Progress of Lens Biochemistry Research

Transcription

1 Progress of Lens Biochemistry Research

2 Documenta Ophthalmologica Proceedings Series volume 8 Editor H. E. Henkes Dr. W. Junk bv Publishers The Hague 1976

3 Progress of Lens Biochemistry Research Volume in honour of Prof. Dr. med. J. Nordmann edited by O. Hockwin Dr. W. Junk bv Publishers The Hague 1976

4 Dr W. Junk b.v. Publishers 1976 No part of this book may be reproduced and/or published in any form, by print, photoprint, microfilm or any other means without written permission from the publishers. ISBN-13: : / e-isbn-13:

5 CONTENTS O. HOCKWIN (Bonn): Preface VII A. BRINI, M~E. STOECKEL, A. POR TE & J. KLETHI (Strasbourg): Intra-Nuclear inclusions in the lens fibres observed in the galactose-induced cataract in the young rat T. KUW ABARA & S.OKISAKAA (Bethesda, Boston): Electron microscopic study of cataractous lenses of diabetic sand rats (Psammomys obesus) D.E.S. TRUMAN, RUTH M. CLAYTON, A.G. GILLIES & H.I. MACKENZIE (Edinburgh): RNA synthesis in the lenses of normal chicks and in two strains of chicks with hyperplasia of the lens epithelium RUTH M. CLA YTON, D.E.S. TRUMAN, J. HUNTER, P.G. ODEI GAH & D.1. DE POMERAI (Edinburgh): Protein synthesis and its regulation in the lenses of normal chicks and in two strains of chicks with hyperplasia of the lens epithelium J. FRANCOIS & V. VICTORIA-TRONCOSO (Ghent): Epithelium of the adult lens in tissue culture M. IWIG & D. GLASSER (Halle): On the stability of the differentiated state - Reactivation of lens fiber cells to mitotic growth 47 R.C. BRIGGS, N. WAINWRIGHT & H. ROTHSTEIN (Burlington): On the chromosomal proteins of lens epithelium 57 J.R. REDDAN, N.J. UNAKAR, EV AMARIE KRASICKY & DO- ROTHY WILSON (Rochester): Triggering of mitosis in the cultured mammalian lens by proteolytic enzymes J. BOURS, KARIN DOEPFMER & O. HOCKWIN (Bonn): Isoelectric focusing of crystallins from different parts of the bovine and dog lens in dependence on age H. BLOEMENDAL & ANNEKE ZWEERS (Nijmegen): Improved separation of LM (Low molecular weight) crystallins H.J. HOENDERS (Nijmegen): Changes in the subunit structure of a-crystallin from bovine and rabbit eye lenses 105 K. SATOH & M. BANDO (Chiba/Tokyo): A comparative study of rabbit lens proteins H. MAISEL, J. ALCALA & N. LIESKA (Detroit): The protein structure of chick lens fiber cell membranes and intracellular matrix A.A. SWANSON, U. HAHN & O. HOCKWIN (Charleston/Bonn): Investigation of proteolytic activity in bovine lenses with age I. The exopeptidases W.B. RA THB UN (Minneapolis): Lens glutathione: Metabolism and possible functions V.N. REDDY & R.F. HAN (Rochester): Protein-bound glutathione in mammalian lenses and in galactose cataract H.N. FUKUI, L.O. MEROLA & J.H. KINOSHITA (Bethesda): The effect of oxidants on the membrane sulfhydryl groups of the lens V

6 K.l. DILLEY & RUTH VAN HEYNINGEN (Oxford): Some aspects of human lens metabolism: Glycolysis and protein synthesis C. OHRLOFF, F. BOUS & O. HOCKWIN (Bonn): Competition for glucose-6-phosphate by enzymes of bovine lenses H.L. KERN, S.A. OSTROVE & C.-K. HO (Bronx): Effect of aging on transport of amino acids by bovine lens Y. OBARA, E. COTLlER, R. LINDBERG & 1. HORN (Chicago): Cholesterol, cholesterol ester, and sphingomyelin complexed to protein of normal human lens and senile cataracts. 193 INGE KORTF, O. HOCKWIN, F. WINKLER & P. RABE (Bonn): Studies on the mode of action of I-hydroxy-pyrido-(3,2a)-5- phenoxazone-3-carboxylic acid in bovine lenses KLETHI (Strasbourg): Sodium and potassium in the normal human lens in relation with age J.W. PA TTER SON (Farmington): Cell volume regulation in the lens G. DUNCAN & RUTH V AN HEYNINGEN (Oxford): Differences in the calcium binding capacity of normal and cataractous lenses J.L. RAE & T.R. ST ACEY (Galveston): Intracellular markers in the crystalline lens of the rat S. LERMAN (Atlanta): Lens fluorescence in aging and cataract formation J.F.R. KUCK jr. (Atlanta): Effect of long-wave ultraviolet light on the lens. II. Metabolic inhibitors synergistic with UV in vitro S. ZIGMAN (Rochester): Tryptophan excited states in the lens 267 W. MANSKI, A.T. YORK, G.R. MERRIAM Jr., B.V. WORGUL & A. SZECHTER (New York): On the independent action of cataractogenic agents x-rays and galactose 275 H.-R. KOCH, KATRIN DOLDI & O. HOCKWIN (Bonn): Naphthalene cataracts in rats. Association of eye pigmentation and cataract development S.D. VARMA & J.H. KINOSHITA (Bethesda): Topical treatment of galactose cataracts V.E. KINSEY (Rochester): Studies on the Crystalline lens. XXIV. Bicarbonate content and flux determinations VI

7 PREFACE On August 25,1976 Prof. Dr. med. JEAN NORDMANN, Emeritus Professor of Strasbourg University, will celebrate his 80th anniversary. The task of paying tribute to Professor NORDMANN's many services to ophthalmology in a way worthy of the occasion is so difficult that we decided to concentrate on the focal point of his life's work. In this issue scientists engaged in lens research from allover the world offer reports of their latest findings together with their best wishes and felicitations as a birthday gift to the Nestor of lens research. They thereby express their gratitude to Professor NORDMANN, who, with the lifelong indefatigable enthusiasm of a scientist, has most successfully persued the problems of lens metabolism. It is our sincerest hope that we may look forward to many more years in which to take advantage of Professor NORDMANN's superior knowledge. Among the contributions to this festive donation we miss the works of some authors who have always held Professor NORDMANN 's special interest. The editor has received several letters of longstanding friends who would have liked to submit a manuscript but had been unable to meet the deadline: Professor NORDMANN may rest assured that they, too, belong to the great number of congratulators from our field of research. We have been able to make this dedication thanks to Professor HENKES who readily agreed to publish our contributions in Documenta Ophthalmologica. Dr. Junk publishers have done their utmost to ensure prompt publication. OTTO HOCKWlN, Bonn VII