(134.5 mmol/l NaCl+20 mmol/l NaHCO mmol/l KCl+1.3 mmol/lcacl mmol/l MgCl 2 ph8.1)

Transcription

1 1,2, 1,2, 1,2,3, 1,2, 1, 1,2, 1,2, 1, 1, E ) 196 Siganus canaliculatus ATP CK SDH LDH SOD CAT GR GSH-PX GR P< U/L±17.14 U/L U/L±22.24U/L7 P<0.05 Siganus canaliculatus S917 A Siganus canaliculatus [1,2] [3~5] [3] [6~11] [12] LHRH-A 2 (PT) HCG 90% (2006BAD03B08-08) (2006DKA30470_004) (2007T02) (1978-) : , hxr828@ 126.com longzhen2885@hotmail.com 16

2 D (134.5 mmol/l NaCl+20 mmol/l NaHCO mmol/l KCl+1.3 mmol/lcacl mmol/l MgCl 2 ph8.1) 12% 1:1 0.2 ml 1 cm 1 min % 1.2 CK ATP SDH LDH SOD CAT GR GSH-PX CK ATP ATP ATP 1 µmol ATP USDH ULDH ml min 1 µmol 1 USOD SOD 50% SOD SOD U CAT 1 µmol 1 (U) GR NADPH 1 mmol/l U GSH-PX 0.1 ml 37 5 min GSH 1µ mol/l Excel Statistica Version 6.0 One-Way ANOVA P< CK ATP 1 2 CK ATP 1 CK Fig.1 Effects of cryopreservation on the CK 2 Fig.2 ATP Effects of cryopreservation on the total ATP U/mL±0.13 U/mL U/mL±0.56 U/mL P< ATP 9.20 U/mL±0.28 U/mL 4.18 U/mL±0.81 U/mL ATP P< SDH LDH 3 4 SDH LDH Marine Sciences/Vol.33,No.7/

3 3 SDH Fig.3 Effects of cryopreservation on SDH 4 LDH Fig.4 Effects of cryopreservation on LDH 3 SDH U/mL±2.52 U/mL 9.33 U/mL±1.53 U/mL SDH P < LDH U/L± U/L U/L± U/L LDH P < SOD CAT 5 6 SOD CAT 5 SOD Fig.5 Effects of cryopreservation on SOD 6 CAT Fig.6 Effects of cryopreservation on CAT activity 5 SOD U/mL±12.66 U/mL SOD U/mL±50.55 U/mL SOD P < CAT U/mL±15.04 U/mL CAT U/mL±12.70 U/mL CAT P < GSH-PX GR 7 8 GSH-PX GR 18

4 7 GSH-PX 8 GR Fig.7 Effects of cryopreservation on GSH-PX Fig.8 Effects of cryopreservation on GR activity 7 GSH-PX U/(min ml)±2.11 U/(min ml) GSH-PX U/(min ml)±1.78 U/(min ml) GSH-PX P < GR U/mL±17.14 U/mL GR U/mL±22.24 U/mL GR P < SDH SDH H + ATP SDH SDH [13~15] ATP ATP ADP ATP ATP ATP Bilgeri [16] ATP ATP [17] ATP SDH [18] SDH ATP SDH ATP [19] SDH Babiak [20] Oncorhynchus mykiss CK ATP LDH CK LDH CK ATP [21,22] LDH LDH [23~25] [26] Bos mutus LDH [27] LDH LDH LDH 3.2 Marine Sciences/Vol.33,No.7/

5 DNA [28] [29] SOD CAT Sreejith [30] SOD Marta [31] 5 SOD CAT SOD CAT GSH-PX (GSH) GSH-PX GSH-PX (Microtus agrestis) 22 8 GSH-PX 43% (ROS) ROS [32] GR (GSSG) (GSH) GSH (-SH) GR GR GR GR GSH [33] GSH-PX GR [34] Chlamydomonas sp. GR GR 3.3 GR 4 3 GSH/GSSG [1],,,. [J].,1999,14(4): [2],,,. [J]., 2008, 32(1):77-82 [3],,,. [J].,1997,2(4): [4]. [J].,1987,9(3):

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7 [32] Colin S, Jane S, Laren M, et al. Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal[j]. Free Radical Biology & Medicine, 2000, 28: [33] Leipner J, Fracheboud Y, Stamp P, et al. Effect of growing season on the photosynthetic apparatus and leaf antioxidative defenses in two maize genotypes of different chilling tolerance [J]. Environ Exp Bio,1999, 42: [34],,,. ICE-L [J].,2006, 37(2): Effects of cryopreservation on enzyme activity of Siganus canaliculatus spermatozoa HUANG Xiao-rong 1,2, ZHANG Long-zhen 1,2, ZHUANG Ping 1,2,3, JIANG Qi 1,2, LIU Jian-yi 1,YAO Zhi-feng 1,2, ZHAO Feng 1,2, ZHANG Tao 1, FENG Guang-peng 1,2 (1.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai , China; 2.College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai , China; 3.Aquaculture Division, E-Institute of Shanghai University, Shanghai , China) Received:Oct., 16, 2008 Key words: cryopreservation; Siganus canaliculatus; spermatozoa; enzyme activity Abstract: This paper studied the effects of cryopreservation(-196 ) on the enzyme activity(total ATP, CK, SDH, LDH, SOD, CAT, GSH-PX and GR) in Siganus canaliculatus spermatozoa. The changes of enzyme activity were analysed in the spermatozoa using the reagent box respectively. The results showed the activities of spermatozoa dropped after cryopreservation, GR activities increased significantly(p<0.05), the activities added from U/L±17.14 U/L to U/L±22.24 U/L, other seven kinds of enzyme activities descended significantly(p<0.05). The activities of total ATP, CK, SDH dropped from 9.20 U/mL±0.28 U/mL,25.59 U/mL±0.13 U/mL and U/mL±2.52 U/mL to 4.18 U/mL±0.81 U/mL,17.87 U/mL±0.56 U/mL and 9.33 U/mL±1.53 U/mL respectively, the activities of LDH, SOD and CAT declined from U/L± U/L, U/mL±12.66 U/mL and U/mL±15.04 U/mL to U/L± U/L, U/ml±50.55 U/ml and U/mL±12.70 U/mL respectively. Activity of GSH-PX dropped from U/(min ml)±2.11 U/(min ml) to U/(min ml)±1.78 U/(min ml).cryopreservation had a great effect on the enzyme activity and spermatozoa energy of Siganus canaliculatus. ( : ) 22