Respiratory Source Collections Source Test Name Additional Names Specimen Requirements Collection Container Processing Information/Transport

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1 Bronchial Wash/Lavage Bronchial Wash/Lavage Bronchial Wash/Lavage Fungal Culture (Dimorphic/ Filamentous) Mycobacterium (TB) Culture Aspergillus Nocardia Streptomyces Blastomyces Coccidioides Cryptococcus Dimorphic fungi Histoplasma Paracoccidoides AFB Culture TB Culture bronchoscopy Collect >1 ml especially if multiple tests are requested If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting as special laboratory safety precautions are required. bronchoscopy Collect >1 ml especially if multiple tests are requested bronchoscopy Collect >1 ml especially if multiple tests are requested container using lid Bronchial Lavage: CUBRONLAVAGE/ Bronchial Lavage C&S Bronchial Wash: CUBRONWASH/ Bronchial Wash C&S including Yeast (Candida) and Aspergillus. Anaerobic cultures are NOT performed on bronchoscopy s. Polymicrobial respiratory infections are best diagnosed by Gram stain. If cell count requested, submit portion of sample to cytology MYCRESP/ Fungus-Respiratory/Bronchial Please indicate if any of the following organisms are specifically requested/suspected: Histoplasma, Blastomyces, Coccidioides or Cryptococcus. Note: For Histoplasma, Blastomyces or Coccidioides requests, serology and/or urine for antigen testing are available. If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting. TBRESP/ TB/Mycobacteria-Respiratory IH West/IH East sites: Mycology Mycobacteria/ TB Page 1 of 17 Respiratory Cultures Mar 2018

2 Bronchial Virus Detection/ Wash/Lavage PCR (NAAT) Bronchial Wash/Lavage Bronchial Wash/Lavage Mycoplasma/ Chlamydophila /Legionella Pneumocystis (PJP/PCP) Adenovirus Bocavirus Coronavirus Enterovirus Human metapneumovirus Influenza MERS-CoV Parainfluenza Rhinovirus RSV (Respiratory syncytial virus) SARS Chlamydophila pneumoniae Legionella species MCL PCR Mycoplasma pneumoniae bronchoscopy Collect >1 ml especially if multiple tests are requested bronchoscopy Collect >1 ml especially if multiple tests are requested bronchoscopy Collect >1 ml especially if multiple tests are requested Check performing site on label: depending on patient location and time of year, s may be tested in an IH site and/or sent to BCCDC VIRRMAG/Virus Flu/Magpix-Bronc/Sputum Magpix includes the following: Influenza/Parainfluenza/RSV/Adenovirus/Co ronavirus/ Bocavirus/ Rhinovirus/Enterovirus/Human metapneumovirus and atypical bacterial pathogens Chlamydophila/ Legionella/ Mycoplasma ** If MERS-CoV or SARS suspected, contact a microbiologist immediately. MCLPCR/ Mycoplasma/Chlamydo/Legionell PCR Order includes testing for Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella species NOTE: In addition the following s/orders are recommended: Urine: Legionella Antigen Submit directly to cytology/histology for fungal stains Virology Mycology Page 2 of 17 Respiratory Cultures Mar 2018

3 Bronchial Pneumocystis Wash/Lavage (PJP/PCP) PCR Bronchoscopy Brush Aspergillus Nocardia Streptomyces bronchoscopy Collect >1 ml especially if multiple tests are requested Requires IH Microbiologist approval. Will only be performed in specific circumstances: Immunocompromised patient with symptoms and radiological findings bronchoscopy using protective sheath brush. Remove from sheath from brush and put into 1mL of sterile saline. St. Paul s Hospital Ship on ice to XSP (St.Paul s Hospital Microbiology) PJPPCR/ Pneumocystis jirovecii PJP PCR **Orderable by Lab only** CUBRONBRUSH/ Bronchial Brush C&S Order includes culture including Yeast (Candida) and Aspergillus. Anaerobic cultures are NOT performed on bronchoscopy s. Bronchoscopy Brush Fungal Culture (Dimorphic/ Filamentous) Blastomyces Coccidioides Cryptococcus Dimorphic fungi Histoplasma Paracoccidoides If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting as special laboratory safety precautions are required.. bronchoscopy using protective sheath brush. Remove from sheath from brush and put into 1mL of sterile saline. MYCRESP/ Fungus-Respiratory/Bronchial Please indicate if any of the following organisms are specifically requested/suspected: Histoplasma, Blastomyces, Coccidioides or Cryptococcus. Note: For Histoplasma, Blastomyces or Coccidioides requests, serology and/or urine for antigen testing are available. If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting. IH West/IH East sites: Mycology Page 3 of 17 Respiratory Cultures Mar 2018

4 Bronchoscopy Mycobacterium AFB Culture Brush (TB) Culture TB Culture Endobronchial biopsy/aspirate bronchoscopy using protective sheath brush. Remove from sheath from brush and put into 1mL of sterile saline. Routine culture is rarely performed. Contact medical microbiologist if required. **Refrigerate **Refrigerate Lab Order Mnemonic/ OM Order Name: TBRESP/ TB/Mycobacteria-Respiratory Lab Order Mnemonic/ OM Order Name: CUENDOBRB/ Bronchial/EBUS Biopsy C&S Mycobacteria/ TB Endobronchial biopsy/aspirate Endobronchial biopsy/aspirate Mycobacterium (TB) Culture Fungal culture (filamentous) AFB Culture TB Culture Mycology Fungus ultrasound guided bronchoscopy. Needle aspiration of tissue/fluid. ultrasound guided bronchoscopy. Needle aspiration of tissue/fluid. **Refrigerate **Refrigerate Lab Order Mnemonic/ OM Order Name: TBRESP/ TB/Mycobacteria-Respiratory MYCRESP/ Fungus-Respiratory/Bronchial Please indicate if any of the following organisms are specifically requested/suspected: Histoplasma, Blastomyces, Coccidioides or Cryptococcus. Note: For Histoplasma, Blastomyces or Coccidioides requests, serology and/or urine for antigen testing are available. If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting Mycobacteria/ TB Mycobacteria/ TB Page 4 of 17 Respiratory Cultures Mar 2018

5 Ear - Canal Drainage Ear - Canal Drainage Esophagus Brush/Biopsy Candida/Yeast Culture Virus Detection/ PCR (NAAT) Aspergillus Swimmer's Ear Otitis Media Otitis Externa Herpes Virus (HSV) Otitis Media: (Middle Ear) Cleanse external ear canal with mild antiseptic. Collect fluid/drainage from middle ear onto swab. Avoid touching contaminating skin with swab. If fluid to be collected by tympanocentis, please see source "Tympanic Fluid" Otitis Externa: (Outer Canal /Swimmer's Ear) Swab of external ear canal/drainage. Otitis Media: (Middle Ear) Cleanse external ear canal with mild antiseptic. Collect fluid/drainage from middle ear onto swab. Avoid touching contaminating skin with swab. If fluid to be collected by tympanocentis, please see source "Tympanic Fluid" Otitis Externa: (Outer Canal /Swimmer's Ear) Swab of external ear canal/drainage. Surgically collected biopsy during endoscopy procedure. Submit tissue/biopsy in small amount of sterile saline or viral transport medium UTM Universal Transport Medium for Virus CUEAR/ Ear C&S -Canal/Drainage Fluid including Yeast (Candida) and Aspergillus. Please note pertinent information such as otitis media, ruptured ear drum, otitis externa, swimmers ear, chronic draining ear or necrotizing malignant otitis externa. If fluid has been collected by tympanocentis, please see source "Tympanic Fluid" If tissue or fluid has been surgically collected, please see source " OR (Intraoperative) s" CUEAR/ Ear C&S -Canal/Drainage Fluid including Yeast (Candida) and Aspergillus. Please note pertinent information such as otitis media, ruptured ear drum, otitis externa, swimmers ear, chronic draining ear or necrotizing malignant otitis externa. If fluid has been collected by tympanocentis, please see source "Tympanic Fluid" If tissue or fluid has been surgically collected, please see source " OR (Intraoperative) s" HERPES PCR/ Herpes PCR- Biopsy/Fld/Bronch Virology Page 5 of 17 Respiratory Cultures Mar 2018

6 Esophagus Candida/Yeast Brush/Biopsy Culture Eye- Conjunctiva Eye- Conjunctiva Eye- Conjunctiva Neisseria gonorrhoeae (GC) Chlamydia trachomatis Biopsy or brushing collected during endoscopy procedure. Remove protective sheath from brush. Submit in small amount of sterile saline. Collect prior to initiating treatment with topical antibiotics or anesthetics. Cleanse skin around eye with mild antiseptic. Use swab pre-moistened with sterile saline and roll over conjunctiva. Collect prior to initiating treatment with topical antibiotics or anesthetics. Cleanse skin around eye with mild antiseptic. Use swab pre-moistened with sterile saline and roll over conjunctiva. If discharge is present, use a sterile swab to clean area. Do not scrape the conjunctiva while cleaning the eye(s). Discard the cleaning swab. Thoroughly swab the inner surface or the lower and upper eyelids. If samples are taken from both eyes, use the swab on the less affected eye first to avoid Obtain special collection kits from laboratory. Dry swab with transport tube CUESOPHA/ Esophagus Brushing C&S Order includes Yeast (Candida) culture CUEYE/ Eye C&S- Conjunctiva/Lacrimal Also includes sources of lacrimal duct If eyelid or cellulitis or, please refer to " Skin - Intact Skin Surface" For surgically collected s, please refer to " Eye- Deep/Surgical" CUESYEGC/ Eye- GC(Gonorrhea) Culture including culture for Neisseria gonorrhoeae (GC) CHLEYE/ Eye- Chlamydia NAAT Testing for Chlamydia trachomatis performed by molecular assay (NAAT/PCR) method. Contact Laboratory for special "dry swab" collection kit Mycology Page 6 of 17 Respiratory Cultures Mar 2018

7 further contamination of that eye. Eye- Conjunctiva Virus Detection/ PCR (NAAT) Adenovirus Herpes Virus (HSV) Varicella Virus (VSV) Pink Eye (Adenovirus) Using swab from viral transport kit, gently sweep/ roll over conjunctiva. Place swab directly into viral transport medium. UTM Universal Transport Medium for Virus VIRSKIN/ Virus- Skin/Lesion/Wound Virology Mouth Vincent's Organisms Also includes sources of tongue or gums. Have patient rinse mouth with water prior to collection. Swab mucosal surface of gums. CUMOUTH/ Mouth/Tongue C&S - Candida/Yeast If Vincent's angina requested: CUMOUTHVO/ Mouth/Tongue C&S- Vincent's Order includes culture for Yeast (Candida) (Thrush) Gram stain for Vincent's organisms will be performed if specifically requested. Please indicate any pertinent clinical information such as failed therapy, immunocompromised, oncology or cancer patient. If dental or tooth abscess swab, please refer to "Wound - Abscess (Pus) Swab" Mouth Candida/ Yeast Culture Thrush Also includes sources of tongue or gums. Have patient rinse mouth with water prior to collection. Swab mucosal surface of gums. CUMOUTH/ Mouth C&S - Candida/Yeast Order includes culture for Yeast (Candida) (Thrush) Please indicate any pertinent clinical information such as failed therapy, immunocompromised, oncology or cancer patient. If dental or tooth abscess swab, please refer to "Wound - Abscess (Pus) Swab" Page 7 of 17 Respiratory Cultures Mar 2018

8 Mouth Virus Detection/ UTM Universal Transport PCR (NAAT) Medium for Virus Nasal Sinus- Aspirate/Lavage Nasal Sinus- Aspirate/Lavage Fungal Culture (Dimorphic/ Filamentous) Herpes Virus (HSV) Varicella Virus (VSV) Aspergillus Includes vesicular lesions on lips (cold sore) lesion, mouth or skin around mouth or nose. Unroof the lesion with a tuberculin syringe or broken edge of a sterile swab shaft. Swab the broken blister and place swab into transport media. Alternately, the contents of the lesion may be aspirated with the syringe and transferred directly to the vile of transport medium. Antral aspirate or lavage. If surgically collected tissue or fluid, please refer to source "OR (Intraoperative) s" Antral aspirate or lavage. If surgically collected tissue or fluid, please refer to source "OR (Intraoperative) s" VIRSKIN/ Virus- Skin/Lesion/Wound CUNASALSIN/ Nasal Sinus C&S- Swab/Lavage including Yeast (Candida) and Aspergillus. Anaerobic culture only performed on fluid/aspirated s. If surgically collected tissue or fluid, please refer to source "OR (Intraoperative) s" MYCRESP/ Fungus-Respiratory/Bronchial Virology IH West/IH East sites: Mycology Nasal Sinus - Swab Candida/Yeast Culture Swab of maxillary nasal sinus. Nasal sinus swabs are suboptimal s. Recommend that tissue, fluid or lavage be submitted CUNASALSIN/ Nasal Sinus C&S- Swab/Lavage including Yeast (Candida) and Aspergillus. Page 8 of 17 Respiratory Cultures Mar 2018

9 Nasal Sinus - Aspergillus Swab Nasopharynx Nasopharynx Nasopharynx Bordetella pertussis Virus Detection/ PCR (NAAT) Pertussis Whooping Cough Adenovirus Bocavirus Coronavirus Enterovirus Human metapneumovirus Influenza Parainfluenza Rhinovirus RSV (Respiratory syncytial virus) Swab of maxillary nasal sinus. Nasal sinus swabs are suboptimal s. Recommend that tissue, fluid or lavage be submitted Specialized collection kit. Collect pernasal/ nasopharyngeal sample as per the collection kit instructions. Place wire shaft into transport media. Nasopharyngeal swabs/washes are not processed for routine culture as there is poor correlation between organisms in the nasopharynx and the etiologic agents of bacterial sinusitis. Nasopharyngeal flocked swab or Nasopharyngeal wash Copan 125C - Amies Charcoal Transport Medium with wire shaft swab. Contact Laboratory for specialized collection kits UTM Universal Transport Medium for Virus ** Refrigerate Check performing site on label: depending on patient location and time of year, s may be tested in an IH site and/or sent to BCCDC CUNASALSIN/ Nasal Sinus C&S- Swab/Lavage including Yeast (Candida) and Aspergillus. Anaerobic culture only performed on fluid/aspirated s. PERTUSSIS/ Pertussis (Whooping Cough) Lab Order Mnemonic/ OM Order Name: If patient 2 years: VIRRSV/ Virus RSV Nasopharynx ( 2 years) Negative and Inconclusive s are sent to BCCDC for Magpix Testing If patient 3-4 years: VIRMAGN/Virus Flu/RSV/Magpix- Nasopharynx If patient 5 years and an outpatient: VIRRESP/Virus Flu A/B - Outpatient If patient 5 years and an inpatient: VIRRESPN/ Virus Flu A/B -Nasopharynx If physician specifically orders Magpix: VIRRMAGN/Virus Flu/RSV/Magpix - Nasopharynx "Magpix testing includes: Influenza/ Parainfluenza/ RSV/ Adenovirus/ Coronavirus/ Bocavirus/ Rhinovirus/ Enterovirus/ Human metapneumovirus and Mycology Virology Page 9 of 17 Respiratory Cultures Mar 2018

10 Nasopharynx Mycoplasma/ UTM Universal Transport Ureaplasma Medium for Virus Nose/Nares Nose/Nares Nose/Nares Cardiac/Surgical Nasal Screen MRSA Screen (ARO) Staph carrier Staph carrier Pre-surgical screen Methicillin Resistant Staph aureus The investigation of "genital" mycoplasmas (Mycoplasma hominis/ Ureaplasma urealyticum) will only be performed on neonates Collect nasopharyngeal flocked swab or at least 1mL of nasopharyngeal aspirate. Transfer into viral transport medium. Also includes sources of nares, nostril. Insert swab into the nares and rotate against the nasal mucosa. Nasal secretions are not suitable s and will not be processed. If swab of rash/lesion/sore, please refer to " Skin - Intact Skin Surface" Pre-op cardiac OR orthopedic surgery screening for Staphylococcus aureus and MRSA. Insert swab into the nares and rotate against the nasal mucosa. Use one swab to swab both nares. Insert swab into the nares and rotate against the nasal mucosa. Repeat in other nostril. ** Refrigerate atypical bacterial pathogens Chlamydophila/ Legionella/ Mycoplasma GENMYCO PCR/ Genital Mycoplasma/ Ureaplasma CUNOSE / Nose/Nasal C&S Routine nose cultures only processed for presence of Staphylococcus aureus. If swab of rash/lesion/sore, please refer to " Skin - Intact Skin Surface" CUCARDSCRN/ Nose/Nasal C&S- Cardiac Screen CUMRSANOSE/ ARO MRSA Nose/Nares Order includes screening culture for Methicillin Resistant strains of Staphylococcus aureus (MRSA) Mycology Page 10 of 17 Respiratory Cultures Mar 2018

11 Nose/Nares Corynebacterium Diphtheria diphtheriae Sputum Tracheal Aspirate ETT Nocardia Aspergillus Burkholderia pseudomallei (Meloidosis) Specialized collection kit. Insert swab into the nares and rotate against the nasal mucosa. Recovery of C.diphtheriae is enhanced by culturing both a nose and a throat sample. Please submit two s. **IH Patient Collection Instructions available for sputum collections** Have patient rinse mouth with water or saline (not mouth wash) prior to collection. Instruct patient to cough deeply and produce a lower respiratory, NOT saliva. Best quality sample is first morning. Also includes sputum collected by auger/endotracheal suction/tracheal aspirate. If Burkholderia pseudomallei is suspected, please contact microbiologist before collecting, as special laboratory precautions are required. Copan 125C - Amies Charcoal Transport media Contact Laboratory for specialized collection kits delivered directly to microbiology lab for sterile splitting of ** Refrigerate DIPHTHC/ Diphtheria Culture CUSPUTUM/ Sputum C&S including Yeast/Candida and Aspergillus Please note any pertinent clinical information on order such as COPD, bronchiectasis, hemoptysis immunosuppression or oncology/ cancer patient. If tracheostomy site swab, please order as "Wound - Broken Skin" If Burkholderia pseudomallei is suspected, please contact microbiologist. Mycology Page 11 of 17 Respiratory Cultures Mar 2018

12 Sputum Candida/Yeast Culture Sputum Cystic Fibrosis (CF) Culture Burkholderia cepacia **IH Patient Collection Instructions available for sputum collections** Have patient rinse mouth with water or saline (not mouth wash) prior to collection. Instruct patient to cough deeply and produce a lower respiratory, NOT saliva. Best quality sample is first morning. Also includes sputum collected by auger/endotracheal suction/tracheal aspirate **IH Patient Collection Instructions available for sputum collections** Have patient rinse mouth with water or saline (not mouth wash) prior to collection. Instruct patient to cough deeply and produce a lower respiratory, NOT saliva. Best quality sample is first morning. Also includes sputum collected by auger/endotracheal suction/tracheal aspirate supplied ** Refrigerate ** Refrigerate CUSPUTUM/ Sputum C&S including Yeast/Candida and Aspergillus Please note any pertinent clinical information on order such as COPD, bronchiectasis, hemoptysis immunosuppression or oncology/ cancer patient. If tracheostomy site swab, please order as "Wound - Broken Skin" CUSPUTUMCF/ Sputum C&S - Cystic Fibrosis Only one per week should be processed due to complexity and extended incubation of CF cultures. Page 12 of 17 Respiratory Cultures Mar 2018

13 Sputum Fungal Culture Sputum s are (Dimorphic/Filam not processed for fungal entous) culture. If Yeast/Candida or Aspergillus culture requested, order C&S routine culture. If dimorphic fungi suspected, submit bronchoscopy or tissue, or consult microbiologist Sputum Sputum Mycoplasma/ Chlamydophila /Legionella CPO Screen (ARO) (outbreak/ exposure) Chlamydophila pneumoniae Legionella species MCL PCR Mycoplasma pneumoniae Carbapenemase producing organism **IH Patient Collection Instructions available for sputum collections** Have patient rinse mouth with water or saline (not mouth wash) prior to collection. Instruct patient to cough deeply and produce a lower respiratory, NOT saliva. Best quality sample is first morning. Also includes sputum collected by auger/endotracheal suction/tracheal aspirate **IH Patient Collection Instructions available for sputum collections** Also includes sputum collected by auger/endotracheal suction/tracheal aspirate ** Refrigerate ** Refrigerate 12 hours MCLPCR/ Mycoplasma/Chlamydo/Legionell PCR Order includes testing for Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella species NOTE: In addition the following s/orders are recommended: Serum: Mycoplasma IGM Urine: Legionella Antigen CUCPOOUTBREAK/ARO CPO Xposure Order includes screening culture for Carbapenemase producing organisms Mycology Page 13 of 17 Respiratory Cultures Mar 2018

14 Sputum Mycobacterium AFB Culture (TB) Culture TB Culture Sputum Throat/Tonsil Parasite Exam/ Identification Paragonimus Strongyloides Toxoplasma Echinococcus Arcanobacterium Group A Streptococcus Strep Throat **IH Patient Collection Instructions available for sputum collections** Have patient rinse mouth with water or saline (not mouth wash) prior to collection. Instruct patient to cough deeply and produce a lower respiratory, NOT saliva. Best quality sample is first morning. Typically 3 separate collections on consecutive days are requested. Also includes sputum collected by auger/ endotracheal suction/ tracheal aspirate Collect sputum into sterile screw cap container Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa (s). Typically 3 s are collected on consecutive mornings. container using supplied lid. container using supplied lid. ** Refrigerate ** Refrigerate TBRESP/ TB/Mycobacteria-Respiratory PARAW/ Parasite-Worm/Fecal Object ID Specify sample type and suspected parasite name CUTHROAT / Throat C&S (Group A Strep) Cultured routinely for Group A Streptococcus. Please indicate if Yeast (Candida) specifically requested. Note any pertinent clinical information such as rash, epiglottis, treatment failure, penicillin allergy or immunosuppression. Throat cultures are not processed for Neisseria meningitidis - submit blood if patient is systemically unwell. If tonsillar abscess swab, please refer to "Wound- Abscess (Pus) Swab" Mycobacteria/TB Parasitology Page 14 of 17 Respiratory Cultures Mar 2018

15 Throat/Tonsil Candida/Yeast Culture Throat/Tonsil Throat/Tonsil Throat/Tonsil Cystic Fibrosis (CF) Culture Neisseria gonorrhoeae (GC) Chlamydia trachomatis Burkholderia cepacia STI gonorrhea Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa Note: In high risk populations such as MSM (men who have sex with men) a pharyngeal NAAT test may be collected in addition to culture. Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa High risk populations: Aptima Unisex swab kit (CT/GC NAAT) Aptima Unisex swab kit Store at room temperature CUTHROAT / Throat C&S (Group A Strep) Please indicate if Yeast (Candida) specifically requested. Note any pertinent clinical information such treatment failure or immunosuppression. CUTHROATCF/ Throat C&S-Cystic Fibrosis Throat swabs for CF work-up are acceptable in patient who are less than 10 years old. For patients over 10 years old, please submit a sputum sample. Only one per week should be processed due to complexity and extended incubation of CF cultures. CUTHROATGC/ Throat- Gonorrhea (GC) Culture Routine throat culture including Neisseria gonorrhoeae (GC) If Aptima (NAAT) test collected CHLGCTHROAT / Throat-Chlamydia/GC NAAT CHLTHROAT/ Throat- Chlamydia NAAT Testing for Chlamydia trachomatis performed by molecular assay (NAAT/PCR) method If Aptima swab: Mycology Mycology Page 15 of 17 Respiratory Cultures Mar 2018

16 Throat/Tonsil Virus Detection/ Enterovirus UTM Universal Transport PCR (NAAT) Medium for Virus Throat/Tonsil Corynebacterium diphtheriae Diphtheria Depress the tongue with a tongue depressor. Using conventional swab, sample the posterior pharynx, tonsils, and inflamed areas. Specialized collection kit. Swab the posterior pharynx, tonsils. Recovery of C.diphtheriae is enhanced by culturing both a nose and a throat sample. Please submit two s. Copan 125C - Amies Charcoal Transport media Contact Laboratory for specialized collection kits ENTEROVPCR/ Enterovirus PCR In suspected meningitis/encephalitis cases when a CSF cannot be obtained, a throat and a stool should be submitted. DIPHTHC/ Diphtheria Culture Virology Mycology Throat/Tonsil Transbronchial tissue biopsy Transbronchial tissue biopsy Rapid Strep Antigen Test Mycobacterium (TB) Culture Strep A antigen Rapid Strep AFB Culture TB Culture Depress the tongue with a tongue depressor. Sample the posterior pharynx, tonsils, and inflamed areas. Avoid swabbing tongue or oral mucosa. Bloody s can lead to an invalid result. Note: In children (<18 years) a negative result should be confirmed with a throat culture. Routine culture is rarely performed. Contact medical microbiologist if required. bronchoscopy. Collection of tissue using forceps. Rayon-tipped swabs with plastic shafts (provided in the test kit). Obtain from the lab. Transport in a clean dry plastic tube. ASAP CUTHROATDST/ Throat- Rapid Grp A Antigen Lab Order Mnemonic/ OM Order Name: CUENDOBRB/ Bronchial/EBUS Biopsy C&S Lab Order Mnemonic/ OM Order Name: TBRESP/ TB/Mycobacteria-Respiratory Mycobacteria/ TB Page 16 of 17 Respiratory Cultures Mar 2018

17 Transbronchial Fungal culture Mycology tissue biopsy (filamentous) Fungus Transbronchial needle aspiration Transbronchial needle aspiration Transbronchial needle aspiration Mycobacterium (TB) Culture Fungal culture (filamentous) AFB Culture TB Culture Mycology Fungus ultrasound guided bronchoscopy. Needle aspiration of tissue/fluid. Routine culture is rarely performed. Contact medical microbiologist if required. bronchoscopy. Fluid collection using needle aspiration. bronchoscopy. Fluid collection using needle aspiration. MYCRESP/ Fungus-Respiratory/Bronchial Please indicate if any of the following organisms are specifically requested/suspected: Histoplasma, Blastomyces, Coccidioides or Cryptococcus. Note: For Histoplasma, Blastomyces or Coccidioides requests, serology and/or urine for antigen testing are available. If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting Lab Order Mnemonic/ OM Order Name: CUENDOBRB/ Bronchial/EBUS Biopsy C&S Lab Order Mnemonic/ OM Order Name: TBRESP/ TB/Mycobacteria-Respiratory MYCRESP/ Fungus-Respiratory/Bronchial Please indicate if any of the following organisms are specifically requested/suspected: Histoplasma, Blastomyces, Coccidioides or Cryptococcus. Note: For Histoplasma, Blastomyces or Coccidioides requests, serology and/or urine for antigen testing are available. If Blastomyces, Coccidioides, Histoplasma or Paracoccidoides suspected, consult microbiologist before collecting Mycobacteria/ TB Mycobacteria/ TB Mycobacteria/ TB Page 17 of 17 Respiratory Cultures Mar 2018