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1 DEPARTMENT OF SCIENCE COURSE OUTLINE Fall 2015 BC 2000 INTRODUCTORY BIOCHEMISTRY INSTRUCTOR: Philip Johnson PHONE: OFFICE: J224 OFFICE HOURS: Tuesdays hrs & hrs Thursdays hrs PREREQUISITE(S)/COREQUISITE: CH 1010 and CH 1610 or CH 2610 REQUIRED TEXT/RESOURCE MATERIALS: Essential Biochemistry (3 rd Edition, 2014) Charlotte W. Pratt and Kathleen Cornely John Wiley & Sons Inc. Publishers SUPPLEMENTS: Practice quizzes will be made available on Moodle to aid preparation for exams. These can be completed at any time by students. A link to the textbook website is also available on Moodle. This site provides access to a number of useful resources. CALENDAR DESCRIPTION: An introduction to the fundamental principles of biochemistry. Protein structure and function; lipids and the structure of biological membranes; nucleotides and the structure of nucleic acids; bioenergetics and the metabolism of carbohydrates, lipids and nitrogen; the integration and regulation of cellular metabolism. This course is designed both for students who require a single term course in the fundamental principles of biochemistry, AND for students who intend to take further courses in biochemistry. LEARNING OUTCOMES: 1. Students should know the reactions of common metabolic pathways. 2. Students should understand how metabolic pathways are integrated. 3. Students should understand the relationship between metabolism and disease. COURSE OBJECTIVES: 1. To gain an understanding of biochemical reactions and pathways. 2. To develop critical thinking skills with respect to biochemistry. Copyright 2009, Grande Prairie Regional College and its licensors. 1
2 CREDIT/CONTACT HOURS: 3 credits (3-0-0) DELIVERY MODE(S): Classes Mondays (J226) Fridays (J226) TRANSFERABILITY: University of Alberta (Biochemistry 200) University of Calgary (Biochemistry 341) EVALUATIONS: Mid-term Exam I 30% Mid-term Exam II 30% Final Exam 40% GRADING CRITERIA: GRANDE PRAIRIE REGIONAL COLLEGE GRADING CONVERSION CHART Alpha Grade 4-point Percentage Designation A + Equivalent 4.0 Guidelines A EXCELLENT A B FIRST CLASS STANDING B B GOOD C C SATISFACTORY C D D MINIMAL PASS F FAIL WF FAIL, withdrawal after the deadline ** Grade of D or D+ may not be acceptable for transfer to other post-secondary institutions. Students are cautioned that it is their responsibility to contact the receiving institutions to ensure transferability Copyright 2009, Grande Prairie Regional College and its licensors. 2
3 STUDENT RESPONSIBILITIES: Refer to the College Policy on Student Rights and Responsibilities at STATEMENT ON PLAGIARISM AND CHEATING: Refer to the College Student Misconduct: Academic and Non-Academic Policy at **Note: all Academic and Administrative policies are available at All cell phones should be switched off while students are in class. Should a cell phone ring during class, the first instance will result in a warning to all students; further instances will results in the owner of the cell phone being asked to leave that day s class. Students will be allowed to use standard non-programmable calculators in exams. All other electronic devices are prohibited and should not be brought into exams. Students found to be using a prohibited electronic device during an exam will be required to leave immediately and will receive a mark of zero for that exam. Copyright 2009, Grande Prairie Regional College and its licensors. 3
4 BC 2000 Fall Topic Outline Biological Molecules Topic Textbook Readings (pages) 1 st Ed. 2 nd Ed. 3 rd Ed. Types of biomolecules Biopolymers Nucleosides and nucleotides Basic structure of DNA and RNA Functions of Nucleic acids (Central Dogma) Protein Structure and Function Overview Amino acids Peptide bonds and primary structure Secondary structures Tertiary structure and stabilization Protein folding & Quarternary structure Oxygen binding to myoglobin and haemoglobin Lipids and Biological Membranes Fatty acids, triacylglycerols and membrane lipids Lipid bilayers and membrane fluidity Membrane proteins Fluid Mosaic Model Passive and active membrane transport Porins, ion channels and gated channels The Na + -K + ATPase and Na + -glucose transporters Introduction to hormones and receptors Enzymes What is an enzyme? Classifying enzymes How do enzymes work? Catalytic mechanisms Substrate binding Copyright 2009, Grande Prairie Regional College and its licensors. 4
5 54-56, Co-enzymes and dietary vitamins Enzyme kinetics Enzyme inhibition Allosteric enzymes 219, Other in vivo regulatory mechanisms Co-enzymes and roles as electron carriers Introduction to Metabolism Energy and metabolism Food and Fuel Storage and use of fuels Organ specialization Class notes Class notes Class notes Metabolic pathway and common intermediates Oxidation and reduction Overview of metabolism Free energy changes in metabolic reactions , Energy currency, ATP, coupled reactions Fig 3-4a Fig 3-3a Glucose Metabolism Introduction , Glycolysis , Fates of Pyruvate 320, Anaerobic exercise and the Cori Cycle Gluconeogenesis and Glycogen synthesis Pentose phosphate pathway Summary of glucose metabolism Hormonal regulation Citric Acid Cycle and Oxidative Phosphorylation Introduction Conversion of pyruvate to acetyl-coa Reactions of the Citric Acid Cycle Copyright 2009, Grande Prairie Regional College and its licensors. 5
6 Regulation of the Citric Acid Cycle Catabolism, anabolism and anapleurotic reactions Overview of oxidative phosphorylation Mitochondria and Electron transport chain Chemiosmosis ATP synthase ATP yield from aerobic catabolism of glucose Metabolism of Fats, Fatty Acids and Cholesterol Overview of fat metabolism Fig Fig Fig Transport of lipids TAG synthesis Lipases and TAG breakdown Degradation of fatty acids (activation and transport Degradation of fatty acids (β-oxidation) Glyoxylate cycle Fatty acid synthesis Regulation of fatty acid metabolism Fat metabolism and diabetes Ketone bodies and ketogenesis Cholesterol synthesis and regulation Fates of cholesterol , Good and Bad cholesterol Box 14A Box 17A Box 17A Nitrogen Metabolism Nitrogen fixation and assimilation Transamination and amino acid synthesis , Catabolism of amino acids The Urea Cycle and nitrogen disposal Copyright 2009, Grande Prairie Regional College and its licensors. 6
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