Sarah Michelle Totten Curriculum Vitae

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1 Sarah Michelle Totten Curriculum Vitae Stanford University School of Medicine 3155 Porter Drive, MC5483 Palo Alto, CA Office: Cell: EDUCATION University of California, Davis Ph.D. Analytical Chemistry, March University of California, Riverside Bachelor of Science, Chemistry, March Riverside, CA RESEARCH EXPERIENCE Postdoctoral Researcher (Current Position) Canary Center for Cancer Early Detection Stanford University School of Medicine, Department of Radiology March 2014 Present Advisor: Assistant Professor Sharon J. Pitteri Palo Alto, CA Currently developing chromatography and tandem mass spectrometry analytical techniques for the glycoproteomic analysis and site-specific glycoform mapping of blood and tissue-derived human glycoproteins. These methods will be incorporated into a proteomic workflow for the identification, quantitation, and validation of biomarkers for early detection of cancers, with a focus on prostate and pancreatic cancers. Graduate Student Researcher University of California, Davis, Department of Chemistry September 2009 March 2014 Advisor: Professor Carlito B. Lebrilla Thesis: Mass Spectrometric Analysis of Human Milk Oligosaccharides: Correlating Glycan Expression to Mother and Infant Health Developed high-throughput analytical methods for profiling free glycans native in human milk and infant feces using LC-MS/MS for large data sets. These methods were used to determine variation in glycan type and abundance with respect to maternal disease state and the anthropometry, morbidity, and gut microbiota of the infant. Totten Page 1

2 HONORS & AWARDS Best Poster Award, Canary Symposium for Cancer Early Detection, Stanford, CA (May 2017). US Human Proteome Organization (HUPO) Travel Stipend, 13 th Annual Conference, San Diego, CA (March 2017). American Association for Cancer Research (AACR) Minority Scholar in Cancer Research Award (January 2017). Stanford University School of Medicine, Hispanic Center of Excellence (HCOE) Health Services and Resources Administration, Bureau of Health Professions, Postdoctoral Fellowship (December 2015 June 2016). American Society for Mass Spectrometry (ASMS) Student Travel Stipend, Asilomar Conference (October 2014). Alfred P. Sloan Minority PhD Scholar ( ). PUBLICATIONS Totten SM, Feasley C, Bermudez A, Pitteri SJ. Parallel comparison of glycopeptide enrichment techniques reveals extensive glycoproteomic analysis of plasma enabled by SAX-ERLIC. J Proteome Res. 2017; 16 (3): Totten SM, Kullolli M, Pitteri SJ. Multi-lectin affinity chromatography for separation, identification, and quantitation of intact protein glycoforms in complex biological mixtures. Methods in Mol Biol. 2017; 1550: Davis JCC, Totten SM, Huang JO, Nagshbandi S, Kirmiz N, Garrido DA, Lewis ZT, Wu LD, Smilowitz JT, German JB, Mills DA, Lebrilla CB. Identification of oligosaccharides in feces of Breast-fed Infants and their correlation with the gut microbial community. Mol. Cell. Proteomics, 2016;15(9): Garrido DA, Ruiz-Moyano S, Kirmiz N, Davis JCC, Totten SM, Lemay DG, Ugalde JA, German JB, Lebrilla CB, Mills DA. A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. Longum SC596. Sci. Rep., 2016; 6: Charbonneau MR, O Donnell D, Blanton LV, Totten SM, Davis JCC, Barratt MJ, Cheng J, Guruge J, Talcott M, Bain JR, Muehlbauer MJ, Ilkayeva O, Wu C, Struckmeyer T, Barile D, Mangani C, Jorgensen J, Fan Y, Maleta K, Dewey KG, Ashorn P, Newgard CB, Lebrilla CB, Mills DA, Gordon JI. Sialylated Milk Oligosaccharides Promote Microbiota-Dependent Growth in Models of Infant Undernutrition. Cell 164 (5): doi: /j.cell Totten Page 2

3 Lewis ZT, Totten SM, Smilowitz JT, Popovic M, Parker E, Lemay DG, Van Tassell ML, Miller MJ, Jin YS, German JB, Lebrilla CB, Mills DA. Maternal Fucosyltransferase 2 status affects the gut bifidobacterial communities of breastfed infants. Microbiome, 2015:3(13). Totten SM, Wu DL, Parker EA, Davis JC, Hua S, Stroble C, Ruhaak LR, Smilowitz JT, German JB, Lebrilla CB. Rapid-throughput glycomics applied to human milk oligosaccharide profiling for large human studies. Anal Bioanal Chem, 2014;406(30): Huang Q, Ruhaak LR, Totten SM, Smilowitz JT, German JB, Lebrilla CB. Label-free absolute quantitation of oligosaccharides using multiple reaction monitoring. Anal Chem, 2014;86(5): Hua S, Hu CY, Kim BJ, Totten SM, Oh MJ, Yun N, Nwosu CC, Yoo JS, Lebrilla CB, An HJ. Glyco-Analytical Multispecific Proteolysis (Glyco-AMP): A Simple Method for Detailed and Quantitative Glycoproteomic Characterization. J Proteome Res.2013;12(10): Smilowitz JT, Totten SM, Huang J, Grapov D, Durham HA, Lammi-Keefe CJ, Lebrilla C, German JB. Human Milk Secretory Immunoglobulin A and Lactoferrin N-Glycans Are Altered in Women with Gestational Diabetes Mellitus. J. Nutr. 2013;143(12): Ruiz-Moyano S, Totten SM, Garrido DA, Smilowitz JT, German JB, Lebrilla CB, et al. Variation in Consumption of Human Milk Oligosaccharides by Infant Gut-Associated Strains of Bifidobacterium breve. Appl Environ Microbiol. 2013;79(19): Totten SM, Zivkovic AM, Wu S, Ngyuen U, Freeman SL, Ruhaak LR, et al. Comprehensive profiles of human milk oligosaccharides yield highly sensitive and specific markers for determining secretor status in lactating mothers. J Proteome Res. 2012;11(12): Stanley F, Warner A, Gutierrez SM, Stalcup A. Heparin-induced circular dichroism of chloroquine. Biochemical and biophysical research communications. 2009;388(1): Eldridge SL, Korir AK, Gutierrez SM, Campos F, Limtiaco JF, Larive CK. Heterogeneity of depolymerized heparin SEC fractions: to pool or not to pool? Carbohydrate Research. 2008;343(17): Korir AK, Limtiaco JF, Gutierrez SM, Larive CK. Ultraperformance ion-pair liquid chromatography coupled to electrospray time-of-flight mass spectrometry for compositional profiling and quantification of heparin and heparan sulfate. Analytical Chemistry. 2008;80(4): Totten Page 3

4 CONFERENCE PRENTATIONS This list describes oral presentations and selected poster presentations at national conferences from 2010 to present Global and site-specific glycosylation analysis of prostate cancer tissue-derived glycoproteins using HCD Product-Dependent ETD Mass Spectrometry and Lectin Affinity Chromatography. S.M. Totten, C. Tanimoto, A. Bermudez, A. Hembree, J.D. Brooks, S.J. Pitteri. American Society for Mass Spectrometry 65th Annual Conference in Indianapolis, Indiana. (Poster, June 8, 2017). Aberrant Glycoprotein Expression in Recurrent and Non-Recurrent Prostate Cancer Tissue. S.M. Totten, C. Tanimoto, A. Bermudez, A. Hembree, J.D. Brooks, S.J. Pitteri. American Association for Cancer Research (AACR) Annual Meeting in Washington D.C. (Poster, April 3, 2017). Quantitative Glycoproteomic Profiling of Prostate Cancer Tissue Reveals Alterations in Glycosylation Patterns in Men with Recurrent Disease. S.M. Totten, C. Tanimoto, A. Bermudez, A. Hembree, J.D. Brooks, S.J. Pitteri. US Human Proteome Organization (HUPO) Annual Conference in San Diego, CA. (Poster, March 20, 2017). An Integrated Workflow for Enrichment, Separation, and Quantitation of Plasma Glycoproteins in Prostate Cancer and Benign Prostate Hyperplasia. S.M. Totten, M. Kulloli, C. Tanimoto, J. Brooks, S.J. Pitteri. American Society for Mass Spectrometry 63 rd Annual Conference in St. Louis, Missouri. (Oral Presentation, June 1, 2015). In-depth Quantitative Analysis of Protein Glycoforms in Human Prostate Cancer Plasma. S.M. Totten, M. Kullolli, J.D. Brooks, S.J. Pitteri. American Association for Cancer Research (AACR) Annual Meeting 2015 in Philadelphia, Pennsylvania, USA. (Poster, April 20, 2015). In-Depth Quantitative Glycoproteomic Profiling of Human Plasma using Multi-Lectin Affinity Chromatography and Mass Spectrometry. S.M. Totten, M. Kullolli, J.D. Brooks, S.J. Pitteri. American Society for Mass Spectrometry 2014 Annual Asilomar Conference in Pacific Grove, California. (Poster, October 10, 2014). Mass Spectrometry Based Glycan Arrays for Determining Specificity of Glycosidases in Bacteria. S.M. Totten, S. Ruiz-Moyano, D. Mills, C.B. Lebrilla. American Society for Mass Spectrometry 61 st Annual Conference in Minneapolis, Minnesota, USA. (Poster, June 12, 2013). High-Throughput Glycomics Profiling of Human Milk. S.M. Totten, L. Dimapasoc, L.R. Ruhaak, C.D. Stroble, J.T. Smilowitz, C.B. Lebrilla. Ninth Annual National Carbohydrate Symposium, Alberta Glycomics Centre in Banff, AB, Canada. (Oral presentation, May 10, 2013). A Rapid Screening Method for Oligosaccharide Function in Bacterial Consumption by nano-lc Chip/Time-of-Flight Mass Spectrometry. S.M. Gutierrez, S. Ruiz-Moyano, S. Wu, D. Mills, C.B. Lebrilla. American Society for Mass Spectrometry 60 th Annual Conference in Vancouver, BC, Canada. (Oral Presentation, May 24, 2012). Totten Page 4

5 Rapid Determination of Human Milk Oligosaccharide Variation Between Lewis Blood Type and Seasonal Conditions. S.M. Totten, S. Wu, N. Tao, A. Zivkovic, A.M. Prentice, J.B. German, C.B. Lebrilla. American Society for Mass Spectrometry 59 th Annual conference in Denver, Colorado, USA. (Poster, June 6, 2011). TEACHING EXPERIENCE & MENTORSHIP Stanford University Palo Alto, CA Guest Lecturer, Glycosylation Analysis by Mass Spectrometry, Mass Spectrometry and Proteomics BIOS 227, Stanford University. (June 16, 2017). Association for Women in Science (AWIS) Palo Alto Chapter, Have served as a mentor to an early-career graduate students in STEM field at Stanford University. ( academic year) Academic Mentorship Have closely mentored two undergraduate students at Stanford who have successfully transitioned to graduate degree programs in STEM. University of California at Davis Guest Lecturer, Orbitrap Mass Spectrometry Theory, Graduate-Level Mass Spectrometry, Department of Chemistry, UC Davis (February 27, 2017). Guest Lecturer, Graduate Seminar in Food Science and Technology, Department of Food Science and Technology. (February 27, 2013). General Chemistry, Teaching Assistant (September 2009 June 2010). Instrumental Analysis, Teaching Assistant (September 2010 March 2011). Academic Mentorship Have closely mentored two chemistry graduate students at UC Davis who have exceled as independent scientists in industry positions. Totten Page 5

6 ADDITIONAL TRAINING Stanford Cancer Institute, Stanford University School of Medicine Comprehensive Cancer Research Training Program (September 15-18, 2014). Palo Alto, CA American Society for Mass Spectrometry Short Courses Mass Spectrometry of Peptides and Proteins (June 2013). MS/MS: An Introduction (June 2011). Minneapolis, MN Denver, CO University of California, Davis Biotechnology Short Courses Multivariate Data Analysis (August 2012). Proteomics Course (July 2012). PROFESSIONAL AFFILIATIONS American Society for Mass Spectrometry (2011 present). American Association for Cancer Research (2015 present). AACR - Minorities in Cancer Research (2016-present). American Society for Biochemistry and Molecular Biology (2016 present). US Human Proteome Organization (2017). Totten Page 6

7 REFERENCES Assistant Professor Sharon J. Pitteri Department of Radiology Stanford University School of Medicine Canary Center at Stanford for Cancer Early Detection 3155 Porter Drive, MC5483 Palo Alto, CA Office: (650) Professor Carlito B. Lebrilla Department of Chemistry University of California, Davis 1 Shields Avenue Office: (530) cblebrilla@ucdavis.edu Professor James D. Brooks Keith and Jan Hurlbut Professor in the Stanford University School of Medicine Professor of Urology 300 Pasteur Drive, Room S-287 Stanford, California Office: (650) jdbrooks@stanford.edu Additional References Available Upon Request Totten Page 7