M ESPE, St. Pul, Minn., USA Fluoride vrnishes prolong contct time between fluoride nd enmel. Vrnishes remin on tooth surfces for 24-hours or more nd ct s slow-relesing reservoir of fluoride. Aim: The im of this study ws to evlute the rte of fluoride relese from three vrnishes: A = 3M TM ESPE TM Clinpro TM WhiteVrnish, B = Premier TM EnmelPro TM Vrnish, C = Colgte TM Durpht TM Vrnish, more thn 3 hours fter ppliction. Experimentl Approch: Vrnish ( g) ws coted on plstic slide (n = 3) over 2-inches2 nd submerged into n gitted TISAB/wter solution contining n ion-specific fluoride electrode. The fluoride in the solution ws mesured every 10 seconds for 15 minutes, nd therefter every minute using Mettler Titrtor. MilliVolts were converted to ppm using stndrdized curve, nd percent fluoride relesed ws clculted bsed on product lbel (5%NF, 22, ppm) nd initil smple weight. Fluoride relesed is reported in percent of totl fluoride content. Results: More fluoride ws relesed in the first minute thn in ny other time intervl: A = 0.27%, B = 8.62%, C = 8.41%. After 3 h, ll vrnishes relesed fluoride ccording to liner model, slope(r 2 ): A = 3.0%/h (0.993), B = 1.1%/h (0.955), C = 0.3%/h (0.995). At 24-hours, most of the contined fluoride ws relesed from vrnishes A nd B, but not C. (A = 83.32%, B = 71.82%, C = 20.22%). Conclusions: This model ssumes ll of the vrnish remins on the tooth surfce. After the 3rd hour, Vrnish A nd B continue to deliver fluoride, vrnish A delivers 11.5% more. In the mouth where sliv continuously bths the vrnish, the bility of vrnish to continuously relese fluoride is beneficil. The vrnish tht relesed low levels of fluoride initilly, exhibited the highest sustined rte of fluoride relese for the long term. This is likely relted to the vrnish s unique prolonged setting. Supported by 3M ESPE, St. Pul, Minn., USA 8 Anticries Potentil of Products Formed/Retined on Enmel by Fluoride Gel or Vrnish A.A. Del Bel Cury *, R.V. Cerezetti, L.M.A. Tenut, T.J. Leitão, J.A. Cury fop.unicmp.br Pircicb Dentl School, University of Cmpins, Brzil The nticries effect of fst-versus slow-recting topicl fluoride products, such s cidulted F (APF) gels nd fluoride vrnishes (FV), hs been demonstrted in clinicl trils but the comprtive mechnism of ction of these products is still to be thoroughly explined. In the present study, the nticries potentil of products formed nd retined on enmel fter APF gel (DFL, 1.23% F, ph 3.5) or FV (Durpht, 2.26% F) ppliction ws evluted using highly-controlled in situ model. Both products were Abstrcts: 59th ORCA Congress Cries Res 2012;46:

5 pplied on enmel blocks ccording to the clinicl recommendtions: APF gel ws pplied for 4 min nd FV ws kept pplied for 24 h before being removed with cetone. Hlf of the blocks were immersed in rtificil sliv for 7 dys to simulte the dissolution of CF 2 -like products ( CF 2 ) tht occurs in vivo. This procedure cused 96% reduction of CF 2 on enmel treted with APF ( to g F/cm2), wheres for the FV the reduction ws 79% ( to g F/cm2). In double-blind, crossover, short-term in situ design, 12 volunteers wore pltl pplinces contining blocks freshly-treted or exposed to sliv, kept in contct with S. mutns test plque nd cid chllenged by sucrose rinse. Untreted blocks were used s control. CF 2 products formed nd retined on enmel by both gels were ble to reduce minerl loss when compred to the control (p! 0.05). Although no significnt difference on enmel deminerliztion mong blocks freshly-treted with both F products ws observed (p ), fter ging the FV-treted blocks presented lower minerl loss thn the APF-treted blocks (p! 0.05). The results suggest tht the nticries effect of slow-recting topicl fluorides is mintined for longer periods of time. Supported by FAPESP (2008/ ). 9 Fluoride Intke from Infnt Foods nd Bottled Wter in the US E.A. Mrtinez-Mier *, A.E. Soto-Rojs, P. Chndrpp, C.B. Buckley, J.L. Steele, J.E. Steinmetz iupui.edu Indin University School of Dentistry, Indinpolis, USA Excessive fluoride intke during enmel formtion hs been ssocited with incresed risk for enmel fluorosis. Food nd beverges hve been identified s significnt sources of fluoride intke for children. The current study ssessed fluoride intke from infnt foods nd bottled wter for one yer old children in the US. FDA totl diet study informtion ws used to determine most frequently consumed mounts nd types of infnt foods nd wter used to reconstitute formul. Fluoride content ws mesured directly (for wter) or using modifiction of the HMDS microdiffusion method (for foods). Men nd stndrd devitions were clculted. Rnges of fluoride intke for different dietry combintions bsed on the typicl US diet nd 50th percentile weight for one yer olds were lso clculted. 458 wter smples from 20 brnds nd 360 infnt food smples from three brnds were collected nd nlyzed for fluoride content. Fruit-bsed foods hd men g F/g; vegetble-bsed hd g F/g; non-chicken bsed hd g F/g; turkey dinners hd g F/g; nd chicken-bsed hd g F/g. Chicken-bsed products hd sttisticlly significnt higher men fluoride concentrtion. For wter smples, fluoride concentrtion rnged from g/ml to g/ml. Vribility between production lots ws not sttisticlly significntly different for foods or wter. Clculted fluoride intke from infnt foods nd bottled wter used to reconstitute formul rnged from 0.07 mgf/kg for dietry combintions using wter nd foods with the highest fluoride content to mgf/kg for clcultions using the lowest fluoride content foods nd wter. Significnt vrition in fluoride intke ws observed with some intke clcultions being higher thn the recommended optiml fluoride intke. This study ws supported by n IUPUI-RSFG grnt for reserch, Delt Dentl Foundtion grnt, nd n IUPUI Signture Center grnt. 10 Fluoride Retention on CO 2 Lser-Treted Enmel fter n Erosive in situ Chllenge M. Esteves-Oliveir, *, K.M. Rmlho b, F. Lmpert, C.P. Edurdo b, C. Apel ukchen.de Deprtment of Opertive Dentistry, Periodontology nd Preventive Dentistry, RWTH Achen University, Achen, Germny; b Deprtment of Restortive Dentistry, University of São Pulo (USP), São Pulo, Brzil The im of the study ws to evlute the fluoride retention on CO 2 lser-irrdited enmel over 5-dys in situ erosive chllenge. 10 volunteers prticipted in this in situ study, with crossover design nd 4 tretments: CO 2 lser irrdition, 0.3 J/cm 2 5 s 226 Hz (L); topicl fluoride tretment AmF/NF, 1.25% F, 3 min (F); fluoride tretment + CO 2 lser (FL); nd no tretment, s negtive control (C). For ech tretment the volunteers used pltl pplinces contining 6 fixed sterilized bovine enmel smples during dy nd night except mels. For erosive deminerliztion the mouth pplinces were immersed extrorlly in 80 ml of 0.05 M citric cid (ph 2.3) for 20 min twice dily. Individul orl hygiene ws performed with fluoride-free products nd without the pplince in situ. Before nd between the tretment periods, 1 week wsh-out period ws included. Two smples were collected from the pplinces for nlysis on dys 1, 3 nd 5 (n = 20/dy/tretment). Acid-extrcted fluoride ws nlyzed using Orion ion-selective electrode nd Orion EA-940 ion nlyzer. Mesurements were performed in triplicte, nd the fluoride concentrtion in the enmel ws determined in g F/cm2. Dt were nlyzed by mens of repeted mesure ANOVA nd post-hoc comprisons (two-sided nd t 5% significnce level). On dy 1 both groups FL ( ) nd L ( ) presented sttisticlly higher fluoride mens thn the control ( ), p! On dy 3 only F group ( ) presented significntly higher mens thn control ( , p = ), nd on dy 5 none of them (p ). CO 2 lser irrdition t 0.3 J/cm 2 combined with previous fluoride ppliction significntly incresed fluoride retention s compred to non-treted nd solely lser-treted enmel up to one dy in n in situ erosion model. Funding: Forschungsgemeinschft Dentl (FGD) nd START Progrm of the Medicl Fculty of the RWTH Achen University (grnts number AZ43/09). Abstrcts: 59th ORCA Congress Cries Res 2012;46:

6 11 Col Drink Modifictions nd Their Effect on Initil nd Prolonged Erosion Lesions: An in vitro Study D. Rios, *, R. Rebelto, M.C. Jordão, M.A.R. Buzlf b, L. Wng c, A.C. Mglhães b, H.M. Honório usp.br Deprtment of Peditric Dentistry, Orthodontics nd Public Helth, b Deprtment of Biologicl Sciences, c Deprtment of Restortive Dentistry, Endodontics nd Dentl Mterils, Buru School of Dentistry, University of São Pulo, Buru, Brzil This in vitro study evluted the effect of exposure to Col on initil nd prolonged erosion lesions. Sixty bovine enmel blocks were rndomly divided into 5 groups (n = 12): RC-regulr col, RCpH ddition of bse to increse regulr col ph, RCAS ddition of sprtme to regulr col, LC-light col, nd LCpH ddition of cid to decrese light col ph. Two-thirds of the blocks surfce ws coted with nil vrnish for reference. The smples were subjected to erosive chllenges (30 ml/smple) for 2 min, 4! / dy. Between the erosive chllenges (2h) nd overnight the smples were immersed in rtificil sliv (30 ml/smple). After 1st experimentl dy, surfce hrdness test ws performed in order to provide the percentge surfce hrdness chnge (%SHC). At the 5th dy, enmel surfce loss ws mesured by profilometry ( m). Dt were tested using ANOVA nd Tukey s test (p! 0.05). Independently of the col modifictions, ll tretments promoted similr hrdness chnge of enmel surfce. RC promoted higher enmel loss ( m) thn LC ( m). The cid ddition to the light col (LCpH: m) significntly enhnced its erosive potentil, which ws comprble to RC. The bse ddition to regulr col (RCpH: m) resulted in similr wer to ER nd EL, respectively. The ddition of sprtme to the regulr col (ERA m) resulted in wer similr to LC, but lso similr to RC. In conclusion, the present in vitro study showed tht the ph modifiction of col drink interferes with the erosive potentil of prolonged enmel erosion, but not with initil erosion. Funded by FAPESP (Proc. 2010/ ). 12 Abrsion Protective Ability of Self-Adhesive Flowble Composites Applied to Eroded Dentin, O. Polydorou *, M. Sprul, K. Heilnd, S. Stmpf b, E. Hellwig uniklinik-freiburg.de Deprtment of Opertive Dentistry nd Periodontology, Dentl School nd Hospitl, University Medicl Center Freiburg, b Institute of Medicl Biometry nd Medicl Informtics, Albert-Ludwigs-University Freiburg, Germny The im of this in-vitro study ws to evlute the brsion protective bility of two different self-etching, self dhesive flowble composites pplied to eroded dentin. 60 smples with n exposed dentin surfce of 5! 5 mm were prepred nd polished up to 1,200 grid. The smples were eroded by using 1% citric cid for 5 min nd rndomly divided mong four groups. Four different mterils were tested: Vertise TM Flow (Kerr), Fusio TM Liquid Dentin (Pentron Clinicl), Sel & Protect (Dentsply DeTrey) nd X-flow using Xeno V s dhesive (Dentsply DeTrey). The dentin surfce of ech specimen ws covered with lyer of the respective mteril (dimeter 5 mm, thickness: 1 mm). Then hlf of the surfce of ech specimen ws covered with tpe nd served s reference. Subsequently, the specimens were exposed to toothbrushing brsion representing six-month clinicl sitution [8,900 movements, lod: 2N, in solution of humn sliv mixed with Elmex Sensitive Professionl TM (GABA) (3: 1)]. Surfce roughness nd substnce loss were mesured using lser profilometry ( m). The smples covered with Sel & Protect did not survive toothbrushing. The X-Flow smples showed higher surfce roughness compred to the other two flowbles. Among the three composite mterils, X-flow showed the lest substnce loss ( m) followed by Vertise Flow ( m) nd Fusio Liquid Dentin ( m). Tukey s Test showed no significnt difference (p ) mong the three tested mterils with respect to substnce loss. The two tested self-dhesive flowbles showed similr performnce compred to the conventionl flowble composite. Within the limits of n in-vitro study it my be concluded they my protect eroded dentin from brsion t lest for more thn six months. 13 The Effect of Dily Fluoride Mouth Rinse ginst Enmel Erosive/Abrsive Wer in situ, K.R. Stenhgen *, L.H. Hove, B. Holme b, A.B. Tveit odont.uio.no Deprtment of Criology nd Gerodontology, Fculty of Dentistry, University of Oslo, b SINTEF Mterils nd Chemistry, Oslo, Norwy It is unknown whether ppliction of fluoride gents on enmel results in lsting resistnce ginst erosive/brsive wer. The im ws to investigte if one dily mouth rinse with sodium fluoride (NF), stnnous fluoride (SnF 2 ) or titnium tetr-fluoride (TiF 4 ) solutions could protect enmel ginst erosive/brsive wer in situ. The study ws prospective, pired, rndomised nd blind study. Sixteen humn molrs were cut into four specimens, ech with one mlgm-filling (reference surfce for mesurements). Specimens from one tooth were mounted together on either left or right buccl side of the crylic mouth pplinces nd worn by eight volunteers for nine dys except during mels nd orl hygiene procedures. Ech morning the specimens were brushed ex situ with tooth brushes nd wter for thirty seconds nd the fluoride solutions (0.4% SnF 2 ph 2.5, 0.15% TiF 4 ph 2.1, 0.2% NF ph 6.5, ll 0.05 MF)) were pplied by pipette (one drop per second) for two minutes. At middy the specimens were etched ex situ for two minutes in 300 ml freshly mde hydrochloric cid (HCl) 0.01 M, rinsed in tp wter nd reinserted in situ. The sme etch procedure ws repeted in the fternoon. Etch depths ( m) were mesured by white light interferometer. A pired t-test ssessed the significnce of differences between the tretment groups. The men etch depths (SD) fter 9 dys of experiment were; SnF ( 81.9) m, TiF ( 8 4.8) m, NF 26.3 ( 8 4.7) Abstrcts: 59th ORCA Congress Cries Res 2012;46:

7 m, control 32.3 ( 8 4.4) m. In conclusion, dily ppliction of SnF 2, TiF 4 nd NF solutions reduced the etch depths by 94%, 90% nd 18% respectively compred with the control (p! 0.05). The study ws funded by Grnts from The University of Oslo. 14 Protective Effect of Fluoride Toothpstes or Fluoride Gel ginst Erosive/Abrsive Wer: An in-situ Study L.H. Hove, *, K.R. Stenhgen, A. Mulic, B. Holme b, A.B. Tveit odont.uio.no Fculty of Dentistry, University of Oslo, b SINTEF Mterils nd Chemistry, Oslo, Norwy Bckground: High nd low concentrted NF toothpstes hve shown cries protective properties, but the preventive effect ginst erosive/brsive enmel wer is uncler. Aim: To mesure the erosion-inhibiting effect of two fluoride toothpstes nd high concentrted SnF 2 gel on the development of erosion-like lesions in double-blind, rndomized in situ study, mesured by White Light Interferometer (WLI). Mterils nd Methods: 16 humn molrs were ech divided into four specimens, mounted on crylic mouth pplinces nd worn by 8 volunteers for 9 dys. The specimens were brushed every dy with fluoride-free toothpste. Tretments; group 1: no tretment (control), group 2: SnF 2 gel 2,500 ppm (5 min) every third dy, group 3: NF toothpste 5,000 ppm 5 min every third dy nd 2 min the other dys, group 4: NF toothpste 1,450 ppm (2 min) dily. In order to mimic gstric reflux, the specimens were etched for 2 min twice dy (0.01 M HCl). Results. The men etch depth in m for the control specimens were 32.9 SD 6.8. The men vlues for the other groups were 22.2 SD 8.4 (group 2), 30.8 SD 7.8 (group 3) nd 31.4 SD 7.7 (group 4). Compred with the control, the SnF2 treted enmel specimens showed significntly lower etch depths. The NF toothpstes (5,000 nd 1,450 ppm F) gve no significnt protective effect, p = 0.2 nd p = 0.4, respectively. Conclusion: Under the present experimentl conditions it cn be concluded tht the ppliction of SnF 2 gel every third dy reduced the etch depth fter cid exposures nd tht the dily ppliction of both low concentrted nd high concentrted NF toothpste gve no protection when exposed to erosive nd brsive chllenges. The study ws funded by grnts from the University of Oslo nd there were no conflicts of interest. 15 IA Survey of Norwegin Dentists Opinions, Knowledge, nd Dignosis of Dentl Erosive Wer A. Mulic, S. Vidnes-Kopperud, A.B. Skre, A. Young *, A.B. odont.uio.no Fculty of Dentistry, University of Oslo, Norwy An electronic questionnire survey ws sent to 1,262 public dentl helth-employed dentists in Norwy in order to investigte their opinions, knowledge nd dignosis of dentl erosive wer. The response rte ws 62% (n = 783, men 43 yers, 65% femle). Nerly ll dentists registered erosive lesions, 51% used specific scoring system: 31% two-grded system (enmel-dentine) nd 14% more detiled system. Erosive lesions were registered on the surfce-level by 54% of respondents nd on the tooth-level by 14%, while 26% registered on the ptient-level. Lesions were reported locted most often on the pltl surfces in upper nterior teeth (79%), nd occlusl surfces of first mndibulr molrs (74%) or first mxillry molrs (32%). Hlf the dentists used clinicl photogrphs for documenttion, 40% never used photos, 60% mde study models nd 34% never mde models. While no gender differences were reported by 36% of dentists, 40% hd the impression tht there were more erosive lesions in mles. Most respondents (77%) usully found probble cuse: crbonted beverges (97%), cidic juices (72%), citric fruits (46%), sports drinks (24%), cidic diet (20%) nd reflux/eting disorders with vomiting (8%). Only 21% of the dentists recorded dietry history, nd 73% reported tht they never mesured sliv secretion in ptients with erosion. Regrding tretment, 78% treted erosive wer ptients themselves, 9% referred to nother dentist/specilist/fculty clinic nd 13% referred only the more severe cses. Conclusion: The survey suggests tht the dentists re reltively up-to-dte regrding the clinicl recording nd dignosis of dentl erosive wer, lthough dietry nd slivry nlyses were not given priority. Documenttion ws not stndrdized, but the mjority of dentists were confident of finding the cuse of the erosive wer nd treting their own ptients. This study ws funded by grnts from the University of Oslo. 16 Ability of Antcid Suspensions to Counterct Enmel Loss by Gstric Acid, C.P. Turssi *, L.M.F. Vinn, A.T. Hr b, F.L.B. Amrl, R.T. Bsting, F.M.G. Frnç gmil.com So Leopoldo Mndic Institute nd Reserch Center, b Orl Helth Reserch Institute, University of Indin, Indinpolis, Ind., USA Current recommended pproches to control dentl erosion cused by gstric cid include the use of neutrlizing gents. Rinsing with over-the-counter ntcid medictions hs been shown to rise introrl ph fter erosive chllenges [Lindquist et l.: Eur J Orl Sci 2011; 119: ]. However, no dt re yet vilble on whether such ntcids cn minimize enmel loss. This in vitro study ws imed to investigte the effect of ntcid suspensions pplied stright fter n erosive event with HCl in controlling enmel loss. Forty-eight slbs from bovine enmel (3! 3! 2 mm) were embedded, flttened nd polished. After reference res hd been creted with dhesive tpe, specimens were divided into 6 groups (n = 8) to be exposed to HCl chllenge (0.01 M ; ph 2) for 2 min nd directly immersed in suspensions contining: (1) mgnesium hydroxide (MH); (2) luminum hydroxide (AH); (3) MH+AH; (4) sodium lginte, sodium bicrbonte nd clcium crbonte (SA+SB+CC); (5) hydrted luminum-mgnesium sulfte (AMS). Suspensions were pplied for Abstrcts: 59th ORCA Congress Cries Res 2012;46:

8 1 min nd then rinsed from the surfce with rtificil sliv. In the control group rtificil sliv rther thn ntcid ws used. Specimens were subjected to further 4 sequences of erosion/ntcid fter which enmel loss ( m) ws mesured with profilometer. Enmel loss (sd) observed s result of pplying MH, AH, MH+AH, SA+SB+CC nd AMS suspensions ws (0.159), (0.217), (0.148), (0.206) nd (0.172), respectively. Wer in control group ws (0.264). ANOVA nd Tukey s test reveled tht ntcid suspensions significntly reduced enmel loss nd tht similr protection ws fforded by ll formultions. The findings indicted tht ntcid suspensions my to some extent counterct enmel loss. FAPESP (grnts #2011/ nd # 2011/ ). 17 Abrsion of Sound nd Eroded Enmel nd Dentin: Effect of Brushing Force of Mnul nd Sonic Toothbrushes A. Wiegnd, *, J.P.M. Burkhrd, F. Eggmnn, M. Roos b, T. Attin zzm.uzh.ch Deprtment of Preventive Dentistry, Periodontology nd Criology, nd b Division of Biosttistics, Institute of Socil nd Preventive Medicine, University of Zurich, Switzerlnd This study imed to determine the brushing forces pplied during in vivo toothbrushing with mnul nd sonic toothbrushes nd to nlyse the effect of these brushing forces on brsion of sound nd eroded enmel nd dentin in vitro. Brushing forces of mnul nd two sonic toothbrushes (Sonic complete, Orl B; Sensonic Professionl, Wterpik; low nd high frequency mode) were mesured in 27 dults prior nd fter instruction of the respective brushing technique nd sttisticlly nlysed by repeted mesures ANOVA. Brushing forces determined in vivo were used for the in vitro experiment, where enmel nd dentin specimens (ech subgroup n = 12) were brushed in n utomtic brushing mchine with the respective brushing forces using fluoridted toothpste slurry. Sound specimens were brushed for 100 min (mnul) or 128 min (sonic). Eroded specimens were brushed for totl of 150 s (mnul) or 192 s (sonic) in cyclic erosion-brsion experiment (30 cycles, ech: 30 s citric cid, ph 2.6, 15 min rtificil sliv, nd 5 or 6.4 s brushing, respectively). Brushing times were djusted ccording to differences in the size of the toothbrush heds nd, thus, to the contct time with the smples surfce. Substnce loss ws determined by profilometry nd sttisticlly nlysed by one-wy ANOVA. Averge brushing force of the mnul toothbrush (1.6 N) ws significntly higher compred to the sonic toothbrushes (0.9 N), which were not significntly different from ech other. Brushing force prior nd fter instruction of the brushing technique ws not significntly different. The mnul toothbrush cused highest brsion of sound nd eroded dentin, while the Sensonic Professionl induced highest brsion of sound nd eroded enmel. Brushing forces of mnul nd sonic toothbrushes re different nd ffect their brsive cpcity. 17A Prevlence, Extent nd Severity of Dentl Erosions in Schoolchildren in Hmburg U. Schiffner, M. Wldmeyer uke.uni-hmburg.de University of Hmburg, Dentl School, Germny The im of the study ws to determine the prevlence s well s the verge extent nd severity of dentl erosions in schoolchildren in Hmburg. The findings should be relted to behviourl dt. 1,580 schoolchildren ged 6 to 15 yers were exmined for dentl erosions by two clibrted investigtors. Erosions were recorded nd rted on scle of 1 to 3, using the index proposed by Lussi. Dt bout orl hygiene nd diet were collected using questionnires for prents nd children. Sttisticl evlution included prevlence, men number of ffected teeth nd the distribution of scores. The prevlence of erosions ws 17.8% nd strongly relted to the childrens ge with rnges from 5.0% in 6-yer-olds to 29.9% in 15-yer-olds. Boys (19.7%) showed higher prevlence thn girls (15.9%, p = 0.051, 2-test). The men number of ffected teeth ws in ll children but in children with erosions which indictes polriztion of the erosion distribution. In totl, 2488 tooth surfces exhibited erosions. The mjority (84.2%) ws restricted to enmel (score 1), nd in only 0.2% dentin ws involved extensively (score 3). Further nlyses were performed for 12-yer-olds (n = 341), which hd n erosion prevlence of 24.9% nd men number of ffected teeth. 36 schoolchildren (10.6%) hd 74.4% of ll erosion prone teeth in this ge group in their mouths. However, in n nlysis of reltions to orl hygiene nd diet, no single vrible could be determined to be significntly relted to prevlence or erosion extent. In conclusion, the study shows considerble prevlence of erosions in schoolchildren, indictes polriztion, but revels low severity. 18 Pnel Study of Risk Indictors for Erosive Tooth Wer in Brzilin Preschool Children C. Murkmi, *, L. Oliveir b, G. Bonini b, T. Ardenghi c, M. Bönecker usp.br Deprtment of Orthodontics nd Peditric Dentistry, School of Dentistry of the University of São Pulo, b School of Dentistry, São Leopoldo Mndic Institute nd Reserch Center, c Federl University of Snt Mri, Rio Grnde do Sul, Brzil Aim: To ssess the prevlence nd risk indictors for ETW in children ged 3 to 4 yers in the city of Didem, São Pulo nd to compre results from 2008 with those found in the ltest survey (2010). Design: A totl of 967 children (2008) nd 995 children (2010) were rndomly selected nd exmined during the Ntionl Children s Vccintion Dy. Sixteen exminers were trined nd clibrted to use modified version of the O Brien index (1994) for ETW lesions nd nutritionl sttus ws ssessed using the WHO criteri (2006). Dt on socioeconomic fctors, nutritionl vribles, dietry hbits nd frequent exposure to gstric Abstrcts: 59th ORCA Congress Cries Res 2012;46:

9 cid were collected using questionnire. Poisson regression model ws used for dt nlysis (p! 0.05). Results: The prevlence of ETW ws similr in 2008 (51.6%) nd 2010 (53.9%) nd most of the lesions found were confined to enmel. There were no significnt ssocitions between ETW nd dentl cries or socioeconomic, environmentl, nd nutritionl vribles. Risk indictors for ETW were dily soft drink intke (p! 0.001), drinking methods cusing prolonged contct with the teeth (p = 0.007) frequent gstroesophgel reflux (p = 0.005), frequent vomiting (p = 0.011), nd n increse in ge (p = 0.003). Conclusions: In conclusion, high nd similr prevlence of ETW ws found in this smple of preschool children in 2008 nd 2010 nd risk indictors included the frequency nd method of soft drink intke, vomiting, reflux nd n increse in ge. Funded by Ntionl Council for Scientific nd Technologicl Development (CNPq) Brzilin Federl Agency for Support nd Evlution of Grdute Eduction (CAPES). 19 Impct of Abrsives nd Chitosn on the Efficcy of Experimentl F/Sn-Toothpstes ginst Erosion/ Abrsion in-vitro O. Grunu *, N. Schlueter, M. Möllers, J. Klimek, C. Gnss dentist.med.uni-giessen.de Deprtment of Conservtive nd Preventive Dentistry, Dentl Clinic, Justus-Liebig-University, Giessen, Germny Tin is notble nti-erosive gent. In toothpstes tin might interct with brsives possibly influencing its efficcy. Chitosn is positively chrged biopolymer with nti-erosive properties nd ble to bind to vrious surfces. The effect of vrying types nd mounts of brsives nd of Chitosn in experimentl F/Sn toothpstes on erosive/brsive tissue loss ws investigted. The study ws conducted in two experiments. In both experiments (E1+E2; 8 groups ech, n = 15) humn enmel specimens were cycliclly deminerlised (10 dys, 0.5% citric cid, ph 2.6; 6! 2 min/dy). Specimens were exposed to toothpste slurries for 2! 2 min/dy nd were brushed for 15 s within the exposure time (2 N, brushing mchine). Negtive control ws erosion only. Substnce loss ws quntified profilometriclly ( m). To bsic formultion (1,400 ppm F, 3,500 ppm Sn) vrying mounts (wt%) of brsives were dded (5, 10, 15, 20% silic or 20% polyethylene, rnge of RDA: 56 81). One formultion contined no brsives; plcebo toothpste contined 20% brsives but no F/Sn. To ll toothpstes in E2 Chitosn ws dded. Tissue loss (E1/E2) fter erosion only ws / In E1/E2 highest tissue loss ws found fter brushing with plcebo ( / ). Tissue loss reduction (%) compred to plcebo rnged between 2% nd 48% in E1 nd between 48 nd 84% in E2. In both experiments efficcy ws not proportionl to silic content. The best efficcy in both experiments ws found with the formultion without brsives (E1: ; E2: ; ech p! compred to plcebo) nd with 20% polyethylene (E1: ; E2: ; ech p! compred to plcebo). Type nd mount of brsives my hve n impct on efficcy beyond brsiveness. Chitosn incresed efficcy independent of the type nd mount of brsives. Supported by GABA Interntionl, Therwil, Switzerlnd. 20 Mode of Action of Experimentl F/Sn-Preprtions: in-vitro nd in-situ Tin Retention on Enmel fter Erosion/Abrsion N. Schlueter *, J. Brinker, J. Klimek, C. Gnss dentist.med.uni-giessen.de Deprtment of Conservtive nd Preventive Dentistry, Dentl Clinic, Justus-Liebig-University, Giessen, Germny Tin nti-erosive properties re bsed on the incorportion of the ion into the dentl hrd tissue. This been shown to occur with highly concentrted mouthrinses in-vitro. However, nothing is known bout the mode of ction of tin under in-situ conditions nd for lower Sn concentrtions s present in toothpstes. In contrst to mouthrinses, toothpstes hve complex composition, contining multitude of ingredients including brsives or efficcy enhncing dditives, e.g. the biopolymer Chitosn. Therefore, their mode of ction might be different to mouthrinses. The retention of tin on nd in humn enmel fter corresponding invitro nd in-situ use of three different F/Sn-contining preprtions ws investigted: one brsive-free gel (3,000 ppm Sn, 1,000 ppm F), two experimentl toothpstes (TP1: 3,500 ppm Sn, 1,400 ppm F; TP2: s TP1 plus Chitosn). All specimens were cycliclly eroded (0.5% citric cid; 6! 2 min/dy) nd treted 2! 2 min/ dy with slurries. In-vitro specimens were brushed for 15 s (2 N, brushing-mchine) within the exposure time. In-situ specimens were introrlly treted nd brushed for 5 s within the 2 min exposure time (2.5 N, powered toothbrush). Tin retention ws mesured by Electron-Dispersive-X-ry-spectroscopy on surfces nd cross sections. Tin content (wt%) on in-vitro surfces ws (gel, TP1, TP2) , , (p! between ll groups), nd on in-situ surfces , , (p! 0.01 between gel nd TP1/TP2). Significnt differences between in-situ nd in-vitro specimens were found for gel nd TP2. Tin incorportion ws deeper under in-vitro thn under in-situ conditions. The use of the gel led to higher tin retention thn the toothpstes, both under in-vitro nd in-situ conditions, perhps due to the lck of brsives. Chitosn hd significnt impct on tin retention under in-vitro, but not under in-situ conditions. Supported by GABA Interntionl, Therwil, Switzerlnd. 21 Role of Abrsives nd Chitosn in Experimentl F/Sn Toothpstes: Tin-Uptke in Enmel fter Erosion/Abrsion in-vitro K. Schulze *, N. Schlueter, M. Jung, J. Klimek, C. Gnss dentist.med.uni-giessen.de Deprtment of Conservtive nd Preventive Dentistry, Dentl Clinic, Justus-Liebig-University, Giessen, Germny Tin nd Chitosn hve promising nti-erosion properties. In toothpstes, however, tin might interct with brsives influencing the efficcy of the preprtion. Chitosn might dsorb to surfces with negtive zet-potentil, thus influencing the tin-uptke Abstrcts: 59th ORCA Congress Cries Res 2012;46:

10 in enmel. This study investigted the tin retention on eroded enmel surfces treted with toothpstes hving different mounts nd types of brsives with nd without Chitosn. Humn enmel specimens (n = 15 ech) were cycliclly deminerlised (10 dys, 0.5% citric cid; 6! 2 min/dy). Fourteen groups were immersed in toothpste slurries for 2! 2 min/dy. Further fourteen groups were dditionlly brushed for 15 s within immersion time (2 N, brushing mchine). Negtive control ws deminerlised only. To the bsic toothpste formultion (3,500 ppm Sn, 1,400 ppm F), with nd without Chitosn, vrying mounts (wt%) of brsives were dded (5, 10, 15, 20% silic or 20% polyethylene, rnge of RDA: 56 81). One formultion contined ctive ingredients only, but no brsives. Plcebo toothpste contined 20% silic, but no ctive ingredients. Tin-uptke (wt%) ws mesured by Energy-Dispersive-X-ry-spectroscopy on surfces. All specimens treted with Chitosn formultions showed significntly higher tin-uptkes (rnge in slurry groups: ; rnge in slurry/brushing groups: ), thn specimens treted without Chitosn (rnge in slurry groups , rnge in slurry/brushing groups: ). Tin-uptke fter polyethylene nd fter silic ws similr. The formultions without brsives, however, showed highest tin retention reching up to threefold higher vlues thn the corresponding toothpstes with brsives (without Chitosn: slurry , slurry/brushing: ; with Chitosn: slurry: , slurry/brushing: ). Though there re effects from mount nd type of brsives, tin retention on eroded enmel surfces depends minly on its presence or bsence, nd is incresed by the ddition of Chitosn. Supported by GABA Interntionl, Therwil, Switzerlnd. 22 Development of n Erosion/Abrsion in-situ Model nd First Efficcy Dt of Experimentl F/Sn/Chitosn Toothpstes A. Tolle *, C. Gnss, J. von Hinckeldey, J. Klimek, N. Schlueter dentist.med.uni-giessen.de Deprtment of Conservtive nd Preventive Dentistry, Dentl Clinic, Justus-Liebig-University, Giessen, Germny There re only few in-situ models with stndrdised introrl brushing. In pilot study simulting the clinicl sitution, such n in-situ model ws developed. In min study, including lrger group of test persons, the efficcy of n experimentl F/Sn/ Chitosn toothpste ws investigted in comprison to other fluoride compounds. Both studies conformed to GCP guidelines, using humn enmel specimens worn in the lower jw (n = 3 on both vestibulr sides ech). The enmel specimens were extrorlly deminerlised (7 dys, 0.5% citric cid, ph 2.6; 6! 2 min/ dy) nd introrlly exposed to toothpste slurries (2! 2 min/ dy). Within the tretment time with the slurries, the specimens on one side were introrlly brushed with powered toothbrush for five seconds (2.5 N). After ech deminerlistion nd slurry exposure, specimens were rinsed with tp wter. Substnce loss ws quntified profilometriclly ( m). Pilot study (n = 10): Four toothpstes were tested (plcebo, two experimentl toothpstes (TP1: AmF/NF/SnCl 2, TP2: AmF/NF/SnCl 2 plus Chitosn; both 1,400 ppm F, 3,500 ppm Sn), nd SnF 2 -contining gel (3,000 ppm Sn, 1,000 ppm F)). In the min study (n = 27) experimentl TP2 ws tested ginst plcebo nd n experimentl NFtoothpste (TP3, 1,400 ppm F). Pilot study: in the plcebo-group, tissue loss ws / (slurry/slurry+brushing respectively); tretment with tin-contining slurries reduced tissue loss (TP / , TP / , gel / , ech p! 0.05 compred to plcebo). Min study: in the plcebo-group, tissue loss ws / After NF, tissue loss ws / nd fter TP / (ech p! 0.05 compred to plcebo; TP2 vs. TP3 p! 0.05). The study model ws ble to differentite effects of ctive gents nd physicl impcts nd showed excellent reproducibility. F/Sn/Chitosn ws superior to NF. Supported by GABA Interntionl, Therwil, Switzerlnd. 23 Interply between Slivry Pellicle Proteins nd Stnnous-Contining Toothpste on Dentl Erosion-Abrsion A.T. Hr *, F. Lippert, D.T. Zero iupui.edu Indin University School of Dentistry, Indinpolis, Ind., USA Stnnous-contining products hve shown n bility to reduce the development of dentl erosion; however, their interction with slivry pellicle proteins is uncler nd ws therefore investigted. Ninety-six bovine enmel nd root dentin specimens (n = 8) were submitted to episodes of deminerliztion (0.3% citric cid, ph 2.6, 5 min, 4! /dy), reminerliztion (in between tretments) nd toothbrushing (2! /dy), for totl of 5 dys. The reminerlizing solutions (S1-S5) contined 1.45 m M C, 5.4 m M PO4, 0.1 M Tris buffer nd sodium zide (S1), with the ddition of 2.2 g/l of Mucin (S2), 2.2 g/l of Csein (S3), 1.1 g/l of Mucin g/l of Csein (S4) or 2.2 g/l of Albumin (S5). Humn sliv (HS) ws included s reference. Toothbrushing (2 min, 45 stk, 150 g) ws performed using slurries of 1,100 ppm F (NF) silic-bsed toothpste suspended in deionized wter (1: 3 w/w), with (TP+Sn) nd without (TP) the ddition of 3,500 ppm Sn (SnCl2). The ph of both slurries ws djusted to 4.5. Surfce loss ws determined using opticl profilometry. Dt were nlyzed by ANOVA nd Fishers LSD tests, t 5% significnce level. When ssocited to TP, HS showed the highest loss, followed by S2. The other protein-contining solutions (S3 S5) did not differ from ech other or from S1. Similr results were observed for dentin. In the presence of TP+Sn, significnt surfce loss reduction ws observed for both substrtes. HS still showed the highest dentl loss, lthough no differences were found mong groups S1 S4. S5 resulted in the lowest enmel loss, not differing only from S2. Similr trend ws observed for S5 on dentin, lthough not s pronounced. Conclusion: The stnnous contining slurry provided strong protective effect ginst dentl erosion-brsion, which seemed to be enhnced by its ssocition to lbumin (S5), especilly on enmel. Abstrcts: 59th ORCA Congress Cries Res 2012;46: