THE PROBIOTIC PRODUCT RANGE HAS SET A NEW INDUSTRY STANDARD BY OFFERING THREE PRODUCTS WITH A PROVEN POTENCY MULTI STRAIN BACTERIA AT THE TIME OF
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1 THE PROBIOTIC PRODUCT RANGE HAS SET A NEW INDUSTRY STANDARD BY OFFERING THREE PRODUCTS WITH A PROVEN POTENCY MULTI STRAIN BACTERIA AT THE TIME OF CONSUMPTION WHICH ARE STABLE AT NORMAL ROOM TEMPERATURE SO NO NEED FOR INCONVENIENT REFRIGERATION.
2 THE IMPORTANCE OF A TRIED AND TESTED PROBIOTIC SUPPLEMENT When choosing a probiotic supplement, the viability and potency must be considered to ensure the supplement is efficacious and value for money. Bacteria that are to be used for bowel flora supplementation require certain clearly definable properties in order to be effective: They must belong to a strain that can survive the passage through the stomach acid - a hazard that kills most bacteria. They should be able to grow in the presence of bile, which is another demanding requirement. Upon reaching the lower intestine they must be able to implant there and multiply as an on-going population. The ability to implant is related to the strain s ability to adhere to the wall of the intestines. In addition the supplement formulation needs to be sufficiently stable so it can be conveniently stored and transported without significant loss of activity (i.e. retain a guaranteed number of viable organisms). THE NUTRIGOLD PRO-OTIC RANGE FACT: On average, each Pro-otic capsule contains 3.5 billion viable organisms whilst Mega Strength Pro-otic offers 25 billion viable organisms AT TIME OF CONSUMPTION for 24 months from date of manufacture. Did you know that some years ago a leading consumer magazine looked into the potency of many of our leading probiotic brands with some very concerning results? Nutrigold Pro-otic is one of the first commercially available multi-strain probiotic products on the market which delivers a proven potency at the time of consumption. Furthermore, the Pro-otic formulations are sufficiently stable to allow transport and storage at ambient temperature, avoiding the difficulties and costs of refrigeration. All this and the Pro-otic range is lactose free so suitable for vegetarians and vegans*. FACT: Probiotics have to survive the harsh conditions of the upper digestive tract including stomach acid, bile secretions and pancreatic enzymes before they reach their site of action. It is a vital feature of any probiotic to demonstrate the ability of specific strains to survive and reproduce in conditions similar to those secretions found in the gut. If the probiotic strains cannot survive in sufficient numbers then the supplement is simply a waste of money. The survival in sufficient numbers of the strains of probiotics used in Nutrigold s Pro-otic range have been investigated in a rigorous range of tests simulating the harsh environment of the upper digestive tract. These test areas include: 1. Tolerance of Pro-otic strains to acidic conditions, simulating the acid environment of the stomach, for up to 1.5 hours. 2. Resistance of Pro-otic strains to pepsin, the principal proteolytic enzyme found in gastric acid. 3. Exposure of Pro-otic strains to different concentrations of bile acids and pancreatin, a mixture of several digestive enzymes including amylase, lipase and protease, to mimic conditions in the upper intestinal tract. Bile has bactericidal action so probiotic strains must survive when exposed to bile acids to ensure the probiotic supplement remains viable and not just a waste of money. The in vitro tests show that L. acidophilus CUL-6 strain used in the Nutrigold Pro-otic is highly resistant to acid conditions even after 1.5 hours exposure to acidified (ph2.5). This Lactobacillus strain is also able to survive in the presence of bile acids, pepsin and pancreatin (Figure. 1). The number of viable organisms in each of the four tests that mimic the conditions found in the digestive tract remained the same as the controls. *Our new Mega Strength Pro-otic is as stated, lactose free and stable for 24 months at normal ambient room temperature. However, the current 3.5 billion Pro-otic (NC3, NC9) products may contain traces of lactose so are not considered vegan. However the next batch is estimated to arrive during October/November 211 which will be lactose free and suitable for both vegetarians and vegans. These are estimated dates so for clarification please ask a sales assistant when ordering. A REVOLUTIONARY APPROACH TO PROBIOTIC SUPPLEMENTATION
3 FIGURE 1: In Vitro Tests Demonstrating the Intestinal Survival of L. acidophilus CUL-6 ACID TOLERANCE AT ph2.5 BILE TOLERANCE CUL6 CUL6 in acidified CUL Bile concentration (%) PANCREATIN RESISTANCE PEPSIN RESISTANCE CUL6 CUL6 in intestinal CUL6 CUL6 in gastric Similar results were seen with cultures of L. rhamnosus CUL-63 and B. bifidum CUL-2. A PROVEN POTENCY MULTI STRAIN BACTERIA AT THE TIME OF CONSUMPTION
4 A HIGH POTENCY PROBIOTIC FIGURE 2: In Vitro Tests Demonstrating the Intestinal Survival of B. bifidum CUL-2 ACID TOLERANCE AT ph2.5 BILE TOLERANCE CUL2 CUL2 in acidified CUL Bile concentration (%) PANCREATIN RESISTANCE PEPSIN RESISTANCE CUL2 CUL2 in intestinal CUL2 CUL2 in gastric Pro-otic is one of the fi rst commercially available multi-strain probiotic products on the market which h delivers a proven potency at the time of consumption without the need for inconvenient refrigeration.
5 We feel strongly that with a proven number of viable organisms in each capsule, at the time of consumption, and the survival of the carefully selected probiotic strains in acidic and bile conditions means that our Pro-otic range delivers excellent value for money. FACT: Implantation and adherence to intestinal cells is required for many bowel flora to function. Thorough testing of the Lactobacilli strains found in Pro-otic supplements show that they strongly adhere to human intestinal cells in vitro (Table 1). TABLE 1: Adherence of Lactobacillus Pro-otic Strains to Human Intestinal Cells PRO-OTIC FORMULATIONS The Pro-otic range has been demonstrated to be viable, potent and stable at room temperature making it an excellent probiotic supplement for any naturopath to recommend. The original Pro-otic contains 3.5 billion organisms per capsule guaranteed at time of consumption for up to 24 months. These come in handy pot sizes of 3 or 6 capsules to tailor to your client s requirements. The Mega Strength Pro-otic delivers 25 billion microorganisms at time of consumption proven for 24 months. The capsules are packaged in a handy and easy to use blister pack of 3 capsules. This new packaging offers an excellent solution for storing in cupboards or travelling and also provides a useful reminder for taking your daily dose of Pro-otic. Organism Low Adherence Score Medium Adherence Score High Adherence Score L. acidophilus CUL-6 L. rhamnosus CUL-63 NC195 3 Capsules 1 months supply NC3 6 Capsules 1 2 months supply NC9 3 Capsules 15 3 days supply
6 CHECKLIST FEATURE High potency with proven viability Multi strain of scientifically proven probiotics PRO-OTIC NC9 3.5 billion viable organisms proven from time of consumption L. acidophilus CUL-6 L. rhamnosus CUL-63 L. lactis CUL-72 B. breve CUL-69 B. bifidum CUL-2 B. lactis CUL-72 MEGA STRENGTH PRO-OTIC NC9 25 billion organisms proven from time of consumption L. acidophilus CUL-6 L. rhamnosus CUL-63 L. lactis CUL-72 B. breve CUL-69 B. bifidum CUL-2 B. lactis CUL-72 Hypoallergenic (lactose free) *see note (lactose free) Vegetarian and Vegan *see note Storage Ambient room temperature Ambient room temperature Stability A proven potency 24 months from date of manufacture *see note A proven potency 24 months from date of manufacture Dosage 1-2 capsules daily (as directed) 1 capsule daily Packaging Pot size of 3 or 6 capsules 3 capsules in blister pack *Vegetarian and Vegan: The current Pro-otic (NC9, NC3) products may contain contain traces of lactose so are not considered vegan. However the next batch is estimated to arrive October/November 211 which will be lactose free and suitable for both vegetarians and vegans. These are estimated dates so for clarification please ask a sales assistant when ordering. *Stability: We have recently upgraded our complete Pro-otic range and these products now deliver a proven potency at ambient temperature (no need to refrigerate) for 24 months from the date of manufacture. A real step forward in probiotic supplementation. (The current batch NC9 / NC3 has a proven potency for 15 months). SUGGESTIONS: Use the Pro-otic range as part of a bowel cleansing or detoxification programme alongside other supplements such as Colex or Detox Support formulas as well as in conjunction with Golden Aloe Vera and all the Nutrigold essential fatty acid, vitamin and mineral formulas. NUTRIGOLD Ltd. PO Box No. 217, Exmouth, EX8 9AX Tel: +44() (local rate) International: Fax: +44() talk2us@nutrigold.co.uk Registered Office: Unit 7 Tidwell Court, Liverton Business Park, Exmouth, EX8 2NR Company Reg No VAT Reg No designed by
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