Bordetella pertussis. CFDN B. pertussis. McFarland No. 1 1/ Key words: Bordetella pertussis, American Society for Microbiology

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1 Bordetella pertussis / BD EZ(BD), 1P1504 B. pertussis ATCC9797 McFarland No. 1 1/2 CFDN B. pertussis ATCC ml () Key words: Bordetella pertussis, 2008 PCR PCR ( ) 171 TEL: FAX: E-male: j-okada@mail.falco.co.jp American Society for Microbiology (ASM) 1) Bordetella pertussis 24) B.pertussis 5, 6) ASM 1) B. pertussis 17

2 / BD, EZ BD, 1P1504 B. pertussis CFDN Lot. No. VP8E021H 1) Thompson 7) B. pertussis ATCC9797 McFarland No. 1 1/ ml 10 n 25 ml CFDN ml B. pertussis ATCC McFarland 100 1ml 100 Table 1. Redution of inoculums sizes in five commercial swabs for Bordetella pertussis transport. Swab (transport media) Company Storage time (h) Seed swab gi Eiken (Cary-Blair) Seed swab giii Eiken (Amies with charcoal) BD BBL CultureSwab Plus Single (Amies) BD BBL CultureSwab EZ (no media) Men-tip (no media) Colony count (log 10 CFU/ml) a) Eiken Recovery () from inoculum b) Count () relative to 0 h c) Colony count (log 10 CFU/ml) a) Eiken Recovery () from inoculum b) Count () relative to 0 h c) Colony count (log 10 CFU/ml) a) BD Recovery () from inoculum b) Count () relative to 0 h c) Colony count (log 10 CFU/ml) a) BD Recovery () from inoculum b) Count () relative to 0 h c) Colony count (log 10 CFU/ml) a) d) JCB Recovery () from inoculum b) Count () relative to 0 h c) Eiken, Eiken Chemical Co., Ltd.; BD, Nippon Becton Dickinson Company, Ltd.; JCB, JCB Industry Ltd. a) Colony count: The base 10 logarithm of Bordetella pertussis ATCC9797 inocula per milliliter loaded in each swab. b) Recovery () from inoculum: Recovery rate of inocula from swabs when the initial inoculum was absorbed into each swab. c) Count () relative to 0: Relative reduction rate of inocula when the size of Bordetella pertussis ATCC 9797 inocula loaded into each swab at 0 h was defined as 100. d) The hyphen is less than sensitivity. 18

3 131 2) McFarland No. 1 1/2 B. pertussis ATCC9797 (25) (5) 3) 1ml NEFA-V2 -CoA Fig. 2. Comparison of inoculums of Bordetella pertussis ATCC9797 in each storage method of ambient temperature (25) and refrigerator (5) 1. Table 1 5 Fig EZ EZ 70.3, , , Fig. 1. Reduction of inoculums of Bordetella pertussis ATCC9797 in each commercial transport swabs When we defined with 100 inoculums which loaded Bordetella pertussis ATCC 9797 in a swab, count () relative to 0 h was calculated Fig. 3. Comparison of concentration of fatty acid in each commercial transporting swab Fig Fig. 3 EZ 60 mmol/l 8) mmol/l EZ 17 mmol/l

4 132 ASM 1) B. pertussis (nasopharyngeal specimen) B. pertussis B. pertussis EZ Hallander 9) 11 EZ, B. pertussis B. pertussis 5, 6) B. pertussis ASM 1 24 EZ, 1 3 B. pertussis B. pertussis ASM 1) 1 EZ 1) MacGowan, K. L Bordetella cultures. p , In: Clinical Mirobiology Procedures Handbook, 2nd ed. (H. D. Isenberg, ed.), American Society for Microbiology, Washington, D.C. 2) BD BBL Culture Swab Plus TM 27: ) Appelbaum, P. C., S. K. Spangler, R. Crist, et al Survival of bacteria in Difco CultureSwab and Marion Culturette II transport 20

5 133 systems. J. Clin. Microbiol. 26: ) Horn, K. V., C. Toth, J. Wegienek Viability of aerobic microorganisms in four swab systems. ASM 98th general meeting. C ) Morrill, W. E., J. M. Barbaree, B. S. Fields, et al E#ects of transport temperature and medium on recovery of Bordetella pertussis from nasopharyngeal swabs. J. Clin. Microbiol. 26: ) Hoppe, J. E., A. Weib Recovery of Bordetella pertussis from four kinds of swabs. Eur. J. Clin. Microbiol. 6: ) Thompson, J. S., D. E. Smith Comparison of rayon and dacron swabs in amies medium for Bordetella pertussis transport. ASM 99th general meeting. C-31. 8) (FFA) p , ) Hallander, H. O., E. Reizenstein, B. Renemar, et al Comparison of nasopharyngeal aspirates with swabs for culture of Bordetella pertussis. J. Clin. Microbiol. 31: Reduction of Inoculums in Commercial Transport Swabs for Bordetella pertussis Transport Junpei Okada, Masaru Komatsu, Hiroto Tanaka, Kiyoko Katoh, Kimika Murakami, Emu Fujiwara, Koji Matsuoka, Takuya Maede Central Laboratory Technical Section 3, FALCO Biosystems Ltd., 171 Tai-nishiarami Kumiyama-cho, Kuze-gun, Kyoto , Japan We investigated reduction of inoculums of Bordetella pertussis in each commercial transport swabs. Transport media were chosen as following; (1) Seed swab gi (Eiken Chemical, Ltd., Cary-Blair media), (2) Seed swab giii ( Eiken Chemical, Ltd., Amies with charcoal), (3) BD BBL Culture Swab Plus Single (BD, Amies), (4) BD BBL Culture Swab EZ (BD, additive-free of media) and (5) Mentip (JCB Industry Ltd., the swab was kept in a sterilized spitz). There was inoculums size from 3.2 to 13.5 of Bordetella pertussis ATCC9797 in each commercial transport swabs. Reduce of inoculums size was from 0 to 70.3 for 24 hours of the room temperature storage. (3) and (5) in particular remarkable reduced inoculums size. We demonstrated that all of five commercial swabs were hard to take inoculums, inoculums sizes were remarkably decrease caused by storage, and it might be di$cult to perform Bordetella pertussis culture by the swab method. 21