Section Lectures: Immunology/Virology Time: 9:00 am 10:00 am LRC 105 A & B

Transcription

1 Section Director: Cliff Bellone, Ph.D. Office: Doisy Hall - R 405 Phone: bellonec@slu.edu Lecturers: James Swierkosz, Ph.D. Office: Medical School Rm. 412 Phone: swierkoszje@slu.edu Jacki Kornbluth, Ph.D. Office: Desloge Towers 11 th Floor Phone: kornblut@slu.edu Lynda Morrison, Ph.D. Office: Medical School Rm. 431 Phone: morrisla@slu.edu Dan Hoft, M.D., Ph.D. Office: Desloge Towers 8 th Floor Phone: hoftdf@slu.edu John Tavis, Ph.D. Office: Doisy Research R421 Phone: tavisje@slu.edu R. Mark L. Buller, Ph.D. Office: Medical School Rm. 432 Phone: bullerrm@slu.edu Section Lectures: Immunology/Virology Time: 9:00 am 10:00 am LRC 105 A & B

2 Lecture 1: March 17, 2003 Lecture 2: March 18, 2003 Lecture 3: March 19, 2003 Lecture 4 March 20, 2003 Lecture 5 March 21, 2003 Dr. Swierkosz Elements of the Immune System Components of innate and adaptive immunity Hematopoiesis Development of lymphoid cells in primary and secondary lymphoid organs Adaptive Immunity: Receptors, Recognition, Clonal Selection, and Cell Activation Effector functions of B and T lymphocytes Effective versus Comprised Immune Responses Required Reading: Janeway, Ch th ed. Dr. Kornbluth Innate Immunity/Inflammation I, II Early pathogen-host interactions, host response to injury/infection (inflammation), phagocytosis, inflammatory mediators, characteristics and functions of natural killer (NK) cells, mechanisms of NK killing, NK receptors involved in recognition. Recommended Reading: Janeway 5 th ed: chapter 1, pgs , chapter 2, pgs 35-44, 64-69, Dr. Kornbluth Innate Immunity/Inflammation I, II Early pathogen-host interactions, host response to injury/infection (inflammation), phagocytosis, inflammatory mediators, characteristics and functions of natural killer (NK) cells, mechanisms of NK killing, NK receptors involved in recognition. Recommended Reading: Janeway 5 th ed: chapter 1, pgs , chapter 2, pgs 35-44, 64-69, Immunoglobulins (Ig), Ig Genes, and Ig Diversity Definition and historical perspective, Immunoglobulin structure and function, myelomas, antigen binding structure, antigenantibody interactions, Ig classes (isotypes) and function, Ig genes genomic organization and mechanisms to generate antibody diversity. Required Reading: Janeway, 5 th edition, chapter 3,4, and 7, pp T cell receptor (TCR), TCR Genes, and TCR Diversity TCR structure and function, historical perspective, TCR ligand, TCR co-receptors, TCR genes genomic organization and mechanisms to generate TCR diversity.

3 Required Reading: Janeway, 5th edition, chapters 3,4, and 7, pp Lecture 6 March 24, 2003 Lecture 7 March 25, 2003 Lecture 8 March 26, 2003 Lecture 9 March 27, 2003 Dr. Tavis Virology: Why should you care & The early events What s a virus? Overview of viral structures, rationale of viral taxonomy, general concepts of viral replication strategies, entry and tropism, and virus/host population dynamics. Required Reading: Chapters 1 and 2 (pp. 1-56) in Principles of Virology, Flint et al, editors (copies on reserve in library). NOTE: be sure to finish Chapter 2 before the Special Topics session on March 19. Dr. Tavis Viral gene expression strategies or Weird stuff in the cell! Viral coding capacity limitations, dependence of expression strategy on viral replication scheme, transcription from nuclear and cytoplasmic DNA viruses, transcription from RNA viruses, viral RNA splicing, viral RNA transport, cap-free translation, and unusual translation strategies. Required Reading: Chapters 1 and 2 (pp. 1-56) in Principles of Virology, Flint et al, editors (copies on reserve in library). NOTE: be sure to finish Chapter 2 before the Special Topics session on March 19. Dr. Tavis Viral genomic replication strategies or Even weirder stuff in the Cell! Double-stranded DNA viruses, single-stranded DNA viruses, positive polarity RNA viruses, minus-polarity RNA viruses, double-stranded RNA viruses, reverse transcribing viruses, viroids, multi-segmented viruses, quasi-species, recombination, and evolution. Required Reading: Chapters 1 and 2 (pp. 1-56) in Principles of Virology, Flint et al, editors (copies on reserve in library). NOTE: be sure to finish Chapter 2 before the Special Topics session on March 19. Dr. Lynda Morrison Viral Exit and Persistence Strategies Viron assembly, envelopment; cytolytic exit, budding, or cell-cell spread; polarized transport; acute, chronic or latent infections and real-life examples of each. Required Reading: Roizman, B Redefining Virology. Science 288: Bresnahan, W.A, and T. Shenk

4 Science 288: Blau, DM, and RW Compans Polarization of viral entry and release in epithelial cells. Sem. Virol. 7: Lecture 10 March 28, 2003 Lecture 11 March 31, 2003 Lecture 12 April 1, 2003 Dr. Morrison HIV Pathogenesis and immune response acute, asymptomatic and symptomatic phases; HIV strategies of immune evasion. Required Reading: Soudeyns, H, and G. Pantaleo The moving target: mechanisms of HIV persistence during primary infection. Imm. Today 20: TCR and T Cell Development Historical perspective, programmed development in thymus, developmental markers, positive and negative selection (development of T cell repertoire), tools to study T cell development florescent activated cell sorter (FACS) and TCR transgenic mice. Required Reading: Janeway, 5 th edition, chapter 7. The MHC and Antigen Presentation Definition of the MHC, historically and otherwise; genetics and protein structure; MHC class I pathway of antigen processing and presentation; MHC class II pathway of antigen processing and presentation. Required Reading: Janeway, 5 th edition, chapter 5 Lecture 13 April 2, 2003 Lecture 14 April 3, 2003 T Cell Effector Functions T cell activation, proliferation, and differentiation. T cell subpopulations and effector functions. Required Reading: Janeway, 5 th edition, chapter 8, 9, pp Dr. Dan Hoft Immunity to bacterial and other pathogens. Host adaptive immune response to different pathogens biology of the pathogen determines mechanisms of protective immunity. Required reading: Janeway, 5 th edition, chapter 10

5 Lecture 15 April 4, 2003 Lecture 16 April 7, 2003 Lecture 17 April 8, 2003 Dr. Mark Buller Taming the Virus The use of viruses as vectors for vaccine, gene therapy and cancer treatment. Each vector system will be discussed from a biological perspective eg what properties of the virus replication cycle facilitated its development as a vector. Required Reading: Principles of Virology, Flint et al., pages 51 to 55; 678. Dr. Mark Buller New, Emerging and Reemerging Microbial Pathogens Overview of the historical importance of infectious diseases in medicine. Properties of microbes which enhance their ability to colonize human populations will be discussed. Also we will describe the role of human activity on planet earth in this process. Required reading: Principles of Virology, Flint et al., pages 716 to 746. Dr. Lynda Morrison Virus v. Host: cellular pathology and defense Virus-induced pathology; apoptosis; interferons; nitric oxide; innate immune defenses. Required Reading; Kalvakolanu, DV Virus interception of cytokine-regulated pathways. Trends in Micro. 7: Miller, LK, and E. White Apoptosis in virus infection. Sem. Virol. 8: Lecture 18 April 9, 2003 Dr. Lynda Morrison Host v. Virus: defense and disease Time course of immune response; induction, effector and memory phases and participants; tailoring of immune responses to infectious agents. Required Reading: Cerny, A, and FV Chisari Pathogenesis of chronic Hepatitis C: Immunological features of hepatic injury and viral persistence. Hepatology 30: Nash, AA, EJ Usherwood, and JP Stewart Immunological features of murine gammaherpesvirus infection. Sem. Virol April 10, 2003 April 11, 2003 Open Day Exam 9:00 am - Noon

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