End Sem. Exam. S3-MPN-1P Research Project (P) S3-MPN-2P Research Work Seminar (P)

Transcription

1 M. Pharm. (Pharmacognosy & Phytochemistry) TERMINOLOGY:- S MEANS SEMESTER. MPN MEANS MASTER OF PHARMACOGNOSY & PHYTOCHEMISTRY. L MEANS LECTURE. T MEANS TUTORIAL. P MEANS PRACTICAL. Semester Third Subject code Subject Hours/week Marks L T P Mid Sem. Exam Assign. / Quiz T. A. End Sem. Exam. S3-MPN-1P Research Project (P) S3-MPN-2P Research Work Seminar (P) S3-MPN-3 Medicinal Plant Cultivation and Biotechnology S3-MPN-3P Medicinal Plant Cultivation and Biotechnology (P) Total S3-MPN-4 Elective Total

2 Name of the Course : Research Project (P) Course code: S3-MPN- 1P Duration : 240 Hrs Maximum Marks : 150 Practical : 12 Hrs/week Mid Semester Exam: 40 Marks Assignment & Quiz: 00Marks T. A.: 10 Marks Name of the Course : Research work Seminar (P) Course code: S3-MPN-2P Duration : 120 Hrs Maximum Marks : 150 Practical : 08Hrs/week Mid Semester Exam: 40 Marks Assignment & Quiz: 00 Marks T. A.: 10 Marks

3 Name of the Course : Medicinal Plant Cultivation and Biotechnology Course code: S3-MPN-3 Duration : 40 Hrs Maximum Marks : 150 Theory : Hrs/week Mid Semester Exam:30 Marks Tutorial: 01 Hrs/week Unit -1 Unit -2 Unit -3 Assignment & Quiz:10 Marks T. A.: 10 Marks Content Introduction: Medicinal plant based industry, Export and import of plants, threatened / endangered plants, ecology, biodiversity, geographical variation of plant variety, genotypes. General Aspects involved in cultivation of medicinal plants: GAP, Conservation of medicinal plants: ex-situ and in-situ cultivation: biodiversity law: WTO and TRIPS agreement: CITES Factors involved in production of crude drugs: Exogenous and edaphic factors: mineral supplements: nutrients: growth regulators and inhibitors. Hrs Unit -4 Unit -5 Unit -6 Insect and disease management of medicinal and aromatic plants: Integrated pest plant-based insecticides, and microbial phytotoxins as herbicides, herbal pest management. Cultivation technology, post harvest care and processing of medicinal and aromatic plants: Profile of some high trade value plants: Chirata, Giloe, Gudmar, Isapgol, Jatamansi, Kalmegh, Kesar, Mulethi, Sarpagandha and Tulsi. Introduction to genetics and molecular biology: Plant genome and genomic organization, gene families, genetic regulations in transcription and translation in plants: mutation and mutagenesis, transposable elements, genetic manipulations and plant genetic engineering. 05 Unit -7 Unit-8 Gene mapping and molecular maps of plant genomes: plant chromosome analysis, use of PCR in gene mapping, molecular maps- RFLP, RAPD. Physical maps in-situ hybridization. Tissue culture: Principal and techniques: organogenesis, embryogenesis, micropropagation, haploids through anther, pollen culture, endosperm culture, induction of triploids, nucellus culture, ovary and embryo culture, floral bud culture, shoot primordial, stem and root culture, protoplast isolation, fusion and somatic hybridization. Unit-9 Application of tissue culture in improvement of medicinal plants: Yield improvement, stress tolerant plants, disease resistant plants, pesticide tolerant plants, synthetic seed production, germplasm storage and cryopreservation for conservation of plants. Unit-10 Application of tissue culture in production and enhancement of secondary metabolites: Strategies involving culture conditions, elicitors, precursors, biotransformation, immobilization and hairy root cultures. 05

4 Unit-11 Transgenic plants: Approaches for production of transgenic plants and applications. Unit-12 Enzymes of plant origin: Types, properties, isolation, purification, immobilization, applications and enzyme formulations. Total 40 Text Books: It is strongly recommended that some standard book/s be used for practicals. The choice of book/s is left to the concerned teachers. Reference books : 1. D.R. Murray, Advanced Methods in plant Breeding and Biotechnology CAB International Panima Book Distributors. 2. Dixon, Plant Tissue Culture. IRL Press Oxford Washington DC. 3. Arun and Archana Sharma, Plant Chromosome Analysis, Manipulation and Engineering 1 st Ed. Harwood academic publishers. 4. Murray Moo-Young, Comprehensive Biotechnology. Volume I-IV, Pergamon Press Ltd. 5. R. Ranjan, Transgenic Plants. Agrobotanica. 6. C.K. Atal and B.M. Kapur, Cultivation and Utilization of Medicinal and Aromatic Plants. RRL, Jammu.

5 Name of the Course : Medicinal Plant Cultivation and Biotechnology (P) Course code: S3-MPN-3P Duration : 120 Hrs Maximum Marks : 150 Practical : 08 hrs/week Suggested List of Laboratory Experiments : Mid Semester Exam:30 Marks Assignment & Quiz:10 Marks T. A.; 10 Marks 1. Separation of Solanaceous alkaloids from Belladonna leaf by TLC using hyoscine and hyoscyamine as reference compound. To initiate and develop callus culture and root culture of TrigonellaFoenum- graecum on Murashige and Skoog s and Street &McGroger medium respectively. 2. Determination of Ascorbic acid (Vitamin C) by UV. Spectroscopic method in crude drugs 3. Determination of Hyoscymine/Hyoscine in datura species by UV. Spectroscopic method 4. Quantitative estimation of Reserpine in Rauwolfia serpentine by HPLC method 5. Quantitative estimation of Quinine in Cinchona bark by HPLC method 6. Quantitative estimation of Ephedrine in Ephedra extracts by HPTLC 7. Quantitative estimation of glycyrrhizine in Glycyrrhizaglabra by HPTLC 8. Exercises on Identification of simple Naturally occurring molecules by UV. & IR spectroscopy 9. Exercises on interpretation of at least 5-different known compounds of Natural origin by using spectroscopic data (NMR & MASS). 10. Determination of Microbial load in Crude drugs 11. Separation and identification of aflotoxins in Crude drugs. 12. Preparation of detailed monograph of at least one medicinal plant covering taxonomy physiochemical and pharmacological investigation and its use in traditional system of medicine. Preclinical studies of some herbal extracts like analgesic antiinflammatory and anti anxiety etc

6 Name of the Course : Elective-2 Course code: S3-MPN-4 Duration : 40 Hrs Maximum Marks : 150 Theory : Hrs/week Mid Semester Exam: 30 Marks Tutorial: 01 Hrs/week Assignment & Quiz: 10 Marks T. A. : 10 Marks NOTE: Students have to choose either PART A or PART B. Content PART A: INDIAN SYSTEM OF MEDICINES. S. No Unit-1 Ayurvedic system of Medicines. Unit-2 Siddha system of Medicines. ```` Unit-3 Unani system of Medicines. Unit-4 Homeopathy system of Medicines. Unit-5 Complimentary Medicines. Unit-6 Tribal Medicines. Hrs PART B: INDUSTRIAL PHARMACOGNOSY. Unit-1 Role of Medicinal plants in national economy. Unit-2 Worldwide trade in medicinal plants and derived products. Unit-3 Indian trade in medicinal and aromatic plants. Unit-4 Plant based industry and institutions involved in work on medicinal and aromatic plants in India. Unit-5 Study of infrastructure for different industries. Unit-6 Total 40

7 Text Books: N A References: 1. Ayurvedic Pharmacoepia. 2. Ayurvedic Formulary of India, the Indian Medical Practitioners Co-operative Pharmacy and Store Ltd., IMPCOPS. 3. Hand Book on Ayurvedic Medicines, H. Panda, National institute of Industrial Research, Delhi Ayurvedic system of medicine, 2 nd edition, Kaviraj, Nagendranath Sengupta, Volume 1 st & 2 nd. 5. Siddha Pharmacopoeia by Dr. S. Chidambarathanu Pillai, 1 st Edition. 6. Unani Pharmacopoeia. 7. Homeopathic Pharmacopoeia. 8. Homeopathic Pharmacy an Introduction & Handobook by Steven B. Kayne. 9. Alternative medicines by Dr. K. B. Nangia. 10. Aromatheraphy, Valerie Gennari Cooksley. 11. Indian Herbal Pharmacopoeia, volume 1 st & 2 nd, Indian Drug Manufacturer s Association, Mumbai. 12. British Herbal Pharmacopoeia, British Herbal Medicine Assiociation, 1990, Volume 1 st. 13. GMP for Botanicals Regulatory and Quality issues on Phytomedicine, Business horizons, New Delhi, 1 st Edition, 20. Robert Verpoorte, Pulok K Mukharjee. 14. Screening methods of Pharmacology by Robert turner. 15. Toxiology and Clinical Pharmacology of herbal Products, Melanie Johns Cupp. 16. Textbook of Industrial Pharmacognosy by A. n. Kalia, CBS Publishers and Ditributors, New Delhi. 17. Trease and Evans, Pharmacognosy, Saunders Company, London. 18. Chaudhari R D, Herbal Drug Industry, Eastern Publictions. 19. PDR for Herbal Medicines, 2 nd Edition, Medicinal Economic Company, New Jersey. 20. Herbal drug industry by R. D. Choudhary, 1 st Edition, Eastern Publisher, New Delhi, 1996.