Alkaline Phosphatase, Bone Specific

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1 Alkaline Phosphatase, Bone Specific Order Name: ALK P BONE Test Number: REV DATE:11/5/2010 Alkaline Phosphatase, Bone Specific IMMENZ Preferred 1 ml (0.3) Serum Clot Activator SST (Red/Gray or Tiger Top) Frozen Special Allow serum samples to clot completely before centrifugation. Instructions: Testing Schedule: Tue, Thr, Sat Expected TAT: 4-6 Days Notes: The bone-specific alkaline phosphatase (BSAP) assay provides a general index of bone formation and a specific index of total osteoblast activity. BSAP and osteocalcin are the most effective markers of bone formation and are particularly useful for monitoring bone formation therapies and antiresorptive therapies. Cpt Code(s): 84080

2 Allergic Bronchopulmonary Aspergillosis (ABPA) Order Name: ASPER F AB panel Test Number: REV DATE:11/29/2010 Aspergillus fumigatus IgG Antibodies Aspergillus fumigaus Specific IgE Total Serum IgE ImmunoCAP CIA Preferred 2 ml (1) Serum Clot Activator SST (Red/Gray or Tiger Top) Room Temperature Testing Schedule: Mon - Fri Expected TAT: 3-5 Days Clinical Use: ABPA is a hypersensitivty disease of the lungs caused by Aspergillus fumigatus. It is an important complication for patients with asthma and cystic fibrosis. Cpt Code(s): 82785; 86003; 86606

3 Allergic Bronchopulmonary Aspergillosis (ABPA) Order Name: ABPA 2 panel 2 Test Number: REV DATE:11/29/2010 Aspergillus fumigatus IgG Antibodies Aspergillus fumigaus Specific IgE Total Serum IgE Aspergillus Precipitins ImmunoCAP CIA Ouchterlony Preferred 4 ml (2) Serum Clot Activator SST (Red/Gray or Tiger Top) Room Temperature Testing Schedule: Mon - Fri Expected TAT: 3-5 Days Clinical Use: ABPA is a hypersensitivty disease of the lungs caused by Aspergillus fumigatus. It is an important complication for patients with asthma and cystic fibrosis. Notes: The ABPA 2 panel is the same as the basic ABPA panel with the addition of Aspergillus Precipitins. Recent reports have emphasized the importance of both the precipitin and quantitative IgG test for Aspergillus-specific antibodies. Cpt Code(s): 86329, 86606, 86003, 82785

4 Glucagon Order Name: GLUCAGON Test Number: REV DATE:11/3/2010 Glucagon RIA Preferred 3 ml (1.1) Plasma EDTA (Lavender Top) Frozen Special Overnight fasting is required. Instructions: Stability: Room temperature: 7 Days, Refrigerated: 7 Days, Frozen: 28 Days Testing Schedule: Tues, Fri Expected TAT: 5 Days Cpt Code(s): 82943

5 Histone Antibodies Order Name: HISTONE AB Test Number: REV DATE:11/19/2010 Histone Antibodies ELISA Preferred 1 ml (0.3) Serum Clot Activator SST (Red/Gray or Tiger Top) Refrigerated Special [Pediatric minimum (0. 15mL)] Unacceptable: Plasma, Severely lipemic, bacterially contaminated, or hemolyzed Instructions: specimens. Stability after separation from cells: Ambient= 2 days, Refrigerated= 2 weeks, Frozen= 1 year (avoid repeated freeze/thaw cycles). Testing Schedule: Mon, Wed-Sat Expected TAT: 3-6 Days Clinical Use: Histone Antibody is present in 80-95% of patients with drug-induced systemic lupus erythematosus (SLE), 20-50% of patients with idiopathic SLE, and infrequently in patients with other autoimmune connective tissue diseases. Cpt Code(s): 83516

6 Immunoglobulin IgG, CSF Order Name: CSF IGG Test Number: REV DATE:11/18/2010 Immunoglobulin IgG, CSF NEPH Preferred 1mL (0.4mL) CSF (Cerebrospinal Sterile Screwtop Container Refrigerated Fluid) Special CSF must be crystalline clear. Centrifuge and separate to remove cellular material. Stability: Ambient= 8 hours, Instructions: Refrigerated= 8 days, Frozen= 1 year (if frozen within 24 hours). Testing Schedule: Sets up 3 days a week. Expected TAT: 3-5 Days Clinical Use: The concentration of CSF IgG is increased in various infections, inflammatory conditions, neoplastic diseases, and active multiple sclerosis. Cpt Code(s): 82784

7 Influenza A and B Screen Order Name: C A/B FLU Test Number: REV DATE:11/3/2010 Influenza A Screen Influenza B Screen Preferred See Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Instructions Swab Alternate See Saline nasal wash Sterile Screwtop Container Refrigerated Instructions Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Swabs in saline are only acceptable for up to 8 hours. Keep swabs refrigerated (room temperature stability is only 24hrs). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Note: Green cap minitip Swab is No Longer Acceptable. Also not acceptable are swabs in M4, M4-RT, Liquid Amies-D, Amies Clear, Modified or Liquid Stuart s and Remel M6 transport media. (the green cap minitip swab has liquid stuart s) Testing Schedule: Daily Expected TAT: 1 Day Cpt Code(s): 87804x2

8 Platelet Autoantibody Order Name: PLT AUTOAB Test Number: REV DATE:11/3/2010 Platelet Autoantibody ELISA Preferred 5 ml (3) Whole Blood EDTA (Lavender Top) Refrigerated Special Effective Nov. 3rd 2010 Instructions: All Collections Must Be Scheduled for Collection!Contact: Immunology (918) x15511 or Immunology Manager x Due to specimen integrity and stability reasons, this will ONLY be Collected at the RML Main Laboratory - Monday through Thursday..! Please make this a Separate Specimen, Keep specimen as Whole Blood and Refrigerated (2-8'C), Do Not Centrifuge! Transport specimen directly to Lab Section ASAP with Ice but Not Directly On-Ice - Do Not Freeze! Testing must begin within 24hrs. (Note: Special processing of the sample will be performed within the performing laboratory section. Hemolyzed, Icteric and Lipemic specimens will yield false results and will not be tested. ) Testing Schedule: Mon-Thr Expected TAT: 2-3 Days Clinical Use: The platelet autoantibody study is designed to detect platelet autoantibodies eluted from the patient s platelets or circulating in the patient's serum or plasma directed against GPIIb/IIIa, GPIb/IX, and GPIa/IIa. These antibodies can be detected in patients with autoimmune thrombocytopenic purpura (ITP or AITP). This test is intended to help identify patients who present with unexplained thrombocytopenia that is secondary to immune destruction. A positive test is considered diagnostic, while a negative test does not rule out the diagnosis. Repeat testing can sometimes be of benefit. Cpt Code(s): 86022

9 Respiratory Mini-screen with Reflex Order Name: VRESP3 Test Number: REV DATE:11/19/2010 Influenza A Screen Influenza B Screen Respiratory Syncytial Virus Detection Preferred 4 ml (2) Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Swab Alternate 4 ml (2) Nasal Wash Sterile Screwtop Container Refrigerated 4 ml (2) Bronchial Sterile Screwtop Container Refrigerated lavage/wash Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Keep swabs Refrigerated (2-8'C) or Frozen in UTM or other viral transport if a delay in reaching the lab is anticipated (Room Temperature is Not Recommended). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Note: Green cap minitip Swab is No Longer Acceptable. Also not acceptable are swabs in M4, M4-RT, Liquid Amies-D, Amies Clear, Modified or Liquid Stuart s and Remel M6 transport media. (the green cap minitip swab has liquid stuart s) Testing Schedule: Daily Expected TAT: 1-2 Days Clinical Use: This IEA screens for the presence of three most common respiratory viruses. Influenza A & B; and Respiratory Syncytial Virus (RSV). Notes: If negative for the quick screen, DFA will test for the presence of Adenovirus; Influenza A & B; Parainfluenza 1, 2 & 3; and Respiratory Syncytial Virus (RSV). Possible additional CPT codes: 87260, 87276, 87275, 87279x3, Cpt Code(s): 87804x2, 87807

10 Respiratory Syncytial Virus Detection Order Name: C RSV SC Test Number: REV DATE:11/19/2010 Respiratory Syncytial Virus Detection Preferred 3 ml (1) Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Swab Alternate 3 ml (1) Nasal Wash Sterile Screwtop Container Refrigerated 3 ml (1) Bronchial Sterile Screwtop Container Refrigerated lavage/wash Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Keep swabs Refrigerated (2-8'C) or Frozen in UTM or other viral transport if a delay in reaching the lab is anticipated (Room Temperature is Not Recommended). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Green cap minitip Swab is Acceptable as alternate swab type.. Testing Schedule: Daily Expected TAT: 1 Day Clinical Use: Detects presence of Respiratory Syncitial virus Cpt Code(s): 87807

11 Respiratory Syncytial Virus with Culture if Indicated Order Name: C RSV WCII Test Number: REV DATE:11/19/2010 Respiratory Syncytial Virus with Culture if Indicated Preferred 3 ml (1) Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Swab Alternate 3 ml (1) Nasal Wash Sterile Screwtop Container Refrigerated 3 ml (1) Bronchial Sterile Screwtop Container Refrigerated lavage/wash Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Keep swabs Refrigerated (2-8'C) or Frozen in UTM or other viral transport if a delay in reaching the lab is anticipated (Room Temperature is Not Recommended). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Note: Green cap minitip Swab is No Longer Acceptable. Also not acceptable are swabs in M4, M4-RT, Liquid Amies-D, Amies Clear, Modified or Liquid Stuart s and Remel M6 transport media. (the green cap minitip swab has liquid stuart s) Testing Schedule: Daily Expected TAT: 1 Day Clinical Use: Detects presence of Respiratory Syncitial virus; viral culture is performed if screen is negative. Cpt Code(s): 87807

12 Respiratory Virus Pediatric Panel 3 Order Name: VRESP3 DFA Test Number: REV DATE:11/19/2010 Influenza A Influenza B Respiratory Syncytial Virus (RSV) DFA DFA DFA Preferred 4 ml (2) Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Swab Alternate 4 ml (2) Nasal Wash Sterile Screwtop Container Refrigerated 4 ml (2) Bronchial Sterile Screwtop Container Refrigerated lavage/wash Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Keep swabs Refrigerated (2-8'C) or Frozen in UTM or other viral transport if a delay in reaching the lab is anticipated (Room Temperature is Not Recommended). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Note: Green cap minitip Swab is No Longer Acceptable. Also not acceptable are swabs in M4, M4-RT, Liquid Amies-D, Amies Clear, Modified or Liquid Stuart s and Remel M6 transport media. (the green cap minitip swab has liquid stuart s) Testing Schedule: Sun-Thr Expected TAT: 2-3 Days Clinical Use: This DFA identifies the presence of Influenza A & B and Respiratory Syncytial Virus (RSV) common for pediatric patients. Cpt Code(s): 87276; 87275; 87280

13 Respiratory Virus Screen Order Name: VRESP DFA Test Number: REV DATE:11/19/2010 Respiratory Virus Screen DFA Preferred 4 ml (2) Swab Flocked Flexible Mini-Tip Nasopharyngeal Refrigerated Swab Alternate 4 ml (2) Nasal Wash Sterile Screwtop Container Refrigerated 4 ml (2) Bronchial Sterile Screwtop Container Refrigerated lavage/wash Special The preferred specimen is Universal Transport Media (UTM) with mini-flocked Swab(Comes as a kit: RML Instructions: Supply# 50775), BD Viral Transport Media (VTM) or M5. Keep swabs Refrigerated (2-8'C) or Frozen in UTM or other viral transport if a delay in reaching the lab is anticipated (Room Temperature is Not Recommended). For Saline nasal wash: Use bulbous syringe to dispense 2 ml saline into nasal passages. Aspirate at least 1mL back into syringe and transfer to sterile container. Note: Green cap minitip Swab is No Longer Acceptable. Also not acceptable are swabs in M4, M4-RT, Liquid Amies-D, Amies Clear, Modified or Liquid Stuart s and Remel M6 transport media. (the green cap minitip swab has liquid stuart s) Testing Schedule: Sun-Thr Expected TAT: 2-3 Days Clinical Use: This DFA screens for the presence of Adenovirus; Influenza A & B; Parainfluenza 1, 2 & 3; and Respiratory Syncytial Virus (RSV). Notes: If positive additional testing will be performed for indentification at an additional charge. Possible additional CPT codes: 87260, 87276, 87275, 87279x3, Cpt Code(s): 87300

14 Vitamin B6 Order Name: VIT B6 Test Number: REV DATE:11/19/2010 Vitamin B6 HPLC Preferred 1 ml (0.5) Plasma Lithium Heparin PST (Light Green Top) Frozen Alternate 1 ml (0.5) Plasma Sodium Heparin (Green Top) Frozen 1 ml (0.5) Serum Clot Activator (Red Top, No-Gel) Frozen Special Note: EDTA Plasma is No Longer Acceptable specimen type. (Specimens collected in EDTA yield a higher Instructions: concentration of the biologically active form of vitamin B6, therefore EDTA is not acceptable. ) Collect the specimen after an overnight fast. Collect in a Light Protected Sodium or Lithium Heparin plasma separator tube. Serum (plain red) is also acceptable. Please separate Plasma from cells and Freeze 1mL(0. 5) of plasma in light protected plastic aliquot tube ASAP. Make sure that patient information is both above and below light protection. CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Protect from light during collection, storage, and shipment. Unacceptable Specimens: EDTA Plasma, Non-frozen specimens, Not Light Protected, Improperly Labels Specimens. Stability: After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 2 months. Testing Schedule: Sun-Sat Expected TAT: 2-4 Days Clinical Use: Vitamin B6 is a cofactor in many metabolic pathways including heme synthesis. Vitamin B6 deficiency may be observed in patients with metabolic disorders, secondary to therapeutic drug use, or alcoholism. Deficiency affects the function of the immune system. Cpt Code(s): 84207

Acid Fast Bacilli (AFB) Culture and Smear

Acid Fast Bacilli (AFB) Culture and Smear Acid Fast Bacilli (AFB) and Smear Order Name: C AFB Test Number: 6000100 Acid Fast Bacilli (AFB) and Smear Preferred 5 ml (3) Sputum Sterile Screwtop Container Room Temperature Alternate 1 5 ml (3) Tissue