Spin and separate into plastic vial. FREEZE. Transport FROZEN sample from regional office on dry ice. Store and transport sample refrigerated.

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1 07 GUTHRIE CLINIC TEST LIST rev. 5/08 -DEOXYCORTISOL REQUIREMENTS 4 ml Clot Tube SST tubes are unacceptable. PATIENT PREPARATION: First morning sample preferred NOTE: Plasma is also acceptable from EDTA, Sodium heparin and lithium heparin. Spin and separate into plastic vial. FREEZE. Transport FROZEN sample from regional office on dry ice Routine -7 Days Labcorp 7 OH CORTICOSTEROIDS 4 hour Urine Container - Collect with 0 grams boric acid or 5 ml Lavendar N HCl during collection to maintain ph below 7.5. Collect urine with preservative, or keep urine refrigerated during collection if preservative is not used. Store and transport sample refrigerated. Routine -7 Days 4 HR Urine Container HCL Send entire container to the lab. Ship to frozen if preservative is not used 7- HYDROXYPREGNENOLONE 4 ml Clot Tube. Spin and separate into plastic vial : Store and LABCORP: Store and transport FROZEN. Routine -7 Days Labcorp 7- HYDROXYPROGESTERONE 7-KETOSTEROIDS 4 ml Clot Tube SST tubes are unacceptable Alternative specimens: Plasma collected in an EDTA (lavender-top), EDTA (royal blue-top), sodium heparin (green-top) or lithium heparin (green-top) tube 4 Hour Urine Container - Collect with 0 grams boric acid or 30 ml Lavendar N HCl during collection to maintain ph below 7.5. Submit in a plastic, leak-proof container. Spin and separate into plastic vial Store and Store and transport sample refrigerated. Send entire container to lab Routine -7 Days Labcorp Routine -7 Days 4 HR Urine Container HCL Test includes 7-ketosteroids and creatinine 5' NUCLEOTIDASE 5-HIAA, 4 HR URINE : 4 Hour HCl Urine Container Store and transport sample refrigerated. LabCorp: 4 Hour Urine Plain Container Send entire container to lab Patient Preparation: Patient should avoid food high in indoles: Avocados, banana, tomato, plum, walnut, pineapple, and eggplant. Routine -7 Days Labcorp Routine -7 Days Labcorp 4 HR Urine Container 4 HR Urine Container HCL ABG (CH) Collect 4-hour urine with 5 ml of Lavendar N HCl to maintain a ph below 3. Urine without preservative is acceptable if ph is below Lavendar and the sample is shipped frozen. Keep urine refrigerated during collection if preservative is not used. Record 4-hour urine volume on test request form and urine vial. Arterial blood samples are collected by Respiratory Therapy in heparinized syringes. Refer to CH739-4 for collection procedure. All specimens are transported in a biohazard bag. Plastic syringes are kept at room temperature and analyzed within 30 minutes of collection. STAT Routine ABG (RPH/TCH) Anaerobic sample that is obtained in a heparinized blood gas syringe. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. STAT Routine RPH RT Lab TCH RT Lab Sterile Syringe Sterile Syringe

2 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ABG W/ K, HGB, IONIZED CA (PULM) ABG W/CRITICAL CARE PANEL (PULM) ABG W/HGB (PULM) ABG W/IONIZED CA, LACTIC ACID (PULM) ABG W/IONIZED CALCIUM (PULM) ABG W/K (PULM) ABG W/K HGB (PULM) ABG W/LACTIC ACID (PULM) REQUIREMENTS Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. STAT Routine STAT Routine STAT Routine STAT Routine STAT Routine STAT Routine STAT Routine STAT Routine RPH RT Lab Sterile Syringe RPH RT Lab TCH RT Lab Sterile Syringe Sterile Syringe RPH RT Lab Sterile Syringe RPH RT Lab Sterile Syringe RPH RT Lab Sterile Syringe RPH RT Lab Sterile Syringe RPH RT Lab Sterile Syringe RPH RT Lab Sterile Syringe

3 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ABG WITH CARBOXYHGB (PULM) ABNORMAL COAG INVESTIGATION (QUEST) REQUIREMENTS Anaerobic sample that is obtained in a heparinized blood gas syringe. Collect: Blue 4.5ml citrate tubes This test includes both PT and PTT testing Correct ratio of blood to citrate is critical. Avoid hemolysis. Tubes must be filled completely and mixed gently 8 times. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Specimens are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. Spin and separate plasma into separate plastic vials. FREEZE both samples. Transport FROZEN samples from regional office on dry ice. Samples are to be kept at room temperature and centrifuged to obtain platelet poor plasma within four hours of collection. The centrifuged sample must be tested within 4 hours of collection. If testing will be delayed more than 4 hours, separate the platelet poor plasma into a labeled polypropylene tube and freeze. Spin time to obtain platelet poor plasma depends on the centrifuge speed. RPM Spin Time for PPP minutes 3Lavendar 00 0 minutes 4000 Lavendar minutes minutes STAT Routine RPH RT Lab TCH RT Lab Sterile Syringe Sterile Syringe Routine -7 Days Lt Blue Top-Citrate ABNORMAL COAG INVESTIGATION PT (LABCORP) Collect: Blue 4.5ml citrate tubes This test is for specimens that are being sent to Labcorp. When an Abnormal Coag Investigation is requested, both the ACI PT and PTT tests are automatically ordered. Spin to obtain platelet poor plasma and separate plasma into a labeled polypropylene tube. FREEZE. Transport FROZEN samples from regional office on dry ice. Spin time to obtain platelet poor plasma depends on the centrifuge speed. RPM Spin Time for PPP minutes 3Lavendar 00 0 minutes 4000 Lavendar minutes minutes Routine -7 Days Labcorp Lt Blue Top-Citrate 3

4 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ABNORMAL COAG INVESTIGATION PTT (LABCORP) REQUIREMENTS Collect: Blue 4.5ml citrate tubes This test is for specimens that are being sent to Labcorp. When an Abnormal Coag Investigation is requested, both the ACI PT and PTT tests are automatically ordered. Spin to obtain platelet poor plasma and separate plasma into a labeled polypropylene tube. FREEZE. Transport FROZEN sample from regional office on dry ice. Spin time to obtain platelet poor plasma depends on the centrifuge speed. Routine -7 Days Labcorp Lt Blue Top-Citrate RPM Spin Time for PPP minutes 3Lavendar 00 0 minutes 4000 Lavendar minutes minutes ABORH Pink Lavendar ml EDTA preferred Whole blood required. Mix sample thoroughly by gentle inversion 8 times to avoid clotting. Do not spin. Stable refrigerated up to 7 STAT hours. Routine ACETAMINOPHEN 4 ml Spin and separate into separate vial. Store and SST tubes are unacceptable. ACETONE, SERUM PATIENT PREPARATION: Prep the venipuncture site with an Iodine prep. Lt Green 4.5 PST is used as an alternate specimen. ACETYLCHOLINE RECEPTOR, BINDING ACETYLCHOLINE RECEPTOR, BLOCKING ACETYLCHOLINESTERASE, Isolation Tube AMNIO FLD 3ml of amniotic fluid are required ACTH ADENOVIRUS ANTIBODIES AEROBIC (WOUND) CULTURE WITH GRAM STAIN Indicate if the patient is immune suppressed. Label with specimen SOURCE. Store and transport at ROOM TEMPERATURE. Lavender 3 ml EDTA Spin and separate plasma PATIENT PREPARATION: Patient should be fasting. IMMEDIATELY. FREEZE. Store and Collect between 7 am & 0 am. If drawn any other time, the transport sample on dry ice. reference ranges do not apply. Patient Prep: Minimum fast of 4 hours. Non-Spifasting specimens cause method interference. transport and do not separate. Store and refrigerated.. Remove surface exudate by wiping with sterile saline or 70% alcohol.. Aspirate if possible, or pass a swab deep into the lesion and firmly sample the lesion's advancing edge. 3. A sample from the base of the lesion and a sample from the abscess wall are most productive. 4. Tissue or fluid is always superior to a swab specimen. Reference Range: No aerobic growth <= 48 hours, Room Temperature STAT Routine STAT Routine Hours Hours Hours Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Guthrie Clinic Blood Bank Corning Hospital Blood Bank Troy Hospital Blood Bank Guthrie Clinic Lab Guthrie Clinic Lab Pink EDTA Pink EDTA Pink EDTA Isolation Tube Isolation Tube Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Sterile Swab Rejection Criteria: Received frozen Received in viral transport device Improper specimen collection, storage, and transport ALBUMIN Routine -7 Days Guthrie Clinic Lab 4

5 07 GUTHRIE CLINIC TEST LIST rev. 5/08 REQUIREMENTS ALBUMIN FLUID White Fluid Tube List specimen SOURCE on the container. Store and Routine -7 Days Guthrie Clinic Lab ALCOHOL LEVEL, MEDICAL Gray 4 ml Fluoride/Oxalate DO NOT use alcohol or alcohol gray 4ml fluoride/oxalate tubes Routine -7 Days Guthrie Clinic Lab containing solution for skin prep. Use a non-alcohol solution required. Do not spin or separate, such as betadine. whole blood required. The lab will centrifuge the sample and remove plasma for testing. White Body Fluid Tube White Body Fluid Tube Gray Top Tube Gray Top Tube ALCOHOL,ETHYL PAIN PANEL 0 ml urine in a sterile container Store and Routine -7 Days ALDOLASE spin and do not separate. Hemolyzed specimens are not acceptable. Store and Routine -7 Days Labcorp ALDOSTERONE 4 ml Clot Tube PATIENT PREPARATION: Patient must remain in an upright, sitting position for at least 30 minutes prior to specimen collection. ALDOSTERONE, 4 HR URINE : 4 Hour Urine Container - Collect urine with 0 grams of boric acid to maintain ph below 7.5. Refrigerate during and after collection. Sample without preservative is acceptable if kept refrigerated during collection. It is acceptable to add preservative after collection if urine was refrigerated during collection. Labcorp: 4 Hour Urine Container with no preservative Spin and separate into a plastic vial. Store and Store and transport sample refrigerated. Send entire container to the lab ALKALINE PHOSPHATASE ALKALINE PHOSPHATASE, BONE SPEC ALKALINE PHOSPHATASE, ISOENZYMES ALPHA MACROGLOBULIN ALPHA FETOPROTEIN AMNIOTIC PATIENT PREPARATION: Overnight fast required Avoid hemolysis. Overnight fasting is preferred. Collect cc (discard first cc) amniotic fluid into a small vial. Label with patient name, B# and specimen SOURCE. Gestational Age is Required requires a signed consent form which maybe obtained by clicking the link below. Minimum sample: ml amniotic fluid Spin and do NOT separate. Store and Store and transport at ROOM TEMPERATURE Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp 4 HR Urine Container 4 HR Urine Container ALPHA FETOPROTEIN MATERNAL SCREEN The MSAFP test in pregnant women should be performed between 5.0 and.9 weeks gestational age, although the optimal period is 5.0-Lavendar.9 weeks. This time frame allows sufficient opportunity for further diagnostic studies if the initial MSAFP results are abnormal. Specimens submitted before 5.0 weeks or after.9 weeks gestation cannot be properly evaluated. This test is used for screening of open neural tube defects. Minimum sample ml Routine -7 Days Labcorp ALPHA FETOPROTEIN MATERNAL SCREEN 3 Test includes alpha-fetoprotein, human chorionic gonadotropin, unconjugated estriol. Provides risk assessment for fetal Down syndrome, trisomy 8 and open neural tube defects. Test during the second trimester between 4.0 and.9 weeks gestation. Minimum sample: ml Routine -7 Days 5

6 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ALPHA FETOPROTEIN MATERNAL SCREEN 4 REQUIREMENTS Test includes alpha-fetoprotein, human chorionic gonadotropin, unconjugated estriol and Inhibin A. Provides risk assessment for fetal Down Syndrome, trisomy 8 and open neural tube defects. Test during the second trimester. Minimum sample ml serum Routine -7 Days Labcorp ALPHA FETOPROTEIN, MATERNAL SCREEN 5 ALPHA SUBUNIT ALPHA- ANTITRYPSIN PHENOTYPE Test includes alpha-fetoprotein, human chorionic gonadotropin, unconjugated estriol, inhibin A and h-hcg. Provides risk assessment for fetal Down Syndrome, trisomy 8 and open neural tube defects. Test during the second trimester. MInimum sample:.5 ml 4 ml Routine -7 Days : Spin and separate. Store and Labcorp: Spin and separate. Store and transport FROZEN. Routine -7 Days Labcorp Spin and do not separate. : Store and Labcorp: Store and transport at room temp Routine -7 Days Labcorp ALPHA--ACID GLYCOPROTEIN 4 ml Clot Tube Spin and separate into a plastic vial. Store and ALPHA--ANTITRYPSIN ALPHA--ANTITRYPSIN Lavender EDTA tubes Do not spin or separate. Store and MUTATION 5 ml whole blood is required. A signed informed consent form is required to perform testing Minimum sample: ml whole blood ALPHA--ANTITRYPSIN, 0 grams of a random feces collected and submitted in a STOOL plastic, leak-proof container. FREEZE. ALPHA-FETOPROTEIN MARKER ALT (SGPT) ALUMINUM Royal Dark Blue Lavendar ml No Additive SERUM Trace Metal PATIENT PREPARATION: Patient should refrain from taking antacids containing aluminum compounds at least three days prior to sample collection Draw one test tube of blood and DISCARD! Draw a second test tube. Allow the serum to clot in an upright position. Spin and separate serum into a trace element container within hour of collection. DO NOT use a pipet. Serum must be carefully poured off to avoid trace element contamination Minimum sample: ml Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Transport frozen sample on dry ice. Routine -7 Days Labcorp Routine -7 Days Labcorp STAT 45 Routine 4 Hours Store and Routine -7 Days Labcorp Guthrie Clinic Lab Royal Blue Plain Royal Blue Plain AMIKACIN AMIKACIN PEAK 4 ml Clot Tube RANDOM: Specimen can be drawn at any time. 4 ml Clot Tube PEAK: Draw one hour after intramuscular dose or 30 minutes after the completion of a 30 minute intravenous infusion. Spin and separate into plastic vial. SST tubes are NOT acceptable. Store and Spin and separate into plastic vial. SST tubes are NOT acceptable. Store and Routine -7 Days Guthrie Clinic Lab Routine -7 Days Guthrie Clinic Lab 6

7 07 GUTHRIE CLINIC TEST LIST rev. 5/08 AMIKACIN TROUGH AMINO ACID QUANT,URINE REQUIREMENTS 4 ml Clot Tube TROUGH: Draw just before next dose. NOTE: Please indicate Peak / Trough / Random on request and on the specimen container Urine Container Plain, Random Provide patients age, sex, brief clinical history, tentative diagnosis, therapy over the last 3 days (drugs, x-rays, diet) Spin and separate into plastic vial. SST tubes are NOT acceptable. Store and Routine -7 Days Guthrie Clinic Lab Store and Routine -7 Days Labcorp Sterile Cup Sterile Cup AMINO ACIDS, PLASMA AMIODARONE LEVEL AMITRIPTYLINE LEVEL AMMONIA AMNISURE ROM Green 0 ml Sodium Heparin PATIENT PREPARATION: A 4 hour fast required Provide patient age, sex, clinical history, diagnosis, and therapy over the last 3 days (drugs, x-ray, diet). 4 ml Clot Tube PATIENT PREPARATION: Collect no SOONER than hours after last dose. 4 ml Clot Tube Test includes Nortriptyline Level Minimum sample: ml Sayre/Troy: Green Yellow Lithium Heparin PST Corning: Lavendar 3 ml Place sample on ice IMMEDIATELY after collection and deliver IMMEDIATELY to the Laboratory. The plasma must be remove from the cells within 5 minutes of collection. Regional Offices: Spin and separate plasma -freeze sample immediately. Special Collection Kit Required. Swabs and solvent are stored in the maternity unit. Call x-5lavendar 80 to request a kit. PATIENT PREPARATION: Women with moderate to gross vaginal bleeding are NOT tested. Blood will cause false test results. Spin and separate into plastic vial. FREEZE immediately Spin and separate into separate vial. Store and SST tubes are unacceptable Spin and separate serum as soon as possible into a separate vial. Store and SST tubes are unacceptable. SAYRE,CORNING, TROY CAMPUS: Place sample on ice IMMEDIATELY after collection and deliver IMMEDIATELY to the Laboratory. REGIONAL OFFICES:. Specimens should be put on ice immediately after collection.. Centrifuge specimen. 3. Aliquot plasma into plastic transport tube. Keep on ice. 4. Freeze plasma immediately. 5. Transport to the laboratory frozen. Submit the specimen to the lab within 30 minutes. Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Routine -7 Days Guthrie Clinic Lab Green Sodium Heparin Green Sodium Heparin Amnisure Amnisure AmniSURE must not be used earlier than Lavendar hours after the removal of any disinfectant solutions or medications from the vagina. Submit the specimen to the lab within 30 minutes. AMOEBIC ANTIBODY, IGG Spin and do NOT separate. Store and AMPHETAMINE CONF, SERUM : 0 ml Clot Tube Labcorp: 7 ml whole blood collected in gray top tube : Spin and separate into plastic vial. Store and Labcorp: Do not spin or separate. Store and Routine -7 Days Labcorp Routine -7 Days Labcorp Gray Top Tube AMPHETAMINE CONFIRMATION 0 ml urine collected in a urine container with temperature strip Store and Routine -7 Days Labcorp AMYLASE Routine -7 Days Guthrie Clinic Lab AMYLASE 4 HR URINE (QUEST) 4 Hour Urine Plain Container Transport entire container to lab. Store and Routine -7 Days 4 HR Urine Container AMYLASE BODY FLUID White Fluid Tube List specimen SOURCE on the container. Store and STAT Routine 45 4 Hours Guthrie Clinic Lab White Body Fluid Tube White Body Fluid Tube AMYLASE ISOENZYMES Routine -7 Days Labcorp 7

8 07 GUTHRIE CLINIC TEST LIST rev. 5/08 AMYLASE, PERITONEAL FLUID AMYLASE,RANDOM (REF LAB) REQUIREMENTS Isolation Tube Notes:List specimen SOURCE on the container. Random urine-transfer to 0ml urine tube for transport. Store and Routine -7 Days Labcorp Transfer to 0ml urine tube for Routine -7 Days Labcorp transport. Store and transport refrigerated Routine -7 Days Labcorp White Body Fluid Tube White Body Fluid Tube Yellow Urine Conical Tube ANA (ANTINUCLEAR ANTIBODIES) (REF LAB) ANA TITER (REF LAB) Gold tube Store and Routine -7 Days ANAEROBIC CULTURE Routine -7 Days Guthrie Clinic Lab Anaerobic Specimen Collector W/GRAM STAIN. Remove surface exudate by wiping with sterile saline or 70% alcohol.. Tissue or fluid is always superior to a swab specimen. 3. Aspirate material with needle and syringe. 4. Aseptically transfer all material into anaerobic transport device. (Fluid volume should be >= ml. Syringe need to have needle removed.) All ANAEROBIC cultures have an AEROBIC culture performed automatically. ACCEPTABLE SPECIMENS for ANAEROBIC CULTURE: see individual culture & sensitivity source listing for further instruction. Abscess aspirates Blood Culture Body fluids Bone *Bone Marrow Core Samples Bronchial brush CSF (cerebrospinal fluid) Deep wounds Genital specimens from culdocentesis or abscess Suprapubic bladder aspiration Tissue Transtracheal aspration Reference Range: No growth aerobic/anaerobic Store and transport at room temperature up to 7 hours. If not received in anaerobic transport device, acceptable storage/transport <= 4 hrs at RT ANDROSTANEDIOL GLUCURONIDE ANDROSTENEDIONE ANGIOTENSIN CONVERT ENZYME CSF ANGIOTENSIN CONVERTING ENZYME ANGIOTENSIN II ANSER ADA/IFX Rejection Criteria: Improper specimen collection, storage, and transport 4 ml Clot Tube SST tube is also acceptable Alternate specimen: ml lav EDTA plasma 4 ml Clot Tube An early morning specimen is preferred. Spinal Fluid Container - Collect ml of CSF. Label with specimen SOURCE Minimum sample: 0. ml Minimum sample: ml serum Lavender 3 ml EDTA Minimum sample: ml plasma 4 ml Clot Tube Signed consent form is required to perform testing. Specify ADA or IFX test on requisition based on the medication patient is taking. Spin and separate. Store and transport refrigerated Spin and separate. Store and transport refrigerated. FREEZE immediately. Store and transport on dry ice. Spin and do not separate. Centrifuge within hour of collection. Store and Spin and separate. FREEZE. Transport frozen sample on dry ice Spin and separate into plastic vial. Store and Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab CSF Container CSF Container 8

9 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ANTENATAL RHOGAM SCREEN REQUIREMENTS Pink Lavendar ml EDTA preferred Whole blood required. Mix sample thoroughly by gentle inversion 8 times to avoid clotting. ANTI ADRENAL ANTIBODY WITH REFLEX TITER ANTI CARDIOLIPIN AB IGA, IGG, IGM Minimum sample: ml ANTI CARDIOLIPIN ANTIBODY IGA Minimum sample: ml ANTI CARDIOLIPIN ANTIBODY IGG Minimum sample: ml ANTI CARDIOLIPIN ANTIBODY IGM Minimum sample: ml ANTI DNA, DOUBLE STRANDED ANTI DNA, DOUBLE STRANDED (REF LAB) ANTI DNASE B ANTIBODY serum ANTI GLIADIN ANTIBODY PANEL Minimum sample: ml ANTI GLOMERULAR BASEMENT MEMBR Minimum sample ml ANTI HISTONE ANTIBODY serum ANTI HU ANTIBODY WITH REFLEX TO TITER ANTI JO- ANTI MYELOPEROXIDASE AB ANTI NEUTROPHIL CYTO ANTIBDY Minimum sample: ml ANTI PROTEINASE 3 ANTIBODY PATIENT PREPARATION: Overnight fast is preferred If this test is positive a Western Blot confirmation and titer are performed automatically Avoid hemolysis Minimum specimen: 0.5 ml Do not spin. Store and ship at room temperature or refrigerated up to 7 hours Spin and do not separate. Spin within hour of collection. Store and transport refrigerated Avoid hemolysis ANTI SPERM ANTIBODY, SERUM ANTI STREPTOLYSIN O ANTI TSH ANTIBODY ANTI YO AB (PURKINJE CELL) W REFLEX PATIENT PREPARATION: Overnight fast preferred If the test is positive a titer will be performed automatically Routine -7 Days Guthrie Clinic Blood Bank Corning Hospital Blood Bank Troy Hospital Blood Bank Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Pink EDTA Pink EDTA Pink EDTA Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Routine -7 Days 9

10 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ANTIBODY SCREEN ANTIBODY TITER REQUIREMENTS Pink Lavendar ml EDTA preferred Whole blood required. Mix sample thoroughly by gentle inversion 8 times to avoid clotting. Pink Lavendar ml EDTA preferred Whole blood required. Mix sample thoroughly by gentle inversion 8 times to avoid clotting. Do not spin. Stable refrigerated up to 7 hours. Do not spin. Store and ship at room temperature or refrigerated up to 7 hours ANTIMITOCHONDRIAL AB (REF LAB) ANTI-PARIETAL CELL AB Avoid hemolysis If test is positive a titer will be performed automatically Routine -7 Days Guthrie Clinic Blood Bank Corning Hospital Blood Bank Troy Hospital Blood Bank Routine -7 Days Guthrie Clinic Blood Bank Corning Hospital Blood Bank Troy Hospital Blood Bank Routine -7 Days Labcorp Routine -7 Days Labcorp Pink EDTA Pink EDTA Pink EDTA Pink EDTA Pink EDTA Pink EDTA ANTIPHOSPHOLIPID AB PANEL Blue.7 ml Sodium Citrate Centrifuge test tube for 5 minutes within Lavendar 0 minutes of collection Using a plastic pipet, remove plasma avoiding WBC/platelet buffy layer Place plasma into a plastic vial and centrifuge a SECOND time Transfer platelet-poor plasma into a new plastic vial. Store and Routine -7 Days Lt Blue Top-Citrate ANTI-SMOOTH MUSCLE ANTIBODY (REF LAB) ANTITHROMBIN Blue Citrate.7 ml Correct ratio of blood to citrate is critical. Avoid hemolysis. Tube must be filled completely and mixed gently 8 times. Routine -7 Days Labcorp Separate and ship Frozen Routine -7 Days Guthrie Clinic Lab Lt Blue Top-Citrate Samples are kept at room temperature and centrifuged to obtain platelet poor plasma within four hours of collection. The centrifuged sample must be tested within 4 hours of collection. If testing is delayed more than 4 hours, separate the platelet poor plasma into a labeled polypropylene tube and freeze. Spin time to obtain platelet poor plasma depends on the centrifuge speed. RPM Spin Time for PPP minutes 3Lavendar 00 0 minutes 4000 Lavendar minutes minutes 0

11 07 GUTHRIE CLINIC TEST LIST rev. 5/08 AP/SURGICAL SPECIMEN LABEL REQUIREMENTS Surgical/anatomic pathology services are provided by the Guthrie Clinic Laboratory and oratory. The Anatomic Pathology department is open Monday through Friday, 8 a.m. to 5 p.m. All specimens picked up or received after 4 p.m. or on weekends are processed the next working day. A pathologist on-call can be contacted through the Guthrie telephone operator. Immediately place biopsy specimen in a tightly secured container with 0% neutral buffered formalin. Sample must be completely submerged in formalin, do not allow the specimen to dry. Use a separate container for each separately identified specimen. Do not force a large specimen into a small container. Formalin must surround the tissue. Label samples noting the site and source of each specimen. Ink pens, not markers, must be used to complete the labels. Formalin washes away writing performed with marker. Place the labels on the body of each container; labeling the lids is unacceptable. Site and Source Example: Site, where the specimen was removed (left foot) Source, what the specimen is (mole, lesion, CSF) Store and transport at room temperature Routine -7 Days Guthrie Clinic Lab Dependant on collection Dependant on collection APC RESISTANCE Blue.7 ml Sodium Citrate Patient Preparation: Patient must be at rest for 0 minutes prior to specimen collection Spin and separate into plastic vial. FREEZE. Transport FROZEN sample from regional office on dry ice. Hemolyzed samples will be rejected. Routine -7 Days Labcorp Lt Blue Top-Citrate Lt Blue Top-Citrate APOLIPOPROTEIN A APOLIPOPROTEIN A AND B APOLIPOPROTEIN B APTEM (ROTEM) Blue Citrate.7 ml Do Not Spin-Whole Blood Room DO NOT collect in same container with Protime/PTT Temperature APTT Blue Citrate.7 ml Correct ratio of blood to citrate is critical. Avoid hemolysis. Tube must be filled completely and mixed gently 8 times. Sample is stable 4 hrs at ROOM TEMPERATURE. Samples are kept at room temperature and centrifuged to obtain platelet poor plasma within one hour of collection. The centrifuged sample must be tested within 4 hours of collection. If testing is delayed more than 4 hours, separate the platelet poor plasma into a labeled polypropylene tube and freeze. Spin time to obtain platelet poor plasma depends on the centrifuge speed. RPM Spin Time for PPP minutes 3Lavendar 00 0 minutes 4000 Lavendar minutes minutes Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Lt Blue Top-Citrate Routine -7 Days Guthrie Clinic Lab Lt Blue Top-Citrate Lt Blue Top-Citrate Lt Blue Top-Citrate ARBOVIRUS PANEL, IGM Minimum sample: 0. ml Routine -7 Days Labcorp

12 07 GUTHRIE CLINIC TEST LIST rev. 5/08 ARGININE VASOPRESSIN REQUIREMENTS Lavender 3 ml EDTA ( tubes) Collect sample in pre-chilled EDTA tubes. Spin and separate plasma IMMEDIATELY into two ml aliquots. FREEZE. Transport on dry ice. Routine -7 Days Labcorp ARSENIC 4 HOUR URINE ARSENIC,BLOOD ARTERIAL CORD BLOOD GAS CH ARTERIAL CORD BLOOD GAS RPH ASPERGILLUS ABS ASPIRIN PF (PLATELET FUNCTION) 4 Hour Urine Acid Washed Container (Trace Element/Heavy Metal testing) PATIENT PREPARATION: Patient should refrain from eating seafood and taking herbal supplements at least 3 days prior to sample collection. Minimum sample: 5 ml urine Royal Dark Blue Lavendar ml EDTA Trace Metal PATIENT PREPARATION: Patient should refrain from eating seafood and taking herbal supplements for at least 3 days before specimen collection. Minimum sample: ml whole blood Anaerobic sample that is obtained in a heparinized blood gas syringe. Anaerobic sample that is obtained in a heparinized blood gas syringe. Store and transport sample refrigerated. Send entire container to lab. To avoid contamination, use powderfree gloves. Do not spin or separate. Whole blood required Store and Transport in a biohazard bag at room temperature and analyze within Lavendar 0 minutes of collection. Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample must be placed in a biohazard bag. Specimens are analyzed within Lavendar 0 minutes of collection. AST (SGOT) B CELL RECEPTOR IGH GENE Lavender 3 ml EDTA REARR Minimum sample: ml Routine -7 Days Labcorp Routine -7 Days Labcorp 4 HR Urine Container 4 HR Urine Container Routine -7 Days Sterile Syringe Routine -7 Days RPH RT Lab Sterile Syringe Minimum sample: 0. ml Routine -7 Days Labcorp Collection tube is a SPECIAL DRAW blue sodium citrate Keep samples at room temperature. Do Routine -7 Days Guthrie Clinic Lab Call Lab tube that is filled to the fill line. not centrifuge, separate, freeze or Call lab to supply collection tube. refrigerate. Samples must not be sent Use a gauge or larger neede. Collect blood from a to the lab through the pneumatic tube venous site. Obtain sample from an extremity free of system. peripheral venous infusions. Collect a discard tube, approximately ml. This can be any tube except EDTA. Fill the second tube to the black line. Do not under fill. Gently invert to mix. If necessary, blood may be drawn from indwelling catheters following proper blood collection protocol. Avoid contamination with EDTA. Samples are stable for 4 hours at room temperature. Routine -7 Days Guthrie Clinic Lab Do not spin or separate. Whole blood required : Store and Labcorp: Store and transport at room temperature Routine -7 Days Labcorp Royal Blue EDTA Whole Blood Royal Blue EDTA Whole Blood

13 07 GUTHRIE CLINIC TEST LIST rev. 5/08 B PERTUSSIS / PARAPERTUSSIS PCR REQUIREMENTS NOTE: If patient is insured through United Health Care please call the Laboratory Send Out Department prior to specimen collection. : Collect specimen in Blue-Top COPAN Liquid AMIES swab (NOT the blue top swab with charcoal). This is not the routine Green-Top mini-tip in Stuarts medium. ***Routine Green Mini-Tip Nasopharyngeal swabs are UNACCEPTABLE for Bordetella testing*** VCM Nasopharyngeal kits, eswabs, collection kits using Amies liquid, M4, VCM/UTM are also acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. REJECTION CRITERIA: Collection tubes with Amies gel or charcoal containing media; samples collected using cotton or calcium alginate tipped swabs; rigid shaft swabs Store and Routine -7 Days Labcorp Call Lab Amies Media No Charcoal (Blue Top) SPECIMEN for PCR. Remove excess secretions from anterior nares.. Gently pass swab through the nose and into the nasopharynx. Rotate swab on the nasopharyngeal membrane and allow the swab to remain in place 0-5 seconds to absorb organisms. 3. Remove swab and place into transport container (VCM or M4) immediately. Label samples noting a source of nasopharyngeal. BARBITURATES, URINE CONF 0 ml urine collected in a urine container with temperature strip Store and Routine -7 Days Labcorp BARIUM, SERUM Royal Dark Blue Lavendar ml No Additive SERUM Trace Spin and separate serum into a trace Routine -7 Days Labcorp Metal element container. DO NOT use a Alternate Sample: ml of plasma collected in a Royal Blue pipet. Serum must be carefully poured Trace Element EDTA tube off to avoid trace element SST-serum separator tubes are unacceptable. contamination. Store and transport refrigerated. Sample is stable up to 4 days BARTONELLA SPECIES Routine -7 Days Labcorp ANTIBODIES (IGG, IGM) WITH TITER If the antibody screen is positive, a titer is performed automatically BASIC METABOLIC PANEL Green Yellow Lithium Heparin PST BASIC METABOLIC PANEL W/REFLEX MAGNESIUM BCR-ABL GENE REARRANGEMENT, PCR Lavender 3 ml EDTA ( tubes) Minimum sample: 4 ml whole blood After collection of the sample, draw date and time, as well as sample type, must be written on the tube and included as requested information. Ship sample immediately due to the short stability of 7 hours Store and Routine -7 Days Guthrie Clinic Lab Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp Royal Blue Trace Element Royal Blue Trace Element 3

14 07 GUTHRIE CLINIC TEST LIST rev. 5/08 BENZODIAZEPINE CONF, SERUM REQUIREMENTS : 0 ml Clot Tube Labcorp: 7 ml whole blood in gray top tube : Spin and separate into plastic vial. Store and Labcorp: Do not spin or separate. Store and Routine -7 Days Labcorp Gray Top Tube BENZODIAZEPINE, URINE CONF BETA GLYCOPROTEIN AB IGA 0 ml urine collected in a urine container with temperature strip QUEST: Blue.7 ml Sodium Citrate Minimum volume ml plasma LABCORP: Mustard 5ml SST Minimum volume: ml serum Store and Routine -7 Days Labcorp QUEST: Centrifuge test tube for 5 Routine -7 Days Labcorp minutes within Lavendar 0 minutes of collection. Using a plastic pipet, remove plasma avoiding WBC/platelet buffy layer Place plasma into a plastic vial and centrifuge a SECOND time Transfer platelet-poor plasma into a new plastic vial. FREEZE immediately Store and transport frozen sample on dry ice. LABCORP: Spin and do not separate. Store and Lt Blue Top-Citrate BETA GLYCOPROTEIN AB IGG QUEST: Blue.7 ml Sodium Citrate Minimum volume ml plasma LABCORP:Gold 5ml SST Minimum volume: ml serum QUEST: Centrifuge test tube for 5 minutes within Lavendar 0 minutes of collection. Using a plastic pipet, remove plasma avoiding WBC/platelet buffy layer Place plasma into a plastic vial and centrifuge a SECOND time Transfer platelet-poor plasma into a new plastic vial. FREEZE immediately Store and transport frozen sample on dry ice. LABCORP: Spin and do not separate. Store and Routine -7 Days Labcorp Lt Blue Top-Citrate BETA GLYCOPROTEIN AB IGM QUEST: Blue.7 ml Sodium Citrate Minimum volume ml plasma LABCORP: Mustard 5ml SST Minimum volume: ml serum QUEST: Centrifuge test tube for 5 minutes within Lavendar 0 minutes of collection. Using a plastic pipet, remove plasma avoiding WBC/platelet buffy layer Place plasma into a plastic vial and centrifuge a SECOND time Transfer platelet-poor plasma into a new plastic vial. FREEZE immediately Store and transport frozen sample on dry ice. LABCORP: Spin and do not separate. Store and Routine -7 Days Labcorp Lt Blue Top-Citrate 4

15 07 GUTHRIE CLINIC TEST LIST rev. 5/08 BETA GLYCOPROTEIN ABS REQUIREMENTS QUEST: Blue.7 ml Sodium Citrate Minimum volume.5 ml plasma LABCORP: Mustard 5ml SST Minimum volume: 3ml serum QUEST: Centrifuge test tube for 5 minutes within Lavendar 0 minutes of collection. Using a plastic pipet, remove plasma avoiding WBC/platelet buffy layer Place plasma into a plastic vial and centrifuge a SECOND time Transfer platelet-poor plasma into a new plastic vial. FREEZE immediately Store and transport frozen sample on dry ice. LABCORP: Spin and do not separate. Store and Routine -7 Days Labcorp Lt Blue Top-Citrate BETA MICROGLOBULIN BETA MICROGLOBULIN, URINE BILE ACIDS, TOTAL & FRACTIONATED PATIENT PREPARATION: Overnight fast is preferred PATIENT PREPARATION: Patient should void bladder, then drink at least 500 ml of water. A urine sample should be collected within hour. PATIENT PREPARATION: Overnight fast preferred Panel includes: Total bile acids, Cholic acid, Deoxycholic acid and Chenodeoxycholic acid serum Routine -7 Days Labcorp Store and transport urine refrigerated Routine -7 Days Labcorp Spin and separate into a plastic vial. FREEZE serum. Transport frozen sample on dry ice. QUEST: accepts specimens stored at Room Temp, Refrigerated or Frozen LABCORP: requires samples to be Frozen Routine -7 Days Labcorp BILIRUBIN BODY FLUID White Fluid Tube List specimen SOURCE on the container. Store and Routine -7 Days Guthrie Clinic Lab BILIRUBIN DIRECT Routine -7 Days Guthrie Clinic Lab BILIRUBIN NEONATAL Lithium Heparin Amber SST Microtainer Protect from light. Protect from light. Spin and do not separate. Store and transport refrigerated. BILIRUBIN TOTAL Routine -7 Days Guthrie Clinic Lab Routine -7 Days Guthrie Clinic Lab White Body Fluid Tube White Body Fluid Tube Lithium Heparin Amber SST Microtainer Lithium Heparin Amber SST Microtainer Lithium Heparin Amber SST Microtainer 5

16 07 GUTHRIE CLINIC TEST LIST rev. 5/08 BK VIRUS, DNA, QUANTITATIVE REQUIREMENTS Lavender 3 ml EDTA or Minimum sample: 0.7 ml Plasma: Collect blood in sterile tubes containing EDTA as an anticoagulant, or in Plasma Preparation Tube (PPT). Collect whole blood at room temperature and separate plasma from cells within hours of collection. Transfer plasma to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen. Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST s) are recommended. Allow blood to clot at room temperature and separate serum within hour of collection. Transfer serum to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen. Routine -7 Days Labcorp Lavendar Lavendar BK VIRUS, DNA, URINE, Random urine collection QUANTITATIVE Minimum of.0 ml required BLASTOMYCES ANTIBODY, ID BLOOD CULTURE ADULT PROCEDURE: BACTERIAL AEROBIC CULTURE (C BLD) BACTERIAL ANAEROBIC CULTURE (C BLD) Store and Routine -7 Days Labcorp Routine -7 Days Labcorp Store and transport samples at room Routine -7 Days Guthrie Clinic Lab temperature. DO NOT REFRIGERATE. Blood Culture Vials Blood Culture Vials Blood Culture Vials. Disinfect blood culture bottles by rubbing stoppers with 70% alcohol and allowing it to dry.. Palpate for vein and mark with fingernail impression. 3. Using ChloraPrep One-Step Kit, pinch the wings on the applicator to break the ampule. Do not touch sponge. Wet the sponge by repeatedly pressing and releasing the sponge against the treatment area until liquid is visible on the skin. Scrub the venipuncture site for minutes with the alcohol sponge and allow to dry for minute. Discard sponge. 4. Perform venipuncture using a vacutainer safety lok butterfly needle. DO NOT repalpate the venipuncture site. Collect Amies Media No Charcoal (Blue Top) ml of blood. 5. Inoculate blood cultures bottles in the following order: aerobic, anaerobic, (if ordered) and any other blood work is collected last. See below for inoculation volumes. Lavendar. Multiple blood culture sets may be collected at the same time from separate venipuncture sites. 7. Mix bottles. 8. Label samples noting blood culture set number (i.e. set, set ), and site of collection (peripheral or indicate which line) Reference Range: No Growth Rejection Criteria: *Improper specimen collection, storage, and transport *Blood culture bottle received cracked or broken 6

17 07 GUTHRIE CLINIC REQUIREMENTS TEST LIST rev. 5/08 BLOOD CULTURE PEDIATRIC PROCEDURE: BACTERIAL AEROBIC CULTURE (C BLD) BACTERIAL ANAEROBIC CULTURE (C BLD) Store and transport samples at room temperature. DO NOT REFRIGERATE. Routine -7 Days Guthrie Clinic Lab Pediatric Blood Culture Vial Pediatric Blood Culture Vial Pediatric Blood Culture Vial. Disinfect blood culture bottles by rubbing stoppers with 70% alcohol and allowing it to dry.. Palpate for vein and mark with fingernail impression. 3. Using ChloraPrep One-Step Kit, pinch the wings on the applicator to break the ampule. Do not touch sponge. Wet the sponge by repeatedly pressing and releasing the sponge against the treatment area until liquid is visible on the skin. Scrub the venipuncture site for minutes with the alcohol sponge and allow to dry for minute. Discard sponge. 4. Perform venipuncture using a vacutainer safety lok butterfly needle. DO NOT repalpate the venipuncture site. Refer to blood culture phlebotomy protocol Lavendar for appropriate pediatric volumes to draw. 5. Inoculate blood cultures bottles in the following order: aerobic, anaerobic, (if ordered) and any other blood work is collected last. See below for inoculation volumes. Lavendar. Multiple blood culture sets may be collected at the same time from separate venipuncture sites. 7. Mix bottles. 8. Label samples noting blood culture set number (i.e. set, set ), and site of collection (peripheral or indicate which line) Reference Range: No growth Rejection Criteria: *Improper specimen collection, storage, and transport BLOOD UREA NITROGEN BODY FLUID CELL Label specimen with SOURCE. BODY FLUID CULTURE W/GS. Disinfect overlying skin with % iodine tincture.. Obtain specimen via percutaneous needle aspiration or surgery. 3. Transport specimen to lab immediately. 4. Always submit as much fluid as possible; do not submit swab dipped in fluid. Reference Range: No aerobic growth Routine -7 Days Guthrie Clinic Lab Store and Routine -7 Days Guthrie Clinic Lab <= 4 hours Room Temperature Routine -7 Days Guthrie Clinic Lab BONE MARROW DIFFERENTIAL BORDETELLA PERTUSSIS ANTIBODY, IGG BRAIN NATRIURETIC PEPTIDE BROMIDE,SERUM Rejection Criteria: Improper specimen collection, storage, and transport Store and transport room temperature Routine -7 Days Guthrie Clinic Lab Minimum sample 0.5 ml 4 ml Clot Tube SST tubes are unacceptable Spin and separate into a plastic vial. Store and Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Routine -7 Days 7

18 07 GUTHRIE CLINIC TEST LIST rev. 5/08 BRONCHIAL BRUSH CULTURE REQUIREMENTS. Place brush in ml of saline Reference Range: No aerobic growth Store and up to 4 hours. Routine -7 Days Guthrie Clinic Lab BRONCHIAL LAVAGE CULTURE W/GRAM STAIN Rejection Criteria: *Source other than bronchial brushings *Improper specimen collection, storage, and transport. BACTERIAL AEROBIC CULTURE and/or FUNGAL CULTURE: Collect specimen avoiding contamination by orapharyngeal flora.. Place bronch lavage in sputum trap or sterile container. 3. Label sample noting the sample source. Store and transport specimen refrigerated up to 4 hours. Routine -7 Days Guthrie Clinic Lab Reference range: No aerobic growth Rejection Criteria: Source other than bronchial lavage Improper specimen collection, storage, and transport BRONCHIAL WASH CULTURE W/GR. BACTERIAL AEROBIC CULTURE and/or FUNGAL CULTURE: Collect specimen avoiding contamination by orapharyngeal flora.. Place bronch washing in sputum trap or sterile container. 3. Label sample noting the sample source. Store and transport specimen refrigerated up to 4 hours. Routine -7 Days Guthrie Clinic Lab BRUCELLA (IGG, IGM) ABS WITH REFLEX BRUCELLA AB, AGGLUTINATION BUPRENORPHINE SCREEN Reference Range: Normal Respiratory Flora Rejection Criteria: Source other than bronchial washings Improper specimen collection, storage, and transport Routine -7 Days Labcorp serum tube Store and Routine -7 Days 0 ml urine collected in a urine container with temperature strip BUPROPION 4 ml Clot Tube SST tubes are unacceptable C. DIFFICILE BY PCR (STOOL). Pass unformed stool directly into a clean, dry container. Unformed stool is stool that takes the shape of it's container.. Secure cap. Label specimen noting source of stool. Patients experiencing 3 or more unformed stools within a 4 hour period should be tested for C. difficile. Reference Range: Negative Rejection Criteria: *Sources other than stool *Formed stool *>= specimens per week *Improper specimen collection, storage, and transport Store and Routine -7 Days Labcorp Spin and separate into plastic vial. Routine -7 Days Labcorp Store and transport FROZEN Store and transport sample refrigerated up to 5 days. Routine -7 Days Guthrie Clinic Lab C ESTERASE INHIBITOR PROTEIN C ESTERASE INHIBITOR, FUNCTIONAL 4 ml Clot Tube serum 4 ml Clot Tube serum Spin and separate into plastic vial Store and Spin and separate into plastic vial. FREEZE sample IMMEDIATELY. Transport frozen sample on dry ice. SST tubes are unacceptable Routine -7 Days Labcorp Routine -7 Days Labcorp 8

19 07 GUTHRIE CLINIC TEST LIST rev. 5/08 CQ COMPLEMENT COMPONENT REQUIREMENTS 4 ml Plain tube Avoid hemolysis SST tubes may be used if separated within one hour of collection Spin and separate into plastic vial. Store and C COMPLEMENT ship refrigerated C3 COMPLEMENT C4 COMPLEMENT C5 COMPLEMENT CLavendar COMPLEMENT C7 COMPLEMENT C8 COMPLEMENT : Spin and do not separate. Store and LABCORP: Spin and separate. FREEZE. : Spin and do not separate. Store and LABCORP: Spin and separate. FREEZE. : Spin and do not separate. Store and LABCORP: Spin and separate. FREEZE. CA 5 CADMIUM 4 HR URINE 4 Hour Urine Container Acid Washed Store and transport sample refrigerated. Minimum sample: 0 ml urine Transport entire container to the lab. Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Routine -7 Days 4 HR Urine Container HCL CADMIUM RANDOM URINE CADMIUM URINE (LC) 50 ml Acid Washed Container for Random Urine (for heavy metal testing) Minimum sample: 0 ml urine 4 Hour Urine Container Acid Washed Minimum sample: 0 ml urine Store and transport sample refrigerated Routine -7 Days 4 HR Urine Container HCL Store and transport sample refrigerated. Transport entire container to the lab Routine -7 Days Labcorp 4 HR Urine Container CADMIUM, BLOOD Royal Dark Blue Lavendar ml EDTA Trace Metal Minimum sample: ml Do not spin or separate. Whole blood required. Store and transport refrigerated Routine -7 Days Labcorp Royal Blue EDTA Whole Blood Royal Blue EDTA Whole Blood CALCITONIN PATIENT PREPARATION: Overnight fasting is preferred Spin and separate in to plastic vial. FREEZE sample IMMEDIATELY. Transport sample from regional office on dry ice. CALCIUM 4 HOUR URINE 4 Hour Urine HCL Container Transport entire container to lab. Store and CALCIUM RANDOM URINE Random Urine -Transfer to 0 ml urine tube for transfer. Transfer to 0ml urine tube for transport. Store and transport refrigerated CALCIUM, SERUM CALPROTECTIN, STOOL Stool in sterile container, unpreserved Store and FREEZE if samples are not tested within four days. Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab 4 HR Urine Container HCL Routine -7 Days Guthrie Clinic Lab Yellow Urine Conical Tube Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp 9

20 07 GUTHRIE CLINIC TEST LIST rev. 5/08 CALRETICULIN, MUTATION ANALYSIS CANCER ANTIGEN 5-3 CANCER ANTIGEN 9-9 CANCER ANTIGEN 7.9 CANDIDA/GARD/TRIC DNA PROBE REQUIREMENTS Lavender 3 ml EDTA or Green Sodium Heparin Collect samples Monday-Thursday ONLY. Samples should be received by reference lab within 48 hours of collection Minimum sample: 4 ml whole blood Bone marrow collected in EDTA is also acceptable PROCEDURE:. Visualize the posterior vaginal fornix. Remove excessive discharge.. Collect vaginal fluid using Collection swab. Roll the swab against the vaginal wall -3 times. Swab the lateral vaginal wall while removing the swab. 3. Immediately place the swab into specimen tube. With the swab touching the bottom of the tube, break off swab handle and cap tube. 4. Label specimen, noting source of vagina. Reference Range: Negative for Candida, Gardnerella, Trichomonas Do not spin or separate. Whole blood required. Store and transport refrigerated Store and transport sample refrigerated up to 7 hours Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab MML Swab CANNABINOIDS, CONF UR CARBAMAZEPINE AND METABOLITE Rejection Criteria: * Refrigerated specimen received greater than 7 hours * Specimen collected on swabs other than MML transport swabs * Specimen source other than vaginal 0 ml urine that is collected in a urine container with temperature strip 0 ml Clot Tube SST tubes are unacceptable CARBAMAZEPINE LEVEL 4 ml Clot Tube Spin and separate into plastic vial. Store and SST tubes are unacceptable. CARBOHYDRATE DEF TRANSFERRIN Store and Routine -7 Days Labcorp Spin and separate into plastic vial. Routine -7 Days Labcorp Store and Spin and separate into plastic vial. Serum must not contain any particles or traces of fibrin after centrifugation Store and CARBON DIOXIDE CARBOXYHEMOGLOBIN (PULM LAB) RPH and TCH: Anaerobic arterial or venous sample that is obtained in a heparinized blood gas syringe. CH :Collect in green sodium heparin tube or heparinized syringe. RPH and TCH: Sample is transported to ABG lab in a rigid container with a lid. If sent in the pneumatic tube system, the sample is placed in a biohazard bag. Samples are analyzed within Amies Media No Charcoal (Blue Top) minutes of collection. CH: Transport in a biohazard bag at room temperature and analyze within 30 minutes of collection. Routine -7 Days Guthrie Clinic Lab Routine -7 Days Labcorp Routine -7 Days Guthrie Clinic Lab STAT STAT Routine Routine RPH/TC H 30 CH 45 RPH/TC H 30 CH 45 RPH RT Lab TCH RT Lab Sterile Syringe Sterile Syringe Sterile Syringe 0

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